Composition comprising mono-rhamnolipid in solution

Abstract

The invention relates to a composition comprising at least one mono-rhamnolipid in solution at very high concentrations, specific organic acid and which has a specific acidic pH, a method to its production and its use.

Description

[0001] 201400149 Foreign Filing 1

[0002] Composition comprising mono-rhamnolipid in solution

[0003] Field of the invention

[0004] The invention relates to a composition comprising at least one mono-rhamnolipid in solution at very high concentrations, a specific organic acid and which has a specific acidic pH, a method to its production and its use.

[0005] Prior art

[0006] Rhamnolipids are surface active Glycolipids and metabolites of particular microorganisms. They have exceptional properties as surfactants as for example, strong foam formation and are of highest interest for a wide variety of technical applications.

[0007] Surfactants, that are mainly being used in cosmetic formulations (e.g. Shampoos, liquid soaps), household detergents as well as dishwashing detergents, have to be liquid at commonly used processing temperatures of the products’ manufacturers, to maintain and enable their usage in the manufacturers’ respective pipeline systems and pumps. Therefore, the raw material’s viscosity should be rather low to maintain a simple and continuous delivery. At the same time, the highest possible surfactant concentration is desired to allow preparation of environmentally-friendly formulations having a low water content.

[0008] W02024068270 discloses a composition comprising a) at least one sophorolipid b) lactic acid and / or a salt thereof, characterized in that a) is comprised in an amount of from 1 .2 wt.-% to 70.0 wt.-% and b) is comprised in an amount of from 0.08 wt.-% to 10.0 wt.-%, wherein the weight percentages refer to the total composition.

[0009] EP4206148 discloses an aqueous formulation comprising at least one surfactant of microbial origin (so-called biosurfactant) selected from glycolipids, lipopeptides, lipoproteins, phospholipids and polymeric biosurfactants, preferably from the group consisting of sophorolipids, rhamnolipids, trehalolipids, cellobiose lipids, surfactin, viscosin, lichenysin, iturin, fengycin, emulsan, biodispersan, alasan, liposan, at least one organic acid selected from citric acid, lactic acid, acetic acid, malic acid, maleic acid, formic acid, caprylic acid, methanesulfonic acid, tartaric acid, succinic acid, and polyaspartic acid, wherein the weight ratio between said at least one surfactant and said at least one organic acid is between 1 :1000 and 10:1. 201400149 Foreign Filing 2

[0010] WO2022046899 a cleaning composition, comprising at least one pH adjusting agent; and at least one biosurfactant selected from the group consisting of glycolipids, lipopeptides, and mixtures thereof; wherein the composition has a pH of from about 1 to about 14.

[0011] So far, no composition is available that allows for very high concentrations of rhamnolipids still being liquid and therefore, being useful as a stock solution for the preparation of end consumer formulations.

[0012] It is an object of the invention to provide rhamnolipid solutions with exceptional high concentrations of rhamnolipids at less acidic pH values that can be found in literature.

[0013] Description of the invention

[0014] It was found that, surprisingly, compositions according to claim 1 solve the above problem.

[0015] The present invention therefore provides a composition comprising at least one mono-rhamnolipid in solution and at least one organic acid and / or its salt selected from the group of benzoic acid, caprylhydroxamic acid, cinnamic acid citric acid, dehydroacetic acid, geranic acid, lactic acid, levulinic acid, mandelic acid, p-anisic acid, pelargonic acid, salicylic acid, sorbic acid, succinic acid, wherein the total content of said mono-rhamnolipid in solution is from 60.0 wt.-% to 80.0 wt.-%, and wherein the content of said organic acid is from 0.1% wt.-% to 10.0 wt.-%, wherein the weight percentages refer to the total composition, characterized in that the pH of the composition at 25 °C is in the range of 2.5 to 5.5.

[0016] The invention further provides a process of producing a composition comprising at least one mono- rhamnolipid in solution.

[0017] An advantage of the present invention is that the pH value of the composition can be less acidic compared to the raw material itself.

[0018] An advantage of the present invention is that product is easy to handle at less acidic pH Values compared to the raw material itself.

[0019] Another advantage of the composition according to the invention is its low viscosity and therefore simple processability in any desired aqueous surface-active system.

[0020] An advantage of the present invention is the reduced necessity for microbial active preservatives. An advantage of the present invention is that with a higher pH value lower amounts of bases are needed in the final formulation. 201400149 Foreign Filing 3

[0021] A further advantage of the compositions according to the invention is their good skin and hair cleansing properties.

[0022] A further advantage of the compositions according to the invention is their good lipstick removal property.

[0023] A further advantage of the compositions according to the invention is their good eye make-up removal property.

[0024] A further advantage of the compositions according to the invention is their very good solubilizing efficacy active ingredients at low usage levels.

[0025] A further advantage of the compositions according to the invention is their high compatibility with active ingredients.

[0026] A further advantage of the mixture compositions according to the invention is that they can be synthesized free from petrochemical-based raw materials.

[0027] A further advantage of the mixture compositions according to the invention is their outstanding microbiological stability.

[0028] A further advantage of the mixture compositions according to the invention is that they can be clear and non-turbid.

[0029] A further advantage of the composition according to the invention is their mildness and good physiological compatibility.

[0030] A further advantage of the compositions according to the invention is their good skin feel during and after washing.

[0031] A further advantage of the compositions according to the invention is their good rinseability compared to other surface-active compositions.

[0032] A further advantage of the compositions according to the invention is that they leave a smooth and soft skin feel after washing.

[0033] A further advantage of the mixture compositions according to the invention is that active ingredients can be incorporate into cold process formulations.

[0034] A further advantage of the mixture compositions according to the invention that no heating of the formulation is needed to incorporate active ingredients.

[0035] A further advantage of the mixture compositions according to the invention is that active ingredients can be easily solubilized resulting in a clear formulation.

[0036] A further advantage of the mixture compositions according to the invention is that active ingredients can be easily solubilized at lower temperatures resulting in a clear formulation.

[0037] A further advantage of the mixture compositions according to the invention is the water solubility of the composition.

[0038] A further advantage is that L-lactic acid enantiomer can be comprised, which can be produced solely obtained by fermentation and therefore is a sustainably and natural raw material.

[0039] Another advantage of the invention is, that the ionic strength of the rhamnolipid-based composition can be kept low. Therefore, the formulator can have a high flexibility in the adjustment of the pH value and salt content in the final formulation. 201400149 Foreign Filing 4

[0040] Another advantage of the invention is, that the composition has an increased microbiological stability.

[0041] Another advantage of the invention is, that the composition can be readily diluted, as the rhamnolipid as active matter already is in its dissolved state.

[0042] Another advantage of the invention is, that the composition can be thoroughly mixed with other surfactants.

[0043] Another advantage of the invention is, that the composition enables the formulation of concentrated end formulations (‘concentrates’).

[0044] Another advantage of the invention is, that the composition has a reduced foam tendency on account of their high concentrations and thus transport and conveying are simplified.

[0045] Another advantage of the invention is, that the composition enables easy incorporation of hydrophobic components such as oils, for example.

[0046] Another advantage of the invention is, that the compositions have a high storage stability.

[0047] Another advantage of the invention is, that when diluted with water no significant changes in viscosity can be observed.

[0048] Another advantage of the invention is, that the composition causes less contamination in pipelines during preparation and transport thereof, and therefore, enable simpler cleaning.

[0049] Another advantage of the invention is, that the composition causes a much lower energy consumption for its transportation and therefore, has a lower CO2 footprint.

[0050] Another advantage of the invention is, that it shows a lower water content and therefore hydrophobic raw materials, e.g., essential oils, can be solubilized and / or incorporated more easily. Another advantage of the invention is, that the composition can solubilize ceramide-based actives more efficiently if not at all.

[0051] Another advantage of the invention is, that the composition shows a lower foaming volume in a water-based solutions compared to di-Rhamnolipids.

[0052] Another advantage of the invention is, that the composition shows a higher colour stability compared to di-Rhamnolipids.

[0053] Another advantage of the invention is, that the composition shows reduced odour compared to dirhamnolipids.

[0054] Another advantage of the invention is, that the composition shows reduced odour compared to sophorolipids.

[0055] Another advantage of the invention is, that incorporating the composition in a formulation reduces the need of an acid or reduced the amount needed to adjust the pH accordingly.

[0056] Another advantage of the invention is, that the composition can have very low viscosities, and thus can be handled easily, e.g., via pumps.

[0057] The composition according to the instant invention comprises at least one mono-rhamnolipid in solution and at least one organic acid and / or its salt selected from the group of benzoic acid, caprylhydroxamic acid, cinnamic acid, citric acid, dehydroacetic acid, geranic acid, lactic acid, levulinic acid, mandelic acid, p-anisic acid, pelargonic acid, salicylic acid, sorbic acid, succinic acid, 201400149 Foreign Filing 5 preferably lactic acid, most preferably L-(+)-lactic acid, wherein the total content of said monorhamnolipid in solution is from 60.0 wt.-% to 80.0 wt.-%, preferably from 65.0 wt.-% to 76.0 wt.-%, more preferably from 68.0 wt.-% to 74.0 wt.-%, and wherein the content of said organic acid is from 0.1% wt.-% to 10.0 wt.-%, preferably from 1 .0 wt.-% to 7.0 wt.-%, more preferably from 2.0 wt.-% to 5.0 wt.-%, wherein the weight percentages refer to the total composition, characterized in that the pH of the composition at 25 °C is in the range of 2.5 to 5.5, preferably 2.7 to 5.2, more preferably 3.0 to 4.9, most preferably 3.3 to 4.5.

[0058] Where average values are stated hereinbelow, then, unless stated otherwise, these are number- averaged average values.

[0059] Unless stated otherwise, percentages are data in percent by weight. The same is true for parts per million (ppm).

[0060] Wherever measurement values are stated hereinbelow, then, unless stated otherwise, these have been determined at a temperature of 25°C and a pressure of 1013 mbar.

[0061] When determining the content of rhamnolipids in the context of the present invention, the mass of the non-salt form is taken into account; thus, the weight of the corresponding cation is disregarded. The same is true for the content of said organic acid, when comprised in the composition according to the instant invention as a salt.

[0062] The term "rhamnolipids" in the context of the present invention preferably is understood to mean particularly compounds of the general formula (I) and salts thereof, Formula (I) where 201400149 Foreign Filing 6 mRL = 2, 1 or 0, nRL = 1 or 0,

[0063] R1RLand R2RL= mutually independently, identical or different, organic residues having 2 to 24, preferably 5 to 13 carbon atoms, in particular optionally branched, optionally substituted, particularly hydroxy-substituted, optionally unsaturated, in particular optionally mono-, bi- or triunsaturated alkyl residues, preferably those selected from the group consisting of pentenyl, heptenyl, nonenyl, undecenyl and tridecenyl and (CH2)o-CH3 where o = 1 to 23, preferably 4 to 12. If nRL = 1 , the glycosidic bond between the two rhamnose units is preferably in the a-configuration. The optically active carbon atoms of the fatty acids are preferably present as R-enantiomers (e.g. (R)-3-{(R)-3-[2-O-(a-L-rhamnopyranosyl)-a-L-rhamnopyranosyl]oxydecanoyl}oxydecanoate).

[0064] The term "di-rhamnolipid" in the context of the present invention is understood to mean compounds of the general formula (I) or salts thereof, where nRL = 1 .

[0065] The term "mono-rhamnolipid" in the context of the present invention is understood to mean compounds of the general formula (I) or salts thereof, where nRL = 0.

[0066] Distinct rhamnolipids are abbreviated according to the following nomenclature: "diRL-CXCY" are understood to mean di-rhamnolipids of the general formula (I) with mRL=1 , in which one of the residues R1RLand R2RL= (CH2)o-CH3 where o = X-4 and the remaining residue R1or R2= (CH2)O-CH3where o = Y-4.

[0067] "monoRL-CXCY" are understood to mean mono-rhamnolipids of the general formula (I) with mRL=1 , in which one of the residues R1RLand R2RL= (CH2)o-CH3 where o = X-4 and the remaining residue R1RLor R2RL= (CH2)o-CH3 where o = Y-4.

[0068] The nomenclature used therefore does not distinguish between "CXCY" and "CYCX".

[0069] For rhamnolipids where mRL=0, monoRL-CX or diRL-CX is used accordingly.

[0070] If one of the above mentioned indices X and / or Y is provided with ":Z", this signifies that the respective residue R1RLand / or R2RLis equal to an unbranched, unsubstituted hydrocarbon residue having X-3 or Y-3 carbon atoms having Z double bonds.

[0071] Methods for preparing the relevant rhamnolipids are disclosed, for example, in EP2786743 and EP2787065; if mixtures of di- and mono-rhamnolipids are obtained, they can be converted with rhamnosidase to the mono-rhamnolipids.

[0072] Rhamnolipids applicable in the context of the instant invention can also be produced by fermentation of Pseudomonas, especially Pseudomonas aeruginosa, which are preferably non genetically modified cells, a technology already disclosed in the eighties, as documented e.g. in EP0282942 and DE4127908. Rhamnolipids produced in Pseudomonas aeruginosa cells which have been improved for higher rhamnolipid titres by genetical modification can also be used in the context of the instant invention; such cells have for example been disclosed by Lei et al. in Biotechnol. Lett. 2020 Jun;42(6):997-1002.

[0073] Rhamnolipids produced by Pseudomonas aeruginosa are commercially available from Jeneil Biotech Inc., e.g. under the tradename Zonix, from Logos Technologies (technology acquired by Stepan), e.g. under the tradename NatSurFact, from Biotensidion GmbH, e.g. under the tradename 201400149 Foreign Filing 7

[0074] Rhapynal, from AGAE technologies, e.g. under the name R90, R95, R95Md, R95Dd, from Locus Bio-Energy Solutions and from Shanghai Yusheng Industry Co. Ltd., e.g. under the tradename Bio- 201 Glycolipids.

[0075] Most of the products mentioned above are mixtures of di- and mono-rhamnolipids which need to be converted to the mono-rhamnolipids, for example with rhamnosidase.

[0076] The compositions according to the instant invention comprises at least one mono-rhamnolipid in solution. The term “mono-rhamnolipid in solution” in the context of the instant convention means, that the mono-rhamnolipids are comprised in the specified amount in the composition according to the instant invention in a liquid phase, so that the mono-rhamnolipids do not settle when centrifuging at 500g for 10 minutes.

[0077] Of course, however, additional rhamnolipids exceeding the specified concentration ranges might be comprised in settable form.

[0078] The total content of said mono-rhamnolipid in solution in a preferred composition according to the instant invention is from 65.0 wt.-% to 76.0 wt.-%, more preferably from 68.0 wt.-% to 74.0 wt.-%, wherein the weight percentages refer to the total composition.

[0079] The content of said organic acid in a preferred composition according to the instant invention is from 1 .0 wt.-% to 7.0 wt.-%, more preferably from 2.0 wt.-% to 5.0 wt.-%, wherein the weight percentages refer to the total composition

[0080] A preferred composition according to the instant invention has total content of said mono- rhamnolipid in solution is from 68.0 wt.-% to 74.0 wt.-% and a content of said organic acid from 2.0 wt.-% to 5.0 wt.-%, wherein the weight percentages refer to the total composition.

[0081] An even more preferred composition according to the instant invention has total content of said mono-rhamnolipid in solution is from 68.0 wt.-% to 74.0 wt.-% and a content of said organic acid from 2.0 wt.-% to 5.0 wt.-%, wherein the weight percentages refer to the total composition, and has a pH at 25°C in the range of 3.3 to 4.5.

[0082] Preferably said mono-rhamnolipid in solution comprised in the composition according to the instant invention is in aqueous solution.

[0083] A preferred composition according to the instant invention is characterised in that the mono- rhamnolipid content in the composition is from 85.0 wt.-% to 100.0 wt.-%, preferably from 95 wt.-% 201400149 Foreign Filing 8 to 100.0 wt.-%, more preferably from 98.0 wt.-% to 100.0 wt.-%, wherein the weight percentages refer to all rhamnolipids comprised in the total composition.

[0084] A preferred composition according to the instant invention is characterised in that the monorhamnolipids comprise

[0085] 12 wt.-% to 32 wt.-% monoRL-C8C10,

[0086] 51 wt.-% to 81 wt.-% monoRL-C10C10,

[0087] 1 wt.-% to 9 wt.-% monoRL-C10C12,

[0088] 1 wt.-% to 9 wt.-% monoRL-C10C12:1 , wherein the weight percentages refer to all mono-rhamnolipids comprised in the composition.

[0089] A preferred composition according to the instant invention is characterised in that the weight ratio between the total content of said mono-rhamnolipid in solution and said organic acid is in the range of from 8.5 : 0.01 to 6.0 : 1 .0, preferably from 8.0 : 0.1 to 6.5 : 0.5, more preferably from 7.5 : 0.2 to 6.8 : 0.4.

[0090] A preferred composition according to the instant invention is characterised in that it has a viscosity of 0.3 Pa s to 5.0 Pa s, preferably 0.5 Pa s to 3.0 Pa s, particularly preferably 0.6 Pa s to 2.0 Pa s, measured in a rheometer at a shear rate of 10 s-1

[0091] The viscosity is measured using a rheometer (MCR 302, Anton Paar Germany) in a parallel plate measuring system. The upper plate has a diameter of 40 mm, the gap distance is 0.5 mm, measuring temperature is 25° C. The measurement was conducted in the shear rate range of 0.1- 100 s-1.

[0092] The compositions according to the instant invention preferably comprise at least one cosmetic active ingredient, preferably selected from ceramides and sphingoid bases.

[0093] In the context of the present invention, the term “ceramide” is understood to mean acylated sphingoid bases, where the sphingoid bases are preferably selected from sphingosine, sphinganine, 6-hydroxysphingosine and phytosphingosine, also in glycosylated form, for example as glucosylceramides.

[0094] The sphingoid base is preferably selected from the group sphingosine, sphinganine, 6- hydroxysphingosine and phytosphingosine.

[0095] A preferred composition according to the instant invention is characterised in that said cosmetic active ingredient is selected from the group comprising, preferably consisting of, ceramide NP, 201400149 Foreign Filing 9 ceramide AP, ceramide EOP, ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NH, ceramide AH and ceramide EOH, preferably selected from the group comprising, preferably consisting of, ceramide NP, ceramide NDS, ceramide AP, ceramide NS, ceramide EOP and ceramide EOS.

[0096] A preferred composition according to the instant invention is characterised in that said cosmetic active ingredient, especially at least on ceramide, is comprised in an amount of from 0.1 wt.-% to 10.0 wt.-%, preferably from 0.5 wt.-% to 7.0 wt.-%, more preferably from -1 .0 wt.-% to 4.0 wt.-%, wherein the weight percentages refer to the total composition.

[0097] The compositions according to the instant invention preferably comprise at least one preservative.

[0098] The compositions according to the instant invention preferably is characterized in that said preservative is selected from the group comprising, preferably consisting of, 4- hydroxyacetophenone, methylparaben, ethylparaben, phenylpropanol, benzyl alcohol, phenethyl alcohol, phenoxyethanol, propanediol, pentylene glycol, 1 ,2-hexanediol, caprylyl glycol, undecyl alcohol, methylpropanediol, ethylhexylglycerin, n-octylglycerin, n-heptylglycerin, n-hexylglycerin, methylheptylglycerin, undecylenamidopropyltrimonium methosulfate, isothiazolinones, preferably selected from chloromethylisothiazolinone (CIT), methylisothiazolinone (MIT), benzisothiazolinone (BIT) , butylbenzisothiazolinone (BBIT), triethyl citrate, citral, hydroxymethylglycinate, glyceryl caprylate, iodopropynyl butylcarbamat and Sodium Caproyl / Lauroyl Lactylate.

[0099] The present invention furthermore provides a process of producing a composition comprising at least one mono-rhamnolipid in solution comprising the steps of

[0100] A) providing a source-composition comprising at least one mono-rhamnolipid, preferably with a content of said mono-rhamnolipid from 0.5 wt.-% to 15.0 wt.-%, preferably from 5 wt.-% to 14.0 wt.-%, more preferably from 8.0 wt.-% to 12.0 wt.-%, wherein the weight percentages refer to the total source-composition,

[0101] B) lowering the pH of said source-composition into the range of 1 .8 to 3.5, preferably 2.2 to 3.4, more preferably 2.5 to 3.2, most preferably 2.6 to 3.1 , by the addition of at least one organic acid and / or its salt selected from the group of benzoic acid, caprylhydroxamic acid, cinnamic acid citric acid, dehydroacetic acid, geranic acid, lactic acid, levulinic acid, mandelic acid, p-anisic acid, pelargonic acid, salicylic acid, sorbic acid, succinic acid, preferably lactic acid, most preferably L- (+)-lactic acid, while obtaining a multi-phase system,

[0102] C) removing the water rich phase from said multi-phase system, and

[0103] D) increasing the pH of the remaining fraction, preferably into a range of 2.5 to 5.5, preferably 2.7 to 5.2, more preferably 3.0 to 4.9, most preferably 3.3 to 4.5. 201400149 Foreign Filing 10

[0104] Preferably the compositions according to the instant invention are produced by the method according to the instant invention.

[0105] Mono-rhamnolipids can be produced by fermentation processes of micro-organisms known in the art. A preferred process according to the instant invention is characterised in that the sourcecomposition provided in step A) is a cell free fermentation broth.

[0106] The term “cell free” in the context of the instant invention means, that the cells of the monorhamnolipid producing organism have been removed, e.g. by centrifugation.

[0107] A preferred process according to the instant invention is characterised in that if in process step B) lactic acid, preferably L-(+)-lactic acid, is added, at least part of said acid is added in its salt form, preferably as sodium lactate.

[0108] A preferred process according to the instant invention is characterised in that in process step C) the pH is increased by means of an inorganic base, in particular alkaline earth metal base or alkali base, preferably aqueous, or ammonia, with NaOH and KOH being particularly preferred.

[0109] The inorganic base preferably is in the form of a solution in water at a concentration of 0.01 M to 15 M, preferably 0.1 M to 5 M.

[0110] The present invention furthermore provides a composition comprising at least one monorhamnolipid in solution obtainable by a process according to the instant invention. This composition according to the instant invention is preferably obtained by a process of the instant invention which consists of the process steps A) to D).

[0111] The present invention furthermore provides a process for the production of a cosmetic or pharmaceutical formulation comprising mono-rhamnolipid and ceramide comprising the steps of

[0112] I) providing a composition according to the instant invention, wherein said composition comprises at least one ceramide and preferably at least one sphingoid base,

[0113] II) formulating said composition with at least one further additional component selected from the group of emollients, emulsifiers, co-emulsifiers, thickeners, viscosity regulators, stabilizers, hydrotropes, solids, fillers, pearlescent additives, opacifiers, insect repellents, self-tanning agents, preservatives, conditioning agents, perfumes, colorants, cosmetic active substances, care additives, refatting agents, electrolytes, UV filters and solvents. 201400149 Foreign Filing 11

[0114] A preferred process according to the instant invention is characterised in that said ceramide is selected from the group comprising, preferably consisting of, ceramide NP, ceramide AP, ceramide EOP, ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NH, ceramide AH and ceramide EOH, preferably selected from the group comprising, preferably consisting of, ceramide NP, ceramide NDS, ceramide AP, ceramide NS, ceramide EOP and ceramide EOS.

[0115] A preferred process according to the instant invention is characterised in that said produced cosmetic or pharmaceutical formulation comprising said at least one ceramide is comprised in an amount of from 0.001 wt.-% to 4.0 wt.-%, preferably from 0.01 wt.-% to 3.5 wt.-%, more preferably from 0.1 wt.-% to 3.0 wt.-%, wherein the weight percentages refer to the total formulation produced.

[0116] The sphingoid base preferably co-comprised in said provided composition is selected from the group sphingosine, sphinganine, 6-hydroxysphingosine and phytosphingosine.

[0117] Substances that can be used as exemplary representatives of the individual groups for said further additional component in process step II) of the process of the instant invention are known to those skilled in the art and can for example be taken from German application DE102008001788.4. This patent application is hereby incorporated as reference and is thus considered to form part of the disclosure.

[0118] As regards said further additional components and also the amounts used of said further additional components, reference is expressly made to the relevant handbooks known to those skilled in the art, for example K. Schrader, “Grundlagen und Rezepturen der Kosmetika” [Fundamentals and formulations of cosmetics], 2nd edition, pages 329 to 341 , Huthig Buch Verlag, Heidelberg.

[0119] The amounts of the respective additives depend on the intended use.

[0120] Typical starting formulations for the relevant applications are known prior art and are contained for example in the brochures of the manufacturers of the relevant base materials and active substances. These existing formulations can generally be adopted unchanged. However, any desired modifications necessary for adjustment and optimization can be made in a straightforward manner through simple tests.

[0121] Preferred said further additional component in process step II) comprises water and preferably cholesterol. 201400149 Foreign Filing 12

[0122] In a preferred process according to the instant invention in process step I) a composition according to the instant invention comprising a ceramide selected from ceramide NP and ceramide NDS and phytosphingosine and preferably additionally ceramide AP and ceramide EOP, is provided and said further additional component in process step II) comprises water and preferably cholesterol.

[0123] The present invention furthermore provides the use of a composition according to the instant invention for the production of an end-customer product, preferably a formulation, especially a cleaning or care formulation, more preferably a household care or cosmetic formulation.

[0124] The cleaning formulation preferably is a laundry formulation, a dish washing formulation, a car cleaning formulation, a hard floor cleaning formulation, a glass cleaner, a bathroom cleaner, a kitchen cleaner, an over cleaner, an all purpose cleaner, a wet wipe, a metal cleaner, a membrane cleaner for food and beverage applications, a CIP (cleaning in place) cleaner, a big kitchen cleaner, a traffic film remover, a carpet cleaner, an alkaline cleaner, a prespotter, a mild cleaner or a neutral cleaner.

[0125] The examples adduced hereinafter describe the present invention by way of example, without any intention that the invention, the scope of application of which is apparent from the entirety of the description and the claims, be restricted to the embodiments specified in the examples.

[0126] Mono-rhamnolipids were prepared as in example 2 of EP3061442.

[0127] Example 1: Viscosity of mono rhamnolipids and lactic acid.

[0128] To a highly concentrated monoRL L-(+)-lactic acid was added. Four different samples were prepared, three containing 2%, 3% and 5% of L-(+)-lactic acidrespectively and one without any L- (+)-lactic acid. The viscosities of all four samples were determined.

[0129] Table 1 : All samples were prepared to a mass of 30g by adjusting the pH to 4 with KOH and adding additional water. 201400149 Foreign Filing 13

[0130] Table 1 : Examples 1a - 1d.

[0131] The viscosity is measured using a rheometer (MCR 302, Anton Paar Germany) in a parallel plate measuring system. The upper plate has a diameter of 40 mm, the gap distance is 0.5 mm, measuring temperature is 25° C. The measurement was conducted in the shear rate range of 0.1- 100 s-1.

[0132] At a pH value of 4, for the combination including 2% L-(+)-lactic acid a viscosity of 1 .06 Pa s was measured, for the composition with 3% L-(+)-lactic acid a viscosity of 1 .01 Pa s was measured. Upon addition of 5% L-(+)-lactic acid a viscosity of 0.99 Pa s at a share rate of 10 1 / s was determined. Comparing these results to the viscosities without L-(+)-lactic acid significant differences can be observed. Without L-(+)-lactic acid and at a pH value of 4 a viscosity of 2.25 Pa s at a share rate of .10 1 / s was measured. This is a significantly higher compared to the combination with L-(+)-lactic acid.

[0133] Table 2: Viscosity (Pa s, shear rate 10 1 / s) of mono-rhamnolipid and L-(+)-lactic acid combination at a certain pH value. 201400149 Foreign Filing 14

[0134] Example Formulations: In each of the following example-formulations the three compositions of the instant invention example 1a, example 1 b and example 1c were used respectively (“Composition A”).

[0135] Table 3: Cleansing Gel

[0136] Table 4: Clear Conditioning Hair and Body Shampoo Table 5: Cleansing Foam 201400149 Foreign Filing 15

[0137] Table 6: Body Wash Table 7: Micellar Water 201400149 Foreign Filing 16

[0138] Table 8: Micellar Water 2.

[0139] Table 9: Liquid Serum or Toner.

[0140] Table 10: Repairing Conditioner. 201400149 Foreign Filing 17

[0141] Table 11 : Hair Conditioner. Table 12: Milky Creamy Cleanser.

[0142] Table 13: Creamy Cleanser. 201400149 Foreign Filing 18

[0143] Table 14: Body Cleanser.

[0144] Table 15: Shampoo Formulation.

[0145] Table 16: Conditioning Shampoo. 201400149 Foreign Filing 19

[0146] Table 18: Conditioning Ceramide Shampoo

[0147] Table 19: Ceramide Cleansing Gel 201400149 Foreign Filing 20

[0148] Table 20: Sensitive Shampoo Table 21 : Anti Dandruff Shampoo 201400149 Foreign Filing 21

[0149] Table 22: Classic Shampoo 201400149 Foreign Filing 22

[0150] Table 23: Shampoo for Sensitive Scalp 201400149 Foreign Filing 23

[0151] Table 24: Shampoo for Curls

[0152] Table 25: Repair Shampoo 201400149 Foreign Filing 24 201400149 Foreign Filing 25

[0153] Table 26: Repair Shampoo 201400149 Foreign Filing 26

[0154] Table 27: Shampoo for Glossy Hair 201400149 Foreign Filing 27

[0155] Table 28: Bond Repair Shampoo 201400149 Foreign Filing 28

[0156] Table 29: Repair Shampoo 201400149 Foreign Filing 29 201400149 Foreign Filing 30

[0157] Table 30: Shampoo for Glossy Hair 201400149 Foreign Filing 31

[0158] Table 31 : Volume Shampoo

[0159] Table 32: Soothing Shampoo 201400149 Foreign Filing 32

[0160] Table 33: Classic Anti Dandruff Shampoo 201400149 Foreign Filing 33

[0161] Table 34: Hydrating Shampoo 201400149 Foreign Filing 34

[0162] Table 35: Hydrating Shampoo with Actives 201400149 Foreign Filing 35

[0163] Table 36: Sensitive Conditioner

[0164] Table 37: Intense Repair Conditioner 201400149 Foreign Filing 36

[0165] Table 38: Strengthening Conditioner 201400149 Foreign Filing 37

[0166] Table 39: Spray Conditioner 201400149 Foreign Filing 38

[0167] Table 40: Hydrating Conditioner

[0168] Table 41 : Conditioner for Coloured Hair 201400149 Foreign Filing 39

[0169] Table 42: Conditioner for Hydration and Shine 201400149 Foreign Filing 40

[0170] Table 43: Oat Conditioner 201400149 Foreign Filing 41

[0171] Table 44: Deep Repairing Conditioner 201400149 Foreign Filing 42

[0172] Table 45: Deep Repairing Conditioner 201400149 Foreign Filing 43

[0173] Table 46: Rich Repair Conditioner 201400149 Foreign Filing 44

[0174] Table 47: Light Conditioner

[0175] Table 48: Soft Conditioner 201400149 Foreign Filing 45

[0176] Table 49: Colour Conditioner 201400149 Foreign Filing 46

[0177] Table 50: Leave-In Conditioner

[0178] Table 51 : Conditioner with Actives 201400149 Foreign Filing 47

[0179] Table 52: Refreshing Micellar Water 201400149 Foreign Filing 48

[0180] Table 53: Micellar Water

[0181] Table 54: Soothing Micellar Water 201400149 Foreign Filing 49

[0182] Table 55: Bi-Phasic Aloe Vera Micellar Water 201400149 Foreign Filing 50

[0183] Table 56: Rose Micellar Water

[0184] Table 57: Deep Cleansing Micellar Water 201400149 Foreign Filing 51

[0185] Table 58: Ficus Micellar Water

[0186] Table 59: Witch Hazel Micellar Water 201400149 Foreign Filing 52

[0187] Table 60: 3 in 1 Micellar Water

[0188] Table 61 : Cleaning Micellar Water 201400149 Foreign Filing 53

[0189] Table 62: Micellar Water for Sensitive Skin 201400149 Foreign Filing 54

[0190] Table 63: Softening Micellar Water

[0191] Table 64: Deep Cleansing Micellar Water Table 65: Micellar Water I 201400149 Foreign Filing 55

[0192] Table 66: Pure 3-in-1 Micellar Water 201400149 Foreign Filing 56

[0193] Table 67: Micellar Water with Actives 201400149 Foreign Filing 57

[0194] Table 68: Very Sensitive Shower Gel

[0195] Table 69: Happy Shower Gel 201400149 Foreign Filing 58

[0196] Table 70: Lemongrass Shower Oil 201400149 Foreign Filing 59

[0197] Table 71 : Shower Oil

[0198] Table 72: Berry Shower Foam Table 73: Refreshing Shower Foam 201400149 Foreign Filing 60

[0199] Table 74: Brilliance Shower Oil 201400149 Foreign Filing 61

[0200] Table 75: Shower Gel II

[0201] Table 76: Energy Shower Gel 201400149 Foreign Filing 62

[0202] Table 77: Natural Shower Gel

[0203] Table 78: Luxurious Shower Oil 201400149 Foreign Filing 63

[0204] Table 79: Rosemary Shower Oil 201400149 Foreign Filing 64

[0205] Table 80: Sesame Shower Oil 201400149 Foreign Filing 65

[0206] Table 81 : Happy Shower Oil

[0207] Table 82: Vitality Bubble Bath 201400149 Foreign Filing 66

[0208] Table 83: Rich Shower Cream 201400149 Foreign Filing 67

[0209] Table 84: Wash Cream for Babies Table 85: Natural Shower Cream 201400149 Foreign Filing 68

[0210] Table 86: Dreamy Shower Cream 201400149 Foreign Filing 69

[0211] Table 87: Shower Gels with Actives 201400149 Foreign Filing 70

[0212] Table 88: Hydro Cleansing Gel

[0213] Table 89: Grape Cleansing Oil 201400149 Foreign Filing 71

[0214] Table 90: Ultra Sensitive Cleansing Gel 201400149 Foreign Filing 72

[0215] Table 91 : Vitamin C Cleansing Gel

[0216] Table 92: Cleansing Milk 201400149 Foreign Filing 73

[0217] Table 93: Wild Roses Cleansing Milk 201400149 Foreign Filing 74

[0218] Table 94: Vitamin C Cleansing Foam

[0219] Table 95: Cleansing Foam for Kids 201400149 Foreign Filing 75

[0220] Table 96: Soft Cleansing Gel 201400149 Foreign Filing 76

[0221] Table 97: Honey Cleansing Gel

[0222] Table 98: Clean Cleansing Gel 201400149 Foreign Filing 77

[0223] Table 99: Soft Cleansing Milk 201400149 Foreign Filing 78

[0224] Table 100: Natural Cleansing Milk

[0225] Table 101 : Cleansing Foam for Kids 201400149 Foreign Filing 79

[0226] Table 102: Thermal Cleansing Foam 201400149 Foreign Filing 80

[0227] Table 103: Cleansing Gel with Actives

[0228] Table 104: Bi Phasic Make-Up Remover 201400149 Foreign Filing 81

[0229] Table 105: Waterproof Make-Up Entferner

[0230] Table 106: Effective Make Up Remover 201400149 Foreign Filing 82

[0231] Table 107: Calendula Make Up Remover

[0232] Table 108: BiPhasic Make Up Remover 201400149 Foreign Filing 83

[0233] Table 109: Eye Make-Up Remover 201400149 Foreign Filing 84

[0234] Table 110: Micellar Make-Up Remover

[0235] Table 111 : Oil free Make Up Remover 201400149 Foreign Filing 85

[0236] Table 112: 2in1 Make Up Remover

[0237] Table 113: 2 Phase Make Up Remover 201400149 Foreign Filing 86

[0238] Table 114: Fig Cleansing Gel and Make Up Remover

[0239] Table 115: Bi Phasic Make Up Remover 201400149 Foreign Filing 87

[0240] Table 116: Eye Make-Up Remover

[0241] Table 117: Eye Make Up Remover 201400149 Foreign Filing 88

[0242] Table 118: Very Efficient Make Up Remover Table 119: Make Up Remover with Actives 201400149 Foreign Filing 89

[0243] Table 120: Simple Cleansing Oil 201400149 Foreign Filing 90

[0244] Table 121 : Soft Cleansing Oil

[0245] Table 122: Soothing Cleansing Oil for Sensitive Skin Table 123: Camomile Cleansing Oil 201400149 Foreign Filing 91

[0246] Table 124: Deep Cleansing Cleansing Oil

[0247] Table 125: Olive Oil Cleansing Oil Table 126: Hydroxytyrosol Cleansing Oil 201400149 Foreign Filing 92

[0248] Table 127: Derma Cleansing Oil

[0249] Table 128: Vitamin E Cleansing Oil 201400149 Foreign Filing 93

[0250] Table 129: Mushroom Cleansing Oil

[0251] Table 130: Micellar Cleansing Oil 201400149 Foreign Filing 94

[0252] Table 131 : Fresh Cleansing Oil

[0253] Table 132: Resetting Cleansing Oil 201400149 Foreign Filing 95

[0254] Table 133: Cleansing Oil to Milk Table 134: Superfood Cleansing Face Oil 201400149 Foreign Filing 96

[0255] Table 135: Cleansing Oil with Actives 201400149 Foreign Filing 97

[0256] Table 136: Essential Toner

[0257] Table 137: Ultra Sensitive Facial Toner 201400149 Foreign Filing 98

[0258] Table 138: Wildrose Facial Toner

[0259] Table 139: Age Perfect Facial Toner Table 140: Peeling Toner 201400149 Foreign Filing 99

[0260] Table 141 : Beauty Toner 201400149 Foreign Filing 100

[0261] Table 142: BHA Toner 201400149 Foreign Filing 101

[0262] Table 143: Moisture Boost Toner

[0263] Table 144: Clearing Facial Toner Table 145: Revitalizing Facial Toner 201400149 Foreign Filing 102

[0264] Table 146: Hydrating Toner

[0265] Table 147: Soft Facial Toner 201400149 Foreign Filing 103

[0266] Table 148: Classic Toner

[0267] Table 149: Calming Toner 201400149 Foreign Filing 104

[0268] Table 150: Balancing Toner 201400149 Foreign Filing 105

[0269] Table 151 : Toner with Actives 201400149 Foreign Filing 106

[0270] Table 152: Soft Cleansing Wipes

[0271] Table 153: 3in1 Cleansing Wipes 201400149 Foreign Filing 107

[0272] Table 154: Soothing Cleansing Wipes 201400149 Foreign Filing 108

[0273] Table 155: Wildrose Cleansing Wipes

[0274] Table 156: Aloe Wet Wipes 201400149 Foreign Filing 109

[0275] Table 157: Wet Wipes for Face & Hands

[0276] Table 158: Water Wet Wipes

[0277] Table 159: Micellar Cleansing Wipes 201400149 Foreign Filing 110

[0278] Table 160: Make Up Remover Wipes 201400149 Foreign Filing 111

[0279] Table 161 : Detox Cleansing Wipes 201400149 Foreign Filing 112

[0280] Table 162: Beard Cleansing Wipes

[0281] Table 163: Cleansing Wipes with Actives 201400149 Foreign Filing 113

[0282] Table 164: Vitamin C Serum 201400149 Foreign Filing 114

[0283] Table 165: Vit C Serum

[0284] Table 166: Glow Serum Table 167: Niacinamide Serum 201400149 Foreign Filing 115

[0285] Table 168: Anti Wrinkles Serum 201400149 Foreign Filing 116

[0286] Table 169: Hyaluronic Acid Serum

[0287] Table 170: Olive Serum 201400149 Foreign Filing 117

[0288] Table 171 : Pore Minimizer Serum 201400149 Foreign Filing 118

[0289] Table 172: Ceramide Serum

[0290] Table 173: Glowing Serum 201400149 Foreign Filing 119 201400149 Foreign Filing 120

[0291] Table 174: Lifting Serum 201400149 Foreign Filing 121

[0292] Table 175: Hydra Serum 201400149 Foreign Filing 122

[0293] Table 176: Almond Serum 201400149 Foreign Filing 123

[0294] Table 177: Brilliance Serum 201400149 Foreign Filing 124

[0295] Table 178: Scalp Serum 201400149 Foreign Filing 125

[0296] Table 179: Serum with Actives 201400149 Foreign Filing 126

[0297] Examples: Formulation for Cleaning Solution Table 180: Formulation for washing wool

[0298] Table 181 : Compositions of Formulations used in environmental stress cracking tests 201400149 Foreign Filing 127

[0299] Table 182: Formulations for hard surface cleaning

[0300] Table 183: Cleaning Formulation for lipstick from skin

[0301] Table 184: Formulation for cleaning and greying inhibition / antiredeposition of Soil 201400149 Foreign Filing 128

[0302] Table 185: Powder detergent

[0303] Table 186: Liquid detergent

[0304] Table 187: Liquid detergent concentrate 201400149 Foreign Filing 129

[0305] Table 188: Detergent Formulation 4 & 5 Table 190: Detergent 6 201400149 Foreign Filing 130

[0306] Table 191 : Detergent 7

[0307] Table 192: Detergent 8

[0308] Table 193: Detergent 9 201400149 Foreign Filing 131

[0309] Table 194: Detergent 10

[0310] Table 195: Detergent 11 201400149 Foreign Filing 132

[0311] Table 196: Detergent 12 Table 197: Detergent 13

[0312] Table 198: Detergent 14 201400149 Foreign Filing 133

[0313] Table 199: Detergent 15

[0314] Table 200: Detergent 16

[0315] Table 201 : Detergent 17 201400149 Foreign Filing 134

[0316] Table 202: Detergent 18

[0317] Table 203: Detergent 19

[0318] Table 204: Detergent 20 201400149 Foreign Filing 135

[0319] Table 205: Detergent 21

[0320] Table 206: Detergent 22 201400149 Foreign Filing 136

[0321] Table 207: Detergent 23

[0322] Table 208: Detergent 24 201400149 Foreign Filing 137

[0323] Table 209: Detergent 25

[0324] Table 210: Detergent 26

[0325] Table 211 : Detergent 27 201400149 Foreign Filing 138

[0326] Table 212: Detergent 28

[0327] Table 213: Detergent 29

[0328] Table 214:Detergent 30 201400149 Foreign Filing 139

[0329] Table 215: Detergent 31 201400149 Foreign Filing 140

[0330] Table 216: Detergent 32 Table 217: Detergent 33

[0331] Table 218: Detergent 34 201400149 Foreign Filing 141

[0332] Table 219: Detergent 35

[0333] Table 220: Detergent 36 201400149 Foreign Filing 142

[0334] Table 221 : Detergent 37

[0335] Table 222: Detergent 38

[0336] Table 223: Detergent 39 201400149 Foreign Filing 143

[0337] Table 224: Bio pre-spotter

[0338] Table 225: Bio pre-spotter 2

[0339] Table 226: Pre-spotter 3 201400149 Foreign Filing 144

[0340] Table 227: Green Hand Dish Wash Formulation 1

[0341] Table 228: Green Hand Dish Wash Formulation 2

[0342] Table 229: Green Hand Dish Wash Formulation 3 201400149 Foreign Filing 145

[0343] Table 230: Car Shampoo. High Degreasing

[0344] Table 231 : Car Shampoo

[0345] Table 232: Car Shampoo. Triple Foam Table 233: Traffic Film Remover

[0346] Table 234: Traffic Film Remover for Road Signs 201400149 Foreign Filing 146

[0347] Table 235: Windshield Cleanser. Winter

[0348] Table 236: Truck and Canvas Cleanser. Dilutable

[0349] Table 237: Carpet Cleaner with Odor Removal

[0350] Table 238: Metal Cleaner

[0351] Table 239: Alkaline Metal Cleaner 1 201400149 Foreign Filing 147

[0352] Table 240: Alkaline Metal Cleaner 2

[0353] Table 241 : Mild Metal Cleaner

[0354] Table 242: Bathroom Cleanser

[0355] Table 243: Mild All-Purpose Cleaner 1 201400149 Foreign Filing 148

[0356] Table 244: Mild All-Purpose Cleaner 2

[0357] Table 245: Mild All-Purpose Cleaner 3

[0358] Table 246: Mild All-Purpose Cleaner with Cleaning Booster

[0359] Table 247: F&B All purpose Spray 201400149 Foreign Filing 149

[0360] Table 249: All-Purpose Cleaner. Ready to Use

[0361] Table 250: Alkaline Cleanser

[0362] Table 251 : Hard Surface Degreaser

[0363] Table 252: Degreaser spray 1

[0364] Water (Dye. Perfume) 95.00 201400149 Foreign Filing 150

[0365] Table 253: Degreaser spray 2

[0366] Table 255: F&B Membrane Cleaning 1

[0367] Table 256: F&B Membrane Cleaning 2 201400149 Foreign Filing 151

[0368] Table 257: Kitchen Cleaning Spray

[0369] Table 258: High Performance Kitchen Cleaner

[0370] Table 259: Ready to Use Kitchen Cleaner

[0371] Table 260: Floor Cleaner. Superspreading 201400149 Foreign Filing 152

[0372] Table 261 : Floor Cleanser. Highly Efficient

[0373] Table 262: Plastic Cleanser

[0374] Table 263: Glass Cleaner 1 Table 264: Glass Cleaner 2

[0375] Table 265: Glass Cleaner 3 201400149 Foreign Filing 153

[0376] Table 266: Formulation of different detergents Table 267: Detergent Formulation

[0377] Table 268: Detergent Formulations 201400149 Foreign Filing 154

[0378] Table 269: Detergent Formulations

[0379] Table 270: Detergent Formulations 201400149 Foreign Filing 155

[0380] Table 271 : Detergent Formulations

[0381] Table 272: Detergent Formulations

[0382] Table 273: Detergent Formulations 201400149 Foreign Filing 156

[0383] Table 274: Liquid detergent

[0384] Table 275: Liquid detergent 201400149 Foreign Filing 157

[0385] Table 276: Liquid detergent Table 277: Liquid detergent 201400149 Foreign Filing 158

[0386] Table 278: Liquid detergent

[0387] Table 279: Liquid detergent 201400149 Foreign Filing 159

[0388] Table 280: Liquid detergent

[0389] Table 281 : Liquid detergent 201400149 Foreign Filing 160

[0390] Table 282: Liquid detergent

[0391] Table 283: Liquid detergent

[0392] Table 284: Liquid detergent 201400149 Foreign Filing 161

[0393] Table 285: Liquid detergents

[0394] Table46

[0395] Table 286: Liquid detergent 201400149 Foreign Filing 162

[0396] Table 287: Liquid detergent

[0397] Table 288: Liquid detergent

[0398] Table 289: Liquid detergent 201400149 Foreign Filing 163

[0399] Table 290: Liquid detergent

[0400] Table 291 : Liquid detergent 201400149 Foreign Filing 164

[0401] Table 292: Liquid detergent

[0402] Table 293: Liquid detergent

[0403] Table 294: Laundry pre-spotter 201400149 Foreign Filing

Claims

201400149 Foreign Filing 166Claims1 . A composition comprising at least one mono-rhamnolipid in solution and at least one organic acid and / or its salt selected from the group of benzoic acid, caprylhydroxamic acid, cinnamic acid citric acid, dehydroacetic acid, geranic acid, lactic acid, levulinic acid, mandelic acid, p-anisic acid, pelargonic acid, salicylic acid, sorbic acid, succinic acid, preferably lactic acid, wherein the total content of said mono-rhamnolipid in solution is from 60.0 wt.-% to 80.0 wt.-%, preferably from 65.0 wt.-% to 76.0 wt.-%, more preferably from 68.0 wt.-% to 74.0 wt.-%, and wherein the content of said organic acid is from 0.1 % wt.-% to 10.0 wt.-%, preferably from 1 .0 wt.-% to 7.0 wt.-%, more preferably from2.0 wt.-% to 5.0 wt.-%, wherein the weight percentages refer to the total composition, characterized in that the pH of the composition at 25 °C is in the range of 2.5 to 5.5, preferably 2.7 to 5.2, more preferably 3.0 to 4.9, most preferably 3.3 to 4.5.

2. A composition according to claim 1 characterised in that the mono-rhamnolipid content in the composition is from 85.0 wt.-% to 100.0 wt.-%, preferably from 95 wt.-% to 100.0 wt.-%, more preferably from 98.0 wt.-% to 100.0 wt.-%, wherein the weight percentages refer to all rhamnolipids comprised in the total composition.

3. A composition according to claim 1 or 2 characterised in that the mono-rhamnolipids comprise12 wt.-% to 32 wt.-% monoRL-C8C10,51 wt.-% to 81 wt.-% monoRL-C10C10,1 wt.-% to 9 wt.-% monoRL-C10C12,1 wt.-% to 9 wt.-% monoRL-C10C12:1 , wherein the weight percentages refer to all mono-rhamnolipids comprised in the composition.

4. A composition according to at least one of the preceding claims characterised in that the weight ratio between the total content of said mono-rhamnolipid in solution and said organic acid is in the range of from 8.5 : 0.01 to 6.0 : 1.0, preferably from 8.0 : 0.1 to 6.5 : 0.5, more preferably from 7.5 : 0.2 to 6.8 : 0.4.

5. A composition according to at least one of the preceding claims characterised in that it has a viscosity of 0.3 Pa s to 5 Pa s, preferably 0.5 Pa s to 3 Pa s, particularly preferably 0.6 Pa s to 2 Pa s, measured in a rheometer at a shear rate of 10 s-1.201400149 Foreign Filing 1676. A composition according to at least one of the preceding claims characterised in that it comprises at least one cosmetic active ingredient, preferably selected from ceramides and sphingoid bases.

7. A composition according to claim 6 characterised in that said cosmetic active ingredient is selected from the group comprising ceramide NP, ceramide AP, ceramide EOP, ceramide NDS, ceramide ADS, ceramide EODS, ceramide NS, ceramide AS, ceramide EOS, ceramide NH, ceramide AH and ceramide EOH, preferably selected from the group comprising, preferably consisting of, ceramide NP, ceramide NDS, ceramide AP, ceramide NS, ceramide EOP and ceramide EOS.

8. A composition according to claim 6 or 7 characterised in that said cosmetic active ingredient is comprised in an amount of from is from 0.1 wt.-% to 10.0 wt.-%, wherein the weight percentages refer to the total composition.

9. A composition according to at least one of the preceding claims characterised in that it comprises at least one preservative.

10. A composition according to claim 9 characterised in that the preservative is selected from the group comprising4-hydroxyacetophenone, methylparaben, ethylparaben, phenylpropanol, benzyl alcohol, phenethyl alcohol, phenoxyethanol, propanediol, pentylene glycol, 1 ,2-hexanediol, caprylyl glycol, undecyl alcohol, methylpropanediol, ethylhexylglycerin, n-octylglycerin, n-heptylglycerin, n-hexylglycerin, methylheptylglycerin, undecylenamidopropyltrimonium methosulfate, isothiazolinones, triethyl citrate, citral, hydroxymethylglycinate, glyceryl caprylate, iodopropynyl butylcarbamat and Sodium Caproyl / Lauroyl Lactylate.

11. A process of producing a composition comprising at least one mono-rhamnolipid in solution comprising the steps of201400149 Foreign Filing 168A) providing a source-composition comprising at least one mono-rhamnolipid, preferably with a content of said mono-rhamnolipid from 0.5 wt.-% to 15.0 wt.-%, preferably from 5 wt.-% to 14.0 wt.-%, more preferably from 8.0 wt.-% to 12.0 wt.-%, wherein the weight percentages refer to the total source-composition,B) lowering the pH of said source-composition into the range of 1 .8 to 3.5, preferably 2.2 to 3.4, more preferably 2.5 to 3.2, most preferably 2.6 to 3.1 , by the addition of at least one organic acid and / or its salt selected from the group of benzoic acid, caprylhydroxamic acid, cinnamic acid citric acid, dehydroacetic acid, geranic acid, lactic acid, levulinic acid, mandelic acid, p-anisic acid, pelargonic acid, salicylic acid, sorbic acid, succinic acid, preferably lactic acid, while obtaining a multi-phase system,C) removing the water rich phase from said multi-phase system, andD) increasing the pH of the remaining fraction, preferably into a range of 2.5 to 5.5, preferably 3.0 to 5.0, more preferably 3.6 to 4.5, most preferably 3.8 to 4.3.

12. A process according to claim 11 characterised in that the source-composition provided in step A) is a cell free fermentation broth.

13. A composition comprising at least one mono-rhamnolipid in solution obtainable by a process according to at least one of the claims 11 or 12.

14. A process for the production of a cosmetic or pharmaceutical formulation comprising mono- rhamnolipid and ceramide comprising the steps ofI) providing a composition according to any of the claims 1 to 10 and 13, wherein said composition comprises at least one ceramide and preferably at least one sphingoid base,II) formulating said composition with at least one further additional component selected from the group of emollients, emulsifiers, co-emulsifiers, thickeners, viscosity regulators, stabilizers, hydrotropes, solids, fillers, pearlescent additives, opacifiers, insect repellents, selftanning agents, preservatives, conditioning agents, perfumes, colorants, cosmetic active substances, care additives, refatting agents, electrolytes, UV filters and solvents.

15. Use of a composition according to at least one of the claims 1 to 10 or 13 for the production of an end-customer product, preferably a formulation, especially a cleaning or care formulation, more preferably a household care or cosmetic formulation.

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