Method for constructing bone organoid and use thereof

By adding phosphorus and calcium sources to the osteogenic induction medium for in vitro culture of bone organoids, and co-culturing them with cells using hydrogel microcarriers, the problems of long bone repair cycles and limited material sources were solved, achieving rapid mineralization and efficient repair of bone organoids.

WO2026114438A1PCT designated stage Publication Date: 2026-06-04BEIJING TISSHUE MEDICAL DEVICES CO LTD +1
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
BEIJING TISSHUE MEDICAL DEVICES CO LTD
Filing Date
2026-01-15
Publication Date
2026-06-04

AI Technical Summary

Technical Problem

Existing bone repair technologies suffer from problems such as long repair cycles, limited material sources, high risk of rejection, and a mismatch between the degradation rate of scaffold materials and the growth rate of bone tissue. In particular, in bone tissue engineering, the mineralization process of bone organoids needs to be accelerated.

Method used

In the in vitro culture of bone organoids, osteogenic induction culture medium with the addition of additional phosphorus and calcium sources promotes the mineralization process of stem cells. By using hydrogel microcarriers to co-culture with cells or cell-free microcarriers, the phosphorus and calcium sources can act separately or together in the gel system or culture medium to achieve rapid mineralization of bone organoids.

Benefits of technology

It significantly shortens the bone repair cycle, increases the maturation speed of bone organoids, reduces the use of biologically derived materials, reduces the risk of rejection, and provides a more efficient bone repair solution.

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Abstract

Provided are a hydrogel microsphere for constructing a bone organoid, a kit, a bone organoid, a bone organoid-loaded hydrogel, and a preparation method therefor and the use thereof. The kit comprises (1) a gel system and / or (2) a culture medium system, and further comprises (3) a phosphorus source and / or a calcium source, wherein an addition amount of the phosphorus source in the gel system or culture medium system is 24000-33000 mg / L; and an addition amount of the calcium source in the culture medium system is 2-8 mmol / L. The calcium source and the phosphorus source required for mineralization are introduced into a hydrogel and a culture matrix during the in vitro culture of bone organoids. Without external assistance, only a single cell type is used, along with simple ionic calcium and phosphorus, and microspheres are used to regulate the spatial distribution of calcium and phosphorus, which is beneficial to promoting cellular mineralization and osteogenesis. The in-situ mineralization method can effectively guide osteogenesis, mineralization, cell layer formation, shaping and remodeling, promote the development of bone organoids, effectively shorten the bone repair cycle, and achieve greater safety and broader applicability.
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