Molecular detection system and apparatus with functions of sample heating and quantitative sample addition

Abstract

The present invention relates to the technical field of nucleic acid detection and specifically to a molecular detection system and apparatus with functions of sample heating and quantitative sample addition. Provided is a molecular detection apparatus with functions of sample heating and quantitative sample addition, comprising a base, an optical acquisition system, and at least one detection module. The detection module comprises a detection cavity, a driving system, a fluid control system, and an auxiliary heating system. The detection module is arranged on the base, the detection cavity is arranged inside the detection module, and a slot into which a reagent card can be inserted is formed in the top of the detection cavity. The driving system, the fluid control system, and the auxiliary heating system are sequentially arranged in the detection cavity, and the slot is located between the fluid control system and the auxiliary heating system. The present invention effectively solves the problems of cumbersome operations, long periods of time, and large errors in the related art, enables fast sample PCR detection, requires only one sample addition step, and involves no liquid transfer step or additional manual operations.

Description

A molecular detection system and device with sample heating and quantitative sample addition functions

[0001] Cross-references to related applications

[0002] This application claims priority to Chinese Patent Application No. 202411597891.3, filed on November 11, 2024, entitled "A Molecular Detection System and Device with Sample Heating and Quantitative Sample Addition Functions", the entire contents of which are incorporated herein by reference. Technical Field

[0003] This application relates to the field of nucleic acid detection technology, specifically to a molecular detection system and device with sample heating and quantitative sample addition functions. Background Technology

[0004] Sample release agents are used to rapidly release nucleic acids (DNA / RNA) from test samples for direct amplification and detection using subsequent conventional PCR (Polymerase Chain Reaction). Using sample release agents reduces the nucleic acid extraction step, greatly simplifies the nucleic acid detection process, shortens detection time, and improves detection efficiency. Related technologies mainly include the following PCR detection methods:

[0005] The first method: Conventional PCR instrument + release reagent + manual pipette quantitative sample addition: The sample is collected and added to the sample release reagent, manually inverted for 1 second, and allowed to stand at room temperature for 5 minutes without heating. The operator uses a pipette to manually add the sample-containing release reagent to the reaction tube for quantitative analysis. This method has the following drawbacks: it takes a long time, cannot achieve rapid detection, does not involve sonication or heating, has a long pathogen lysis time, and results in incomplete nucleic acid release, affecting sensitivity.

[0006] The second method: Quantitative sample addition using a conventional PCR instrument + mini ultrasonic instrument + manual pipette. The sample-containing release agent is first treated with a mini ultrasonic instrument. Then, the operator manually adds the sample-containing release agent to the reaction tube using a pipette for quantitative analysis. This method has the disadvantages of requiring two pieces of equipment, having multiple steps, being time-consuming, and not enabling rapid detection.

[0007] It is evident that in order to accelerate the release of nucleic acid from the sample and improve the release rate, it is usually necessary to use a small ultrasonic instrument to pre-treat the sample-containing release agent, and then manually use a pipette to quantitatively add the sample. This not only makes the entire operation process cumbersome and time-consuming, but also easily introduces sampling errors. Summary of the Invention

[0008] This application provides a molecular detection device with sample heating and quantitative sample addition functions, which solves the technical problems of cumbersome and time-consuming operation procedures and easy introduction of sample addition errors in the detection process.

[0009] In a first aspect, this application provides a molecular detection device with sample heating and quantitative sample addition functions, including a base, an optical acquisition system, and at least one detection module. The detection module includes a detection chamber, a driving system, a fluid control system, and an auxiliary heating system. The detection module is mounted on the base, and a socket for inserting a reagent card is formed at the top of the detection chamber. The driving system, the fluid control system, and the auxiliary heating system are arranged sequentially within the detection chamber, with the socket located between the fluid control system and the auxiliary heating system. The fluid control system includes a main block, multiple fluid incubation driving mechanisms, and multiple fluid barrier mechanisms. The fluid incubation driving mechanisms and the fluid barrier mechanisms are arranged sequentially and spaced apart along the vertical direction on the main block. The fluid incubation drive mechanism and the fluid barrier mechanism can slide toward the auxiliary heating system. Each fluid incubation drive mechanism is equipped with a heating module for heating the reagent card. The drive system is used to drive the fluid barrier mechanism and the fluid incubation drive mechanism toward or away from the auxiliary heating system. The auxiliary heating system includes an auxiliary heating box. The end face of the auxiliary heating box facing the fluid control system has auxiliary heating areas that correspond one-to-one with the fluid incubation drive mechanism. Each auxiliary heating area is equipped with a heating module for heating the reagent card. At least one auxiliary heating area is equipped with a light-transmitting hole for optical signal transmission and reception. The optical acquisition system is used to acquire and process optical signals.

[0010] According to the above-described embodiments of the first aspect of this application, the driving system includes a plurality of first driving members and a plurality of second driving members. The first driving members correspond one-to-one with the fluid blocking mechanism and are connected to the fluid blocking mechanism, and are used to drive the fluid blocking mechanism to move closer to or away from the auxiliary heating system to isolate the liquid in the reagent card. The second driving members correspond one-to-one with the fluid incubation driving mechanism and are connected to the fluid incubation driving mechanism, and are used to drive the fluid incubation driving mechanism to move closer to or away from the auxiliary heating system to squeeze the reagent card.

[0011] According to any of the above embodiments of the first aspect of this application, at least three fluid blocking mechanisms are provided, and at least two fluid incubation driving mechanisms are provided, with the fluid incubation driving mechanism disposed between two adjacent fluid blocking mechanisms.

[0012] According to any of the above embodiments of the first aspect of this application, at least two auxiliary heating areas are provided, namely a first auxiliary heating area and a second auxiliary heating area. The two auxiliary heating areas are arranged in a vertical direction, with the first auxiliary heating area higher than the second auxiliary heating area, and a light-transmitting hole is provided in the second auxiliary heating area. The heating module in the auxiliary heating area includes at least one temperature sensor and at least one heating element or cooling element.

[0013] According to any of the above embodiments of the first aspect of this application, multiple detection modules are provided, and the multiple detection modules are arranged sequentially along the length direction of the base.

[0014] According to any of the above embodiments of the first aspect of this application, the optical acquisition system includes a scanning head module and a transmission module. The scanning head module is mounted on the transmission module, and the transmission module is used to drive the scanning head module to reciprocate along the length direction of the base to acquire the optical signals of reagent cards in multiple detection modules.

[0015] According to any of the above embodiments of the first aspect of this application, the detection module further includes a pressure cap disposed on the top of the detection cavity for sealing the insertion port.

[0016] According to any of the above embodiments of the first aspect of this application, the heating module in the fluid incubation drive mechanism includes at least one temperature sensor for temperature control and at least one heating element or cooling element.

[0017] Secondly, this application provides a molecular detection system, including a molecular detection device and a reagent card with sample heating and quantitative sample addition functions according to any of the above embodiments of the first aspect of this application. The reagent card includes a support, a tubing, and a U-shaped support plate. The top of the support is provided with an inlet for reagent entry. One end of the tubing is connected to the bottom outlet of the inlet, and the other end is connected to the inner bottom wall of the support plate. The support is provided with a plug mechanism for blocking the inlet. The two ends of the support and the U-shaped support plate are engaged. The tubing has a semi-sealed opening that opens under external pressure, and the inside of the tubing is wrapped with lyophilized beads.

[0018] According to any of the above embodiments of the second aspect of this application, the stopper mechanism includes a plastic strip and a stopper connected in sequence, the end of the plastic strip away from the stopper is connected to the side of the injection port, and the side of the stopper is provided with at least one sealing ring.

[0019] Compared to existing molecular detection devices, the molecular detection device with sample heating and quantitative sample addition functions in this application embodiment has at least the following advantages:

[0020] First, it eliminates the need for an ultrasound machine, using a fluid control system and an auxiliary heating system to heat the reagent samples used for amplification, eliminating the need for manual operation and reducing human error. It reduces the number of steps and the time required to achieve the same results.

[0021] Secondly, the sample is added quantitatively to the reaction solution, eliminating the need for a pipette and avoiding the inaccuracies of manual pipetting, thus ensuring the repeatability and reliability of each experiment. Precise sample volume control and pre-programmed procedures guarantee the accuracy and consistency of each sample addition.

[0022] Third, the integrated device combines functions such as heating, quantitative sample addition, and amplification, simplifying the operation process and making it suitable for POCT nucleic acid testing needs. The integrated design reduces switching and operational steps between different devices, improving overall operational efficiency.

[0023] Fourth, the sample volume is controlled by squeezing, and then amplified after being mixed with amplification reagents, so that the entire PCR operation and reaction time can be completed within 10 minutes, greatly improving the detection efficiency.

[0024] This application effectively solves the problems of cumbersome operation, long time and large error in related technologies through innovative sample processing methods and integrated design. It can quickly complete sample PCR detection with only one sample addition step, without any pipetting steps or additional manual operations. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the specific embodiments or related technologies of this application, the drawings used in the description of the specific embodiments or related technologies will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0026] Figure 1 is a structural diagram of the reagent card provided in an embodiment of this application;

[0027] Figure 2 is a structural diagram of the nucleic acid detection instrument provided in an embodiment of this application;

[0028] Figure 3 is a structural diagram of the detection module provided in an embodiment of this application;

[0029] Figure 4 is a front view of the fluid control system provided in an embodiment of this application;

[0030] Figure 5 is a front view of the auxiliary heating system provided in an embodiment of this application;

[0031] Figure 6 is a structural diagram of the optical acquisition system provided in an embodiment of this application;

[0032] Figure 7 is a schematic diagram of the optical orientation of the scanning head module provided in an embodiment of this application;

[0033] Figure 8 shows the positional relationship between the reagent card, fluid control system, and auxiliary heating system provided in the embodiments of this application during sample quantification and sample heating.

[0034] Explanation of reference numerals in the attached diagram: 1. Reagent card; 11. Support; 111. Inlet; 112. Plug; 1121. Sealing ring; 113. Snap-fit; 12. Tube; 121. Bottom seal; 122. Semi-seal; 123. Lyophilized beads; 13. Support plate; 131. Slot; 2. Nucleic acid detector; 21. Detection module; 211. Drive system; 212. Fluid control system; 2121. Fluid incubation drive mechanism; 21211. First fluid incubation drive mechanism; 21212. Second fluid incubation drive mechanism; 2122. Fluid barrier mechanism; 21221. First fluid barrier mechanism; 21222. Second fluid barrier mechanism; 21223. 213. Third fluid barrier mechanism; 214. Auxiliary heating system; 2131. Light-transmitting hole; 2132. Auxiliary heating area; 21321. First auxiliary heating area; 21322. Second auxiliary heating area; 215. Pressure cap; 216. Single module control circuit; 22. Optical acquisition system; 221. Scanning head module; 2211. Excitation light source; 2212. Excitation light lens; 2213. Laser light filter; 2214. Dichroic mirror; 2215. Emitting lens; 2216. Emitting filter; 2217. Fluorescence collector; 222. Conveying module; 23. Control system; 24. Base; a. Required volume sample; b. Excess volume sample. Detailed Implementation

[0035] The technical solutions of this application will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0036] Example 1:

[0037] As shown in Figures 2-8, Embodiment 1 of this application provides a molecular detection device with sample heating and quantitative sample addition functions, specifically a nucleic acid detector 2, which includes a base 24, an optical acquisition system 22, and at least one detection module 21. The detection module 21 includes a detection chamber, a drive system 211, a fluid control system 212, and an auxiliary heating system 213. The detection module is mounted on the base 24, and a socket for inserting a reagent card 1 is formed at the top of the detection chamber. The drive system 211, the fluid control system 212, and the auxiliary heating system 213 are arranged sequentially within the detection chamber, with the socket located between the fluid control system 212 and the auxiliary heating system 213. The fluid control system 212 includes a main block, multiple fluid incubation drive mechanisms 2121, and multiple fluid barrier mechanisms 2122. The fluid incubation drive mechanisms 2121 and the fluid barrier mechanisms 2122 are arranged sequentially and spaced apart along the vertical direction on the main block. The fluid incubation drive mechanism 2121 and the fluid barrier mechanism 2122 are slidable toward the auxiliary heating system 213. Each fluid incubation drive mechanism 2121 is equipped with a heating module for heating the reagent card 1. The drive system 211 is used to drive the fluid barrier mechanism 2122 and the fluid incubation drive mechanism 2121 toward or away from the auxiliary heating system 213. The auxiliary heating system 213 includes an auxiliary heating housing. On the end face of the auxiliary heating housing facing the fluid control system 212, auxiliary heating areas 2132 are formed, each corresponding to one of the fluid incubation drive mechanisms 2121. Each auxiliary heating area 2132 is equipped with a heating module for heating the reagent card 1. At least one auxiliary heating area 2132 is equipped with a light-transmitting hole 2131 for optical signal transmission and reception. The optical acquisition system 22 is used to acquire and process optical signals.

[0038] The reagent card 1 used in this embodiment, as shown in Figure 1, includes a support 11, a tubing 12, and a U-shaped support plate 13. The support 11 has a flow channel extending through it on its upper surface, and an inlet 111 connected to the flow channel is located on its upper surface. The top of the tubing 12 is open and connected to the flow channel of the support 11. The bottom end of the tubing 12 has a bottom seal 121, which is connected to the inner bottom wall of the support plate 13. As shown in Figure 8, a lyophilized bead 123 is wrapped inside the bottom end of the tubing 12, and a semi-sealed opening 122 is provided inside. This semi-sealed opening 122 can be opened under external pressure without damaging the tubing 12. Furthermore, a plug mechanism for blocking the inlet 111 is provided on the support 11.

[0039] When using this molecular detection device with sample heating and quantitative sample addition functions for amplification experiments, first dilute PIV1 with PBS (polybutylene succinate), take a certain amount of diluent and add it to the extraction bottle containing the release agent, mix well, then drop a fixed amount of diluent into the injection port 111 of the reagent card 1 containing lyophilized beads 123 and a semi-sealed cap 122, and then close the stopper mechanism. Next, reagent card 1 is inserted into the socket of the detection chamber. Then, the drive system 211 drives different fluid incubation drive mechanisms 2121 and fluid barrier mechanism 2122 to isolate the excess sample in the tubing 12 to the top of the tubing 12, away from the temperature control area, thus completing the sample quantification. Then, the heating module in the fluid incubation drive mechanism 2121 and the heating module in the auxiliary heating area 2132 work together to preheat the quantified sample, improving the release efficiency of nucleic acid in the sample. Then, the drive system 211 drives the fluid incubation drive mechanism 2121 to squeeze the heated sample into the area of ​​the lyophilized beads 123 below, and squeezes it up and down to mix it evenly with the lyophilized beads 123. During this process, the optical acquisition system 22 continuously acquires optical signals through the light-transmitting hole 2131, collects multiple sets of fluorescence data, and obtains the amplification curve results.

[0040] Compared to existing molecular detection devices, the molecular detection device with sample heating and quantitative sample addition functions in this application embodiment has at least the following advantages:

[0041] First, it eliminates the need for an ultrasonic instrument. The reagent sample used for amplification is heated through a fluid control system 212 and an auxiliary heating system 213, eliminating the need for manual operation and reducing human error. This reduces the number of steps and the time required to achieve the same effect.

[0042] Secondly, the sample is added quantitatively to the reaction solution, eliminating the need for a pipette and avoiding the inaccuracies of manual pipetting, thus ensuring the repeatability and reliability of each experiment. Precise sample volume control and pre-programmed procedures guarantee the accuracy and consistency of each sample addition.

[0043] Third, the integrated device combines functions such as heating, quantitative sample addition, and amplification, simplifying the operation process and making it suitable for POCT nucleic acid testing needs. The integrated design reduces switching and operational steps between different devices, improving overall operational efficiency.

[0044] Fourth, the sample volume is controlled by squeezing, and then amplified after being mixed with amplification reagents, so that the entire PCR operation and reaction time can be completed within 10 minutes, greatly improving the detection efficiency.

[0045] In summary, this application effectively solves the problems of cumbersome operation, long time and large error in related technologies through innovative sample processing methods and integrated design. It can quickly complete sample PCR detection with only one sample addition step, without any pipetting steps or additional manual operations.

[0046] PIV1 is human parainfluenza virus type 1, belonging to the Paramyxoviridae family, and is a single-stranded RNA virus.

[0047] As shown in Figure 3, in a specific embodiment, the detection module is mounted on the base 24. The drive system 211, the fluid control system 212, and the auxiliary heating system 213 are arranged sequentially within the detection chamber, with the insertion port located between the fluid control system 212 and the auxiliary heating system 213. The fluid incubation drive mechanism 2121 in the fluid control system 212 is plate-shaped and consists of two units. For easy differentiation, they are named, from highest to lowest, the first fluid incubation drive mechanism 21211 and the second fluid incubation drive mechanism 21212. The fluid control system 212 contains three fluid blocking mechanisms 2122, which are block-shaped and are named, from highest to lowest, the first fluid blocking mechanism 21221, the second fluid blocking mechanism 21222, and the third fluid blocking mechanism 21223. Furthermore, the first fluid incubation drive mechanism 21211 is located between the first fluid barrier mechanism 21221 and the second fluid barrier mechanism 21222, and the second fluid incubation drive mechanism 21212 is located between the second fluid barrier mechanism 21222 and the third fluid barrier mechanism 21223.

[0048] The first fluid incubation drive mechanism 21211, the second fluid incubation drive mechanism 21212, the first fluid barrier mechanism 21221, the second fluid barrier mechanism 21222, and the third fluid barrier mechanism 21223 are slidably mounted on the block of the fluid control system 212. Further, the drive system 211 includes three first drive members and two second drive members. Each of the three first drive members corresponds to one of the three fluid barrier mechanisms 2122. Each first drive member is connected to its corresponding fluid barrier mechanism 2122 and is used to drive the fluid barrier mechanism 2122 towards or away from the auxiliary heating system 213 to isolate the sample in the reagent card 1. Each of the two second drive members corresponds to one of the two fluid incubation drive mechanisms 2121. Each second drive member is connected to its corresponding fluid incubation drive mechanism 2121 and is used to drive the fluid incubation drive mechanism 2121 towards or away from the auxiliary heating system 213 to squeeze the reagent card 1.

[0049] Correspondingly, as shown in Figure 5, auxiliary heating areas 2132 are formed on the end face of the auxiliary heating box facing the fluid control system 212, corresponding one-to-one with the fluid incubation drive mechanism 2121; that is, there are two auxiliary heating areas 2132. In descending order of height, the two auxiliary heating areas 2132 are named the first auxiliary heating area 21321 and the second auxiliary heating area 21322, respectively. Each auxiliary heating area 2132 is equipped with a heating module for preheating the reagent card 1.

[0050] Specifically, as shown in Figure 8, PIV1 is first diluted 1000 times with PBS, and 100 μL is added to an extraction bottle containing the release agent. After mixing, 2 drops (approximately 50 μL) are added to reagent card 1 containing lyophilized beads 123 and a semi-sealed end 122, and the stopper mechanism is fastened. Then, reagent card 1 is inserted into the socket of the detection chamber. According to the program execution steps, the detection module 21 first moves to the left under the drive of the first driving component to press the semi-sealed end 122 of the tubing 12. At the same time, the first fluid incubation driving mechanism 21211 above moves a set distance under the drive of the second driving component to squeeze the sample in the tubing 12. The first fluid barrier mechanism 21221 at the top presses the tubing 12 to the left. At this time, the excess volume of sample b, approximately 30 μL, is isolated above the tubing 12 and removed from the temperature control area. The required volume of sample a, approximately 20 μL, is quantified.

[0051] The heating modules in the first fluid incubation drive mechanism 21211 and the first auxiliary heating area 21321 heat the required volume of sample a to 95°C and maintain this temperature for 1 minute according to a set program, thereby improving the release efficiency of nucleic acid in the sample. Then, the second fluid barrier mechanism 21222 retracts a set distance, and the first fluid incubation drive mechanism 21211 squeezes the tubing 12 again to squeeze the required volume of sample a into the lower freeze-drying bead 123 area. Next, the heating modules in the second fluid incubation drive mechanism 21212 and the second auxiliary heating area 21322 heat the required volume of sample a to 55°C and maintain this temperature for 2 seconds according to a program. Subsequently, the second fluid incubation drive mechanism 21212 squeezes the required volume of sample a into the upper area. This up-and-down squeezing process ensures that the required volume of sample a is evenly mixed with the freeze-drying bead 123, until the cycle is repeated 40 times.

[0052] After each volume sample a is squeezed into the area where the freeze-dried bead 123 is located and remains there for 2 seconds, the optical acquisition system 22 acquires the optical signal through the light-transmitting hole 2131. Forty sets of fluorescence data are collected, and the amplification curve results are obtained.

[0053] In this embodiment, since the first fluid incubation drive mechanism 21211 moves a set distance each time under the drive of the second drive component, the squeezing force applied to the tubing 12 by the first fluid incubation drive mechanism 21211 is fixed each time, so the excess volume of sample b discharged each time is approximately 30 μL. This achieves the function of quantitatively retaining the remaining sample, avoids the inaccuracy of manual pipetting, and ensures the repeatability and reliability of each experiment.

[0054] Furthermore, since the heating modules in the first fluid incubation drive mechanism 21211, the second fluid incubation drive mechanism 21212, the first auxiliary heating area 21321, and the second auxiliary heating area 21322 preheat the sample according to the set program, there is no need to use an ultrasonic heater. Moreover, the entire preheating process is controlled by the program, eliminating the need for manual operation and reducing human error.

[0055] As shown in Figures 5 and 8, the light-transmitting hole 2131 is disposed on the second auxiliary heating area 21322.

[0056] In a preferred embodiment, the heating module in each fluid incubation drive mechanism 2121 includes a temperature sensor, a heating element, and a cooling element for temperature control. Each detection module 21 is equipped with a single-module control circuit 215. The temperature sensor, heating element, and cooling element in the fluid incubation drive mechanism 2121 are wired to the single-module control circuit 215. An embedded program controls the temperature sensor, heating element, and cooling element to achieve preheating of the reagent card 1.

[0057] Furthermore, each auxiliary heating zone 2132 includes a heating module comprising a temperature sensor, a heating element, and a cooling element. The temperature sensor, heating element, and cooling element in the auxiliary heating zone 2132 are each wired to a single-module control circuit 215, and are controlled by an embedded program.

[0058] In a preferred embodiment, the three first driving elements and the two second driving elements are all miniature electric actuators. The miniature electric actuators are mounted on the block of the fluid control system 212, and these multiple miniature electric actuators are respectively connected to the single module control circuit 215 for signal connection. The single module control circuit 215 drives the three first driving elements and the two second driving elements to work.

[0059] Furthermore, as shown in Figure 3, a top plate is installed at the top opening of each detection chamber, and an insertion port is opened on the top plate. After the reagent card 1 is inserted into the insertion port of the top plate, the bracket 11 of the reagent card 1 rests on the insertion port. In addition, a pressure cap 214 is hinged to the end of the top plate. The pressure cap 214 is equipped with a push-type lock, which is used to press the top of the reagent card 1 after the reagent card 1 is inserted, thereby better fixing the reagent card 1.

[0060] In this embodiment, as shown in Figure 2, multiple detection modules 21 are arranged sequentially along the length of the base 24. The optical acquisition system 22 is located on the back of the detection modules 21, near the pressure cap 214. The optical acquisition system 22 includes a scanning head module 221 and a transmission module 222. The scanning head module 221 is mounted on the transmission module 222, which drives the scanning head module 221 to reciprocate along the length of the base 24 to acquire the optical signals of the reagent cards 1 within the multiple detection modules 21.

[0061] Furthermore, as shown in Figure 6, the conveyor module 222 adopts a belt conveyor mechanism, and the scanning head module 221 is mounted on the belt of the belt conveyor mechanism. As the belt rotates, it moves back and forth along the arrangement direction of the scanning head module 221. The belt drives the scanning head module 221 to move laterally back and forth within a set range, thereby completing the optical scanning action.

[0062] In addition, the optical acquisition system 22 may also include a guide rail, which is set along the length of the base 24, and the scanning head module 221 is slidably connected to the guide rail to improve the stability of the scanning head module 221 when it moves.

[0063] Preferably, as shown in Figure 7, the scanning head module 221 contains excitation and emission elements for multiple fluorescence detection channels.

[0064] In some embodiments, each fluorescence detection channel comprises at least an excitation light source 2211, an excitation light lens 2212, a laser light filter 2213, a dichroic mirror 2214, an emission lens 2215, an emission filter 2216, and a fluorescence collector 2217.

[0065] Optionally, each fluorescence detection channel can also be designed to include optical components such as collimating lenses and light guides.

[0066] It should be noted that the number of fluorescence detection channels in this embodiment is ≥2. Specifically, the number of fluorescence detection channels can be 4, namely FAM / VIC / ROX / Cy5 channels.

[0067] Example 2:

[0068] This embodiment 2 provides a molecular detection system, including a molecular detection device with sample heating and quantitative sample addition functions as described in embodiment 1 and a reagent card 1, as shown in Figure 1. The reagent card 1 includes a support 11, a flexible tube 12 and a U-shaped support plate 13. The upper surface of the support 11 has a flow channel that passes through the support 11, and the upper surface of the support 11 has an inlet 111 that communicates with the flow channel. The top of the flexible tube 12 is open and communicates with the flow channel of the support 11. The bottom end of the flexible tube 12 has a bottom seal 121, and the bottom seal 121 of the bottom end of the flexible tube 12 is connected to the inner bottom wall of the support plate 13.

[0069] The two ends of the bracket 11 and the U-shaped support plate 13 are interlocked; the hose 12 is provided with a semi-sealed opening 122 that can be opened under external pressure, the hose 12 is wrapped with freeze-dried beads 123, and the bracket 11 is provided with a plug mechanism for blocking the injection port 111.

[0070] Preferably, the scaffold 11 is made of a polymer material, for example, it can be any one of PTFE, PA, PC, PET, PP, PMMA, and its color can be transparent or translucent.

[0071] The support plate 13 is made of a polymer material, for example, it can be any one of PTFE, PA, PC, PET, PP, and PMMA.

[0072] For example, the thickness H of the support plate 13 can be in the range of 0.1mm ≤ H ≤ 3mm.

[0073] Preferably, the flexible hose 12 is a flexible film with a certain degree of elasticity and extensibility, and its color can be transparent or semi-transparent. The top end of the flexible hose 12 is tightly connected to the support 11, and the connection method can be adhesive bonding, welding, injection molding, etc. There is a bottom seal 121 at the bottom to prevent leakage of internal substances. The bottom seal 121 is connected to the bottom inner wall of the support plate 13, and the connection method can be heat sealing, welding, adhesive bonding, etc.

[0074] For example, the wall thickness h of the hose 12 is in the range of 0.01mm≤h≤1mm; its length L is in the range of 2mm≤L≤200mm; and its inner diameter d is in the range of 1mm≤d≤8mm.

[0075] In a preferred embodiment, the stopper mechanism includes a plastic strip and a stopper 112 connected in sequence. The end of the plastic strip away from the stopper 112 is connected to the side of the injection port 111. The side of the stopper 112 is provided with at least one sealing ring 1121. The sealing ring 1121 cooperates with the stopper 112 to seal the injection port 111, thereby ensuring airtightness and preventing the evaporation of reagents from causing environmental pollution.

[0076] It should be noted that the connection between the support plate 13 and the bracket 11 can be achieved through welding, hot pressing, gluing, snap-fitting, etc. Snap-fitting is preferred. For example, the bracket 11 has hook-shaped snap-fits 113 for connecting to the support plate 13, and two snap-fits 113 are provided, located on both sides of the bottom of the bracket 11. The top two ends of the support plate 13 are respectively provided with slots 131 for engaging with the snap-fits 113.

[0077] Furthermore, as shown in Figure 2, this molecular detection system also includes a control system 23, which is located in the space below the base 24. The control system 23 includes a main control circuit and a corresponding program. The control system 23 is used to trigger relevant reaction programs, including but not limited to temperature control, motion control, optical detection, and data analysis, after the reagent card 1 is inserted into the instrument, thereby completing the experiment.

[0078] This molecular detection system also includes an outer casing (not shown in the figure), which serves for the external encapsulation, protection, and aesthetic purposes of the instrument.

[0079] This molecular detection system allows for PCR testing of samples within 10 minutes. It requires only one sample loading step, eliminating any pipetting or additional manual operations. The same sample can be amplified up to 10 times with a CV value <3%, avoiding errors caused by pipetting. Therefore, this application, combined with a sample release agent, effectively reduces PCR testing time from 60-90 minutes to less than 10 minutes. The entire process requires only one sample loading step, eliminating cumbersome operations. This improves PCR detection efficiency, reduces operator workload, and minimizes deviations in experimental results due to operator error.

[0080] In the description of this application, it should be noted that the terms "upper" and "lower," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0081] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linkage" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances. Furthermore, in the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0082] The above description is only a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A molecular detection device with sample heating and quantitative sample addition functions, comprising a base (24), an optical acquisition system (22) and at least one detection module (21), wherein the detection module (21) comprises a detection cavity, a driving system (211), a fluid control system (212) and an auxiliary heating system (213); The detection module is mounted on the base (24), and the top of the detection cavity has a socket for inserting a reagent card (1); the drive system (211), the fluid control system (212) and the auxiliary heating system (213) are arranged sequentially in the detection cavity, and the socket is located between the fluid control system (212) and the auxiliary heating system (213); The fluid control system (212) includes a block, multiple fluid incubation drive mechanisms (2121), and multiple fluid blocking mechanisms (2122). The fluid incubation drive mechanisms (2121) and the fluid blocking mechanisms (2122) are arranged in a vertical direction at intervals on the block. The fluid incubation drive mechanisms (2121) and the fluid blocking mechanisms (2122) can slide toward the auxiliary heating system (213). Each fluid incubation drive mechanism (2121) is provided with a heating module for heating the reagent card (1). The driving system (211) is used to drive the fluid barrier mechanism (2122) and the fluid incubation driving mechanism (2121) to move closer to or away from the auxiliary heating system (213); the auxiliary heating system (213) includes an auxiliary heating box, and the auxiliary heating box has auxiliary heating areas (2132) corresponding one-to-one with the fluid incubation driving mechanism (2121) on the end face of the auxiliary heating box facing the fluid control system (212). Each auxiliary heating area (2132) is provided with a heating module for heating the reagent card (1); at least one of the auxiliary heating areas (2132) is provided with a light-transmitting hole (2131) for optical signal transmission and reception. The optical acquisition system (22) is used to acquire and process optical signals.

2. The molecular detection device with sample heating and quantitative sample addition functions according to claim 1, wherein, The driving system (211) includes a plurality of first driving elements and a plurality of second driving elements. The first driving elements and the fluid blocking mechanism (2122) correspond one-to-one. The first driving elements and the fluid blocking mechanism (2122) are connected to each other and are used to drive the fluid blocking mechanism (2122) to move closer to or away from the auxiliary heating system (213) to isolate the liquid in the reagent card (1). The second driving member and the fluid incubation driving mechanism (2121) correspond one-to-one. The second driving member and the fluid incubation driving mechanism (2121) are connected to each other and are used to drive the fluid incubation driving mechanism (2121) to move closer to or away from the auxiliary heating system (213) to squeeze the reagent card (1).

3. A molecular detection device with sample heating and quantitative sample addition functions according to claim 2, wherein, At least three fluid barrier mechanisms (2122) are provided, and at least two fluid incubation drive mechanisms (2121) are provided. The fluid incubation drive mechanism (2121) is provided between two adjacent fluid barrier mechanisms (2122).

4. A molecular detection device with sample heating and quantitative sample addition functions according to claim 3, wherein, At least two auxiliary heating areas (2132) are provided, namely a first auxiliary heating area and a second auxiliary heating area. The two auxiliary heating areas (2132) are arranged in a vertical direction. The first auxiliary heating area is higher than the second auxiliary heating area. The light-transmitting hole (2131) is provided in the second auxiliary heating area. The heating module in the auxiliary heating area (2132) includes at least one temperature sensor and at least one heating element or cooling element.

5. A molecular detection device with sample heating and quantitative sample addition functions according to claim 4, wherein, Multiple detection modules (21) are provided, and the multiple detection modules (21) are arranged sequentially along the length direction of the base (24).

6. A molecular detection device with sample heating and quantitative sample addition functions according to claim 5, wherein, The optical acquisition system (22) includes a scanning head module (221) and a transmission module (222). The scanning head module (221) is mounted on the transmission module (222). The transmission module (222) is used to drive the scanning head module (221) to reciprocate along the length direction of the base (24) to acquire the optical signals of the reagent cards (1) in the multiple detection modules (21).

7. A molecular detection device with sample heating and quantitative sample addition functions according to claim 1, wherein, The detection module (21) also includes a pressure cap (214), which is located on the top of the detection cavity and is used to seal the insertion port.

8. A molecular detection device with sample heating and quantitative sample addition functions according to claim 1, wherein, The heating module in the fluid incubation drive mechanism (2121) includes at least one temperature sensor for temperature control and at least one heating element or cooling element.

9. A molecular detection system, comprising a molecular detection device with sample heating and quantitative sample addition function as described in any one of claims 1-8 and a reagent card (1), wherein the reagent card (1) comprises a support (11), a tubing (12) and a U-shaped support plate (13), wherein the top of the support (11) is provided with an inlet (111) for reagent entry, one end of the tubing (12) is connected to the bottom outlet of the inlet (111), and the other end is connected to the inner bottom wall of the support plate (13); the support (11) is provided with a plug mechanism for blocking the inlet (111), and the two ends of the support (11) and the U-shaped support plate (13) are engaged; the tubing (12) is provided with a semi-sealed opening (122) that opens under external pressure, and the tubing (12) is filled with lyophilized beads.

10. A molecular detection system according to claim 9, wherein, The plug mechanism includes a plastic strip and a plug (112) connected in sequence. One end of the plastic strip away from the plug (112) is connected to the side of the injection port (111). The side of the plug (112) is provided with at least one sealing ring (1121).

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