CAS9 mutants with improved activity with truncated pegrna in prime editing applications
Mutated Cas9 variants with specific mutations enhance prime editing activity with truncated pegRNAs, addressing synthesis challenges and improving editing efficiency and cargo capacity in prime editing technologies.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- INTEGRATED DNA TECHNOLOGIES INC
- Filing Date
- 2025-12-16
- Publication Date
- 2026-06-25
AI Technical Summary
Existing prime editing technologies face limitations with full-length pegRNAs, which are difficult to chemically synthesize and limit cargo capacity, necessitating the development of more potent Cas9 variants that can function with truncated pegRNAs for enhanced editing efficiency.
Development of prime editor variants with specific mutations in the Cas9 protein, such as E1071R, E1071K, T1065R, N758Y, A1034E, D180H, H420Q, Q894A, R40L, I122P, R215L, K961E, E1007L, K31Y, D364S, K382S, R457S, S42E, N193M, K649N, Q1364S, and F1008V, allowing them to bind to truncated pegRNAs with enhanced editing activity.
The mutated Cas9 variants exhibit at least 5-30% increased prime editing activity with truncated pegRNAs, facilitating easier synthesis and larger cargo capacities while maintaining precise gene editing capabilities.
Smart Images
Figure US2025059796_25062026_PF_FP_ABST