Enzymatic degradation of polyethylene terephthalate

Abstract

The present disclosure provides variant enzymes for use in the enzymatic degradation of polyethylene terephthalate (PET).

Description

[0001]AƩorney Docket No.129436-5004-WO ENZYMATIC DEGRADATION OF POLYETHYLENE TEREPHTHALATE BACKGROUND OF THE INVENTION The use of polyethylene terephthalate (PET) is widespread and ubiquitous. As such, PET is a major source of environmental polluƟon globally. Physical recycling is possible but underused in many seƫngs. EnzymaƟc degradaƟon has been explored in the last decades but has proven challenging. Provided herein are variant enzymes for use in the enzymaƟc degradaƟon of PET. BRIEF SUMMARY OF THE INVENTION In one aspect, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 8, 12, 16, 20, 23, 24, 69, 89, 92, 109, 110, 112, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 212, 213, 215, 216, 218, 222, 223, 229, 246, 248 and 249, wherein said variant Bhr- PETase has at least 85% idenƟty to SEQ ID NO:1 and has PETase acƟvity. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 12, 20, 23, 24, 69, 89, 92, 109, 110, 157, 170, 174, 176, 177, 181, 182, 183, 187, 203, 206, 208, 212, 215, 216, 222, 229, 246 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 8, 16, 112, 173, 204, 213, 218, 223 and 248. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 20, 23, 24, 69, 89, 92, 109, 110, 157, 170, 174, 176, 177, 181, 182, 183, 187, 203, 206, 208, 212, 215, 216, 222, 229, 246 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 8, 16, 112, 173, 204, 213, 218, 223 and 248. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 8, 12, 16, 20, 23, 24, 69, 89, 92, 109, 110, 112, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 212, 213, 215, 216, 218, 222, 223, 229, 246, 248 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, S27L, R12E, P20T, V23T, A24V, W69H, W69I, W69L, W69S, R89K, R89M, L90A, F92G, T109L, S110R, T157A, T157G, T157L, T157Q, V170I, V170L, A174G, A174R, T176S, V177I, V177T, S181T, Q182E, H183S, H183T, F187I, F187L, D203A, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, T206D, F208D, F208G, F208S, F208T, S212Q, A215D, A216D, A216E, A216T, I222L, V229I, A246Q and D249L. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of R12T, W69C, W69F, W69G, T157D, A174K, S181D, H183A, H183D, H183Y, F187V, F208I, F208K, F208Q, F208V and S212N. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of P8L, T16A, P112Q, E173N, E173P, E173T, E173V, E173W, N204G, P213D, S218G, S218R, S223N and S248R. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of said amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, S27L, P8L, R12E, R12T, T16A, P20T, V23T, A24V, W69C, W69F, W69G, W69H, W69I, W69L, W69S, R89K, R89M, L90A, F92G, T109L, S110R, P112Q, T157A, T157D, T157G, T157L, T157Q, V170I, V170L, E173N, E173P, E173T, E173V, E173W, A174G, A174K, A174R, T176S, V177I, V177T, S181D, S181T, Q182E, H183A, H183D, H183S, H183T, H183Y, F187I, F187L, F187V, D203A, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, T206D, F208D, F208G, F208I, F208K, F208Q, F208S, F208T, F208V, S212N, S212Q, P213D, A215D, A216D, A216E, A216T, S218G, S218R, I222L, S223N, V229I, A246Q, S248R and D249L. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, P20T, V23T, A24V, W69H, A174R, T176S, V177T, H183T, D203A, D203G, D203S, F208D, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, W69F and S181D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203G, D203S, F208D, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, seventeen of said posiƟons, eighteen of said posiƟons, nineteen of said posiƟons or twenty of said posiƟons. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of S27L / L90Y / A174G / D203V / D249L, S27L / A174G / D203L / D249L, S27L / L90A / A174G / D203R / D249L, S27L / L90Y / A174G / D203N, S27L / L90Y / A174G / D249L, S27L / L90Y / A174G / D203G / D249L, S27L / L90Y / D203L / D249L, S27L / D203L / D249L, S27L / L90A / A174G / D203A / D249L, S27L / L90A / A174G / D203Y, S27L / L90Y / A174G / D203A / D249L, S27L / L90Y / A174G / D203L / D249L, S27L / L90Y / D203A / D249L, S27L / L90Y / D203Y / D249L, S27L / L90Y / D203K / D249L, S27L / L90Y / D249L, S27L / A174G / D249L, S27L / L90Y / A174G / D203K / D249L, S27L / L90Y / A174G / D203Y / D249L, S27L / L90Y, S27L / I222L, S27L / I222L / V229I, P20T / V23T / A24V / S27L / T109L / I222L / V229I, P20T / V23T / S27L / I222L / V229I, P20T / S27L / T109L / I222L / V229I, P20T / A24V / S27L / I222L / V229I, P20T / V23T / S27L / T109L / S110R / I222L / V229I, A24V / S27L / I222L / V229I, P20T / V23T / A24V / S27L / S110R / I222L / V229I, S27L / T109L / I222L, P20T / S27L / I222L / V229I, S27L / T109L / I222L / V229I, P20T / V23T / A24V / S27L / T109L / I222L, A24V / S27L / T109L / I222L / V229I, P20T / S27L, P20T / V23T / S27L / S110R / I222L / V229I, R12E / S27L / D203R, S27L / F92G / T157Q / V170I, S27L / R89M / T157G / S181T / Q182E, S27L / T157Q / V170L / T176S / V177T / A215D / A216T, V23T / A24V / S27L / T109L / S110R / I222L / V229I, P20T / A24V / S27L / T109L / S110R / I222L / V229I, P20T / V23T / A24V / S27L / T109L / S110R / V229I, P20T / V23T / A24V / S27L / T109L / S110R / I222L, S27L / T206D, S27L / V177I / A215D / A216T, S27L / R89K / V170I / T176S / V177I / D203S, S27L / A215D, S27L / T157G / V170I / Q182E / D203V, S27L / V170I / T206D / S212Q, P20T / V23T / A24V / S27L / L90Y / T109L / S110R / I222L / V229I, R12E / P20T / V23T / A24V / S27L / T109L / S110R / D203R / I222L / V229I, R12E / P20T / V23T / A24V / S27L / L90Y / T109L / S110R / D203R / I222L / V229I, R12E / S27L / L90Y / D203R, P20T / V23T / A24V / S27L / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69S / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / A174R / H183S / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A 246Q, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / T157L / H183T / F187L / D203R / F208S / A216D / I222L / V 229I / A246Q, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216E / I222L / V229I / A246Q, S27L / V177I / A216T, A24V / S27L / I222L, S27L / D203R, P20T / V23T / A24V / S27L / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216T / I222L / V229I / A246Q, S27L / L90Y / A174G / D203V / D249L, S27L / A174G / D203L / D249L, S27L / L90A / A174G / D203R / D249L, S27L / L90Y / A174G / D203N, S27L / L90Y / A174G / D249L, S27L / L90Y / A174G / D203G / D249L, S27L / L90Y / D203L / D249L, S27L / D203L / D249L, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I, S27L / W69H / H183S / F187I / D203K / F208G, S27L / W69H / L90Y / H183S / F187I / D203K / F208G, S27L / W69H / H183S / F187I / D203R / F208G, S27L / W69H / L90Y / H183S / F187I / D203R / F208G, S27L / W69H / L90Y / A174R / H183S / D203R / F208D, and S27L / W69H / L90Y / T157A / A174R / H183S / D203R / F208D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of P20T / S27L / L90Y / I222L / V229I, V23T / A24V / S27L / L90Y / T176S / A215D, A24V / S27L / W69S / L90Y / T109L / H183T / D203R / A215D / I222L / V229I, S27L / L90Y / H183S / D203K, P20T / S27L / L90Y / T109L / D203R / F208D / I222L / V229I, S27L / L90Y / T109L / H183S, A24V / S27L / L90Y / D203K, S27L / L90Y / H183S / I222L / V229I, S27L / W69L / L90Y / H183S, S27L / L90Y / T176S / V177T, S27L / L90Y / T176S / V229I, A24V / S27L / L90Y, S27L / W69H / L90Y / A215D, A24V / S27L / L90Y / T109L / H183S / F208D, P20T / A24V / S27L / L90Y / T109L / H183S / I222L / V229I, S27L / W69L / L90Y / H183S / D203K / F208D / I222L / V229I, P20T / S27L / L90Y / D203K / F208D / I222L / V229I, A24V / S27L / L90Y / H183S / F208D / I222L / V229I, S27L / L90Y / D203R / F208D / I222L / V229I, S27L / L90Y / F208D / V229I, A24V / S27L / W69I / L90Y / H183S / D203R, S27L / W69I / L90Y / T109L / H183S / D203R / I222L / V229I, A24V / S27L / W69I / L90Y / D203K / F208D / I222L / V229I, A24V / S27L / W69L / L90Y, S27L / L90Y / D203K / I222L / V229I, A24V / S27L / L90Y / T109L / H183S / D203R / F208D, A24V / S27L / L90Y / A174R / D203K, S27L / L90Y / D203A / A215D, S27L / L90Y / I222L / V229I, S27L / W69H / L90Y / T176S / V177T / A215D, P20T / A24V / S27L / L90Y / S212Q / V229I, V23T / S27L / L90Y / T176S / V177T / D203G / A215D, S27L / L90Y / D203S / A215D / I222L / V229I, S27L / L90Y / T176S / F208D / A215D, V23T / A24V / S27L / W69H / L90Y / V177T / D203S, S27L / L90Y / V177T, S27L / L90Y / V229I, S27L / L90Y / T176S / D203S / A215D, S27L / L90Y / S212Q, S27L / L90Y / D203R / A215D / V229I, S27L / L90Y / S212Q / I222L / V229I, V23T / A24V / S27L / L90Y, P20T / S27L / L90Y / S110R / A174R / T176S / A215D / I222L / V229I, S27L / L90Y / T109L / A174R / A215D, S27L / L90Y / T109L / D203G / A215D, P20T / S27L / L90Y / S110R / D203G / A215D / I222L, S27L / L90Y / A215D, V23T / S27L / L90Y / T109L / T157A / D203R / V229I, S27L / L90Y / T176S, S27L / L90Y / D203G, V23T / A24V / S27L / L90Y / V229I, V23T / A24V / S27L / L90Y / H183T / I222L / V229I, V23T / A24V / S27L / W69L / L90Y / V177T / A215D, S27L / L90Y / A174R / H183T / D203K / S212Q, V23T / A24V / S27L / L90Y / S110R / T176S / F208D, S27L / L90Y / D203A, S27L / L90Y / F208D, P20T / S27L / L90Y / T176S / F208D / A215D, S27L / L90Y / T109L / T176S / I222L, S27L / W69L / L90Y / S212Q, S27L / L90Y / A215D / V229I, P20T / S27L / L90Y / V229I, V23T / S27L / L90Y / V229I, S27L / W69L / L90Y, V23T / A24V / S27L / L90Y / T109L / T176S / D203R / F208D, and S27L / L90Y / A215D / I222L / V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of S27L / L90Y / T176S / F187V / D203G / F208K / V229I, S27L / W69C / L90Y / T109L / S181D / H183T / D203A / F208K / I222L / V229I, S27L / W69L / L90Y / D203G / F208Q, S27L / L90Y / T109L / A174K / S181D / F208D, V23T / A24V / S27L / L90Y / A174R / S181D / H183S / F208D / A215D, P20T / S27L / W69G / L90Y / A174R / S181D / H183A / F187V / D203K / S212Q, V23T / S27L / L90Y / T176S / F208Q / A215D, A24V / S27L / L90Y / T109L / A174K / D203K / F208D / V229I, P20T / S27L / L90Y / T157A / D203K / F208V / V229I, A24V / S27L / W69L / L90Y / F187V / D203R / F208V / I222L / V229I, A24V / S27L / W69L / L90Y / T109L / A174K / D203K / F208V, S27L / L90Y / T109L / H183S / F187V, A24V / S27L / L90Y / A174K / F187V / F208D / V229I, 24V / S27L / L90Y / A174K / H183S / F187V / D203R / I222L / V229I, P20T / S27L / L90Y / H183S / D203R / F208V / V229I, S27L / L90Y / A174R / H183S / F187V / D203R, A24V / S27L / L90Y / T109L / A174R / H183S / F187V / D203R / F208D / I222L / V229I, P20T / S27L / L90Y / F208V, S27L / L90Y / H183T / D203S / F208K, P20T / V23T / S27L / L90Y / D203G / F208Q, S27L / L90Y / D203G / F208Q, V23T / A24V / S27L / L90Y / T176S / H183T / D203K / F208Q / A215D / I222L / V229I, S27L / L90Y / A174K / S181D / H183Y / D203R / F208V, V23T / A24V / S27L / L90Y / A174K / T176S / S181D / H183S, S27L / W69G / L90Y / H183Y / F187V / D203S, P20T / S27L / L90Y / T157A / S181D / H183S / D203S / A215D / I222L / V229I, S27L / W69S / L90Y / T109L / H183D / F187V / D203R / F208Q / A215D / I222L / V229I, A24V / S27L / L90Y / F208Q / A215D / V229I, V23T / S27L / L90Y / S181D / H183A / D203G / F208V / A215D / V229I, S27L / L90Y / A174R / F187V / D203G / A215D, P20T / S27L / L90Y / S181D / H183S / F187V / F208V / A215D / I222L / V229I, S27L / W69C / L90Y / A174R / H183T / F187V / A215D / I222L, S27L / L90Y / F208V / A215D / I222L / V229I, A24V / S27L / W69H / L90Y / A174R / F208I, S27L / L90Y / F208V, A24V / S27L / W69I / L90Y / A174R / H183S / F187V / D203R / F208D / I222L, A24V / S27L / L90Y / T109L / F187V / F208D, S27L / L90Y / H183S / D203R / F208V / I222L / V229I, S27L / L90Y / A174K / H183S / F208D / I222L / V229I, S27L / W69H / L90Y / A174K / H183S / D203R / F208V / V229I, S27L / L90Y / H183S / F187V / D203K, A24V / S27L / L90Y / T109L / D203K / F208V, A24V / S27L / L90Y / F187V / F208V / V229I, A24V / S27L / W69I / L90Y / A174K / H183S / F187V / D203K / F208D, S27L / L90Y / A174R / H183S / F187V / V229I, A24V / S27L / L90Y / T109L / A174K / H183S / D203K / F208D / V229I, S27L / W69H / L90Y / T109L / A174K / H183S / D203K / F208D / I222L / V229I, S27L / W69H / L90Y / H183S / F208V / I222L, A24V / S27L / L90Y / F187V / D203K / F208D, S27L / L90Y / D203R / F208V / I222L / V229I, S27L / L90Y / A174K / T176S / F208D / A215D / I222L / V229I, V23T / A24V / S27L / W69H / L90Y / T109L / A174K / D203G / F208V / V229I, V23T / S27L / L90Y / T109L / S110R / D203K / F208V / I222L / V229I, S27L / L90Y / T176S / H183Y / F187V, V23T / S27L / W69G / L90Y / T176S / V177T, V23T / S27L / W69C / L90Y / S110R / T176S / F187V / D203K, A24V / S27L / L90Y / S181D, S27L / W69I / L90Y / T157D / V177T / H183Y / F187V / I222L / V229I, A24V / S27L / L90Y / V177T / H183Y / F187V / D203G / A215D / I222L / V229I, S27L / L90Y / T176S / F208V / A215D / I222L, S27L / L90Y / A174R / H183Y / F187V / D203S / F208Q / I222L / V229I, V23T / S27L / W69C / L90Y / T176S / D203G / A215D, P20T / S27L / L90Y / T176S / S181D / H183T, V23T / S27L / L90Y / A174K / F208Q / I222L / V229I, S27L / L90Y / A174K / S181D / D203K / A215D / I222L, A24V / S27L / L90Y / T109L / A174K / S181D, A24V / S27L / W69L / L90Y / T109L / S181D / F187V / I222L / V229I, S27L / L90Y / A174K / D203G / F208Q, S27L / L90Y / S110R / D203S / F208Q, S27L / W69L / L90Y / T176S / V177T / F187V / D203S / F208D, V23T / S27L / L90Y / A174R / S181D / H183Y / F187V / D203K / F208V, A24V / S27L / L90Y / S110R / H183T / F187V / A215D, P20T / S27L / W69G / L90Y / F208D / A215D, P20T / S27L / L90Y / S110R / A174K / H183Y / F208V / A215D, S27L / L90Y / S181D / H183S / A215D / I222L / V229I, A24V / S27L / L90Y / F208Q, S27L / W69L / L90Y / T157A / A174R / H183Y / I222L / V229I, V23T / S27L / L90Y / T109L / T157D / A174K / T176S / H183Y / A215D / I222L / V229I, S27L / L90Y / T176S / V177T / F208V / A215D, V23T / A24V / S27L / W69C / L90Y / S110R / A174R / T176S / F187V / V229I, V23T / A24V / S27L / W69L / L90Y / A174R / S181D / H183Y / F187V / D203A / A215D / I222L, P20T / S27L / L90Y / T109L / T176S / V177T / F187V / I222L, S27L / L90Y / T109L / S110R / A174K / T176S, P20T / S27L / L90Y / V177T / D203A / F208Q / V229I, S27L / L90Y / T157L / T176S / F208Q / A215D, V23T / A24V / S27L / L90Y / S110R / T176S / H183A / D203A / F208Q / I222L / V229I, S27L / W69H / L90Y / T109L / S110R / H183T / D203K / F208V, A24V / S27L / W69C / L90Y / S212N / A215D, S27L / W69H / L90Y / A174R / H183T / F187V / D203R / F208V / S212Q, S27L / L90Y / A174K / F208D, 27L / L90Y / A174K, S27L / L90Y / T176S / V177T / D203R / F208V / I222L / V229I, V23T / A24V / S27L / L90Y / A174K / S181D / H183Y / F187V / I222L / V229I, P20T / S27L / W69H / L90Y / A174R / H183A / A215D, S27L / L90Y / D203S / F208K, S27L / W69H / L90Y / T109L / S110R / A174R / T176S / S181D / F187V / D203A / F208Q, S27L / W69S / L90Y / A174R / S181D / H183T / F187V / D203G / F208D, A24V / S27L / W69C / L90Y / S110R / T157A / T176S / V177T / D203A / S212Q / V229I, S27L / L90Y / S181D / A215D / V229I, P20T / S27L / W69I / L90Y / V177T / S181D / H183Y, V23T / A24V / S27L / L90Y / T109L / S110R / S181D / H183S / D203A / V229I, S27L / W69G / L90Y / T109L / A174R / A215D, S27L / W69G / L90Y, S27L / L90Y / T176S / V177T / H183A / D203K / S212Q / V229I, V23T / A24V / S27L / W69C / L90Y / T109L, V23T / S27L / L90Y / A174K / T176S, S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208V, S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / A215D / V229I, S27L / W69I / L90Y / T176S / S181D / H183S / D203A / F208V / V229I, S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203S / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183S / D203S / F208V, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / S212Q, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203G / F208V, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / A215D / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183S / F208V / V229I, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / A215D / V229I, R12T / S27L / L90Y / T176S / S181D / H183Y / D203S / F208V / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / S212Q / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203G / F208V, S27L / W69I / L90Y / S181D / H183S / D203A / F208V / S212Q / A215D, S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208Q / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / F208V / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / D203A / F208Q, R12T / S27L / L90Y / T176S / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / T176S / S181D / H183T / D203S / F208D / V229I, S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / V177T / S181D / H183T / D203S / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / S181D / H183T / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / D203A / F208D / V229I, V23T / S27L / L90Y / T176S / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / S181D / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / D203S / F208D / V229I, R12T / S27L / W69H / L90Y / V177T / S181D / H183T / F208D / V229I, S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D, V23T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / H183T / D203A / F208D / V229I, S27L / L90Y / T176S / S181D / H183T / D203S / F208D / I222L / V229I, R12T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / V177T / S181D / H183T / D203G / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / S27L / L90Y / V177T / S181D / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / V177T / S181D / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / S181D / D203A / F208D / I222L / V229I, R12T / S27L / W69H / L90Y / V177T / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / S181D / D203A / F208D / I222L, R12T / S27L / L90Y / T176S / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / T176S / H183T / F208D / I222L / V229I, R12T / P20T / S27L / L90Y / H183S / I222L / V229I, R12T / S27L / L90Y / D203A / F208D, R12T / S27L / L90Y / A174R / T176S / H183Y / F208Q / I222L / V229I, R12T / V23T / A24V / S27L / W69S / L90Y / A174R / T176S / H183T / D203A / V229I, R12T / A24V / S27L / L90Y / T109L / H183Y / D203R / A215D / I222L / V229I, R12T / P20T / S27L / W69L / L90Y / S181D / D203R / F208V / I222L / V229I, R12T / P20T / S27L / L90Y / A174K / T176S / D203G / F208Q / A215D / I222L / V229I, R12T / S27L / L90Y / H183S / A215D / I222L, R12T / S27L / L90Y / T176S / V177T / D203S / A215D, R12T / V23T / S27L / L90Y / H183T / D203S / F208K / A215D, R12T / A24V / S27L / W69S / L90Y / T176S / D203R / I222L / V229I, R12T / V23T / S27L / L90Y / F208Q / A215D / I222L / V229I, R12T / A24V / S27L / L90Y / V177T / A215D / I222L / V229I, R12T / S27L / L90Y / A174R, R12T / S27L / L90Y / A215D / V229I, R12T / P20T / S27L / L90Y / D203K / A215D / V229I, R12T / A24V / S27L / L90Y / T176S, R12T / P20T / S27L / W69H / L90Y / T109L / S110R / A174R / H183Y / A215D, R12T / S27L / L90Y / T176S / A215D / I222L, R12T / S27L / L90Y, R12T / P20T / S27L / L90Y / H183A / F208K, R12T / V23T / A24V / S27L / L90Y / T176S / V229I, R12T / S27L / W69G / L90Y / D203K, R12T / A24V / S27L / L90Y / T109L / A215D, R12T / V23T / S27L / L90Y / T176S / H183Y / V229I, R12T / S27L / W69F / L90Y / T176S / V177T / S181D / H183T / F208D, and R12T / S27L / W69F / L90Y / S181D / D203A / F208D / I222L / V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of V23T / A24V / S27L / L90Y / A174K / T176S / F208Q / A215D / I222L / V229I / S248R, R12T / S27L / L90Y / E173N / A174R / A215D, V23T / A24V / S27L / L90Y / N204G / F208V / S212Q / P213D / I222L / V229I, A24V / S27L / L90Y / D203R / F208V / P213D / V229I, R12T / P20T / S27L / L90Y / A174R / T176S / S181D / H183Y / F187V / D203G / F208Q / S248R, R12T / S27L / L90Y / H183D / D203G / F208K / A215D / I222L / V229I / S248R, S27L / L90Y / D203S / N204G / F208V / I222L / V229I, R12T / S27L / L90Y / H183T / N204G / A215D, R12T / P20T / S27L / L90Y / D203R / F208V / P213D / I222L / V229I, P20T / V23T / A24V / S27L / W69C / L90Y / T109L / F187V / D203A / N204G / I222L / V229I, S27L / L90Y / E173T / A174K / S181D / H183Y / A215D / I222L / V229I / S248R, V23T / S27L / W69H / L90Y / S212Q / P213D / I222L / V229I, R12T / S27L / L90Y / D203K / N204G, P20T / S27L / L90Y / S181D / H183A / F208Q / I222L / V229I / S248R, A24V / S27L / L90Y / T109L / A174R / H183A / F208Q / A215D / V229I / S248R, S27L / L90Y / F208K / A215D / V229I / S248R, V23T / A24V / S27L / L90Y / E173T / A174R / S181D / H183S / F208Q / A215D / I222L / V229I, A24V / S27L / W69I / L90Y / A174K / T176S / D203S / F208K / P213D / I222L / V229I, R12T / S27L / L90Y / T109L / E173T / A174R / S181D / H183Y, R12T / A24V / S27L / L90Y / A174R / T176S / I222L / V229I / S248R, R12T / S27L / W69I / L90Y / T176S / V177T / P213D / I222L / V229I, S27L / L90Y / E173V / A174K / D203A / F208Q / A215D, V23T / S27L / L90Y / T176S / D203S / N204G / A215D, S27L / L90Y / F208K / A215D / S248R, A24V / S27L / W69C / L90Y / T109L / V177T / D203K / S212Q / P213D, A24V / S27L / L90Y / E173N / A174K / S181D / A215D / I222L / V229I, R12T / P20T / A24V / S27L / W69I / L90Y / H183Y / D203S / F208Q / S248R, V23T / A24V / S27L / L90Y / E173W / A174K / S181D / F187V / F208Q / S248R, S27L / L90Y / F208D / S248R, S27L / L90Y / T109L / S110R / S181D / N204G / F208K / A215D / S248R, P20T / S27L / W69L / L90Y / A174K / S181D / H183T / N204G / F208K / A215D / S248R, S27L / L90Y / A174K / T176S / F208D / I222L / V229I / S248R, S27L / L90Y / E173W / A174R / S212Q / P213D / I222L / V229I, S27L / L90Y / D203R / P213D, V23T / A24V / S27L / L90Y / F187V / F208Q / P213D / I222L / V229I, A24V / S27L / W69H / L90Y / T157A / E173N / S181D / F187V / F208K / I222L / V229I, S27L / L90Y / S110R / E173V / A174K / H183T / F187V / D203G / F208Q / A215D / I222L / V229I, V23T / A24V / S27L / W69S / L90Y / F208V / I222L / V229I / S248R, S27L / L90Y / A174K / S181D / D203K / N204G / F208Q, S27L / L90Y / V177T / S212Q / P213D / V229I, S27L / L90Y / T109L / H183Y / D203S / N204G / F208K / I222L / V229I, S27L / L90Y / T109L / S110R / S181D / H183T / D203K / F208D / P213D / S248R, R12T / V23T / A24V / S27L / L90Y / D203S / N204G / F208Q / A215D, S27L / W69I / L90Y / T109L / S212Q / P213D / I222L / S248R, S27L / L90Y / E173V / A174R / H183A / A215D / S248R, S27L / L90Y / E173V / A174R / F208K / V229I, S27L / L90Y / S110R / N204G / F208Q, S27L / L90Y / T176S / F187V / S212Q / S248R, S27L / L90Y / E173N / A174R, S27L / L90Y / D203R / N204G / F208V / A215D / I222L / V229I, S27L / L90Y / E173N / A174R / F187V, R12T / A24V / S27L / W69S / L90Y / T176S / S181D / D203G / N204G / S212Q / P213D / I222L / V229I, S27L / L90Y / T109L / S110R / A174R / F187V / D203G / F208V / S248R, S27L / L90Y / S110R / H183S / D203A / F208Q / S248R, R12T / A24V / S27L / L90Y / E173V / A174K / D203R / N204G / F208K / P213D / I222L / V229I, S27L / L90Y / T176S / D203G / N204G / F208D, R12T / V23T / S27L / W69C / L90Y / D203K / A215D / I222L / V229I / S248R, S27L / L90Y / T176S / V177T / N204G / A215D, S27L / L90Y / T176S / D203S / F208D / P213D / I222L / V229I, P20T / S27L / L90Y / T176S / P213D, R12T / S27L / L90Y / E173P / S181D / D203A / F208Q / I222L / V229I / S248R, V23T / A24V / S27L / L90Y / E173V / A174R / V177T / I222L / V229I, V23T / S27L / W69L / L90Y / F208Q / S248R, S27L / L90Y / A174K / F208Q / A215D / S248R, A24V / S27L / L90Y / D203R / F208K / A215D / I222L / V229I / S248R, S27L / W69C / L90Y / S110R / D203G / N204G / S212Q / V229I / S248R, V23T / S27L / L90Y / A174R / S181D / H183Y / F187V / F208D / P213D / S248R, R12T / V23T / S27L / W69S / L90Y / T109L / E173N / A174R / H183Y / A215D / S248R, R12T / S27L / W69L / L90Y / E173T / A174R / S181D / H183Y / A215D / V229I, R12T / S27L / L90Y / A215D / I222L / S248R, V23T / A24V / S27L / L90Y / A174R / T176S / H183A / D203A / N204G / A215D, S27L / L90Y / E173N / S181D / H183A / I222L / V229I / S248R, S27L / L90Y / V229I / S248R, S27L / L90Y / P213D / I222L, A24V / S27L / W69S / L90Y / E173N / A174R / F208D / A215D / V229I, V23T / A24V / S27L / L90Y / S110R / T176S / D203G / N204G / F208K / A215D / I222L / V229I, S27L / L90Y / N204G / F208D / I222L / V229I, R12T / A24V / S27L / L90Y / D203A / N204G / F208Q / A215D / I222L / V229I, V23T / A24V / S27L / L90Y / T109L / E173T / H183T, S27L / L90Y / E173T / D203K / F208D / I222L / V229I, R12T / V23T / A24V / S27L / L90Y / E173T / A174R / S181D / H183Y / F187V / D203R / F208D / V229I / S248R, S27L / L90Y / T109L / E173W / A174R / S181D / H183Y / D203S / F208D / A215D, A24V / S27L / W69L / L90Y / D203N / F208Q / A215D / V229I / S248R, P20T / S27L / W69I / L90Y / E173T / A174K / T176S / F187V / N204G / F208V / A215D / V229I, P20T / S27L / W69S / L90Y / F187V / S212Q / P213D / I222L / V229I, V23T / A24V / S27L / W69H / L90Y / T109L / S110R / T176S / P213D / I222L / V229I / S248R, R12T / V23T / S27L / L90Y / S212Q / S248R, S27L / L90Y / P213D, S27L / W69G / L90Y / A174R / S212Q / P213D, V23T / S27L / L90Y / D203A / N204G / A215D / I222L / V229I / S248R, S27L / L90Y / A174K / T176S / F187V / D203G / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / E173N / A174K / D203S / F208Q, S27L / W69I / L90Y / T157L / E173N / A215D, A24V / S27L / L90Y / S110R / S181D / H183S / D203S / N204G / A215D, R12T / P20T / S27L / L90Y / S212Q / P213D, R12T / P20T / S27L / L90Y / T176S / V177T / I222L / V229I / S248R, S27L / L90Y / T109L / H183S / D203K / F208D / V229I / S248R, R12T / S27L / L90Y / T109L / A174R / S181D / F208K / I222L / S248R, S27L / W69C / L90Y / I222L / V229I / S248R, P20T / S27L / L90Y / A174K / T176S / H183S / D203K / N204G, S27L / W69C / L90Y / T176S / S212Q / P213D / V229I / S248R, A24V / S27L / W69G / L90Y / S110R / D203G / N204G / A215D / I222L / V229I, S27L / L90Y / A174R / T176S / D203G / F208Q / A215D / S218R / I222L / V229I / S248R, S27L / L90Y / S110R / E173N / F187V / F208Q / A215D / I222L / V229I / S248R, P20T / S27L / L90Y / V177T / F187V / D203S / A215D / I222L / S223N / V229I, S27L / L90Y / T109L / S181D / H183S / D203R / N204G / A215D / I222L / V229I / S248R, V23T / A24V / S27L / L90Y / E173N / A174R / D203A / A215D / I222L, P20T / S27L / W69L / L90Y / F187V / F208Q / S248R, P20T / S27L / W69H / L90Y / E173T / A174K / S181D / A215D, V23T / S27L / L90Y / E173V / A174R / S181D / F187V / D203R / A215D / I222L / V229I, S27L / W69H / L90Y / T109L / S181D / D203S / N204G / A215D, S27L / L90Y / N204G / F208D / I222L, S27L / L90Y / T109L / S110R / S181D / H183A / D203G / N204G / F208K / I222L, 12T / A24V / S27L / L90Y / E173P / A174K / A215D, A24V / S27L / W69H / L90Y / T109L / S110R / A174K / T176S / D203S / S212Q / P213D / I222L / V229I, A24V / S27L / L90Y / S212Q / P213D / I222L / V229I, R12T / P20T / S27L / L90Y / T176S / V177T / P213D / I222L / V229I, S27L / L90Y / E173P / S181D / D203K / N204G / S212Q / P213D / I222L / V229I, S27L / W69I / L90Y / S110R / H183S / D203R / N204G / A215D, A24V / S27L / W69C / L90Y / T109L / S110R / T176S / D203A / A215D / I222L / S248R, V23T / S27L / L90Y / H183Y / A215D / S248R, S27L / L90Y / T176S / D203S / P213D, S27L / L90Y / S181D / H183A / P213D / V229I, S27L / L90Y / I222L / V229I / S248R, R12T / S27L / L90Y / A174K / P213D / I222L / V229I, R12T / T16A / V23T / A24V / S27L / L90Y / T176S / V177T / A215D / I222L / V229I / S248R, S27L / W69H / L90Y / T109L / D203S / N204G / P213D, V23T / A24V / S27L / L90Y / V177T / D203S / N204G / A215D / I222L / V229I, S27L / L90Y / A174K / T176S / F208Q / A215D / I222L / S248R, A24V / S27L / L90Y / S181D / H183T / D203K / N204G / F208Q / A215D / I222L / V229I, A24V / S27L / L90Y / V177T / D203K / A215D / V229I / S248R, V23T / A24V / S27L / L90Y / D203R / N204G / A215D / S248R, R12T / S27L / L90Y / T109L / S181D / H183T / N204G / F208Q / S212Q / P213D / I222L / V229I / S248R, A24V / S27L / L90Y / F208Q / I222L / V229I / S248R, S27L / L90Y / A174R / T176S / H183T / P213D / V229I, V23T / A24V / S27L / L90Y / E173W / A174K / D203S / F208D, A24V / S27L / W69L / L90Y / S181D / H183Y / F187V / F208V / A215D / S248R, R12T / S27L / L90Y / E173W / A174K, V23T / A24V / S27L / L90Y / D203G / N204G / A215D / I222L / V229I, S27L / L90Y / D203A / N204G / F208V / A215D, V23T / S27L / L90Y / T109L / S110R / A174K / T176S / N204G / F208Q / I222L / V229I, S27L / L90Y / E173P / A174K / D203G / F208V / I222L / V229I, A24V / S27L / W69H / L90Y / S110R / D203R / N204G / F208K, P20T / S27L / L90Y / E173N / A174K / H183Y / D203K / F208Q / I222L / V229I, P8L / A24V / S27L / L90Y / T109L / S110R / A215D / I222L / V229I, P20T / S27L / L90Y / S110R / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / S110R / E173N / A174K / S181D / F187V / F208Q / I222L / V229I, A24V / S27L / W69L / L90Y / P213D, S27L / L90Y / S218G / I222L / V229I / S248R, A24V / S27L / L90Y / E173T / A174R / F208V / V229I, S27L / W69I / L90Y / S181D / F187V / A215D / I222L / V229I / S248R, S27L / W69G / L90Y / E173P / A174R / S212Q / V229I, P20T / S27L / L90Y / E173W / A174R / S181D / F187V / D203R / N204G / P213D / I222L / V229I, S27L / L90Y / A174R / T176S / H183D / F187V / D203R / N204G / F208V / A215D / V229I, R12T / P20T / S27L / L90Y / E173P / S181D / D203K / N204G / F208K, P20T / S27L / L90Y / T109L / D203G / I222L / V229I / S248R, S27L / L90Y / E173N / A174K / F208K, R12T / V23T / S27L / L90Y / E173V / A174R / F208D / S212Q / I222L / V229I, V23T / A24V / S27L / L90Y / S110R / E173N / A174K / H183T / D203R / N204G / F208Q / I222L / V229I, S27L / W69L / L90Y / T109L / E173V / A174K / D203G / A215D / I222L / V229I, A24V / S27L / L90Y / T176S / V177T / H183Y / D203K / S212Q / P213D / I222L / V229I, P20T / S27L / L90Y / V177T / A215D / I222L / S248R, V23T / A24V / S27L / L90Y / P213D / V229I, S27L / L90Y / D203K / N204G / V229I, A24V / S27L / W69I / L90Y / E173W / S181D / A215D, S27L / W69S / L90Y / A174R / D203S / N204G / F208Q / A215D / V229I / S248R, S27L / L90Y / T176S / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / E173N / A174K / I222L / V229I, S27L / L90Y / A215D / V229I / S248R, P20T / S27L / L90Y / A174R / T176S / N204G / F208Q, S27L / L90Y / E173V / A174K / F187V / D203S / F208Q / I222L / V229I, V23T / S27L / L90Y / V177T / F187V / S212Q / P213D, S27L / L90Y / A174R / S181D / F208V / A215D / I222L / V229I / S248R, P20T / S27L / L90Y / S181D / H183A / S212Q / I222L / V229I / S248R, S27L / L90Y / D203G / N204G, V23T / S27L / L90Y / P112Q / E173T / A174K / H183Y / D203R / F208D / A215D / V229I / S248R, R12T / S27L / L90Y / H183Y / S212Q / I222L / V229I / S248R, S27L / W69S / L90Y / H183Y / A215D / I222L / V229I / S248R, S27L / L90Y / H183Y / A215D / S248R, V23T / S27L / L90Y / T109L / S110R / D203K / N204G / F208D / I222L / V229I, R12T / A24V / S27L / L90Y / S110R / S181D / H183Y / P213D, S27L / L90Y / S248R, R12T / P20T / S27L / W69H / L90Y / T109L / S110R / P213D / V229I, S27L / W69G / L90Y / T109L / E173T / A174K / A215D / V229I, A24V / S27L / L90Y / A174R / P213D, V23T / A24V / S27L / L90Y / A174K / T176S / P213D / V229I, A24V / S27L / L90Y / A174K / A215D / I222L / V229I / S248R, R12T / S27L / W69G / L90Y / E173N / A174K / D203K, V23T / A24V / S27L / L90Y / E173W / A174K, P20T / S27L / L90Y / D203S / N204G / A215D / I222L / V229I, R12T / S27L / L90Y / A215D / S248R, R12T / P20T / S27L / W69C / L90Y / T109L / E173V / A174R / D203A, A24V / S27L / W69C / L90Y / V177T / F208K / S212Q / P213D / S248R, S27L / L90Y / T109L / S110R / E173V / A174K / A215D, R12T / P20T / S27L / L90Y / E173T / A174K / F187V / D203A / N204G / A215D / I222L / V229I, S27L / L90Y / E173N / S181D / F187V / F208K / S248R, R12T / S27L / L90Y / T176S / V177T / A215D / S248R, A24V / S27L / L90Y / S110R / E173P / A174R / H183Y / A215D / S248R, R12T / P20T / S27L / L90Y / D203S / N204G / F208V / A215D, S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / P213D / V229I, S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / S181D / H183S / D203S / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183S / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183S / D203S / F208V / P213D, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D, R12T / S27L / L90Y / S181D / H183Y / D203A / F208V / P213D / V229I, R12T / S27L / L90Y / T176S / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D / A215D, S27L / W69I / L90Y / S181D / H183T / D203A / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / P213D / A215D, R12T / S27L / L90Y / V177T / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / P213D / A215D, S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / S212Q / P213D, S27L / W69I / L90Y / T176S / V177T / S181D / H183S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208Q / P213D / V229I, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / D203A / F208V / P213D / V229I, S27L / W69I / L90Y / S181D / H183S / F208V / S212Q / P213D / V229I, S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / S212Q / P213D, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / D203A / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / V177T / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183S / F208V / P213D / A215D, S27L / W69I / L90Y / T176S / S181D / H183Y / F208Q / P213D / A215D, R12T / S27L / W69I / L90Y / V177T / S181D / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183S / F208V / S212Q / P213D, and R12T / S27L / W69I / L90Y / S181D / H183A / D203A / F208Q / P213D. In another aspect, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 20, 23, 24, 69, 109, 110, 157, 174, 176, 177, 181, 183, 187, 203, 204, 208, 212, 213, 215, 216, 222, 229 and 246, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 176, 177, 181, 204, 212, 213 and 215. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 176, 177, 181, 204, 212, 213 and 215. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 20, 23, 24, 69, 109, 110, 157, 174, 176, 177, 181, 183, 187, 203, 204, 208, 212, 213, 215, 216, 222, 229 and 246. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase, said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, P20T, V23T, A24V, W69H, W69I, W69L, W69S, T109L, S110R, T157A, T157L, A174R, H183S, H183T, F187I, F187L, D203K, D203R, F208D, F208G, F208S, F208T, A216D, A216E, A216T, I222L, V229I, and A246Q. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of W69F, H183Y, D203A, D203S, F208Q, and F208V. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of R12T, T176S, V177T, S181D, N204G, S212Q, P213D, and A215D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, T109L, S110R, T157A, T157L, A174R, T176S, V177T, S181D, H183S, H183T, H183Y, F187I, F187L, D203A, D203K, D203R, D203S, N204G, F208D, F208G, F208Q, F208S, F208T, F208V, S212Q, P213D, A215D, A216D, A216E, A216T, I222L, V229I, and A246Q. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, T157D, T157E, T157G, T157Q, T157S, A174G, D203E, D203G, D203L, D203N, D203V, D203Y, A216I, A246E, and A246N. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, V177I, V177W, S181A, S181Q, S181T, N204S, S212E, S212H, S212I, P213A, A215K, and A215S. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, T157D, T157E, T157G, T157Q, T157S, A174G, V177I, V177W, S181A, S181Q, S181T, D203E, D203G, D203L, D203N, D203V, D203Y, N204S, S212E, S212H, S212I, P213A, A215K, A215S, A216I, A246E and A246N. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of A14V, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, H156N, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, Q182D, Q182E, Q182S, T206D, T206S, A209E, N211Q, N214D, I217M, S248C, S248R, and D249L. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, L90Y, R12E, R12F, R12L, R12M, R12Q, R12S, R12T, A14V, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, T109L, S110R, H156N, T157A, T157D, T157E, T157G, T157L, T157Q, T157S, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, A174G, A174R, T176S, V177I, V177T, V177W, S181A, S181D, S181Q, S181T, Q182D, Q182E, Q182S, H183S, H183T, H183Y, F187I, F187L, D203A, D203E, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, N204S, T206D, T206S, F208D, F208G, F208Q, F208S, F208T, F208V, A209E, N211Q, S212E, S212H, S212I, S212Q, P213A, P213D, N214D, A215D, A215K, A215S, A216D, A216E, A216I, A216T, I217M, I222L, V229I, A246E, A246N, A246Q, S248C, S248R, and D249L. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, P20T, V23T, A24V, W69H, A174R, H183T, F208D, I222L, and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, W69F, D203A and D203S. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, T176S, V177T, and S181D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203S, F208D, I222L, and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of W69I, W69L, H183S, H183Y, D203G, D203R, F208Q, and F208V. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of N204G, S212Q, P213D, and A215D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, A174R, T176S, V177T, S181D, H183S, H183T, H183Y, D203A, D203G, D203R, D203S, N204G, F208D, F208Q, F208V, S212Q, P213D, A215D, I222L, and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, seventeen of said posiƟons, eighteen of said posiƟons, nineteen of said posiƟons or twenty of said posiƟons. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y, P20T / V23T / A24V / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69S / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / A174R / H183S / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A246Q, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / T157L / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A246Q, P20T / V23T / A24V / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216E / I222L / V229I / A246Q, P20T / V23T / A24V / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216T / I222L / V229I / A246Q, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I, W69H / H183S / F187I / D203K / F208G, W69H / L90Y / H183S / F187I / D203K / F208G, W69H / H183S / F187I / D203R / F208G, W69H / L90Y / H183S / F187I / D203R / F208G, W69H / L90Y / A174R / H183S / D203R / F208D, and W69H / L90Y / T157A / A174R / H183S / D203R / F208D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12T / L90Y / A174R / T176S / H183Y / F208Q / I222L / V229I, R12T / P20T / W69L / L90Y / S181D / D203R / F208V / I222L / V229I, R12T / L90Y / A174R / T176S / H183T / F208D / I222L / V229I, R12T / W69H / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / W69H / L90Y / S181D / D203A / F208D / I222L / V229I, R12T / W69F / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / W69F / L90Y / S181D / D203A / F208D / I222L / V229I, V23T / A24V / L90Y / N204G / F208V / S212Q / P213D / I222L / V229I, R12T / P20T / L90Y / D203S / N204G / F208V / A215D, R12T / W69I / L90Y / V177T / S181D / H183S / F208V / P213D, R12T / W69I / L90Y / S181D / H183Y / F208V / P213D / A215D, R12T / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D, R12T / W69I / L90Y / V177T / H183Y / D203A / F208V / P213D, and R12T / W69I / L90Y / V177T / S181D / D203A / F208V / P213D. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / T176S. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of D203A, S181D, D203R, R12T, V177T, L90Y / T176S, T176S, A215D, N204G, and D203S. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / A246E, A174G / D203A / A216E, and L90Y / A174G. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12F / L90Y, R12T / L90A / T176S / S212E, and R12Q / T176S. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, T176S / V177T / A215D / A246E, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, and T176S / D203A / A216I. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12F / L90Y, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / A246E, A174G / D203A / A216E, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, R12Q / T176S, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, L90Y / A174G, T176S / D203A / A216I, R12T / L90A / T176S / S212E, and T176S / V177T / A215D / A246E. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12E / V170I / V177T / S212H, R12F / V170L / T176S / V177I / S181D, R12F / T157L / V170I / T176S / D203A / S212E, L90F / V170I / T176S / V177I / Q182D / A216T, L90Y / T157Q / V170I / S181Q / Q182D, R12T / L90F / F92G / V170I / A215D, R12T / V170L / S181T / Q182D, L90Y / A174G / T176S / V177I / Q182E, L90Y / V170I / T176S / V177I / Q182D / A246E, R12E / R89K / L90F / V170I / T176S, R12M / V170I / T176S / V177I / S212Q, R12M / V170L / A174G / S181D, R12L / F92G / T157E / V170L / S181T / Q182D / T206D / S212Q / A246Q, V170I / S181Q / Q182E / D203Y / A215D, R12M / R89K / L90Y / V170L / T176S / V177I / T206D / S212H, R12L / R89K / L90Y / V170I / V177I / S181Q / Q182D, R12S / L90Y / A246N / S248R, R12S / Q182E / D203S / A216T, R12T / V170I / D203A / S212H, T157Q / V170L / T176S / V177T / A215D / A216T, R12E / E173A / V177T / A215D / A216T, F92G / T157E / A174G / T176S / V177W / S181D / A215D / A216T, E173T / S181D / D203N, R12S / V170L / A174G / S181D, E173P / S181T / D203R / A216T / A246E / S248C, R12T / A174G / S181T / Q182D / A246E / S248R, R12S / A215D / A216T / S248C, R12M / T157S / V170L / T176S / V177T / S181D / Q182D, R12T / Q182D / A215D / A216T, V170L / A174G / T176S / Q182D / S212Q / A246E, R12S / V170L / A215D / A216T / A246E, R12T / L90A / E173P / A174G / S181D / Q182E, R89M / T157G / S181T / Q182E, R89K / V170I / T176S / V177I / D203S, L90F / F92D / V170L / T176S / D203S / T206D / S212H, V170I / E173T / S181T / Q182D / D203G / A215D / A246E, V170L / T176S / Q182D, T157Q / V170I / V177T / T206D / S212Q / D249L, R12E / T157E / V170I / A174G / T176S / Q182D, R12F / T157Q / V170I / D203G / S212Q / D249L, R89K / L90F / V170I / V177T, R12T / E173A / A174G / S181T / Q182D, T157A / V170L / V177T, E173P / S181D, E173R / A174G / A209E / A215D / A246Q, L90F / V170I / Q182E / A215D, F92G / T157L / V170I / T176S / S181Q / Q182E / S212H, R12T / E173T / A174G / S181D / Q182D, R12T / R89K / L90Y / T157A / V170I / A174G / T176S, L90Y / V170I / V177I / A215D / A216T, V170I / V177I / T206D / A215D, L90F / T157E / V170I / T176S / V177I / S212H, R12T / V170I / T176S / V177T, V170I / T206D / S212Q, R12E / L90F / V170I / T176S / S181T / Q182D, V170I / T176S / V177T / A246E, V170I / T176S / D203K, L90F / F92G / V170I / S181Q / Q182D / T206D / A215D, T157A / D203V / A209E / A215D, T157E / V170L / V177I / Q182E / A215D / A246N, T157Q / V170I / S181D / S212H, V170I / T176S / V177I / Q182D / N204G / T206D, T157G / V170I / S181T / S212Q, L90Y / E173R / Q182D / N204G / T206D / S212H, R89K / L90F / T176S / V177I, T157S / E173T / A174G / S181T / Q182E / A215D / A246N, L90F / F92G / V170I / T176S / V177T, E173N / S181D / S212E, R12S / E173P / S181D, R12E / T157L / E173N / A174G / S181D / Q182D, R12T / V170I, R12T / T157Q / V170I / A174G / T176S / A215D / A216T, L90Y / E173N / S181T / Q182E, L90F / V170L / T176S / V177T / A215D, R12T / R89K / L90F / V170I / S181D / Q182D / D249L, R12T / L90A / E173N / A209E, V170I / A215D / A216T, V170L / V177T, V170L / V177T / T206D / A215D, V170I / E173T / S181D, L90A / T157S / V170L / V177I / T206D / S212Q, V170L / T176S / V177I / S181D / D249L, T157A / A174G / T176S / S181D / Q182D / A215D / A216T, L90F / F92G / T157Q / V170I / V177W / A209E / A215D / A216T, V170I / A174G / T176S / Q182E / T206D / A216T, L90Y / F92G / T157G / V170I / T176S / V177I / D203A / S212Q / A246E, T157G / A209E / A215D / A246Q, R89K / L90Y / T157S / A174G / S181T / Q182D, V170I / V177T, V170L / T176S / A215D, E173N / S181D / D203G / A209E / A215D / A216T, T157Q / V170I / V177T / A246E, R12T / L90F / E173R / S181Q / Q182E / A209E, F92G / T157Q / E173R / A174G / S181D / Q182D / A215D / A216T / S248R / D249L, R12T / R89K / L90Y / V170I, T157G / V170I / Q182E / D203V, F92G / T157Q / V170I, T206D, E173T / A174G / Q182D / D203G / A216T, L90Y / T157S / V170L, R12T / T157L / V170L / V177T / D203S / A215D, R12E / V170I / A174G / Q182D / D203R / A215D / A216T, R12S / R89K / T157L / V170I / T176S / S181D / Q182D / D203G / A216T, R12S / R89K / L90Y / T176S / V177I / Q182D / D203A, R12T / R89K / V170I / V177T, R12E / E173P / S181T / Q182D / A215D / A216T, R12S / L90Y / S181T / Q182D / D203R, R12E / L90Y / T157L / E173A / A215D / A216T / A246E, R12E / V170L / D203R / A215D / A246E, R12E / L90Y / V170I / E173T / Q182D / A215D / A246E, R12T / L90Y / V170L / A215D, R12E / V170I / V177T / D203R, R12E / V170L / T176S / A215D, R12E / F92G / V170I / A174G / T176S / D203R / A216T, R12E / V170I / E173P / S181T / D203R, R12E / L90Y / V170I / A174G / V177I / S181D / A215D / A216T / A246E / S248R, R12E / V170L / D203R / A246E, V170L / V177T / A246E, R12T / L90Y / V170I / D203G, R89K / L90Y / V177I / S181D / D203G / A215D / A216T, R12T / T157S / E173P / A174G / S181D / D203R / A246E / S248R, R12E / L90Y / V170L / A174G / S181T / Q182D, R12E / S181T / Q182D / D203R / A215D, R12E / L90F / F92G / E173A / S181D / D203R, R12S / V170L / T176S / S181D, R12S / V170I / V177T / D203R, R12T / L90Y / V170L / D203A, L90Y / V170I / T176S / V177T / S181D / D203R / A246E / S248R, R12S / V170I / T176S / V177T / S181D / Q182D / A246E, R12S / V170I / Q182D / A215D / A216T, R12T / L90Y / V170I / V177T, L90Y / E173P / A174G / S181D / D203G, R12S / R89K / L90A / V170I / A174G / T176S / S181D / Q182D, R12T / L90F / E173A / A174G / S181D / A216T / D249L, L90Y / V170I / T176S / V177I / S181D / Q182D / A246E / S248C, R12T / R89K / L90Y / T157A, L90Y / H156N / T157L / T176S / N204G / T206S / A215D, V170I / T176S / V177T / D203R / A246E / S248R, R12E / V170L / S181D / A215D, R12T / T157E / V170L / T176S / S181T / D203R / A215D / A246E, R12T / L90Y / V170L / V177I / A216T, R12E / L90Y / T157A / E173A / S181T / Q182D / A215D / A216T, L90Y / V170I / V177T / A216T, R12E / N87K / R89K / V170I / T176S / V177I / D203R / A215D / A216T / D249L, R12E / V170I / T176S / V177I / S181D / Q182D, L90Y / F92G / T157A / V170I / D203G / A216T / A246E / S248C, R12T / L90Y / V170L / T176S / V177T / D203R / A216T, R12T / L90F / V170I / S181D, R12T / L90Y / V170L / T176S / V177T / D203R / A246E, F92G / V170I / V177I / S181D, R12E / F92G / V170I / T176S / V177T / A215D / A216T / A246E, R12T / V170I / T176S / V177T / D203R, R12E / L90Y / E173P / A174G / S181T / Q182D, R12E / L90Y / V170I / T176S / S181T / D203R / A216T / A246E, R12T / D203R / D249L, R12E / L90Y / T157L / V170L / T176S / V177T / S181D / Q182D / D203R / A246E, R12S / V170L / T176S / V177I, L90Y / V170L / T176S / V177I, R12E / L90F / F92G / V170L / V177T / D203R / A216T, L90Y / V170L / V177T / D203G / A216T / A246E, V170I / A174G / S181D / D203R / A246E / S248R, R12E / T157E / T176S / Q182D / A215D / A216T, S181A / Q182S / D203S, R12S / V170I / S181D / D203R / A215D / A216T, R12E / V170L / T176S / S181D / Q182D / A216T, L90F / F92G / V170I / V177T / D203R / A216T, R12S / E173P / S181T / D203R / A215D / A216T / A246E, R12E / R89K / L90Y / D203R, R12E / F92G / A215D / A216T / A246E / S248R, R12S / T157S / E173T / A174G / D203R / A215D, R12E / L90A / E173P / A174G / S181T / Q182D / A215D / A216T, R89I / L90Y / H156N / T157L / V177I, L90Y / D249L, R89I / A174G / T176S / A215K, F92G / T157A / E173P / A174G / S181D / A216T, R12E / F92G / T176S / V177I, R12T / V170L / T176S / V177T / D203R / A216T, L90Y / F92Q / N204G / N211Q / A215S, L90Y / F92G / Q182S / N211Q / S212I / A216E / I217M, T176S / N211Q, L90F / D203R / D249L, R12T / L90Y / E173P / A174G / A246E, R89K / V170L / T176S / V177I / A215D, R89I / L90Y / T176S / S181A / Q182S / N204S / P213A / N214D, R12L / R89K / L90Y / V170L / T176S / V177I / N204G / A215D, R89K / V170L / T176S, T157S / V170I / V177T / S181T / Q182D / D203S / S212E / A246Q / S248C, D203G / A216E / I217M, R12T / R89K / T157G / D203N / S212E / A246E, E173R / A174G / Q182D / D203R / A215D / A216T / A246N / S248C, R12S / L90Y / V170I / E173A / A174G / S181T / Q182D / S248C / D249L, R12T / R89K / L90A / E173R / A174G / S181T / Q182D / A215D / A216T, R12T / V177T / D203V / A215D / A246N / S248C, R12T / V170L / V177T / S181T / A216T, R12T / R89K / L90Y / Q182D / A246E / S248C, R12E / F92G / V170L / V177T / A215D / S248C, R12T / L90A / D203Y / A209E / S212Q / A246Q, R12M / E173P / A174G / S181D / A209E / S212Q / A246E / S248R, R12T / R89K / L90Y / S212H / A215D / A246Q / S248C, R12T / R89K / L90A / A174G / T176S / S181T / Q182D / A215D / A246Q, R89K / L90F / A174G / S181D / S212E / A246E / S248C, R12M / E173R / S181T / Q182E / S212Q / A216T, R12L / R89K / L90Y / T157D / E173P / A174G / S181Q / Q182D / S212H, R12T / R89K / L90A / A215D / A216T / D249L, R12T / L90A / A246E / S248C, E173R / Q182D / A215D / A216T / S248R / D249L, R89K / L90Y / T176S / V177W / D203Y / A216T, R12M / V170I / A174G / T176S / S181T / T206D / A246Q / S248C, R12F / V170L / V177I / S181D / Q182E / S212E / A246E, R12T / R89K / L90F / V170L / T176S / S181Q / A216T, R12E / L90A / E173R / S181Q / Q182E / A209E / S212Q / A246N / S248C, R12T / T157Q / V170L / T176S / V177T / T206D / A209E / A216T, R12T / R89K, R12T / T157Q / V170I / E173N / S181T / Q182D / A215D / A216T / A246Q / S248C, R89K / L90Y / T206D / A209E / S212H / S248C, R12T / R89K / L90Y / A216T, R89K / T176S / V177I / S181Q / T206D / A209E / A215D / A216T / A246E, V170L / E173N / V177T / Q182D / T206D / A216T / A246Q, R12T / Q182E / D203V / A216T / A246E / S248C, L90Y / V170I / V177T / D203Y / S212E / A246E / S248C, R89K / L90Y / V170L / A174G / T176S / S181Q / S212E / A215D / A216T / A246E, T157Q / V170L / T176S / Q182D / A209E / A216T / A246Q, R12E / T157Q / E173R / A174G / Q182E / D203L / N204G / S212Q / A246E / S248C, R12M / A14V / V170I / V177T / S212H / A246E / S248C, R12L / L90A / D91N / F92G / A215D / A216T, R12T / V170L / V177T / A216T, L90A / S212Q / A246E / S248C, R12L / R89K / L90Y / V170I / V177I / D203R, R12T / S181D / Q182D / A216T / A246E, R89K / L90Y / E173T / S181T / N204G / T206D / A215D / A246N / S248C, V170L / S181D / Q182E / S212H / A246E, V170I / E173P / S181D / Q182E / A216T, R12L / V170L / T206D / A209E / A215D / A216T / D249L, T157D / E173P / A174G / Q182E / T206D / A215D / A216T, T176S / V177W / A246N / S248C, R89K / L90A / E173W / A215D / A216T / A246E / S248C, L90Y / T157G / A174G / T176S / A216T / A246Q / S248C, R12S / F92G / V170L / T176S / A209E / A215D / A216T, R12T / S248C / D249L, R12L / V170L / V177T / D203K / S212H / S248C / D249L, R12T / V170L / T176S / V177T / A209E, R12T / L90Y / F92G / V170I / V177I / S212E / A246Q / S248C, R12E / R89K / L90A / V170I / V177I / T206D / A215D / A216T, A174G / D203G / A216T / S248C / D249L, T157S / E173A / S181D / S248R / D249L, L90Y / E173V / S181D / T206D / S212Q / A246Q, T157Q / V170I / T176S / T206D / A215D / A246N / S248C, F92G / T176S / A216T, V170L / T176S / V177I / S181T / Q182D / D203A / S212E, F92G / V177W / A215D / A216T, L90Y / E173A / A174G / S181T / Q182D / A216T, F92G / V170I / A174G / T176S / D203N / A215D / A216T / A246E, V170I / T176S / S181Q / Q182E / T206D / S212Q, V170I / T176S / V177T / S181D / D203K / S212E, L90Y / V170I / T176S / N204G / A216T, A174G / T176S / A246N / S248R, T157L / V170I / V177T / S181D / S212E, L90F / F92G / D203S, F92G / V170I / V177T, V170I / T176S / V177W, E173N / T176S / S181T / Q182D / A246E, and V170L / V177I / S181T / Q182D / T206D / A209E / A215D / A216T. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase that are more stable than as compared to SEQ ID NOs: 3, 5 or 10. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase are more stable than as compared to SEQ ID NO: 1. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr- PETase has improved acƟvity and / or thermostability at a pH above 6. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase has improved acƟvity and / or thermostability at a pH above 6 with respect to SEQ ID NO: 1. In another aspect, the present disclosure relates to a nucleic acid encoding the variant Bhr-PETase enzyme disclosed herein. In another aspect, the present disclosure relates to an expression vector comprising the nucleic acid. In another aspect, the present disclosure relates to a host cell comprising the expression vector. In some embodiments, the cell is bacteria, yeast or fungi. In another aspect, the present disclosure relates to a method of degrading PET, comprising contacƟng the PET with the variant Bhr-PETase enzyme described herein. In some embodiments, the method degrades the PET in a mixed plasƟcs composiƟon. In some embodiments, the plasƟcs composiƟon comprises analog of PET, PET-like or PET subsƟtute derived biologically or chemically. In some embodiments, the plasƟcs composiƟon comprises at least one selected from the group consisƟng of Polybutylene terephthalate (PBT), Polycabonate (PC), Polycaprolactone (PCL), Polyethylene Furanoate (PEF), and High Density Polyethylene (HDPE). In some embodiments, the contacƟng occurs at least 50°C. In some embodiments, the contacƟng occurs from about 50°C to about 75°C. In some embodiments, the contacƟng occurs from about 55°C to about 65°C. In some embodiments, the contacƟng occurs from about 55°C to about 60°C. In some embodiments, the contacƟng occurs at about 60°C BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 depicts the sequence of an exemplary wild-type Bhr-PETase (also referred to as G1P Bhr- PETase; SEQ ID NO:1). Figure 2 depicts the sequence alignment of Bhr-PETase G1P (SEQ ID NO:1), G2P (SEQ ID NO:3), G3P (SEQ ID NO:5), and G4P (SEQ ID NO:10). Figure 3 depicts the tabulated fold improvement in PET degradaƟon total acƟvity of G1P-derived variants (specifically G2P-derived variants) evaluated under high-throughput (HTP) condiƟons at 65- 68°C. Figures 4A–4B depict the tabulated fold improvement in protein Ɵter of G1P-derived variants (specifically G2P-derived variants) evaluated under scale-up shakeflask condiƟons. Figures 5A–5B depict the tabulated fold improvement in PET degradaƟon specific acƟvity of G1P- derived variants (specifically G2P-derived variants) evaluated evaluated under scale-up shakeflask condiƟons at 65-68°C. Figure 6 depicts the tabulated fold improvement in PET degradaƟon specific acƟvity of G1P-derived variants (specifically G2P-derived variants) evaluated evaluated under scale-up shakeflask condiƟons at 60°C. Figures 7A–7R depict the tabulated fold improvement in PET degradaƟon specific acƟvity of G1P- derived variants (specifically G4P-derived variants) evaluated under high-throughput (HTP) condiƟons at 65-68°C. Figure 8 depicts parƟcular G1P-derived variants of Bhr-PETase by amino acid posiƟon. As used herein, “G1P-derived” refers to variants may be variants that do not include the G3P C-terminal domain, including G1P, G2P, and G4P-derived variants. Figure 9 depicts parƟcular G3P-derived variants of Bhr-PETase by amino acid posiƟon. “G3P-derived” refers to variants that include an addiƟonal C-terminal domain to G2P variant. Figure 10 depicts the fold improvement in PET degradaƟon specific acƟvity over G4P as a funcƟon of mutaƟon rate (number of mutaƟons per variant relaƟve to G1P), based on results from high- throughput (HTP) screening. Figure 11 depicts the validaƟon performance of surrogate models trained to predict fold improvement in PET degradaƟon specific acƟvity over G4P. The models were trained using high- throughput (HTP) data and show Spearman’s ρ > 0.6 and Δρ < 0.1, indicaƟng strong predicƟve performance. Figure 12 depicts the comparison between predicted and experimentally measured fold improvement in PET degradaƟon specific acƟvity over G4P across the full dataset, based on high- throughput (HTP) format measurements. Figure 13 depicts the top 20 variants ranked by predicted fold improvement in PET degradaƟon specific acƟvity over G4P, using one surrogate model for demonstraƟon. Both predicted and experimentally measured values are shown; Both predicted and experimentally measured values are shown based on high-throughput (HTP) assay results; the majority of variants demonstrate validated improvement. Figure 14 depicts the fold improvement in PET degradaƟon specific acƟvity over G4P versus mutaƟon rate for variants generated by high-throughput (HTP) screening in Example 4 and AI-guided Bayesian opƟmizaƟon in Example 6. Figures 15A–15C depict a representaƟve set of 100 predicted variants with improved PET degradaƟon specific acƟvity over G4P, based on outputs from mulƟple predicƟve models. Reported values reflect the mean predicƟon across different model types. Figure 16 depicts the comparaƟve analysis of protein Ɵters of Bhr-PETase G1P, G2P, and G3P, evaluated under scale-up fermentor condiƟons. Figure 17 depicts the fold improvement in TPA % conversion achieved using the engineered G3P C- terminal domain compared to the wild-type HFB4 domain, when fused to Bhr-PETase via aflexible linker and tested under scale-up shakeflask condiƟons. Figures 18A–18M depict the tabulated fold improvement in protein Ɵter, PET degradaƟon total acƟvity, and PET degradaƟon specific acƟvity of G3P-derived variants evaluated under high- throughput (HTP) condiƟons at 65-68°C. Figure 19 depicts addiƟonal G3P-derived Bhr-PETase variants by amino acid posiƟon, including variants newly generated and those not previously shown in Figure 9. Thisfigure complements the performance data presented in Figures 18A–18M. Figures 20A–20B depict the tabulated fold improvement in PET degradaƟon specific acƟvity of nine G1P-derived variants (specifically G4P-derived variants), including seven AI-designed or raƟonally designed variants and two benchmark variants, evaluated under shakeflask condiƟons. Figure 20A shows measured fold improvement at a later Ɵme point (18–19 hours), while Figure 20B shows predicted and measured fold improvements at an early Ɵme point (6-hour predicƟon and 3-hour measurement). DETAILED DESCRIPTION OF THE INVENTION IntroducƟon. The present invenƟon is directed to enzymes that will hydrolyze polyethylene terephthalate (PET). PET is a polyester polymer created by the combinaƟon of two monomers: modified ethylene glycol and purified terephthalic acid. While plasƟcs such as PETfind literally thousands of uses in modern society, PET, is essenƟally non-degradable. As such, plasƟc polluƟon has contaminated the enƟre planet, which poses a number of significant issues for the planet and human health. PET can be recycled; however this sƟll does not prevent major amounts of PET from being dumped into landfill and / or the ocean. PlasƟcs including PET are remarkably resistant to enzymaƟc degradaƟon. There are two categories of PET hydrolases: (i) PET-modifying enzymes that limit the degradaƟon only at the surface of PET without visible change by electron microscope observaƟons and (ii) PET-degrading enzymes or PETases that can significantly degrade the inner block of PET (e.g., by at least 10%) with visible change by electron microscope observaƟons. To date, a plethora of PET-modifying enzymes have been reported but they may not significantly degrade the body of PET and may not be applicable for biorecycling of PET. As is known in the art, there are a few enzyme types that show limited ability to degrade the inner block of PET. Thefirst reported enzyme able to act on ester bonds of PET polymers was a cuƟnase from Thermobifida fusca in 2005. Subsequent work idenƟfied addiƟonal enzymes, including a PET-hydrolyzing enzyme from Ideonella sakaiensis, Is-PETase, in 2016 and a leaf branch compost cuƟnase (Lcc-PETase) in 2012. While these PETases show acƟvity at ambient temperatures, these enzymes are not parƟcularly thermostable and do not show robust PET degradaƟon. PET exists both as an amorphous and as a semi-crystalline material. Chain mobility may be increased in the amorphous phase around PET’s glass transiƟon temperature Tg (around 70°C), which allows beƩer access to ester linkages and hence faster degradaƟon. The reacƟon temperature around Tg may be controlled to achieve efficient enzymaƟc PET degradaƟon. In addiƟon, the physical aging process of PET at around 70°C may convert the mobile amorphous fracƟon to recalcitrant microstructures which hinders further enzymaƟc hydrolysis of PET. Therefore, a thermostable and thermoacƟve PETase is desirable to allow the degradaƟon reacƟon to occur at around glass transiƟon temperature and overcome the compeƟng physical aging process. The significance of this innovaƟon is exemplified in Figures 3, 4, 5, 6, 7, 18, 21 and 22, where novel G1P- and G3P-derived Bhr-PETase variants exhibit even greater protein Ɵter, PET degradaƟon total acƟvity and / or specific acƟvity. These pivotal metrics – protein Ɵter, PET degradaƟon total acƟvity and / or specific acƟvity – hold paramount importance in achieving efficient and cost-effecƟve PET degradaƟon. The present disclosure pertains to the development of PETase variants originaƟng from the bacterium HR29 (Bhr-PETase), which have undergone deliberate engineering to elevate their protein Ɵter, PET degradaƟon total acƟvity and / or specific acƟvity. The excepƟonal performance exhibited by these Bhr-PETase variants underscores their substanƟal potenƟal for further analysis and wide-ranging industrial applicaƟons. DefiniƟons By "modificaƟon" herein is meant an amino acid subsƟtuƟon, inserƟon, and / or deleƟon in a polypepƟde sequence or an alteraƟon to a moiety chemically linked to a protein. For example, a modificaƟon may be an altered carbohydrate or PEG structure aƩached to a protein. By "amino acid modificaƟon" herein is meant an amino acid subsƟtuƟon, inserƟon, and / or deleƟon in a polypepƟde sequence. For clarity, unless otherwise noted, the amino acid modificaƟon is always to an amino acid coded for by DNA, e.g. the 20 amino acids that have codons in DNA and RNA. By "amino acid subsƟtuƟon" or "subsƟtuƟon" herein is meant the replacement of an amino acid at a parƟcular posiƟon in a parent polypepƟde sequence with a different amino acid. In parƟcular, in some embodiments, the subsƟtuƟon is to an amino acid that is not naturally occurring at the parƟcular posiƟon, either not naturally occurring within the organism or in any organism. For example, the subsƟtuƟon P20T refers to a variant polypepƟde, in this case a PETase, in which the proline (P) at posiƟon 20 is replaced with threonine T. For clarity, a protein which has been engineered to change the nucleic acid coding sequence but not change the starƟng amino acid (for example exchanging CGG (encoding arginine) to CGA (sƟll encoding arginine) to increase host organism expression levels) is not an “amino acid subsƟtuƟon”; that is, despite the creaƟon of a new gene encoding the same protein, if the protein has the same amino acid at the parƟcular posiƟon that it started with, it is not an amino acid subsƟtuƟon. By "amino acid inserƟon" or "inserƟon" as used herein is meant the addiƟon of an amino acid sequence at a parƟcular posiƟon in a parent polypepƟde sequence. For example, -233E or 233E designates an inserƟon of glutamic acid aŌer posiƟon 233 and before posiƟon 234. AddiƟonally, - 233ADE or A233ADE designates an inserƟon of AlaAspGlu aŌer posiƟon 233 and before posiƟon 234. By "amino acid deleƟon" or "deleƟon" as used herein is meant the removal of an amino acid sequence at a parƟcular posiƟon in a parent polypepƟde sequence. For example, F250- or F250#, F250() or F250del designates a deleƟon of glutamic acid at posiƟon 250. AddiƟonally, FRS250- or FRS250# designates a deleƟon of the sequence PheArgSer that begins at posiƟon 250. By "parent polypepƟde" as used herein is meant a starƟng polypepƟde that is subsequently modified to generate a variant. The parent polypepƟde may be a naturally occurring polypepƟde, or a variant or engineered version of a naturally occurring polypepƟde. Parent polypepƟde may refer to the polypepƟde itself, composiƟons that comprise the parent polypepƟde, or the amino acid sequence that encodes it. In the present case, some embodiments uƟlize an exemplary wild-type Bhr-PETase (also called G1P Bhr-PETase; SEQ ID NO: 1; sequence shown in Figure 1) as the parent polypepƟde. By "variant protein" or "protein variant", or "variant" as used herein is meant a protein that differs from that of a parent protein by virtue of at least one amino acid modificaƟon. Protein variant may refer to the protein itself, a composiƟon comprising the protein, or the amino sequence that encodes it. The protein variant has at least one amino acid modificaƟon compared to the parent protein, e.g. from about one to about seventy amino acid modificaƟons, and from about one to aboutfive amino acid modificaƟons compared to the parent. As described below, in some embodiments the parent polypepƟde is a wild-type sequence, for example the exemplary wild-type Bhr-PETase designated “G1P” herein. As further discussed below, the protein variant sequence herein will possess at least about 80, 81, 82, 83, 84, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty with a parent protein sequence, at least about 90% idenƟty, at least about 95% idenƟty, at least 98% idenƟty, or at least 99% idenƟty. Variant protein can refer to the variant protein itself, composiƟons comprising the protein variant, or the DNA sequence that encodes it. Thus, by “variant PETase” herein is meant a novel PETase that has at least one amino acid modificaƟon in the amino acid sequence as compared to a parent PETase enzyme. Unless otherwise noted or as will be obvious from the context, the variant PETases of the invenƟon generally are compared to the wild-type G1P sequence. AddiƟonally, unless otherwise noted, the variant PETases of the invenƟon are enzymaƟcally acƟve, that is, there is detectable PETase acƟvity using the PETase assay described in Examples 4 and 5. As used herein, “G1P-derived Bhr-PETases” refer to Bhr-PETase variant proteins whose parent polypepƟde does not contain the engineered C-terminal PET-binding domain present in the G3P sequence. This includes variant PETases derived from G1P (SEQ ID NO:1), as well as from G2P (SEQ ID NO:3) and G4P (SEQ ID NO:10) parent polypepƟdes. All such variants share a common background that lacks the G3P C-terminal domain, and unless otherwise noted, retain enzymaƟc PETase acƟvity as defined herein. “G3P-derived Bhr-PETases” refer to variant PETases derived from a parent polypepƟde that includes the engineered G3P-specific C-terminal PET-binding domain, typically introduced via a linker fused to the C-terminus of the parent polypepƟde. G3P-derived variants may include addiƟonal amino acid subsƟtuƟons, but share this domain structure as a disƟnguishing feature. Unless otherwise noted, G3P-derived variants are enzymaƟcally acƟve and compared to the G3P parent sequence (SEQ ID NO:5). As used herein, "protein" herein is meant at least two covalently aƩached amino acids, which includes proteins, polypepƟdes, oligopepƟdes and pepƟdes. The pepƟdyl group generally comprise naturally occurring amino acids and pepƟde bonds. In addiƟon, polypepƟdes may include syntheƟc derivaƟzaƟon of one or more side chains or termini, glycosylaƟon, PEGylaƟon, circular permutaƟon, cyclizaƟon, linkers to other molecules, fusion to proteins or protein domains, and addiƟon of pepƟde tags or labels. By "residue" as used herein is meant a posiƟon in a protein and its associated amino acid idenƟty. For example, Proline 20 (also referred to as Pro20 or P20) is a residue at posiƟon 20 in the G1P parental enzyme. By "non-naturally occurring modificaƟon" as used herein is meant an amino acid modificaƟon that is not found in the parent (e.g. G1P) enzyme in nature. By "amino acid" and "amino acid idenƟty" as used herein is meant one of the 20 naturally occurring amino acids that are coded for by DNA and RNA. By "posiƟon" as used herein is meant a locaƟon in the sequence of a protein. In general, the posiƟon number (which is more fully discussed below) is relaƟve to thefirst amino acid of the mature PETase sequence, e.g. excluding the signal pepƟde. By “PETase” herein is meant a protein with PETase acƟvity. By “PETase acƟvity” herein is meant that in the absence of MHTase, the enzyme catalyzes the hydrolysis of PET to mono(hydroxyethyl)terephthalate (MHET) as the major product. In the presence of MHTase, which is the case of Example 4, MHTase will further convert MHET to terephthalic acid (TPA) and ethylene glycol (EG) as the major products of the enzymaƟc reacƟon. Enzymes having detectable PETase acƟvity in the assay outlined below and in Examples 4 and 5 are considered PETases herein. The PETase acƟvity may be measured as PET degradaƟon total acƟvity and / or PET degradaƟon specific acƟvity as described herein. By “idenƟty” in reference to two sequences herein is meant that the same amino acid is at the same posiƟon considering the alignment. The degree of idenƟty between an amino acid sequence of the present invenƟon ("invenƟon sequence") and the parent amino acid sequence referred to in the claims (e.g. for G1P, SEQ ID NO:1) is calculated as the number of exact matches in an alignment of the two sequences, divided by the length of the "invenƟon sequence," or the length of the SEQ ID NO:1, whichever is the shortest. The result is expressed in percent idenƟty as calculated below. For purposes of the present invenƟon, the mature polypepƟde disclosed in SEQ ID NO:1 is used to determine the corresponding amino acid residue in another PETase of the present invenƟon. The amino acid sequence of another PETase is aligned with the mature polypepƟde disclosed in SEQ ID NO:1, and based on the alignment, the amino acid posiƟon number corresponding to any amino acid residue in the mature polypepƟde disclosed in SEQ ID NO:1 is determined using the Needleman- Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol.48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open SoŌware Suite, Rice et al., 2000, Trends Genet.16: 276-277), preferably version 5.0.0 or later. The parameters used are gap open penalty of 10, gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) subsƟtuƟon matrix. IdenƟficaƟon of the corresponding amino acid residue in another PETase can be determined by an alignment of mulƟple polypepƟde sequences using several computer programs including, but not limited to, MUSCLE (mulƟple sequence comparison by log-expectaƟon; version 3.5 or later; Edgar, 2004, Nucleic Acids Research 32: 1792-1797), MAFFT (version 6.857 or later; Katoh and Kuma, 2002, Nucleic Acids Research 30: 3059-3066; Katoh et al. , 2005, Nucleic Acids Research 33: 511 -518; Katoh and Toh, 2007, BioinformaƟcs 23: 372-374; Katoh et al., 2009, Methods in Molecular Biology 537: 39-64; Katoh and Toh, 2010, BioinformaƟcs 26: 1899-1900), EMBOSS EMMA employing ClustalW (1.83 or later; Thompson et al., 1994, Nucleic Acids Research 22: 4673-4680), and EMBL-EBI employing Clustal Omega (Sievers and Higgins, 2014, Methods Mol Biol.2014;1079:105–16), using their respecƟve default parameters. When the other enzyme has diverged from the polypepƟde of SEQ ID NO:1 such that tradiƟonal sequence-based comparison fails to detect their relaƟonship (Lindahl and Elofsson, 2000, J. Mol. Biol. 295: 613-615), other pairwise sequence comparison algorithms can be used. Greater sensiƟvity in sequence-based searching can be aƩained using search programs that uƟlize probabilisƟc representaƟons of polypepƟde families (profiles) to search databases. For example, the PSI-BLAST program generates profiles through an iteraƟve database search process and is capable of detecƟng remote homologs (Atschul et al., 1997, Nucleic Acids Res.25: 3389-3402). Even greater sensiƟvity can be achieved if the family or superfamily for the polypepƟde has one or more representaƟves in the protein structure databases. Programs such as GenTHREADER (Jones, 1999, J. Mol. Biol.287: 797-815; McGuffin and Jones, 2003, BioinformaƟcs 19: 874-881) uƟlize informaƟon from a variety of sources (PSI-BLAST, secondary structure predicƟon, structural alignment profiles, and solvaƟon potenƟals) as input to a neural network that predicts the structural fold for a query sequence. Similarly, the method of Gough et al., 2000, J. Mol. Biol.313: 903-919, can be used to align a sequence of unknown structure with the superfamily models present in the SCOP database. These alignments can in turn be used to generate homology models for the polypepƟde, and such models can be assessed for accuracy using a variety of tools developed for that purpose. For proteins of known structure, several tools and resources are available for retrieving and generaƟng structural alignments. For example the SCOP superfamilies of proteins have been structurally aligned, and those alignments are accessible and downloadable. Two or more protein structures can be aligned using a variety of algorithms such as the distance alignment matrix (Holm and Sander, 1998, Proteins 33: 88-96) or combinatorial extension (Shindyalov and Bourne, 1998, Protein Engineering 11 : 739-747), and implementaƟon of these algorithms can addiƟonally be uƟlized to query structure databases with a structure of interest in order to discover possible structural homologs (e.g., Holm and Park, 2000, BioinformaƟcs 16: 566-567). In describing the variants of the present invenƟon, the nomenclature described below is adapted for ease of reference. The standardly accepted IUPAC single leƩer or three leƩer amino acid abbreviaƟon is employed. For an amino acid subsƟtuƟon, the following nomenclature is used herein: Original amino acid, posiƟon, subsƟtuted amino acid. Accordingly, the subsƟtuƟon of proline at posiƟon 20 with leucine is designated as “Pro27Thr” or “P20T”. MulƟple mutaƟons are separated by forward slash marks (“ / ”), e.g., “P20T / V23T”, represenƟng subsƟtuƟons at posiƟons 20 and 23, respecƟvely. AbbreviaƟon 1 leƩer Amino acid name AbbreviaƟon 1 leƩer Amino acid name proteins. In a parƟcular embodiment the PETase of the invenƟon is isolated. The term "isolated" as used herein refers to a polypepƟde which is at least 20% pure, at least 40% pure, at least 60% pure, at least 80% pure, at least 90% pure, at least 95% pure, at least 96% pure, at least 97% pure, at least 98% pure, or at least 99% pure as determined by SDS-PAGE. In parƟcular, the polypepƟdes are in "essenƟally pure form", i.e., that the polypepƟde preparaƟon is essenƟally free of other polypepƟde material with which it is naƟvely associated. This can be accomplished, for example, by preparing the polypepƟde by means of well-known recombinant methods or by classical purificaƟon methods. By “recombinant enzyme” herein is meant that the enzyme is produced by recombinant techniques and that nucleic acid encoding the enzyme of the invenƟon is operably linked to at least one exogeneous (e.g. not naƟve to the parent PETase) sequence, including, for examples, promoters, terminators, signal sequences, etc., as are more fully outlined below. The term “nucleic acid construct” refers to a nucleic acid molecule, either single-stranded or double- stranded, which is isolated from a naturally occurring gene or is modified to contain segments of nucleic acids in a manner that would not otherwise exist in nature or which is syntheƟc, and which comprises one or more control sequences. The term “operably linked” refers to a configuraƟon in which a control sequence is placed at an appropriate posiƟon relaƟve to the coding sequence of a polynucleoƟde such that the control sequence directs expression of the coding sequence. As used herein, the term “about” means modifying, for example, lengths of nucleoƟde sequences, degrees of errors, dimensions, the quanƟty of an ingredient in a composiƟon, concentraƟons, volumes, process temperature, process Ɵme, yields,flow rates, pressures, and like values, and ranges thereof, refers to variaƟon in the numerical quanƟty that may occur, for example, through typical measuring and handling procedures used for making compounds, composiƟons, concentrates or use formulaƟons; through inadvertent error in these procedures; through differences in the manufacture, source, or purity of starƟng materials or ingredients used to carry out the methods; and like consideraƟons. The term “about” also encompasses amounts that differ due to aging of, for example, a composiƟon, formulaƟon, or cell culture with a parƟcular iniƟal concentraƟon or mixture, and amounts that differ due to mixing or processing a composiƟon or formulaƟon with a parƟcular iniƟal concentraƟon or mixture. Whether modified by the term “about” the claims appended hereto include equivalents to these quanƟƟes. The term “about” further may refer to a range of values that are similar to the stated reference value. In certain embodiments, the term “about” refers to a range of values that fall within 10, 9, 8,7, 6, 5,4, 3, 2, 1 percent or less of the stated reference value. PETases of the InvenƟon Accordingly, the present invenƟon provides variant PETases with improved enzymaƟc acƟvity and protein Ɵter that can be used in a variety of applicaƟons, most notably in the degradaƟon of plasƟcs made from PET. In general, the variant PETases of the invenƟon have modified, improved biochemical properƟes as compared to the wild-type Bhr-PETase, “G1P” (i.e. “GeneraƟon 1 Parent”), SEQ ID NO:1 herein, “G2P” (i.e. “GeneraƟon 2 Parent”), which has amino acid subsƟtuƟon S27L, SEQ ID NO:3 herein, “G3P” (i.e. “GeneraƟon 3 Parent”), which has amino acid subsƟtuƟon S27L, linker and C-terminal domain, SEQ ID NO:5 herein, or “G4P” (i.e. “GeneraƟon 4 Parent”), which has amino acid subsƟtuƟons S27L / L90Y, SEQ ID NO:10 herein. Variant PETases derived from G1P, G2P, or G4P are referred to as “G1P-derived Bhr-PETases” herein. Variant PETases derived from G3P are referred to as “G3P-derived Bhr-PETases.” The biochemical properƟes of the variant PETases that can be improved herein include, but are not limited to, protein Ɵter, protein thermostability, PET degradaƟon total acƟvity, specific acƟvity, and thermoacƟvity. The variant Bhr-PETases of the invenƟon have one or more improved properƟes as compared to G1P, G2P, G3P, or G4P. By “improved” herein is meant a desirable change of at least one biochemical property. “Improved funcƟon” can be measured as a percentage increase or decrease of a parƟcular acƟvity, or as a “fold” change, with increases of desirable properƟes (e.g. acƟvity or thermostability). That is, a variant Bhr-PETase may have a 10% increase in thermostability or a 10% increase in PETase acƟvity, as compared to G1P, G2P, G3P, or G4P. In general, percentage changes are used to describe changes in biochemical acƟvity of less than 100%, and fold-changes are used to describe changes in biochemical acƟvity of greater than 100% (as compared to the parental enzyme, in many cases G1P, G2P, G3P, or G4P). In the present invenƟon, percentage changes (usually increases) of biochemical acƟvity of at least about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 98% and 99% can be accomplished. In the present invenƟon, a “fold increase” (or decrease) is measured as compared to the starƟng or parent enzyme. For example, as shown in the Figure 3, the variant S27L / L90Y / A174G / D203V / D249L has 1.32 fold increase in PET degradaƟon total acƟvity as compared to G2P: this is calculated by [(total acƟvity of variant) / (total acƟvity of parent)]. In many embodiments, the improvement is at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the improvement is 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In general, improvements are measured as compared to the G2P enzyme, G3P enzyme, or G4P enzyme using a Bhr-PETase acƟvity assay, under condiƟons that are relevant to industrial PET degradaƟon process. In some embodiments, beneficial subsƟtuƟons idenƟfied in G1P-derived Bhr- PETases also confer funcƟonal improvements when introduced into the G3P background. In general, improvements are measured as compared to the G1P enzyme using a Bhr-PETase acƟvity assay, under condiƟons that are relevant to industrial PET degradaƟon process. Total AcƟvity Increases The present invenƟon provides variant Bhr-PETases that have total acƟvity greater than the total acƟvity of G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C-terminal domain), or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y). The “total acƟvity” herein may be determined by monitoring the producƟon of TPA (terephthalic acid) during the PET depolymerizaƟon reacƟon at an elevated temperature such as 65°C, quanƟfied using a colorimetric assay or HPLC as described in Example 4. Any improvement in total acƟvity may be due to the improvement of thermoacƟvity, specific acƟvity, and / or protein Ɵter of the variant PETase. The present invenƟon provides variant Bhr-PETases that have total acƟvity greater than the total acƟvity of G1P (the wild-type Bhr-PETase of SEQ ID NO:1). In many embodiments, the variant Bhr-PETases have improved total acƟvity that is at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr-PETases have improved total acƟvity that is 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is at least 50°C, at least 51°C, at least 52°C, at least 53°C, at least 54°C, at least 55°C, at least 56°C, at least 57°C, at least 58°C, at least 59°C, at least 60°C, at least 61°C, at least 62°C, at least 63°C, at least 64°C, at least 65°C, at least 66°C, at least 67°C, at least 68°C, at least 69°C, or at least 70°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is 50°C, 51°C, 52°C, 53°C, 54°C, 55°C, 56°C, 57°C, 58°C, 59°C, 60°C, 61°C, 62°C, 63°C, 64°C, 65°C, 66°C, 67°C, 68°C, 69°C, 70°C or less. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 50°C to about 75°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 50°C to about 72°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 50°C to about 70°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 60°C to about 70°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 62°C to about 68°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 65°C to about 68°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 65°C to about 72°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 65°C to about 68°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 50°C to about 55°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 55°C to about 65°C. In many embodiments, the temperature of PET depolymerizaƟon reacƟon is from about 55°C to about 60°C. In general, improvements are measured as compared to the G2P, G3P, or G4P enzyme using a Bhr- PETase acƟvity assay, under condiƟons that are relevant to industrial PET degradaƟon process. In general, improvements are measured as compared to the G1P enzyme using a Bhr-PETase acƟvity assay, under condiƟons that are relevant to industrial PET degradaƟon process. (i) ThermoacƟvity Increases In one aspect, the variant Bhr-PETases may have increased thermoacƟvity. The “thermoacƟvity” herein may be determined by monitoring the producƟon of TPA (terephthalic acid), MHET (mono(hydroxyethyl)terephthalate) and BHET (bis(2-hydroxyl) terephthalate during the PET depolymerizaƟon reacƟon at an elevated temperature such as 65°C, quanƟfied in mg of equivalent TPA generated per h per mg of enzyme (mgTPAeq.h-1mgenzyme-1). Herein “Equivalent TPA” is calculated by a sum of TPA, MHET converted to TPA, BHET converted to TPA, and any other measurable oligomers converted to TPA. Thus, PETases that have increased acƟvity per milligram of enzyme as compared to G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C-terminal domain),or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y) may show an improved thermoacƟvity. In some embodiments, PETases that have increased acƟvity per milligram of enzyme as compared to G1P (the wild-type Bhr-PETase of SEQ ID NO:1), may show an improved thermoacƟvity. In many embodiments, the variant Bhr-PETases have improved thermoacƟvity that is at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr- PETases have improved thermoacƟvity that is 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In general, improvements are measured as compared to the G2P, G3P, or G4P enzyme using a Bhr- PETase acƟvity assay, under thermal condiƟons that challenge the variant Bhr-PETase against the G2P, G3P, or G4P enzyme. In general, improvements are measured as compared to the G1P, G2P, G3P, or G4P enzyme using a Bhr-PETase acƟvity assay, under thermal condiƟons that challenge the variant Bhr-PETase against the G1P, G2P, G3P, or G4P enzyme. (ii) Specific AcƟvity Increases In another aspect, the variant Bhr-PETases may have increased specific acƟvity. The “specific acƟvity” herein may be determined by monitoring the producƟon of TPA (terephthalic acid), MHET (mono(hydroxyethyl)terephthalate) and BHET (bis(2-hydroxyl) terephthalate during the PET depolymerizaƟon reacƟon at the opƟmal operaƟng temperature of Bhr-PETases, quanƟfied in mg of equivalent TPA generated per h per mg of enzyme (mgTPAeq.h-1mgenzyme-1). Thus, PETases that have increased acƟvity per milligram of enzyme as compared to G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C-terminal domain), or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y) may show an improved specific acƟvity. Thus, PETases that have increased acƟvity per milligram of enzyme as compared to G1P (the wild-type Bhr- PETase of SEQ ID NO:1) may show an improved specific acƟvity. In many embodiments, the variant Bhr-PETases have improved specific acƟvity that is at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr- PETases have improved specific acƟvity that is 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In some embodiments, the variant Bhr-PETases have increased equivalent TPA generated per h per mg of enzyme that is at least 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In some embodiments, the variant Bhr-PETases have increased equivalent TPA generated per h per mg of enzyme that is 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In general, improvements are measured as compared to the G2P, G3P or G4P enzyme, under condiƟons that are relevant to industrial PET degradaƟon process. In general, improvements are measured as compared to the G1P, G2P, G3P or G4P enzyme, under condiƟons that are relevant to industrial PET degradaƟon process. (iii) ProducƟon Increases In one aspect, the variant Bhr-PETases may have increased producƟon. The “producƟon” herein may be determined by monitoring the protein Ɵter of Bhr-PETase in g / L. Thus, PETases that have increased quanƟty per liter of enzyme supernatant as compared to G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C-terminal domain), or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y) may show an improved producƟon. Thus, PETases that have increased quanƟty per liter of enzyme supernatant as compared to G1P (the wild-type Bhr- PETase of SEQ ID NO:1), G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C- terminal domain), or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y) may show an improved producƟon. In many embodiments, the variant Bhr-PETases have improved thermoacƟvity that is at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr- PETases have improved thermoacƟvity that is 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In general, improvements are measured as compared to the G2P, G3P, or G4P enzyme using a Bhr- PETase protein quanƟficaƟon assay. In general, improvements are measured as compared to the G1P, G2P, G3P, or G4P enzyme using a Bhr-PETase protein quanƟficaƟon assay. Thermostability Increases AddiƟonally, as will be appreciated by those in the art, it can be desirable to run PET degradaƟon at around the glass transiƟon temperature. Amorphous PET domains will increase the mobility at around the glass transiƟon temperature (around 67-72°C), making it more accessible for enzyme hydrolysis. At higher temperatures above the transiƟon temperature, the PET substrate will re- crystalize over Ɵme, which is not favorable for PET degradaƟon. Therefore, for example, about 50- 72°C may be considered an opƟmal temperature range for PET degradaƟon. Accordingly, in many embodiments, the variant Bhr-PETases have improved thermostability. “Thermostability” in this context means that the variant enzymes are more stable than G1P (the wild-type Bhr-PETase of SEQ ID NO:1), G2P (the variant Bhr-PETase of SEQ ID NO:3 with amino acid subsƟtuƟon S27L), G3P (the variant Bhr-PETase of SEQ ID NO:5 with amino acid subsƟtuƟon S27L, linker and C-terminal domain), or G4P (the variant Bhr-PETase of SEQ ID NO:10 with amino acid subsƟtuƟons S27L / L90Y) under the same thermal challenge condiƟons, that is, the acƟvity of the variant is higher than that of the G1P, G2P, G3P, or G4P enzyme under idenƟcal condiƟons (generally using an assay as outlined herein). In some embodiments, the variant Bhr-PETases are more stable than the G2P, G3P, or G4P enzyme when exposed to temperatures of about 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85°C or more, and / or about 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85°C or less for a period of Ɵme, for example ranging from about 0.5, 1, 2, 3, 4, 5, or 6 hour to about 5, 6, 7, 8, 9, 10 hours or longer, depending on the ulƟmate condiƟons for the use of the variant Bhr-PETase. In some embodiments, the variant Bhr-PETases are more stable than the G2P, G3P, or G4P enzyme when exposed to temperatures from about 65°C to about 85°C for at least about 0.5 hour, from about 65°C to about 72°C for at least about 1 hour, at least 65°C for at least about 1 hour, at least 70°C for at least about 1.5 hour. In some embodiments, the variant Bhr-PETases are more stable than the G1P, G2P, G3P, or G4P enzyme when exposed to temperatures of about 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85°C or more, and / or about 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85°C or less for a period of Ɵme, for example ranging from about 0.5, 1, 2, 3, 4, 5, or 6 hour to about 5, 6, 7, 8, 9, 10 hours or longer, depending on the ulƟmate condiƟons for the use of the variant Bhr-PETase. In some embodiments, the variant Bhr-PETases are more stable than the G1P, G2P, G3P, or G4P enzyme when exposed to temperatures from about 65°C to about 85°C for at least about 0.5 hour, from about 65°C to about 72°C for at least about 1 hour, at least 65°C for at least about 1 hour, at least 70°C for at least about 1.5 hour. In some embodiments, the variant Bhr-PETases are more stable than the G2P, G3P, or G4P enzyme when exposed to temperatures from about 65°C to about 85°C for at most about 0.5 hour, from about 65°C to about 72°C for at most about 1 hour, at least 65°C for at most about 1 hour, at least 70°C for at most about 1.5 hour. In some embodiments, the variant Bhr-PETases are more stable than the G1P, G2P, G3P, or G4P enzyme when exposed to temperatures from about 65°C to about 85°C for at most about 0.5 hour, from about 65°C to about 72°C for at most about 1 hour, at least 65°C for at most about 1 hour, at least 70°C for at most about 1.5 hour. In some embodiments, the variant Bhr-PETases are more stable than the G2P, G3P, or G4P enzyme when exposed to temperatures from about 50°C to about 75°C, from about 50°C to about 55°C, from about 60°C to about 70°C, from about 65°C to about 75°C, from about 62°C to about 68°C, from about 65°C to about 68°C, or from about 65°C to about 72°C. In some embodiments, the variant Bhr- PETases are more stable than the G1P, G2P, G3P, or G4P enzyme when exposed to temperatures from about 50°C to about 75°C, from about 50°C to about 55°C, from about 60°C to about 70°C, from about 65°C to about 75°C, from about 62°C to about 68°C, from about 65°C to about 68°C, or from about 65°C to about 72°C. Accordingly, in many embodiments, the variant Bhr-PETases are more thermostable than the G2P, G3P, or G4P enzyme by at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr-PETases are more thermostable than the G2P, G3P, or G4P enzyme by 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. Accordingly, in many embodiments, the variant Bhr-PETases are more thermostable than the G1P, G2P, G3P, or G4P enzyme by at least 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or higher. In many embodiments, the variant Bhr-PETases are more thermostable than the G1P, G2P, G3P, or G4P enzyme by 1.05 fold, 1.1 fold, 1.2 fold, 1.3 fold, 1.4 fold, 1.5 fold, 1.6 fold, 1.7 fold, 1.8 fold, 1.9 fold, 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold, or 10 fold or lower. In many embodiments, the variant Bhr-PETase have improved thermostability at a pH above 6 above, above 6.5, above 7, above 7.5, above 8, above 8.5, above 9, above 9.5, above 10, above 10.5, above 11, above 11.5, above 12, above 12.5, above 13, or above 13.5. In many embodiments, the variant Bhr-PETase have improved thermostability at a pH below 14, below 13.5, below 13, below 12.5, below 12, below 11.5, below 11, below 10.5, below 10, below 9.5, below 9, below 8.5, below 8, below 7.5, below 7, below 6.5, or below 6. In many embodiments, the variant Bhr-PETase have improved thermostability at a pH above 6 and below 14, above 6 and below 13, above 6 and below 12, above 6 and below 11, above 6 and below 10, above 6 and below 9, above 6 and below 8, above 6 and below 7, above 6.5 and below 14, above 6.5 and below 13, above 6.5 and below 12, above 6.5 and below 11, above 6.5 and below 10, above 6.5 and below 9, above 6.5 and below 8, above 6.5 and below 7, above 7 and below 14, above 7 and below 13, above 7 and below 12, above 7 and below 11, above 7 and below 10, above 7 and below 9, above 7 and below 8, above 7.5 and below 14, above 7.5 and below 13, above 7.5 and below 12, above 7.5 and below 11, above 7.5 and below 10, above 7.5 and below 9, above 7.5 and below 8, above 8 and below 14, above 8 and below 13, above 8 and below 12, above 8 and below 11, above 8 and below 10, above 8 and below 9, above 9 and below 14, above 9 and below 13, above 9 and below 12, above 9 and below 11, or above 9 and below 10. In general, improvements are measured as compared to the G2P, G3P, or G4P enzyme using a Bhr- PETase acƟvity assay, under condiƟons that measure the variant Bhr-PETase, the G2P enzyme, the G3P enzyme, or the G4P enzyme acƟvity with and without thermal treatment. In general, improvements are measured as compared to the G1P, G2P, G3P, or G4P enzyme using a Bhr-PETase acƟvity assay, under condiƟons that measure the G1P, G2P, G3P, or G4P enzyme acƟvity with and without thermal treatment. PETase Assays There are several PETase acƟvity assays that can be used to determine acƟvity, as generally outlined in Examples 4 and 5 for PETfilm-based assay. PETase acƟvity can also be monitored by BHET-based assay or pNPB (p-Nitrophenyl Butyrate) assay. In pNPB assay, surrogate substrate p- nitrophenylbutyrate may be hydrolyzed by PETase to p-nitrophenol and butyric acid. The release of p-nitrophenol directly correlates to PETase acƟvity and can be determined spectrophotometrically at 405 nm, for example. G1P-Derived Bhr-PETases The present invenƟon provides a number of specific variant Bhr-PETases with improved acƟvity and / or thermostability for use in the degradaƟon of PET. In some embodiments, the variant Bhr-PETase has one or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G1P, SEQ ID NO:1) selected from the group consisƟng of 90, 27, 8, 12, 16, 20, 23, 24, 69, 89, 92, 109, 110, 112, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 212, 213, 215, 216, 218, 222, 223, 229, 246, 248 and 249. In some embodiments, the variant Bhr-PETase has 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G1P, SEQ ID NO:1) selected from the same group. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90Y, S27L, P8L, R12E, R12T, T16A, P20T, V23T, A24V, W69C, W69F, W69G, W69H, W69I, W69L, W69S, R89K, R89M, L90A, F92G, T109L, S110R, P112Q, T157A, T157D, T157G, T157L, T157Q, V170I, V170L, E173N, E173P, E173T, E173V, E173W, A174G, A174K, A174R, T176S, V177I, V177T, S181D, S181T, Q182E, H183A, H183D, H183S, H183T, H183Y, F187I, F187L, F187V, D203A, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, T206D, F208D, F208G, F208I, F208K, F208Q, F208S, F208T, F208V, S212N, S212Q, P213D, A215D, A216D, A216E, A216T, S218G, S218R, I222L, S223N, V229I, A246Q, S248R and D249L. In some embodiments, the variant Bhr-PETase has 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or more amino acid subsƟtuƟons selected from the same group. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the leucine at posiƟon 90 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is L90A. In some embodiments, the amino acid subsƟtuƟon is L90Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 27 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S27L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 8 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P8L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the arginine at posiƟon 12 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is R12E. In some embodiments, the amino acid subsƟtuƟon is R12T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 16 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T16A. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the proline at posiƟon 20 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P20T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 23 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V23T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 24 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A24V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the tryptophan at posiƟon 69 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine, with some embodiments not uƟlizing proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is W69C. In some embodiments, the amino acid subsƟtuƟon is W69F. In some embodiments, the amino acid subsƟtuƟon is W69G. In some embodiments, the amino acid subsƟtuƟon is W69H. In some embodiments, the amino acid subsƟtuƟon is W69I. In some embodiments, the amino acid subsƟtuƟon is W69L. In some embodiments, the amino acid subsƟtuƟon is W69S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the arginine at posiƟon 89 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is R89K. In some embodiments, the amino acid subsƟtuƟon is R89M. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 92 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F92G. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 109 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T109L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 110 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S110R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 112 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P112Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 157 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T157A. In some embodiments, the amino acid subsƟtuƟon is T157D. In some embodiments, the amino acid subsƟtuƟon is T157G. In some embodiments, the amino acid subsƟtuƟon is T157L. In some embodiments, the amino acid subsƟtuƟon is T157Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 170 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V170I. In some embodiments, the amino acid subsƟtuƟon is V170L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 173 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is E173N. In some embodiments, the amino acid subsƟtuƟon is E173P. In some embodiments, the amino acid subsƟtuƟon is E173T. In some embodiments, the amino acid subsƟtuƟon is E173V. In some embodiments, the amino acid subsƟtuƟon is E173W. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 174 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A174G. In some embodiments, the amino acid subsƟtuƟon is A174K. In some embodiments, the amino acid subsƟtuƟon is A174R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 176 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T176S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 177 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V177I. In some embodiments, the amino acid subsƟtuƟon is V177T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 181 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S181D. In some embodiments, the amino acid subsƟtuƟon is S181T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the glutamine at posiƟon 182 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is Q182E. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the hisƟdine at posiƟon 183 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is H183A. In some embodiments, the amino acid subsƟtuƟon is H183D. In some embodiments, the amino acid subsƟtuƟon is H183S. In some embodiments, the amino acid subsƟtuƟon is H183T. In some embodiments, the amino acid subsƟtuƟon is H183Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 187 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F187I. In some embodiments, the amino acid subsƟtuƟon is F187L. In some embodiments, the amino acid subsƟtuƟon is F187V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 203 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is D203A. In some embodiments, the amino acid subsƟtuƟon is D203G. In some embodiments, the amino acid subsƟtuƟon is D203K. In some embodiments, the amino acid subsƟtuƟon is D203L. In some embodiments, the amino acid subsƟtuƟon is D203N. In some embodiments, the amino acid subsƟtuƟon is D203R. In some embodiments, the amino acid subsƟtuƟon is D203S. In some embodiments, the amino acid subsƟtuƟon is D203V. In some embodiments, the amino acid subsƟtuƟon is D203Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 204 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N204G. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 206 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T206D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 208 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F208D. In some embodiments, the amino acid subsƟtuƟon is F208G. In some embodiments, the amino acid subsƟtuƟon is F208I. In some embodiments, the amino acid subsƟtuƟon is F208K. In some embodiments, the amino acid subsƟtuƟon is F208Q. In some embodiments, the amino acid subsƟtuƟon is F208S. In some embodiments, the amino acid subsƟtuƟon is F208T. In some embodiments, the amino acid subsƟtuƟon is F208V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 212 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S212N. In some embodiments, the amino acid subsƟtuƟon is S212Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 213 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P213D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 215 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A215D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 216 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A216D. In some embodiments, the amino acid subsƟtuƟon is A216E. In some embodiments, the amino acid subsƟtuƟon is A216T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 218 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S218G. In some embodiments, the amino acid subsƟtuƟon is S218R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the isoleucine at posiƟon 222 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is I222L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 223 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S223N. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 229 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V229I. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 246 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A246Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 248 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S248R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 249 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is D249L. In some embodiments, the variant Bhr-PETase enzyme has one or more amino acid subsƟtuƟons at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 of the posiƟons of SEQ ID NO: 1 as described above. In some embodiments, variant Bhr-PETases are encoded by an amino acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:1. In some embodiments, variant Bhr-PETases are encoded by an amino acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:3. In some embodiments, variant Bhr-PETases are encoded by an amino acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:10. G3P-Derived Bhr-PETases The present invenƟon provides a number of specific variant Bhr-PETases with improved acƟvity and / or thermostability for use in the degradaƟon of PET. In some embodiments, the variant Bhr-PETase has one or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G3P, SEQ ID NO:5) selected from the group consisƟng of 90, 12, 20, 23, 24, 69, 109, 110, 157, 174, 176, 177, 181, 183, 187, 203, 204, 208, 212, 213, 215, 216, 222, 229, and 246. In some embodiments, the variant Bhr-PETase has 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G3P, SEQ ID NO:5) selected from the same group. In some embodiments, the variant Bhr-PETase has one or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G3P, SEQ ID NO:5) selected from the group consisƟng of 90, 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248, and 249. In some embodiments, the variant Bhr-PETase has one or more amino acid subsƟtuƟons at a posiƟon (relaƟve to G3P, SEQ ID NO:5) selected from the group consisƟng of 90, 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248, and 249. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90A, L90F, L90Y, R12E, R12F, R12L, R12M, R12Q, R12S, R12T, A14V, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, T109L, S110R, H156N, T157A, T157D, T157E, T157G, T157L, T157Q, T157S, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, A174G, A174R, T176S, V177I, V177T, V177W, S181A, S181D, S181Q, S181T, Q182D, Q182E, Q182S, H183S, H183T, H183Y, F187I, F187L, D203A, D203E, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, N204S, T206D, T206S, F208D, F208G, F208Q, F208S, F208T, F208V, A209E, N211Q, S212E, S212H, S212I, S212Q, P213A, P213D, N214D, A215D, A215K, A215S, A216D, A216E, A216I, A216T, I217M, I222L, V229I, A246E, A246N, A246Q, S248C, S248R and D249L. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, S110R, T157A, T157L, A174R, T176S, V177T, S181D, H183S, H183T, H183Y, F187I, F187L, D203A, D203K, D203R, D203S, N204G, F208D, F208G, F208Q, F208S, F208T, F208V, S212Q, P213D, A215D, A216D, A216E, A216T, I222L, V229I, and A246Q. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, T157D, T157E, T157G, T157Q, T157S, A174G, V177I, V177W, S181A,S181Q, S181T, D203E, D203G, D203L, D203N, D203V, D203Y, N204S, S212E, S212H, S212I, P213A, A215K, A215S, A216I, A246E, and A246N. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, V177I, V177W, S181A, S181Q, S181T, N204S, S212E, S212H, S212I, P213A, A215K, and A215S. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of L90A, L90F, T157D, T157E, T157G, T157Q, T157S, A174G, D203E, D203G, D203L, D203N, D203V, D203YA216I, A246E, and A246N. In some embodiments, the variant Bhr-PETase has one more amino acid subsƟtuƟons selected from the group consisƟng of A14V, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, T109L, H156N, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, Q182D, Q182E, Q182S, T206D, T206S, A209E, N211Q, N214D, I217M, S248C, S248R and D249L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the leucine at posiƟon 90 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is L90A. In some embodiments, the amino acid subsƟtuƟon is L90F. In some embodiments, the amino acid subsƟtuƟon is L90Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the arginine at posiƟon 12 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is R12E. In some embodiments, the amino acid subsƟtuƟon is R12F. In some embodiments, the amino acid subsƟtuƟon is R12L. In some embodiments, the amino acid subsƟtuƟon is R12M. In some embodiments, the amino acid subsƟtuƟon is R12Q. In some embodiments, the amino acid subsƟtuƟon is R12S. In some embodiments, the amino acid subsƟtuƟon is R12T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 14 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A14V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the proline at posiƟon 20 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P20T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 23 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V23T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 24 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A24V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the tryptophan at posiƟon 69 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is W69F. In some embodiments, the amino acid subsƟtuƟon is W69H. In some embodiments, the amino acid subsƟtuƟon is W69I. In some embodiments, the amino acid subsƟtuƟon is W69L. In some embodiments, the amino acid subsƟtuƟon is W69S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparagine at posiƟon 87 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N87K. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the arginine at posiƟon 89 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is R89I. In some embodiments, the amino acid subsƟtuƟon is R89K. In some embodiments, the amino acid subsƟtuƟon is R89M. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 91 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is D91N. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 92 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F92D. In some embodiments, the amino acid subsƟtuƟon is F92G. In some embodiments, the amino acid subsƟtuƟon is F92Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 109 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T109L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 110 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S110R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the hisƟdine at posiƟon 156 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is H156N. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 157 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T157A. In some embodiments, the amino acid subsƟtuƟon is T157D. In some embodiments, the amino acid subsƟtuƟon is T157E. In some embodiments, the amino acid subsƟtuƟon is T157G. In some embodiments, the amino acid subsƟtuƟon is T157L. In some embodiments, the amino acid subsƟtuƟon is T157Q. In some embodiments, the amino acid subsƟtuƟon is T157S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 170 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V170I. In some embodiments, the amino acid subsƟtuƟon is V170L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the glutamic acid at posiƟon 173 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is E173A. In some embodiments, the amino acid subsƟtuƟon is E173N. In some embodiments, the amino acid subsƟtuƟon is E173P. In some embodiments, the amino acid subsƟtuƟon is E173R. In some embodiments, the amino acid subsƟtuƟon is E173T. In some embodiments, the amino acid subsƟtuƟon is E173V. In some embodiments, the amino acid subsƟtuƟon is E173W. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 174 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A174G. In some embodiments, the amino acid subsƟtuƟon is A174R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 176 of SEQ ID NO:1. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T176S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 177 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V177I. In some embodiments, the amino acid subsƟtuƟon is V177T. In some embodiments, the amino acid subsƟtuƟon is V177W. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 181 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S181A. In some embodiments, the amino acid subsƟtuƟon is S181D. In some embodiments, the amino acid subsƟtuƟon is S181Q. In some embodiments, the amino acid subsƟtuƟon is S181T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the glutamine at posiƟon 182 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is Q182D. In some embodiments, the amino acid subsƟtuƟon is Q182E. In some embodiments, the amino acid subsƟtuƟon is Q182S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the hisƟdine at posiƟon 183 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is H183S. In some embodiments, the amino acid subsƟtuƟon is H183T. In some embodiments, the amino acid subsƟtuƟon is H183Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 187 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F187I. In some embodiments, the amino acid subsƟtuƟon is F187L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 203 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is D203A. In some embodiments, the amino acid subsƟtuƟon is D203E. In some embodiments, the amino acid subsƟtuƟon is D203G. In some embodiments, the amino acid subsƟtuƟon is D203K. In some embodiments, the amino acid subsƟtuƟon is D203L. In some embodiments, the amino acid subsƟtuƟon is D203N. In some embodiments, the amino acid subsƟtuƟon is D203R. In some embodiments, the amino acid subsƟtuƟon is D203S. In some embodiments, the amino acid subsƟtuƟon is D203V. In some embodiments, the amino acid subsƟtuƟon is D203Y. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparagine at posiƟon 204 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N204G. In some embodiments, the amino acid subsƟtuƟon is N204S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the threonine at posiƟon 206 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T206D. In some embodiments, the amino acid subsƟtuƟon is T206S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the phenylalanine at posiƟon 208 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is F208D. In some embodiments, the amino acid subsƟtuƟon is F208G. In some embodiments, the amino acid subsƟtuƟon is F208Q. In some embodiments, the amino acid subsƟtuƟon is F208S. In some embodiments, the amino acid subsƟtuƟon is F208T. In some embodiments, the amino acid subsƟtuƟon is F208V. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 209 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A209E. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparagine at posiƟon 211 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N211Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 212 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S212E. In some embodiments, the amino acid subsƟtuƟon is S212H. In some embodiments, the amino acid subsƟtuƟon is S212I. In some embodiments, the amino acid subsƟtuƟon is S212Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the proline at posiƟon 213 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P213A. In some embodiments, the amino acid subsƟtuƟon is P213D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparagine at posiƟon 214 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N214D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 215 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A215D. In some embodiments, the amino acid subsƟtuƟon is A215K. In some embodiments, the amino acid subsƟtuƟon is A215S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 216 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A216D. In some embodiments, the amino acid subsƟtuƟon is A216E. In some embodiments, the amino acid subsƟtuƟon is A216I. In some embodiments, the amino acid subsƟtuƟon is A216T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the isoleucine at posiƟon 217 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is I217M. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the isoleucine at posiƟon 222 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is I222L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the valine at posiƟon 229 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V229I. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the alanine at posiƟon 246 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A246E. In some embodiments, the amino acid subsƟtuƟon is A246N. In some embodiments, the amino acid subsƟtuƟon is A246Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the serine at posiƟon 248 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S248C. In some embodiments, the amino acid subsƟtuƟon is S248R. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 249 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is D249L. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 12 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 12 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is R12T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 176 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 176 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is T176S. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 177 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 177 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and tryptophan, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is V177T. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 181 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 181 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S181D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 204 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 204 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is N204G. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 212 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 212 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is S212Q. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 213 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 213 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely alanine, arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon). In some embodiments, the amino acid subsƟtuƟon is P213D. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 215 of SEQ ID NO:1. In some embodiments, the variant Bhr-PETase has an amino acid subsƟtuƟon of the asparƟc acid at posiƟon 215 of SEQ ID NO:5. In some embodiments, the subsƟtuƟon is with any other of the 19 naturally occurring amino acids, namely arginine, asparagine, asparƟc acid, cysteine, glutamic acid, glutamine, glycine, hisƟdine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, tryptophan and valine, with some embodiments not uƟlizing cysteine (due to possible disulfide formaƟon) or proline (due to steric effects). In some embodiments, the amino acid subsƟtuƟon is A215D. In some embodiments, the variant Bhr-PETase enzyme has one or more amino acid subsƟtuƟons at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 of the posiƟons of SEQ ID NO: 5 as described above. In some embodiments, variant Bhr-PETases are encoded by an amino acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:5. In another aspect, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:5. In some embodiments, the present disclosure relates to a composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:5 wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity. In some embodiments, the composiƟon comprises a variant Bhr-PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249. In some embodiments, the variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249. In some embodiments, the variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249. In some embodiments, the amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, T157D, T157E, T157G, T157Q, T157S, A174G, D203E, D203G, D203L, D203N, D203V, D203Y, A216I, A246E and A246N. In some embodiments, the amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, V177I, V177W, S181A, S181Q, S181T, N204S, S212E, S212H, S212I, P213A, A215K, and A215S. In some embodiments, the amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, T157D, T157E, T157G, T157Q, T157S, A174G, V177I, V177W, S181A, S181Q, S181T, D203E, D203G, D203L, D203N, D203V, D203Y, N204S, S212E, S212H, S212I, P213A, A215K, A215S, A216I, A246E and A246N. In some embodiments, the amino acid subsƟtuƟon is selected from the group consisƟng of A14V, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, H156N, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, Q182D, Q182E, Q182S, T206D, T206S, A209E, N211Q, N214D, I217M, S248C, S248R and D249L. In some embodiments, the amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, L90Y, R12E, R12F, R12L, R12M, R12Q, R12S, R12T, A14V, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, T109L, S110R, H156N, T157A, T157D, T157E, T157G, T157L, T157Q, T157S, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, A174G, A174R, T176S, V177I, V177T, V177W, S181A, S181D, S181Q, S181T, Q182D, Q182E, Q182S, H183S, H183T, H183Y, F187I, F187L, D203A, D203E, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, N204S, T206D, T206S, F208D, F208G, F208Q, F208S, F208T, F208V, A209E, N211Q, S212E, S212H, S212I, S212Q, P213A, P213D, N214D, A215D, A215K, A215S, A216D, A216E, A216I, A216T, I217M, I222L, V229I, A246E, A246N, A246Q, S248C, S248R and D249L. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of W69F, W69I, W69L, H183S, H183Y, D203R, F208Q and F208V. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of N204G, S212Q, P213D and A215D. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, A174R, T176S, V177T, S181D, H183S, H183T, H183Y, D203A, D203G, D203R, D203S, N204G, F208D, F208Q, F208V, S212Q, P213D, A215D, I222L and V229I. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of D203A, D203R, and D203S. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of S181D, R12T, V177T, L90Y / T176S, T176S, A215D, and N204G. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of D203A, S181D, D203R, R12T, V177T, L90Y / T176S, T176S, A215D, N204G, and D203S. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / A246E, A174G / D203A / A216E, L90Y / A174G, and D203G. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12F / L90Y, R12T / L90A / T176S / S212E, and T176S / V177T / A215D / A246E. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, R12Q / T176S, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, and T176S / D203A / A216I. In some embodiments, the variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q,R12F / L90Y, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / A246E, A174G / D203A / A216E, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, R12Q / T176S, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, L90Y / A174G, T176S / D203A / A216I, D203G, R12T / L90A / T176S / S212E, and T176S / V177T / A215D / A246E. In some embodiments, the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12E / V170I / V177T / S212H, R12F / V170L / T176S / V177I / S181D, R12F / T157L / V170I / T176S / D203A / S212E, L90F / V170I / T176S / V177I / Q182D / A216T, L90Y / T157Q / V170I / S181Q / Q182D, R12T / L90F / F92G / V170I / A215D, R12T / V170L / S181T / Q182D, L90Y / A174G / T176S / V177I / Q182E, L90Y / V170I / T176S / V177I / Q182D / A246E, R12E / R89K / L90F / V170I / T176S, R12M / V170I / T176S / V177I / S212Q, R12M / V170L / A174G / S181D, R12L / F92G / T157E / V170L / S181T / Q182D / T206D / S212Q / A246Q, V170I / S181Q / Q182E / D203Y / A215D, R12M / R89K / L90Y / V170L / T176S / V177I / T206D / S212H, R12L / R89K / L90Y / V170I / V177I / S181Q / Q182D, R12S / L90Y / A246N / S248R, R12S / Q182E / D203S / A216T, R12T / V170I / D203A / S212H, T157Q / V170L / T176S / V177T / A215D / A216T, R12E / E173A / V177T / A215D / A216T, F92G / T157E / A174G / T176S / V177W / S181D / A215D / A216T, E173T / S181D / D203N, R12S / V170L / A174G / S181D, E173P / S181T / D203R / A216T / A246E / S248C, R12T / A174G / S181T / Q182D / A246E / S248R, R12S / A215D / A216T / S248C, R12M / T157S / V170L / T176S / V177T / S181D / Q182D, R12T / Q182D / A215D / A216T, V170L / A174G / T176S / Q182D / S212Q / A246E, R12S / V170L / A215D / A216T / A246E, R12T / L90A / E173P / A174G / S181D / Q182E, R89M / T157G / S181T / Q182E, R89K / V170I / T176S / V177I / D203S, L90F / F92D / V170L / T176S / D203S / T206D / S212H, V170I / E173T / S181T / Q182D / D203G / A215D / A246E, V170L / T176S / Q182D, T157Q / V170I / V177T / T206D / S212Q / D249L, R12E / T157E / V170I / A174G / T176S / Q182D, R12F / T157Q / V170I / D203G / S212Q / D249L, R89K / L90F / V170I / V177T, R12T / E173A / A174G / S181T / Q182D, T157A / V170L / V177T, E173P / S181D, E173R / A174G / A209E / A215D / A246Q, L90F / V170I / Q182E / A215D, F92G / T157L / V170I / T176S / S181Q / Q182E / S212H, R12T / E173T / A174G / S181D / Q182D, R12T / R89K / L90Y / T157A / V170I / A174G / T176S, L90Y / V170I / V177I / A215D / A216T, V170I / V177I / T206D / A215D, L90F / T157E / V170I / T176S / V177I / S212H, R12T / V170I / T176S / V177T, V170I / T206D / S212Q, R12E / L90F / V170I / T176S / S181T / Q182D, V170I / T176S / V177T / A246E, V170I / T176S / D203K, L90F / F92G / V170I / S181Q / Q182D / T206D / A215D, T157A / D203V / A209E / A215D, T157E / V170L / V177I / Q182E / A215D / A246N, T157Q / V170I / S181D / S212H, V170I / T176S / V177I / Q182D / N204G / T206D, T157G / V170I / S181T / S212Q, L90Y / E173R / Q182D / N204G / T206D / S212H, R89K / L90F / T176S / V177I, T157S / E173T / A174G / S181T / Q182E / A215D / A246N, L90F / F92G / V170I / T176S / V177T, E173N / S181D / S212E, R12S / E173P / S181D, R12E / T157L / E173N / A174G / S181D / Q182D, R12T / V170I, R12T / T157Q / V170I / A174G / T176S / A215D / A216T, L90Y / E173N / S181T / Q182E, L90F / V170L / T176S / V177T / A215D, R12T / R89K / L90F / V170I / S181D / Q182D / D249L, R12T / L90A / E173N / A209E, V170I / A215D / A216T, V170L / V177T, V170L / V177T / T206D / A215D, V170I / E173T / S181D, L90A / T157S / V170L / V177I / T206D / S212Q, V170L / T176S / V177I / S181D / D249L, T157A / A174G / T176S / S181D / Q182D / A215D / A216T, L90F / F92G / T157Q / V170I / V177W / A209E / A215D / A216T, V170I / A174G / T176S / Q182E / T206D / A216T, L90Y / F92G / T157G / V170I / T176S / V177I / D203A / S212Q / A246E, T157G / A209E / A215D / A246Q, R89K / L90Y / T157S / A174G / S181T / Q182D, V170I / V177T, V170L / T176S / A215D, E173N / S181D / D203G / A209E / A215D / A216T, T157Q / V170I / V177T / A246E, R12T / L90F / E173R / S181Q / Q182E / A209E, F92G / T157Q / E173R / A174G / S181D / Q182D / A215D / A216T / S248R / D249L, R12T / R89K / L90Y / V170I, T157G / V170I / Q182E / D203V, F92G / T157Q / V170I, T206D, E173T / A174G / Q182D / D203G / A216T, L90Y / T157S / V170L, R12T / T157L / V170L / V177T / D203S / A215D, R12E / V170I / A174G / Q182D / D203R / A215D / A216T, R12S / R89K / T157L / V170I / T176S / S181D / Q182D / D203G / A216T, R12S / R89K / L90Y / T176S / V177I / Q182D / D203A, R12T / R89K / V170I / V177T, R12E / E173P / S181T / Q182D / A215D / A216T, R12S / L90Y / S181T / Q182D / D203R, R12E / L90Y / T157L / E173A / A215D / A216T / A246E, R12E / V170L / D203R / A215D / A246E, R12E / L90Y / V170I / E173T / Q182D / A215D / A246E, R12T / L90Y / V170L / A215D, R12E / V170I / V177T / D203R, R12E / V170L / T176S / A215D, R12E / F92G / V170I / A174G / T176S / D203R / A216T, R12E / V170I / E173P / S181T / D203R, R12E / L90Y / V170I / A174G / V177I / S181D / A215D / A216T / A246E / S248R, R12E / V170L / D203R / A246E, V170L / V177T / A246E, R12T / L90Y / V170I / D203G, R89K / L90Y / V177I / S181D / D203G / A215D / A216T, R12T / T157S / E173P / A174G / S181D / D203R / A246E / S248R, R12E / L90Y / V170L / A174G / S181T / Q182D, R12E / S181T / Q182D / D203R / A215D, R12E / L90F / F92G / E173A / S181D / D203R, R12S / V170L / T176S / S181D, R12S / V170I / V177T / D203R, R12T / L90Y / V170L / D203A, L90Y / V170I / T176S / V177T / S181D / D203R / A246E / S248R, R12S / V170I / T176S / V177T / S181D / Q182D / A246E, R12S / V170I / Q182D / A215D / A216T, R12T / L90Y / V170I / V177T, L90Y / E173P / A174G / S181D / D203G, R12S / R89K / L90A / V170I / A174G / T176S / S181D / Q182D, R12T / L90F / E173A / A174G / S181D / A216T / D249L, L90Y / V170I / T176S / V177I / S181D / Q182D / A246E / S248C, R12T / R89K / L90Y / T157A, L90Y / H156N / T157L / T176S / N204G / T206S / A215D, V170I / T176S / V177T / D203R / A246E / S248R, R12E / V170L / S181D / A215D, R12T / T157E / V170L / T176S / S181T / D203R / A215D / A246E, R12T / L90Y / V170L / V177I / A216T, R12E / L90Y / T157A / E173A / S181T / Q182D / A215D / A216T, L90Y / V170I / V177T / A216T, R12E / N87K / R89K / V170I / T176S / V177I / D203R / A215D / A216T / D249L, R12E / V170I / T176S / V177I / S181D / Q182D, L90Y / F92G / T157A / V170I / D203G / A216T / A246E / S248C, R12T / L90Y / V170L / T176S / V177T / D203R / A216T, R12T / L90F / V170I / S181D, R12T / L90Y / V170L / T176S / V177T / D203R / A246E, F92G / V170I / V177I / S181D, R12E / F92G / V170I / T176S / V177T / A215D / A216T / A246E, R12T / V170I / T176S / V177T / D203R, R12E / L90Y / E173P / A174G / S181T / Q182D, R12E / L90Y / V170I / T176S / S181T / D203R / A216T / A246E, R12T / D203R / D249L, R12E / L90Y / T157L / V170L / T176S / V177T / S181D / Q182D / D203R / A246E, R12S / V170L / T176S / V177I, L90Y / V170L / T176S / V177I, R12E / L90F / F92G / V170L / V177T / D203R / A216T, L90Y / V170L / V177T / D203G / A216T / A246E, V170I / A174G / S181D / D203R / A246E / S248R, R12E / T157E / T176S / Q182D / A215D / A216T, S181A / Q182S / D203S, R12S / V170I / S181D / D203R / A215D / A216T, R12E / V170L / T176S / S181D / Q182D / A216T, L90F / F92G / V170I / V177T / D203R / A216T, R12S / E173P / S181T / D203R / A215D / A216T / A246E, R12E / R89K / L90Y / D203R, R12E / F92G / A215D / A216T / A246E / S248R, R12S / T157S / E173T / A174G / D203R / A215D, R12E / L90A / E173P / A174G / S181T / Q182D / A215D / A216T, R89I / L90Y / H156N / T157L / V177I, L90Y / D249L, R89I / A174G / T176S / A215K, F92G / T157A / E173P / A174G / S181D / A216T, R12E / F92G / T176S / V177I, R12T / V170L / T176S / V177T / D203R / A216T, L90Y / F92Q / N204G / N211Q / A215S, L90Y / F92G / Q182S / N211Q / S212I / A216E / I217M, T176S / N211Q, L90F / D203R / D249L, R12T / L90Y / E173P / A174G / A246E, R89K / V170L / T176S / V177I / A215D, R89I / L90Y / T176S / S181A / Q182S / N204S / P213A / N214D, R12L / R89K / L90Y / V170L / T176S / V177I / N204G / A215D, R89K / V170L / T176S, T157S / V170I / V177T / S181T / Q182D / D203S / S212E / A246Q / S248C, D203G / A216E / I217M, R12T / R89K / T157G / D203N / S212E / A246E, E173R / A174G / Q182D / D203R / A215D / A216T / A246N / S248C, R12S / L90Y / V170I / E173A / A174G / S181T / Q182D / S248C / D249L, R12T / R89K / L90A / E173R / A174G / S181T / Q182D / A215D / A216T, R12T / V177T / D203V / A215D / A246N / S248C, R12T / V170L / V177T / S181T / A216T, R12T / R89K / L90Y / Q182D / A246E / S248C, R12E / F92G / V170L / V177T / A215D / S248C, R12T / L90A / D203Y / A209E / S212Q / A246Q, R12M / E173P / A174G / S181D / A209E / S212Q / A246E / S248R, R12T / R89K / L90Y / S212H / A215D / A246Q / S248C, R12T / R89K / L90A / A174G / T176S / S181T / Q182D / A215D / A246Q, R89K / L90F / A174G / S181D / S212E / A246E / S248C, R12M / E173R / S181T / Q182E / S212Q / A216T, R12L / R89K / L90Y / T157D / E173P / A174G / S181Q / Q182D / S212H, R12T / R89K / L90A / A215D / A216T / D249L, R12T / L90A / A246E / S248C, E173R / Q182D / A215D / A216T / S248R / D249L, R89K / L90Y / T176S / V177W / D203Y / A216T, R12M / V170I / A174G / T176S / S181T / T206D / A246Q / S248C, R12F / V170L / V177I / S181D / Q182E / S212E / A246E, R12T / R89K / L90F / V170L / T176S / S181Q / A216T, R12E / L90A / E173R / S181Q / Q182E / A209E / S212Q / A246N / S248C, R12T / T157Q / V170L / T176S / V177T / T206D / A209E / A216T, R12T / R89K, R12T / T157Q / V170I / E173N / S181T / Q182D / A215D / A216T / A246Q / S248C, R89K / L90Y / T206D / A209E / S212H / S248C, R12T / R89K / L90Y / A216T, R89K / T176S / V177I / S181Q / T206D / A209E / A215D / A216T / A246E, V170L / E173N / V177T / Q182D / T206D / A216T / A246Q, R12T / Q182E / D203V / A216T / A246E / S248C, L90Y / V170I / V177T / D203Y / S212E / A246E / S248C, R89K / L90Y / V170L / A174G / T176S / S181Q / S212E / A215D / A216T / A246E, T157Q / V170L / T176S / Q182D / A209E / A216T / A246Q, R12E / T157Q / E173R / A174G / Q182E / D203L / N204G / S212Q / A246E / S248C, R12M / A14V / V170I / V177T / S212H / A246E / S248C, R12L / L90A / D91N / F92G / A215D / A216T, R12T / V170L / V177T / A216T, L90A / S212Q / A246E / S248C, R12L / R89K / L90Y / V170I / V177I / D203R, R12T / S181D / Q182D / A216T / A246E, R89K / L90Y / E173T / S181T / N204G / T206D / A215D / A246N / S248C, V170L / S181D / Q182E / S212H / A246E, V170I / E173P / S181D / Q182E / A216T, R12L / V170L / T206D / A209E / A215D / A216T / D249L, T157D / E173P / A174G / Q182E / T206D / A215D / A216T, T176S / V177W / A246N / S248C, R89K / L90A / E173W / A215D / A216T / A246E / S248C, L90Y / T157G / A174G / T176S / A216T / A246Q / S248C, R12S / F92G / V170L / T176S / A209E / A215D / A216T, R12T / S248C / D249L, R12L / V170L / V177T / D203K / S212H / S248C / D249L, R12T / V170L / T176S / V177T / A209E, R12T / L90Y / F92G / V170I / V177I / S212E / A246Q / S248C, R12E / R89K / L90A / V170I / V177I / T206D / A215D / A216T, A174G / D203G / A216T / S248C / D249L, T157S / E173A / S181D / S248R / D249L, L90Y / E173V / S181D / T206D / S212Q / A246Q, T157Q / V170I / T176S / T206D / A215D / A246N / S248C, F92G / T176S / A216T, V170L / T176S / V177I / S181T / Q182D / D203A / S212E, F92G / V177W / A215D / A216T, L90Y / E173A / A174G / S181T / Q182D / A216T, F92G / V170I / A174G / T176S / D203N / A215D / A216T / A246E, V170I / T176S / S181Q / Q182E / T206D / S212Q, V170I / T176S / V177T / S181D / D203K / S212E, L90Y / V170I / T176S / N204G / A216T, A174G / T176S / A246N / S248R, T157L / V170I / V177T / S181D / S212E, L90F / F92G / D203S, F92G / V170I / V177T, V170I / T176S / V177W, E173N / T176S / S181T / Q182D / A246E, and V170L / V177I / S181T / Q182D / T206D / A209E / A215D / A216T. Transferability of Beneficial SubsƟtuƟons across Engineering Backgrounds While it is inherently challenging to idenƟfy idenƟcal beneficial variants across different library designs and experimental campaigns—parƟcularly given variaƟons in parent sequence, mutaƟon context, screening condiƟons, and the combinatorial explosion of possible subsƟtuƟon combinaƟons—a number of common variants have nonetheless been idenƟfied in both G1P-derived and G3P-derived backgrounds. Importantly, some of these overlapping variants were independently observed to confer improved properƟes in both backgrounds. For example, certain subsƟtuƟon combinaƟons led to increased specific acƟvity, protein Ɵter, or total acƟvity in both G1P-derived and G3P-derived backgrounds. These results suggest that at least a subset of beneficial subsƟtuƟons is transferable between engineered enzyme lineages. Accordingly, it is reasonable to postulate that subsƟtuƟons idenƟfied as beneficial on a G1P-derived background may also be beneficial when introduced into a G3P-derived background, and vice versa. The subsƟtuƟons proposed for transfer have already been validated in a well-characterized G1P-derived background, and their inclusion in the G3P-derived background is based on this experimentally supported design logic. The corresponding G3P-derived variants are included in the claims. Nucleic Acids of the InvenƟon The present invenƟon addiƟonal provides nucleic acids encoding the variant Bhr-PETases of the invenƟon. As will be appreciated by those in the art, due to the degeneracy of the geneƟc code, an extremely large number of nucleic acids may be made, all of which encode the variant Bhr-PETases of the present invenƟon. Thus, having idenƟfied a parƟcular amino acid sequence, those skilled in the art could make any number of different nucleic acids, by simply modifying the sequence of one or more codons in a way which does not change the amino acid sequence of the protein. Thus, providing the amino acid sequence allows the generaƟon of a very large number of different nucleic acid sequences encoding the proteins. In some embodiments, specific variant Bhr-PETases are encoded by specific nucleic acid sequences. In some embodiments, specific variant Bhr-PETases are encoded by a nucleic acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:2. In some embodiments, specific variant Bhr-PETases are encoded by a nucleic acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:4. In some embodiments, specific variant Bhr-PETases are encoded by a nucleic acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:6. In some embodiments, specific variant Bhr-PETases are encoded by a nucleic acid sequence having at least about 80, 81, 82, 83, 84, 85, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% idenƟty to SEQ ID NO:11. As is known in the art, the nucleic acids encoding the components of the invenƟon can be incorporated into expression vectors as is known in the art, and depending on the host cells used to produce the heterodimeric anƟbodies of the invenƟon. Generally the nucleic acids are operably linked to any number of regulatory elements (promoters, origin of replicaƟon, selectable markers, ribosomal binding sites, inducers, etc.). The expression vectors can be extra-chromosomal or integraƟng vectors. The nucleic acids and / or expression vectors of the invenƟon are then transformed into any number of different types of host cells as is well known in the art, including mammalian, bacterial, yeast, insect and / or fungal cells, with bacteria, yeast and fungifinding use in many embodiments. PreparaƟon of Variants The nucleic acids encoding the variant Bhr-PETases of the invenƟon can be prepared using any mutagenesis procedure known in the art, such as site-directed mutagenesis and syntheƟc gene construcƟon as are well known in the art. SyntheƟc gene construcƟon entails in vitro synthesis of a designed polynucleoƟde molecule to encode a polypepƟde of interest. Gene synthesis can be performed uƟlizing a number of techniques, such as the mulƟplex microchip-based technology described by Tian et al. (2004, Nature 432: 1050- 1054) and similar technologies wherein oligonucleoƟdes are synthesized and assembled upon photo- programmable microfluidic chips. A preferred technique is GenScript®. Regulatory sequences The present invenƟon also relates to nucleic acid constructs comprising a polynucleoƟde encoding a variant of the present invenƟon operably linked to one or more control sequences that direct the expression of the coding sequence in a suitable host cell under condiƟons compaƟble with the control sequences. The polynucleoƟde may be manipulated in a variety of ways to provide for expression of a variant. ManipulaƟon of the polynucleoƟde prior to its inserƟon into a vector may be desirable or necessary depending on the expression vector. The techniques for modifying polynucleoƟdes uƟlizing recombinant DNA methods are well known in the art. The control sequence may be a promoter, a polynucleoƟde which is recognized by a host cell for expression of the polynucleoƟde. The promoter contains transcripƟonal control sequences that mediate the expression of the variant. The promoter may be any polynucleoƟde that shows transcripƟonal acƟvity in the host cell including mutant, truncated, and hybrid promoters, and may be obtained from genes encoding extracellular or intracellular polypepƟdes either homologous or heterologous to the host cell. Promoters for bacteria, yeast and fungi are well known in the art. Exemplary operons for expression in lacƟc acid bacteria include S. thermophilus lactose operons or L. lacƟc lac ABCDFEGX operons, which have been successfully used to induce foreign gene expression in hosts (eg, Simons et al., 1993, J. Bact.175: 5186-5175; see Mollet et al., 1993, J. Bact.175: 4315-4324). Non-limiƟng addiƟonal examples of consƟtuƟve promoters for bacteria include lac promoter, trp promoter, tac promoter, T7 promoter, erm promoter, Ɵp promoter, nit promoter, and Sp6 promoter. Exemplary promoters for yeasts include, but not limited to, AOX1 promoter, ADH promoter, PH05 promoter, gal10 promoter, PKG promoter and GAP promoter. Other useful promoters for yeast host cells are described by Romanoset al., 1992, Yeast 8: 423-488. Moreover, examples of useful promoters for fungi vectors include, but not limit to, Aspergillus niger GLA promoter, Aspergillus nidulans GPD promoter, those derived from Aspergillus nidulans glycolyƟc genes, such as the adh3 promoter (McKnight et al., EMBO J.4:2093-2099,1985). Codon OpƟmizaƟon While the wild-type coding sequence of Bhr-PETase is shown in SEQ ID NO:1, those of skill in the art will recognize that for heterologous expression, codon opƟmizaƟon can be done to increase expression in any parƟcular host organism. Codon opƟmizaƟon can be employed with any of the variant Bhr-PETase polypepƟdes of the present invenƟon, in order to opƟmize expression in the host cell employed. Such methods are well known in the art and described in, for example, WO 2007 / 142954. In heterologous expression systems, opƟmizaƟon steps can improve the ability of the host to produce the desired variant Bhr-PETase polypepƟdes. Protein expression is governed by a host of factors including those that affect transcripƟon, mRNA processing, and stability and iniƟaƟon of translaƟon. The polynucleoƟde opƟmizaƟon steps can include steps to improve the ability of the host to produce the foreign protein as well as steps to assist the researcher in efficiently designing expression constructs. OpƟmizaƟon strategies can include, for example, the modificaƟon of translaƟon iniƟaƟon regions, alteraƟon of mRNA structural elements, and the use of different codon biases. The following paragraphs discuss potenƟal problems that may result in reduced heterologous protein expression, and techniques that may overcome these problems. In some embodiments, reduced heterologous protein expression results from a rare codon-induced translaƟonal pause. A rare codon-induced translaƟonal pause includes the presence of codons in the polynucleoƟde of interest that are rarely used in the host organism can have a negaƟve effect on protein translaƟon due to their scarcity in the available tRNA pool. One method of improving opƟmal translaƟon in the host organism includes performing includes performing codon opƟmizaƟon which can result in rare host codons being modified in the syntheƟc polynucleoƟde sequence. In some embodiments, reduced heterologous protein expression results from by alternate translaƟonal iniƟaƟon. Alternate translaƟonal iniƟaƟon can include a syntheƟc polynucleoƟde sequence inadvertently containing moƟfs capable of funcƟoning as a ribosome binding site (RBS). These sites can result in iniƟaƟng translaƟon of a truncated protein from a gene-internal site. One method of reducing the possibility of producing a truncated protein, which can be difficult to remove during purificaƟon, includes modifying putaƟve internal RBS sequences from an opƟmized polynucleoƟde sequence. In some embodiments, reduced heterologous protein expression occurs through repeat-induced polymerase slippage. Repeat-induced polymerase slippage involves nucleoƟde sequence repeats that have been shown to cause slippage or stuƩering of DNA polymerase which can result in frameshiŌ mutaƟons. Such repeats can also cause slippage of RNA polymerase. In an organism with a high G+C content bias, there can be a higher degree of repeats composed of G or C nucleoƟde repeats. Therefore, one method of reducing the possibility of inducing RNA polymerase slippage includes altering extended repeats of G or C nucleoƟdes. In some embodiments, reduced heterologous protein expression occurs through interfering secondary structures. Secondary structures can sequester the RBS sequence or iniƟaƟon codon and have been correlated to a reducƟon in protein expression. Stemloop structures can also be involved in transcripƟonal pausing and aƩenuaƟon. An opƟmized polynucleoƟde sequence can contain minimal secondary structures in the RBS and gene coding regions of the nucleoƟde sequence to allow for improved transcripƟon and translaƟon. In some embodiments, restricƟon sites can affect heterologous protein expression. By modifying restricƟon sites that could interfere with subsequent sub-cloning of transcripƟon units into host expression vectors a polynucleoƟde sequence can be opƟmized. OpƟmizing a DNA sequence can negaƟvely or posiƟvely affect gene expression or protein producƟon. For example, modifying a less-common codon with a more common codon may affect the half-life of the mRNA or alter its structure by introducing a secondary structure that interferes with translaƟon of the message. It may therefore be necessary, in certain instances, to alter the opƟmized message. All or a porƟon of a gene can be opƟmized. In some embodiments, the desired modulaƟon of expression is achieved by opƟmizing essenƟally the enƟre gene. In other embodiments, the desired modulaƟon will be achieved by opƟmizing part but not all of the gene. The codon usage of any coding sequence can be adjusted to achieve a desired property, for example high levels of expression in a specific cell type. The starƟng point for such an opƟmizaƟon may be a coding sequence with 100% common codons, or a coding sequence which contains a mixture of common and non-common codons. Two or more candidate sequences that differ in their codon usage can be generated and tested to determine if they possess the desired property. Candidate sequences can be evaluated by using a computer to search for the presence of regulatory elements, such as silencers or enhancers, and to search for the presence of regions of coding sequence which could be converted into such regulatory elements by an alteraƟon in codon usage. AddiƟonal criteria can include enrichment for parƟcular nucleoƟdes, e.g., A, C, G or U, codon bias for a parƟcular amino acid, or the presence or absence of parƟcular mRNA secondary or terƟary structure. Adjustment to the candidate sequence can be made based on a number of such criteria. Promising candidate sequences are constructed and then evaluated experimentally. MulƟple candidates may be evaluated independently of each other, or the process can be iteraƟve, either by using the most promising candidate as a new starƟng point, or by combining regions of two or more candidates to produce a novel hybrid. Further rounds of modificaƟon and evaluaƟon can be included. Modifying the codon usage of a candidate sequence can result in the creaƟon or destrucƟon of either a posiƟve or negaƟve element. In general, a posiƟve element refers to any element whose alteraƟon or removal from the candidate sequence could result in a decrease in expression of the therapeuƟc protein, or whose creaƟon could result in an increase in expression of a therapeuƟc protein. For example, a posiƟve element can include an enhancer, a promoter, a downstream promoter element, a DNA binding site for a posiƟve regulator (e.g., a transcripƟonal acƟvator), or a sequence responsible for imparƟng or modifying an mRNA secondary or terƟary structure. A negaƟve element refers to any element whose alteraƟon or removal from the candidate sequence could result in an increase in expression of the therapeuƟc protein, or whose creaƟon would result in a decrease in expression of the therapeuƟc protein. A negaƟve element includes a silencer, a DNA binding site for a negaƟve regulator (e.g., a transcripƟonal repressor), a transcripƟonal pause site, or a sequence that is responsible for imparƟng or modifying an mRNA secondary or terƟary structure. In general, a negaƟve element arises more frequently than a posiƟve element. Thus, any change in codon usage that results in an increase in protein expression is more likely to have arisen from the destrucƟon of a negaƟve element rather than the creaƟon of a posiƟve element. In addiƟon, alteraƟon of the candidate sequence is more likely to destroy a posiƟve element than create a posiƟve element. In some embodiments, a candidate sequence is chosen and modified so as to increase the producƟon of a therapeuƟc protein. The candidate sequence can be modified, e.g., by sequenƟally altering the codons or by randomly altering the codons in the candidate sequence. A modified candidate sequence is then evaluated by determining the level of expression of the resulƟng therapeuƟc protein or by evaluaƟng another parameter, e.g., a parameter correlated to the level of expression. A candidate sequence which produces an increased level of a therapeuƟc protein as compared to an unaltered candidate sequence is chosen. In some embodiments, one or a group of codons can be modified, e.g., without reference to protein or message structure and tested. AlternaƟvely, one or more codons can be chosen on a message- level property, e.g., locaƟon in a region of predetermined, e.g., high or low GC content, locaƟon in a region having a structure such as an enhancer or silencer, locaƟon in a region that can be modified to introduce a structure such as an enhancer or silencer, locaƟon in a region having, or predicted to have, secondary or terƟary structure, e.g., intra-chain pairing, inter-chain pairing, locaƟon in a region lacking, or predicted to lack, secondary or terƟary structure, e.g., intra-chain or inter-chain pairing. A parƟcular modified region is chosen if it produces the desired result. Methods which systemaƟcally generate candidate sequences are useful. For example, one or a group, e.g., a conƟguous block of codons, at various posiƟons of a syntheƟc nucleic acid sequence can be modified with common codons (or with non common codons, if for example, the starƟng sequence has been opƟmized) and the resulƟng sequence evaluated. Candidates can be generated by opƟmizing (or de-opƟmizing) a given “window” of codons in the sequence to generate afirst candidate, and then moving the window to a new posiƟon in the sequence, and opƟmizing (or de- opƟmizing) the codons in the new posiƟon under the window to provide a second candidate. Candidates can be evaluated by determining the level of expression they provide, or by evaluaƟng another parameter, e.g., a parameter correlated to the level of expression. Some parameters can be evaluated by inspecƟon or computaƟonally, e.g., the possession or lack thereof of high or low GC content; a sequence element such as an enhancer or silencer; secondary or terƟary structure, e.g., intra-chain or inter-chain paring. In some embodiments, the opƟmized nucleic acid sequence can express the variant Bhr-PETase polypepƟde of the invenƟon, at a level which is at least about 110%, 150%, 200%, 500%, 1,000%, 5,000% or even 10,000% of that expressed by nucleic acid sequence that has not been opƟmized. Staring with the amino acid sequence of a variant Bhr-PETase, a candidate DNA sequence can be designed. During the design of the syntheƟc DNA sequence, the frequency of codon usage can be compared to the codon usage of the host expression organism and rare host codons can be modified in the syntheƟc sequence. AddiƟonally, the syntheƟc candidate DNA sequence can be modified in order to remove undesirable enzyme restricƟon sites and add or alter any desired signal sequences, linkers or untranslated regions. The syntheƟc DNA sequence can be analyzed for the presence of secondary structure that may interfere with the translaƟon process, such as G / C repeats and stem- loop structures. Before the candidate DNA sequence is synthesized, the opƟmized sequence design can be checked to verify that the sequence correctly encodes the desired amino acid sequence. Finally, the candidate DNA sequence can be synthesized using DNA synthesis techniques, such as those known in the art. In some embodiments, the general codon usage in a host organism, such as any of those described herein, can be uƟlized to opƟmize the expression of the heterologous polynucleoƟde sequence in the host organism. The percentage and distribuƟon of codons that rarely would be considered as preferred for a parƟcular amino acid in the host expression system can be evaluated. Values of 5% and 10% usage can be used as cutoff values for the determinaƟon of rare codons. Host Cells and ProducƟon Strains In one aspect, the present disclosure relates to an expression vector comprising the nucleic acid encoding the variant Bhr-PETase described herein. In another aspect, the present disclosure also relates to a host cell comprising the expression vector. In some embodiments, the host cell is bacteria. In some embodiments, the host cell is yeast. In some embodiments, the host cell is fungi. In some embodiments, the host cell may be bacteria, including but not limited to E. coli, Bacillus. In some embodiments, the host cell may be yeast, including but not limited to Saccharomyces cerevisiae, Pichia. In some embodiments, the host cell may be fungi, including but not limited to A. niger, T. reseei, or Myceliophthora thermophila. The expression vector may be any of integraƟon vectors which are to be integrated into genome or autonomously replicaƟng plasmids in the selected host. In one embodiment, the vector can be stably maintained in the introduced cell, with the variant Bhr-PETase gene supported thereon in afit state for expression. The expression vector may be selected to be suitable for the specific host cells to which the vector is introduced. Specific examples available for use include but not limit to pBR322, pACYC184, pUC18, pKK223-2, pHSG398 (Takara Bio Inc.), pTrcHis (Invitrogen CorporaƟon) and pET11a (Stratagene CorporaƟon) in the case where Escherichia coli is used as a host; pBBR122 (Mobiotech) and pBHR1 (Mobiotech) for the other gram-negaƟve bacteria; pHW1520 (Mobiotech) and pHY300PLK (Takara Bio Inc.) for Bacillus; pSH19 (Herai et al., Proc. Natl. Acad. Sci., 101, 14031- 14035, 2004), pIJ702 (John Innes Centre), pIJ943 (John Innes Centre), pIJ8600 (John Innes Centre), pIJ602 (John Innes Centre), 1, pTip-vectors (Nakashima et al., Appli. Environ. Microbiol., 70, 5557- 5568, 2004), pTYM19 (Onaka et al., J. AnƟbiot., 56, 950-956, 2003) for acƟnomycetes; pPICZα and pPIC9 (Thermo Fisher ScienƟfic) for Pichia, and pAO815 (Invitrogen CorporaƟon), pAUR101 (Takara Bio Inc.), pAUR123 (Takara Bio Inc.) and pAUR316 (Takara Bio Inc.) for fungi. In another aspect, the present disclosure also relates to a method of expressing the variant Bhr- PETase in the host cell. In another aspect, the present disclosure also relates to a method of making the variant Bhr-PETase comprising culturing the host cell under condiƟons wherein said variant Bhr- PETase is produced, and recovering said variant Bhr-PETase. The culture of a transformed organism may be performed in the medium which can be a nutriƟve medium of the transformed host cell without affecƟng the transformaƟon of the variant Bhr-PETase. Such a medium comprises an appropriate carbon source, nitrogen source, inorganic salt, natural organic nutrient and the like. As a carbon source, glucose, fructose, glycerol, sorbitol, organic acids can be used individually or in combinaƟon. The concentraƟon of the carbon source is not parƟcularly limited and may be 1 to 10 %. As a nitrogen source, ammonium, urea, ammonium sulfate, ammonium nitrate, ammonium acetate and the like can be used individually or in combinaƟon of two or more members thereof. As an inorganic salt, salts such as monopotassium phosphate, dipotassium phosphate, magnesium sulfate, manganese sulfate and ferrous sulfate can be used. In addiƟon, as an organic nutrient source having growth-promoƟng effects of the bacteria to be used, peptone, meat extract, yeast extract, corn steep liquor and casamino acids can be used and furthermore, a small amount vitamins and nucleic acids may be contained in the medium. PETase FormulaƟons and Uses As will be appreciated by those in the art, the formulaƟon of the variant Bhr-PETases of the invenƟon depends on its end use and the associated condiƟons. Suitable formulaƟons for the variant Bhr- PETases of the invenƟon include liquid formulaƟons, dried formulaƟons (including spray dried formulaƟons), powdered formulaƟons, granular formulaƟons, and pelleted formulaƟons. Bhr- PETases can also be formulated as “embedded in PET parƟcles” for natural degradaƟon. In some embodiments, the enzyme composiƟon (i.e., polypepƟde composiƟons) of the present invenƟon can be in any form suitable for use, such as, for example, a crude fermentaƟon broth with or without cells removed, a cell lysate with or without cellular debris, a semi-purified or purified enzyme composiƟon, or a host cell, as a source of the enzymes. In some embodiments, the enzyme composiƟon may be a dry powder or granulate, a non-dusƟng granulate, a liquid, a stabilized liquid, or a stabilized protected enzyme. Liquid enzyme composiƟons may, for instance, be stabilized by adding stabilizers such as a sugar, a sugar alcohol or another polyol, and / or lacƟc acid or another organic acid according to established processes. In some embodiments, the dosage of the polypepƟde composiƟon of the invenƟon and other condiƟons under which the composiƟon is used may be determined on the basis of methods known in the art. The above composiƟons are suitable for use in PET biodegradaƟon, PET biocycling, PET upcycling, and / or PET surface modificaƟon processes. In some embodiments, the above composiƟons are used to degrade pretreated PET. PET pretreatment may be performed before the enzymaƟc degradaƟon step. Commonly used PET pretreatment can be broadly classified into a) mechanical pretreatment, b) thermo-mechanical pretreatment, and c) chemical pretreatment. The mechanical process may involve grinding of the PETfilm into parƟcles less than about 500 µm post sieving. This process could be combined with immersing the PETfilm into liquid nitrogen mainly for ease of the process of grinding. The advantage of this type of mechanical pretreatment may be enzyme accessibility due to reduced parƟcle size. In the thermo-mechanical degradaƟon, the main-chain scission reacƟons may affect the stability of the cyclic oligomers. In the thermo-mechanical pretreatment, highly crystalline PETflakes may be amorphized at very high temperatures (> 260°C) using extruder equipped with melt pump and later micronized to parƟcle size of less than 500µm. An example of a commercial method of pretreaƟng PET is by using an extrusion cast process, namely molten polymer cast onto chilled rolls of PET to solidify. The thermo-mechanical pretreatment may lower the crystallinity as well as parƟcle size of the industrial grade PET allowing accessibility of the enzymes to depolymerize it. In case of chemical pretreatment, ionic liquid, strong acid, base, solvents etc. can be used to reduce crystallinity or to change the surface structure of the PET to facilitate access of the enzymes to further depolymerize it to its monomers. The two significant stumbling blocks in recycling plasƟcs irrespecƟve of using chemical or biological method may be material variability and the costs associated with idenƟfying and separaƟng waste plasƟcs into recognizable grade ranges. Different types and grades of plasƟcs differ in densiƟes and molecular weights. The advantage of knowing the properƟes of a parƟcular plasƟc that is in the recycling process would make it easier to determine its value and durability when put into valuable second use applicaƟons. But, the process of sorƟng is a major hindrance from the standpoint of resource uƟlizaƟon, Ɵme management, and cost effecƟveness. Hence, when looking at biological miƟgaƟons to overcoming the roadblocks associated with differenƟal plasƟc sorƟng, depolymerizaƟon of mixed plasƟcs using robust enzymes that has broad substrate specificity becomes the utmost priority. Mixed plasƟc is a term that covers all non-boƩle plasƟc packaging sourced from the domesƟc waste stream, and it includes rigid andflexible plasƟc items of various polymer types and colours that are typically found in the household waste bin. The mixed plasƟc refers to a mixture of different plasƟcs. Various polymer types may refer to PET and / or analog of PET, PET-like or PET subsƟtute derived biologically or chemically. Examples of analog of PET, PET-like or PET subsƟtute include but are not limited to Polybutylene terephthalate (PBT), Polycabonate (PC), Polycaprolactone (PCL), Polyethylene Furanoate (PEF) and High Density Polyethylene (HDPE). Bhr- PETase alone or in conjuncƟon with other accessory enzyme (s) can revoluƟonize the biological method of depolymerizaƟon of mixed plasƟcs. The process of enzymaƟc depolymerizaƟon of mixed plasƟcs as opposed to chemical methodologies may be environmentally safer and capable of retaining the market value of the second use applicaƟons. Such biological treatments can also be combined with mild chemical or thermomechanical pre-treatment to achieve higher depolymerizaƟon efficiencies. AŌer PET pretreatment and enzymaƟc degradaƟon, products may be recycled and generate other valuable chemicals. In one embodiment, TPA (terephthalic acid) is purified using an industry relevant process, and the process also may produce sodium sulphate, a chemical commonly used in the detergent, paper, and glass industries. In another embodiment, recycled TPA is used as the starƟng material to synthesize virgin PET. PET synthesized from recycled TPA may demonstrate similar properƟes, such as average molecular weight and intrinsic viscosity, as PET synthesized using petrochemical TPA. In one embodiment, boƩles blown from recycled PET exhibits similar mechanical property and beƩer lightness value than regular PET boƩles. In some embodiments, the present invenƟon provides a method of preparing enzyme cocktail comprising the variant Bhr-PETase as described herein with other PET degrading accessory enzymes and downstream MHETase to produce a PET degrading enzyme cocktail for the efficient turn-over of pretreated PET. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 1 and has PETase acƟvity. In some embodiments, the present disclosure relates to a composiƟon comprising the amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, S27L, P20T, V23T, A24V, W69H, W69I, W69L, T109L, S110R, T157A, A174R, H183S, F187I, D203K, D203R, F208D, F208G, F208T, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, seventeen of said posiƟons, or eighteen of said posiƟons. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises S27L and L90Y. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, and P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity. In some embodiments, the present disclosure relates to a composiƟon comprising the amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, P20T, V23T, A24V, W69H, W69I, W69L, T109L, S110R, T157A, A174R, H183S, F187I, D203K, D203R, F208D, F208G, F208T, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, or seventeen of said posiƟons. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises L90Y. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, and P20T / V23T / A24V / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, P20T, V23T, A24V, W69H, A174R, T176S, V177T, H183T, D203A, D203G, D203S, F208D, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, W69F and S181D. In some embodiments, the present disclosure relates to a composiƟon comprising the variant variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203G, D203S, F208D, I222L and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, T157D, T157E, T157G, T157Q, T157S, A174G, V177I, V177W, S181A, S181Q, S181T, D203E, D203G, D203L, D203N, D203V, D203Y, N204S, S212E, S212H, S212I, P213A, A215K, A215S, A216I, A246E and A246N. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, P20T, V23T, A24V, W69H, A174R, H183T, F208D, I222L, and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, W69F, D203A and D203S. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, T176S, V177T, and S181D. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203S, F208D, I222L, and V229I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of W69I, W69L, H183S, H183Y, D203G, D203R, F208Q, and F208V. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / A246E, A174G / D203A / A216E, and L90Y / A174G. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12F / L90Y, R12T / L90A / T176S / S212E, and R12Q / T176S. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, T176S / V177T / A215D / A246E, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, and T176S / D203A / A216I. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12F / L90Y, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / A246E, A174G / D203A / A216E, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, R12Q / T176S, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, L90Y / A174G, T176S / D203A / A216I, R12T / L90A / T176S / S212E, and T176S / V177T / A215D / A246E. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr- PETase are more stable than as compared to SEQ ID NO: 1. In some embodiments, the present disclosure relates to a composiƟon comprising the variant Bhr-PETase has improved acƟvity and / or thermostability at a pH above 6 with respect to SEQ ID NO: 1. EXAMPLES Example 1: Design and ConstrucƟon of Bhr-PETase Libraries and Specific Variants Bhr-PETase G2P, which contained the S27L amino acid modificaƟon was reported in WO 2023 / 019222 A1 to exhibit significantly improved PET-degrading acƟvity over the Bhr-PETase wild type. A further variant, Bhr-PETase G3P, which contained the S27L modificaƟon, a linker, and a C- terminal domain, was developed in internal studies with enhanced performance. The G3P variant was developed by introducing a novel C-terminal domain into G2P. This C-terminal domain includes five amino acid subsƟtuƟons relaƟve to the wild-type hydrophobin HFB4 (GenBank accession no. XP_006964739.1), and was designed to improve protein expression and stability. To further enhance the protein Ɵter and PET-degrading capability of Bhr-PETase, mutagenic libraries and specific variants were designed based on experimental results as well as in-depth sequence and structural analysis. Mutagenesis and subsequent cloning of the mutated genes into suitable expression vectors were performed using established techniques. The resulƟng variants were expressed in a host organism opƟmized for Bhr-PETase variant expression. Example 2: HTP PreparaƟon of Bhr-PETase Variants in MicroƟter Plates Recombinant host strains containing Bhr-PETase variant genes were cultured from single colonies in 24-well microƟter plates containing a specific growth medium. Cultures were incubated overnight at 30°C with 200 rpm shaking and maintained at 85% relaƟve humidity. These primary cultures were then diluted and inoculated into fresh plates with idenƟcal media. The cultures underwent further incubaƟon under the same condiƟons and were induced periodically with an inducer compound over a span of up to 120 hours to maximize variant expression. Culture supernatants were subsequently decanted into round-boƩom plates for storage at -20°C, preserving protein integrity prior to analysis via various assays. Example 3: PreparaƟon of Bhr-PETase Variants in Large Scale Selected variant strains were grown in shakeflasks with baffled boƩom containing a specific growth medium. Cultures were incubated overnight at 30°C with 200 rpm shaking and maintained at 85% relaƟve humidity. These primary cultures were then diluted and inoculated into fresh shakeflasks with baffled boƩom with idenƟcal media. The cultures underwent further incubaƟon under the same condiƟons and were induced periodically with an inducer compound up to 120 hours to maximize variant expression. Selected variant strains were grown in fermenters. The transformed host organisms were cultured in a fermentaƟon medium that was tailored for the growth requirements of the specific host. Throughout the fermentaƟon process, criƟcal parameters such as temperature, pH, aeraƟon, agitaƟon, and feeding schedules were meƟculously controlled to maximize enzyme producƟon. The progress of the fermentaƟon was monitored by measuring the biomass concentraƟon, and periodic sampling was conducted to evaluate protein expression levels. In a comparaƟve evaluaƟon, the same fermentaƟon and sampling procedures were applied to Bhr- PETase G1P, G2P, and G3P variants (SEQ ID NOs: 2, 4, and 6, respecƟvely). In a comparaƟve evaluaƟon, the same fermentaƟon and sampling procedures were applied to Bhr-PETase G1P, G2P, and G3P variants (SEQ ID NOs: 1, 3, and5, respecƟvely). Protein Ɵters were measured from the clarified supernatant using the Bradford assay as described in Example 5. The results, shown in Figure 16, demonstrated that G3P achieved the highest supernatant protein Ɵter under the tested fermentaƟon condiƟons, followed by G2P and then G1P. Thesefindings suggest that the addiƟonal C- terminal domain present in G3P may contribute to improved expression or stability, supporƟng its suitability for large-scale enzyme producƟon and sustainable PET recycling applicaƟons. Example 4: HTP PET Film Assay for EvaluaƟng PET DegradaƟon Total AcƟvity and Specific AcƟvity of Bhr-PETase Variants Kirkland PET water boƩles was procured from Costco. Thefilm and boƩles were cut into 1 x 30 cm strips with a paper trimmer and further reduced to approximately 1 x 0.25 cm strips. These strips were then ground into afine powder using a mechanical grinder. In preparaƟon for the assay, a resin loader accurately dispensed approximately 10-12 mg of PET powder into each well of a 96-well Costar deep well plate. 300-700 µL of enzyme soluƟon and 1200-800 µL of 0.4 M Tris-HCl buffer (pH 9.0) were added in each well containing PET powder (total volume of soluƟon is 1.5 mL, thefinal concentraƟon of Tris-HCl: 0.32-0.21 M). The plates were sealed to minimize evaporaƟon. The enzyme-PET mixture was incubated at 65-68°C for 6-24 hours to facilitate the enzymaƟc degradaƟon of the PET. AŌer incubaƟon, the plates were centrifuged at 4,000 rpm for 2 minutes to sediment any undigested PET. A 180 µL sample of the clear supernatant was transferred into a new Costar round boƩom plate, to which 20 µL of an in-house prepared Is-MHETase enzyme soluƟon was added. This new mixture was incubated at 50°C for 30 minutes to ensure the further degradaƟon of any PET breakdown products. Post-secondary incubaƟon, the plates were again centrifuged at 4,000 rpm for 2 minutes. The supernatant was diluted with sodium phosphate buffer (pH 7.2) to a concentraƟon within the linear range of the detecƟon assay. To this diluƟon, 50 µL of 10 mM EDTA and 50 µL of 10 mM FeSO4 were introduced. The reacƟon plates were then incubated in the dark for 10 minutes to miƟgate the effects of light-sensiƟve components. Afinal centrifugaƟon step at 4,000 rpm for 2 minutes preceded the transfer of 200 µL of the reacƟon mixture to a black clear-boƩomfluorometric plate forfluorescence measurement. AŌer a 10-minute stabilizaƟon period, endpointfluorescence was recorded using an excitaƟon wavelength of 328 nm and an emission wavelength of 421 nm, which are the specific parameters for TPA (Terephthalic Acid) detecƟon. The results, illustraƟng the improved PET degradaƟon total acƟvity of all G2 variants in comparison to their parent enzyme (G2P), are documented in Figure 3. Among these variants, the one containing the S27L / L90Y amino acid modificaƟons exhibited a significant increase in PET degradaƟon total acƟvity and was consequently designated as G4P (GeneraƟon 4 Parent). To determine PET degradaƟon specific acƟvity, the PET degradaƟon total acƟvity was normalized against protein concentraƟon, which was quanƟfied using the Bradford assay. The Bradford assay is a widely used colorimetric method for protein quanƟficaƟon, based on the binding of Coomassie Brilliant Blue dye to proteins. This method enabled accurate determinaƟon of protein concentraƟon, ensuring reliable normalizaƟon of enzymaƟc acƟvity. The results, demonstraƟng the enhanced PET degradaƟon specific acƟvity of all G4 variants relaƟve to their parent enzyme (G4P), are presented in Figure 7. Example 5: Assays for EvaluaƟng Protein Titer and PET DegradaƟon Specific AcƟvity of Bhr-PETase Variants Harvest cultures were used for evaluaƟng protein Ɵter. The cultures were applied onto SDS-gels and perform SDS-PAGE. The SDS-gel pictures were taken with E-gel imager (Thermo Fisher ScienƟfic) and these pictures were used for determining protein amount by GelQuant Express Analysis SoŌware (Thermo Fisher ScienƟfic). The protein Ɵters were calculated based on the determined protein amount. Harvest cultures were concentrated with a concentrator (e.g. Vivaspin 20 [Sartorius]). If needed, the soluƟons were exchanged with 0.1 M phosphate buffer (pH 8.0). The protein concentraƟons were measured by GelQuant Express Analysis SoŌware using the SDS-gels aŌer SDS-PAGE was performed using the concentrated soluƟon. AlternaƟvely, protein concentraƟon was esƟmated using a spectrophotometer capable of measuring absorbance at 280^nm (A280). The exƟncƟon coefficient and molecular weight of each enzyme variant were determined from its amino acid sequence and used to calculate protein concentraƟon based on absorbance. This method was applied when samples were sufficiently pure and compaƟble with absorbance-based quanƟficaƟon. Amorphous PETfilm or Kirkland PET water boƩles were trimmed to approximately 1 x 0.25 cm strips and then the strips were grinded using a mechanical grinder. The PET powder (0.8 g, 180-500 um powder size), 20 mL of 0.4 M Tris-HCl buffer (pH 9.0) and 1.2 mg or 1.28 mg of enzyme were added in a glass boƩle (1.5 or 1.6 mg enzyme / g PET powder, 40 g PET powder / L reacƟon soluƟon) and then PET depolymerizaƟon reacƟon was performed at 60-68 degree. Sampling was performed at 2 hr-, 6 hr-, 8 hr- or 24 hr-incubaƟons. Depolymerized samples were analyzed by HPLC and the depolymerizaƟon rates were calculated based on sum of TPA and MHET amounts. The results, illustraƟng the improved protein Ɵter of all variants in comparison to their parent enzyme (G2P), are documented in Figures 4A-B. The results, illustraƟng the improved PET degradaƟon specific acƟvity of all variants in comparison to their parent enzyme (G2P), are documented in Figures 5A-B and Figure 6. Notably, several variants demonstrated over 90% PET depolymerizaƟon in scaled-up bioreactors at 60-68°C. It is well-established that PET degradaƟon is opƟmized at high temperatures near the glass transiƟon temperature of PET, where PET has increased mobility. However, reacƟons at such high temperatures necessitate more energy and excepƟonally thermostable PETase. UnƟl now, efforts in the literature have predominantly aimed at enhancing the thermostability of PETase. The results show that variants with novel combinaƟons can efficiently degrade PET at significantly lower temperatures, offering a transformaƟve reducƟon in energy consumpƟon for PET biodegradaƟon. This approach introduces a highly energy-efficient method for PET biodegradaƟon, presenƟng substanƟal advancements in thefield. Example 6: AI-Guided IdenƟficaƟon of Improved Bhr-PETase Variants The dataset comprising over 500 variant sequences, including their fold improvement in PET degradaƟon specific acƟvity over G4P at 6 hours, was obtained from Example 4 and next-generaƟon sequencing (NGS). This dataset was analyzed to assess performance as a funcƟon of mutaƟon rate, defined as the number of amino acid subsƟtuƟons per variant relaƟve to the G1P reference sequence. As shown in Figure 10, variants with 6 to 10 mutaƟons exhibited the highest observed fold improvements. An arƟficial intelligence–directed molecular evoluƟon (AIDME) approach was applied using the systems and methods disclosed in US Patent ApplicaƟon PublicaƟon No. US 2024 / 0257907 A1, which is incorporated herein by reference. The dataset was encoded and used to train surrogate models that predict fold improvement over G4P based on combinatorial mutaƟon profiles. Training and validaƟon sets were created using an 80 / 20 split. As shown in Figure 11, all surrogate models achieved Spearman’s rank correlaƟon coefficient (ρ) above 0.6 on the validaƟon set, with Δρ below 0.1, indicaƟng strong predicƟve performance and low overfiƫng. Figures 12 and 13 illustrate the applicaƟon and validaƟon of the predicƟve models. Figure 12 presents a correlaƟon plot between predicted and experimentally measured fold improvement across the full dataset. Figure 13 shows the top 20 variants ranked by predicted fold improvement using one surrogate model. Most of these variants exhibited experimentally confirmed improvement. In addiƟon to 6-hour acƟvity, the same predicƟve framework was also applied to esƟmate fold improvement at 24 hours and the percentage of MHET in the product mixture at 24 hours. The surrogate models were then integrated into a Bayesian opƟmizaƟon framework to idenƟfy novel combinatorially subsƟtuted variants. The search was constrained to the experimentally determined opƟmal mutaƟon range of 6 to 10 mutaƟons per variant. Only mutaƟons predicted to be beneficial or neutral were used in recombinaƟon. As shown in Figure 14, this process generated over one hundred predicted variants with significantly improved fold improvement relaƟve to G4P and even the best variants from high-throughput screening in Example 4. A representaƟve set of 100 predicted variants is listed in Figure 15. These variants were selected based on predicted fold improvement using mulƟple models, and reported values reflect the mean predicƟon across different model types. Some variants were also selected based on predicted properƟes relevant to downstream degradaƟon outcomes. The AI methodology has previously yielded experimentally validated improved variants, as disclosed in US 2024 / 0257907 A1. These results support a reasonable expectaƟon that the variants in Figure 15 are funcƟonal and improved. The disclosed models, opƟmizaƟon process, and mutaƟon constraints provide sufficient guidance for a person of ordinary skill in the art to reproduce and apply the predicƟve approach to idenƟfy addiƟonal funcƟonal variants. Example 7: Large-Scale PET Film Assay to Assess the Impact of the Engineered G3P C- Terminal PET degradaƟon experiments were conducted in 250 mL glass boƩles using amorphous PETfilm (Catalog # ES301445, Goodfellow) with a thickness of 0.25 mm. The PETfilm was cut into 1 × 30 cm strips, then into approximately 1 × 0.25 cm segments, and ground into afine powder using a mechanical grinder. PET powder was loaded into 0.1 M sodium phosphate buffer (pH 8.0) at concentraƟons ranging from 2 to 10 g / L, with afinal reacƟon volume of 50 mL. Normalized protein doses were evaluated under reacƟon condiƟons of 65°C and 200 rpm shaking for duraƟons ranging from 6 to 144 hours. At various Ɵme points, samples were collected for analysis. Terephthalic acid (TPA) concentraƟons were quanƟfied using high-performance liquid chromatography (HPLC). For HPLC analysis, a Zorbax Eclipse Plus C18 column (Rapid ResoluƟon HD, 2.1 × 50 mm, 1.8 µm; Part No.959757-902) with a guard column (Part No.82175-901) was used. Theflow rate was 0.6 mL / min, with the column maintained at 35°C. A gradient method was employed using mobile phase A (water + 0.1% trifluoroaceƟc acid) and mobile phase B (acetonitrile + 0.1% trifluoroaceƟc acid) for TPA detecƟon. As shown in Figure 17, the engineered G3P C-terminal domain, when covalently linked via aflexible linker to G1P or G2P, resulted in enhanced % conversion of PET to TPA. These results support that the engineered C-terminal domain improves PET degradaƟon efficiency relaƟve to the wild-type HFB4 hydrophobin domain. Example 8: Design and ConstrucƟon of Bhr-PETase G3P-Derived Variant Libraries To enhance PET-degrading efficacy, protein Ɵter, and specific acƟvity, diverse variant libraries were designed based on in-depth sequence and structural analysis. These libraries introduced modificaƟons ranging from single to mulƟple amino acid subsƟtuƟons into the GeneraƟon 3 Parent (G3P), which contains the S27L amino acid modificaƟon, a linker and an engineered C-terminal PET- binding domain. Two types of libraries were generated. Thefirst used an untagged G3P parent and was screened primarily based on PET degradaƟon acƟvity. The second was constructed using a GFP-tagged version of G3P to enablefluorescence-based quanƟficaƟon of protein expression. In the tagged library, the GFP fusion allowed concurrent assessment of acƟvity and expression level, which enabled subsequent calculaƟon of specific acƟvity. Mutagenesis and cloning into suitable expression vectors were performed using established techniques. Both libraries were expressed in a host organism opƟmized for high-throughput screening. This approach enabled parallel idenƟficaƟon of high-performing variants across different performance metrics, with tagged variants allowing more comprehensive characterizaƟon. Example 9: High-Throughput PET Film Assay for EvaluaƟng Total AcƟvity of Bhr- PETase G3P-Derived Variants Amorphous PETfilm (0.25 mm thickness, Catalog # ES301445) was procured from Goodfellow. The film was cut into 1 × 30 cm strips, further reduced to approximately 1 × 0.25 cm segments, and ground into afine powder using a mechanical grinder. Bhr-PETase G3P-derived variants from both untagged and GFP-tagged libraries were cultured in 24-well plates under the same condiƟons described in Example 2, with culture supernatants collected and stored for subsequent analysis. Approximately 10–12 mg of PET powder was dispensed into each well of a 96-well Costar deep-well plate using a resin loader. To each well, 300–700 µL of enzyme soluƟon and 1200–800 µL of 0.4 M Tris-HCl buffer (pH 9.0) were added (total volume: 1.5 mL;final Tris-HCl concentraƟon: 0.21–0.32 M). The sealed plates were incubated at 65°C for 20–24 hours to facilitate enzymaƟc degradaƟon of the PET. AŌer incubaƟon, plates were centrifuged at 4,000 rpm for 2 minutes. A 180 µL aliquot of supernatant was transferred into a new round-boƩom plate and treated with 20 µL of in-house Is-MHETase enzyme soluƟon. AŌer 30 minutes of incubaƟon at 50°C, the plates were centrifuged again, and supernatants were diluted with sodium phosphate buffer (pH 7.2) to bring concentraƟons within the linear detecƟon range. To each diluted sample, 50 µL of 10 mM EDTA and 50 µL of 10 mM FeSO₄ were added. AŌer a 10- minute dark incubaƟon to minimize light interference, the mixture was centrifuged again and 200 µL was transferred to a black, clear-boƩomfluorometric plate. Fluorescence was measured using excitaƟon at 328 nm and emission at 421 nm to quanƟfy TPA formaƟon. Figure 18 summarizes the PET degradaƟon total acƟvity results obtained from both library types under these assay condiƟons. Example 10: High-Throughput Fluorescence Assay for EvaluaƟng Protein Titer and PET DegradaƟon Specific AcƟvity of GFP-Tagged Bhr-PETase G3P-Derived Variants GFP-tagged Bhr-PETase G3P-derived variants were cultured in 24-well plates under the same condiƟons described in Example 2, with culture supernatants collected and analyzed for protein Ɵter and specific acƟvity. Variants containing GFP tags were analyzed using a plate reader configured to detect GFPfluorescence at its characterisƟc excitaƟon and emission wavelengths. GFP-only controls were included to correct for background signal. Fluorescence intensity was used to esƟmate relaƟve protein producƟon for each GFP-tagged variant. These values were used to normalize PET degradaƟon acƟvity measurements (obtained in parallel assays) in order to calculate the specific acƟvity of each variant. Figure 18 also presents the protein Ɵter and specific acƟvity data obtained from the GFP-tagged variant library. The results demonstrate that individual variants exhibit enhanced performance in one or more key metrics—protein Ɵter, PET degradaƟon acƟvity, and / or specific acƟvity—relaƟve to the parent G3P enzyme. Example 11: Shake Flask EvaluaƟon of AI- and RaƟonally Designed G1P-Derived Variants To validate the funcƟonal performance of computaƟonally designed Bhr-PETase variants under shake flask condiƟons, several G1P-derived variants—specifically G4P-derived variants—were constructed and evaluated. Six variants (AI Variants 1-6) were selected from the predicted variant set generated in Example 6 using the arƟficial intelligence–directed molecular evoluƟon (AIDME) framework. These variants included between 6 and 10 subsƟtuƟons per sequence, consistent with the model’s opƟmal mutaƟon range for funcƟonal improvement. An addiƟonal variant, RaƟonal Variant 7, was designed through a complementary raƟonal design approach, informed by structural modeling and prior AI- guided insights into beneficial subsƟtuƟons. All seven AI or RaƟonal Variants, along with two benchmark variants (ICL00065494 and ICL00068032) previously idenƟfied through experimental screening (Example 4), were assembled as described in Example 1, expressed under shakeflask condiƟons according to Example 3, and evaluated using the PET degradaƟon acƟvity assay described in Example 5. Protein concentraƟons were quanƟfied by SDS-PAGE with GelQuant Express analysis or exƟncƟon coefficient analysis, and enzyme doses were normalized across all samples to enable direct comparison under matched condiƟons. The results, summarized in Figure 20A show the fold improvement in PET degradaƟon specific acƟvity for each variant at the 18-19 hour Ɵme point, relaƟve to the G4P reference enzyme. All AI Variants 1-6 and RaƟonal Variant 7 outperformed both G4P and the two top-performing G4 variants from Example 4 (ICL00065494 and ICL00068032), each of which demonstrated a 1.14-fold improvement. Among the AI and RaƟonal Variants: • AI Variants 3 and 4 exhibited the highest improvements (1.39-fold), • AI Variants 5 and 6 followed closely (1.33-fold and 1.31-fold, respecƟvely), • AI Variant 2 and RaƟonal Variant 7 each showed a 1.26-fold improvement, • AI Variant 1 showed a 1.11-fold improvement. Figure 20B presents both predicted and measured fold improvement at early stages of the reacƟon. Predicted values reflect PET degradaƟon specific acƟvity at 6 hours based on high-throughput screening results and AIDME modeling. RaƟonal Variant 7 does not have a predicted value, as it was not generated by the AIDME model but was instead designed independently through a raƟonal design approach. Measured values were obtained at 3 hours in shakeflask assays to capture acƟvity within the linear range prior to conversion saturaƟon. All AI Variants 1-6 and RaƟonal Variant 7 exhibited strong early-stage performance, with several variants showing more than 2.5-fold improvement over G4P. Thesefindings are consistent with the model predicƟons described in Example 6 and confirm that both AIDME-guided and raƟonally designed variants can produce funcƟonally enhanced G1P-derived enzymes in scaled-up assay formats. The successful performance of these variants further supports the predicƟve power and pracƟcal uƟlity of computaƟonal design strategies in engineering PET- degrading enzymes.

Claims

AƩorney Docket No.129436-5004-WO CLAIMS 1. A composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 8, 12, 16, 20, 23, 24, 69, 89, 92, 109, 110, 112, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 212, 213, 215, 216, 218, 222, 223, 229, 246, 248 and 249, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO:1 and has PETase acƟvity.

2. The composiƟon according to claim 1, wherein said composiƟon comprises a variant Bhr- PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 12, 20, 23, 24, 69, 89, 92, 109, 110, 157, 170, 174, 176, 177, 181, 182, 183, 187, 203, 206, 208, 212, 215, 216, 222, 229, 246 and 249.

3. The composiƟon according to claim 1 or 2, wherein said composiƟon comprises a variant Bhr-PETase as compared to SEQ ID NO:1, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 27, 8, 16, 112, 173, 204, 213, 218, 223 and 248.

4. The composiƟon according to any one of the preceding claims, wherein said variant comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 20, 23, 24, 69, 89, 92, 109, 110, 157, 170, 174, 176, 177, 181, 182, 183, 187, 203, 206, 208, 212, 215, 216, 222, 229, 246 and 249.

5. The composiƟon according to any one of the preceding claims, wherein said variant comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 8, 16, 112, 173, 204, 213, 218, 223 and 248.

6. The composiƟon according to any one of the preceding claims, wherein said variant comprises a subsƟtuƟon at posiƟons 27 and 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 1 at an amino acid posiƟon(s) selected from the group consisƟng of 8, 12, 16, 20, 23, 24, 69, 89, 92, 109, 110, 112, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 212, 213, 215, 216, 218, 222, 223, 229, 246, 248 and 249.

7. The composiƟon according to any one of the preceding claims, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, S27L, R12E, P20T, V23T, A24V, W69H, W69I, W69L, W69S, R89K, R89M, L90A, F92G, T109L, S110R, T157A, T157G, T157L, T157Q, V170I, V170L, A174G, A174R, T176S, V177I, V177T, S181T, Q182E, H183S, H183T, F187I, F187L, D203A,D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, T206D, F208D, F208G, F208S, F208T, S212Q, A215D, A216D, A216E, A216T, I222L, V229I, A246Q and D249L.

8. The composiƟon according to any one of the preceding claims, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of R12T, W69C, W69F, W69G, T157D, A174K, S181D, H183A, H183D, H183Y, F187V, F208I, F208K, F208Q, F208V and S212N.

9. The composiƟon according to any one of the preceding claims, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of P8L, T16A, P112Q, E173N, E173P, E173T, E173V, E173W, N204G, P213D, S218G, S218R, S223N and S248R.

10. The composiƟon according to any one of the preceding claims, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, S27L, P8L, R12E, R12T, T16A, P20T, V23T, A24V, W69C, W69F, W69G, W69H, W69I, W69L, W69S, R89K, R89M, L90A, F92G, T109L, S110R, P112Q, T157A, T157D, T157G, T157L, T157Q, V170I, V170L, E173N, E173P, E173T, E173V, E173W, A174G, A174K, A174R, T176S, V177I, V177T, S181D, S181T, Q182E, H183A, H183D, H183S, H183T, H183Y, F187I, F187L, F187V, D203A, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, T206D, F208D, F208G, F208I, F208K, F208Q, F208S, F208T, F208V, S212N, S212Q, P213D, A215D, A216D, A216E, A216T, S218G, S218R, I222L, S223N, V229I, A246Q, S248R and D249L.

11. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, P20T, V23T, A24V, W69H, A174R, T176S, V177T, H183T, D203A, D203G, D203S, F208D, I222L and V229I.

12. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, W69F and S181D.

13. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons of S27L, L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203G, D203S, F208D, I222L and V229I.

14. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, seventeen of said posiƟons, eighteen of said posiƟons, nineteen of said posiƟons or twenty of said posiƟons.

15. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of S27L / L90Y / A174G / D203V / D249L, S27L / A174G / D203L / D249L, S27L / L90A / A174G / D203R / D249L,S27L / L90Y / A174G / D203N, S27L / L90Y / A174G / D249L, S27L / L90Y / A174G / D203G / D249L, S27L / L90Y / D203L / D249L, S27L / D203L / D249L, S27L / L90A / A174G / D203A / D249L, S27L / L90A / A174G / D203Y, S27L / L90Y / A174G / D203A / D249L, S27L / L90Y / A174G / D203L / D249L, S27L / L90Y / D203A / D249L, S27L / L90Y / D203Y / D249L, S27L / L90Y / D203K / D249L, S27L / L90Y / D249L, S27L / A174G / D249L, S27L / L90Y / A174G / D203K / D249L, S27L / L90Y / A174G / D203Y / D249L, S27L / L90Y, S27L / I222L, S27L / I222L / V229I, P20T / V23T / A24V / S27L / T109L / I222L / V229I, P20T / V23T / S27L / I222L / V229I, P20T / S27L / T109L / I222L / V229I, P20T / A24V / S27L / I222L / V229I, P20T / V23T / S27L / T109L / S110R / I222L / V229I, A24V / S27L / I222L / V229I, P20T / V23T / A24V / S27L / S110R / I222L / V229I, S27L / T109L / I222L, P20T / S27L / I222L / V229I, S27L / T109L / I222L / V229I, P20T / V23T / A24V / S27L / T109L / I222L, A24V / S27L / T109L / I222L / V229I, P20T / S27L, P20T / V23T / S27L / S110R / I222L / V229I, R12E / S27L / D203R, S27L / F92G / T157Q / V170I, S27L / R89M / T157G / S181T / Q182E, S27L / T157Q / V170L / T176S / V177T / A215D / A216T, V23T / A24V / S27L / T109L / S110R / I222L / V229I, P20T / A24V / S27L / T109L / S110R / I222L / V229I, P20T / V23T / A24V / S27L / T109L / S110R / V229I, P20T / V23T / A24V / S27L / T109L / S110R / I222L, S27L / T206D, S27L / V177I / A215D / A216T, S27L / R89K / V170I / T176S / V177I / D203S, S27L / A215D, S27L / T157G / V170I / Q182E / D203V, S27L / V170I / T206D / S212Q, P20T / V23T / A24V / S27L / L90Y / T109L / S110R / I222L / V229I, R12E / P20T / V23T / A24V / S27L / T109L / S110R / D203R / I222L / V229I, R12E / P20T / V23T / A24V / S27L / L90Y / T109L / S110R / D203R / I222L / V229I, R12E / S27L / L90Y / D203R, P20T / V23T / A24V / S27L / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69S / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / T109L / S110R / A174R / H183S / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A 246Q, P20T / V23T / A24V / S27L / W69I / L90Y / T109L / S110R / T157L / H183T / F187L / D203R / F208S / A216D / I222L / V 229I / A246Q, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216E / I222L / V229I / A246Q,S27L / V177I / A216T, A24V / S27L / I222L, S27L / D203R, P20T / V23T / A24V / S27L / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216T / I222L / V229I / A246Q, S27L / L90Y / A174G / D203V / D249L, S27L / A174G / D203L / D249L, S27L / L90A / A174G / D203R / D249L, S27L / L90Y / A174G / D203N, S27L / L90Y / A174G / D249L, S27L / L90Y / A174G / D203G / D249L, S27L / L90Y / D203L / D249L, S27L / D203L / D249L, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, P20T / V23T / A24V / S27L / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I, S27L / W69H / H183S / F187I / D203K / F208G, S27L / W69H / L90Y / H183S / F187I / D203K / F208G, S27L / W69H / H183S / F187I / D203R / F208G, S27L / W69H / L90Y / H183S / F187I / D203R / F208G, S27L / W69H / L90Y / A174R / H183S / D203R / F208D, and S27L / W69H / L90Y / T157A / A174R / H183S / D203R / F208D.

16. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of P20T / S27L / L90Y / I222L / V229I, V23T / A24V / S27L / L90Y / T176S / A215D, A24V / S27L / W69S / L90Y / T109L / H183T / D203R / A215D / I222L / V229I, S27L / L90Y / H183S / D203K, P20T / S27L / L90Y / T109L / D203R / F208D / I222L / V229I, S27L / L90Y / T109L / H183S, A24V / S27L / L90Y / D203K, S27L / L90Y / H183S / I222L / V229I, S27L / W69L / L90Y / H183S, S27L / L90Y / T176S / V177T, S27L / L90Y / T176S / V229I, A24V / S27L / L90Y, S27L / W69H / L90Y / A215D, A24V / S27L / L90Y / T109L / H183S / F208D, P20T / A24V / S27L / L90Y / T109L / H183S / I222L / V229I, S27L / W69L / L90Y / H183S / D203K / F208D / I222L / V229I, P20T / S27L / L90Y / D203K / F208D / I222L / V229I, A24V / S27L / L90Y / H183S / F208D / I222L / V229I, S27L / L90Y / D203R / F208D / I222L / V229I, S27L / L90Y / F208D / V229I, A24V / S27L / W69I / L90Y / H183S / D203R, S27L / W69I / L90Y / T109L / H183S / D203R / I222L / V229I, A24V / S27L / W69I / L90Y / D203K / F208D / I222L / V229I, A24V / S27L / W69L / L90Y, S27L / L90Y / D203K / I222L / V229I, A24V / S27L / L90Y / T109L / H183S / D203R / F208D, A24V / S27L / L90Y / A174R / D203K, S27L / L90Y / D203A / A215D, S27L / L90Y / I222L / V229I, S27L / W69H / L90Y / T176S / V177T / A215D, P20T / A24V / S27L / L90Y / S212Q / V229I, V23T / S27L / L90Y / T176S / V177T / D203G / A215D, S27L / L90Y / D203S / A215D / I222L / V229I, S27L / L90Y / T176S / F208D / A215D, V23T / A24V / S27L / W69H / L90Y / V177T / D203S, S27L / L90Y / V177T, S27L / L90Y / V229I, S27L / L90Y / T176S / D203S / A215D, S27L / L90Y / S212Q,S27L / L90Y / D203R / A215D / V229I, S27L / L90Y / S212Q / I222L / V229I, V23T / A24V / S27L / L90Y, P20T / S27L / L90Y / S110R / A174R / T176S / A215D / I222L / V229I, S27L / L90Y / T109L / A174R / A215D, S27L / L90Y / T109L / D203G / A215D, P20T / S27L / L90Y / S110R / D203G / A215D / I222L, S27L / L90Y / A215D, V23T / S27L / L90Y / T109L / T157A / D203R / V229I, S27L / L90Y / T176S, S27L / L90Y / D203G, V23T / A24V / S27L / L90Y / V229I, V23T / A24V / S27L / L90Y / H183T / I222L / V229I, V23T / A24V / S27L / W69L / L90Y / V177T / A215D, S27L / L90Y / A174R / H183T / D203K / S212Q, V23T / A24V / S27L / L90Y / S110R / T176S / F208D, S27L / L90Y / D203A, S27L / L90Y / F208D, P20T / S27L / L90Y / T176S / F208D / A215D, S27L / L90Y / T109L / T176S / I222L, S27L / W69L / L90Y / S212Q, S27L / L90Y / A215D / V229I, P20T / S27L / L90Y / V229I, V23T / S27L / L90Y / V229I, S27L / W69L / L90Y V23T / A24V / S27L / L90Y / T109L / T176S / D203R / F208D, and S27L / L90Y / A215D / I222L / V229I.

17. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of S27L / L90Y / T176S / F187V / D203G / F208K / V229I, S27L / W69C / L90Y / T109L / S181D / H183T / D203A / F208K / I222L / V229I, S27L / W69L / L90Y / D203G / F208Q, S27L / L90Y / T109L / A174K / S181D / F208D, V23T / A24V / S27L / L90Y / A174R / S181D / H183S / F208D / A215D, P20T / S27L / W69G / L90Y / A174R / S181D / H183A / F187V / D203K / S212Q, V23T / S27L / L90Y / T176S / F208Q / A215D, A24V / S27L / L90Y / T109L / A174K / D203K / F208D / V229I, P20T / S27L / L90Y / T157A / D203K / F208V / V229I, A24V / S27L / W69L / L90Y / F187V / D203R / F208V / I222L / V229I, A24V / S27L / W69L / L90Y / T109L / A174K / D203K / F208V, S27L / L90Y / T109L / H183S / F187V, A24V / S27L / L90Y / A174K / F187V / F208D / V229I, 24V / S27L / L90Y / A174K / H183S / F187V / D203R / I222L / V229I, P20T / S27L / L90Y / H183S / D203R / F208V / V229I, S27L / L90Y / A174R / H183S / F187V / D203R, A24V / S27L / L90Y / T109L / A174R / H183S / F187V / D203R / F208D / I222L / V229I, P20T / S27L / L90Y / F208V, S27L / L90Y / H183T / D203S / F208K, P20T / V23T / S27L / L90Y / D203G / F208Q, S27L / L90Y / D203G / F208Q, V23T / A24V / S27L / L90Y / T176S / H183T / D203K / F208Q / A215D / I222L / V229I, S27L / L90Y / A174K / S181D / H183Y / D203R / F208V, V23T / A24V / S27L / L90Y / A174K / T176S / S181D / H183S, S27L / W69G / L90Y / H183Y / F187V / D203S, P20T / S27L / L90Y / T157A / S181D / H183S / D203S / A215D / I222L / V229I, S27L / W69S / L90Y / T109L / H183D / F187V / D203R / F208Q / A215D / I222L / V229I, A24V / S27L / L90Y / F208Q / A215D / V229I, V23T / S27L / L90Y / S181D / H183A / D203G / F208V / A215D / V229I, S27L / L90Y / A174R / F187V / D203G / A215D, P20T / S27L / L90Y / S181D / H183S / F187V / F208V / A215D / I222L / V229I, S27L / W69C / L90Y / A174R / H183T / F187V / A215D / I222L, S27L / L90Y / F208V / A215D / I222L / V229I,A24V / S27L / W69H / L90Y / A174R / F208I, S27L / L90Y / F208V, A24V / S27L / W69I / L90Y / A174R / H183S / F187V / D203R / F208D / I222L, A24V / S27L / L90Y / T109L / F187V / F208D, S27L / L90Y / H183S / D203R / F208V / I222L / V229I, S27L / L90Y / A174K / H183S / F208D / I222L / V229I, S27L / W69H / L90Y / A174K / H183S / D203R / F208V / V229I, S27L / L90Y / H183S / F187V / D203K, A24V / S27L / L90Y / T109L / D203K / F208V, A24V / S27L / L90Y / F187V / F208V / V229I, A24V / S27L / W69I / L90Y / A174K / H183S / F187V / D203K / F208D, S27L / L90Y / A174R / H183S / F187V / V229I, A24V / S27L / L90Y / T109L / A174K / H183S / D203K / F208D / V229I, S27L / W69H / L90Y / T109L / A174K / H183S / D203K / F208D / I222L / V229I, S27L / W69H / L90Y / H183S / F208V / I222L, A24V / S27L / L90Y / F187V / D203K / F208D, S27L / L90Y / D203R / F208V / I222L / V229I, S27L / L90Y / A174K / T176S / F208D / A215D / I222L / V229I, V23T / A24V / S27L / W69H / L90Y / T109L / A174K / D203G / F208V / V229I, V23T / S27L / L90Y / T109L / S110R / D203K / F208V / I222L / V229I, S27L / L90Y / T176S / H183Y / F187V, V23T / S27L / W69G / L90Y / T176S / V177T, V23T / S27L / W69C / L90Y / S110R / T176S / F187V / D203K, A24V / S27L / L90Y / S181D, S27L / W69I / L90Y / T157D / V177T / H183Y / F187V / I222L / V229I, A24V / S27L / L90Y / V177T / H183Y / F187V / D203G / A215D / I222L / V229I, S27L / L90Y / T176S / F208V / A215D / I222L, S27L / L90Y / A174R / H183Y / F187V / D203S / F208Q / I222L / V229I, V23T / S27L / W69C / L90Y / T176S / D203G / A215D, P20T / S27L / L90Y / T176S / S181D / H183T, V23T / S27L / L90Y / A174K / F208Q / I222L / V229I, S27L / L90Y / A174K / S181D / D203K / A215D / I222L, A24V / S27L / L90Y / T109L / A174K / S181D, A24V / S27L / W69L / L90Y / T109L / S181D / F187V / I222L / V229I, S27L / L90Y / A174K / D203G / F208Q, S27L / L90Y / S110R / D203S / F208Q, S27L / W69L / L90Y / T176S / V177T / F187V / D203S / F208D, V23T / S27L / L90Y / A174R / S181D / H183Y / F187V / D203K / F208V, A24V / S27L / L90Y / S110R / H183T / F187V / A215D, P20T / S27L / W69G / L90Y / F208D / A215D, P20T / S27L / L90Y / S110R / A174K / H183Y / F208V / A215D, S27L / L90Y / S181D / H183S / A215D / I222L / V229I, A24V / S27L / L90Y / F208Q, S27L / W69L / L90Y / T157A / A174R / H183Y / I222L / V229I, V23T / S27L / L90Y / T109L / T157D / A174K / T176S / H183Y / A215D / I222L / V229I, S27L / L90Y / T176S / V177T / F208V / A215D, V23T / A24V / S27L / W69C / L90Y / S110R / A174R / T176S / F187V / V229I, V23T / A24V / S27L / W69L / L90Y / A174R / S181D / H183Y / F187V / D203A / A215D / I222L, P20T / S27L / L90Y / T109L / T176S / V177T / F187V / I222L, S27L / L90Y / T109L / S110R / A174K / T176S, P20T / S27L / L90Y / V177T / D203A / F208Q / V229I, S27L / L90Y / T157L / T176S / F208Q / A215D, V23T / A24V / S27L / L90Y / S110R / T176S / H183A / D203A / F208Q / I222L / V229I, S27L / W69H / L90Y / T109L / S110R / H183T / D203K / F208V, A24V / S27L / W69C / L90Y / S212N / A215D, S27L / W69H / L90Y / A174R / H183T / F187V / D203R / F208V / S212Q, S27L / L90Y / A174K / F208D,27L / L90Y / A174K, S27L / L90Y / T176S / V177T / D203R / F208V / I222L / V229I, V23T / A24V / S27L / L90Y / A174K / S181D / H183Y / F187V / I222L / V229I, P20T / S27L / W69H / L90Y / A174R / H183A / A215D, S27L / L90Y / D203S / F208K, S27L / W69H / L90Y / T109L / S110R / A174R / T176S / S181D / F187V / D203A / F208Q, S27L / W69S / L90Y / A174R / S181D / H183T / F187V / D203G / F208D, A24V / S27L / W69C / L90Y / S110R / T157A / T176S / V177T / D203A / S212Q / V229I, S27L / L90Y / S181D / A215D / V229I, P20T / S27L / W69I / L90Y / V177T / S181D / H183Y, V23T / A24V / S27L / L90Y / T109L / S110R / S181D / H183S / D203A / V229I, S27L / W69G / L90Y / T109L / A174R / A215D, S27L / W69G / L90Y, S27L / L90Y / T176S / V177T / H183A / D203K / S212Q / V229I, V23T / A24V / S27L / W69C / L90Y / T109L, V23T / S27L / L90Y / A174K / T176S, S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208V, S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / A215D / V229I, S27L / W69I / L90Y / T176S / S181D / H183S / D203A / F208V / V229I, S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203S / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V, R12T / S27L / W69I / L90Y / T176S / S181D / H183S / D203S / F208V, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / S212Q, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / D203G / F208V, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / A215D / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183S / F208V / V229I, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / A215D / V229I, R12T / S27L / L90Y / T176S / S181D / H183Y / D203S / F208V / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / S212Q / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / D203G / F208V, S27L / W69I / L90Y / S181D / H183S / D203A / F208V / S212Q / A215D,S27L / W69I / L90Y / T176S / S181D / H183Y / D203A / F208Q / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / F208V / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / D203A / F208V / V229I, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / S212Q, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / D203A / F208Q, R12T / S27L / L90Y / T176S / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / T176S / S181D / H183T / D203S / F208D / V229I, S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / V177T / S181D / H183T / D203S / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / S181D / H183T / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / D203A / F208D / V229I, V23T / S27L / L90Y / T176S / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / S181D / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / S181D / D203A / F208D / V229I, R12T / S27L / L90Y / T176S / V177T / S181D / D203S / F208D / V229I, R12T / S27L / W69H / L90Y / V177T / S181D / H183T / F208D / V229I, S27L / L90Y / T176S / V177T / S181D / H183T / D203A / F208D, V23T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / H183T / D203A / F208D / V229I, S27L / L90Y / T176S / S181D / H183T / D203S / F208D / I222L / V229I, R12T / S27L / L90Y / V177T / S181D / H183T / D203A / F208D, R12T / S27L / L90Y / V177T / S181D / H183T / D203G / F208D / V229I, R12T / S27L / W69H / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / S27L / L90Y / V177T / S181D / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / V177T / S181D / D203A / F208D / V229I, R12T / S27L / W69H / L90Y / S181D / D203A / F208D / I222L / V229I, R12T / S27L / W69H / L90Y / V177T / H183T / D203A / F208D / V229I,R12T / S27L / W69H / L90Y / T176S / S181D / D203A / F208D / I222L, R12T / S27L / L90Y / T176S / H183T / D203A / F208D / V229I, R12T / S27L / L90Y / A174R / T176S / H183T / F208D / I222L / V229I, R12T / P20T / S27L / L90Y / H183S / I222L / V229I, R12T / S27L / L90Y / D203A / F208D, R12T / S27L / L90Y / A174R / T176S / H183Y / F208Q / I222L / V229I, R12T / V23T / A24V / S27L / W69S / L90Y / A174R / T176S / H183T / D203A / V229I, R12T / A24V / S27L / L90Y / T109L / H183Y / D203R / A215D / I222L / V229I, R12T / P20T / S27L / W69L / L90Y / S181D / D203R / F208V / I222L / V229I, R12T / P20T / S27L / L90Y / A174K / T176S / D203G / F208Q / A215D / I222L / V229I, R12T / S27L / L90Y / H183S / A215D / I222L, R12T / S27L / L90Y / T176S / V177T / D203S / A215D, R12T / V23T / S27L / L90Y / H183T / D203S / F208K / A215D, R12T / A24V / S27L / W69S / L90Y / T176S / D203R / I222L / V229I, R12T / V23T / S27L / L90Y / F208Q / A215D / I222L / V229I, R12T / A24V / S27L / L90Y / V177T / A215D / I222L / V229I, R12T / S27L / L90Y / A174R, R12T / S27L / L90Y / A215D / V229I, R12T / P20T / S27L / L90Y / D203K / A215D / V229I, R12T / A24V / S27L / L90Y / T176S, R12T / P20T / S27L / W69H / L90Y / T109L / S110R / A174R / H183Y / A215D, R12T / S27L / L90Y / T176S / A215D / I222L, R12T / S27L / L90Y, R12T / P20T / S27L / L90Y / H183A / F208K, R12T / V23T / A24V / S27L / L90Y / T176S / V229I, R12T / S27L / W69G / L90Y / D203K, R12T / A24V / S27L / L90Y / T109L / A215D, R12T / V23T / S27L / L90Y / T176S / H183Y / V229I, R12T / S27L / W69F / L90Y / T176S / V177T / S181D / H183T / F208D, and R12T / S27L / W69F / L90Y / S181D / D203A / F208D / I222L / V229I.

18. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of V23T / A24V / S27L / L90Y / A174K / T176S / F208Q / A215D / I222L / V229I / S248R, R12T / S27L / L90Y / E173N / A174R / A215D, V23T / A24V / S27L / L90Y / N204G / F208V / S212Q / P213D / I222L / V229I, A24V / S27L / L90Y / D203R / F208V / P213D / V229I, R12T / P20T / S27L / L90Y / A174R / T176S / S181D / H183Y / F187V / D203G / F208Q / S248R, R12T / S27L / L90Y / H183D / D203G / F208K / A215D / I222L / V229I / S248R, S27L / L90Y / D203S / N204G / F208V / I222L / V229I, R12T / S27L / L90Y / H183T / N204G / A215D, R12T / P20T / S27L / L90Y / D203R / F208V / P213D / I222L / V229I, P20T / V23T / A24V / S27L / W69C / L90Y / T109L / F187V / D203A / N204G / I222L / V229I, S27L / L90Y / E173T / A174K / S181D / H183Y / A215D / I222L / V229I / S248R, V23T / S27L / W69H / L90Y / S212Q / P213D / I222L / V229I, R12T / S27L / L90Y / D203K / N204G,P20T / S27L / L90Y / S181D / H183A / F208Q / I222L / V229I / S248R, A24V / S27L / L90Y / T109L / A174R / H183A / F208Q / A215D / V229I / S248R, S27L / L90Y / F208K / A215D / V229I / S248R, V23T / A24V / S27L / L90Y / E173T / A174R / S181D / H183S / F208Q / A215D / I222L / V229I, A24V / S27L / W69I / L90Y / A174K / T176S / D203S / F208K / P213D / I222L / V229I, R12T / S27L / L90Y / T109L / E173T / A174R / S181D / H183Y, R12T / A24V / S27L / L90Y / A174R / T176S / I222L / V229I / S248R, R12T / S27L / W69I / L90Y / T176S / V177T / P213D / I222L / V229I, S27L / L90Y / E173V / A174K / D203A / F208Q / A215D, V23T / S27L / L90Y / T176S / D203S / N204G / A215D, S27L / L90Y / F208K / A215D / S248R, A24V / S27L / W69C / L90Y / T109L / V177T / D203K / S212Q / P213D, A24V / S27L / L90Y / E173N / A174K / S181D / A215D / I222L / V229I, R12T / P20T / A24V / S27L / W69I / L90Y / H183Y / D203S / F208Q / S248R, V23T / A24V / S27L / L90Y / E173W / A174K / S181D / F187V / F208Q / S248R, S27L / L90Y / F208D / S248R, S27L / L90Y / T109L / S110R / S181D / N204G / F208K / A215D / S248R, P20T / S27L / W69L / L90Y / A174K / S181D / H183T / N204G / F208K / A215D / S248R, S27L / L90Y / A174K / T176S / F208D / I222L / V229I / S248R, S27L / L90Y / E173W / A174R / S212Q / P213D / I222L / V229I, S27L / L90Y / D203R / P213D, V23T / A24V / S27L / L90Y / F187V / F208Q / P213D / I222L / V229I, A24V / S27L / W69H / L90Y / T157A / E173N / S181D / F187V / F208K / I222L / V229I, S27L / L90Y / S110R / E173V / A174K / H183T / F187V / D203G / F208Q / A215D / I222L / V229I, V23T / A24V / S27L / W69S / L90Y / F208V / I222L / V229I / S248R, S27L / L90Y / A174K / S181D / D203K / N204G / F208Q, S27L / L90Y / V177T / S212Q / P213D / V229I, S27L / L90Y / T109L / H183Y / D203S / N204G / F208K / I222L / V229I, S27L / L90Y / T109L / S110R / S181D / H183T / D203K / F208D / P213D / S248R, R12T / V23T / A24V / S27L / L90Y / D203S / N204G / F208Q / A215D, S27L / W69I / L90Y / T109L / S212Q / P213D / I222L / S248R, S27L / L90Y / E173V / A174R / H183A / A215D / S248R, S27L / L90Y / E173V / A174R / F208K / V229I, S27L / L90Y / S110R / N204G / F208Q, S27L / L90Y / T176S / F187V / S212Q / S248R, S27L / L90Y / E173N / A174R, S27L / L90Y / D203R / N204G / F208V / A215D / I222L / V229I, S27L / L90Y / E173N / A174R / F187V, R12T / A24V / S27L / W69S / L90Y / T176S / S181D / D203G / N204G / S212Q / P213D / I222L / V229I, S27L / L90Y / T109L / S110R / A174R / F187V / D203G / F208V / S248R, S27L / L90Y / S110R / H183S / D203A / F208Q / S248R, R12T / A24V / S27L / L90Y / E173V / A174K / D203R / N204G / F208K / P213D / I222L / V229I, S27L / L90Y / T176S / D203G / N204G / F208D,R12T / V23T / S27L / W69C / L90Y / D203K / A215D / I222L / V229I / S248R, S27L / L90Y / T176S / V177T / N204G / A215D, S27L / L90Y / T176S / D203S / F208D / P213D / I222L / V229I, P20T / S27L / L90Y / T176S / P213D, R12T / S27L / L90Y / E173P / S181D / D203A / F208Q / I222L / V229I / S248R, V23T / A24V / S27L / L90Y / E173V / A174R / V177T / I222L / V229I, V23T / S27L / W69L / L90Y / F208Q / S248R, S27L / L90Y / A174K / F208Q / A215D / S248R, A24V / S27L / L90Y / D203R / F208K / A215D / I222L / V229I / S248R, S27L / W69C / L90Y / S110R / D203G / N204G / S212Q / V229I / S248R, V23T / S27L / L90Y / A174R / S181D / H183Y / F187V / F208D / P213D / S248R, R12T / V23T / S27L / W69S / L90Y / T109L / E173N / A174R / H183Y / A215D / S248R, R12T / S27L / W69L / L90Y / E173T / A174R / S181D / H183Y / A215D / V229I, R12T / S27L / L90Y / A215D / I222L / S248R, V23T / A24V / S27L / L90Y / A174R / T176S / H183A / D203A / N204G / A215D, S27L / L90Y / E173N / S181D / H183A / I222L / V229I / S248R, S27L / L90Y / V229I / S248R, S27L / L90Y / P213D / I222L, A24V / S27L / W69S / L90Y / E173N / A174R / F208D / A215D / V229I, V23T / A24V / S27L / L90Y / S110R / T176S / D203G / N204G / F208K / A215D / I222L / V229I, S27L / L90Y / N204G / F208D / I222L / V229I, R12T / A24V / S27L / L90Y / D203A / N204G / F208Q / A215D / I222L / V229I, V23T / A24V / S27L / L90Y / T109L / E173T / H183T, S27L / L90Y / E173T / D203K / F208D / I222L / V229I, R12T / V23T / A24V / S27L / L90Y / E173T / A174R / S181D / H183Y / F187V / D203R / F208D / V229I / S248R, S27L / L90Y / T109L / E173W / A174R / S181D / H183Y / D203S / F208D / A215D, A24V / S27L / W69L / L90Y / D203N / F208Q / A215D / V229I / S248R, P20T / S27L / W69I / L90Y / E173T / A174K / T176S / F187V / N204G / F208V / A215D / V229I, P20T / S27L / W69S / L90Y / F187V / S212Q / P213D / I222L / V229I, V23T / A24V / S27L / W69H / L90Y / T109L / S110R / T176S / P213D / I222L / V229I / S248R, R12T / V23T / S27L / L90Y / S212Q / S248R, S27L / L90Y / P213D, S27L / W69G / L90Y / A174R / S212Q / P213D, V23T / S27L / L90Y / D203A / N204G / A215D / I222L / V229I / S248R, S27L / L90Y / A174K / T176S / F187V / D203G / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / E173N / A174K / D203S / F208Q, S27L / W69I / L90Y / T157L / E173N / A215D, A24V / S27L / L90Y / S110R / S181D / H183S / D203S / N204G / A215D, R12T / P20T / S27L / L90Y / S212Q / P213D, R12T / P20T / S27L / L90Y / T176S / V177T / I222L / V229I / S248R, S27L / L90Y / T109L / H183S / D203K / F208D / V229I / S248R, R12T / S27L / L90Y / T109L / A174R / S181D / F208K / I222L / S248R, S27L / W69C / L90Y / I222L / V229I / S248R, P20T / S27L / L90Y / A174K / T176S / H183S / D203K / N204G, S27L / W69C / L90Y / T176S / S212Q / P213D / V229I / S248R, A24V / S27L / W69G / L90Y / S110R / D203G / N204G / A215D / I222L / V229I,S27L / L90Y / A174R / T176S / D203G / F208Q / A215D / S218R / I222L / V229I / S248R, S27L / L90Y / S110R / E173N / F187V / F208Q / A215D / I222L / V229I / S248R, P20T / S27L / L90Y / V177T / F187V / D203S / A215D / I222L / S223N / V229I, S27L / L90Y / T109L / S181D / H183S / D203R / N204G / A215D / I222L / V229I / S248R, V23T / A24V / S27L / L90Y / E173N / A174R / D203A / A215D / I222L, P20T / S27L / W69L / L90Y / F187V / F208Q / S248R, P20T / S27L / W69H / L90Y / E173T / A174K / S181D / A215D, V23T / S27L / L90Y / E173V / A174R / S181D / F187V / D203R / A215D / I222L / V229I, S27L / W69H / L90Y / T109L / S181D / D203S / N204G / A215D, S27L / L90Y / N204G / F208D / I222L, S27L / L90Y / T109L / S110R / S181D / H183A / D203G / N204G / F208K / I222L, 12T / A24V / S27L / L90Y / E173P / A174K / A215D, A24V / S27L / W69H / L90Y / T109L / S110R / A174K / T176S / D203S / S212Q / P213D / I222L / V229I, A24V / S27L / L90Y / S212Q / P213D / I222L / V229I, R12T / P20T / S27L / L90Y / T176S / V177T / P213D / I222L / V229I, S27L / L90Y / E173P / S181D / D203K / N204G / S212Q / P213D / I222L / V229I, S27L / W69I / L90Y / S110R / H183S / D203R / N204G / A215D, A24V / S27L / W69C / L90Y / T109L / S110R / T176S / D203A / A215D / I222L / S248R, V23T / S27L / L90Y / H183Y / A215D / S248R, S27L / L90Y / T176S / D203S / P213D, S27L / L90Y / S181D / H183A / P213D / V229I, S27L / L90Y / I222L / V229I / S248R, R12T / S27L / L90Y / A174K / P213D / I222L / V229I, R12T / T16A / V23T / A24V / S27L / L90Y / T176S / V177T / A215D / I222L / V229I / S248R, S27L / W69H / L90Y / T109L / D203S / N204G / P213D, V23T / A24V / S27L / L90Y / V177T / D203S / N204G / A215D / I222L / V229I, S27L / L90Y / A174K / T176S / F208Q / A215D / I222L / S248R, A24V / S27L / L90Y / S181D / H183T / D203K / N204G / F208Q / A215D / I222L / V229I, A24V / S27L / L90Y / V177T / D203K / A215D / V229I / S248R, V23T / A24V / S27L / L90Y / D203R / N204G / A215D / S248R, R12T / S27L / L90Y / T109L / S181D / H183T / N204G / F208Q / S212Q / P213D / I222L / V229I / S248R, A24V / S27L / L90Y / F208Q / I222L / V229I / S248R, S27L / L90Y / A174R / T176S / H183T / P213D / V229I, V23T / A24V / S27L / L90Y / E173W / A174K / D203S / F208D, A24V / S27L / W69L / L90Y / S181D / H183Y / F187V / F208V / A215D / S248R, R12T / S27L / L90Y / E173W / A174K, V23T / A24V / S27L / L90Y / D203G / N204G / A215D / I222L / V229I, S27L / L90Y / D203A / N204G / F208V / A215D, V23T / S27L / L90Y / T109L / S110R / A174K / T176S / N204G / F208Q / I222L / V229I, S27L / L90Y / E173P / A174K / D203G / F208V / I222L / V229I, A24V / S27L / W69H / L90Y / S110R / D203R / N204G / F208K,P20T / S27L / L90Y / E173N / A174K / H183Y / D203K / F208Q / I222L / V229I, P8L / A24V / S27L / L90Y / T109L / S110R / A215D / I222L / V229I, P20T / S27L / L90Y / S110R / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / S110R / E173N / A174K / S181D / F187V / F208Q / I222L / V229I, A24V / S27L / W69L / L90Y / P213D, S27L / L90Y / S218G / I222L / V229I / S248R, A24V / S27L / L90Y / E173T / A174R / F208V / V229I, S27L / W69I / L90Y / S181D / F187V / A215D / I222L / V229I / S248R, S27L / W69G / L90Y / E173P / A174R / S212Q / V229I, P20T / S27L / L90Y / E173W / A174R / S181D / F187V / D203R / N204G / P213D / I222L / V229I, S27L / L90Y / A174R / T176S / H183D / F187V / D203R / N204G / F208V / A215D / V229I, R12T / P20T / S27L / L90Y / E173P / S181D / D203K / N204G / F208K, P20T / S27L / L90Y / T109L / D203G / I222L / V229I / S248R, S27L / L90Y / E173N / A174K / F208K, R12T / V23T / S27L / L90Y / E173V / A174R / F208D / S212Q / I222L / V229I, V23T / A24V / S27L / L90Y / S110R / E173N / A174K / H183T / D203R / N204G / F208Q / I222L / V229I, S27L / W69L / L90Y / T109L / E173V / A174K / D203G / A215D / I222L / V229I, A24V / S27L / L90Y / T176S / V177T / H183Y / D203K / S212Q / P213D / I222L / V229I, P20T / S27L / L90Y / V177T / A215D / I222L / S248R, V23T / A24V / S27L / L90Y / P213D / V229I, S27L / L90Y / D203K / N204G / V229I, A24V / S27L / W69I / L90Y / E173W / S181D / A215D, S27L / W69S / L90Y / A174R / D203S / N204G / F208Q / A215D / V229I / S248R, S27L / L90Y / T176S / A215D / I222L / V229I / S248R, V23T / S27L / L90Y / E173N / A174K / I222L / V229I, S27L / L90Y / A215D / V229I / S248R, P20T / S27L / L90Y / A174R / T176S / N204G / F208Q, S27L / L90Y / E173V / A174K / F187V / D203S / F208Q / I222L / V229I, V23T / S27L / L90Y / V177T / F187V / S212Q / P213D, S27L / L90Y / A174R / S181D / F208V / A215D / I222L / V229I / S248R, P20T / S27L / L90Y / S181D / H183A / S212Q / I222L / V229I / S248R, S27L / L90Y / D203G / N204G, V23T / S27L / L90Y / P112Q / E173T / A174K / H183Y / D203R / F208D / A215D / V229I / S248R, R12T / S27L / L90Y / H183Y / S212Q / I222L / V229I / S248R, S27L / W69S / L90Y / H183Y / A215D / I222L / V229I / S248R, S27L / L90Y / H183Y / A215D / S248R, V23T / S27L / L90Y / T109L / S110R / D203K / N204G / F208D / I222L / V229I, R12T / A24V / S27L / L90Y / S110R / S181D / H183Y / P213D, S27L / L90Y / S248R, R12T / P20T / S27L / W69H / L90Y / T109L / S110R / P213D / V229I, S27L / W69G / L90Y / T109L / E173T / A174K / A215D / V229I, A24V / S27L / L90Y / A174R / P213D, V23T / A24V / S27L / L90Y / A174K / T176S / P213D / V229I, A24V / S27L / L90Y / A174K / A215D / I222L / V229I / S248R, R12T / S27L / W69G / L90Y / E173N / A174K / D203K,V23T / A24V / S27L / L90Y / E173W / A174K, P20T / S27L / L90Y / D203S / N204G / A215D / I222L / V229I, R12T / S27L / L90Y / A215D / S248R, R12T / P20T / S27L / W69C / L90Y / T109L / E173V / A174R / D203A, A24V / S27L / W69C / L90Y / V177T / F208K / S212Q / P213D / S248R, S27L / L90Y / T109L / S110R / E173V / A174K / A215D, R12T / P20T / S27L / L90Y / E173T / A174K / F187V / D203A / N204G / A215D / I222L / V229I, S27L / L90Y / E173N / S181D / F187V / F208K / S248R, R12T / S27L / L90Y / T176S / V177T / A215D / S248R, A24V / S27L / L90Y / S110R / E173P / A174R / H183Y / A215D / S248R, R12T / P20T / S27L / L90Y / D203S / N204G / F208V / A215D, S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / P213D / V229I, S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / S181D / H183S / D203S / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D / V229I, S27L / W69I / L90Y / T176S / S181D / H183S / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / S181D / H183Y / D203A / F208V / P213D / A215D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / D203A / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183S / D203S / F208V / P213D, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D, R12T / S27L / L90Y / S181D / H183Y / D203A / F208V / P213D / V229I, R12T / S27L / L90Y / T176S / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D / A215D, S27L / W69I / L90Y / S181D / H183T / D203A / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / S181D / H183S / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / H183Y / F208V / P213D / A215D, R12T / S27L / L90Y / V177T / S181D / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / P213D / A215D, S27L / W69I / L90Y / S181D / H183Y / D203A / F208Q / S212Q / P213D, S27L / W69I / L90Y / T176S / V177T / S181D / H183S / F208V / P213D,R12T / S27L / W69I / L90Y / S181D / H183Y / F208Q / P213D / V229I, R12T / S27L / L90Y / S181D / H183Y / D203S / F208V / P213D, R12T / S27L / W69I / L90Y / S181D / D203A / F208V / P213D / V229I, S27L / W69I / L90Y / S181D / H183S / F208V / S212Q / P213D / V229I, S27L / W69I / L90Y / V177T / S181D / H183Y / F208V / S212Q / P213D, R12T / S27L / W69I / L90Y / V177T / S181D / H183S / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / D203A / F208V / P213D / V229I, R12T / S27L / W69I / L90Y / V177T / H183Y / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183S / F208V / P213D / A215D, S27L / W69I / L90Y / T176S / S181D / H183Y / F208Q / P213D / A215D, R12T / S27L / W69I / L90Y / V177T / S181D / D203A / F208V / P213D, S27L / W69I / L90Y / V177T / S181D / H183S / F208V / S212Q / P213D, and R12T / S27L / W69I / L90Y / S181D / H183A / D203A / F208Q / P213D.

19. A composiƟon comprising a variant Bhr-PETase as compared to SEQ ID NO:5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity.

20. The composiƟon according to claim 19, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 20, 23, 24, 69, 109, 110, 157, 174, 176, 177, 181, 183, 187, 203, 204, 208, 212, 213, 215, 216, 222, 229 and 246, wherein said variant Bhr-PETase has at least 85% idenƟty to SEQ ID NO: 5 and has PETase acƟvity.

21. The composiƟon according to any one of claims 19 or 20, wherein said composiƟon comprises a variant Bhr-PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246.

22. The composiƟon according to any one of claims 19 to 21, wherein said composiƟon comprises a variant Bhr-PETase as compared to SEQ ID NO: 5, wherein said variant comprises at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 12, 176, 177, 181, 204, 212, 213 and 215.

23. The composiƟon according to any one of claims 19 to 22, wherein said composiƟon comprises a variant Bhr-PETase as compared to SEQ ID NO: 5, wherein said variant comprises at leastone amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 90, 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249.

24. The composiƟon according to claim 19 to 23, wherein said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 20, 23, 24, 69, 109, 110, 157, 174, 183, 187, 203, 208, 216, 222, 229 and 246.

25. The composiƟon according to claim 19 to 24, wherein said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 176, 177, 181, 204, 212, 213 and 215.

26. The composiƟon according to any one of claims 19 to 25, wherein said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 20, 23, 24, 69, 109, 110, 157, 174, 176, 177, 181, 183, 187, 203, 204, 208, 212, 213, 215, 216, 222, 229 and 246.

27. The composiƟon according to any one of claims 19 to 26, wherein said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 14, 87, 89, 91, 92, 156, 170, 173, 182, 206, 209, 211, 214, 217, 248 and 249.

28. The composiƟon according to any one of claims 19 to 27, wherein said variant comprises a subsƟtuƟon at posiƟon 90, and at least one amino acid subsƟtuƟon compared to SEQ ID NO: 5 at an amino acid posiƟon(s) selected from the group consisƟng of 12, 14, 20, 23, 24, 69, 87, 89, 91, 92, 109, 110, 156, 157, 170, 173, 174, 176, 177, 181, 182, 183, 187, 203, 204, 206, 208, 209, 211, 212, 213, 214, 215, 216, 217, 222, 229, 246, 248 and 249.

29. The composiƟon according to any one of claims 19 to 28, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, P20T, V23T, A24V, W69H, W69I, W69L, W69S, T109L, S110R, T157A, T157L, A174R, H183S, H183T, F187I, F187L, D203K, D203R, F208D, F208G, F208S, F208T, A216D, A216E, A216T, I222L, V229I, and A246Q.

30. The composiƟon according to any one of claims 19 to 29, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of W69F, H183Y, D203A, D203S, F208Q, and F208V.

31. The composiƟon according to any one of claims 19 to 30, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of R12T, T176S, V177T, S181D, N204G, S212Q, P213D, and A215D.

32. The composiƟon according to any one of claims 19 to 31, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, T109L, S110R, T157A, T157L, A174R, T176S, V177T, S181D, H183S, H183T,H183Y, F187I, F187L, D203A, D203K, D203R, D203S, N204G, F208D, F208G, F208Q, F208S, F208T, F208V, S212Q, P213D, A215D, A216D, A216E, A216T, I222L, V229I, and A246Q.

33. The composiƟon according to any one of claims 19 to 32, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, T157D, T157E, T157G, T157Q, T157S, A174G, D203E, D203G, D203L, D203N, D203V, D203Y, A216I, A246E, and A246N.

34. The composiƟon according to any one of claims 19 to 33, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, V177I, V177W, S181A, S181Q, S181T, N204S, S212E, S212H, S212I, P213A, A215K, and A215S.

35. The composiƟon according to any one of claims 19 to 34, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, R12E, R12F, R12L, R12M, R12Q, R12S, T157D, T157E, T157G, T157Q, T157S, A174G, V177I, V177W, S181A, S181Q, S181T, D203E, D203G, D203L, D203N, D203V, D203Y, N204S, S212E, S212H, S212I, P213A, A215K, A215S, A216I, A246E and A246N.

36. The composiƟon according to any one of claims 19 to 35, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of A14V, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, H156N, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, Q182D, Q182E, Q182S, T206D, T206S, A209E, N211Q, N214D, I217M, S248C, S248R, and D249L.

37. The composiƟon according to any one of claims 19 to 36, wherein said amino acid subsƟtuƟon is selected from the group consisƟng of L90A, L90F, L90Y, R12E, R12F, R12L, R12M, R12Q, R12S, R12T, A14V, P20T, V23T, A24V, W69F, W69H, W69I, W69L, W69S, N87K, R89I, R89K, R89M, D91N, F92D, F92G, F92Q, T109L, S110R, H156N, T157A, T157D, T157E, T157G, T157L, T157Q, T157S, V170I, V170L, E173A, E173N, E173P, E173R, E173T, E173V, E173W, A174G, A174R, T176S, V177I, V177T, V177W, S181A, S181D, S181Q, S181T, Q182D, Q182E, Q182S, H183S, H183T, H183Y, F187I, F187L, D203A, D203E, D203G, D203K, D203L, D203N, D203R, D203S, D203V, D203Y, N204G, N204S, T206D, T206S, F208D, F208G, F208Q, F208S, F208T, F208V, A209E, N211Q, S212E, S212H, S212I, S212Q, P213A, P213D, N214D, A215D, A215K, A215S, A216D, A216E, A216I, A216T, I217M, I222L, V229I, A246E, A246N, A246Q, S248C, S248R, and D249L.

38. The composiƟon according to any one of claims 19 to 37, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, P20T, V23T, A24V, W69H, A174R, H183T, F208D, I222L, and V229I.

39. The composiƟon according to any one of claims 19 to 38, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, W69F, D203A and D203S.

40. The composiƟon according to any one of claims 19 to 39, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, T176S, V177T, and S181D.

41. The composiƟon according to any one of claims 19 to 40, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, A174R, T176S, V177T, S181D, H183T, D203A, D203S, F208D, I222L, and V229I.

42. The composiƟon according to any one of claims 19 to 41, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of W69I, W69L, H183S, H183Y, D203G, D203R, F208Q, and F208V.

43. The composiƟon according to any one of claims 19 to 42, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of N204G, S212Q, P213D, and A215D.

44. The composiƟon according to any one of claims 19 to 43, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons of L90Y, R12T, P20T, V23T, A24V, W69F, W69H, W69I, W69L, A174R, T176S, V177T, S181D, H183S, H183T, H183Y, D203A, D203G, D203R, D203S, N204G, F208D, F208Q, F208V, S212Q, P213D, A215D, I222L, and V229I.

45. The composiƟon according to any one of claims 19 to 44, wherein said variant Bhr-PETase enzyme has one or more amino acid subsƟtuƟons at one of said posiƟons, two of said posiƟons, three of said posiƟons, four of said posiƟons,five of said posiƟons, six of said posiƟons, seven of said posiƟons, eight of said posiƟons, nine of said posiƟons, ten of said posiƟons, eleven of said posiƟons, twelve of said posiƟons, thirteen of said posiƟons, fourteen of said posiƟons,fiŌeen of said posiƟons, sixteen of said posiƟons, seventeen of said posiƟons, eighteen of said posiƟons, nineteen of said posiƟons or twenty of said posiƟons.

46. The composiƟon according to any one of claims 19 to 45, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y, P20T / V23T / A24V / W69L / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69S / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69L / T109L / S110R / A174R / H183S / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69L / L90Y / T109L / S110R / H183S / F187I / D203K / F208T / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / A174R / H183S / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / H183S / F187L / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A246Q, P20T / V23T / A24V / W69I / L90Y / T109L / S110R / T157L / H183T / F187L / D203R / F208S / A216D / I222L / V229I / A246Q,P20T / V23T / A24V / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216E / I222L / V229I / A246Q, P20T / V23T / A24V / W69L / T109L / S110R / A174R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69I / T109L / S110R / H183T / F187L / D203R / F208S / A216T / I222L / V229I / A246Q, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203K / F208G / I222L / V229I, P20T / V23T / A24V / W69H / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / H183S / F187I / D203R / F208G / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / A174R / H183S / D203R / F208D / I222L / V229I, P20T / V23T / A24V / W69H / L90Y / T109L / S110R / T157A / A174R / H183S / D203R / F208D / I222L / V229I, W69H / H183S / F187I / D203K / F208G, W69H / L90Y / H183S / F187I / D203K / F208G, W69H / H183S / F187I / D203R / F208G, W69H / L90Y / H183S / F187I / D203R / F208G, W69H / L90Y / A174R / H183S / D203R / F208D, and W69H / L90Y / T157A / A174R / H183S / D203R / F208D.

47. The composiƟon according to any one of claims 19 to 46, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12T / L90Y / A174R / T176S / H183Y / F208Q / I222L / V229I, R12T / P20T / W69L / L90Y / S181D / D203R / F208V / I222L / V229I, R12T / L90Y / A174R / T176S / H183T / F208D / I222L / V229I, R12T / W69H / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / W69H / L90Y / S181D / D203A / F208D / I222L / V229I, R12T / W69F / L90Y / T176S / V177T / S181D / H183T / F208D, R12T / W69F / L90Y / S181D / D203A / F208D / I222L / V229I, V23T / A24V / L90Y / N204G / F208V / S212Q / P213D / I222L / V229I, R12T / P20T / L90Y / D203S / N204G / F208V / A215D, R12T / W69I / L90Y / V177T / S181D / H183S / F208V / P213D, R12T / W69I / L90Y / S181D / H183Y / F208V / P213D / A215D, R12T / W69I / L90Y / T176S / S181D / H183Y / F208V / P213D, R12T / W69I / L90Y / V177T / H183Y / D203A / F208V / P213D, and R12T / W69I / L90Y / V177T / S181D / D203A / F208V / P213D.

48. The composiƟon according to any one of claims 19 to 47, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / T176S.

49. The composiƟon according to any one of claims 19 to 48, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of D203A, S181D, D203R, R12T, V177T, L90Y / T176S, T176S, A215D, N204G, and D203S.

50. The composiƟon according to any one of claims 19 to 49, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of L90Y / A246E, A174G / D203A / A216E, and L90Y / A174G.

51. The composiƟon according to any one of claims 19 to 50, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12F / L90Y, R12T / L90A / T176S / S212E, and R12Q / T176S.

52. The composiƟon according to any one of claims 19 to 51, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, T176S / V177T / A215D / A246E, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, and T176S / D203A / A216I.

53. The composiƟon according to any one of claims 19 to 52, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of A215D / A246E, R12E / A215D / A216T / A246E, R12T / A174G / T176S, T157D / T176S / V177T, R12F / T157E / T176S / V177I / S212E / A215D / A216T / A246Q, R12F / L90Y, R12T / T157Q, V177I / A215D / A216T, L90F / A215D / A216T, A174G / T176S / A246E, T157S / S212Q, L90F / A215D / A246E, V177I / A216T, R12E / D203R, R12E / T157L / A215D, R12E / T176S / D203R / A246E, R12E / V177I / D203R, R12E / T176S / V177T / D203R / A215D, A174G / T176S / D203G / A215D, L90Y / A246E, A174G / D203A / A216E, L90Y / T176S / V177I / D203E, R12E / L90Y / T157E / D203K / A246E, R12Q / T176S, R12Q / T157L / T176S / V177I, L90Y / A174G / T176S / A216E, L90Y / A174G, T176S / D203A / A216I, R12T / L90A / T176S / S212E, and T176S / V177T / A215D / A246E.

54. The composiƟon according to any one of claims 19 to 53, wherein said variant Bhr-PETase comprises a set of amino acid subsƟtuƟons selected from the group consisƟng of R12E / V170I / V177T / S212H, R12F / V170L / T176S / V177I / S181D, R12F / T157L / V170I / T176S / D203A / S212E, L90F / V170I / T176S / V177I / Q182D / A216T, L90Y / T157Q / V170I / S181Q / Q182D, R12T / L90F / F92G / V170I / A215D, R12T / V170L / S181T / Q182D, L90Y / A174G / T176S / V177I / Q182E, L90Y / V170I / T176S / V177I / Q182D / A246E, R12E / R89K / L90F / V170I / T176S, R12M / V170I / T176S / V177I / S212Q, R12M / V170L / A174G / S181D, R12L / F92G / T157E / V170L / S181T / Q182D / T206D / S212Q / A246Q, V170I / S181Q / Q182E / D203Y / A215D, R12M / R89K / L90Y / V170L / T176S / V177I / T206D / S212H, R12L / R89K / L90Y / V170I / V177I / S181Q / Q182D, R12S / L90Y / A246N / S248R, R12S / Q182E / D203S / A216T, R12T / V170I / D203A / S212H, T157Q / V170L / T176S / V177T / A215D / A216T, R12E / E173A / V177T / A215D / A216T, F92G / T157E / A174G / T176S / V177W / S181D / A215D / A216T, E173T / S181D / D203N,R12S / V170L / A174G / S181D, E173P / S181T / D203R / A216T / A246E / S248C, R12T / A174G / S181T / Q182D / A246E / S248R, R12S / A215D / A216T / S248C, R12M / T157S / V170L / T176S / V177T / S181D / Q182D, R12T / Q182D / A215D / A216T, V170L / A174G / T176S / Q182D / S212Q / A246E, R12S / V170L / A215D / A216T / A246E, R12T / L90A / E173P / A174G / S181D / Q182E, R89M / T157G / S181T / Q182E, R89K / V170I / T176S / V177I / D203S, L90F / F92D / V170L / T176S / D203S / T206D / S212H, V170I / E173T / S181T / Q182D / D203G / A215D / A246E, V170L / T176S / Q182D, T157Q / V170I / V177T / T206D / S212Q / D249L, R12E / T157E / V170I / A174G / T176S / Q182D, R12F / T157Q / V170I / D203G / S212Q / D249L, R89K / L90F / V170I / V177T, R12T / E173A / A174G / S181T / Q182D, T157A / V170L / V177T, E173P / S181D, E173R / A174G / A209E / A215D / A246Q, L90F / V170I / Q182E / A215D, F92G / T157L / V170I / T176S / S181Q / Q182E / S212H, R12T / E173T / A174G / S181D / Q182D, R12T / R89K / L90Y / T157A / V170I / A174G / T176S, L90Y / V170I / V177I / A215D / A216T, V170I / V177I / T206D / A215D, L90F / T157E / V170I / T176S / V177I / S212H, R12T / V170I / T176S / V177T, V170I / T206D / S212Q, R12E / L90F / V170I / T176S / S181T / Q182D, V170I / T176S / V177T / A246E, V170I / T176S / D203K, L90F / F92G / V170I / S181Q / Q182D / T206D / A215D, T157A / D203V / A209E / A215D, T157E / V170L / V177I / Q182E / A215D / A246N, T157Q / V170I / S181D / S212H, V170I / T176S / V177I / Q182D / N204G / T206D, T157G / V170I / S181T / S212Q, L90Y / E173R / Q182D / N204G / T206D / S212H, R89K / L90F / T176S / V177I, T157S / E173T / A174G / S181T / Q182E / A215D / A246N, L90F / F92G / V170I / T176S / V177T, E173N / S181D / S212E, R12S / E173P / S181D, R12E / T157L / E173N / A174G / S181D / Q182D, R12T / V170I, R12T / T157Q / V170I / A174G / T176S / A215D / A216T, L90Y / E173N / S181T / Q182E, L90F / V170L / T176S / V177T / A215D, R12T / R89K / L90F / V170I / S181D / Q182D / D249L, R12T / L90A / E173N / A209E, V170I / A215D / A216T, V170L / V177T, V170L / V177T / T206D / A215D, V170I / E173T / S181D, L90A / T157S / V170L / V177I / T206D / S212Q, V170L / T176S / V177I / S181D / D249L, T157A / A174G / T176S / S181D / Q182D / A215D / A216T, L90F / F92G / T157Q / V170I / V177W / A209E / A215D / A216T, V170I / A174G / T176S / Q182E / T206D / A216T, L90Y / F92G / T157G / V170I / T176S / V177I / D203A / S212Q / A246E, T157G / A209E / A215D / A246Q, R89K / L90Y / T157S / A174G / S181T / Q182D, V170I / V177T, V170L / T176S / A215D, E173N / S181D / D203G / A209E / A215D / A216T, T157Q / V170I / V177T / A246E, R12T / L90F / E173R / S181Q / Q182E / A209E, F92G / T157Q / E173R / A174G / S181D / Q182D / A215D / A216T / S248R / D249L, R12T / R89K / L90Y / V170I, T157G / V170I / Q182E / D203V, F92G / T157Q / V170I, T206D, E173T / A174G / Q182D / D203G / A216T, L90Y / T157S / V170L, R12T / T157L / V170L / V177T / D203S / A215D,R12E / V170I / A174G / Q182D / D203R / A215D / A216T, R12S / R89K / T157L / V170I / T176S / S181D / Q182D / D203G / A216T, R12S / R89K / L90Y / T176S / V177I / Q182D / D203A, R12T / R89K / V170I / V177T, R12E / E173P / S181T / Q182D / A215D / A216T, R12S / L90Y / S181T / Q182D / D203R, R12E / L90Y / T157L / E173A / A215D / A216T / A246E, R12E / V170L / D203R / A215D / A246E, R12E / L90Y / V170I / E173T / Q182D / A215D / A246E, R12T / L90Y / V170L / A215D, R12E / V170I / V177T / D203R, R12E / V170L / T176S / A215D, R12E / F92G / V170I / A174G / T176S / D203R / A216T, R12E / V170I / E173P / S181T / D203R, R12E / L90Y / V170I / A174G / V177I / S181D / A215D / A216T / A246E / S248R, R12E / V170L / D203R / A246E, V170L / V177T / A246E, R12T / L90Y / V170I / D203G, R89K / L90Y / V177I / S181D / D203G / A215D / A216T, R12T / T157S / E173P / A174G / S181D / D203R / A246E / S248R, R12E / L90Y / V170L / A174G / S181T / Q182D, R12E / S181T / Q182D / D203R / A215D, R12E / L90F / F92G / E173A / S181D / D203R, R12S / V170L / T176S / S181D, R12S / V170I / V177T / D203R, R12T / L90Y / V170L / D203A, L90Y / V170I / T176S / V177T / S181D / D203R / A246E / S248R, R12S / V170I / T176S / V177T / S181D / Q182D / A246E, R12S / V170I / Q182D / A215D / A216T, R12T / L90Y / V170I / V177T, L90Y / E173P / A174G / S181D / D203G, R12S / R89K / L90A / V170I / A174G / T176S / S181D / Q182D, R12T / L90F / E173A / A174G / S181D / A216T / D249L, L90Y / V170I / T176S / V177I / S181D / Q182D / A246E / S248C, R12T / R89K / L90Y / T157A, L90Y / H156N / T157L / T176S / N204G / T206S / A215D, V170I / T176S / V177T / D203R / A246E / S248R, R12E / V170L / S181D / A215D, R12T / T157E / V170L / T176S / S181T / D203R / A215D / A246E, R12T / L90Y / V170L / V177I / A216T, R12E / L90Y / T157A / E173A / S181T / Q182D / A215D / A216T, L90Y / V170I / V177T / A216T, R12E / N87K / R89K / V170I / T176S / V177I / D203R / A215D / A216T / D249L, R12E / V170I / T176S / V177I / S181D / Q182D, L90Y / F92G / T157A / V170I / D203G / A216T / A246E / S248C, R12T / L90Y / V170L / T176S / V177T / D203R / A216T, R12T / L90F / V170I / S181D, R12T / L90Y / V170L / T176S / V177T / D203R / A246E, F92G / V170I / V177I / S181D, R12E / F92G / V170I / T176S / V177T / A215D / A216T / A246E, R12T / V170I / T176S / V177T / D203R, R12E / L90Y / E173P / A174G / S181T / Q182D, R12E / L90Y / V170I / T176S / S181T / D203R / A216T / A246E, R12T / D203R / D249L, R12E / L90Y / T157L / V170L / T176S / V177T / S181D / Q182D / D203R / A246E, R12S / V170L / T176S / V177I, L90Y / V170L / T176S / V177I, R12E / L90F / F92G / V170L / V177T / D203R / A216T, L90Y / V170L / V177T / D203G / A216T / A246E, V170I / A174G / S181D / D203R / A246E / S248R, R12E / T157E / T176S / Q182D / A215D / A216T, S181A / Q182S / D203S, R12S / V170I / S181D / D203R / A215D / A216T, R12E / V170L / T176S / S181D / Q182D / A216T, L90F / F92G / V170I / V177T / D203R / A216T, R12S / E173P / S181T / D203R / A215D / A216T / A246E,R12E / R89K / L90Y / D203R, R12E / F92G / A215D / A216T / A246E / S248R, R12S / T157S / E173T / A174G / D203R / A215D, R12E / L90A / E173P / A174G / S181T / Q182D / A215D / A216T, R89I / L90Y / H156N / T157L / V177I, L90Y / D249L, R89I / A174G / T176S / A215K, F92G / T157A / E173P / A174G / S181D / A216T, R12E / F92G / T176S / V177I, R12T / V170L / T176S / V177T / D203R / A216T, L90Y / F92Q / N204G / N211Q / A215S, L90Y / F92G / Q182S / N211Q / S212I / A216E / I217M, T176S / N211Q, L90F / D203R / D249L, R12T / L90Y / E173P / A174G / A246E, R89K / V170L / T176S / V177I / A215D, R89I / L90Y / T176S / S181A / Q182S / N204S / P213A / N214D, R12L / R89K / L90Y / V170L / T176S / V177I / N204G / A215D, R89K / V170L / T176S, T157S / V170I / V177T / S181T / Q182D / D203S / S212E / A246Q / S248C, D203G / A216E / I217M, R12T / R89K / T157G / D203N / S212E / A246E, E173R / A174G / Q182D / D203R / A215D / A216T / A246N / S248C, R12S / L90Y / V170I / E173A / A174G / S181T / Q182D / S248C / D249L, R12T / R89K / L90A / E173R / A174G / S181T / Q182D / A215D / A216T, R12T / V177T / D203V / A215D / A246N / S248C, R12T / V170L / V177T / S181T / A216T, R12T / R89K / L90Y / Q182D / A246E / S248C, R12E / F92G / V170L / V177T / A215D / S248C, R12T / L90A / D203Y / A209E / S212Q / A246Q, R12M / E173P / A174G / S181D / A209E / S212Q / A246E / S248R, R12T / R89K / L90Y / S212H / A215D / A246Q / S248C, R12T / R89K / L90A / A174G / T176S / S181T / Q182D / A215D / A246Q, R89K / L90F / A174G / S181D / S212E / A246E / S248C, R12M / E173R / S181T / Q182E / S212Q / A216T, R12L / R89K / L90Y / T157D / E173P / A174G / S181Q / Q182D / S212H, R12T / R89K / L90A / A215D / A216T / D249L, R12T / L90A / A246E / S248C, E173R / Q182D / A215D / A216T / S248R / D249L, R89K / L90Y / T176S / V177W / D203Y / A216T, R12M / V170I / A174G / T176S / S181T / T206D / A246Q / S248C, R12F / V170L / V177I / S181D / Q182E / S212E / A246E, R12T / R89K / L90F / V170L / T176S / S181Q / A216T, R12E / L90A / E173R / S181Q / Q182E / A209E / S212Q / A246N / S248C, R12T / T157Q / V170L / T176S / V177T / T206D / A209E / A216T, R12T / R89K, R12T / T157Q / V170I / E173N / S181T / Q182D / A215D / A216T / A246Q / S248C, R89K / L90Y / T206D / A209E / S212H / S248C, R12T / R89K / L90Y / A216T, R89K / T176S / V177I / S181Q / T206D / A209E / A215D / A216T / A246E, V170L / E173N / V177T / Q182D / T206D / A216T / A246Q, R12T / Q182E / D203V / A216T / A246E / S248C, L90Y / V170I / V177T / D203Y / S212E / A246E / S248C, R89K / L90Y / V170L / A174G / T176S / S181Q / S212E / A215D / A216T / A246E, T157Q / V170L / T176S / Q182D / A209E / A216T / A246Q, R12E / T157Q / E173R / A174G / Q182E / D203L / N204G / S212Q / A246E / S248C,R12M / A14V / V170I / V177T / S212H / A246E / S248C, R12L / L90A / D91N / F92G / A215D / A216T, R12T / V170L / V177T / A216T, L90A / S212Q / A246E / S248C, R12L / R89K / L90Y / V170I / V177I / D203R, R12T / S181D / Q182D / A216T / A246E, R89K / L90Y / E173T / S181T / N204G / T206D / A215D / A246N / S248C, V170L / S181D / Q182E / S212H / A246E, V170I / E173P / S181D / Q182E / A216T, R12L / V170L / T206D / A209E / A215D / A216T / D249L, T157D / E173P / A174G / Q182E / T206D / A215D / A216T, T176S / V177W / A246N / S248C, R89K / L90A / E173W / A215D / A216T / A246E / S248C, L90Y / T157G / A174G / T176S / A216T / A246Q / S248C, R12S / F92G / V170L / T176S / A209E / A215D / A216T, R12T / S248C / D249L, R12L / V170L / V177T / D203K / S212H / S248C / D249L, R12T / V170L / T176S / V177T / A209E, R12T / L90Y / F92G / V170I / V177I / S212E / A246Q / S248C, R12E / R89K / L90A / V170I / V177I / T206D / A215D / A216T, A174G / D203G / A216T / S248C / D249L, T157S / E173A / S181D / S248R / D249L, L90Y / E173V / S181D / T206D / S212Q / A246Q, T157Q / V170I / T176S / T206D / A215D / A246N / S248C, F92G / T176S / A216T, V170L / T176S / V177I / S181T / Q182D / D203A / S212E, F92G / V177W / A215D / A216T, L90Y / E173A / A174G / S181T / Q182D / A216T, F92G / V170I / A174G / T176S / D203N / A215D / A216T / A246E, V170I / T176S / S181Q / Q182E / T206D / S212Q, V170I / T176S / V177T / S181D / D203K / S212E, L90Y / V170I / T176S / N204G / A216T, A174G / T176S / A246N / S248R, T157L / V170I / V177T / S181D / S212E, L90F / F92G / D203S, F92G / V170I / V177T, V170I / T176S / V177W, E173N / T176S / S181T / Q182D / A246E, and V170L / V177I / S181T / Q182D / T206D / A209E / A215D / A216T.

55. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase are more stable than as compared to SEQ ID NOs: 3, 5 or 10.

56. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase are more stable than as compared to SEQ ID NO:

1.

57. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase has improved acƟvity and / or thermostability at a pH above 6.

58. The composiƟon according to any one of the preceding claims, wherein said variant Bhr- PETase has improved acƟvity and / or thermostability at a pH above 6 with respect to SEQ ID NO:

1.

59. A nucleic acid encoding the variant Bhr-PETase enzyme of according to any one of the preceding claims.

60. An expression vector comprising the nucleic acid of claim 59.

61. A host cell comprising the expression vector of claim 60.

62. The host cell according to claim 61, wherein the cell is bacteria, yeast or fungi.

63. A method of making a variant Bhr-PETase enzyme comprising culturing the host cell of claim 61 or 62 under condiƟons wherein said variant Bhr-PETase enzyme is produced, and recovering said variant Bhr-PETase enzyme.

64. A method of degrading PET, comprising contacƟng the PET with the variant Bhr-PETase enzyme of any one of claims 1 to 58.

65. The method according to claim 64, wherein the method degrades the PET in a mixed plasƟcs composiƟon.

66. The method according to claim 64 or 65, wherein the plasƟcs composiƟon comprises analog of PET, PET-like or PET subsƟtute derived biologically or chemically.

67. The method according to any one of claims 64 to 66, wherein the plasƟcs composiƟon comprises at least one selected from the group consisƟng of Polybutylene terephthalate (PBT), Polycabonate (PC), Polycaprolactone (PCL), Polyethylene Furanoate (PEF), and High Density Polyethylene (HDPE).

68. The method according to any one of claims 64 to 67, wherein the contacƟng occurs at least 50°C.

69. The method according to any one of claims 64 to 67, wherein the contacƟng occurs from about 50°C to about 75°C.

70. The method according to any one of claims 64 to 67, wherein the contacƟng occurs from about 55°C to about 65°C.

71. The method according to any one of claims 64 to 67, wherein the contacƟng occurs from about 55°C to about 60°C.

72. The method according to any one of claims 64 to 67, wherein the contacƟng occurs at about 60°C.

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