Thermostable bioluminescent probes for integrated DNA detection and amplification

Thermostable bioluminescent probes with DNA-binding domains allow real-time DNA detection during isothermal amplification, addressing stability and equipment limitations, enabling sensitive and specific point-of-care diagnostics.

WO2026117146A1PCT designated stage Publication Date: 2026-06-04TECH UNIV EINDHOVEN
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
TECH UNIV EINDHOVEN
Filing Date
2025-11-26
Publication Date
2026-06-04

AI Technical Summary

Technical Problem

Existing DNA detection methods, such as PCR and LAMP, require complex equipment or suffer from high background signals and matrix-dependent properties, limiting their use in point-of-care diagnostics, while bioluminescent sensors like LUMID lack stability at LAMP temperatures, necessitating post-amplification reaction transfer.

Method used

Development of thermostable bioluminescent probes covalently linked to thermostable DNA-binding protein domains for real-time DNA detection during isothermal amplification, utilizing bioluminescence resonance energy transfer (BRET) for a ratiometric readout.

Benefits of technology

Enables real-time, sensitive, and specific DNA detection in complex samples with a simple camera-based readout, suitable for low-resource settings, without the need for specialized equipment.

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Abstract

This invention pertains in general to probes for detecting and / or quantifying double-stranded DNA. The invention particularly relates to luminescence based probes, and methods of using such probes to detect or quantify double-stranded DNA, and in particular their use in real time DNA amplification and quantification reactions. The invention further describes compositions including the probes suitable for the described uses and methods.
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