Treatment of hair to strengthen and retain shape of hair

Abstract

Methods and compositions for treating hair to strengthen hair and / or retain and / or restore shape of the hair disclosed herein. The methods and compositions use PB-300 peptide, hydrogen peroxide, and / or bis-aminopropyl diglycol dimaleate. The methods may use multiple steps. The methods and compositions may increase curl retention, increase strength to reduce breakage of the hair, decrease tangling, and / or reduce hair frizziness and / or hair fly-aways without decreasing tensile strength.

Description

PATENTOLAP.P0009WOPCT PATENT APPLICATION forTREATMENT OF HAIR TO STRENGTHEN AND RETAIN SHAPE OF HAIR byMatthew CORDESMona VAIDYA andLavinia POPESCU299472458.1 - 1 - OLAP.POOQ9WO / 1001359125CROSS-REFERENCE TO RELATED PATENT APPLICATIONS

[0001] This application claims the benefit of United States Provisional Patent Application No. 63 / 687,082, filed August 26, 2024. The contents of the referenced patent application is incorporated into the present application by reference.FIELD OF THE INVENTION

[0002] This invention general relates to the field of personal care methods and products that include hair treatments. In one aspect, the invention includes a multi-step process of applying a peptide containing composition to hair followed with in situ oxidization of the peptide, which can have beneficial results to the hair (e.g., curl retention, stronger hair, decreased tangling, and / or reduced hair frizziness or hair fly-aways). In one aspect, the invention includes applying one or more than one composition to hair to treat a hair, which can have beneficial results to the hair (e.g., curl retention, stronger hair, decreased tangling, and / or reduced hair frizziness or hair fly-aways).BACKGROUND OF THE INVENTION

[0003] Hair care and styling products can be useful to improve the appearance of hair. For instance, such products can create the appearance of more voluminous hair, more shiny hair, and / or can change the shape of hair (e.g., introducing curls or straightening hair). Some known methods to introduce these appearances in hair include heat treatment, color treatment, chemical treatment, and / or exposing hair to ultraviolet light or radiation.. While these treatments can provide for a desired appearance, the treated hair oftentimes becomes damaged and can suffer from dryness, brittleness or reduced strength, increased rough surface texture, loss of color, loss of natural shape, decreased luster, and / or stiffness.

[0004] There have been attempts to mitigate damage to hair from hair treatment processes (e.g., introduction of curls, straightening hair, color change, etc.). In one example, compositions formulated as solutions, foams, gels, or emulsions can oftentimes include ingredients to help treat the damaged or processed hair. Unfortunately, these compositions can result in greasy hair, weighed down hair, and / or sticky or tacky hair. Ultimately, current hair treatment formulations used to mitigate damage to hair processing techniques may exacerbate the hair damage and / or have a negative effect on the tactile properties of the hair.SUMMARY OF THE INVENTION

[0005] A solution has been identified to at least one or more of the aforementioned problems associated with hair treatment processes. In one aspect, the solution can include a299472458.1 - 2 - OLAP.POOQ9WO / 1001359125multi-step process of (1) applying a non-oxidized active agent to the hair (e.g., PB-300 peptide) and (2) in situ oxidation (e.g., with hydrogen peroxide) of the active agent (e.g., PB-300 peptide) in the hair. With wishing to be bound by theory, it is believed that in situ oxidation of the active agent while the active agent is in contact with the hair can activate the active agent so as to immediately perform its beneficial hair care properties as soon as it becomes activated. By comparison, it is believed that oxidation of the active agent followed by subsequent application of the oxidized active agent to the hair can potentially cause reduced efficacy of the active agent through degradation of the oxidized active agent prior to being applied to hair. It is also believed that in situ oxidation of the active agent can possibly also result in oxidation of at least a portion of the hair; however, in some instances, less amount of agent used to oxidize the active agent is used in comparison to an amount of agent used to oxidize hair when bleaching hair, straightening curly hair, and / or curl straight hair, etc. In another aspect, the process of the present invention can include at least a third step (3), which includes application of a hair binding agent (e.g., bis-aminopropyl diglycol dimaleate) to the hair. Step (3) can be performed during or after step (2) is performed. In additional to the increased effectiveness / reduced degradation of the active agent, the two and / or three step process of the present invention can increase curl retention, increase strength to reduce breakage of the hair, decrease tangling, and / or reduce hair frizziness and / or hair fly-aways, without decreasing hair tensile strength. In one aspect, the solution can include application of the third composition to the hair. In some aspects, the compositions and / or methods herein (1) increase curl retention in hair; (2) increase strength of the hair to reduce breakage of the hair; (3) not negatively effect the hair shafts tensile strength; (4) decrease hair tangling; and / or (5) reduce the appearance of hair frizziness and / or hair fly-aways.

[0006] In some instances, a first composition containing PB-300 peptide is disclosed. The first composition may contain PB-300 peptide and any other ingredient disclosed herein at a concentration of 0.00000001 to 99.99999999 wt. % based on the weight of the first composition. In some instances, the first composition contains the PB-300 peptide at 0.00001 to 1 wt. % by weight of the first composition, such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74,299472458.1 - 3 - OLAP.POOQ9WO / 10013591250.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %.

[0007] In some instances, the first composition contains along with the PB-300 peptide or instead of the PB-300 peptide a peptide described in U.S. Patent Number 11,596,589, titled “Compositions and Method of Use for Hair Straightening and Shaping,” which patent is hereby incorporated by reference and / or a peptide with a sequences having at least one amino acid difference from SEQ ID NO: 1 and typically capable of binding to keratin. In some instances, any one or more of the amino acids in the amino acid sequence of SEQ ID NO: 1, can be replaced or substituted with cysteine.

[0008] In some examples, the total cysteine content of a disclosed peptide is less than 50% of SEQ ID NO. 1, less than 45% of SEQ ID NO. 1, less than 40% of SEQ ID NO. 1, less than 35% of SEQ ID NO. 1, less than 30% of SEQ ID NO. 1, less than 25% of SEQ ID NO. 1, less than 20% of SEQ ID NO. 1, less than 15% of SEQ ID NO. 1, less than 10% of SEQ ID NO. 1, less than 9% of SEQ ID NO. 1, less than 8% of SEQ ID NO. 1, less than 7% of SEQ ID NO. 1, less than 6% of SEQ ID NO. 1, or less than 5% of SEQ ID NO. 1.

[0009] The peptides provided and / or the PB-300 can be used individually or in combination in a hair composition such as those disclosed herein, known in the art, or designed by one of skill in the art. Fragments and variants can be used, such as those that have, for example, at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% sequence identity to SEQ ID NO: 1. The variants can include one or more amino acid substitution(s), addition(s), deletion(s), or a combination thereof relative to the reference peptide. The peptides typically include at least 1, preferably 2, 3, or more cysteines.

[0010] In some embodiments, the peptide is 10-50 amino acids in length, or any subrange there between inclusive, or any specific integer there between inclusive, including, but not limited to 10-45, 10-40, 10-35, 10-30, 10-29, 10-28, 10-27, 10-26, 10-25, 10-24, 10-23, 10-22, 10-21, 10-20, 10-19, 10-18, 10-17, 10-16, 10-15, 15-20, 15-21, 15-22, 15-23, 15-24, 15-25, 15- 26, 15-27, 15-28, 15-29, 15-30, 18-27, 19-26, 20-25, or 21-24 amino acids inclusive, or 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids.

[0011] Typically, the peptides can bind to hair and / or a keratin protein thereof. Binding and / or retention to hair can be demonstrated by measuring resistance to washing. Retention of the peptide(s) can be measured, for example, by fluorescently labelling the peptide(s) and applying them to hair or keratin protein thereof and allowing the labelled peptides to bind to the hair and / or keratin protein thereof and subsequently subjecting the hair to repeated washings (e.g., shampoos). Comparison and statistical analysis of fluorescence imaging of the299472458.1 - 4 - OLAP.POOQ9WO / 1001359125labeled peptides on the hair prior to washing and following at least one, 5, 10, 15, 20, 25, or 30 washings allows for determination of the degree of retention of the peptide(s) to the hair. In some embodiments, the hair is damaged hair. For example, in some embodiments, prior to testing binding and / or retention to the hair, the hair is damaged, such as by subjecting the hair to repeated bleaching treatments.

[0012] In some instances, the first composition further comprises one or more additional ingredient. The one or more additional ingredients may include water, preservatives, antioxidants, softeners, chelating agents, sunscreen agents, vitamins, dyes, hair coloring agents, pigments, fillers, proteins, amino acids, natural extracts such as plant extracts, moisturizing agents, humectants, fragrances, perfumes, oils such as essential oils or natural oils, emollients, surfactants, lubricants, butters, penetrants, thickeners, viscosity modifiers, polymers, resins, hair fixatives, film formers, surfactants, detergents, emulsifiers, opacifying agents, volatiles, propellants, foaming agents, antifoaming agents, liquid vehicles, carriers, salts, pH adjusting agents, neutralizing agents, buffers, pharmaceutically-active agents, hair conditioning agents, anti-static agents, anti-frizz agents, anti-dandruff agents, absorbents, and combinations thereof. The one or more additional ingredients may be any of the ingredients disclosed herein. In some instances, the one or more additional ingredients is one or more of hydroxyethylcellulose, glycerin, phenoxyethanol, caprylyl glycol, chlorphenesin, and / or trisodium ethylenediamine di succinate.

[0013] In some instances, the first composition contains 0.01 to 1.5 wt. % hydroxy ethylcellulose, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the first composition. In some instances, the first composition contains 0.1 to 5 wt. % glycerin, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, or 5 wt. % based on the weight of the first composition. In some instances, the first composition contains 0.01 to 1.5 wt. % phenoxyethanol, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the first composition. In some instances, the first composition contains 0.01 to 1.5 wt. % caprylyl glycol, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the first composition. In some instances, the first299472458.1 - 5 - OLAP.POOQ9WO / 1001359125composition contains 0.001 to 1 wt. % chlorphenesin, such as equal to, more than, less than, a range of, or between 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 wt. % based on the weight of the first composition. In some instances, the first composition contains 0.001 to 1 wt. % trisodium ethylenediamine disuccinate, such as equal to, more than, less than, a range of, or between 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 wt. % based on the weight of the first composition.

[0014] In some instances, a second composition containing hydrogen peroxide is disclosed. The second composition may contain hydrogen peroxide and any other ingredient disclosed herein at a concentration of 0.00000001 to 99.99999999 wt. % based on the weight of the second composition. In some instances, the second composition contains the hydrogen peroxide at 0.005 to 2.5 wt. % by weight of the second composition, such as equal to, more than, less than, a range of, or between 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, or 2.5 wt. %.

[0015] In some instances, the second composition further comprises one or more additional ingredient. The one or more additional ingredients may include water, preservatives, antioxidants, softeners, chelating agents, sunscreen agents, vitamins, dyes, hair coloring agents, pigments, fillers, proteins, amino acids, natural extracts such as plant extracts, moisturizing agents, humectants, fragrances, perfumes, oils such as essential oils or natural oils, emollients, surfactants, lubricants, butters, penetrants, thickeners, viscosity modifiers, polymers, resins, hair fixatives, film formers, surfactants, detergents, emulsifiers, opacifying agents, volatiles, propellants, foaming agents, antifoaming agents, liquid vehicles, carriers, salts, pH adjusting agents, neutralizing agents, buffers, pharmaceutically-active agents, hair conditioning agents, anti-static agents, anti-frizz agents, anti-dandruff agents, absorbents, and combinations thereof. The one or more additional ingredients may be any of the ingredients disclosed herein. In some instances, the one or more additional ingredients is one or more of propanediol, caprylyl / capryl glucoside, and / or sodium cocoyl glutamate.

[0016] In some instances, the second composition contains 0.1 to 5 wt. % propanediol, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9,299472458.1 - 6 - OLAP.POOQ9WO / 10013591253.0 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, or 5 wt. % based on the weight of the second composition. In some instances, the second composition contains 0.01 to 1.5 wt. % caprylyl / capryl glucoside such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the second composition. In some instances, the second composition contains 0.001 to 1 wt. % sodium cocoyl glutamate such as equal to, more than, less than, a range of, or between 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 wt. % based on the weight of the second composition.

[0017] In some instances, a third composition containing the bis-aminopropyl diglycol dimaleate is disclosed. In some instances, the third composition is a stand alone composition that is not used in the methods disclosed herein and / or is not used in the same treatment as one or more of the first composition and second composition. The third composition may contain bis-aminopropyl diglycol dimaleate and any other ingredient disclosed herein at a concentration of 0.00000001 to 99.99999999 wt. % based on the weight of the third composition. In some instances, the third composition contains the bis-aminopropyl diglycol dimaleate at 0.00001 to 1 wt. % by weight of the third composition, such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %.

[0018] In some instances, the third composition contains along with the bis-aminopropyl diglycol dimaleate or instead of the bis-aminopropyl diglycol dimaleate a compound of Formula I299472458.1 - 7 - OLAP.POOQ9WO / 1001359125Formula I wherein:A, B, C, and D are reactive moieties capable of reacting with the free thiol groups and contain one or more charges, and wherein each of A, B, C, and D independently comprises a vinyl sulfone, an acrylate group, a methacrylate group, a styrene group, an acryl amide group, a methacryl amide group, a maleate group, a fumarate group, and / or an itaconate group; andR is a linker that contains two or more charges, wherein the charges are opposite to the charges on the reactive moieties, wherein n=l-10, and wherein R is not a polymer, wherein each occurrence of p, q, r, and s is independently an integer from 0 to 25, and wherein the sum of p+q+r+s is equal to or greater than 2. In some instances, the sum of the charges of the compound of Formula I is zero. In some instances, the reactive moieties are ionically bound to the linker. In some instances, the third composition contains the compound of Formula I at 0.00001 to 1 wt. % by weight of the third composition, such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %.

[0019] In some instances, the third composition further comprises one or more additional ingredient. In some instances, the third composition excludes one or more of the additional ingredient. The one or more additional ingredients may include water, preservatives, antioxidants, softeners, chelating agents, sunscreen agents, vitamins, dyes, hair coloring agents, pigments, fillers, proteins, amino acids, natural extracts such as plant extracts, moisturizing agents, humectants, fragrances, perfumes, oils such as essential oils or natural oils, emollients, surfactants, lubricants, butters, penetrants, thickeners, viscosity modifiers, polymers, resins, hair fixatives, film formers, surfactants, detergents, emulsifiers, opacifying agents, volatiles, propellants, foaming agents, antifoaming agents, liquid vehicles, carriers, salts, pH adjusting agents, neutralizing agents, buffers, pharmaceutically-active agents, hair conditioning agents, anti-static agents, anti-frizz agents, anti-dandruff agents, absorbents, and combinations thereof. The one or more additional ingredients may be any of the ingredients disclosed herein. In some299472458.1 - 8 - OLAP.POOQ9WO / 1001359125instances, the one or more additional ingredients is one or more of xylitylglucoside, anhydroxylitol, hydroxyethylcellulose, phenoxyethanol, maltitol, glycerin, caprylyl / capryl glucoside, xylitol, sodium polyacryloyldimethyl taurate, PVM / MA copolymer, ethylhexylglycerin, polyimide- 1, propanediol, guar hydroxypropyltrimonium chloride, hydroxypropyl starch phosphate, aspergillus ferment, and / or Acacia senega! gum. In some instances, the third composition excludes sodium polyacryloyldimethyl taurate and / or PVM / MA copolymer.

[0020] In some instances, the third composition contains 0.1 to 5 wt. % xylitylglucoside, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, or 5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.1 to 5 wt. % anhydroxylitol, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2,2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, or 5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.1 to 5 wt. % hydroxyethylcellulose, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0 3.1, 3.2, 3.3,3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, or 5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.1 to 5 wt. % phenoxyethanol, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4,2.5, 2.6, 2.7, 2.8, 2.9, 3.0 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6,4.7, 4.8, 4.9, or 5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 3 wt. % maltitol, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7,2.8, 2.9, or 3 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 3 wt. % glycerin, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8,2.9, or 3 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 2 wt. % caprylyl / capryl glucoside, such as equal to, more than,299472458.1 - 9 - OLAP.POOQ9WO / 1001359125less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 2 wt. % xylitol, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 1.5 wt. % sodium polyacryloyldimethyl taurate, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 1.5 wt. % PVM / MA copolymer, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.001 to 1 wt. % ethylhexylglycerin, such as equal to, more than, less than, a range of, or between 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.001 to 2 wt. % polyimide-1, such as equal to, more than, less than, a range of, or between 0.001, 0.002, 0.003, 0.004, 0.005, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2,1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.1 to 3 wt. % propanediol, such as equal to, more than, less than, a range of, or between 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2,1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.05 to 2 wt. % guar hydroxypropyltrimonium chloride, such as equal to, more than, less than, a range of, or between 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2,1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.05 to 2 wt. % hydroxypropyl starch phosphate, such as equal to, more than, less than, a range of, or between 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2 wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 1 wt. % aspergillus ferment, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1299472458.1 - 10 - OLAP.POOQ9WO / 1001359125wt. % based on the weight of the third composition. In some instances, the third composition contains 0.01 to 1 wt. % Acacia senega! gum, such as equal to, more than, less than, a range of, or between 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, or 1 wt. % based on the weight of the third composition.

[0021] In some instances, a single composition contains all or a portion of the ingredients of the first composition and the second composition, the first composition and the third composition, the second composition and the third composition, and the first composition, the second composition, and the third composition. As a non-limiting example, a composition may contain both the PB-300 peptide and the bis-aminopropyl diglycol dimaleate. As a nonlimiting example, a composition may contain both the PB-300 peptide and the hydrogen peroxide. As a non-limiting example, a composition may contain both the bis-aminopropyl diglycol dimaleate and the hydrogen peroxide. As a non-limiting example, a composition may contain the PB-300 peptide, the bis-aminopropyl diglycol dimaleate, and the hydrogen peroxide. In some instances, the single composition may contain these ingredients and any other ingredient disclosed herein at a concentration of 0.00000001 to 99.99999999 wt. % based on the weight of the single composition. In some instances, the single composition contains the PB-300 peptide, the bis-aminopropyl diglycol dimaleate, and / or the hydrogen peroxide at PB-300 peptide at 0.00001 to 1 wt. % by weight of the single composition, such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34,0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51,0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85,0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %, the hydrogen peroxide at 0.005 to 2.5 wt. % by weight of the second composition, such as equal to, more than, less than, a range of, or between 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, or 2.5 wt. %, and / or the bis-aminopropyl diglycol dimaleate at 0.00001 to 1 wt. % by weight of the third composition, such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21,299472458.1 - 11 - OLAP.POOQ9WO / 10013591250.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %.

[0022] In certain embodiments, the first composition, second composition, and / or third composition, or a combination thereof may also be used independently on a person’s hair, e.g., independently of use of the other compositions and / or independently of the methods disclosed herein.

[0023] In certain embodiments, the first composition, second composition, and / or third composition, or a combination thereof is comprised in a container. The container can be a bottle, dispenser, or package. The container can dispense a pre-determined amount of the respective composition. In certain aspects, the respective compositions is dispensed in a spray, mist, dollop, or liquid. The container can include indicia on its surface. The indicia can be a word, an abbreviation, a picture, or a symbol.

[0024] In some aspects, methods of treating hair are disclosed herein. The methods may include applying any one of or a combination of the first composition, the second composition, and the third composition to hair. The method may include applying the first composition and the second composition to hair. The method may include applying the first composition and the third composition to hair. The method may include applying the second composition and the third composition to hair. The method may include applying the first composition, the second composition, and the third composition to hair. The method may include applying only the first composition to the hair. The method may include applying only the second composition to the hair. The method may include applying on the third composition to the hair. The method may include applying these compositions separately or together to the hair. The method may include applying these compositions in any order to the hair. The method may include applying these compositions to hair without rinsing any one of or a combination of the compositions out of the hair.

[0025] In some instances, the method increases curl retention, increases strength to reduce breakage of the hair, decreases tangling, and / or reduces hair frizziness and / or hair fly-aways, without decreasing hair tensile strength when compared to hair that has not been subjected to the method.

[0026] In some instances, the method includes applying the first composition to the hair and applying the second composition to the hair, wherein the hydrogen peroxide oxidizes at299472458.1 - 12 - OLAP.POOQ9WO / 1001359125least a portion of the PB-300 peptide and / or the hair and the hair is at least partially coated with oxidized PB-300 peptide. In some instances, the method includes further applying the third composition to the hair at least partially coated with oxidized PB-300 peptide.

[0027] In some instances, the second composition is applied to the hair within 2 hours of the first composition being applied to the hair, such as equal to, more than, less than, a range of, or between 2 hours, 1 hour and 55 minutes, 1 hour and 50 minutes, 1 hour and 45 minutes, 1 hour and 40 minutes, 1 hour and 35 minutes, 1 hour and 30 minutes, 1 hour and 25 minutes,1 hour and 20 minutes, 1 hour and 15 minutes, 1 hour and 10 minutes, 1 hour and 5 minutes, 1 hour, 55 minutes, 50 minutes, 45 minutes, 40 minutes, 35 minutes, 30 minutes, 25 minutes, 20 minutes, 15 minutes, 10 minutes, or 5 minutes of the first composition being applied to the hair. In some instances, the third composition is applied to the hair within 3 hours of the first composition being applied to the hair, such as equal to, more than, less than, a range of, or between 3 hours, 2 hours and 55 minutes, 2 hours and 50 minutes, 2 hours and 45 minutes, 2 hours and 40 minutes, 2 hours and 35 minutes, 2 hours and 30 minutes, 2 hours and 25 minutes,2 hours and 20 minutes, 2 hours and 15 minutes, 2 hours and 10 minutes, 2 hours and 5 minutes, 2 hours, 1 hour and 55 minutes, 1 hour and 50 minutes, 1 hour and 45 minutes, 1 hour and 40 minutes, 1 hour and 35 minutes, 1 hour and 30 minutes, 1 hour and 25 minutes, 1 hour and 20 minutes, 1 hour and 15 minutes, 1 hour and 10 minutes, 1 hour and 5 minutes, 1 hour, 55 minutes, 50 minutes, 45 minutes, 40 minutes, 35 minutes, 30 minutes, 25 minutes, 20 minutes, 15 minutes, 10 minutes, or 5 minutes of the first composition being applied to the hair. In some instances, the third composition is applied to the hair within 2 hours of the second composition being applied to the hair, such as equal to, more than, less than, a range of, or between 2 hours, 1 hour and 55 minutes, 1 hour and 50 minutes, 1 hour and 45 minutes, 1 hour and 40 minutes, 1 hour and 35 minutes, 1 hour and 30 minutes, 1 hour and 25 minutes, 1 hour and 20 minutes, 1 hour and 15 minutes, 1 hour and 10 minutes, 1 hour and 5 minutes, 1 hour, 55 minutes, 50 minutes, 45 minutes, 40 minutes, 35 minutes, 30 minutes, 25 minutes, 20 minutes, 15 minutes, 10 minutes, or 5 minutes of the first composition being applied to the hair.

[0028] In some instances, the first composition, second composition, third composition or combination thereof stays on the hair without being rinsed off for at least 2 hours, such as equal to, more than, less than, a range of, or between 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 hours. In some instances, the first composition is present on the hair for at least 5 minutes before the second composition and / or third composition is applied to the hair, such as equal to, more than, less than, a range of, or between 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes,299472458.1 - 13 - OLAP.POOQ9WO / 100135912540 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, or 1 hour and 55 minutes. In some instances, the second composition is present on the hair for at least 5 minutes before the third composition is applied to the hair, such as equal to, more than, less than, a range of, or between 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, 1 hour and 55 minutes, 2 hours, 2 hours and 5 minutes, 2 hours and 10 minutes, 2 hours and 15 minutes, 2 hours and 20 minutes, 2 hours and 25 minutes, 2 hours and 30 minutes, 2 hours and 35 minutes, 2 hours and 40 minutes, 2 hours and 45 minutes, 2 hours and 50 minutes, or 2 hours and 55 minutes. In some instances, the third composition stays on the hair without being rinsed off for at least 2 hours, such as equal to, more than, less than, a range of, or between 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 hours.

[0029] The method can be applied to any type of hair, such as straight, wavey, curly, crimpy, black, white, silver, grey, brown, auburn, red, blond, dyed, color treated, permed, straitened, and / or heat treated hair. In some instances, the hair is straight, curly, wavey, or crimpy hair that is naturally straight, curly, wavey, or crimpy. In some instances, the hair is naturally curly, wavey, or crimpy hair and the method increases, restores, and / or protects the curly, wavey, or crimpy shape of the hair. In some instances, the method increases, restores, and / or protects the curly, wavey, or crimpy shape of the hair for at least 18 hours, such as equal to, more than, less than, a range of, or between 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 hours, or 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or 21 days.

[0030] The method may further include styling the hair after application of the first, second, and / or third composition. The method may improve the retention of the styling of the hair. In some instances, the method improves the retention of the styling of the hair for at least 18 hours, such as equal to, more than, less than, a range of, or between 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, or 48 hours, or 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, or 21 days. The method may include styling the hair within 3 hours of applying the first, second, and / or third composition to the hair, such as equal to, more than, less than, a range of, or between 5 minutes,299472458.1 - 14 - OLAP.POOQ9WO / 100135912510 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, or 1 hour and 55 minutes, 2 hours, 2 hours and 5 minutes, 2 hours and 10 minutes, 2 hours and 15 minutes, 2 hours and 20 minutes, 2 hours and 25 minutes, 2 hours and 30 minutes, 2 hours and 35 minutes, 2 hours and 40 minutes, 2 hours and 45 minutes, 2 hours and 50 minutes, 2 hours and 55 minutes, or 3 hours.

[0031] In some instances, the method does not change the color of the hair. In some instances, the method does not change the color of the hair once the hair is dried as compared to the hair before the method, or compared to the hair not subject to the method. The color of the hair and / or change in color of the hair may be determined by unaided human vision. The color of the hair that is not changed may be the natural color of the hair, the dyed color of the hair, the color treated color of the hair, and / or the bleached color of the hair.

[0032] In some aspects, disclosed herein is a kit that contains one or more of the first composition, the second composition, and / or the third composition. In some instances, the kit includes written instructions to perform any one of the methods disclosed herein.

[0033] The terms “a” and “an” are defined as one or more unless this disclosure explicitly requires otherwise. The term “substantially” is defined as being largely but not necessarily wholly what is specified (and include wholly what is specified) as understood by one of ordinary skill in the art. In any disclosed embodiment, the term “substantially” may be substituted with “within [a percentage] of’ what is specified, where the percentage includes 0.1, 1, 5, and 10 percent. The terms “moiety,” “functional group,” “component,” and “compound” may be used interchangeably herein.

[0034] The terms “comprise” (and any form of comprise, such as “comprises” and “comprising”), “have” (and any form of have, such as “has” and “having”), “include” (and any form of include, such as “includes” and “including”) and “contain” (and any form of contain, such as “contains” and “containing”) are open-ended linking verbs. As a result, a method that “comprises,” “has,” “includes” or “contains” one or more steps or elements possesses those one or more steps or elements, but is not limited to possessing only those one or more steps or elements. Likewise, an element of a system or composition that “comprises,” “has,” “includes” or “contains” one or more features possesses those one or more features, but is not limited to possessing only those one or more features.299472458.1 - 15 - OLAP.POOQ9WO / 1001359125

[0035] Furthermore, a structure or composition that is configured in a certain way is configured in at least that way, but may also be configured in ways that are not listed. Metric units may be derived from the English units provided by applying a conversion and rounding to the nearest millimeter.

[0036] The feature or features of one embodiment may be applied to other embodiments, even though not described or illustrated, unless expressly prohibited by this disclosure or the nature of the embodiments. In any of the claims, the term “consisting of’ or “consisting essentially of’ can be substituted for any of the open-ended linking verbs recited above, in order to change the scope of a given claim from what it would otherwise be using the open- ended linking verb.

[0037] It is also contemplated that the compositions disclosed throughout this specification can be used as a leave-on or rinse-off composition. By way of example, a leave-on composition can be one that is topically applied to hair and remains on the hair for a period of time (e.g., at least 5, 6, 7, 8, 9, 10, 20, or 30 minutes, or at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 or 24 hours, or overnight or throughout the day). Alternatively, a rinse-off composition can be a product that is intended to be applied to the hair and then removed or rinsed from the hair (e.g., with water) within a period of time such as less than 5, 4, 3, 2, or 1 minute. An example of a rinse off composition can be a shampoo, conditioner, or soap. An example of a leave-on composition can be a moisturizer, sunscreen, mask, styling gel, serum, leave-in conditioner.

[0038] It is contemplated that any embodiment discussed in this specification can be implemented with respect to any method or composition of the invention, and vice versa. Furthermore, compositions of the invention can be used to achieve methods of the invention.

[0039] In one embodiment, compositions of the present invention can be pharmaceutically or cosmetically elegant or can have pleasant tactile properties. “Pharmaceutically elegant,” “cosmetically elegant,” and / or “pleasant tactile properties” describes a composition that has particular tactile properties which feel pleasant on the skin and / or hair (e.g., compositions that are not too watery or greasy, compositions that have a silky texture, compositions that are non- tacky or sticky, etc.). Pharmaceutically or cosmetically elegant can also relate to the creaminess, weight, or lubricity properties of the composition or to the moisture retaining properties of the composition.

[0040] Also contemplated is a product comprising a composition of the present invention. In non-limiting aspects, the product can be a hair care product. The hair care product can be those described in other sections of this specification or those known to a person of skill in the299472458.1 - 16 - OLAP.POOQ9WO / 1001359125art. Non-limiting examples of products include a hair moisturizer, a hair cream, a hair lotion, a hair softener, a hair gel, a hair wash, a hair cleanser, a hair sunscreen, a hair color-protecting composition, a hair mask, a hair perfume, a hair cologne, a shampoo, a conditioner, etc.

[0041] Also disclosed in the context of the present invention are aspects 1 to 20. Aspect 1 is a method of treating hair, the method comprising applying a composition to the hair to form a treated hair, the composition comprising bis-aminopropyl diglycol dimaleate and guar hydroxypropyltrimonium chloride and one or more of propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum, wherein the treated hair includes at least one of the following features when compared to hair that has not been treated with the composition: (1) increased curl retention; (2) increased strength to reduce breakage of the hair; (3) tensile strength that is not decreased; (4) decreased tangling; and / or (5) reduced hair frizziness and / or hair fly-aways. Aspect 2 is the method of aspect 1, wherein the composition comprises 0.00001 to 1 wt. % of the bis-aminopropyl diglycol dimaleate by weight of the composition. Aspect 3 is the method of any one of aspects 1 to 2, wherein the composition comprises 0.05 to 2 wt. % of the guar hydroxypropyltrimonium chloride by weight of the composition. Aspect 4 is the method of any one of aspects 1 to 3, wherein the composition comprises propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum. Aspect 5 is the method of any one of aspects 1 to 4, wherein the composition comprises, by weight of the composition: 0.1 to 3 wt. % of the propanediol; 0.05 to 2 wt. % of the hydroxypropyl starch phosphate; 0.01 to 1 wt. % of the aspergillus ferment; and / or 0.01 to 1 wt. % of the Acacia senega! gum. Aspect 6 is the method of any one of aspects 1 to 5, wherein the composition further comprises: xylitylglucoside; anhydroxylitol; hydroxyethylcellulose; phenoxyethanol; maltitol; glycerin; caprylyl / capryl glucoside; xylitol; polyimide-1; and / or ethylhexylglycerin. Aspect 7 is the method of aspect 6, wherein the composition comprises, by weight of the composition: 0.1 to 5 wt. % xylitylglucoside; 0.1 to 3 wt. % anhydroxylitol; 0.1 to 3 wt. % hydroxyethylcellulose; 0.1 to 3 wt. % phenoxyethanol; 0.1 to 3 wt. % maltitol; 0.1 to 3 wt. % glycerin; 0.05 to 2 wt. % caprylyl / capryl glucoside; 0.05 to 2 wt. % xylitol; 0.05 to 2 wt. % polyimide-1; and / or 0.01 to 1 wt. % ethylhexylglycerin. Aspect 8 is the method of any one of aspects 1 to 7, wherein the composition remains on the treated hair for at least 2 hours, at least 6 hours, at least 12 hours, or at least 24 hours. Aspect 9 is the method of any one of aspects 1 to 8, wherein the hair prior to applying the composition is curly hair. Aspect 10 is the method of aspect 9, wherein the curly hair is naturally curly hair. Aspect 11 is the method of any one of aspects 1 to 10, wherein the hair prior to applying the composition is color dyed hair and / or bleached hair. Aspect 12 is the method of any one of299472458.1 - 17 - OLAP.POOQ9WO / 1001359125aspects 1 to 11, wherein increased curl retention of the hair comprises maintaining a curled shape of the hair for at least 18 hours, at least 48 hours, or at least 74 hours. Aspect 13 is the method of any one of aspects 1 to 12, wherein the method further comprises styling the hair within 30 minutes, within 1 hour, or within 2 hours of applying the composition to the hair. Aspect 14 is the method of any one of aspects 1 to 13, wherein applying the composition to the hair does not change the color of the hair once the hair is dried as determined by unaided human vision as compared to hair that has not been treated by the composition.

[0042] Aspect 15 is a hair care composition comprising bis-aminopropyl diglycol dimaleate and guar hydroxypropyltrimonium chloride and one or more of propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum. Aspect 16 is the hair care composition of aspect 15, comprising 0.00001 to 1 wt. % of the bis-aminopropyl diglycol dimaleate and / or 0.05 to 2 wt. % of the guar hydroxypropyltrimonium chloride by weight of the composition. Aspect 17 is the hair care composition of any one of aspects 15 to 16, comprising propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum. Aspect 18 is the hair care composition of any one of aspects 15 to 17, comprising, by weight of the composition: 0.1 to 3 wt. % of the propanediol; 0.05 to 2 wt. % of the hydroxypropyl starch phosphate; 0.01 to 1 wt. % of the aspergillus ferment; and / or 0.01 to 1 wt. % of the Acacia senega! gum. Aspect 19 is the hair care composition of any one of aspects 15 to 18, further comprising: xylitylglucoside; anhydroxylitol; hydroxy ethylcellulose; phenoxyethanol; maltitol; glycerin; caprylyl / capryl glucoside; xylitol; polyimide- 1; and / or ethylhexylglycerin. Aspect 20 is the hair care composition of aspect 19, comprising, by weight of the composition: 0.1 to 5 wt. % xylitylglucoside; 0.1 to 3 wt. % anhydroxylitol; 0.1 to 3 wt. % hydroxyethylcellulose; 0.1 to 3 wt. % phenoxyethanol; 0.1 to 3 wt. % maltitol; 0.1 to 3 wt. % glycerin; 0.05 to 2 wt. % caprylyl / capryl glucoside; 0.05 to 2 wt. % xylitol; 0.05 to 2 wt. % polyimide-1; and / or 0.01 to 1 wt. % ethylhexylglycerin.

[0043] Also disclosed in the context of the present invention are aspects 21-40. Aspect 21 is a method of treating hair, the method comprising: (a) applying a first composition to the hair, the first composition comprising PB-300 peptide; (b) applying a second composition to the hair, the second composition comprising hydrogen peroxide, wherein the hydrogen peroxide oxidizes at least a portion of the PB-300 peptide, wherein the hair is at least partially coated with oxidized PB-300 peptide and includes at least one of the following features when compared to hair that has not been subjected to steps (a) and b): (1) increased curl retention; (2) increased strength to reduce breakage of the hair; (3) does not decrease tensile strength; (4) decreased tangling; and / or (5) reduced hair frizziness and / or hair fly-aways. Aspect 22 is the299472458.1 - 18 - OLAP.POOQ9WO / 1001359125method of aspect 21, further comprising applying a third composition to the hair at least partially coated with oxidized PB-300 peptide, the third composition comprising bisaminopropyl diglycol dimaleate. Aspect 23 is the method of aspect 22, wherein the third composition comprises 0.00001 to 1 wt. % of the bis-aminopropyl diglycol dimaleate by weight of the third composition. Aspect 24 is the method of any one of aspects 21 to 23, wherein the first composition comprises 0.00001 to 1 wt. %, by weight of the first composition, of the PB- 300 peptide. Aspect 25 is the method of any one of aspects 21 to 24, wherein the second composition comprises 0.01 to 0.5 wt. %, by weight of the second composition, of the hydrogen peroxide. Aspect 26 is the method of any one of aspects 21 to 25, wherein the first composition further comprises: hydroxyethylcellulose; glycerin; phenoxyethanol; caprylyl glycol; chlorphenesin; and / or trisodium ethylenediamine disuccinate. Aspect 27 is the method of aspect 26, wherein the first composition comprises, by weight of the composition: 0.01 to 1.5 wt. % hydroxyethylcellulose; 0.1 to 5 wt. % glycerin; 0.01 to 1.5 wt. % phenoxyethanol; 0.01 to 1.5 wt. % caprylyl glycol; 0.001 to 1 wt. % chlorphenesin; and / or 0.001 to 1 wt. % trisodium ethylenediamine disuccinate. Aspect 28 is the method of any one of aspects 21 to 27, wherein the second composition further comprises: propanediol; caprylyl / capryl glucoside; and / or sodium cocoyl glutamate. Aspect 29 is the method of aspect 28, wherein the second composition comprises, by weight of the composition: 0.1 to 5 wt. % propanediol; 0.01 to 1.5 wt. % caprylyl / capryl glucoside; and / or 0.001 to 1 wt. % sodium cocoyl glutamate. Aspect 30 is the method of any one of aspects 22 to 29, wherein the third composition further comprises: xylitylglucoside; anhydroxylitol; hydroxyethylcellulose; phenoxyethanol; maltitol; glycerin; caprylyl / capryl glucoside; xylitol; sodium polyacryloyldimethyl taurate; PVM / MA copolymer; ethylhexylglycerin; and / or polyimide-1. Aspect 31 is the method of aspect 10, wherein the third composition comprises, by weight of the composition: 0.1 to 5 wt. % xylitylglucoside; 0.1 to 5 wt. % anhydroxylitol; 0.1 to 5 wt. % hydroxyethylcellulose; 0.1 to 5 wt. % phenoxyethanol; 0.01 to 1.5 wt. % maltitol; 0.01 to 1.5 wt. % glycerin; 0.01 to 1.5 wt. % caprylyl / capryl glucoside; 0.01 to 1.5 wt. % xylitol; 0.01 to 1.5 wt. % sodium polyacryloyldimethyl taurate; 0.01 to 1.5 wt. % PVM / MA copolymer; 0.001 to 1 wt. % ethylhexylglycerin; and / or 0.001 to 1 wt. % polyimide-1. Aspect 32 is the method of any one of aspects 21 to 31, wherein the second composition is applied to the hair within 1 hour, within 30 minutes, or within 20 minutes of the first composition being applied to the hair. Aspect 33 is the method of any one of aspects 22 to 32, wherein the third composition is applied to the hair at least partially coated with oxidized PB-300 peptide within 1 hour, within 30 minutes, or within 20 minutes of the second composition being applied to the hair. Aspect 34 is the method299472458.1 - 19 - OLAP.POOQ9WO / 1001359125of any one of aspects 22 to 33, wherein the third composition remains on the hair for at least 2 hours, at least 6 hours, at least 12 hours, or at least 24 hours. Aspect 35 is the method of any one of aspects 21 to 34, wherein the hair prior to steps (a) and (b) is curly hair. Aspect 36 is the method of aspect 35, wherein the curly hair is naturally curly hair. Aspect 37 is the method of any one of aspects 21 to 36, wherein the hair prior to steps (a) and (b) is color dyed hair and / or bleached hair. Aspect 38 is the method of any one of aspects 21 to 37, wherein increased curl retention of the hair comprises maintaining a curled shape of the hair for at least 18 hours, at least 48 hours, or at least 74 hours. Aspect 39 is the method of any one of aspects 21 to 38, wherein the method further comprises styling the hair within 30 minutes, within 1 hour, or within 2 hours of applying the second composition and / or the third composition to the hair. Aspect for is the method of any one of aspects 21 to 39, wherein steps (a) and (b) do not change the color of the hair once the hair is dried as determined by unaided human vision as compared to hair that has not been subjected to steps (a) and (b).

[0044] The terms “wt.%,” “% by weight,” “vol.%,” “% by volume,” “mol.%,” or “% by mol.” refers to a weight percentage of a component, a volume percentage of a component, or molar percentage of a component, respectively, based on the total weight, the total volume of material, or total moles, which includes the component. In a non-limiting example, 10 grams of component in 100 grams of the material is 10 wt.% of component.

[0045] A hair care composition as used herein may refer to a product that can be used to clean, treat or style hair. Examples of hair care compositions include, but are not limited to shampoos, conditioners, styling gels, aerosol styling sprays, non-aerosol styling sprays, aerosol styling mousses, styling gels, styling pomades, leave-in conditioning sprays, and thermal protection sprays.

[0046] The term “about” or “approximately” are defined as being close to as understood by one of ordinary skill in the art. In one non-limiting embodiment the terms are defined to be within 10%, preferably within 5%, more preferably within 1%, and most preferably within 0.5%.

[0047] The term “substantially” and its variations are refers to ranges within 10%, within 5%, within 1%, or within 0.5%.

[0048] The terms “inhibiting” or “reducing” or any variation of these terms includes any measurable decrease or complete inhibition to achieve a desired result. The terms “promote” or “increase” or any variation of these terms includes any measurable increase to achieve a desired result.299472458.1 - 20 - OLAP.POOQ9WO / 1001359125

[0049] The term “effective,” as that term is used in the specification and / or claims, means adequate to accomplish a desired, expected, or intended result.

[0050] The use of the word “a” or “an” when used in conjunction with the terms “comprising,” “including,” “having,” or “containing,” or any variations of these terms, in the claims and / or the specification may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.”

[0051] The compositions and methods for their use can “comprise,” “consist essentially of,” or “consist of’ any of the ingredients or steps disclosed throughout the specification. With respect to the phrase “consisting essentially of,” a basic and novel property of the compositions and methods of the present invention is the ability to treat hair ((1) increase curl retention in hair; (2) increase strength of the hair to reduce breakage of the hair; (3) not negatively effect the hair shafts tensile strength; (4) decrease hair tangling; and / or (5) reduce the appearance of hair frizziness and / or hair fly-aways).

[0052] The term “hair” refers to one or more than one strand of hair. Hair also refers to virgin hair or processed hair, for example hair that has been exposed to hair waving, color treatment, and / or hair straightening formulations.

[0053] “Shampoo,” as used herein, generally refers to a liquid or semi-solid formulation applied to the hair that contains detergent or soap for washing the hair.

[0054] Conditioner,” as used herein, generally refers to a formulation (e.g., liquid, cream, lotion, gel, semi-solid) applied to the hair to soften the hair, smooth the hair, and / or change the sheen of the hair.

[0055] “Analog” and “Derivative” are used herein interchangeably and refer to a compound that possesses the same core as the parent compound, but differs from the parent compound in bond order, the absence or presence of one or more atoms and / or groups of atoms, and combinations thereof. The derivative can differ from the parent compound, for example, in one or more substituents present on the core, which may include one or more atoms, functional groups, or substructures. In general, a derivative can be imagined to be formed, at least theoretically, from the parent compound via chemical and / or physical processes.

[0056] “Alkyl,” as used herein, refers to the radical of saturated or unsaturated aliphatic groups, including straight-chain alkyl, alkenyl, or alkynyl groups, branched-chain alkyl, alkenyl, or alkynyl groups, cycloalkyl, cycloalkenyl, or cycloalkynyl (alicyclic) groups, alkyl substituted cycloalkyl, cycloalkenyl, or cycloalkynyl groups, and cycloalkyl substituted alkyl, alkenyl, or alkynyl groups. Unless otherwise indicated, a straight chain or branched chain alkyl has 30 or fewer carbon atoms in its backbone (e.g., C1-C30 for straight chain, C3-C30 for299472458.1 - 21 - OLAP.POOQ9WO / 1001359125branched chain), more preferably 20 or fewer carbon atoms, more preferably 12 or fewer carbon atoms, and most preferably 8 or fewer carbon atoms. In some embodiments, the chain has 1-6 carbons. Likewise, preferred cycloalkyls have from 3-10 carbon atoms in their ring structure, and more preferably have 5, 6 or 7 carbons in the ring structure. The ranges provided above are inclusive of all values between the minimum value and the maximum value.

[0057] The term “alkyl” includes both “unsubstituted alkyls” and “substituted alkyls,” the latter of which refers to alkyl moieties having one or more substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents include, but are not limited to, halogen, hydroxyl, carbonyl (such as a carboxyl, alkoxycarbonyl, formyl, or an acyl), thiocarbonyl (such as a thioester, a thioacetate, or a thioformate), alkoxyl, phosphoryl, phosphate, phosphonate, a phosphinate, amino, amido, amidine, imine, cyano, nitro, azido, sulfhydryl, alkylthio, sulfate, sulfonate, sulfamoyl, sulfonamido, sulfonyl, heterocyclyl, aralkyl, or an aromatic or heteroaromatic moiety.

[0058] The alkyl groups may also contain one or more heteroatoms within the carbon backbone. Examples include oxygen, nitrogen, sulfur, and combinations thereof. In certain embodiments, the alkyl group contains between one and four heteroatoms.

[0059] “Alkenyl” and “Alkynyl,” as used herein, refer to unsaturated aliphatic groups containing one or more double or triple bonds analogous in length (e.g., C2-C30) and possible substitution to the alkyl groups described above.

[0060] “Aryl,” as used herein, refers to 5-, 6- and 7-membered aromatic rings. The ring may be a carbocyclic, heterocyclic, fused carbocyclic, fused heterocyclic, bicarbocyclic, or biheterocyclic ring system, optionally substituted as described above for alkyl. Broadly defined, “Ar,” as used herein, includes 5-, 6- and 7-membered single-ring aromatic groups that may include from zero to four heteroatoms. Examples include, but are not limited to, benzene, pyrrole, furan, thiophene, imidazole, oxazole, thiazole, triazole, pyrazole, pyridine, pyrazine, pyridazine and pyrimidine. Those aryl groups having heteroatoms in the ring structure may also be referred to as “heteroaryl,” “aryl heterocycles,” or “heteroaromatics.” The aromatic ring can be substituted at one or more ring positions with such substituents as described above, for example, halogen, azide, alkyl, aralkyl, alkenyl, alkynyl, cycloalkyl, hydroxyl, alkoxyl, amino, nitro, sulfhydryl, imino, amido, phosphonate, phosphinate, carbonyl, carboxyl, silyl, ether, alkylthio, sulfonyl, sulfonamido, ketone, aldehyde, ester, heterocyclyl, aromatic or heteroaromatic moieties, perfluoroalkyl, and cyano. The term “Ar” also includes polycyclic ring systems having two or more cyclic rings in which two or more carbons are common to two adjoining rings (the rings are “fused rings”) wherein at least one of the rings is aromatic,299472458.1 - 22 - OLAP.POOQ9WO / 1001359125e.g., the other cyclic rings can be cycloalkyls, cycloalkenyls, cycloalkynyls, aryls and / or heterocycles, or both rings are aromatic.

[0061] “Heterocycle” or “heterocyclic,” as used herein, refers to a cyclic radical attached via a ring carbon or nitrogen of a monocyclic or bicyclic ring containing 3-10 ring atoms, and preferably from 5-6 ring atoms, containing carbon and one to four heteroatoms each selected from non-peroxide oxygen, sulfur, and N(Y) wherein Y is absent or is H, O, (Cl -4) alkyl, phenyl or benzyl, and optionally containing one or more double or triple bonds, and optionally substituted with one or more substituents. The term “heterocycle” also encompasses substituted and unsubstituted heteroaryl rings. Examples of heterocyclic ring include, but are not limited to, benzimidazolyl, benzofuranyl, benzothiofuranyl, benzothiophenyl, benzoxazolyl, benzoxazolinyl, benzthiazolyl, benztriazolyl, benztetrazolyl, benzisoxazolyl, benzisothiazolyl, benzimidazolinyl, carbazolyl, 4aH-carbazolyl, carbolinyl, chromanyl, chromenyl, cinnolinyl, decahydroquinolinyl, 2H,6H-l,5,2-dithiazinyl, dihydrofuro[2,3-b]tetrahydrofuranyl, furanyl, furazanyl, imidazolidinyl, imidazolinyl, imidazolyl, IH-indazolyl, indolenyl, indolinyl, indolizinyl, indolyl, 3H-indolyl, isatinoyl, isobenzofuranyl, isochromanyl, isoindazolyl, isoindolinyl, isoindolyl, isoquinolinyl, isothiazolyl, isoxazolyl, methylenedi oxy phenyl, morpholinyl, naphthyridinyl, octahydroisoquinolinyl, oxadiazolyl, 1,2,3-oxadiazolyl, 1,2,4- oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, oxazolidinyl, oxazolyl, oxindolyl, pyrimidinyl, phenanthridinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, phenoxathinyl, phenoxazinyl, phthalazinyl, piperazinyl, piperidinyl, piperidonyl, 4-piperidonyl, piperonyl, pteridinyl, purinyl, pyranyl, pyrazinyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridooxazole, pyridoimidazole, pyridothiazole, pyridinyl, pyridyl, pyrimidinyl, pyrrolidinyl, pyrrolinyl, 2H-pyrrolyl, pyrrolyl, quinazolinyl, quinolinyl, 4H-quinolizinyl, quinoxalinyl, quinuclidinyl, tetrahydrofuranyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl, tetrazolyl, 6H- 1,2,5-thiadiazinyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, thianthrenyl, thiazolyl, thienyl, thi enothiazolyl, thienooxazolyl, thienoimidazolyl, thiophenyl and xanthenyl.

[0062] “Heteroaryl,” as used herein, refers to a monocyclic aromatic ring containing five or six ring atoms containing carbon and 1, 2, 3, or 4 heteroatoms each selected from nonperoxide oxygen, sulfur, and N(Y) where Y is absent or is H, O, (C1-C8) alkyl, phenyl or benzyl. Non-limiting examples of heteroaryl groups include furyl, imidazolyl, triazolyl, triazinyl, oxazoyl, isoxazoyl, thiazolyl, isothiazoyl, pyrazolyl, pyrrolyl, pyrazinyl, tetrazolyl, pyridyl, (or its N-oxide), thienyl, pyrimidinyl (or its N-oxide), indolyl, isoquinolyl (or its N- oxide), quinolyl (or its N-oxide) and the like. The term “heteroaryl” can include radicals of an299472458.1 - 23 - OLAP.POOQ9WO / 1001359125ortho-fused bicyclic heterocycle of about eight to ten ring atoms derived therefrom, particularly a benzyl-derivative or one derived by fusing a propylene, trimethylene, or tetramethylene diradical thereto. Examples of heteroaryl include, but are not limited to, furyl, imidazolyl, triazolyl, triazinyl, oxazoyl, isoxazoyl, thiazolyl, isothiazoyl, pyrazolyl, pyrrolyl, pyrazinyl, tetrazolyl, pyridyl (or its N-oxide), thienyl, pyrimidinyl (or its N-oxide), indolyl, isoquinolyl (or its N-oxide), quinolyl (or its N-oxide), and the like.

[0063] “Halogen,” as used herein, refers to fluorine, chlorine, bromine, or iodine.

[0064] The term “substituted” as used herein, refers to all permissible substituents of the compounds described herein. In the broadest sense, the permissible substituents include acyclic and cyclic, branched and unbranched, carbocyclic and heterocyclic, aromatic and nonaromatic substituents of organic compounds. Illustrative substituents include, but are not limited to, halogens, hydroxyl groups, or any other organic groupings containing any number of carbon atoms, preferably 1-14 carbon atoms, and optionally include one or more heteroatoms such as oxygen, sulfur, or nitrogen grouping in linear, branched, or cyclic structural formats. Representative substituents include alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, phenyl, substituted phenyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, halo, hydroxyl, alkoxy, substituted alkoxy, phenoxy, substituted phenoxy, aroxy, substituted aroxy, alkylthio, substituted alkylthio, phenylthio, substituted phenylthio, arylthio, substituted arylthio, cyano, isocyano, substituted isocyano, carbonyl, substituted carbonyl, carboxyl, substituted carboxyl, amino, substituted amino, amido, substituted amido, sulfonyl, substituted sulfonyl, sulfonic acid, phosphoryl, substituted phosphoryl, phosphonyl, substituted phosphonyl, polyaryl, substituted polyaryl, C3-C20 cyclic, substituted C3-C20 cyclic, heterocyclic, substituted heterocyclic, amino acid, peptide, and polypeptide groups.

[0065] Heteroatoms, such as nitrogen, may have hydrogen substituents and / or any permissible substituents of organic compounds described herein that satisfy the valences of the heteroatoms. It is understood that “substitution” or “substituted” includes the implicit proviso that such substitution is in accordance with permitted valence of the substituted atom and the substituent, and that the substitution results in a stable compound, i.e. a compound that does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, etc.

[0066] The term “linker,” as used herein, refers to one or more polyfunctional, e.g. bifunctional molecules, trifunctional molecules, tetrafunctional molecules, etc., which can be used to ionically bound the two or more moieties and which do not interfere with the reactive299472458.1 - 24 - OLAP.POOQ9WO / 1001359125properties of the moieties. The moieties may be attached to any part of the linker. Linkers can be a single atom, such as a heteroatom (e.g., O or S), a group of atoms, such as a functional group (e.g., amine, — C(=O) — , — CH2 — ), or multiple groups of atoms, such as an alkylene chain. Suitable linkers include but are not limited to oxygen, sulfur, carbon, boron, nitrogen, alkoxy, alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heterocycloalkyl, heteroaryl, ether, amine, and a polymer. The linker is optionally independently substituted with one or more substituents including hydrogen, halogen, cyano, alkoxy, alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heterocycloalkyl, heteroaryl, amine, hydroxy, formyl, acyl, carboxylic acid ( — COOH), — C(O)R1, — C(O)OR1, carboxylate ( — COO-), primary amide (e.g., — C0NH2), secondary amide (e g., — C0NHR1), — C(O)NR1R2, — NR1R2, — NR1S(O)2R2, — NR1C(O)R2, — S(O)2R2, —SRI, and — S(O)2NR1R2, sulfinyl group (e.g., —SORI), and sulfonyl group (e.g., — SOOR1); wherein R1 and R2 may each independently be hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heterocycloalkyl and heteroaryl; wherein each of R1 and R2 is optionally independently substituted with one or more substituents selected from the group consisting of halogen, hydroxyl, cyano, nitro, amino, alkylamino, dialkylamino, alkyl optionally substituted with one or more halogen or alkoxy or aryloxy, aryl optionally substituted with one or more halogen or alkoxy or alkyl or trihaloalkyl, heterocycloalkyl optionally substituted with aryl or heteroaryl or =0 or alkyl optionally substituted with hydroxyl, cycloalkyl optionally substituted with hydroxyl, heteroaryl optionally substituted with one or more halogen or alkoxy or alkyl or trihaloalkyl, haloalkyl, hydroxyalkyl, carboxy, alkoxy, aryloxy, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl and dialkylaminocarbonyl. In some embodiments, the linker may be an alkoxy, ether, alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, heterocycloalkyl, heteroaryl, amine, or a polymer. In some embodiments, the linker is not a polymer.

[0067] “Reactive moieties” are capable of reacting with a thiol group in the hair or on the skin to form a stable covalent bond. The reactive moiety is typically an electrophilic moiety capable of forming a salt with the linker. Alternately, the reactive moiety can be a free radical forming moiety. The reaction between the reactive moiety and the thiol groups may be initiated at room temperature and pressure when the reactive moiety contacts a thiol group in the hair or on the skin. In some embodiments, the reaction may require an initiator, such as heat, catalyst, basic conditions, or a free radical initiator. The rate of reaction between the reactive moiety and the thiol may be increased by changes in temperature, pH, and / or addition of one or more excipients, such as a catalyst; however, this is generally not required. In some embodiments, the reactive moieties are capable of undergoing a conjugate additional reaction.299472458.1 - 25 - OLAP.POOQ9WO / 1001359125The reactive moieties can independently be or contain a Michael acceptor, a succinimidyl- containing group, a maleimido-containing group, azlactone, a benzoxazinone derivative, vinyl sulfone, vinyl sulfoximine, vinyl sulfonate, vinyl phosphonate, benzoxazinone, isocyanate, epoxide, an electrophilic moiety containing a leaving group, an electrophilic thiol acceptor, acrylic or acrylate group, a methacrylic or methacrylate group, a styrene group, an acryl amide group, a methacryl amide group, a maleate group, a fumarate group, an itaconate group, a vinyl ether group, an allyl ether group, an allyl ester group, a vinyl ester group, a sulfonate group, a phosphonate group, a sulfoxide group, a sulfonamide group, a sulfmimide group, a sulfmamide group, a sulfonimidate group, or a sulfonimidamide group. Examples of suitable reactive moieties and compounds of Formula I can be found in United States Patent Application Publication No. 2023 / 0201632A1, incorporated herein in its entirety.

[0068] Soluble” in a solvent, such as water, as used herein, generally means at least 10, 50, 100, 125, 150, 200, 225, or 250 g is soluble in 1 L of the solvent at 25° C.

[0069] Other objects, features, and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and the examples, while indicating specific embodiments of the invention, are given by way of illustration only. Additionally, it is contemplated that changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.DETAILED DESCRIPTION

[0066] As noted above, methods and compositions for treating hair to strengthen hair and / or retain and / or restore shape of the hair are disclosed herein. The methods and compositions use PB-300 peptide, hydrogen peroxide, and / or bis-aminopropyl diglycol dimaleate. The methods may use multiple steps. In some instances, the steps include applying a composition containing PB-300 peptide to hair, applying a composition containing hydrogen peroxide to oxidize the PB-300 peptide, and / or applying a composition containing bis- aminopropyl diglycol dimaleate to the hair. The methods and compositions may increase curl retention, increase strength to reduce breakage of the hair, decrease tangling, and / or reduce hair frizziness and / or hair fly-aways, without decreasing hair tensile strength.

[0067] These and other non-limiting aspects of the present invention are provided below.A. Active Agents

[0068] PB-300 peptide is a peptide containing twenty three amino acids, including six cysteines, having an amino acid sequence Cys-Gly-Gln-Val-Cys-His-Leu-Gln-Cys-Ala-Phe-299472458.1 - 26 - OLAP.POOQ9WO / 1001359125Ser-Cys-Tyr-Lys-Lys-Cys-Glu-Leu-Phe-Pro-Lys-Cys (SEQ ID NO: 1). Applicant has determined that PB-300 peptide can be used on hair to improve the condition of the hair. Applicant has found that oxidation of PB-300 peptide improves the activity of the peptide to improve the condition of the hair. PB-300 peptide may be used on the hair or in a composition in concentrations such as equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, or 1 wt. %.

[0069] In some instances, the first composition contains along with the PB-300 peptide or instead of the PB-300 peptide a peptide described in U.S. Patent Number 11,596,589, titled “Compositions and Method of Use for Hair Straightening and Shaping,” which patent is hereby incorporated by reference and / or a peptide with a sequences having at least one amino acid difference from SEQ ID NO: 1 and typically capable of binding to keratin. In some instances, any one or more of the amino acids in the amino acid sequence of SEQ ID NO: 1, can be replaced or substituted with cysteine.

[0070] Hydrogen peroxide is an oxidant that can be used on hair and has been used on hair to lighten hair. However, high amounts and / or multiple exposures to hair can cause damage to hair. In some instances, the amount of hydrogen peroxide used in the method or included in a composition herein is less than the amount typically used to lighten hair, bleach hair, straighten curly hair, or curl straight hair. The amount of hydrogen peroxide used can be in some instances less than 2.5, less than 2, less than 1.5, less than 1, or less than 0.5 wt. % by weight of the composition, such as equal to, more than, less than, a range of, or between 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, or 2.5 wt. %. In some instances, the hydrogen peroxide is stabilized in the composition, such as by adjusting the pH of the composition to increase shelf life of the hydrogen peroxide. Hydrogen peroxide is available from commercial sources. In some instances, the hydrogen peroxide used or present in the composition(s) is from a commercial source.299472458.1 - 27 - OLAP.POOQ9WO / 1001359125

[0071] Bis-aminopropyl diglycol dimaleate is a compound that can be used on hair and has been used on hair to restore and / or provide sulfur bridges of hair. In some instances, the compositions and / or methods herein replace the bis-aminopropyl diglycol dimaleate with, or use in addition to the bis-aminopropyl diglycol dimaleate, one or more of the compounds of Formula I that can restore and / or provide sulfur bridges of hair. In some instances, the concentration of the bis-aminopropyl diglycol dimaleate and / or one or more of the compounds of Formula I is individually equal to, more than, less than, a range of, or between 0.00001, 0.00005, 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44,0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61,0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95,0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 wt. %

[0072] One or more additional active agents may be added to the haircare compositions. The combination of the active agents may have additive or synergistic effects.B. Additives

[0073] The hair compositions may include one or more of each of water, a sunscreen, a non-ionic, anionic, cationic, or zwitterionic surfactant, vitamins, pH adjusters, fragrance, solvents, chelating agents, preservatives, opacifying agents, volatiles, propellants, liquid vehicles, carriers, salts, buffers, emulsifying agents, softeners, fillers, moisturizers, hair fixatives, dyes, colorants, pigments, antifoaming agents, antioxidants, moisturizing agents, proteins, peptides, amino acids, extracts, resins, essential oils, thickening agents, viscosity modifiers, film formers, emollients, lubricants, butters, penetrants, anti-static agents, anti-frizz agents, anti-dandruff agents, absorbents, surfactants, detergents, pharmaceutically-active agents, vitamins, and other additives used by those skilled in the art.C. Surfactants

[0074] Surfactants may be amphoteric, anionic, or cationic. Exemplary surfactants include but are not limited to cocamidopropyl betaine, coco betaine, lauryl betaine, oleyl betaine, stearyl betaine, coco sultaine, lauryl sultaine, sodium cocoyl isethionate, sodium methyl 2- sulfolaurate, disodium 2-sulfolaurate, sodium lauryl sulfoacetate, ammonium lauryl sulfate, ammonium laureth sulfate, sodium lauryl sulfate, sodium laureth sulfate, sodium lauroyl299472458.1 - 28 - OLAP.POOQ9WO / 1001359125sarcosinate, cocoyl sarcosine, sodium cocoyl lactylate, sodium cocoyl sarcosinate, 3- aminopropane sulfonic acid, almond amide, almond amidopropyl betaine, almond amidopropylamine oxide, aluminum hydrogenated tallow glutamate, aluminum lanolate, aminoethyl sulfate, aminopropyl lauryl glutamine, ammonium C 12-15 alkyl sulfate, ammonium C12-15 pareth sulfate, ammonium C12-16 alkyl sulfate, ammonium C9-10 perfluoroalkyl sulfonate, ammonium capryleth sulfate, ammonium capryleth-3 sulfate, ammonium monoglyceride sulfate, ammonium sulfate, ammonium isothionate, ammonium cocoyl sarcosinate, ammonium cumene sulfonate, ammonium dimethicone copolyol sulfate, ammonium dodecylbenzenesulfonate, ammonium isostearate, ammonium laureth-12 sulfate, ammonium laureth-5 sulfate, ammonium laureth-6 carboxylate, ammonium laureth-7 sulfate, ammonium laureth-8 carboxylate, ammonium laureth-9 sulfate, ammonium lauroyl sarcosinate, ammonium lauryl sulfosuccinate, ammonium myreth sulfate, ammonium myristyl sulfate, ammonium nonoxynol-30 sulfate, ammonium nonoxynol-4 sulfate, ammonium oleate, ammonium palm kernel sulfate, ammonium polyacrylate, ammonium stearate, ammonium tallate, ammonium xylene sulfonate, ammonium xylene sulfonate, amp-isostearoyl gelatin / keratin amino acids / lysine hydroxypropyltrimonium chloride, amp-isostearoyl hydrolyzed collagen, apricot kernel oil PEG-6 esters, apricot amide, apricot amidopropyl betaine, arachideth-20, avocadamide, avocadamidopropyl betaine, babassuamide, babassuamidopropyl betaine, babassuamidopropylamine oxide, behenalkonium chloride, behenamide, behenamide, behenamidopropyl betaine, behenamine oxide, a polyoxyether of lauryl alcohol or ceteareth-20, or combinations thereof.

[0075] Suitable anionic surfactants include, but are not limited to, those containing carboxylate, sulfonate and sulfate ions. Examples of anionic surfactants include sodium, potassium, ammonium of long chain alkyl sulfonates and alkyl aryl sulfonates such as sodium dodecylbenzene sulfonate; dialkyl sodium sulfosuccinates, such as sodium dodecylbenzene sulfonate; dialkyl sodium sulfosuccinates, such as sodium bis-(2-ethylthioxyl)-sulfosuccinate; and alkyl sulfates such as sodium lauryl sulfate. Cationic surfactants include, but are not limited to, quaternary ammonium compounds such as benzalkonium chloride, benzethonium chloride, cetrimonium bromide, stearyl dimethylbenzyl ammonium chloride, polyoxyethylene and coconut amine. Examples of nonionic surfactants include ethylene glycol monostearate, propylene glycol myristate, glyceryl monostearate, glyceryl stearate, polyglyceryl-4-oleate, sorbitan acylate, sucrose acylate, PEG-150 laurate, PEG-400 monolaurate, polyoxyethylene monolaurate, polysorbates, polyoxyethylene octylphenylether, PEG- 1000 cetyl ether, polyoxyethylene tridecyl ether, polypropylene glycol butyl ether, Pol oxamer 401, stearoyl299472458.1 - 29 - OLAP.POOQ9WO / 1001359125monoisopropanolamide, and polyoxyethylene hydrogenated tallow amide. Examples of amphoteric surfactants include sodium N-dodecyl-.beta.-alanine, sodium N-lauryl-P- iminodipropionate, myristoamphoacetate, lauryl betaine and lauryl sulfobetaine.

[0076] The surfactants are optionally included in an amount ranging from about 0.001% to about 20% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 wt. %.D. Vitamins

[0077] Non-limiting examples of vitamins that may be added to the present compositions include niacinamide, sodium starch octenyl succinate, calcium pantothenate, maltodextrin, sodium ascorbyl phosphate, pyridoxine HC1, silica, panthenol, phytantriol, calcium pantothenate, vitamin E, and vitamin E esters (e. g. , tocopheryl acetate, tocopheryl nocotinate, tocopheryl palmitate, or tocopheryl retinoate).E. Essential Oils

[0078] Essential oils include oils derived from herbs, flowers, trees, and other plants. Such oils are typically present as tiny droplets between the plant’s cells, and can be extracted by several method known to those of skill in the art (e.g., steam distilled, enfleurage (i.e., extraction by using fat), maceration, solvent extraction, or mechanical pressing). When these types of oils are exposed to air they tend to evaporate (i.e., a volatile oil). As a result, many essential oils are colorless, but with age they can oxidize and become darker. Essential oils are insoluble in water and are soluble in alcohol, ether, fixed oils (vegetal), and other organic solvents. Typical physical characteristics found in essential oils include boiling points that vary from about 160° to 240° C and densities ranging from about 0.759 to about 1.096.

[0079] Essential oils typically are named by the plant from which the oil is found. For example, rose oil or peppermint oil are derived from rose or peppermint plants, respectively. Non-limiting examples of essential oils that can be used in the context of the present invention include sesame oil, macadamia nut oil, tea tree oil, evening primrose oil, Spanish sage oil, Spanish rosemary oil, coriander oil, thyme oil, pimento berries oil, rose oil, anise oil, balsam299472458.1 - 30 - OLAP.POOQ9WO / 1001359125oil, bergamot oil, rosewood oil, cedar oil, chamomile oil, sage oil, clary sage oil, clove oil, cypress oil, eucalyptus oil, fennel oil, sea fennel oil, frankincense oil, geranium oil, ginger oil, grapefruit oil, jasmine oil, juniper oil, lavender oil, lemon oil, lemongrass oil, lime oil, mandarin oil, marjoram oil, myrrh oil, neroli oil, orange oil, patchouli oil, pepper oil, black pepper oil, petitgrain oil, pine oil, rose otto oil, rosemary oil, sandalwood oil, spearmint oil, spikenard oil, vetiver oil, wintergreen oil, or ylang ylang. Other essential oils known to those of skill in the art are also contemplated as being useful within the context of the present invention..F. pH Adjusters

[0080] Non-limiting examples of pH adjusters that may be used with the present compositions include common acids and bases, including but not limited to citric acid, acetic acid, benzoic acid, glycolic acid, lactic acid, malic acid, potassium hydroxide, potassium carbonate, sodium carbonate, sodium hydroxide, ethanolamine, and triethanolamine.G. Solvents

[0081] Non-limiting examples of solvents that may be included in the present ultraviolet light-absorbing compositions include but are not limited to water, propylene glycol, propane diol, glycerol, SD alcohol, mineral oil, butanediol, cyclomethicone, and ethoxy glycol.H. Preservatives

[0082] Non-limiting examples of preservatives include but are not limited to benzoic acid, benzoic acid salts, benzyl alcohol, butylparaben, propylparaben, methyparaben, isobutylparaben, methylisothiazolinone, methylchloroisothiazolinone, phenoxy-ethanol, quaterium-8, quaterium-14, quaterium-15, triclosan, zinc pyrithione, zinc salicylate, quaternary ammonium preservatives such as polyquatemium-1 and benzalkonium halides (e.g., benzalkonium chloride (“BAC”) and benzalkonium bromide), parabens (e.g., methylparabens and propylparabens), phenoxyethanol, benzyl alcohol, chlorobutanol, phenol, sorbic acid, thimerosal, glycerin containing compounds (e.g., glycerin or ethylhexylglycerin or phenoxyethanol), sodium benzoate, ethylenediamine-tetraacetic acid (EDTA), potassium sorbate, grapefruit seed extract, salicylic acid, DMDM hydantoin, formaldahyde, chlorphenism, triclosan, imidazolidinyl urea, diazolidinyl urea, sodium dehydroacetate, dehydroacetic acid, quaternium-15, stearalkonium chloride, sodium metabisulfite, 2-bromo-2- nitropropane, chlorhexidine digluconate, polyaminopropyl biguanide, benzalkonium chloride, sodium sulfite, sodium salicylate, citric acid, neem oil, essential oils (various), lactic acid, and vitamin E (tocopherol), or combinations thereof.299472458.1 - 31 - OLAP.POOQ9WO / 1001359125

[0083] The preservatives are optionally included in an amount ranging from about 0.001% to about 7% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, or 7 wt. %.I. Emulsifying Agents

[0084] Non-limiting examples of emulsifying agents that can be used with the present compositions include nonionic, cationic, anionic, and zwitterionic emulsifiers (See McCutcheon’s (1986); U.S. Pat. Nos. 5,011,681; 4,421,769; 3,755,560). Non-limiting examples include esters of glycerin, esters of propylene glycol, fatty acid esters of polyethylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, esters of sorbitan anhydrides, carboxylic acid copolymers, esters and ethers of glucose, copolymers of an unsaturated ester and styrene sulfonate monomer, ethoxylated ethers, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates such as polyoxyethylene glycol ether of cetearyl alcohol, fatty acid amides, acyl lactylates, soaps, TEA stearate, DEA oleth-3 phosphate, polyethylene glycol 20 sorbitan monolaurate (polysorbate 20), polyethylene glycol 5 soya sterol, steareth-2, steareth-20, steareth-21, stearic acid, ceteareth-20, cetearyl glucoside, cetearyl alcohol, C12-13 pareth-3, PPG-2 methyl glucose ether distearate, PPG-5-ceteth-20, bis-PEG / PPG-20 / 20 dimethicone, ceteth-10, polysorbate 80, cetyl phosphate, potassium cetyl phosphate, diethanolamine cetyl phosphate, polysorbate 60, glyceryl stearate, PEG- 100 stearate, arachidyl alcohol, arachidyl glucoside, lecithin, glycol stearate, and mixtures thereof.

[0085] The emulsifying agents are optionally included in an amount ranging from about 0.001% to about 20% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79,299472458.1 - 32 - OLAP.POOQ9WO / 10013591250.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 wt. %.J. Antioxidants

[0086] Non-limiting examples of antioxidants that can be used with the compositions of the present invention include acetyl cysteine, ubiquinone, ascorbic acid polypeptide, ascorbyl dipalmitate, ascorbyl methylsilanol pectinate, ascorbyl palmitate, ascorbyl stearate, BHA, BHT, t-butyl hydroquinone, zinc, cysteine, cysteine HCI, diamylhydroquinone, di-t- butylhydroquinone, dicetyl thiodipropionate, dioleyl tocopheryl methylsilanol, lycopene, disodium ascorbyl sulfate, distearyl thiodipropionate, ditridecyl thiodipropionate, dodecyl gallate, erythorbic acid, esters of ascorbic acid, ethyl ferulate, ferulic acid, gallic acid esters, hydroquinone, isooctyl thioglycolate, kojic acid, magnesium ascorbate, magnesium ascorbyl phosphate, methylsilanol ascorbate, natural botanical anti-oxidants such as green tea or grape seed extracts, arbutin, nordihydroguaiaretic acid, octyl gallate, phenylthioglycolic acid, resveratrol, potassium ascorbyl tocopheryl phosphate, tetrahexydecyl ascorbate, potassium sulfite, propyl gallate, quinones, rosmarinic acid, sodium ascorbate, sodium bisulfite, sodium erythorbate, sodium metabisulfite, sodium sulfite, superoxide dismutase, carotenoids, sodium thioglycolate, sorbityl furfural, thiodiglycol, thiodiglycolamide, thiodiglycolic acid, thioglycolic acid, thiolactic acid, thiosalicylic acid, triethyl citrate, tocophereth-5, tocophereth- 10, tocophereth-12, tocophereth-18, tocophereth-50, tocopherol, tocophersolan, tocopheryl acetate, tocopheryl linoleate, tocopheryl nicotinate, tocopheryl succinate, and tris(nonylphenyl)phosphite.

[0087] The antioxidant agents are optionally included in an amount ranging from about 0.001% to about 20% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 wt. %.K. Moisturizing Agents

[0088] Non-limiting examples of moisturizing agents that can be used with the compositions of the present invention include amino acids, chondroitin sulfate, diglycerin,299472458.1 - 33 - OLAP.POOQ9WO / 1001359125erythritol, fructose, glucose, glycerin, glycerol polymers, glycol, 1,2,6-hexanetriol, honey, hyaluronic acid, hydrogenated honey, hydrogenated starch hydrolysate, inositol, lactitol, maltitol, maltose, mannitol, natural moisturizing factor, PEG- 15 butanediol, polyglyceryl sorbitol, salts of pyrrolidone carboxylic acid, potassium PCA, propylene glycol, saccharide isomerate, sodium glucuronate, sodium PCA, sorbitol, sucrose, trehalose, urea, and xylitol.

[0089] Other examples include acetylated lanolin, acetylated lanolin alcohol, alanine, algae extract, Aloe barbadensis. Aloe barbadensis extract, Aloe barbadensis gel, Althea officinalis extract, apricot (Primus armeniaca) kernel oil, arginine, arginine aspartate, Arnica montana extract, aspartic acid, avocado (Persea gratissima) oil, barrier sphingolipids, butyl alcohol, beeswax, behenyl alcohol, beta-sitosterol, birch (Betula alba) bark extract, borage (Borago officinalis) extract, butcherbroom (Ruscus aculeatus) extract, butylene glycol, Calendula officinalis extract, Calendula officinalis oil, candelilla (Euphorbia cerifera) wax, canola oil, caprylic / capric triglyceride, cardamom (Elettaria cardamomum) oil, carnauba (Copernicia cerifera) wax, carrot (Daucus carota sativa) oil, castor (Ricinus communis) oil, ceramides, ceresin, ceteareth-5, ceteareth-12, ceteareth-20, cetearyl octanoate, ceteth-20, ceteth-24, cetyl acetate, cetyl octanoate, cetyl palmitate, chamomile (Anthemis nobilis) oil, cholesterol, cholesterol esters, cholesteryl hydroxystearate, citric acid, clary (Salvia sclarea) oil, cocoa (Theobroma cacao) butter, coco-caprylate / caprate, coconut (Cocos nucifera) oil, collagen, collagen amino acids, com (Zea mays) oil, fatty acids, decyl oleate, dimethicone copolyol, dimethiconol, dioctyl adipate, dioctyl succinate, dipentaerythrityl hexacaprylate / hexacaprate, DNA, erythritol, ethoxydiglycol, ethyl linoleate, Eucalyptus globulus oil, evening primrose (Oenothera biennis) oil, fatty acids, Geranium maculatum oil, glucosamine, glucose glutamate, glutamic acid, glycereth-26, glycerin, glycerol, glyceryl distearate, glyceryl hydroxystearate, glyceryl laurate, glyceryl linoleate, glyceryl myristate, glyceryl oleate, glyceryl stearate, glyceryl stearate SE, glycine, glycol stearate, glycol stearate SE, glycosaminoglycans, grape (Vitis vinifera) seed oil, hazel (Corylus americana) nut oil, hazel (Corylus avellana) nut oil, hexylene glycol, hyaluronic acid, hybrid safflower (Carthamus tinctorius) oil, hydrogenated castor oil, hydrogenated coco-glycerides, hydrogenated coconut oil, hydrogenated lanolin, hydrogenated lecithin, hydrogenated palm glyceride, hydrogenated palm kernel oil, hydrogenated soybean oil, hydrogenated tallow glyceride, hydrogenated vegetable oil, hydrolyzed collagen, hydrolyzed elastin, hydrolyzed glycosaminoglycans, hydrolyzed keratin, hydrolyzed soy protein, hydroxylated lanolin, hydroxyproline, isocetyl stearate, isocetyl stearoyl stearate, isodecyl oleate, isopropyl isostearate, isopropyl lanolate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, isostearamide DEA, isostearic acid, isostearyl lactate,299472458.1 - 34 - OLAP.POOQ9WO / 1001359125isostearyl neopentanoate, jasmine (Jasminum officinale) oil,jojoba (Buxus chinensis) oil, kelp, kukui (Aleurites moluccana) nut oil, lactamide MEA, laneth-16, laneth-10 acetate, lanolin, lanolin acid, lanolin alcohol, lanolin oil, lanolin wax, lavender (Lavandula angustifolia) oil, lecithin, lemon (Citrus medica limonum) oil, linoleic acid, linolenic acid, Macadamia ternifolia nut oil, maltitol, matricaria (Chamomilla recutita oil, methyl glucose sesqui stearate, methylsilanol PCA, mineral oil, mink oil, mortierella oil, myristyl lactate, myristyl myristate, myristyl propionate, neopentyl glycol dicaprylate / dicaprate, octyldodecanol, octyldodecyl myristate, octyldodecyl stearoyl stearate, octyl hydroxystearate, octyl palmitate, octyl salicylate, octyl stearate, oleic acid, olive (Olea europaea) oil, orange (Citrus aurantium dulcis) oil, palm (Elaeis guineensis) oil, palmitic acid, pantethine, panthenol, panthenyl ethyl ether, paraffin, PCA, peach (Prunus persica) kernel oil, peanut (Arachis hypogaea) oil, PEG-8 Cl 2- 18 ester, PEG- 15 cocamine, PEG- 150 distearate, PEG-60 glyceryl isostearate, PEG-5 glyceryl stearate, PEG-30 glyceryl stearate, PEG-7 hydrogenated castor oil, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-20 methyl glucose sesqui stearate, PEG-40 sorbitan peroleate, PEG-5 soy sterol, PEG- 10 soy sterol, PEG-2 stearate, PEG-8 stearate, PEG- 20 stearate, PEG-32 stearate, PEG-40 stearate, PEG-50 stearate, PEG- 100 stearate, PEG- 150 stearate, pentadecalactone, peppermint (Mentha piperita) oil, petrolatum, phospholipids, plankton extract, polyamino sugar condensate, polyglyceryl-3 diisostearate, polyquaternium- 24, polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, polysorbate 85, potassium myristate, potassium palmitate, propylene glycol, propylene glycol dicaprylate / dicaprate, propylene glycol dioctanoate, propylene glycol dipelargonate, propylene glycol laurate, propylene glycol stearate, propylene glycol stearate SE, PVP, pyridoxine dipalmitate, retinol, retinyl palmitate, rice (Oryza sativa) bran oil, RNA, rosemary (Rosmarinus officinalis) oil, rose oil, safflower (Carthamus tinctorius) oil, sage (Salvia officinalis) oil, sandalwood (Santalum album) oil, serine, serum protein, sesame (Sesamum indicum) oil, shea butter (Butyrospermum parkii), silk powder, sodium chondroitin sulfate, sodium hyaluronate, sodium lactate, sodium palmitate, sodium PCA, sodium polyglutamate, soluble collagen, sorbitan laurate, sorbitan oleate, sorbitan palmitate, sorbitan sesquioleate, sorbitan stearate, sorbitol, soybean (Glycine soja) oil, sphingolipids, squalane, squalene, stearamide MEA-stearate, stearic acid, stearoxy dimethicone, stearoxytrimethylsilane, stearyl alcohol, stearyl glycyrrhetinate, stearyl heptanoate, stearyl stearate, sunflower (Helianthus annuus) seed oil, sweet almond (Prunus amygdalus dulcis) oil, synthetic beeswax, tocopherol, tocopheryl acetate, tocopheryl linoleate, tribehenin, tridecyl neopentanoate, tridecyl stearate, triethanolamine, tristearin, urea, vegetable oil, water, waxes, wheat (Triticum vulgar e) germ oil, and ylang ylang (Cananga odorata) oil.299472458.1 - 35 - OLAP.POOQ9WO / 1001359125L. Thickening Agents / Viscosity Modifying Agents

[0090] Thickening agents, including thickener or gelling agents, include substances which that can increase the viscosity of a composition. Thickeners includes those that can increase the viscosity of a composition without substantially modifying the efficacy of the active ingredient within the composition. Thickeners can also increase the stability of the compositions of the present invention. In certain aspects of the present invention, thickeners include hydrogenated polyisobutene, trihydroxystearin, ammonium acryloyldimethyltaurate / vp copolymer, or a mixture of them.

[0091] Non-limiting examples of additional thickening agents that can be used in the present compositions include carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides, and gums. Examples of carboxylic acid polymers include crosslinked compounds containing one or more monomers derived from acrylic acid, substituted acrylic acids, and salts and esters of these acrylic acids and the substituted acrylic acids, wherein the crosslinking agent contains two or more carbon-carbon double bonds and is derived from a polyhydric alcohol (see U.S. Pat. Nos. 5,087,445; 4,509,949; 2,798,053; CTFA International Cosmetic Ingredient Dictionary, Fourth edition, 1991, pp. 12 and 80). Examples of commercially available carboxylic acid polymers include carbomers, which are homopolymers of acrylic acid crosslinked with allyl ethers of sucrose or pentaerytritol (e.g., Carbopol™ 900 series from B. F. Goodrich).

[0092] Non-limiting examples of crosslinked polyacrylate polymers include cationic and nonionic polymers. Examples are described in U.S. Pat. Nos. 5,100,660; 4,849,484; 4,835,206; 4,628,078; 4,599,379).

[0093] Non-limiting examples of polyacrylamide polymers (including nonionic polyacrylamide polymers including substituted branched or unbranched polymers) include polyacrylamide, isoparaffin and laureth-7, multi-block copolymers of acrylamides and substituted acrylamides with acrylic acids and substituted acrylic acids.

[0094] Non-limiting examples of polysaccharides include cellulose, carboxymethyl hydroxyethylcellulose, cellulose acetate propionate carboxylate, hydroxyethylcellulose, hydroxyethyl ethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose, methyl hydroxyethylcellulose, microcrystalline cellulose, sodium cellulose sulfate, and mixtures thereof. Another example is an alkyl substituted cellulose where the hydroxy groups of the cellulose polymer is hydroxyalkylated (preferably hydroxy ethylated or hydroxypropylated) to form a hydroxyalkylated cellulose which is then further modified with a CIO -C30 straight chain or branched chain alkyl group through an ether linkage. Typically these polymers are299472458.1 - 36 - OLAP.POOQ9WO / 1001359125ethers of C10-C30 straight or branched chain alcohols with hydroxyalkylcelluloses. Other useful polysaccharides include scleroglucans comprising a linear chain of (1-3) linked glucose units with a (1-6) linked glucose every three unit.

[0095] Non-limiting examples of gums that can be used with the present invention include acacia, agar, algin, alginic acid, ammonium alginate, amylopectin, calcium alginate, calcium carrageenan, carnitine, carrageenan, dextrin, gelatin, gellan gum, guar gum, guar hydroxypropyltrimonium chloride, hectorite, hyaluronic acid, hydrated silica, hydroxypropyl chitosan, hydroxypropyl guar, karaya gum, kelp, locust bean gum, natto gum, potassium alginate, potassium carrageenan, propylene glycol alginate, sclerotium gum, sodium alginate, sodium carboxymethyl dextran, sodium carrageenan, tragacanth gum, xanthan gum, and mixtures thereof.

[0096] Viscosity modifying agents and / or additional thickening agents include, but are not limited to, viscous liquids, such as polyethylene glycol, semisynthetic polymers, such as semisynthetic cellulose derivatives, synthetic polymers, poloxamers, and polyethyleneimines (e.g., PEI-10), petroleum jelly, waxes, particulate associate colloids, such as bentonite and / or colloidal silicon dioxide, PPG-2 hydroxyethyl coco / isostearamide, disteareth-75 IPDI, and salts, such as sodium chloride, and combinations thereof.M. Emollients

[0097] Suitable emollients for use in the formulations include, but are not limited to a silicone compound (e.g., dimethicone, cyclomethicone, dimethicone copolyol or a mixture of cyclopentasiloxane and dimethicone / vinyldimethicone cross polymer, cyclopentasiloxane polysilicone), polyols such as sorbitol, glycerin, propylene glycol, ethylene glycol, polyethylene glycol, caprylyl glycol, polypropylene glycol, 1,3-butane diol, hexylene glycol, isoprene glycol, xylitol; ethylhexyl palmitate; a triglyceride such as caprylic / capric triglyceride and fatty acid ester such as cetearyl isononanoate or cetyl palmitate. In a specific embodiment, the emollient is dimethicone, amidodimethicone, dimethiconol, cyclopentasiloxane, potassium dimethicone PEG-7 panthenyl phosphate, or a combination thereof. In some instances, the composition(s) do not contain a silicone compound.

[0098] The emollients are optionally included in an amount ranging from about 0.001% to about 20% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45, 0.46, 0.47,299472458.1 - 37 - OLAP.POOQ9WO / 10013591250.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62, 0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96, 0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 wt. %.N. Conditioning Agents

[0099] Conditioning agents include, but are not limited to, silicone-based agents (e.g., silicone quaternium-8), panthenol, hydrolyzed wheat and / or soy protein, amino acids (e.g. wheat amino acids), rice bran wax, meadowfoam seed oil, mango seed oil, grape seed oil, jojoba seed oil, sweet almond oil, hydroxy ethyl behenamidopropyl dimonium chloride, aloe leaf extract, aloe barbadensis leaf juice, phytantriol, panthenol, retinyl palmitate, behentrimonium methosulfate, cyclopentasiloxane, quatemium-91, stearamidopropyl dimethylamine, cationic polymers derived from polysaccharides, for example cationic cellulose derivatives, cationic starch derivatives, cationic guar derivatives and cationic locust bean gum derivatives, synthetic cationic polymers, mixtures or combinations of these agents. The formulation may comprise other synthetic or natural polymers or polymers derived from biological preparation processes, which are functionalized, where appropriate, for example with cationic or neutral groups. These polymers may have a stabilizing or strengthening action on the compositions, and / or a conditioning action (deposition on the surface of the skin or the hair), and combinations thereof. In some instances, the composition(s) do not contain a silicone compound.

[0100] The conditioning agents are optionally included in an amount ranging from aboutO.001% to about 60% by weight of the formulation, such as equal to, more than, less than, a range of, or between 0.001, 0.005, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.11, 0.12, 0.13, 0.14, 0.15, 0.16, 0.17, 0.18, 0.19, 0.2, 0.21, 0.22, 0.23, 0.24, 0.25, 0.26, 0.27, 0.28, 0.29, 0.3, 0.31, 0.32, 0.33, 0.34, 0.35, 0.36, 0.37, 0.38, 0.39, 0.4, 0.41, 0.42, 0.43, 0.44, 0.45,0.46, 0.47, 0.48, 0.49, 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59, 0.6, 0.61, 0.62,0.63, 0.64, 0.65, 0.66, 0.67, 0.68, 0.69, 0.7, 0.71, 0.72, 0.73, 0.74, 0.75, 0.76, 0.77, 0.78, 0.79, 0.8, 0.81, 0.82, 0.83, 0.84, 0.85, 0.86, 0.87, 0.88, 0.89, 0.9, 0.91, 0.92, 0.93, 0.94, 0.95, 0.96,0.97, 0.98, 0.99, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 40, 45, 50, 55, or 60 wt. %.O. Opacifying Agents

[0101] Opacifying agents include, but are not limited to, glycol distearate and ethoxylated fatty alcohols.299472458.1 - 38 - OLAP.POOQ9WO / 1001359125P. Propellant

[0102] Propellants include, but are not limited to, a liquefiable gas or a halogenated propellant. Examples of suitable propellants include dimethyl ether and hydrocarbon propellants such as propane, n-butane, iso-butane, CFCs, and CFC-replacement propellants. The propellants may be used singly or admixed.

[0103] The amount of propellant may range from about 10% to about 60% by weight of the formulation such as equal to, more than, less than, a range of, or between 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, or 60 wt. %.

[0104] The propellant may be separated from the hair formulation as in a two compartment container. Other suitable aerosol dispensers are those characterized by the propellant being compressed air, which can be filled into the dispenser using a pump or equivalent device prior to use. Conventional non-aerosol pump spray dispensers, i.e., atomizers, may also be used to apply the hair strengthening formulation to the hair.Q. Pharmaceutically-Active Agents

[0105] Pharmaceutically-active agents are also contemplated as being useful with the present compositions. Non-limiting examples of pharmaceutical active agents include antibiotics, antifungals, antivirals, antimicrobials, biologically active proteins and peptides, depigmenting agents, hair growth stimulants, hair growth retardants including DFMO and its salts and analogs, steroids including hormones and corticosteroids, etc.R. Amounts of Ingredients

[0106] It is contemplated that the compositions of the present invention can include any amount of the ingredients discussed in this specification. The compositions can also include any number of combinations of additional ingredients described throughout this specification (e.g., pigments, or conditioning agents or pharmaceutical ingredients). The concentrations of the any ingredient within the compositions can vary. In non-limiting embodiments, for example, the compositions can comprise, consisting essentially of, or consist of, in their final form, for example, at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%, 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%, 0.0044%, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%,299472458.1 - 39 - OLAP.POOQ9WO / 10013591250.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0.3250%, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.0550%, 0.5750%, 0.6000%, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%,2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%,4.0%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%,5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%,6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%,8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%,9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% or any range derivable therein, of at least one of the ingredients that are mentioned throughout the specification and claims. In non-limiting aspects, the percentage can be calculated by weight or volume of the total composition. A person of ordinary skill in the art would understand that the concentrations can vary depending on the addition, substitution, and / or subtraction of ingredients in a given composition.S. Carriers

[0107] The compositions of the present invention can include or be incorporated into all types of carriers and vehicles. The carrier or vehicle can be a pharmaceutically or dermatologically acceptable carrier or vehicle. Non-limiting examples of carriers or vehicles include water, glycerin, alcohol, oil, a silicon containing compound, a silicone compound, and wax. Variations and other appropriate vehicles will be apparent to the skilled artisan and are appropriate for use in the present invention. In certain aspects, the concentrations and combinations of the compounds, ingredients, and agents can be selected in such a way that the299472458.1 - 40 - OLAP.POOQ9WO / 1001359125combinations are chemically compatible and do not form complexes which precipitate from the finished product.T. Formulations

[0108] The compositions of the present invention can be structured or formulated into a variety of different forms. Non-limiting examples include emulsions (e.g., water-in-oil, water- in-oil-in-water, oil-in-water, silicone-in-water, water-in-silicone, oil-in-water-in-oil, oil-in- water-in-silicone emulsions), creams, lotions, solutions (both aqueous and hydro-alcoholic), anhydrous bases (such as powders), gels, masks, and ointments. Variations and other structures will be apparent to the skilled artisan and are appropriate for use in the present invention.U. Method of Use

[0109] In one embodiment, prior to treatment with the methods and compositions described herein, the hair has been damaged, such as hair that at least some of the thiol groups in the hair are free thiols. The methods and compositions can be applied to the hair to bind the free thiol groups. Preferably, the methods and / or compositions are applied at least within one week of the hair being damaged, preferably within three days, more preferably within two days, most preferably, the same day.1. Rinse or Wash the Hair

[0110] Optionally, the hair may be shampooed and / or conditioned prior to applying the methods and / or compositions. Alternately, the hair may only be rinsed with water prior to application of the methods and / or compositions.2. Apply the methods and / or compositions to the Hair[OHl] In some instances, subsequent to shampooing, conditioning, and / or rinsing the hair, the method and / or composition is applied to the hair. Alternately, the hair does not have to be washed or rinsed prior to application of the method and / or composition. In some embodiments, the method and / or composition is applied to dry hair.

[0112] The compositions may be used on hair daily, weekly, monthly, yearly or any amount of time therein.

[0113] Typically, the amount of compositions applied is sufficient to saturate the hair.

[0114] The methods and / or compositions may be applied to the hair as a single application, or application may be repeated one or more times. Typically, the amount of composition applied in each application is sufficient to saturate the hair. The volume of the composition(s) applied to the hair in each application may be about 1 to about 100 mL per person depending299472458.1 - 41 - OLAP.POOQ9WO / 1001359125on their length and volume of hair, such as equal to, more than, less than, a range of, or between 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78,79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 ml. In some embodiments, application of the composition could be repeated immediately (e.g. within about 10 to 15 seconds) or between about one and five minutes, greater than five minutes, between about five and ten minutes, greater than ten minutes, between about ten and twenty (20) minutes after the first application, such as equal to, more than, less than, a range of, or between 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 minutes.3. Remove the Composition(s) from the Hair

[0115] In some instances, the hair is washed or rinsed subsequent to the application of the methods and / or compositions after the treatment period is over if a treatment period is used. The hair may be rinsed and subsequently washed immediately (e.g. within 10, 15, 25, 30, 45, 60 seconds (one minute), two minutes, three minutes, four, or five minutes following application) after final application of the composition(s). Alternatively the hair may be rinsed and / or washed within about 3 hours following application, such as 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, or 1 hour and 55 minutes, 2 hours, 2 hours and 5 minutes, 2 hours and 10 minutes, 2 hours and 15 minutes, 2 hours and 20 minutes, 2 hours and 25 minutes, 2 hours and 30 minutes, 2 hours and 35 minutes, 2 hours and 40 minutes, 2 hours and 45 minutes, 2 hours and 50 minutes, 2 hours and 55 minutes, or 3 hours.

[0116] Alternately, the hair does not have to be washed or rinsed subsequent to application of the compositions and / or methods. In some instances, the first composition is not completely rinsed and / or washed from the hair before application of the second composition. In some instances, the first composition is not completely rinsed and / or washed from the hair before application of the third composition. In some instances, the second composition is not completely rinsed and / or washed from the hair before application of the third composition. In some instances, the first composition and the second composition are not completely rinsed and / or washed from the hair before application of the third composition.299472458.1 - 42 - OLAP.POOQ9WO / 1001359125

[0117] In some instances, the third composition covalently binds latent free thiols in the hair. The thiols remain bound for at least 1, 2, 3, 4, 5, or 6 days, or 1, 2, 3, 4, 5, 6, 7, 8, 8, 10, 11, or 12 weeks. The binding reaction is a stable reaction, such that the thiols may remain bound even if subjected to a hair coloring treatment (simultaneous or subsequent to the binding reaction).4. Chemical Treatment of Hair with a Reducing Agent

[0118] In one embodiment, prior to treatment with the method and / or composition, the hair has been subjected to a reducing agent used for waving (also referred to herein as hair perming or permanent waves), curling, and / or straightening of the hair. a. Apply a Reducing Agent to the Hair

[0119] The first step in waving, curling, or straightening hair may be breaking the cysteine disulfide bonds to form free thiol moieties. The process for breaking the cysteine disulfide bonds is via application of a reducing agent. The process for applying the reducing agent involves following normal perming or hair straightening procedures, that are known to those skilled in the art. For example, to perm a hair, the hair is first washed and set on perm rods of various sizes. Second, a reducing agent, such as thioglycolate reducing solution or lotion, is applied to the hair. The hair is allowed to set for a specified period of time, and then the thioglycolate solution is rinsed from the hair.

[0120] The application of hydrogen peroxide in the process of waving, curling, or straightening is optional. In some processes, such as when treating previously chemically treated hair, hydrogen peroxide is generally not used. In other processes, such as when perming virgin hair, hydrogen peroxide may be added. In these embodiments, hydrogen peroxide is typically added after the reducing agent is rinsed out. Then the hydrogen peroxide is rinsed from the hair prior to the method and / or composition. b. Apply the Method(s) and / or Composition(s)

[0121] Subsequent to the reducing treatment, one or more of the first, second, and third composition is / are applied to the hair. Although the methods and / or compositions are typically applied on the same day as treatment with the reducing agent, they may be applied later, such as within 1 to 2 weeks following treatment with the reducing agent.

[0122] In some instances, the hair is washed or rinsed subsequent to the application of the methods and / or compositions after the treatment period is over if a treatment period is used. The hair may be rinsed and subsequently washed immediately (e.g. within 10, 15, 25, 30, 45, 60 seconds (one minute), two minutes, three minutes, four, or five minutes following299472458.1 - 43 - OLAP.POOQ9WO / 1001359125application) after final application of the composition(s). Alternatively the hair may be rinsed and / or washed within about 3 hours following application, such as 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, or 1 hour and 55 minutes, 2 hours, 2 hours and 5 minutes, 2 hours and 10 minutes, 2 hours and 15 minutes, 2 hours and 20 minutes, 2 hours and 25 minutes, 2 hours and 30 minutes, 2 hours and 35 minutes, 2 hours and 40 minutes, 2 hours and 45 minutes, 2 hours and 50 minutes, 2 hours and 55 minutes, or 3 hours.

[0123] Alternately, the hair does not have to be washed or rinsed subsequent to application of the compositions and / or methods. In some instances, the first composition is not completely rinsed and / or washed from the hair before application of the second composition. In some instances, the first composition is not completely rinsed and / or washed from the hair before application of the third composition. In some instances, the second composition is not completely rinsed and / or washed from the hair before application of the third composition. In some instances, the first composition and the second composition are not completely rinsed and / or washed from the hair before application of the third composition.

[0124] The compositions described herein improve hair quality, such as appearance (e.g., sheen) and feel, and decrease hair breakage without decreasing strength (e.g., tensile strength) when the hair is subjected to subsequent treatments, such as coloring.

[0125] In some embodiments, hair breakage decreases by 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, or 75% or higher after treatment with the method(s) and / or composition(s) compared to untreated hair from the same individual. Hair breakage is a significant problem encountered during coloring and other treatments.5. Apply Coloring Formulation to the Hair

[0126] In some instances, the methods and / or compositions are applied to color treated and / or colored hair, such as dyed hair. In some instances, the hair is color treated and / or colored subsequent to the application of the method(s) and / or composition(s).

[0127] Coloring formulation are generally applied to an individual's hair following normal hair coloring procedures that are known to those skilled in the art. Typically, hair color treatments include two complementary processes: bleaching the hair's natural pigment and / or other artificial pigments present in the hair, and diffusion of dye precursors into the hair,299472458.1 - 44 - OLAP.POOQ9WO / 1001359125followed by coupling reactions that result in the formation of chromophores within the hair shaft, which are too large to diffuse out of the hair. The hair coloring formulation may be a highlighting formulation, such as formed by mixing bleach powder and developer. More complex colors may contain several precursors and many couplers, and may involve multiple reactions.

[0128] The dye precursors may contain several ingredients, each with different functions. The first ingredient is usually an alkalizing agent (usually ammonia and / or an ammonia substitute, such as monoethanol amine [MEA]). The alkalizing agent serves a number of roles in the hair colorant process including swelling the hair fiber to aid in diffusion of the dye precursors. The dye precursors generally include p-diamines and p-aminophenols. Precursors are oxidized to active intermediates once they have penetrated the hair shaft. Intermediates then react with color couplers to create wash resistant dyes. More specifically, the intermediates, in the presence of an oxidant, couple with another oxidation dye intermediate molecule to form a large fused ring color compound within the hair shaft. The precursor intermediate should penetrate the hair shaft prior to the coupling reaction since the fused ring product is too large to penetrate the hair shaft. Couplers modify the color produced by the oxidation of precursor compounds. The primary difference between demi-permanent and permanent products is the alkalizing agent and the concentration of peroxide. The cuticle does not swell as greatly with demi-permanent dyes, making dye penetration less efficient compared to permanent coloring products.

[0129] Several coloring formulations use a reducing agent, such as sodium bisulfate, to break disulfide bonds in the hair, allowing deeper penetration of the hair coloring dyes into the hair. Specifically, the method includes reducing some of the disulfide linkages of the cysteine in the hair shafts to thiol groups while breaking hydrogen bonds. The reducing process changes the chemical and cosmetic characteristics of the hair, which are undesirable.

[0130] The hair dyeing process may be followed by a shampoo and conditioning treatment, a neutralizing rinse or an acid balanced shampoo containing in addition to cationic or amphoteric surfactants, cation-active emollients and quaternary polymers. Alternately, the hair dyeing process may be followed by application of the method(s) and / or composition(s) described herein, before and / or after a shampoo and / or conditioning treatment. a. Applying Binding Formulation

[0131] The method(s) and / or composition(s) may be applied simultaneously with hair coloring formulation or subsequent to the application of the hair coloring formulation. For299472458.1 - 45 - OLAP.POOQ9WO / 1001359125example, the third composition may be mixed with the hair coloring treatment and the mixture, containing both the third composition and the hair coloring treatment, may be applied to the hair.

[0132] Alternatively, subsequent to coloring the hair, the method(s) and / or composition(s) is / are applied to the hair. Although the methods and / or compositions are typically applied on the same day as coloring the hair, they may be applied later, such as within 1 to 2 weeks following coloring the hair.

[0133] In some instances, the hair is washed or rinsed subsequent to the coloring of the hair and before application of the methods and / or compositions. The hair may be rinsed and subsequently washed immediately (e.g., within 10, 15, 25, 30, 45, 60 seconds (one minute), two minutes, three minutes, four, or five minutes following application) after final application of the composition(s). Alternatively the hair may be rinsed and / or washed within about 3 hours following application, such as 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, 1 hour, 1 hour and 5 minutes, 1 hour and 10 minutes, 1 hour and 15 minutes, 1 hour and 20 minutes, 1 hour and 25 minutes, 1 hour and 30 minutes, 1 hour and 35 minutes, 1 hour and 40 minutes, 1 hour and 45 minutes, 1 hour and 50 minutes, or 1 hour and 55 minutes, 2 hours, 2 hours and 5 minutes, 2 hours and 10 minutes, 2 hours and 15 minutes, 2 hours and 20 minutes, 2 hours and 25 minutes, 2 hours and 30 minutes, 2 hours and 35 minutes, 2 hours and 40 minutes, 2 hours and 45 minutes, 2 hours and 50 minutes, 2 hours and 55 minutes, or 3 hours.

[0134] Alternately, the hair does not have to be washed or rinsed subsequent to coloring the hair.

[0135] The compositions described herein improve hair quality, such as appearance (e.g., sheen) and feel and decrease hair breakage without decreasing dry strength (e.g., tensile strength) when the hair is subjected to subsequent treatments, such as coloring.

[0136] In some embodiments, hair breakage decreases by 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, or 75% or higher after treatment with the method(s) and / or composition(s) compared to untreated hair from the same individual. Hair breakage is a significant problem encountered during coloring and other treatments.

[0137] If the first, second, and / or third composition is combined with the hair coloring treatment and applied as a mixture to the hair, then the mixture remains on the hair as long as needed for the hair coloring treatment. Typically, the mixture is applied for approximately 10 minutes. The mixture is removed from the hair in accordance with standard methods for hair299472458.1 - 46 - OLAP.POOQ9WO / 1001359125coloring treatments, e.g., rinse and shampoo, approximately 10 minutes after applying the mixture.

[0138] The composition(s) may be rinsed from the hair after application. The hair may be rinsed and subsequently washed immediately (e.g., within 10 to 15 seconds following application) after final application of the composition(s). In some instances, the hair is rinsed and / or washed about 10 minutes or later after the final application of the composition(s), such as about 15 minutes to about 30 minutes.V. Kits

[0139] Kits are also contemplated as being used in certain aspects of the present invention. For instance, compositions of the present invention can be included in a kit. A kit can include a container. Containers can include a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressurized container, a barrier container, a package, a compartment, or other types of containers such as injection or blow-molded plastic containers. The kit and / or container can include indicia on its surface. The indicia, for example, can be a word, a phrase, an abbreviation, a picture, or a symbol. In some instances, the kit can include two ore more containers with different compositions in each container to implement the multi-step processes of the present invention.

[0140] The containers can dispense a pre-determined amount of the composition. In other embodiments, the container can be squeezed (e.g., metal, laminate, or plastic tube) to dispense a desired amount of the composition. The composition can be dispensed as a spray, an aerosol, a liquid, a fluid, or a semi-solid. The containers can have spray, pump, or squeeze mechanisms. A kit can also include instructions for employing the kit components as well the use of any other compositions included in the container. Instructions can include an explanation of how to apply, use, and maintain the compositions.EXAMPLES

[0141] The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.299472458.1 - 47 - OLAP.POOQ9WO / 1001359125

[0142] All of the compositions and / or methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the compositions and / or methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.A. Example 1 (Exemplary Formulations)

[0143] Formulations were prepared as hair compositions. The formulation in Table 1 is an example of a first composition. The formulation in Table 2 is an example of a second composition. The formulations in Table 3 and Table 4 are examples of a third composition. Each of the formulations disclosed herein may be used independently, e.g., independently of use of the other formulations herein and / or independently of the methods disclosed herein.Table 1*Formulation can be prepared by mixing the ingredients in a beaker under heat 70-75°C until homogenous. Subsequently, the formulation can be cooled to standing room temperature (20-25°C). Further, and if desired, additional ingredients can be added, for example, to modify the rheological properties of the composition.**Excipients can be added, for example, to modify the rheological properties of the composition.

[0144] Alternatively, the amount of water can be varied so long as the amount of water in the composition is at least 70% w / w, and preferably between 70 to 99% w / w.Table 2299472458.1 - 48 - OLAP.POOQ9WO / 1001359125*Formulation can be prepared by mixing the ingredients in a beaker under heat 70-75°C until homogenous. Subsequently, the formulation can be cooled to standing room temperature (20-25°C). Further, and if desired, additional ingredients can be added, for example, to modify the rheological properties of the composition.**Excipients can be added, for example, to modify the rheological properties of the composition.

[0145] Alternatively, the amount of water can be varied so long as the amount of water in the composition is at least 70% w / w, and preferably between 70 to 99% w / w.Table 3*Formulation can be prepared by mixing the ingredients in a beaker under heat 70-75°C until homogenous. Subsequently, the formulation can be cooled to standing room temperature (20-25°C). Further, and if desired, additional ingredients can be added, for example, to modify the rheological properties of the composition.**Excipients can be added, for example, to modify the rheological properties of the composition.

[0146] Alternatively, the amount of water can be varied so long as the amount of water in the composition is at least 70% w / w, and preferably between 70 to 98% w / w.Table 4299472458.1 - 49 - OLAP.POOQ9WO / 1001359125*Formulation can be prepared by mixing the ingredients in a beaker under heat 70-75°C until homogenous. Subsequently, the formulation can be cooled to standing room temperature (20-25°C). Further, and if desired, additional ingredients can be added, for example, to modify the rheological properties of the composition. **Excipients can be added, for example, to modify the rheological properties of the composition.

[0147] Alternatively, the amount of water can be varied so long as the amount of water in the composition is at least 70% w / w, and preferably between 70 to 98% w / w.B. Example 2 (Testing on Hair)

[0148] A non-limiting example of a method disclosed herein using non-limiting examples of a first composition, a second composition, and a third composition disclosed herein. It was determined that the method does not change the dry color of dyed hair, decreases the amount of hair breakage, increases the retention of the styled shape of the hair (such as curled hair), decreases the frizzing of hair, and decreases the amount of knots formed in hair / the friction when combing wet hair without decreasing strength of the hair (tensile strength).

[0149] Color Retention: Hair was tested to demonstrate the manner by which the treatment method using the first, second, and third composition changes the color of hair with repeated washing via color testing using a Hunterlab Colorimeter.

[0150] 3 g, 8” long, 1” wide Curly multi-ethnic brown tresses (Walker type 3A), 6% bleached and colored with commercially available auburn dye were tested, ten tresses per treatment group. All of the hair was first bleached by contacting the hair with a skeletal 6% hydrogen peroxide solution that has a pH of 10.2 and a temperature of 40° C at 500 ml per 40 tresses for 40 minutes at 40° C with regular agitation and afterwards rinsed twice with water and then placed under running water. All of the tresses were dyed with L’Oreal Preference shade 4R Dark Auburn dye. The color components were combined using the manufacturer’s instructions and mixed. Then 10 g of the mixture was applied to each tress using a brush (5 g on each side of the tress) and massaged thoroughly into the tress. The hair was maintained at 27° C for 20 minutes and massaged at 10 minutes into the 20 minute treatment. The color treated tresses were rinsed out under running water at a temperature of 40° C for 2 minutes or until the water ran clear. The rinsed hair was allowed to rest for at least 12 hours prior to taking post color treatment readings.

[0151] The tresses were divided into two treatment groups, a Method Treated group and a Control group. The Method Treated group tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of299472458.1 - 50 - OLAP.POOQ9WO / 1001359125Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 60% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo, and were treated as described for the Method Treated procedure but used water instead of any of the first, second, or third composition.

[0152] Color was measured after the respective treatments, after ten washes after the treatment, after twelve washes after the treatment, and after fifteen washes after the treatment. For each wash after the treatment, the 15% SLES solution was applied to the tresses at 10% by weight of the tresses, the hair was massaged for 30 seconds, rested for 5 minutes, rinsed for 30 seconds, and air dried for 48 hours at 60% relative humidity. For the color measurements, CIELAB L, a, and b measurements were taken using a Hunter Lab UltraScan VIS colorimeter. “L” refers to the lightness on a scale of 0 to 100, “a” denotes the red-green color range (positive value denotes higher red), and “b” represents the yellow-blue color range (positive value denotes higher yellow). Changes in the color of the tress were calculated using the difference in these parameters relative to the baseline measurement after treatment (e.g., AL*, Aa*, and Ab*). Overall color change, AE was calculated using the following Equation 1.AE = [AL*2+ Aa*2+ Ab*2] Equation 1

[0153] Ten color measurements were performed on each tress (with ten tresses per treatment). JMP™ analytical software was used to calculate the statistics (student’s t-test at 95% confidence level).

[0154] At the ten, twelve, and fifteen wash intervals, there was no statistical difference between Control and the Method Treated hair. This indicates that the method treatment is safe for color-treated hair.

[0155] Hair Breakage: Hair was tested to demonstrate the ability of the treatment method using the first, second, and third composition to protect hair against breakage via repeated grooming.299472458.1 - 51 - OLAP.POOQ9WO / 1001359125

[0156] 3 g, 8” long, 1” wide Curly multi-ethnic brown tresses (Walker type 3A), 9% bleached were tested, ten tresses per treatment group. All of the hair was first bleached three times by contacting the hair with a 9% hydrogen peroxide solution that has a pH of 10.2 and a temperature of 40° C for 20 minutes at 40° C with regular agitation and rinsed under running water between repeated bleaching and after the third bleaching.

[0157] The tresses were divided into two treatment groups, a Method Treated group and a Control group. The Method Treated group tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 60% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo.

[0158] Determining whether the treatment will protect hair against breakage was tested by repeated grooming experiments. The number of broken fibers was recorded as a function of repeated combing / brushing strokes. A treatment that reduces snags, entanglements, and abrasion can lower the number of broken fibers. Hair breakage by dry grooming was measured by using an automatic groomer for a total of 2,000 cycles. Fibers were collected and counted after every 200 cycles. Eight replicates for each sample were performed. The methodology used has been documented in the scientific literature (Evans & Park, A Statistical Analysis of Hair Breakage. II. Repeated Grooming Experiments, JCosmel.Sci.. 61, 439-455, 2010). JMP™ analytical software was used to calculate the statistics (student’s t-test at 95% confidence level). The percentage reduction in breakage was calculated using the following Equation 2.% Reduction in breakage =(1 - (Breakage of Method Treated / Breakage of Control)) x 100 Equation 2

[0159] A statistical reduction in breakage was seen for the Method Treated group compared to the Control group, with the Method Treated showing an over 55% reduction in breakage.299472458.1 - 52 - OLAP.POOQ9WO / 1001359125

[0160] Tensile Strength: Hair was tested to demonstrate the ability of the treatment method using the first, second, and third composition to not negatively impact the fiber integrity of hair via a miniature tensile tester (MTT) of wet hair.

[0161] 3 g, 8” long, 1” wide Curly multi-ethnic brown tresses (Walker type 3A) were tested, two tresses per treatment group. 50 hair fibers per treatment were evaluated using the MTT.

[0162] The tresses were divided into two treatment groups, a Method Treated group and a Control group. The Method Treated group tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 60% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo.

[0163] Determining whether the treatment will not reduce tensile strength was tested using a Dia-Stron Mini Tensile Tester (MTT). The MTT extends individual hair fibers at a rate of 40 mm / minute until break. The force required over the extension distance of the hair is recorded to create stress-strain curves. The rather complex mechanical properties of hair can be a direct consequence of its composite structure. Changes in these properties can be a reflection of an alteration in structure. The changes may be permanent changes, such as those that result from chemical treatments that deplete strength-supporting bonds; or temporary changes, such as those occurring due to changing levels of plasticization that result from the moisture content of hair being dictated by the relative humidity. Tensile experiments are widely regarded to reflect the properties of the inner-most portions of the hair structure (the cortex) and may have no significant contribution from the outer cuticle layer. Instead, the outer portions of the hair structure are generally considered to play a greater role in bending and twisting (torsion). Therefore, surface coatings or materials that only penetrate the outer portions of the hair may be expected to register larger effects in bending and twisting (torsion) than in tensile strength.299472458.1 - 53 - OLAP.POOQ9WO / 1001359125JMP™ analytical software was used to calculate the statistics (student’s t-test at 95% confidence level). A variety of tensile parameters were reported to illustrate the overall condition of the hair.

[0164] The results show that the Method Treated group does not lead to statistical changes in the tensile properties of hair. This indicates that the methods and compositions herein do not negatively impact the mechanical properties of hair. The results show that there are no statistical differences in cross sectional area mean. The results show there are no statistical differences in break stress that represents the force / area needed to break the hair fiber: the higher break stress, the stronger the fiber. The results show there are no statistical differences in the slope of the initial portion of the stress-strain curve adjusted for the cross-sectional area, termed the Young’s modulus. The Young’s modulus represents the elastic stiffness of hair. Also, the results show there are no statistical differences in the break load.

[0165] Curl Droop: Hair was tested to demonstrate the ability of the treatment method using the first, second, and third composition to increase hold of a curl over the course of 8 hours via measurement of tress length using the curl droop method.

[0166] 1 g, 8” long European medium brown straight hair were tested, eight tresses per treatment group.

[0167] The tresses were divided into two treatment groups, a Method Treated group and a Control group. The Method Treated group tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 60% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo.

[0168] Determining whether the treatment will prevent the loss of a curl was tested by measuring the length of the tresses over time. The length of each tress was measured after299472458.1 - 54 - OLAP.POOQ9WO / 1001359125removing from curling overnight by snaking the tresses around large pegs and allowing to equilibrate in 30% relative humidity, and measured after one, two, four, and eight hours of exposure to 60% relative humidity. JMP™ analytical software was used to calculate the statistics (student’s t-test at 95% confidence level). The length of individual tresses is determined using custom written software operating under LAB VIEW™ v8.5. The amount of curl droop for each tress was calculated using Equation 3 where LT = length at any given time; Lo= initial time point length of the tress; and LoL_ = length of the tress in its longest, straight position.Amount of Curl Droop = ((LT - Lo) / (LoL_ - Lo)) Equation 3

[0169] A statistical reduction of loss of curl was seen for the Method Treated group compared to the Control group at all time points measured from 1 hour on. The Method Treated showed an over 55% curl retention at 1 hours of exposure to 60% relative humidity compared to the Control’s under 25 % curl retention at 8 hours of exposure to 60% relative humidity. The Method Treated showed an over 45% curl retention at 8 hours of exposure to 60% relative humidity compared to the Control’s under 20 % curl retention at 8 hours of exposure to 60% relative humidity. This demonstrates the methods and compositions herein ability to retain curl for up to 8 hours in high humidity conditions.

[0170] Hair Frizzing: Hair was tested to demonstrate the ability of the treatment method using the first, second, and third composition to reduce frizzing of hair over the course of 72 hours via measurement of volume of the hair.

[0171] 4 g, 8” long, multi-ethnic frizzy hair (Walker type 3B) were tested, eight tresses per treatment group.

[0172] The tresses were divided into five treatment groups after washing and frizzing to their maximum volume, a Method Treated group with no subsequent washes (Method Treated no wash), a Method Treated group subsequently washed 10 times with a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses (Method Treated + 10), a Method Treated group subsequently washed 12 times with a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses (Method Treated + 12), a Method Treated group subsequently washed 15 times with a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses (Method Treated + 15), and a Control group. The Method Treated groups’ tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the299472458.1 - 55 - OLAP.POOQ9WO / 1001359125shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 20% relative humidity. The subsequent washes for the groups that included subsequent washes, were washed the respective number of times by, for each wash, massaging the hair with sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses, for 30 seconds, resting for 5 minutes, and then rinsing for 30 seconds. After the last wash, the hair was allowed to air dry for 48 hours at 20% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo.

[0173] Determining whether the treatment will prevent frizzing of hair was tested by measuring the volume of the tresses over time. The volume of each tress was measured after frizzing to their maximum volume then treating according to their respective treatment group. The volume of each tress is then measured initially when exposed to 80% relative humidity, and measured after eight, twenty-four, forty-eight, and seventy-two hours of exposure to 80% relative humidity. JMP™ analytical software was used to calculate the statistics (student’s t- test at 95% confidence level). The volume of individual tresses is determined using custom written software operating under LAB VIEW™ v8.5. The amount of volume for each tress was calculated by counting pixels and the percent reduction in frizz of a tress is obtained by using Equation 4.% Reduction in Frizz = ((Max Frizz-Treatment Frizz) / Max Frizz) x 100 Equation 4

[0174] A statistical reduction frizzing was seen for each of the Method Treated groups compared to the Control group at all time points measured from initial exposure to 80% relative humidity on. For example, the Method Treated no washing showed over 70% frizz reduction at 72 hours of exposure to 80% relative humidity and the Method Treated +15 showed over 50% frizz reduction at 72 hours of exposure to 80% relative humidity even though it was washed 15 times after treatment compared to the Control’s under 45% frizz reduction at 72299472458.1 - 56 - OLAP.POOQ9WO / 1001359125hours of exposure to 80% relative humidity. As another example, the Method Treated no washing showed over 75% frizz reduction at initial exposure to 80% relative humidity and the Method Treated +15 showed over 50% frizz reduction at initial exposure to 80% relative humidity even though it was washed 15 times after treatment compared to the Control’s under 40% frizz reduction at initial exposure to 80% relative humidity. This demonstrates the methods and compositions herein ability to reduce frizz immediately and for up to 72 hours in high humidity conditions, even through 15 washes.

[0175] Wet Combing: Hair was tested to demonstrate the ability of the treatment method using the first, second, and third composition to smooth damaged hair via wet combing by the Garcia Method.

[0176] 3 g, 8” long, 1” wide Curly multi-ethnic brown tresses (Walker type 3A), 6% bleached were tested, eight tresses per treatment group. All of the hair was first bleached by contacting the hair with a skeletal 6% hydrogen peroxide solution that has a pH of 10.2 and a temperature of 40° C at 500 ml per 40 tresses for 40 minutes at 40° C with regular agitation and afterwards rinsed twice with water and then placed under running water.

[0177] The tresses were divided into two treatment groups, a Method Treated group and a Control group. The Method Treated group tresses were 1) washed with OLAPLEX™ 4C Shampoo by applying the shampoo at 10% by weight of the tresses (0.1 ml / g of hair), massaged for 30 seconds, rested for 5 minutes, and then rinsed for 30 seconds, 2) the first composition of Table 1 was applied to the damp hair at 20% by weight of the tresses (0.2 ml / g of hair), massaged for 30 seconds, and rested for 10 minutes, 3) without rinsing, the second composition of Table 2 was applied to the damp hair by spraying onto the hair three times (top, middle, bottom) from a distance of 6 inches on one side of the tresses and repeated on the other side of the tresses, and rested for 10 minutes, and 4) without rinsing, the third composition of Table 3 was applied to the damp hair at 15% by weight of the tresses, massaged for 30 seconds, and rested and allowed to air dry for 48 hours at 60% relative humidity. A Control group of tresses were washed as described for the Method Treated procedure but used a sodium laureth sulfate (SLES) solution containing 15% SLES, the solution being used at 10% by weight of the tresses for the shampoo.

[0178] Determining whether the treatment will smooth the hair was tested by an Instron tensile tester to measure frictional forces while a wet hair tress is pulled through a comb. A treatment that smooths hair can lower the frictional forces. Six replicates for each sample were performed. The methodology used has been documented in the scientific literature (Garcia & Diaz, JSCC, 27, (1976) 379-398). JMP™ analytical software was used to calculate the299472458.1 - 57 - OLAP.POOQ9WO / 1001359125statistics (student’s t-test at 95% confidence level). The percentage reduction in combing forces was calculated using the following Equation 5.% Reduction in Combing Forces =(1 - (Combing Force of Treatment / Combing Force of Control)) x 100 Equation 5

[0179] A statistical reduction in friction was seen for the Method Treated group compared to the Control group, with the Method Treated showing a 20 or greater % reduction in wet combing force compared with the control. This demonstrates that the methods and compositions herein improve manageability and provide detangling benefits and lower combing friction.C. Example 3 (Clinical Studies)

[0180] In a clinical study, it has been determined that the methods and composition disclosed herein can, among other things, enhance natural wave, curl, coil definition, can help hair look repaired, healthier, and less damaged, and can restore curl elasticity, bounce, and curl retention. A summary of results are found in Tables 4 to 7 and the outline of the clinical study methods and results used to determine the properties of the ingredients are provided below.

[0181] Immediately after treatment stylists and consumers were required to complete a survey about the products’ attributes. Forty-eight hours after treatment, consumers were required to take a second survey about the products’ attributes. Sixty-eight participants between the ages 18-60 completed the study. Individuals with self-perceived light to moderately damaged (chemical, color treated, thermal) type 2A-4C waves, curls and coils- with the focus on type 3 and type 4 participated. 15% of the participants had Type 2B / 2C hair, 65% had Type 3 hair, and 20% had Type 4 hair. Participants did not have straight hair and did not have a permanent straightening service prior to 6 months leading up to the study start date. The hair stylist administering the treatment defined 38% of the participants as having wavy hair, 51% of the participants as having curly hair, and 10% of the participants as having coily hair.

[0182] All participants hair were deeply cleaned with OLAPLEX™ Clarifying Shampoo for up to 5 minutes to remove buildup and damaging impurities. Participants were able to follow the washing with OLAPLEX™ Chelator following the instructions on the product. 31% if the participants used the Chelator.

[0183] In step 1, hair was sectioned into 4 quadrants and the first composition as described in Table 1 was applied in small sections until hair was evenly saturated. Up to 50 ml could be used, with the amount depending on hair length and density. For short lengths, less product299472458.1 - 58 - OLAP.POOQ9WO / 1001359125was used. For long or dense lengths, more product was needed. The hair was raked through in a downward motion to ensure hair was fully saturated with product. The hair was rested with the product on the hair for 10 minutes and not rinsed.

[0184] In step 2, the hair was sprayed in small sections with the second composition described in Table 2 until the hair was thoroughly saturated. The hair was then scrunched into curls to ensure the product is properly worked through. The hair was rested for 10 minutes and not rinsed.

[0185] In step 3, starting with a small amount, the third composition described in Table 3 was evenly distributed section by section from root to tip. If needed, the hair could be misted with the second composition to re-wet the hair. The hair was then styled with the users desired curl-defining technique. The hair was allowed to air dry (preferably), or be dried by the users preferred drying method. The hair was not washed for at least 48 hours after the treatment.

[0186] The stylists were asked to rate particular attributes of applying the method steps and the products used in the method, the results are shown in Tables 4 and 5. Table 4Table 5299472458.1 - 59 - OLAP.POOQ9WO / 1001359125

[0187] The participants were asked to rate particular attributes of the results of using the method steps and the products used in the method, the results are shown in Tables 6 and 7.Table 6Table 7299472458.1 - 60 - OLAP.POOQ9WO / 1001359125

Claims

CLAIMS1. A method of treating hair, the method comprising applying a composition to the hair to form a treated hair, the composition comprising bis-aminopropyl diglycol dimaleate and guar hydroxypropyltrimonium chloride and one or more of propanediol, hydroxypropyl starch phosphate, aspergillus ferment, n Acacia senega! gum, wherein the treated hair includes at least one of the following features when compared to hair that has not been treated with the composition: (1) increased curl retention; (2) increased strength to reduce breakage of the hair; (3) tensile strength that is not decreased; (4) decreased tangling; and / or (5) reduced hair frizziness and / or hair flyaway s.

2. The method of claim 1, wherein the composition comprises 0.00001 to 1 wt. % of the bis-aminopropyl diglycol dimaleate by weight of the composition.

3. The method of any one of claims 1 to 2, wherein the composition comprises 0.05 to 2 wt. % of the guar hydroxypropyltrimonium chloride by weight of the composition.

4. The method of any one of claims 1 to 2, wherein the composition comprises propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum.

5. The method of any one of claims 1 to 2, wherein the composition comprises, by weight of the composition:0.1 to 3 wt. % of the propanediol;0.05 to 2 wt. % of the hydroxypropyl starch phosphate;0.01 to 1 wt. % of the aspergillus ferment; and0.01 to 1 wt. % of the Acacia senega! gum.

6. The method of claim 5, wherein the composition further comprises: xylitylglucoside; anhydroxylitol; hydroxy ethylcellulose; phenoxyethanol;299472458.1 - 61 - OLAP.POOQ9WO / 1001359125maltitol; glycerin; caprylyl / capryl glucoside; xylitol; polyimide- 1; and ethylhexylglycerin.

7. The method of claim 6, wherein the composition comprises, by weight of the composition:0.1 to 5 wt. % xylitylglucoside;0.1 to 3 wt. % anhydroxylitol;0.1 to 3 wt. % hydroxyethylcellulose;0.1 to 3 wt. % phenoxyethanol;0.1 to 3 wt. % maltitol;0.1 to 3 wt. % glycerin;0.05 to 2 wt. % caprylyl / capryl glucoside;0.05 to 2 wt. % xylitol;0.05 to 2 wt. % polyimide-1; and0.01 to 1 wt. % ethylhexylglycerin.

8. The method of any one of claims 1 to 2, wherein the composition remains on the treated hair for at least 2 hours, at least 6 hours, at least 12 hours, or at least 24 hours.

9. The method of any one of claims 1 to 2, wherein the hair prior to applying the composition is curly hair.

10. The method of claim 9, wherein the curly hair is naturally curly hair.

11. The method of any one of claims 1 to 2, wherein the hair prior to applying the composition is color dyed hair and / or bleached hair.299472458.1 - 62 - OLAP.POOQ9WO / 100135912512. The method of any one of claims 1 to 2, wherein increased curl retention of the hair comprises maintaining a curled shape of the hair for at least 18 hours, at least 48 hours, or at least 74 hours.

13. The method of any one of claims 1 to 2, wherein the method further comprises styling the hair within 30 minutes, within 1 hour, or within 2 hours of applying the composition to the hair.

14. The method of any one of claims 1 to 2, wherein applying the composition to the hair does not change the color of the hair once the hair is dried as determined by unaided human vision as compared to hair that has not been treated by the composition.

15. A hair care composition comprising bis-aminopropyl diglycol dimaleate and guar hydroxypropyltrimonium chloride and one or more of propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum.

16. The hair care composition of claim 15, comprising 0.00001 to 1 wt. % of the bis- aminopropyl diglycol dimaleate and / or 0.05 to 2 wt. % of the guar hydroxypropyltrimonium chloride by weight of the composition.

17. The hair care composition of any one of claims 15 to 16, comprising propanediol, hydroxypropyl starch phosphate, aspergillus ferment, and Acacia senega! gum.

18. The hair care composition of any one of claims 15 to 16, comprising, by weight of the composition:0.1 to 3 wt. % of the propanediol;0.05 to 2 wt. % of the hydroxypropyl starch phosphate;0.01 to 1 wt. % of the aspergillus ferment; and0.01 to 1 wt. % of the Acacia senega! gum.

19. The hair care composition of claim 18, further comprising: xylitylglucoside; anhydroxylitol; hydroxy ethylcellulose; phenoxyethanol;299472458.1 - 63 - OLAP.POOQ9WO / 1001359125maltitol; glycerin; caprylyl / capryl glucoside; xylitol; polyimide- 1; and ethylhexylglycerin.

20. The hair care composition of claim 19, comprising, by weight of the composition:0.1 to 5 wt. % xylitylglucoside;0.1 to 3 wt. % anhydroxylitol;0.1 to 3 wt. % hydroxyethylcellulose;0.1 to 3 wt. % phenoxyethanol;0.1 to 3 wt. % maltitol;0.1 to 3 wt. % glycerin;0.05 to 2 wt. % caprylyl / capryl glucoside;0.05 to 2 wt. % xylitol;0.05 to 2 wt. % polyimide-1; and0.01 to 1 wt. % ethylhexylglycerin.299472458.1 - 64 - OLAP.POOQ9WO / 1001359125

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