Disinfectant composition against single-celled eukaryotic organisms

Abstract

The present invention relates to a disinfectant composition against parasitic organisms. The invention also relates to a method for disinfecting at least one area to prevent parasitosis. The invention also relates to the use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

Description

[0001] DIS INFECTANT COMPOS ITION AGAINST S INGLE-CELLED EUKARYOTIC

[0002] ORGANISMS

[0003] The present invention relates to a disinfectant composition against parasitic organisms . The invention also relates to a method for disinfecting areas to prevent parasitosis , in particular areas for keeping, housing and / or transporting animals . The invention also relates to the use of one or more compounds selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms , in particular eggs or oocysts , of parasitic organisms .

[0004] In the context of the invention the disinfectant composition accordingly relates in particular to a disinfection composition for prevention of parasitosis of livestock . Parasitosis in this respect refers to infestation with or a disease caused by parasites .

[0005] In intensive livestock farming, infection by parasites represents a substantial risk . Despite the availability of various medical treatments of infections resulting from parasitosis , the use of medical treatment has no lasting ef fect .

[0006] This is because animal feces may contain highly resistant durable forms ( in particular eggs and oocysts ) of parasites , which are invasive and can re-enter the animal ' s body by ingestion .

[0007] Therefore , normal hygiene procedures in the area where the animals are kept should be supplemented with additional disinfection procedures to mitigate the risk of parasitosis . Various disinfection agents are used to disinfect the living areas of livestock in order to combat this problem and prevent or at least reduce the occurrence of parasitosis .

[0008] Available disinfectant compositions may comprise biocidal phenolic compounds , including phenol , substituted phenols , cresols and halogenated cresols , for instance as described in US patent 5 , 620 , 655 or Australian patent application AU 2012241180 Al .

[0009] A di f ficulty in prevention of parasitosis by treating living areas of livestock with disinfectants lies in the penetration of the very tough membranes of parasitic durable forms such as oocysts and eggs , and thus to enable the entry into the interior of these parasitic durable forms in order to kill or damage the parasites irreversibly .

[0010] As a result , high concentrations of biocides may often be necessary to achieve the desired reduction or elimination of parasites . Given the fact that high concentrations of these active compounds may also be harmful for the persons involved with this , or may negatively impact the environment , it is desirable to lower the amounts of the active compounds by improving penetration of the parasites .

[0011] The abovementioned Australian patent application AU 2012241180 Al suggests addressing this problem of penetration by the provision of a disinfectant comprising a combination of di f ferent biocidal phenols or phenol derivatives in combination with keratolytics , in particular salicylic acid .

[0012] The compositions described in US patent 5 , 620 , 655 or Australian patent application AU 2012241180 Al still require a relatively high concentration of biocide to kill or damage the parasites irreversibly . US 3,932, 684 discloses phenolic agents for disinfecting stalls and uses 1:12 (8,3%) dilutions of a composition of 10 parts by weight of p-chloro-m-cresol , 10 parts by weight of perchloroethylene, 10 parts by weight of an aqueous 50% solution of the surface-active compound sodium alkylbenzene sulfonate having 8 to 20 carbon atoms in the alkyl and 15 parts by weight of isopropanol (example 1 of US 3,932, 684) and 1:12 (8,3%) dilutions of a composition of 15 parts by weight of o-phenyl-phenol , 10 parts by weight of perchloroethylene, 10 parts by weight of an aqueous 50% solution of the surface-active compound sodium alkylbenzene sulfonate having 8 to 20 carbon atoms in the alkyl, 30 parts by weight of isopropanol, and 35 parts by weight of distilled water (example 2 of US 3,932, 684) .

[0013] The present inventors consider that there is room for improvement and aim to provide a disinfectant composition which shows improved penetration and killing of parasites, thereby allowing the use of reduced concentrations of active biocides .

[0014] Summary of the invention.

[0015] For this purpose, the present invention relates in a first aspect to a disinfectant composition comprising at least one active compound against parasitic organisms; and at least one compound selected from the group of fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms; wherein a weight ratio of said at least one active compound to said at least one compound selected from fatty acids and fatty alcohols is in the weight range of 1:10 to 6:1.

[0016] In a second aspect the invention relates to a method for disinfecting at least one area to prevent parasitosis, wherein the method comprises disinfecting said area with an ef fective amount of the disinfectant composition according to the first aspect .

[0017] In a third aspect the invention relates to the use of one or more compounds selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of active compounds contained in said composition into the durable forms , in particular eggs or oocysts , of parasitic organisms .

[0018] Short description of the drawings

[0019] Fig . 1 shows four groups of Eimeria species , in particular a mixture of E. tenella and E. acervulina, classi fied on their state . A: no change in structure of the oocyst observed, B : the cell-wall is intact , however, the internal structure of the oocyst is lost , C : cell wall is damaged and the cell is leaking, D : cell wall is completely broken and the interior components have leaked out substantially .

[0020] Detailed description of the invention

[0021] The inventors have surprisingly found that the use of one or more compounds selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition improves penetration of active compounds contained in said composition into the durable forms , in particular eggs or oocysts , of parasitic organisms .

[0022] The active compound of the present invention relates to a biocidal agent that is active against parasitic organisms , and in particular their durable forms , such as eggs and oocysts . In the context of the present invention the composition may be suitable for killing or irreversibly damaging any durable form of a parasite , in particular ascarides and coccidia, or other unicellular eukaryotic parasites . The composition of the invention is designed to penetrate the very tough membranes of such parasites and in particular their parasitic durable forms . In principle the present invention thus finds particular use for these purposes . Nevertheless , although parasiticidal action is the main incentive of the present invention, bactericidal , fungicidal or virucidal action may be an inevitable and further advantageous side ef fect .

[0023] The range of application of the present invention in particular lies in the field of livestock farming .

[0024] Ascarides and coccidia are known to cause maj or problems in animal husbandry .

[0025] Accordingly, the parasite in the context of the invention may be any of the group of ascarides or coccidia . A particular relevant ascaride i s Ascari s , in particular Ascari s suum . Ascarides belong to the genus of roundworms . Ascarides eggs have an eggshell comprising three layers . The middle layer of chitin-like substances and the inner vitelline membrane are di f ficult to penetrate .

[0026] Particular relevant coccidia include Eimeria speci es , including without limitation E. tenella , E. acervulina E. maxima, E. brunet ti , E. neca trix , with particular relevance for Eimeria tenella . Eimeria oocysts are also di f ficult to penetrate because parasite speci fic disinfectants first have to penetrate the lipid-containing outer walls of the oocyst and to attack the stable glycoproteins of the inner walls before they can achieve irreversible damage .

[0027] Other relevant parasites include Cryptosporidia parasites . Also Cryptosporidi um oocysts are resistant to many commonly used disinfectants due to their thick and impermeable cell wall and for most chemicals , ef fective concentrations are generally not practical for disinfection because highly concentrated chemicals that would signi ficantly reduce oocyst infectivity are either very expensive or very harmful for the persons involved with it or for the environment in general .

[0028] A further parasite of the order coccidia is Cystoi sospora sui s, which causes porcine cystoisosporosis predominantly in suckling piglets .

[0029] The at least one active compound against parasitic organisms in the context of the invention may be suitably chosen amongst the known biocidal agents for purposes of fighting the abovementioned parasites .

[0030] Active compounds according to the invention may be characteri zed in that they contain at least one phenolic active disinfection ingredients . These may include , without limitation, phenol , substituted phenols , cresols and halogenated cresols , in particular 2 -methylphenol , 3- methylphenol , 4 -methylphenol , 4-ethylphenol , 2 , 4- dimethylphenol , 2 , 5-dimethylphenol , 3 , 4-dimethylphenol , 2 , 6-dimethylphenol , 4-n-propylphenol , 4-n-butylphenol , 4-n- amylphenol , 4-n-hexylphenol , thymol , iso-thymol ( also known as carvacrol or monoterpenoid phenol ) o-cyclohexyl-p- chlorophenol , o-n-amyl-p-chorophenol , o-n-hexyl-p- chlorophenol , p-chloro-m-cresol , 4-tert . -butyl-2 , 6- dichlorophenol , 6-tert . -butyl-4-chloro-m-cresol , 4-ethyl-4- chlorophenol , 4-chloro-3 , 5-xylenol , 2 , 4-dichloro-3 , 5- xylenol , p-phenyl phenol , o-phenyl phenol , 2-benzylphenol , p-chloro-o-phenyl phenol , benzyl-4-chloro-m-cresol , and 4- chlorobenzyl-dichloro-m-cresol .

[0031] Preferred active compounds may be selected from the group of orthophenylphenol ( OPP ) , chloro-cresol ( Cl- cresol ) , chloro-xylenol , 4-t-amylphenol ( 4-TAP ) , amylmetacresol , dichlorobenzyl alcohol ( DCBA) , carvacrol , diclosan or any combination of two or more of these compounds , with a preference for at least one active compound selected from the group of OPP, Cl-cresol , DCBA and 4-TAP . This is meant to include compositions with one of these four compounds or any combination of two or more of these compounds .

[0032] A single active compound from the above lists may be used in a particular disinfectant composition according to the invention, but it is also very well possible to use a combination of two or more of any of the active compounds as listed above .

[0033] The invention is based on the observation of the inventors that fatty acids having a chain length of 10 to 26 carbon atoms and fatty alcohols having a chain length of 10 to 26 carbon atoms in a disinfectant composition result in a signi ficantly improved biocidal activity against the durable forms , in particular eggs or oocysts , of parasitic organisms .

[0034] In the context of the present invention the disinfectant composition may comprise either fatty acids having a chain length of 10 to 26 carbon atoms ( CI O-26 ) or fatty alcohols having a chain length of 10 to 26 carbon atoms or a combination of such fatty acids or fatty alcohols . Likewise , combinations o f di f ferent fatty acids having a chain length of 10 to 26 carbon atoms and / or di f ferent fatty alcohols having a chain length of 10 to 26 carbon atoms may be used . In a suitable embodiment a single compound may be chosen amongst these fatty acids and fatty alcohols for inclusion in the disinfectant composition .

[0035] In accordance , in one embodiment the disinfectant composition may comprise at least one fatty acid having a chain length of 10 to 26 carbon atoms . With respect to the fatty acids , best results are obtained with fatty acids having a chain length of 10 to 22 carbon atoms . In accordance , it is preferred to use at least one fatty acid having a chain length of 10 to 22 carbon atoms ( C10-C22 ) . Optimal results are obtained with fatty acids having a chain length of 16 to 20 carbon atoms ( C16-C20 ) , in particular 18 carbon atoms ( C18 ) . It is therefore more preferred to use at least one fatty acid having a chain length of 16 to 20 carbon atoms , preferably 18 carbon atoms . With regard to the fatty acids it is preferred to use compounds with unsaturated carbon chains .

[0036] In addition or alternatively, the disinfectant composition may comprise at least one fatty alcohol having a chain length o f 10 to 26 carbon atoms . With respect to the fatty alcohols , best results are obtained with fatty acids having a chain length of 10 to 16 carbon atoms ( CI O- 16 ) . In accordance , it is preferred to use at least one fatty acid having a chain length of 10 to 16 carbon atoms , in particular 12 carbon atoms ( C12 ) .

[0037] With regard to the preparation of stable dispersions , such as stable emulsions it is preferred in respect of the fatty acids to use compounds with unsaturated carbon chains . With regard to the fatty alcohols there is no particular preference in this regard .

[0038] The disinfectant composition may be provided in any suitable form . For instance , solid or semi-solid forms may be suitable for sales and distribution . However, when the composition is used in practice , that is for disinfection of living areas of livestock, the composition should be in liquid form . It is therefore preferred that the disinfectant composition is in the form of a dispersion, such as a solution or emulsion, and, given the presence of fatty acids and / or fatty alcohols , in particular an emulsion . This requires the presence of a solvent .

[0039] In accordance , in a preferred embodiment the disinfectant composition of the invention is in the form of an emulsion and which comprises at least one solvent . Said at least one solvent may suitably be selected from water, ethanol , 2-propanol , 3-methoxy-3-methyl- l-butanol (MMB ) , dimethylsul foxide ( DMSO) , ethyl diglycol (EDG) , butyl diglycol (BDG) , acetone , butyl lactate , benzyl alcohol , phenoxy ethanol , monopropylene glycol , hexyl glycol , as single solvent or as any combination of two or more of these solvents . Particular suitability has been found for EDG as solvent , optionally in combination with water, preferably minor amounts of water . In this respect said at least one solvent may suitably be EDG or EDG in combination with water .

[0040] Because the disinfectant composition may suitably be in the form of an emulsion, the composition may further comprise at least one emulsi fier so as to obtain a homogeneous and stable emulsion . Said at least one emulsi fier may suitably be selected from the group of sodium linear alkylbenzene sul fonate ( LAS ) , polysorbate 80 , alkylglucoside , such as C8-alkyl glucoside .

[0041] The disinfectant composition may contain further additives , such as acids to adj ust the pH, for instance for reasons of stability of the composition .

[0042] Accordingly, the disinfectant composition may comprise at least one acid . Said at least one acid may suitably be selected from the group of propionic acid, acetic acid, formic acid, lactic acid, glycolic acid, phosphoric acid, citric acid, sul furic acid, salicylic acid, benzoic acid, sorbic acid, preferably propionic acid or lactic acid, more preferably lactic acid . Some of these acids may exert keratolytic action, which may be acceptable and even desirable under certain conditions. This however does not compromise the advantageous effects on penetration into the durable forms of parasites due to the fatty acids and / or fatty alcohols as specified above. In fact, these keratolytic acids may have an advantageous additive effect.

[0043] Further additives may be included, for instance for improving odor and to conceal the often unpleasant smell of the biocidal active compounds in the composition. In this respect, the disinfectant composition may suitably comprise at least one natural or essential oil. Said at least one natural or essential oil may suitably be selected from tea tree oil, lavender oil, limonene, thymol, carvacrol, menthol, cinnamaldehyde, eugenol, eucalyptol, or any combination two or more thereof.

[0044] In accordance with the invention a weight ratio of the abovementioned at least one active compound to said at least one compound selected from fatty acids and fatty alcohols in the disinfectant composition is in the weight range of 1:10 to 6:1, or in other words a weight ratio of [active compound(s) ] / [fatty acid(s) and / or fatty alcohol (s) ] of 0.1 to 6.

[0045] In preferred embodiments a preferred weight ratio of [active compound(s) ] / [fatty acid(s) and / or fatty alcohol (s) ] is 0.1 to 5.

[0046] More preferably the weight ratio of [active compound (s) ] to [fatty acid(s) and / or fatty alcohol (s) ] is 0.5 to 5.

[0047] Even more preferably the weight ratio of [active compound(s) ] / [fatty acid(s) and / or fatty alcohol (s) ] is 0.5 to 3.

[0048] Even more preferably the weight ratio of [active compound(s) ] / [fatty acid(s) and / or fatty alcohol (s) ] is 0.5 to 1.5. In a particular preferred exemplary embodiment the weight ratio of [active compound(s) ] / [fatty acid(s) and / or fatty alcohol (s) ] is about 0.75.

[0049] Suitable disinfectant compositions may have the following formulation:

[0050] • 5-30 % of said at least one active compound;

[0051] • 5-50 % of said at least one acid;

[0052] • 5-50 % of said at least one compound selected from fatty acids and fatty alcohols;

[0053] • 5-50 % of said at least one emulsifier;

[0054] • 0-20 % of said at least one natural / essential oil;

[0055] • 0-40 % of said at least one solvent, preferably

[0056] • 10-25 wt% of said at least one active compound;

[0057] • 10-30 wt% of said at least one acid;

[0058] • 10-20 wt% of said at least one compound selected from fatty acids and fatty alcohols;

[0059] • 10-20 wt% of said at least one emulsifier;

[0060] • 0-15 wt% of said at least one natural / essential oil;

[0061] • 10-30 wt% of said at least one solvent, wherein all percentages are based on the weight of the composition. Such compositions have the advantages of being stable and concentrated, which is advantageous in terms of storage and distribution. In accordance, these formulations essentially are disinfection composition concentrates.

[0062] In the context of the invention, the disinfection composition relates to concentrates as well as dilutions thereof .

[0063] The invention also relates to a method for disinfecting at least one area to prevent parasitosis, in particular parasitosis by the parasitic organisms mentioned above. This is in particular achieved by killing durable forms of parasites . In this respect it is to be understood that the method of the invention may also be defined as a method for killing durable forms of parasites , in particular the parasites as described and speci fied above .

[0064] The invention is particularly useful for disinfecting areas intended for animal housing, transport or for any permanent or temporary animal presence .

[0065] The areas will in particular relate to a surface of a contained area where animals are intended to be kept . In accordance with the above , these animals in particular may be livestock, in particular poultry, pigs or cattle . Given the large problems with Eimeria in poultry, such as chickens , the invention is very useful in poultry living areas , in particular the living areas of chickens . Further, given the large problems with Cryptosporidi um with pigs and cattle , the invention is very useful in the living areas of pigs and cattle .

[0066] In accordance with the above the areas to be disinfected in the context of the invention in particular relate to means of animal transport and temporary or permanent animal housing, and in particular the surfaces thereof , including but not limited to stables , stalls , pens , houses . Surfaces in this context may include without limitation floors , floor coverings , partition surfaces , walls , wall cracks or j oints , etc . , or any materials intended for use in the presence of animals and which require disinfection, such as stable equipment including but not limited to feed troughs and drinking lines . Said area may suitably relate to an area that is not a surface of a living human or animal being .

[0067] The invention encompasses disinfecting said area with an ef fective amount of the abovementioned disinfectant composition. In general, said area(s) are disinfected in the absence of the animals.

[0068] In order to be applied the disinfection composition concentrates with the formulations specified above may be diluted in water or any other suitable solvent system for use in disinfecting surfaces of living areas of livestock, for instance to obtain 0,5-10 % w / v dilutions, suitably a 100 x dilution (1% w / v) in water. The inventors have found that the disinfectant composition according to the invention allows for a 5.3 to 8.3 times higher dilution of the biocidal compounds than the compositions mentioned in abovementioned US patent 5, 620, 655 and Australian patent application AU 2012241180 Al showing the improved efficacy of the compositions of the invention.

[0069] The dilutions may be applied to the living areas by any suitable means of application, for instance by spraying, fogging, misting, foaming and / or wiping.

[0070] Examples

[0071] The following examples are shown to illustrate and support the principle of the present invention and not to limit the scope of the claims.

[0072] Preparation of oocysts

[0073] Eimeria spp oocysts were harvested from fresh chicken manure. The manure was washed and centrifuged multiple times with brine. The purified oocysts were stored and refrigerated, so that no further sporulation could take place .

[0074] Formulations of disinfectant agent Formulations were prepared by dissolving the active ingredients in the solvent and acid. After dissolution, the rest of the ingredients were added stepwise while mixing.

[0075] Testing

[0076] A predetermined amount of oocysts was mixed with a predetermined amount and concentration of the formulation. After a predetermined exposure time, the oocysts were separated from the formulation by washing and centrifuging and evaluated.

[0077] Evaluation

[0078] The destructive effect of the formulations on oocysts was evaluated with microscopy. After exposure of the oocysts to the formulation during a predetermined time, the solution was put in a MacMaster counting chamber for evaluation. The oocysts were scored visually as shown in Fig.l and divided into four groups; A: no change in structure of the oocyst observed (see Fig. 1A) , B: the cell-wall is intact, however, the internal structure of the oocyst is lost (see Fig. IB) , C: cell wall is damaged and the cell is leaking (see Fig.lC) , D: cell wall is completely broken and the interior components have leaked out substantially (see Fig. ID) .

[0079] Resul ts

[0080] Various biocidal agents

[0081] Table 1 below shows the results of tests with disinfectant compositions with various biocidal active compounds in combination with a C18-fatty acid. Percentages of compounds are given as weight percentages based on the total weight of the compositions. Dosage: 1 % of the compositions shown in Table 1, exposure time: 2 hours, at room temperature. Dosage percentages in the present examples section are based on weight percentages and dilutions are in water .

[0082] The kill score was calculated on the total number of oocysts recovered that were either falling under groups A and B, wherein these oocysts scored as surviving or potentially alive . The experiments assumed 100 oocysts per lane that are minimally recovered in the blank experiments .

[0083] It is noted in this regard that the actual percentage of killed or irreversibly damaged oocysts is higher than the kill score in the tables below, because also oocysts scored under B would highly likely be irreversibly damaged or dead . It was however chosen to score these under " intact" to avoid false scores of dead Eimeria oocysts .

[0084] Table 1: various biocidal active agents (* lactic acid is added as an 88% aqueous solution)

[0085] Table 1 shows a significantly higher kill score with Examples 1A-D that contain a C18-fatty acid compared to Comparative example IE that does not contain a fatty acid. A particular reference is made to Example 1A with a kill score of 96% and Comparative Example with a dramatically lower kill score of 22%. Various fatty acids

[0086] Table 2 below shows the results of tests with several fatty acids. The C18 and C22 fatty acids are unsaturated fatty acids, the rest are saturated fatty acids. Percentages of compounds are given as weight percentages based on the total weight of the compositions. The kill score was calculated as described for the results in Table 1.

[0087] Dosage: 1% of the compositions shown in Table 2, exposure time: 2 hours, at room temperature.

[0088] Table 2 : fatty acids with various chain lengths (* lactic acid is added as an 88% aqueous solution)

[0089] Table 2 shows that all tested compositions of Examples 2A-2D have signi ficantly higher kill counts than comparative example IE , showing that the advantageous ef fect of improvement of ef fectivity of the disinfectant compositions is achieved over a broad range of fatty acid chain lengths . Best results are obtained with a C18- fatty acid . Vari ous fa t ty al cohol s

[0090] Table 3 below shows the results of tests with several fatty alcohols ( all saturated) . Percentages of compounds are given as weight percentages based on the total weight of the compositions . The kill score was calculated as described for the results in Table 1 . Dosage: 1% of the compositions shown in Table 2, exposure time: 2 hours, at room temperature. lactic acid is added as an 88% aqueous solution)

[0091] Table 3 shows that all tested compositions of Examples

[0092] 3A-3D have significantly higher kill scores than comparative example IE, showing that the advantageous effect of improvement of efficacy of the disinfectant compositions is achieved over a broad range of fatty alcohol chain lengths. Best results are obtained with a C12-fatty alcohol.

[0093] Comparative examples

[0094] Two compositions as disclosed in US 3,932, 684 were also tested as comparative examples. The test was performed in accordance with the procedure as set out above in relation to the compositions according to the invention.

[0095] Specifically, composition A of 10 parts by weight of p-chloro-m-cresol (Cl-cresol) , 10 parts by weight of perchloroethylene, 10 parts by weight of an aqueous 50% solution of the surface-active compound sodium alkylbenzene sulfonate having 8 to 20 carbon atoms in the alkyl and 15 parts by weight of isopropanol was tested in a 1%, 2% and 4% aqueous dilution. This translates to end-concentrations of Cl-cresol of 0.1%, 0.2% and 0.4%.

[0096] Also composition B of 15 parts by weight of o-phenyl- phenol (OPP) , 10 parts by weight of perchloroethylene, 10 parts by weight of an aqueous 50% solution of the surfaceactive compound sodium alkylbenzene sulfonate having 8 to 20 carbon atoms in the alkyl, 30 parts by weight of isopropanol, and 35 parts by weight of distilled water was tested in a 1%, 2% and 4% aqueous dilution. This translates to end-concentrations of OPP of 0.15%, 0.3% and 0.6%.

[0097] Kill scores are shown in Table 4 below:

[0098] Table 4: kill scores (%) of comparative compositions A and B The results in Table 4 show that under the same experimental conditions the compositions according to the invention are much more effective than the comparative examples (compositions A and B) . Namely, even with low end- concentrations of 0.15% OPP or Cl-cresol very high kill scores of more than 90% were achieved with the compositions according to the invention, while the compositions of the comparative examples show much lower kill scores (8,86 - 23,38%) with higher end-concentrations of OPP and Cl-cresol of up to 0, 6%.

Claims

CLAIMS1. Disinfectant composition comprising- at least one active compound selected from the group of orthophenylphenol (OPP) , chloro-cresol (Cl-cresol) , dichlorobenzyl alcohol (DCBA) and 4-t-amylphenol (4-TAP) ; and- at least one compound selected from fatty acids or fatty alcohols and which have a chain length of 10 to 26 carbon atoms; wherein a weight ratio of said at least one active compound to said at least one compound selected from fatty acids and fatty alcohols is in the weight range of 1:10 to 6:1.

2. Disinfectant composition according to claim 1, comprising at least one fatty acid having a chain length of 10 to 26 carbon atoms.

3. Disinfectant composition according to claim 2, comprising at least one fatty acid having a chain length of 10 to 22 carbon atoms.

4. Disinfectant composition according to any of the claims 1 to 3, comprising at least one fatty acid having a chain length of 16 to 20 carbon atoms, preferably 18 carbon atoms .

5. Disinfectant composition according to any of the previous claims, comprising at least one fatty alcohol having a chain length of 10 to 26 carbon atoms.

6. Disinfectant composition according to claim 7, comprising at least one fatty alcohol having a chain length of 10 to 16 carbon atoms, preferably 12 carbon atoms.

7. Disinfectant composition according to any of the previous claims, which is in the form of an emulsion and which comprises at least one solvent.

8. Disinfectant composition according to claim 7, wherein said at least one solvent is selected from water, ethanol, 2-propanol, 3-methoxy-3-methyl-l-butanol (MMB) , dimethylsulfoxide (DMSO) , ethyl diglycol (EDG) , butyl diglycol (BDG) , acetone, butyl lactate, benzyl alcohol, phenoxy ethanol, monopropylene glycol, hexyl glycol, as single solvent or as any combination of two or more of these solvents .

9. Disinfectant composition according to claim 8, wherein said at least one solvent is EDG or EDG in combination with water.

10. Disinfectant composition according to any of the claims 7 to 9, further comprising at least one emulsifier.

11. Disinfectant composition according to claim 10, wherein said at least one emulsifier is selected from the group of sodium linear alkylbenzene sulfonate (LAS) , polysorbate 80, alkylglucoside, such as C8-alkyl glucoside.

12. Disinfectant composition according to any of the claims 7 to 11, further comprising at least one acid.

13. Disinfectant composition according to claim 12, wherein at least one acid is selected from the group of propionic acid, acetic acid, formic acid, lactic acid, glycolic acid, phosphoric acid, citric acid, sulfuric acid, salicylic acid, benzoic acid, sorbic acid, preferably propionic acid or lactic acid, more preferably lactic acid.

14. Disinfectant composition according to any of the claims 7 to 13, further comprising at least one natural or essential oil.

15. Disinfectant composition according to claim 14, wherein at least one natural or essential oil is selected from tea tree oil, lavender oil, limonene, thymol, carvacrol, menthol, cinnamaldehyde, eugenol, eucalyptol, or any combination two or more thereof.

16. Disinfectant composition according to any of the previous claims, with the following formulation:5-30 wt% of said at least one active compound;5-50 wt% of said at least one acid;5-50 wt% of said at least one compound selected from fatty acids and fatty alcohols;5-50 wt% of said at least one emulsifier;0-20 wt% of said at least one natural / essential oil;0-40 wt% of said at least one solvent.

17. Disinfectant composition according to claim 16, with the following formulation10-25 wt% of said at least one active compound;10-30 wt% of said at least one acid;10-20 wt% of said at least one compound selected from fatty acids and fatty alcohols;10-20 wt% of said at least one emulsifier;0-15 wt% of said at least one natural / essential oil;10-30 wt% of said at least one solvent.

18. A method for disinfecting at least one area to prevent parasitosis, wherein the method comprises- disinfecting said area with an effective amount of a disinfectant composition according to any of the previous claims.

19. Method according to claim 18, wherein said parasitic organisms are durable forms thereof, in particular eggs or oocysts thereof.

20. Method according to claim 19 or 20, wherein the parasitic organism is selected from:Eimeria , preferably Eimeria tenella, in particular in the form of oocysts thereof;Cryptosporidium, in particular in the form of oocysts thereof;Ascaris , preferably Ascaris suum, in particular in the form of eggs thereof;Cystoisospora suis, in particular in the form of eggs thereof .

21. Method according to any of the claims 19 to 20, wherein said area is for animal housing, transport or for any permanent or temporary animal presence.

22. Method according to any of the claims 18 to 21, wherein animals are livestock, in particular poultry, pigs or cattle.

23. Use of at least one compound selected from fatty acids and fatty alcohols and which have a chain length of 10 to 26 carbon atoms in a disinfectant composition for improving penetration of at least one active compound selected from the group of OPP, Cl-cresol, DCBA and 4-TAP and contained in said composition into the durable forms, in particular eggs or oocysts, of parasitic organisms.

24. Use according to claim 23, wherein said at least one fatty acid or fatty alcohol having a chain length of 10 to 26 carbon atoms are contained in a disinfectant composition according to any of the claims 1 to 19.

25. Use according to claim 23 or 24, wherein the parasitic organism is selected from:Eimeria species , in particular in the form of oocysts thereof;Cryptosporidium, in particular in the form of oocysts thereof;Ascaris , in particular Ascaris suum, in particular in the form of eggs thereof;Cystoisospora suis, in particular in the form of eggs thereof .

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