Stable synergistic agrochemical composition

Abstract

The present invention relates to a stable synergistic agrochemical composition comprising plant growth regulators for synergistic and effective plant growth regulation, activation, stimulation and plants / crops yield. The invention results in higher yield, better quality produce, reduced fertilizer use, and stable non-phytotoxic formulations. Further, the invention also relates to process of preparation of stable synergistic agrochemical composition.

Description

[0001] STABLE SYNERGISTIC AGROCHEMICAL COMPOSITION FIELD OF THE INVENTION

[0002] The present invention relates to a stable synergistic agrochemical composition comprising A) Brassinolide or its agrochemically acceptable salts, esters and derivatives; B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) a bio-effective amount of Sulphur. The stable synergistic agrochemical composition is effective for plant growth regulation, activation and stimulation. The present invention also relates to a process for preparation of such stable synergistic agrochemical composition for synergistic and effective plant growth regulation, activation, and stimulation.

[0003] BACKGROUND OF THE INVENTION

[0004] Plant growth regulators, plant stimulators and plant activators are transmitted to the site of action in plants and promote or inhibit certain aspects of their life processes at very low concentrations to meet agricultural and horticultural requirements. Depending on the crop species, growth stages, and application objective, such agents regulate a wide range of plant functions, for example: dormancy; promoting cell growth and division; plant height, promoting rooting; controlling germination controlling lateral buds or tillers; controlling plant type, such as stunting and lodging; controlling flowering or Male and female sex, induce no fruit; flower thinning, number of flowers, control fruit drop; control fruit shape or maturity; enhance stress resistance, such as disease resistance, drought resistance, salt tolerance, antifreeze; enhance absorption of fertilizer; increase sugar or change acidity; improve fragrance and color; promote latex or resin secretion; defoliation or evasion, preservation, etc.

[0005] Various plant growth regulators, activators, and stimulators have long been known and employed for influencing plant growth and development. One of the plant growth activators is Brassinolide, which is also referred as plant hormone. Brassinolide is oligodynamic in nature and affects specific target tissues that are sensitive to the plant hormone such as but not limited to indole-3-acetic-acid (IAA). It is a biologically active steroidal lactone first isolated from rape pollen that stimulates growth of green plant tissues. It promotes cell division; promote vascular differentiation; protects plants during cold and drought stress; promotes resistance to salt stress; increases the metabolism of plants. Brassinolide is also known to improve pollen tube growth and showed increasing and decreasing effect on pollen viability and germination.It is one of the most effective plant growth regulators, activator, stimulator, whose function is to encourage growth, yield and resist various stresses facing plants.

[0006] Another known plant growth regulator, is Triacontan-l-ol that also works as a biostimulant. It is a natural chemical that acts as a phytohormone which is used in commercial horticulture as a plant growth regulator including but not limited to ornamental plants including roses; Cotton; Tomato; Chilli peppers; Rice; Peanuts; Potato, etc. It effects photosynthesis, plant metabolism, and increase the growth of roots, shoots and flower production and influences nutrient uptake. In addition to plant growth regulators, sulphur plays an important dual role as a secondary plant nutrient and is indispensable for amino acid and protein synthesis, chlorophyll formation, and enzymatic activity. Sulphur also enhances phosphorus uptake in the root zone and, in synergy with phosphorus, facilitates the absorption of micronutrients such as zinc, iron, and manganese. Plants deficient in sulphur exhibit poor utilization of aforementioned primary nutrients such as nitrogen, phosphorus and potassium, resulting in and a significant reduction in enzymatic and metabolic activities at all stages of plant growth, which has become a significant concern for farmers. Beyond its nutritional role, sulphur improves soil structure, supports microbial activity, and maintains pH balance, thereby contributing to long-term soil fertility. It also improves tolerance to abiotic stresses by supporting metabolic activity under adverse conditions. Similar to plant growth regulators, the effect of sulphur is dependent on the dose and rate of application, wherein deficiency adversely affects protein metabolism and chlorophyll formation ultimately affecting growth parameters such as plant height and number of flowers, etc, while excessive application may lead to soil acidification and nutrient imbalance. Under sulphur-deficient conditions, plants may exhibit excessive vegetative elongation, increased stem or neck thickness, and inadequate development of storage or reproductive organs, which is attributed to inefficient translocation of assimilates within the plant. Additionally, an important agronomic advantage of sulphur is its residual availability, as applied sulphur can remain in the soil and be subsequently utilized by following crops. This residual effect allows sulphur to provide benefits beyond a single cropping season and supports more sustainable crop rotation practices. There are various prior arts known that discloses a composition comprising plant growth regulator and plant activator with inclusion of trace elements, however, synergistic effect as attributed by the composition of plant growth regulator and plant activator with sulphur is not specifically known in the art. Moreover, there still exists a need for an alternative synergistic composition in optimum quantities / efficacious amounts / weight % range, comprising the aforementioned actives in the composition.In view of the existing needs, the present invention discloses a stable synergistic agrochemical composition comprising plant growth regulators such as Brassinolide and Triacontan-l-ol which are known to influence physiological processes including cell division, photosynthesis, directly affecting overall plant growth parameters such as plant height, number of flowers, number of leaves, etc. The composition of present invention also combines essential nutrient i.e. sulphur which plays a critical role in supporting nutrient assimilation and metabolic functions. However, when these components are applied individually, their effectiveness may be limited by physiological imbalances, sub-optimal nutrient utilization, or inefficient translocation of assimilates within the plant system.

[0007] Brassinolide primarily functions as a plant growth regulator that modulates growth responses and developmental processes, whereas triacontan-l-ol acts as a growth activator that enhances metabolic efficiency and photosynthetic activity. Sulphur, on the other hand, serves as an essential secondary nutrient that supports enzymatic activity and facilitates effective utilization of major nutrients. In the absence of adequate sulphur, plants often exhibit imbalanced vegetative growth and poor translocation of assimilates, which can reduce the effectiveness of applied growth regulators.

[0008] References can be made to CN103563986A, which discloses a plant growth regulating composition comprising active ingredient A and active ingredient B in a ratio of 70:1-1:300. Active ingredient A is triacontan-l-ol and active ingredient B is selected from the group consisting of benzylaminopurine, brassinolide, amine fresh ester, and humic acid.

[0009] Further, CN104026127A also discloses a plant growth regulator composition comprising diethyl aminoethyl hexanoate and triacontan-l-ol.

[0010] Thus, there arise a need to develop a stable synergistic agrochemical composition with a plant growth regulator and plant activator with bio-effective amount of sulphur including sulphur in all its forms which are compatible with each other in terms of stability and ensures enhanced bio-efficacy and synergistic effect.

[0011] It has been surprisingly found that by virtue of the composition of present invention, the plant growth regulation, activation, stimulation of brassinolide can be significantly enhanced whencombined with plant growth regulator especially triaconta-l-ol along with bio-effective amount of sulphur.

[0012] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art. The present invention composition is synergistic, efficacious, environmentally friendly, simple to formulate, stable and is safe to use.

[0013] Therefore, present invention aims to overcome the problems of the prior arts and provide a stable synergistic agrochemical composition comprising A) Brassinolide or its agrochemically acceptable salts, esters and derivatives; B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) a bio-effective amount of Sulphur, for synergistic and effective plant growth regulation, activation, and stimulation.

[0014] The present invention also discloses a process of preparation of such stable synergistic agrochemical composition for synergistic and effective plant growth regulation, activation, and stimulation.

[0015] OBJECTIVES AND ADVANTAGES OF THE INVENTION

[0016] The principal objective of the present invention is to provide a novel and effective stable synergistic agrochemical composition demonstrating high efficacy and to provide improved plant growth regulation, activation, stimulation and enhancement of plant growth parameters such as but not limited to plant height, number of flowers, number of leaves etc.

[0017] Another objective of the present invention is to provide a cost-effective agrochemical composition that maximizes return on investment for farmers by improving crop yield and quality at reduced input levels, while simultaneously contributing to environmental protection and long-term agricultural sustainability.

[0018] Another objective of the present invention is to provide a novel and effective stable synergistic agrochemical composition which is synergistic, and shows an improved plant growth regulation, activation, stimulation at reduced dose.

[0019] Accordingly, the main objective of the present invention is to provide a stable synergistic agrochemical composition for increasing plant growth by providing better tolerance to stress factors.It is another objective of the present invention to provide improved, stable and ready to use agrochemical composition that simplifies agricultural practice by enabling one shot application for multiple benefits, thus saving labour and ensuring consistent and reliable performance across a wide range of crops as compared to prior art formulations or mixtures or compositions. It is another objective of the present invention to provide a novel and effective stable synergistic agrochemical composition demonstrating high efficacy, high selectivity and agricultural sustainability.

[0020] Another object of the invention is to provide a composition with improved technological attributes including enhanced penetration capacity, spreading and coverage effect, flowability, permeability, and storage stability, achieved through the inclusion of suitable additives and / or adjuvants.

[0021] It is another objective of the present invention is to provide a composition and method for promoting plant growth and regulation / development in plants, crops and plant propagation materials.

[0022] It is another objective of the present invention is to provide one shot application of novel and effective stable synergistic agrochemical composition for promoting growth and development of crops, plants, sapling, etc at lower application rates and is thus, environmentally safe, possesses broad spectrum bio-efficacy, is non-phy to toxic.

[0023] It is another objective of the present invention to provide a novel and effective stable synergistic agrochemical composition, which has improved stability during the desired shelflife at various temperature and pH conditions and has an enhanced efficacy, penetration capacity and is cost-effective and economically more beneficial.

[0024] It is another objective of the present invention to provide a novel and effective stable synergistic agrochemical composition which further comprises additives and / or adjuvants, for providing the enhanced stability, enhanced permeability, improved penetration capacity, uniform sprayability, improved rain-fastness, enriched biotic factors, better flowability, enhanced spreading or coverage effect, effective storage stability, longer residual effect, and safer to the environment attributes to the composition of present invention.Another objective of the present invention is to provide a stable synergistic agrochemical composition comprising A) Brassinolide or its agrochemically acceptable salts, esters and derivatives; B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) a bio-effective amount of Sulphur.

[0025] Yet another objective of the present invention is to provide a process for the preparation of a stable synergistic agrochemical composition comprising A) Brassinolide or its agrochemically acceptable salts, esters and derivatives; B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) a bio-effective amount of Sulphur.

[0026] Some or all these and other objects of the invention can be achieved by way of the invention described hereinafter.

[0027] ADVANTAGES:

[0028] 1. The stable synergistic agrochemical composition of the present invention resulted in significant plant growth, regulation, activation, stimulation and development in various crops such as Fruit including apples; pears, citrus; Rice; Wheat; Corn; Tomatoes: Cucumber; Lettuce, etc, as compared to the other treatments and untreated check. 2. The stable synergistic composition of the present invention is non-phytotoxic and help in increasing plant growth by providing better tolerance to stress factors. The compositions also help in increasing the yield of crops.

[0029] 3. The stable synergistic agrochemical composition of the present invention is significantly superior and synergistic from its individual products / compositions and respective binary compositions.

[0030] 4. The stable synergistic agrochemical composition of the present invention ensures improved agronomical factors including but not limited to plant height, number of flowers, number of leaves etc. attributing to higher yields as compared to the untreated control and binary and solo treatments applied in the various crops. Also found economical with higher Cost: Benefit Ratio and shows no phytotoxicity effect on various crops including but not limited to tomato.

[0031] 5. The stable synergistic agrochemical composition of the present invention promotes plant health and increases plant yield in the field and shows to has an improved rain fastness.

[0032] 6. The stable synergistic agrochemical composition of the present invention stimulates growth of plant tissues, promotes plant growth by increasing photosynthetic rate,nitrogen fixation, water, and nutrient uptake, stomata conductance, and gas exchange therefore, resulting into improved photosynthesis, plant metabolism, and so can increase biomass, flower production by influencing nutrient uptake.

[0033] SUMMARY OF THE INVENTION

[0034] Accordingly, the main aspect of the present invention is to provide a stable synergistic agrochemical composition exhibiting improved plant growth regulation, activation, and stimulation.

[0035] In an aspect, the present invention provides a stable synergistic agrochemical composition comprising:

[0036] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0037] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) Bio-effective amount of sulphur.

[0038] In another aspect, the present invention provides a stable synergistic agrochemical composition comprising:

[0039] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0040] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0041] C) Bio-effective amount of sulphur; and

[0042] D) One or more agrochemically acceptable excipients.

[0043] In another aspect, the present invention provides a stable synergistic agrochemical composition comprising:

[0044] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0045] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0046] C) Bio-effective amount of Sulphur; and

[0047] D) At least one or more additives.

[0048] In one another aspect, the present invention provides a stable synergistic agrochemical composition comprising:

[0049] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0050] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0051] C) Bio-effective amount of sulphur;D) At least one or more additives selected from Urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and Epoxide soybean oil either alone or in combination thereof; and

[0052] E) One or more agrochemically acceptable excipients.

[0053] In one another aspect, the present invention provides a stable synergistic agrochemical composition comprising:

[0054] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0055] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; and

[0056] C) Bio-effective amount of sulphur, in the range of 0.001-10% by weight of the composition.

[0057] In yet another aspect, the present invention provides a process for preparation of the stable synergistic agrochemical composition comprising:

[0058] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0059] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) Bio-effective amount of sulphur.

[0060] In another aspect of the present invention, the present stable synergistic agrochemical composition is stable and has enhanced shelf life.

[0061] In an aspect of the present invention, the present invention provides a stable synergistic agrochemical composition which further comprises additives and / or adjuvants, for providing the following attributes to the composition of present invention:

[0062] a) enhanced stability,

[0063] b) enhanced permeability,

[0064] c) improved penetration capacity,

[0065] d) uniform spray-ability,

[0066] e) improved rain-fastness,

[0067] f) enriched biotic factors,

[0068] g) better flowability,

[0069] h) enhanced spreading or coverage effect,

[0070] i) effective storage stability,j) longer residual effect,

[0071] k) improved uptake of active ingredients, and

[0072] l) safer to the environment.

[0073] In another aspect, the present invention provides a stable synergistic agrochemical composition for promoting plant growth and development in field crops.

[0074] In an aspect, the present invention provides a synergistic agrochemical composition which has enhanced efficacy and penetration capacity.

[0075] DETAILED DESCRIPTION OF THE INVENTION

[0076] The present invention relates to a stable synergistic agrochemical composition comprising A) Brassinolide or its agrochemically acceptable salts, esters and derivatives; B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) Bio-effective amount of sulphur. Wherein sulphur comprises all agrochemically acceptable forms, derivatives, complexes, and formulations thereof, in efficacious amounts / weight % range, for promoting plant growth, enhancing nutrient uptake, improving stress tolerance, and increasing crop yield and quality. The stable synergistic agrochemical composition is particularly suitable for application in a wide range of crops, including but not limited to fruits such as apples; pears, citrus; Rice; Wheat; Corn; Tomatoes: Cucumber; Lettuce, etc.

[0077] In describing the embodiments of the invention, specific terminology is resorted for sake of clarity. However, it is not intended that the invention be limited to specific terms so selected and it is to be understood that each specific term includes all technical equivalents that operate in a similar manner to accomplish a similar purpose. The terminology used herein is solely for describing particular embodiments and shall not limit the scope of the invention. Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods. All technical and scientific terms used herein have meanings commonly understood by one skilled in the art of agrochemical formulations, plant growth regulation, and crop protection.

[0078] Terms such as “a,” “an,” and “the” may include plural forms unless the context clearly dictates otherwise. The term “or” includes “and / or” unless explicitly stated.

[0079] The transitional phrases “comprise / comprises,” “comprising,” “including,” and “having” are intended to be open-ended, specifying the presence of stated components or steps without precluding additional features, components, or operations.Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.

[0080] The expression of various quantities in terms of “% w / w” or “%” means the percentage by weight, relative to the weight of the total solution or composition, the term “% w / v” means the percentage by weight, relative to the volume of the total solution or composition unless otherwise specified.

[0081] The term “active ingredient” (a.i.) or “active agent” or “active” used herein refers to that component of the composition responsible for control of various growth factors in plants or crops.

[0082] The term “crop” shall include a multitude of desired crop or plants or an individual crop or plant growing at a locus.

[0083] The term “plant growth regulator (PGR)” refers to organic substance, other than nutrients, which influences the physiological processes of plants or crops.

[0084] The term “plant growth stimulator” encompasses compounds, mixtures, or formulations capable of modulating plant metabolic activity, including but not limited to cell division, photosynthesis, nutrient uptake efficiency, flowering, fruit set, biomass accumulation, yield enhancement, stress tolerance, or recovery from abiotic or biotic stress, when applied at biologically effective but non-phytotoxic amounts.

[0085] A bio-effective amount is an amount that produces a statistically or agronomically significant improvement in at least one growth-related parameter selected from, but not limited to, germination, root or shoot development, plant height, number of flowers, biomass accumulation, nutrient uptake efficiency, flowering, fruit set, yield, or stress tolerance, when compared to an untreated control or standard practice.

[0086] The term ‘agrochemically effective amount’ refers to the quantity of the active ingredient(s) that is sufficient to produce the intended beneficial effect in plants, parts of plants, or their loci,including but not limited to stimulation of growth, regulation of development, enhancement of yield, improvement of quality, or protection against biotic or abiotic stress, without causing phytotoxicity or undesirable side effects. The actual agrochemically effective amount may vary depending on factors such as the nature of the active ingredient, type of crop, stage of plant growth, environmental conditions, and method of application.”

[0087] The term “synergistic” refers to the combined effect of the actives, which is greater than the sum of their individual effects, resulting in enhanced plant growth, nutrient uptake, stress tolerance, and yield improvement.

[0088] The term “Sulphur, comprising elemental sulphur and all agrochemically acceptable forms, derivatives, complexes, and formulations thereof’ refers to the sulphur component used in the present invention that may be provided in any agrochemically acceptable form capable of delivering bioavailable sulphur for agricultural use. Suitable forms include elemental sulphur such as but not limited to wettable sulphur, colloidal or micronized sulphur, sublimed or precipitated sulphur, as well as sulphur in the form of dispersible granules or suspension concentrates. Additionally, agrochemically acceptable derivatives or complexes of sulphur such as but not limited to calcium polysulphide (lime sulphur), other polysulphides, or sulphur-surfactant or sulphur-clay complexes may also be employed, provided that they are conventionally recognized for agricultural application. Further, sulphur may be incorporated into standard agrochemical formulations, with appropriate carriers or adjuvants. All such forms are intended to be encompassed within the term ‘sulphur’ as used herein, to the extent that they remain compatible with the claimed composition and exhibit acceptable agricultural utility.

[0089] The term ‘bioavailable sulphur’ as used herein refers to sulphur in any agrochemically acceptable form capable of releasing plant-usable sulphur under field conditions.

[0090] As used herein, the terms “bioactive quantity,” “efficacious amount,” or “effective weight percentage” refer to the amount of sulphur, or any other active ingredient of the present composition, which is sufficient to produce the intended agronomic response, including but not limited to enhancement of plant growth, improvement of nutrient assimilation, stimulation of physiological activity, or improvement of pest, disease, or stress tolerance. The bioactive or efficacious amount of sulphur corresponds to the quantity that provides available sulphur in a physiologically utilizable form, without causing phytotoxicity, formulation instability, or adverse interaction with the other active components.In the context of the present invention, sulphur may be employed in a concentration typically ranging from 0.001% to 10% by weight of the total composition, depending on the formulation type, mode of application, and compatibility with the accompanying plant growth regulators. The skilled person will recognize that the precise efficacious amount for sulphur, as well as for the other actives herein, may vary depending on the target crop, application method, and environmental conditions, and can be readily determined through routine optimization without undue experimentation.

[0091] As used herein, the expressions “in optimum quantities,” of each constituent, including sulphur and the accompanying plant growth regulators, corresponds to the concentration that provides maximum functional benefit under practical field or formulation conditions. Such amounts typically fall within a weight percentage range that can vary depending on the formulation type, but are readily determinable by a person skilled in the art through routine formulation trials and optimization without undue experimentation.

[0092] The term “plant health” or “health of a plant” refers to a condition of the plant wherein improved vigour, growth, and resilience to abiotic or biotic stresses are achieved, resulting in increased yield and / or improved quality of plant products.

[0093] The term “essential nutrient” refers to an element or compound that is required by plants which plays an essential role in their growth, development, metabolism, and overall physiological functions. The term is intended to encompass such elements / compounds, which are capable of correcting or preventing deficiencies and improving plant health and productivity.

[0094] Accordingly, in an especially preferred embodiment according to the invention, the health of a plant is increased both in the presence and absence of biotic or abiotic stress factors. An increase in plant vigor may for example result in an increased yield and / or tolerance to abiotic or biotic stress.

[0095] One indicator for the condition of the plant is the yield. “Yield” is to be understood as any plant product of economic value that is produced by the plant including but not limited to leaves, grains, fruits, vegetables, nuts, grains, seeds, wood (e.g., in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals), or any other part of plant, etc. The plant products may in addition be further utilized and / or processed after harvesting.According to embodiments of the present invention, the yield of the plants treated according to the method of the invention, is increased synergistically.

[0096] According to embodiments of the invention, the plant vigour of the treated plant is increased. In another preferred embodiment of the invention, the plant vigour of the plants treated according to the composition of the present invention is increased synergistically.

[0097] Another indicator for the condition of the plant is the “quality” of a plant and / or its products. According to embodiments of the invention, the quality of the treated plant is increased.

[0098] In preferred embodiment of the invention, the quality of the plants treated according to the method of the invention, is increased synergistically.

[0099] The term "Increased uptake of active ingredient" refers to the enhanced absorption or penetration of the active component of a substance into the target site of plant. This increased uptake can lead to greater effectiveness or efficacy of the product in achieving the desirous and intended results. Overall, increased uptake of the active ingredient results in improved performance of the product in achieving its intended purpose.

[0100] Another embodiment of the present invention, includes within its scope, all possible salts, esters and derivatives of the active ingredients of the composition.

[0101] It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification.

[0102] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.

[0103] It has been observed that the synergistic agrochemical composition of the present invention, not only exhibits a synergistic growth-regulating effect but also imparts effectiveness acrossall developmental stages of plants, including but not limited to germination, vegetative growth, flowering, fruiting, and maturation, etc.

[0104] In an embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0105] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0106] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) Bio-effective amount of Sulphur.

[0107] In another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0108] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0109] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0110] C) Bio-effective amount of sulphur; and

[0111] D) One or more agrochemically acceptable excipients.

[0112] In another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0113] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0114] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0115] C) Bio-effective amount of sulphur; and

[0116] D) At least one or more additives.

[0117] In one another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0118] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0119] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives;

[0120] C) Sulphur;

[0121] D) At least one or more additives selected from Urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and Epoxide soybean oil either alone or in combination thereof; and

[0122] E) One or more agrochemically acceptable excipients.In one another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0123] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0124] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; and

[0125] C) Sulphur, in the range of 0.001-10% by weight of the composition.

[0126] In one another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0127] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0128] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; and

[0129] C) Bio-effective amount of sulphur, in the range of 0.001-10% by weight of the composition; and

[0130] D) One or more agrochemically acceptable excipients.

[0131] In one another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0132] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0133] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; and

[0134] C) Bio-effective amount of sulphur, in the range of 0.001-10% by weight of the composition; and

[0135] D) At least one or more additives.

[0136] In one another embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0137] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0138] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;C) Bio-effective amount of sulphur, in the range of 0.001-10% by weight of the composition;

[0139] D) At least one or more additives selected from Urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and Epoxide soybean oil either alone or in combination thereof, in the range of 0.001-20% by weight of the composition; and

[0140] E) One or more agrochemically acceptable excipients.

[0141] In a specific embodiment, the present invention provides a stable synergistic agrochemical composition comprising:

[0142] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-25% by weight of the composition;

[0143] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-25% by weight of the composition; and

[0144] C) Sulphur, in the range of 0.001-10% by weight of the composition.

[0145] In yet another embodiment, the present invention provides a process for preparation of the stable synergistic agrochemical composition comprising:

[0146] A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;

[0147] B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) A bio-effective amount of Sulphur.

[0148] According to embodiments of the present invention, the stable synergistic agrochemical composition results in an increased yield of the treated plant.

[0149] According to embodiments of the present invention, the stable synergistic agrochemical composition comprising plant growth regulator, i.e., Triacontanol along with sulphur exhibit an improved plant growth regulation effect when applied in combination with a plant activator, i.e., Brassinolide in synergistically effective amounts.

[0150] In one of the embodiments, the stable synergistic agrochemical composition or formulation according to the present invention has a reduced or zero phytotoxicity with respect to various agricultural crops.In accordance with embodiments of the present invention, there is provided a stable synergistic agrochemical composition comprising active ingredients present in the weight % range (w / w% or w / v%) as given below:

[0151]

[0152] The composition of the present invention increases plant height, root and shoot development, number of flowers opened (e.g., in tomato), fruit set, and overall plant vigour, plant height, etc. The composition is effective in enhancing crop yield and quality, including size, weight, colour, and nutritional value, and act synergistically both in the presence and absence of biotic or abiotic stress factors.

[0153] In another embodiment of the present invention, the stable synergistic agrochemical composition of the present invention further comprises one or more agrochemically acceptable excipients including but not limited to the group comprising surfactant(s), dispersing agent(s), co-dispersant, anti-freezing agent(s), emulsifier(s)( (anionic and non-ionic), anti-foaming agent / defoamer(s), wetting agent(s), antimicrobial / anti-bacterial agent(s), anti-caking agent Thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s), pH-adjusting agent(s), anti-caking agent(s), adjuvant(s), filler(s) / suspension aid(s), colorant(s), carrier(s), buffering agent(s), polymer(s)rheology modifier(s), solvent(s), cosolvents) , biocide or a combination thereof. Additional components may also be included, e.g., protective colloids, carriers, adhesives, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid carrier / additive, which complies with usual formulation techniques.

[0154] Additional components may also be included, e.g., protective colloids, carriers, adhesives (liquid sticker / sticker- spreader), thixotropic agents, penetration agents, UV stabilisers, antioxidants, sequestering agents or chelating agents. More generally, the active materials can be combined with any solid or liquid carrier / additive, which complies with usual formulation techniques.An adjuvant used in the present invention is any material that is added to an agrochemical formulation to enhance or modify the performance of the formulation. Adjuvants used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxicity effects on any part of the plant.

[0155] Examples of surfactant(s) and / or dispersing agent and / or co-dispersant used herein include but not limited polymeric anionic dispersant, polyarylphenyl ether phosphate, tristyryl phenol ethoxylate, Polymethyl methacrylate -polyethylene glycol graft copolymer, acrylate copolymer, acrylic copolymer, polymeric dispersant, Calcium salt of alkyl benzene sulphonate, Non-ionic surfactant, polyalkoxylated butyl ether, acrylic graft copolymer, Sodium methyl oleoyl taurate , non-ionic proprietary surfactant, , Fatty Alcohol Ethoxylate, Sodium Polycarboxylate, Methyl naphthalene sulfonic acid, polymer with formaldehyde, sodium salt, ethoxylated tristryl phenol Sulphate, alkyl naphthalene formaldehyde condensate , naphthalene sulfonic acid, phenol sulfonic acid-formaldehyde polycondensation as sodium salt, sodium salt condensate with formaldehyde, ethoxylated oleyl cetyl alcohol, Sodium Alkyl Naphthalene Sulfonate Blend, styrenated phenol ethoxylate, polyalkelene glycol ether, Sodium Isopropyl Naphthalene Sulfonate, Dialkyl naphthalene sulphonate sodium salt, Naphthalene Sulphonic Acid, Formaldehyde, Acrylate graft Copolymer, Ethoxylated fatty glyceride , Ethoxylated Polyarylphenol Phosphate Ester, Lignosulfonic Acid, Sodium Salt, Sulfomethylated, ethoxylated fatty alcohol, methyl naphthalene sulfonic acid, polymer with formaldehyde sodium salt, Sodium Alkylnaphthalene Sulfonate Formaldehyde Condensate, Sodium polymethyl methacrylate, sodium polyacrylate, alkyl naphthalene sulfonate formaldehyde condensate or its sodium salt , Sodium Naphthalene Sulfonate formaldehyde condensate, sodium lignosulphonate, calcium lignosulphonate, Sodium Alkyl Naphthalene Sulfonate, Naphthalene Sulphonic Acid, Formaldehyde , alkylphenol polyoxyethylene ether, sodium salt of naphthalene sulfonate condensate, alkyl naphthalene sulfonate condensate blend, polymeric surfactant, sodium salt of alkyl naphthalene sulfonate, sodium salt of methyl naphthalene sulfonate, polycarboxylate, naphthalene sulfonic acid-branched and linear derivative sodium salts, fatty alcohol polyoxyethylene ether, alcohol polyglycol ether, non-aqueous dispersant -non-ionic polymeric surfactant, aqueous dispersant - polymeric amphoteric aqueous dispersant or mixtures thereof and present in the range of 0.1-20% by weight of the composition.

[0156] Examples of wetting agent used herein include but not limited to tristyrylphenol ethoxylate non-ionic emulsifier, polyalkoxylated butyl ether, mixture of non-ionic surfactants, Blend ofAnionic and Non-ionic, alkoxylated alcohol, block copolymer, alcohol polyglycol ether, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate, Calcium dodecylbenzene sulfonate Di-isopropyl Napthalene sulphonate, Benzene sulfonic acid, Hydroxy polymer with formaldehyde, Phenol and Urea sodium salt, Polyalkoxy ethers, alkyl phenol ethoxylates, tri-styrenated phenol ethoxylate, EO / PO block co-polymers, butyl polyalkylene oxide block copolymer, sodium lauryl sulphate, sodium sulphates or mixtures thereof and present in the range of 0.1-20% by weight of the total composition.

[0157] Examples of defoamer or antifoaming agent used herein include but not limited to silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane, trisiloxane ethoxylates, silicone oil, silicone compound, C10~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, tallow based fatty acids, polyalkyleneoxide polydimethylsiloxane emulsion and mixtures thereof and present in the range of 0.01-5.0 % by weight of the composition.

[0158] Examples of anti-freezing agent used herein include but not limited to glycol, propylene glycol, mono ethylene glycol, glycerine, diethylene glycol, sorbitol, urea, mannitol, polyethylene glycol or mixtures thereof and present in the range of 0.01-20% by weight of the composition.

[0159] Examples of fillers / binders / suspension aids used herein include but not limited to silicon dioxide, china-clay, kaolin, starch, bentonite, precipitated silica, fumed silica, lactose monohydrate, talcum powder, lactose, ammonium sulphate, water, sodium Sulphate, magnesium sulphate, sodium citrate, sodium chloride, potassium chloride, polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), methyl cellulose, and carboxymethyl cellulose, polyisocyanate, polyterpene resin and mixtures thereof and present in the range of 0.1-40 % by weight of the composition.

[0160] Examples of biocide or anti-microbial agent used herein include but not limited to 1, 2,-Benzisothiazolin -3-one, blends of 1,2,- benzisothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, blends of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, zinc l-hydroxy-2(lH)-pyridinethione, blends of zinc 1-hydroxy-2(lH)-pyridinethione and zinc oxide, and 2-n-octyl-4- isothiazolin-3-one or mixture thereof and present in the range of 0.01 to 15 % by weight of the composition.Examples of rheology modifier or thickeners or thickening agents used herein include but not limited to polysaccharides, carboxymethyl cellulose, bentonite clay, aluminium magnesium silicate, hydroxy propyl cellulose, xanthan gum, gum arabic, Silica powder, gaur gum, carbomer, chitosan, precipitated silica or mixtures thereof and present in the range of 0.01-5.0 % by weight of the composition.

[0161] Examples of emulsifier(s) used herein include but not limited to ionic or non-ionic emulsifier, or a blend or mixtures thereof and present in the range of 0.01-20 % by weight of the composition

[0162] Examples of non-ionic emulsifiers used herein include but not limited to araliphatic or aliphatic non-ionic oil emulsifier - ethoxylated sorbitol hexaester (derived from oleic acid), Ethoxylated mono-, di- and trialkylphenols, Ethoxylates of long-chain alcohols, Polyethylene oxide / polypropylene oxide homo- and copolymers, EO / PO block copolymers, Polymeric emulsifier (Nonionic polyalkylene oxide block copolymer), Ethoxylates of long-chain alkanols, Castor oil ethoxylate, Ethoxylated monoalkylphenol, Ethoxylates of cetyl alcohol and / or stearyl alcohol, Tristyrylphenol ethoxylate, Ethoxylated Tristyrylphenol or a mixture thereof, present in the range of 0.01-20.0 % by weight of the composition.

[0163] Examples of ionic emulsifiers used herein include but not limited to alkali metal and ammonium salts of alkyl sulfates, Calcium dodecylbenzene sulfonate, Hemisulfates of ethoxylated alkanols and ethoxylated alkylphenols, Alkylsulfonic acids, Bis(phenylsulfonic acid) ethers and their alkali metal or ammonium salts, Sodium lauryl sulfate, Sodium (cetyl / stearyl) sulfate, or a mixture thereof.

[0164] Examples of solvent(s) and co-solvent(s) used herein include but not limited to polar and nonpolar solvent from the group comprising heavy aromatic hydrocarbon, N methyl pyrollidone (NMP) , Solvent Naphtha (Solvent C-9), Di methyl sulfoxide (DMSO), Dimethylformamide (DMF), N- alcohol, 2-ethyl hexanol , alkyl amide, mineral oil, distilled water, aromatic solvents, water or mixtures thereof and present in the range of 1.0 to 40% by weight of the composition.

[0165] Examples of colouring pigment(s) / dye(s) used herein include but not limited to red dye FGR03, Permanent Rubin L5-B-01, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1 , pigment blue 80, pigment yellow 1 , pigment yellow 13, pigment red 112, pigmentred 48:2, pigment red 48: 1 , pigment red 57: 1 , pigment red 53:1 , pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, violet 23, acid red 51 , acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108 or mixtures thereof and present in the range of 0.01- 10.0 % by weight of the composition.

[0166] Examples of polymer(s) used herein include but not limited to polyethylene wax, polypropylene wax, paraffin wax, ethyl cellulose, cellusose acetate, polyvinyl acetate, polyvinyl butyral, acrylic polymers, polyurethane polymers, Sodium Polyacrylate, Calcium stearate, Stearic acid, isocyanate, Cross-linked carboxymethyl cellulose, Hydroxyethyl cellulose-acrylate graft copolymers, polyisocyanate used is toluene-2, 4-diisocyanate (TDI), diethylenetriamine (DETA), ethylene diamine (EDA), triethylenetetramine (TETA), and / or hexamethylene diamine (HMD A), diethanolglycine sodium salt, ethanoldiglycine disodium salt, hydroxymonocarboxylic acid compounds, hydroxydicarboxylic acid compounds, tetrasodium N,N-bis(carboxylatomethyl)-L-glutamate (GLDA), methylene diisocyanate (MDI), and / or low molecular weight polydiisocynates or mixtures thereof and present in the range of 0.01- 10.0 % by weight of the composition.

[0167] Examples of preservative(s) used herein include but not limited to Methyl paraben (Methyl p-hydroxybenzoate), Propyl paraben (Propyl p-hydroxybenzoate), Methylisothiazolinone (MIT), Chloromethylisothiazolinone (CMIT), Benzisothiazolinone (BIT), DMDM Hydantoin, Imidazolidinyl Urea, Phenol, Cresol, Chlorocresol, Benzoic acid, Sorbic acid, Salicylic acid, Thymol (natural preservative), Sodium benzoate, Potassium sorbate, Bronopol (2-bromo-2-nitropropane-l,3-diol), Propyl para-hydroxy -benzoate or mixture thereof and present in the range of 0.01- 15 % by weight of the composition.

[0168] Examples of stabilizer(s) used herein include but not limited to butylated copolymer , Butylated Hydroxy Toluene (BHT), Butylated Hydroxyanisole (BHA), Ascorbic Acid (Vitamin C), Tocopherol (Vitamin E), 2-(2H-Benzotriazol-2-yl)-4,6-di-tert-pentylphenol (a benzotriazole UV absorber), Hindered Amine Light Stabilizers (HALS), Oxanilide derivatives, Ethoxylated Alkylphenols (e.g., nonylphenol ethoxylates), Block copolymers, Polyvinyl alcohol (PVA), Acrylic or Butylated Copolymers, Citric acid / Sodium citrate, Phosphates (e.g., Sodium or Potassium phosphate), Acetates (e.g., Sodium acetate), EDTA (Ethylenediaminetetraacetic acid), Citric acid (dual role: pH and chelation), NTA (Nitrilo triacetic acid), Xanthan gum,Attapulgite clay, Bentonite, Hydroxypropyl methylcellulose (HPMC) or mixture thereof and present in the range of 0.01- 15 % by weight of the composition.

[0169] Examples of pH adjusting agent(s) / buffering agent(s) used herein include but not limited to Citric Acid, Citric Acid Monohydrate, Citric Acid Dihydrate, Sodium Citrate, Potassium Citrate, Tartaric Acid, Potassium Hydrogen Tartrate, Lactic Acid, Sodium Phosphate (Monobasic or Dibasic), Potassium Phosphate (Monobasic / Dibasic), Sodium Bicarbonate, Sodium Carbonate, Ammonium Acetate / Ammonium Hydroxide, Acetic Acid, Sodium Acetate, Borax (Sodium Borate) or mixture thereof and present in the range of 0.01- 15 % by weight of the composition.

[0170] Examples of Sequestering or chelating agent(s) used herein include but not limited to Ethylenediaminetetraacetic acid (EDTA) and its salts (disodium EDTA, tetrasodium EDTA), diethylenetriaminepentaacetic acid (DTPA) and nitrilotriacetic acid (NTA), Ethylenediamine-N,N'-disuccinic acid (EDDS), Phosphonic acid derivatives such as aminotris(methylene phosphonic acid) (ATMP) and diethylenetriaminepenta(methylene phosphonic acid) (DTPMP), citric acid, gluconic acid, and tartaric acid or mixture thereof and present in the range of 0.01- 5 % by weight of the composition.

[0171] In one embodiment, additives such as Siloxane Polyalkyleneoxide Copolymer or an organosilicone based powder adjuvant that can enhance the water absorption efficiency of hard-to-wet soils by increasing their water infiltration, drainage, water retention and hydrophilicity; tallow amine ethoxylate increases the biocompatibility in environmental applications and help in the interactions of active ingredients; ammonium sulphate help is resistant to leaching and helps the crop to utilize nitrogen efficiently; urea clathrate provides superior wetting and spray retention properties and serves as an excellent nitrogen source for crops and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition; and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition. The said additives are stable and hygroscopic resulting in an efficient uptake and functioning.

[0172] In one embodiment, additives such as Siloxane Polyalkyleneoxide Copolymer or organosilicone-based powder adjuvant, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof, present in the range of 0.001 - 20% by weight of the composition.In yet another embodiment of the present invention, the present stable synergistic agrochemical composition can be formulated as one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Controlled release (CR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Granular (GR), Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW).

[0173] In yet another embodiment of the present invention, the invention further provides the process for preparation of the said stable synergistic agrochemical composition, wherein the said composition can be one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Controlled release (CR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Granular (GR), Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW).

[0174] In yet another preferred embodiment of the present invention, the present stable synergistic agrochemical composition can be formulated as Granular (GR), Water dispersible granules (WG), Wettable powder (WP), Suspension Concentrate (SC), and Controlled -release (CR).In yet another most preferred embodiment of the present invention, the present stable synergistic agrochemical composition can be formulated as Granular (GR), Water dispersible granules (WG), and / or Controlled-release (CR).

[0175] In an embodiment of the present invention, a stable synergistic agrochemical composition further optionally comprises additives and / or adjuvants, for providing the following attributes to the composition of present invention:

[0176] a) enhanced stability,

[0177] b) enhanced permeability, and improved penetration capacity,

[0178] c) uniform spray-ability,

[0179] d) improved rain-fastness, and enriched biotic factors,

[0180] e) better flowability, and enhanced spreading or coverage effect,

[0181] f) effective storage stability,

[0182] g) longer residual effect, and improved uptake of active ingredients, and

[0183] h) safer to the environment.

[0184] According to embodiments of the present invention, the agrochemical composition of present invention can be applied to a plant / crop / soil by spraying, rubbing, atomizing, irrigating, evaporating, dusting, fogging, broadcasting, pouring, mist blowing, soil mixing, foaming, painting, spreading-on, drenching, dipping or drip irrigation. Etc.

[0185] In yet another embodiment of the present invention, the present stable synergistic agrochemical composition can be applied to various crops and plants including but not limited to Grape fruit, sweet cherry, Grapes, Brinjal, Paddy, Sugarcane (Planted crops), Cotton, Groundnut, Banana, Tomato, Potato, Cabbage, Tea, Mulberry, Rice, Wheat, Maize, Cauliflower, Apple, Oilseed rape, Sugar-beet, Chilli, Mango, Pineapple, etc.

[0186] The composition of present invention improved the crops agronomic factors such as including but not limited to height, number of flowers in crops / plants.

[0187] The composition of the present invention can be used to control and promotes pre-bloomelongation, berry size-enlargement, panicle emergence, enhancement of fruit size and weight, also improves the shape and development of prominent calyx lobes (typiness), increase lateral bud break and shoot growth (branching) & improving branch angle of nursery stock young trees, prevent shedding of flower squares & bolls, induce flowering and uniform growth, increase fruit size, etc.The stable synergistic agrochemical composition of the present invention can also comprise or may be applied together and / or sequentially with further active compounds. These further compounds can be selected from fertilizers or micronutrient donors or microorganisms or other preparations that influence plant growth, such as inoculants (e.g., a strain of nitrogen -fixing bacteria), and plant inducers.

[0188] The composition of present invention is highly safe to the user and to the environment. The composition offers the user a single homogenous application eliminating the need for tank mix. The composition also is cost-effective as it provides much greater simultaneous control and can be used in a variety of crops with a broader spectrum of protection.

[0189] The stable synergistic agrochemical composition of the present invention results in:

[0190] i. Enhanced photosynthesis, root and shoot growth, flowering, and fruit set;

[0191] ii. Improved uptake of phosphorus and other essential micronutrients, ensuring balanced nutrition;

[0192] iii. Increased crop yield and superior quality of plant products;

[0193] iv. Greater tolerance to abiotic stresses such as drought, salinity, and temperature fluctuations, as well as improved defense against biotic stresses;

[0194] v. Reduced application rates, lowering input costs and minimizing environmental residues;

[0195] vi. Stability and convenience as a single, ready-to-use liquid formulation with superior penetration, uniform spray coverage; and

[0196] vii. Non-phy to toxicity, environmental safety, and improved cost-benefit ratio.

[0197] The composition is capable of providing a single, homogenous application that ensures simultaneous plant growth regulation, nutrient supplementation, and disease protection, thereby reducing labor, application frequency, and overall agrochemical input.

[0198] The composition of present invention achieves high field efficacy at low application rates, reducing the overall plant growth regulator / nutrient load on the environment. The reduced dosage requirement translates into cost savings for farmers, while the extended residual and synergistic effects reduce the frequency of applications. By minimizing chemical residues and improving nutrient-use efficiency, the composition contributes to sustainable agriculture and environmental safety.Thus, before describing the present invention in detail, it is to be understood that this invention is not limited to particularly exemplified systems or process parameters that may of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.

[0199] In one embodiment, the composition according to the present invention acts synergistically to control pathogens and increases yield in various crops.

[0200] Representative examples of the present composition of this invention are provided below and are not to be construed as limiting in any manner:

[0201] EXAMPLES: GENERAL RECIPE OF COMPOSITION OF PRESENT INVENTION:

[0202]

[0203]

[0204]

[0205]

[0206] EXAMPLE A. Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % SC

[0207]

[0208] FORMULATION PROCESS

[0209] 1. DM water and mixture of surfactants (Acrylic graft copolymer, Ethoxylated Fatty Alcohol), Epoxide Soybean Oil, Sodium Isopropyl Naphthalene Sulfonate & Propylene Glycol (PG) were charged and further diluted in required D.M. Water.

[0210] 2. The required technicals i.e., Brassinolide Technical, Triacontan-l-ol Technical, Sulphur Technical, were then mixed to obtain homogeneous mass by high shearing.

[0211] 3. The above mixed mass was ground in bead Mill. Grinding was carried out until a mean particle size of below 5 microns was obtained.

[0212] 4. A 2% gum solution was prepared by taking 100 g DM water and 0.2 g of 1,2- Benzisothiazolin-3-one, and then under slow stirring, 2 g xanthan gum was added. 5. After the grinding, 2% water solution of xanthan gum was added under low stirring and mixed until a homogeneous mixture was obtained

[0213] 6. Finally, the quality parameters were checked.STABILITY DATA: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % SC

[0214]

[0215] EXAMPLE B: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % WP

[0216]

[0217] MANUFACTURING PROCESS:

[0218] 1. All required technicals, i.e., Brassinolide Technical, Triacontan-l-ol Technical, Sulphur Technical, and inert ingredients, were mixed and then ground in an air jet mill.

[0219] 2. The particle size was checked after grinding to ensure that it was less than 10 microns (D90).

[0220] 3. The ground powder was post-blended and mixed well.

[0221] STABILITY DATA: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % WP

[0222]

[0223]

[0224] Example C: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 WG

[0225]

[0226] MANUFACTURING PROCESS:

[0227] China clay was taken in the ribbon blender for mixing. To this, Silica ppt, Sodium Isopropyl Naphthalene Sulfonate, Sodium Lignosulphonate, Sodium Methyl Oleoyl Taurate, Sodium Polycarboxylate, Lactose monohydrate, Brassinolide Technical, Triacontan-l-ol Technical, Sulphur Technical were added and mixed well.

[0228] Mixing was continued until the material became completely homogenized. The premix was then passed through the Airjet mill at 6 to 7 kg / cm2of grinding pressure and a feeding rate of 100 to 150 kg / hr so as to obtain a particle size of D90 less than 10 microns. The ground material was collected in the post blender.Extrusion process

[0229] Into the post blender, the solution of water and Polydimethylsiloxane emulsion was added by means of spraying, and a dough was prepared. This dough was mixed well to obtain a well-hydrated mass for extrusion. The dough was then allowed to pass through the screw valve fitted with the post blender and subsequently passed through the basket extruder.

[0230] Drying Process

[0231] The wet extruded granules were passed through the VFBG (vibro fluid bed drier), which had been brought to a consistent temperature of 50 °C prior to drying. The granules were then checked for moisture content, which was required to be not more than 3%. Once the parameter was set, the process was continued.

[0232] The dried granules were passed through a vibro sieve machine to separate any lumps or dust, and the desired size granules were collected and packed.

[0233] STABILITY DATA: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 WG

[0234]

[0235] EXAMPLE D: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % GR

[0236]

[0237] MANUFACTURING PROCESS

[0238] 1. The required quantity of filler / carriers, dispersing agent, and Brassinolide Technical, Triacontan-l-ol Technical, Sulphur Technical were charged into a premixing blender and homogenized for 30 minutes.

[0239] 2. The pre-blended material was then ground through a jet mill / air classifier mill. The finely ground material was blended in a post blender until it became homogeneous (approximately 1.5 hours).

[0240] 3. The homogeneous material was analyzed, and after receiving approval from the QC department, the material was unloaded into 25 kg HDPE bags with LDPE liners inside.

[0241] 4. Subsequently, the required quantity of sand was charged into the granulator.

[0242] 5. DM water, sticking agent, and the remaining material were added and mixed until homogeneous.

[0243] 6. The finely ground material was then added to completely coat the sand and mixed well.

[0244] 7. The final product was sent for QC approval, and after approval, the material was packed in the required pack sizes.

[0245] STABILITY TEST: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % GR

[0246]

[0247] <

[0248]

[0249] EXAMPLE E: Brassinolide 0.01 % + Triacontanol 0.05 % + Sulphur 4 % CR

[0250]

[0251] MANUFACTURING PROCES

[0252] 1. Prepared mill base of Brassinolide, Tricontanol and Sulphur technical along with calcium ligno sulphonate, Sodium salt of methyl naphthalene formaldehyde condensate, l,2-benzisothiazolin-3 -one and distilled water.

[0253] 2. Separately prepared a solution mixture of alkyl phenol ethoxylate and ethoxylated tristyrlphenol phosphate blend and PE wax.

[0254] 3. In a coating pan bentonite granule was charged and the rotating pan was started. 4. On top of these bentonite granules the technical mill base was added and mixed well to uniformly adsorb the slurry.

[0255] 5. Using spray nozzle solution mixture of alkyl phenol ethoxylate and ethoxylated tristyrlphenol phosphate blend and PE wax were added.

[0256] 6. Added kaolin to dry the wet bentonite granules.

[0257] STABILITY TEST: Brassinolide 0.01 % + Triacontan-l-ol 0.05 % + Sulphur 4 % CR

[0258]

[0259] <

[0260]

[0261] EVALUATION OF SYNERGISTIC EFFECT OF STABLE SYNERGISTIC AGROCHEMICAL COMPOSITION OF THE PRESENT INVENTION

[0262] A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.

[0263] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0264] XY

[0265] E = (X + Y) - - 100

[0266] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0267] (XY+YZ+XZ) (XYZ)

[0268] E = (X + Y + Z) - - + - 100 10000

[0269] Where:

[0270] E represents expected percentage of PGR effect for the combination of the two or three active ingredients at defined doses (for example equal to x, y and z, respectively).

[0271] X is the percentage of PGR effect observed by the compound (Brassinolide) at a defined dose (equal to x).Y is the percentage of PGR effect observed by the compound (Triacontan-l-ol) at a defined dose (equal to y).

[0272] Z is the percentage of PGR effect observed by the compound (Sulphur) at a defined dose (equal to z).

[0273] When the percentage of PGR effect observed for the combination is greater than the expected percentage, there is a synergism effect.

[0274] Observed control (%)

[0275] Ratio = - Expected control (%)

[0276] Ratio of O / E > 1, synergism observed

[0277] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.

[0278] Experiment for Synergistic activity of stable synergistic agrochemical composition of present invention:

[0279] Example 1- Details of experiment on tomato crop:

[0280] A field experiment was conducted to study the synergistic effect of Brassinolide + Triacontan-l-ol + Sulphur on plant height and number of flowers (per plant) in tomato crop (Variety: Pusa Ruby) at Ganganagar, Rajasthan.

[0281] To evaluate the synergistic effect, tomato crop was sown in 6 m x 5 m plots maintaining 75 cm x 45 cm plant distance with three replications and in Randomized Block Design (RBD). The treatments were applied as foliar spray in the form of SC formulation @ 250 ml / ha. The treatment details are as under:

[0282]

[0283]

[0284] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two foliar sprays were applied, the first at 35 days after transplanting and the second at a 14-day interval after the first spray. Observations of the plant height and number of flowers in tomato were recorded at key intervals: before spraying, 14 days after 1stand 2ndapplications. The percent increase of plant height was recorded from three randomly selected branch per plant and 10 plants per plot. While the number of flowers per plant was counted during the peak flowering stage from randomly selected tagged plants in each treatment. The percent increase in plant height and flowers was calculated over control. Based on the percent reduction at 40 days after spray in Percent increase the Expected height / number of flowers was calculated and compared with Observed height / number of flowers. The results of the trial have been presented here under in Table 1A.

[0285] Table 1A: Synergistic effect of stable synergistic agrochemical composition of the present invention comprising “Brassinolide + Triacontan-l-ol+Sulphur” to increase “plant height” and “number of flowers” in tomato crop.

[0286]

[0287] It is clearly evident from the data shown in above Table 1A for percent change in different agronomic characters (plant height and number of flowers) in tomato crop that the stable synergistic agrochemical composition of the present invention comprising Brassinolide+ Triacontan-l-ol + Sulphur i.e., T1 to T4 are synergistic for agronomic characters (plant height and number of flowers) with > 1 Colby’s Ratio. The binary combinations (Brassinolide + Triacontan-l-ol, Brassinolide + Sulphur and Triacontan-l-ol + Sulphur) T5 to T16 are less efficacious and no synergistic effect is shown, as compared to stable synergistic agrochemical composition of present invention comprising Brassinolide + Triacontan-l-ol + Sulphur (T1 to T4). The above data is cumulative and demonstrates the synergistic effect of the stable synergistic agrochemical composition of the present invention.

[0288] Experiment for Bio-efficacy evaluation of stable synergistic agrochemical composition of the present invention:

[0289] For the ternary and binary combination based on Brassinolide + Triacontan-l-ol+ Sulphur evaluation under field conditions, the basic test composition as Brassinolide 0.01% + Triacontan-l-ol 0.05% + Sulphur 4% SC was manufactured. For ternary combination, Brassinolide 0.01% + Triacontan-l-ol 0.05% + Sulphur 4% SC was used and for binary combinations, Brassinolide 0.01% + Triacontan-l-ol 0.05% SC, Brassinolide 0.01%+ Sulphur 4% SC and Triacontan-l-ol 0.05% + Sulphur 4% SC were used. The solo formulations of Brassinolide 0.01% SC, Triacontan-l-ol 0.05% SC, Sulphur 4% SC were used for comparison. The other formulations namely Brassinolides 0.01% SP, Triacontan-l-ol 0.05% EC and Sulphur 80% WP available in the market were also used for comparison.

[0290] Example 2: Evaluation of the stable synergistic agrochemical composition of the present invention on tomato

[0291] A field experiment was conducted to study the synergistic effect of stable synergistic agrochemical composition of present invention comprising Brassinolide, Triacontan-l-ol, and Sulphur on plant height and number of flowers (per plant) in tomato crop (Variety: Pusa Ruby). To evaluate the synergistic effect, tomato crop was sown in 6 m x 5 m plots maintaining 75 cm x 45 cm plant distance with three replications and in Randomized Block Design (RBD).

[0292] Objectives:

[0293] 1. Bio-efficacy evaluation to increase plant height of tomato crop

[0294] 2. Bio-efficacy evaluation to increase number of flowers of tomato crop

[0295] 3. Bio-efficacy evaluation based on per cent increase plant height.4. Bio-efficacy evaluation based on percent increase number of flowers.

[0296] 5. Bio-efficacy evaluation based on crop yield.

[0297] 6. Phytotoxicity evaluation on tomato crop.

[0298] Treatment Details:

[0299]

[0300] Methodology:

[0301] The treatments T1 to T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied 35 days after transplanting in the experimental plots.

[0302] Observations of the plant height and number of flowers in tomato were recorded at key intervals: before spraying, 14 days after 1stand 2ndapplication. The percent increase of plant height was recorded from three randomly selected branch per plant and 10 plants per plot. While the number of flowers per plant was counted during the peak flowering stage fromrandomly selected tagged plants in each treatment. The percent increase in plant height was calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on tomato crop at 1, 3, 7, 10 and 14 days after 1stspray were also recorded. Results are presented in Table 2A, 2B, 2C and 2D.

[0303] Results:

[0304] Table 2A: Field bio-efficacy evaluation of the stable synergistic agrochemical composition of the present invention comprising Brassinolide 0.01% + Triacontan-l-ol 0.05%+ Sulphur 4% SC to increase plant height of tomato crop.

[0305]

[0306]

[0307] Table 2B: Field bio-efficacy evaluation of the stable synergistic agrochemical composition of the present invention comprising Brassinolide 0.01% + Triacontan-l-ol 0.05% + Sulphur 4% SC to increase number of flowers of tomato crop.

[0308]

[0309]

[0310] Table 2C: Field bio-efficacy evaluation of the stable synergistic agrochemical composition of the present invention comprising Brassinolide 0.01% + Triacontan-l-ol 0.05% + Sulphur 4% SC based on yield of tomato crop.

[0311]

[0312]

[0313] Table 2D: Phytotoxicity evaluation of the stable synergistic agrochemical composition of the present invention comprising Brassinolide 0.01% + Triacontan-l-ol 0.05% + Sulphur 4% SC on tomato crop.

[0314]

[0315]

[0316] From the example, it is clearly seen that the combination of the present invention is superior for increase the both plant height and number of flowers 14 days after 1stand 2ndspray (Table 2A and 2B) in tomato crop as compared to the other solo actives such as Brassinolide 0.01% SP, Triacontan-l-ol 0.05% EC and Sulphur 80% WP available in the market. The yield of tomato crop also improved in the stable synergistic composition of present invention comprising Brassinolide + Triacontan-l-ol + Sulphur as compared to binary and solo compositions (Table 2C).

[0317] The disclosed composition Brassinolide + Triacontan-l-ol + Sulphur @ 187.5ml, 250ml, 312.5 ml and 500 ml / ha further showed no phytotoxicity in the tomato crop even after 1, 3, 7, 10 and 14 days after spray (Table 2D).It is evident from the above data that the stable synergistic agrochemical composition of the present invention i.e., Brassinolide + Triacontan-l-ol + Sulphur resulted in good increase the both plant height and number of flowers in tomato with higher respective yields as compared to the reference standard products (binary compositions and solo products). Further, the composition of present invention is resulted synergistic.

[0318] In particular, it has now been found, surprisingly that, for example, the agrochemical activity of the composition of present invention, compared with the agrochemical activity of individual components, is synergistic. In fact, various advantageous properties associated with the compositions according to the invention, include but are not limited to: improved effect on plant height, plant growth, maintain leaf (colour & size), improve plant vigour / health, preventing discoloration of leaves of plants and crops, enhanced tenderness to plants and crops, preventing ageing of plants and crops and other advantages familiar to a person skilled in the art.

[0319] From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.

Claims

I / WE CLAIM1. A stable synergistic agrochemical composition comprising:A) Brassinolide or its agrochemically acceptable salts, esters and derivatives;B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives; and C) A bio-effective amount of Sulphur.

2. The stable synergistic pesticidal composition as claimed in claim 1, comprising:A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; andC) Bio-effective amount of Sulphur, in the range of 0.001-10% by weight of the composition.

3. A stable synergistic agrochemical composition comprising:A) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition; andC) Bio-effective amount of Sulphur, in the range of 0.001-10% by weight of the composition.D) At least one or more additive.

4. The stable synergistic pesticidal composition as claimed in claim 4, whereinA) Brassinolide or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;B) Triacontan-l-ol or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;C) Bio-effective amount of Sulphur, in the range of 0.001-10% by weight of the composition; andD) at least one or more additive selected from, Siloxane Polyalkyleneoxide copolymer, tallow amine ethoxylate, ammonium sulphate, and Epoxide soybeanoil either alone or in combination thereof, is in the range of 0.01-20% by weight of the composition.

5. The stable synergistic pesticidal composition as claimed in claims 1-4, which further comprises one or more agrochemically acceptable excipients.

6. The stable synergistic agrochemical composition as claimed in any one of claims 1 to 5, further comprises agrochemically acceptable excipients selected from the group consisting of surfactant(s), dispersing agent(s), co-dispersant, anti-freezing agent(s), emulsifier(s) (anionic and non-ionic), protective colloids(s), anti-foaming agent / defoamer(s), wetting agent(s), antimicrobial / anti-bacterial agent(s), anti-caking agent ,thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s), pH-adjusting agent(s), adjuvant(s), filler(s) / suspension aid(s), Anti-freezing agent(s), colorant(s), UV stabilizer, Antioxidant, liquid carrier(s), Sequestering / Chelating agents, buffering agent(s), polymer(s), rheology modifier(s), solvent(s), co-solvent(s) , biocide or a combination thereof.

7. The stable synergistic agrochemical composition as claimed in any one of claims 1 to 6 , wherein application of the composition increases plant height, number of flowers opened, root and shoot growth, leaf area index, canopy development, and overall plant vigor in crops including Grape fruit, sweet cherry, Grapes, Brinjal, Paddy, Sugarcane (Planted crops), Cotton, Groundnut, Banana, Tomato, Potato, Cabbage, Tea, Mulberry, Rice, Wheat, Maize, Cauliflower, Apple, Oilseed rape, Sugar-beet, Chilli, Mango, Pineapple, etc. .

8. The stable synergistic agrochemical composition as claimed in any one of claims 1 to 6, wherein the composition imparts tolerance to abiotic stresses to abiotic stresses including drought, salinity, and temperature fluctuations, and enhances plant defense against biotic stresses including fungal, bacterial, and viral pathogens.

9. A method for promoting plant growth, increasing crop yield and quality, and improving stress tolerance in a plant or crop, comprising the step of applying to the plant or soil asynergistic agrochemical composition as claimed in claims 1 - 7 in an effective amount sufficient to achieve the desired plant growth regulatory effect.

10. The stable synergistic agrochemical composition as claimed in claims 1- 7, wherein the composition provides residual physiological benefits to subsequent crops by improving soil nutrient availability and enhancing plant nutrient-use efficiency.

11. A process for preparing a stable synergistic agrochemical composition as claimed in claims 1-10.

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