Flavonoid compound, and preparation method therefor and use thereof

Abstract

The present invention relates to the technical field of natural bacteriostatic active substances, and in particular, to a flavonoid compound, and a preparation method therefor and the use thereof. The preparation method for the flavonoid compound comprises: removing thorns from Echinocactus grusonii, and freeze-drying and grinding the Echinocactus grusonii to obtain Echinocactus grusonii powder; sequentially performing ultrasonic ethanol extraction and organic membrane filtration on the Echinocactus grusonii powder to obtain an extract mother liquor; and separating and purifying the mother liquor to obtain the flavonoid compound. The present invention clarifies the structure of bacteriostatic active ingredients in the Echinocactus grusonii extract, and correspondingly provides a preparation method for the flavonoid compound, thereby improving the biological activity and application value of the extract, ensuring the consistency and repeatability of the extract, and in addition, providing an efficient and stable natural active substance for the antibacterial field.

Description

Flavonoid compounds, preparation method and application thereof TECHNICAL FIELD

[0001] The present application relates to the technical field of natural bacteriostatic active substances, in particular to flavonoid compounds, preparation method and application thereof. BACKGROUND

[0002] Echinocactus grusonii is a perennial succulent plant of the genus Echinocactus in the family Cactaceae. Its chemical composition and properties have attracted extensive research and commercial interest. Echinocactus grusonii is rich in various phytochemicals, including polysaccharides, phenolic compounds, pigments, triterpenes, and saponins. These components confer various biological activities to Echinocactus grusonii, such as anti-inflammatory, antioxidant, antibacterial, anticancer, anti-atherosclerotic, anti-diabetic, neuroprotective, and intestinal protective properties. Therefore, Echinocactus grusonii has potential applications in the food, health product, and pharmaceutical industries.

[0003] Currently, the extraction methods of cactus polysaccharides mainly include hot water method, acid method, enzyme method, ultrasonic wave method, microwave-assisted method, and microbial method, etc. Although the active ingredients in Echinocactus grusonii have various biological activities, existing research shows that the extracts obtained by traditional extraction methods generally perform poorly in antibacterial effect, which severely limits their application in the antibacterial field. SUMMARY

[0004] To solve or partially solve the problems in the related art, the present application provides a flavonoid compound, a preparation method and application thereof.

[0005] The present application provides a preparation method of a flavonoid compound, which comprises:

[0006] The spines of Echinocactus grusonii are removed, and then freeze-dried and ground to obtain Echinocactus grusonii powder;

[0007] The Echinocactus grusonii powder is subjected to ultrasonic alcohol extraction and organic membrane filtration in sequence to obtain an extract mother liquor;

[0008] The mother liquor is separated and purified to obtain a flavonoid compound represented by Formula I:

[0009] Further, the temperature of the freeze-drying is -40℃ to -20℃, and the time is 42h to 72h.

[0010] Further, the temperature of the ultrasonic alcohol extraction is 45℃ to 55℃, and the time is 1.5h to 3.0h.

[0011] Further, the extraction liquid of the ultrasonic alcohol extraction is an ethanol solution with a concentration of 80%, and the solid-liquid ratio of the Echinocactus grusonii powder to the ethanol solution is 1g:(25-45)mL.

[0012] Further, the pore size of the organic membrane is 0.45 microns.

[0013] Further, the separation and purification is specifically ：

[0014] The mother liquor is filtered, silica gel is added to the filtrate, and then vacuum concentration is carried out to obtain the stationary phase; the stationary phase is gradient eluted with dichloromethane and methanol as the developing agent, and the elution phase with a gradient of dichloromethane:methanol = 15:1 to dichloromethane:methanol = 5:1 is collected to obtain the crude product; the crude product is vacuum concentrated to obtain the flavonoid compound shown in formula I.

[0015] Further, the temperature of the vacuum concentration is 45-55 DEG C.

[0016] Further, the mass ratio of the mother liquor to silica gel is (10-18):1; and the gradient elution range of the developing agent is dichloromethane:methanol = 100:1 to dichloromethane:methanol = 1:1.

[0017] The application further provides a flavonoid compound, which has a structure as shown in formula I.

[0018] The application further provides an application of the flavonoid compound in antibacterial products, wherein the antibacterial products include one or more of cosmetics, medicines, health products and food additives.

[0019] The flavonoid compound and the preparation method thereof can have the following beneficial effects:

[0020] 1) The structure of the antibacterial effective component in the extract of the golden echinocactus grusonii is the flavonoid compound as shown in formula I, and a preparation method of the flavonoid compound is provided accordingly, thereby improving the biological activity and application value of the extract, ensuring the consistency and repeatability of the extract, and providing a natural active substance with high efficiency and stability for the antibacterial field.

[0021] 2) The preparation method first carries out freeze-drying before ultrasonic extraction of the golden echinocactus grusonii after removing the spines, thereby reducing the viscosity of the extraction system, making the extraction process more uniform, and improving the consistency and repeatability of the extraction; in addition, the freeze-drying process is carried out at low temperature, thereby avoiding the damage of high temperature to the flavonoid compound and ensuring the yield of the flavonoid compound.

[0022] 3) In the preparation method, the extraction liquid of the ultrasonic extraction is an alcohol solution, the purity of the flavonoid compound in the mother liquor is higher, and the difficulty of subsequent purification of the flavonoid compound is reduced.

[0023] It should be understood that the foregoing general description and the following detailed description are only exemplary and explanatory, and cannot limit the application. Attached Figure Description

[0024] The above and other objects, features and advantages of the present invention will become more apparent from the more detailed description of exemplary embodiments of the invention in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same parts.

[0025] Figure 1 is an HPLC liquid chromatogram of the extract mother liquor in Example 1 of the present invention;

[0026] Figure 2 is an HPLC liquid chromatogram of the golden barrel extract product in Example 1 of the present invention;

[0027] Figure 3 is the LC-MS analysis spectrum of the golden barrel extract product in Example 1 of the present invention, with a liquid phase retention time of 23.104 min;

[0028] Figure 4 is an HDMS negative ion mode mass spectrum of the golden barrel extract product in Example 1 of the present invention;

[0029] Figure 5 is an HDMS positive ion mode mass spectrum of the golden barrel extract product in Example 1 of the present invention;

[0030] Figure 6 is the HNMR spectrum of the golden barrel extract product in Example 1 of the present invention;

[0031] Figure 7 is an HPLC liquid chromatogram of the extract mother liquor in Comparative Example 1 of the present invention;

[0032] Figure 8 is a photograph of the antibacterial effect of the golden barrel extract product in Example 1 of the present invention against Staphylococcus epidermidis and Malassezia. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular forms “a,” “the,” and “the” used in this invention and the appended claims are also intended to include the plural forms unless the context clearly indicates otherwise. It should also be understood that the term “and / or” as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0035] It should be understood that, although the terms "first", "second", "third", etc. can be employed in describing various information in the present application, the information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, the first information can also be referred to as the second information, and similarly, the second information can also be referred to as the first information, without departing from the scope of the present application. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specifically limited.

[0036] The present inventors consider that the reason why the existing cactus extract has no obvious bacteriostatic effect mainly lies in that: on the one hand, the effective component in the extract that plays a role in bacteriostasis is not clear; on the other hand, the destruction of the bacteriostatic effective component in the extraction process leads to poor bacteriostatic effect.

[0037] Therefore, the present inventors first obtain an extract by a traditional extraction method, and then analyze the main components of the extract to preliminarily determine that the components are flavonoids and the molecular weight range (not more than 400). Based on the analysis results, the present inventors further consider using a method with relatively mild temperature conditions and relatively short time to prevent the flavonoids from being destroyed during extraction and to improve the yield. Based on this, the present inventors first use ultrasonic extraction method, which helps to improve the extraction efficiency. However, the present inventors have found through research that when the cactus is subjected to ultrasonic extraction, the extraction system is very viscous. The viscous extraction system leads to the following problems in ultrasonic extraction:

[0038] 1) The propagation of ultrasonic waves is hindered, and the contact area between ultrasonic waves and the extract is reduced, thereby reducing the extraction efficiency.

[0039] 2) The flavonoid components are not uniformly distributed in the viscous system, which leads to the extraction effect in some areas being not as good as that in other areas, affecting the consistency and repeatability of the extraction.

[0040] 3) The heat generated during ultrasonic process is difficult to dissipate in time due to the hindering effect of the viscous system, and excessive heat is accumulated in the extraction system, leading to thermal decomposition of the flavonoid components.

[0041] The present inventors have further found that by freeze-drying the cactus before ultrasonic extraction, the problem of excessively viscous extraction system in ultrasonic extraction can be solved. Moreover, the freeze-drying process has a low temperature and does not cause damage to the flavonoid components.

[0042] Therefore, the present application considers using a method combining freeze-drying and ultrasonic extraction to extract the cactus, and separating and purifying the extract to obtain flavonoids with better bacteriostatic effect.

[0043] The application provides a preparation method of flavonoids, which comprises the following steps:

[0044] The peeled Giallo cactus is freeze-dried and ground to obtain Giallo cactus powder;

[0045] The Giallo cactus powder is subjected to ultrasonic alcohol extraction and organic membrane filtration in sequence to obtain an extract mother liquor;

[0046] The mother liquor is subjected to separation and purification to obtain flavonoids shown in formula I:

[0047] In the preparation method, the Giallo cactus is first peeled to facilitate subsequent operations. Then, the peeled Giallo cactus is subjected to freeze-drying. Freeze-drying can remove the water in the cactus and improve the concentration of the extract. Since the treatment is carried out in a low-temperature environment, the flavonoids can be retained to the greatest extent and the loss of the flavonoids in the water removal process is prevented. More importantly, when the freeze-dried Giallo cactus is subjected to ultrasonic extraction, the viscosity of the extraction system is greatly reduced, which is beneficial to improving the efficiency and uniformity of subsequent ultrasonic extraction. In addition, the damage to the flavonoids is reduced and the difficulty of subsequent purification is reduced.

[0048] In this step, the temperature for freeze-drying is preferably-40℃ to-20℃, and the time is 42h to 72h. Most preferably, the temperature for freeze-drying is-30℃, and the time is 48h. Although the pretreatment of increasing the surface area of the cactus such as slicing before freeze-drying is helpful to improving the efficiency of freeze-drying, the water-solubility of the flavonoids is good, and the flavonoids are easily lost with water during slicing. Therefore, in this application, the peeled Giallo cactus is preferably freeze-dried as a whole. The freeze-dried cactus is ground to obtain Giallo cactus powder. In this way, the contact area between the sample and the extraction liquid can be increased, and the efficiency of subsequent ultrasonic extraction can be improved.

[0049] The Giallo cactus powder can be subjected to ultrasonic extraction. For the extraction liquid, an alcohol solution is selected in this embodiment. Compared with water extraction, the purity of the flavonoids in the mother liquor obtained by alcohol extraction is higher. Further, the extraction liquid obtained by ultrasonic alcohol extraction is an ethanol solution with a concentration of 80%, and the solid-liquid ratio of the Giallo cactus powder to the ethanol solution is preferably 1g:(25-45)mL, and most preferably 1g:30ml. The temperature for ultrasonic alcohol extraction is preferably 45℃ to 55℃, and the time is preferably 1.5h to 3.0h. Most preferably, the ultrasonic temperature is 55℃, and the time is 2.5h. The conditions are beneficial to improving the yield of the mother liquor.

[0050] The mixture after ultrasonic extraction is filtered using an organic membrane to remove solid particles, as well as proteins, polysaccharides and other high molecular substances, so as to improve the purity of the flavonoids. The pore size of the organic membrane is preferably 0.45 μm, which is beneficial to retaining the flavonoids to the maximum extent while filtering out impurities and interfering compounds. The mother liquor containing the flavonoids is obtained after the organic membrane filtration.

[0051] The main components of the mother liquor are characterized, and it is determined that the main components are flavonoids of the structure of Formula I.

[0052] The specific characterization method can be:

[0053] The mother liquor is subjected to HPLC analysis, and it is determined that the main chromatographic peaks are near 23.1 min.

[0054] The mother liquor is subjected to LC-MS analysis, and it is further determined that the molecular weight of the compound corresponding to the chromatographic peak at 23.1 min should be about 300.3.

[0055] The mother liquor is subjected to HDMS analysis, and in combination with the mass spectrum data, it is speculated that the molecular formula of the compound is C 16 H 12 O6.

[0056] The mother liquor is subjected to HNMR analysis, and the chemical structure of the compound is finally determined as shown in Formula I.

[0057] The mother liquor is subjected to separation and purification, and the operation of the purification is preferably as follows:

[0058] The mother liquor is filtered; silica gel is added to the filtrate, and then vacuum concentration is performed to obtain a stationary phase; the stationary phase is subjected to gradient elution using dichloromethane and methanol as the developing agent, and the elution phase with a gradient of dichloromethane:methanol=15:1 to dichloromethane:methanol=5:1 is collected to obtain a crude product; the crude product is subjected to vacuum concentration to obtain the flavonoids of the above Formula I. In the above purification process, the first vacuum concentration is used to remove the solvent ethanol to obtain a solid precipitate; the second vacuum concentration is used to remove the eluent from the solution obtained after chromatographic separation to obtain the pure flavonoids product. The temperature of the vacuum concentration is preferably 45-55°C to avoid the damage to the flavonoids caused by high temperature. As a preferred scheme of the present embodiment, the mass ratio of the above mother liquor to silica gel is preferably (10-18):1; the gradient elution range of the developing agent is preferably dichloromethane:methanol=100:1 to dichloromethane:methanol=1:1.

[0059] The product after purification is subjected to HPLC, LC-MS, HDMS and HNMR analysis, and it is determined that it is successfully separated from the mother liquor, and the structure is the compound of the above Formula I.

[0060] Another embodiment of the present application also provides a flavonoid compound with a molecular formula of C 16 H 12 O6, and a structure as shown in formula I:

[0061] The flavonoid compound can be prepared according to the above method, and the specific embodiment manner is the same as the foregoing embodiment, and thus will not be described herein.

[0062] From the above, the flavonoid compound and the preparation method thereof provided by the embodiment of the present application have the following advantages:

[0063] 1) The structure of the bacteriostatic effective component in the extract of G. stellatum is determined to be the flavonoid compound as shown in formula I, and a preparation method of the flavonoid compound is correspondingly provided, thereby improving the biological activity and application value of the extract, ensuring the consistency and repeatability of the extract, and providing a natural active substance with high efficiency and stability for the antibacterial field.

[0064] 2) The preparation method first performs freeze-drying before ultrasonic extraction of the G. stellatum after removing the spines, thereby reducing the viscosity of the extraction system, making the extraction process more uniform, and improving the consistency and repeatability of the extraction; in addition, the freeze-drying process is performed at low temperature, thereby avoiding the damage of high temperature to the flavonoid compound and ensuring the yield of the flavonoid compound.

[0065] 3) In the preparation method, the extraction liquid of the ultrasonic extraction is an alcohol solution, and the purity of the flavonoid compound in the mother liquor is higher, thereby reducing the difficulty of subsequent purification of the flavonoid compound.

[0066] Another embodiment of the present application also provides an application of the above-mentioned flavonoid compound in a bacteriostatic product, and the bacteriostatic product includes one or more of the following: cosmetics, drugs, health products, and food additives.

[0067] The technical solutions of the present application will be further described below in combination with specific embodiments:

[0068] Embodiment 1

[0069] 1) Take G. stellatum, remove the spines, and freeze-dry the G. stellatum after removing the spines as a whole, and the freeze-drying temperature is-30℃, and the freeze-drying time is 48h.

[0070] 2) After freeze-drying, use a mortar to grind to obtain G. stellatum powder.

[0071] 3) Take 2g of G. stellatum powder, add 60ml of 80% ethanol water (volume ratio), and ultrasonically extract at 55℃ for 2.5h, and then filter with a 0.45μm organic membrane to obtain 50.87g of extract mother liquor.

[0072] 4. Isolation and purification: The filtered ethanol solution 50.78 g was added 4.5 g silica gel, and concentrated under reduced pressure, the concentration temperature was 50 °C, to get solid, and then the solid was separated by normal phase, the developing agent gradient was dichloromethane: methanol = 100: 1 to 1: 1, the product gradient was dichloromethane: methanol = 15: 1 to 5: 1, and the obtained product was concentrated under reduced pressure, the concentration temperature was 50 °C, to get product 0.81 g.

[0073]

Qualitative analysis

[0074] The mother liquor obtained in step 3 and the product obtained in step 4 were analyzed by HPLC, and the HPLC analysis conditions were as follows:

[0075] Analysis equipment: Hitachi full wavelength liquid phase analysis system

[0076] Mobile phase: A-0.2% formic acid, B-methanol

[0077] Chromatographic column: C18, 5 μm, 4.6*150 mm

[0078] Detection conditions: detection wavelength 360 nm, column temperature 40 °C, injection volume 20 μl, flow rate 1.2 ml / min

[0079] The mobile phase gradient conditions are shown in Table 1:

[0080] Table 1 Mobile phase gradient conditions

[0081] The HPLC liquid chromatograms of the mother liquor and the product of the gold ball extract are shown in Figures 1 and 2 respectively, and the results of the liquid phase analysis are shown in Table 2:

[0082] Table 2 HPLC analysis results

[0083] As can be seen from Figures 1-2 and Table 2, the purity of the mother liquor is 43.18%, and the purity of the gold ball extract product after normal phase purification reaches 99.75%, thereby achieving the effect of product purification.

[0084] Through HPLC analysis, it can be found that the main chromatographic peak of the mother liquor is near 23.1 min. The product was further studied by LC-MS on the molecular weight of the chromatographic peak.

[0085] LC-MS analysis conditions are as follows:

[0086] Analysis equipment: mass spectrometer of waters

[0087] Mobile phase: same as HPLC

[0088] Chromatographic column: same as HPLC

[0089] Detection condition: same as HPLC

[0090] Gradient condition of mobile phase: same as HPLC

[0091] The LC-MS analysis spectrum of the product is shown in Figure 3, and it can be seen from the figure that the molecular weight of the compound corresponding to the chromatographic peak at 23.1 min should be about 300.3.

[0092] The product of the gold chrysanthemum extract was further analyzed by high resolution mass spectrometry (HDMS). By increasing the sample injection amount of the liquid phase system, the chromatographic peak at a retention time of 23 min was manually collected and further analyzed by high resolution mass spectrometry to speculate the possible molecular formula. The negative ion mode mass spectrum and the positive ion mode mass spectrum are shown in Figures 4 and 5, respectively.

[0093] The high resolution mass spectrometry negative ion mode mass spectrum shows that the accurate molecular weight of the M-1 molecular ion peak is 299.0551, and the accurate molecular weight of the M+1 molecular ion peak is 301.0707. Referring to the structural characteristics of flavones, the element composition is limited to CHO, and the most possible molecular formula is C 16 H 12 O6.

[0094] The gold chrysanthemum extract product was analyzed by nuclear magnetic hydrogen spectrum (HNMR). The HNMR analysis spectrum is shown in Figure 6.

[0095] From the combination of HNMR and HDMS, it can be seen that the deviation between the actual value and the theoretical value of the positive and negative ions is far less than 10 ppm, and the matching degree is very high. It is judged that the structure shown in formula I:

[0096] Example 2

[0097] The difference from Example 1 is only that:

[0098] The ultrasonic temperature in step 3 is 45°C, and the ultrasonic time is 2.5 hours. 50.11 g of extract mother liquor is obtained.

[0099] Example 3

[0100] The difference from Example 1 is only that:

[0101] The ultrasonic temperature in step 3 is 55°C, and the ultrasonic time is 1.5 hours. 50.03 g of extract mother liquor is obtained.

[0102] From Examples 1-3, it can be seen that when the ultrasonic alcohol extraction temperature is 55°C and the time is 2.5h, the yield of the mother liquor is the highest, and accordingly the yield of flavonoids is the highest.

[0103] Comparative Example 1

[0104] The difference from Example 1 is only that:

[0105] The extraction liquid in Step 3 is 60 ml of water, and 60.78 g of extract mother liquor is obtained.

[0106] The extract mother liquor of Comparative Example 1 is subjected to HPLC respectively, and the HPLC spectrum is shown in Figure 7, and the analysis results are shown in Table 3. From Figure 1 and Figure 7, Table 2 and Table 3, it can be seen that the purity of flavonoids in the extract mother liquor obtained by alcohol extraction is higher.

[0107] Table 3 HPLC analysis results

[0108]

Bacteriostatic experiment

[0109] (I) Experimental steps

[0110] 1. Sample preparation: In the clean bench, weigh 3 portions of the extract of the gold-containing plant prepared in Example 1, each 3 g; add 1000 g, 200 g and 100 g of sterile water respectively, vortex for 1 minute, place the centrifuge tube in the ultrasonic cleaner for ultrasonic dissolution, centrifuge at 4500 rpm for 30 minutes, take the supernatant and filter it through a 0.22 micron disposable sterile filter. The sample is the sample to be tested, and the information of the sample to be tested is shown in Table 4. The sample to be tested is stored at 4°C.

[0111] Table 4 Information of the sample to be tested

[0112] 2. Preparation of indicator bacterial suspension

[0113] Strain activation: the previous day, use LB medium and modified Martin medium to inoculate the test strains (Staphylococcus epidermidis and Malassezia), the inoculation ratio is 1:1000, and the Staphylococcus epidermidis and Malassezia are cultured at 36°C±1°C, 150 rpm for 18-24h.

[0114] Preparation of bacterial suspension: measure the absorbance at 600 nm of the activated bacterial solution, and dilute it with LB medium and modified Martin medium to OD600 of about 0.6±0.05 (i.e. the concentration of the bacterial suspension is 10 8 CFU / mL)

[0115] Preparation of detection plate: heat and dissolve the prepared NA medium and modified Martin agar medium, cool to 55-60°C (can be incubated in a water bath), add the prepared indicator bacterial suspension to the medium according to an addition amount of 0.1%, mix well, pour into a sterile plate, gently shake the plate to evenly spread, and wait for it to solidify for standby.

[0116] 3. Measurement

[0117] Sample determination: 5 holes were punched in the detection plate containing the indicator bacteria with 1 mL sterile gun head, 100 microliters of sample was measured, and slowly added to the hole, and the plate for culturing Staphylococcus epidermidis and Malassezia was placed in a 36℃±1℃ constant temperature incubator, and incubated vertically until the bacteriostatic ring was clear, and the diameter of the bacteriostatic ring was measured with a caliper.

[0118] Control determination: sterile water was taken as a blank control, and 100 mg / mL ampicillin was taken as a positive control. The steps were the same as the sample determination.

[0119] (II) Experimental results

[0120] The bacteriostatic effect of the gold chrysanthemum extract product prepared in Example 1 on Staphylococcus epidermidis and Malassezia is shown in Figure 8, where "A + " represents ampicillin (positive control), "A + " represents, "H2O" represents sterile water (negative control), "10" represents gold chrysanthemum extract (10 mM), "50" represents gold chrysanthemum extract (50 mM), and "100" represents gold chrysanthemum extract (100 mM). The bacteriostatic effect data are shown in Tables 5 and 6, respectively.

[0121] Table 5 Bacteriostatic effect of gold chrysanthemum extract product on Staphylococcus epidermidis

[0122] Table 6 Bacteriostatic effect of gold chrysanthemum extract product on Malassezia

[0123] From the results of the bacteriostatic experiment, it can be seen that the purified gold chrysanthemum extract (i.e., the flavonoid compound shown in Formula I above) has good bacteriostatic effect; and as the concentration of the gold chrysanthemum extract increases, the bacteriostatic effect also increases.

[0124] The above has described various embodiments of the present application, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes are obvious to those skilled in the art without departing from the scope and spirit of the described embodiments. The choice of terms used herein is intended to best explain the principles, practical applications, or improvements to the technology in the market of the embodiments, or to enable other ordinary skilled persons in the art to understand the embodiments disclosed herein.

Claims

1. A method for preparing a flavonoid compound, characterized by, The application relates to a method for extracting and purifying flavonoids from gold echinocactus. The gold echinocactus is ground after being defleshed and freeze-dried to obtain gold echinocactus powder; The gold echinocactus powder is subjected to ultrasonic alcohol extraction and organic membrane filtration to obtain an extract mother liquor; The mother liquor is separated and purified to obtain the flavone compound shown in formula I:

2. The production method according to claim 1, characterized by, The freeze-drying temperature is-40 to-20 DEG C, and the time is 42 to 72 hours.

3. The production method according to claim 1, characterized by, The ultrasonic alcohol extraction temperature is 45 to 55 DEG C, and the time is 1.5 to 3.0 hours.

4. The method of claim 1, wherein, The ultrasonic alcohol extraction extract liquid is an 80% ethanol solution, and the solid-liquid ratio of the gold echinocactus powder and the ethanol solution is 1g:(25 to 45)mL.

5. The preparation method according to claim 1, characterized in that, The organic membrane has a pore size of 0.45 microns.

6. The method of claim 1, wherein, The separation and purification are specifically as follows: the mother liquor is filtered; silica gel is added into the filtrate, and then vacuum concentration is carried out to obtain a stationary phase; dichloromethane and methanol are used as developing agents to carry out gradient elution on the stationary phase, and the elution phase with a gradient of dichloromethane:methanol=15:1 to dichloromethane:methanol=5:1 is collected to obtain a crude product; the crude product is subjected to vacuum concentration to obtain the flavonoids shown in formula I.

7. The preparation method according to claim 7, characterized in that, The vacuum concentration temperature is 45 to 55 DEG C.

8. The method of claim 7, wherein, The mass ratio of the mother liquor to the silica gel is (10 to 18):1; and the gradient elution range of the developing agent is dichloromethane:methanol=100:1 to dichloromethane:methanol=1:

1.

9. A flavonoid compound, characterized in that, The structure is shown in formula I: 10.The flavonoids shown in claim 9 are applied to bacteriostatic products, and the bacteriostatic products include one or more of cosmetics, medicines, health products and food additives.

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