Oral bacteria reducing agent for animal, oral bacteria reducing dentifrice for animal, oral bacteria reducing mouthwash for animal, oral bacteria reducing gum for animal, oral bacteria reducing gel for animal, and method for reducing oral bacteria in animal

Abstract

A major objective of the present invention is, among other things, to provide an oral bacteria reducing agent for an animal, said agent containing an active ingredient causing no problem even if absorbed into the body via the oral cavity of the animal over the long term, and being capable of reducing the bacterial counts of a plurality of causative bacteria of periodontal disease.　An oral bacteria reducing agent for an animal according to the present invention contains seaweed powder as an active ingredient, and is characterized in that the seaweed powder is a powder obtained by drying and pulverizing seaweed or a powder obtained by drying an extract of the seaweed, contains 0.01 mass% or more of iodine, and reduces the bacterial count of the oral bacteria by causing the oral bacteria reducing agent for an animal to be spread throughout the oral cavity of an animal.

Description

Oral fungal reducing agent for animals, oral fungal reducing toothpaste for animals, oral fungal reducing mouthwash for animals, oral fungal reducing gum for animals, oral fungal reducing gel for animals, and method for reducing oral fungi in animals

[0001] The present invention relates to an oral fungal reducing agent for animals, an oral fungal reducing toothpaste for animals, an oral fungal reducing mouthwash for animals, an oral fungal reducing gum for animals, an oral fungal reducing gel for animals, and a method for reducing oral fungi in animals.

[0002] Similar to humans, it is known that hundreds of types of bacteria inhabit the oral cavities of animals including dogs and cats. Examples of causative bacteria of periodontal disease in animals include Porphyromonas denticanis (P. denticanis bacterium), Porphyromonas salivosa (P. salivosa bacterium), Treponema denticola (T. denticola bacterium), Porphyromonas gulae (P. gulae bacterium), etc. Periodontal disease is a chronic disease in which the causative bacteria of periodontal disease grow in plaque attached to the periodontal pocket, which is the groove between the gum and the tooth, and discharge toxins, resulting in the destruction of the periodontal pocket and deepening of the pocket.

[0003] By the way, in recent years, toothpaste (tooth powder, liquid toothpaste, etc.), mouthwash (dental rinse, mouthwash, etc.), gum, gel, food, etc. that have the effect of removing the causative bacteria of periodontal disease in the oral cavity of animals and preventing periodontal disease are being sold. However, toothpaste and mouthwash often contain, for example, 1,3,3-trimethyl(1,8-cineole), thymol, methyl salicylate, cetylpyridinium chloride, etc., which are originally disinfectants and suppress the growth of the causative bacteria of periodontal disease, as bactericides, and in addition, dipotassium glycyrrhizinate, etc., which have a sedative effect on gum and oral inflammation. There are also some that contain antibiotics that have an effect of suppressing the growth of some bacteria. Furthermore, in order to efficiently remove dental plaque and tooth discoloration even for animals that dislike brushing their teeth, it is necessary to set a high concentration of the active ingredient. These components such as bactericides and antibiotics are not originally things that animals normally ingest daily, and they will be absorbed into the body through the oral cavity, and there was a problem that it was not desirable in the long term.

[0004] Furthermore, even if the components of a bactericidal agent (antibacterial agent) that kills the bacteria that cause periodontal disease are natural products, they are not usually consumed, or the antibacterial agent components are not water-soluble and therefore need to be dissolved in a solvent that is a non-natural product (artificially synthesized), and in addition, artificial sweeteners are sometimes used in combination with antibacterial agents as auxiliary agents (see Patent Document 1, etc.). Such water-insoluble / slightly water-insoluble natural bactericidal agents (antibacterial agents) also require the incorporation of non-natural solvents and additives for commercialization, which leads to direct or indirect absorption into the body, posing a problem that is undesirable in the long term.

[0005] Furthermore, Patent Document 2 discloses that a seaweed extract has specific antibacterial activity only against Porphyromonas gingivalis (P.g.), a bacterium known to cause periodontal disease in humans. However, since this antibacterial activity is expressed in terms of the minimum inhibitory concentration (MIC, ppm), there was a problem that the seaweed extract in Patent Document 2 inhibits growth but does not reduce the number of bacteria. In addition, although it has been shown that the antibacterial activity of chloroform-methanol extract is higher than that of water extract regarding Porphyromonas gingivalis (P.g.), only Fusobacterium nucleatum (F.n.) is disclosed as a bacterium that causes other periodontal disease, and moreover, the antibacterial activity of Fusobacterium nucleatum (F.n.) has not been confirmed. In other words, there was a problem that the seaweed extract in Patent Document 2 does not have antibacterial activity against multiple bacters that cause periodontal disease. Furthermore, since seaweed extracts require the removal of extraction residue during the extraction process, there was a challenge in that it was not possible to manufacture oral bacteria reducing agents that fully utilized the components of the seaweed before extraction.

[0006] Patent Document 3 shows that an oral composition for animals containing mastic resin and / or mastic essential oil as an active ingredient is effective in reducing Porphyromonas gulae in the oral cavity of animals. However, similar to Patent Document 1, there were problems such as the need to dissolve the antibacterial component in a solvent that is a non-natural product (artificially synthesized product) because it is not water-soluble.

[0007] Japanese Patent Publication No. 2020-620006, Japanese Patent Publication No. H9-48715, Japanese Patent Publication No. 2017-75098

[0008] The main objective of this invention is to provide an oral bacteria reducing agent for animals that contains an active ingredient that does not pose a problem even if absorbed into the body via the oral cavity of an animal over a long period of time, and that can reduce the number of one or more bacteria that cause periodontal disease.

[0009] In view of the above circumstances, the inventors conducted diligent studies and found an oral bacteria reducing agent for animals that contains an active ingredient that does not cause problems even if absorbed into the body via the oral cavity over a long period of time, and that can reduce the number of one or more bacteria that cause periodontal disease.

[0010] In other words, the present invention is an animal oral fungal reducing agent that contains seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine, and is characterized by reducing the number of oral fungi by spreading the animal oral fungal reducing agent throughout the oral cavity of the animal.

[0011] The oral bacteria of the animal may be selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae, with one or more species being chosen.

[0012] The oral bacteria of the animal may be selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral bacteria may be reduced simultaneously.

[0013] The aforementioned seaweed powder preferably has a pH of 5 to 8.

[0014] The present invention relates to an animal oral bacteria reducing toothpaste, characterized in that the above-mentioned animal oral bacteria reducing agent is incorporated as an active ingredient in toothpaste or liquid toothpaste.

[0015] The present invention is characterized by comprising the above-mentioned animal oral fungus reducing agent as an active ingredient in a mouthwash solution.

[0016] The present invention provides a gum for reducing oral bacteria in animals, characterized in that the above-mentioned oral bacteria-reducing agent for animals is incorporated into the gum as an active ingredient.

[0017] The present invention provides an animal oral fungus reducing gel, characterized in that the above-mentioned animal oral fungus reducing agent is incorporated into the gel as an active ingredient.

[0018] The present invention provides a method for reducing oral bacteria in animals, characterized by comprising the steps of: distributing the above-mentioned oral bacteria reducing agent for animals throughout the oral cavity of an animal; and repeating the step of distributing the agent throughout the oral cavity for a certain period of time.

[0019] The step of distributing the substance throughout the oral cavity may include distributing 0.8 g or more of the animal oral bacteria reducing agent throughout the oral cavity per day.

[0020] The present invention provides an oral microbiome reducing agent for animals that does not pose a problem even if the active ingredient is absorbed into the animal's body via the oral cavity over a long period of time, and that reduces the number of one or more bacteria that cause periodontal disease.

[0021] Embodiments of the present invention will be described in detail below.

[0022] (Animal Oral Bacteria Reducing Agent) The animal oral bacteria reducing agent of the present invention contains seaweed powder as an active ingredient. An animal oral bacteria reducing agent is an agent that has the effect of reducing the number of bacteria in the oral cavity of animals, in particular the number of fungi distributed in periodontal pockets. The reduction in the number of fungi is mainly thought to be due to the bactericidal effect, but the contribution of an antibacterial effect that prevents the proliferation of bacteria cannot be ruled out. Furthermore, "animal" as used herein refers to animals that need to have periodontal disease bacteria reduced, and examples include dogs, cats, monkeys, etc.

[0023] Veterinary oral bacteria reducing agents can reduce the total number of bacteria in the oral cavity of animals. In particular, they can reduce Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae, which are causative agents of periodontal disease in animals.

[0024] (Seaweed powder) Seaweed powder is a powder obtained by drying and grinding seaweed or by drying seaweed extract.

[0025] When drying and grinding seaweed to obtain a powder, theoretically, all components of the seaweed except water can be contained in the powder. Here, the drying temperature is preferably a temperature similar to that used for drying food (for example, around 60°C). The drying time should be adjusted to the point where the weight loss of the dried material is almost negligible if the remaining moisture content is nearly zero, or to a shorter drying time if some moisture is desired. The grinding time should be adjusted according to the particle size of the resulting powder.

[0026] When extracting seaweed and drying the extract to obtain a powder, the extraction residue is not contained in the powder. The composition of the resulting powder varies depending on the extraction solvent, extraction temperature, extraction time, etc. In particular, the components that are soluble and dissolve in the extract differ depending on the selection of the extraction solvent. Examples of extraction solvents include water, alcohol, organic solvents, and mixtures thereof. Furthermore, a drying and grinding step may be included before the extraction step to improve extraction efficiency.

[0027] The seaweed powder contains a certain amount or more of iodine as a component of the seaweed powder. The iodine content is 0.01 mass% or more, preferably 0.1 mass% or more, and more preferably 0.2 massss% or more. Examples of seaweeds that contain a certain amount or more of iodine include kelp, hijiki, and wakame. In order to ensure that the seaweed powder contains a certain amount or more of iodine, it is preferable to select seaweed with a high iodine content and to dry and grind the seaweed to obtain the powder so that the iodine is included in the powder. Even when drying an extract of seaweed to make a powder, it is acceptable as long as the extraction conditions allow for the specific extraction of iodine.

[0028] The pH of the seaweed powder is preferably between 5 and 8, and more preferably between 5.5 and 7. The pH of the seaweed powder is measured by dissolving a certain amount in water. Since it is for repeated oral use, if the pH is acidic (below 5), there is a higher possibility of damaging the oral mucosa or causing tooth erosion. Similarly, if the pH is alkaline (above 8), it is more likely to damage the oral mucosa.

[0029] Based on the pH range of the seaweed powder, it is thought that the iodine contained in the seaweed powder does not take the form of povidone-iodine (Isodine), which is known for its high bactericidal properties, i.e., a complex of polyvinylpyrrolidone and iodine. The pH of povidone-iodine is known to be around 1.5 to 3.5 in a 1% aqueous solution.

[0030] The particle size of the seaweed powder is set appropriately to ensure it can spread throughout the oral cavity, but a particle size of approximately 5 μm to 50 μm is a typical example. Since seaweed powder contains components that do not dissolve easily in water, a finer particle size is preferable for spreading throughout the periodontal pockets and other areas in the oral cavity.

[0031] (Oral bacteria) There are said to be several hundred types of oral bacteria in animals. Oral bacteria may include all oral bacteria. In this invention, "reducing the number of oral bacteria" means reducing the total number of oral bacteria. In addition, one or more species may be selected as oral bacteria from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae. Furthermore, all of the species from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola may be selected as oral bacteria. All of these are types of fungi that cause periodontal disease in animals, including dogs and cats.

[0032] There are various methods for diagnosing periodontal disease, but one example is evaluation by observing the condition of the oral cavity. An evaluation can be performed using the following criteria: a score of 0 indicates "no inflammation of the gums and healthy gums are observed. There is no plaque." A score of 1 indicates "mild periodontal disease" if the gingivitis is mildly red and swollen, and plaque covers less than one-third of the tooth surface. A score of 2 indicates "moderate periodontal disease" if the gums bleed when a probe is inserted and plaque covers less than two-thirds of the tooth surface. A score of 3 indicates "severe periodontal disease" if the gums bleed spontaneously and plaque covers more than two-thirds of the tooth surface.

[0033] Furthermore, there are various methods for examining bacteria in the oral cavity. One example is to collect plaque from the interdental spaces of designated locations using animal interdental paper points, suspend it in 1 mL of sterile phosphate buffer, freeze and store it at -15°C or below, and quantify the amount of DNA of each oral bacterium by real-time PCR.

[0034] The total number of oral bacteria in animals can be measured, for example, by collecting plaque from the interdental spaces of a designated location using an animal interdental paper point, suspending it in 1 mL of sterile phosphate buffer, storing it frozen at -15°C or below, and performing quantitative analysis using real-time PCR with the protein elongation factor Tu (tuf) gene.

[0035] (Application to the oral cavity) The animal oral fungus reducer is applied so as to spread throughout the oral cavity. For example, the animal oral fungus reducer suspended in sterile water for injection can be taken on a gloved index finger and applied by rubbing it directly between the animal's teeth. The powder of the animal oral fungus reducer may also be rubbed directly between the animal's teeth. Furthermore, teeth may be brushed with toothpaste or liquid toothpaste containing the animal oral fungus reducer as an active ingredient. The mouth may be rinsed with a mouthwash containing the animal oral fungus reducer as an active ingredient. The animal may be given gum containing the animal oral fungus reducer as an active ingredient to chew, or it may be incorporated into a gel and applied, or if it consists only of edible ingredients, it may be mixed into food.

[0036] (Other ingredients) While the oral bacteria reducing agent for animals uses seaweed powder as its active ingredient, the effectiveness in reducing oral bacteria in animals may vary due to individual differences, regional differences, seasonal differences, powder processing conditions, and even the conditions under which the oral bacteria reducing agent for animals is distributed within the oral cavity. Therefore, if necessary, it is possible to include auxiliary agents that have an oral bacteria reducing effect on their own in the seaweed powder.

[0037] The oral bacteria reducing agent for animals may further contain iodine powder and / or zinc powder as auxiliary agents. Unlike povidone-iodine, iodine powder has a pH of approximately 4.5 and exhibits a high bacterial reduction effect against periodontal disease bacteria. For this reason, even a small amount of iodine powder may function as an auxiliary agent, and the amount can be, for example, 0.01% by mass or more and less than 100% by mass relative to the mass of the seaweed powder, or it may be 0.01% by mass or more and less than 50% by mass. Iodine powder itself tends to reduce periodontal disease bacteria in a short time (for example, within 1 minute) and can be added as an auxiliary agent. Zinc powder also shows a bacterial reduction effect against periodontal disease bacteria, so it is thought to function as an auxiliary agent even with the addition of a small amount. The amount of zinc powder added may be, for example, 0.1% by mass or more and less than 40% by mass relative to the mass of the seaweed powder.

[0038] As an adjuvant, it may further contain one or more selected from the group consisting of phloroglucinol powder, black grape skin powder, and citric acid powder. Furthermore, it may contain laminaran powder, Ascophyllum nodosum powder, ascophyllin powder, β-carotene powder, nori micron powder, green tea, etc.

[0039] (Animal Oral Fungal Reduction Toothpaste) The animal oral fungal reduction toothpaste is a product obtained by formulating an animal oral fungal reducing agent as an active ingredient into toothpaste powder or liquid toothpaste.

[0040] (Animal Oral Fungal Reduction Mouthwash) The animal oral fungal reduction mouthwash is a product obtained by formulating an animal oral fungal reducing agent as an active ingredient into a mouthwash solution. By rinsing with the animal oral fungal reduction mouthwash, an appropriate amount of the animal oral fungal reducing agent is formulated so that the animal oral fungal reducing agent spreads throughout the oral cavity.

[0041] (Animal Oral Fungal Reduction Gum) The animal oral fungal reduction gum is a product obtained by formulating an animal oral fungal reducing agent as an active ingredient into gum. By chewing the animal oral fungal reduction gum, an appropriate amount of the animal oral fungal reducing agent is formulated so that the animal oral fungal reducing agent spreads throughout the oral cavity. In consideration of the fact that animals do not chew gum for a long time like humans but swallow it, it is preferable to adjust the blending amount of the active ingredient and other ingredients.

[0042] ​​​​​​The step of distributing the oral fungal reducing agent for animals in the oral cavity is exemplified by, for example, taking the oral fungal reducing agent for animals suspended in water for injection with a gloved index finger and applying it by directly brushing it between the teeth of the animal. The powder of the oral fungal reducing agent for animals may be directly brushed between the teeth of the animal. Furthermore, brushing the teeth with toothpaste or liquid toothpaste containing the oral fungal reducing agent for animals as an active ingredient may be performed. Rinsing the mouth with a mouthwash solution containing the oral fungal reducing agent for animals as an active ingredient may be performed. Chewing gum containing the oral fungal reducing agent for animals as an active ingredient may be done, or it may be formulated into a gel and applied, or if it consists only of edible ingredients, it may be mixed into food. The amount of the oral fungal reducing agent for animals used is appropriately adjusted according to the brushing time, the number of times of brushing, the rinsing time, the number of times of rinsing, the chewing time of the gum and the number of times thereof, the amount eaten as food, etc., but it is desirable to use 0.8 g or more per day to distribute it in the oral cavity of the animal, and it is more desirable to use 1.0 g or more.

[0045] The step of repeating the above-described step of distributing in the oral cavity of the animal for a certain period is performed, for example, for about 3 months to 1.5 years, or continuously thereafter, preferably daily, more preferably after each meal, by repeating brushing the teeth, rinsing the mouth, chewing gum, etc.

[0046] (Evaluation Test 1 - Evaluation of Reduction in Total Bacterial Count of Oral Fungi -) As the oral fungal reducing agent for animals, 2 mL of purified water was added to 0.8 g of kombu powder and mixed to prepare Sample A. Note that 0.8 g of kombu powder is used for one administration to one dog, so it was prepared in a lump for the required number of heads and the number of administrations.

[0047] As the animals to be evaluated, six dogs (beagles) aged 6 months or older were prepared. The breakdown was three dogs (2 males and 1 female) in the administration group to which Sample A was administered and three dogs (2 males and 1 female) in the control group (the group to which Sample A was not administered). All the dogs were clinical target animals, and their general condition was observed for 7 days before the evaluation test to confirm that they were healthy. During the acclimation period, dogs without any abnormalities were selected and assigned to the administration group and the control group by the stratified randomization method based on their body weights immediately before the start of the evaluation test.

[0048] In a closed-type barn, each animal was housed in a cage measuring W70.5 × D118.5 × H88 cm. A feeder and water dispenser were placed inside the cage. 300g of DS-A (Oriental Yeast Co., Ltd.) was given once a day as feed. Tap water was also provided as free drinking water. During the evaluation test, the room temperature was maintained within the range of 18-29°C. The lighting schedule consisted of 12 hours of light and 12 hours of dark.

[0049] The treatment group received sample A once daily for three months. The suspended sample A was applied by taking a small amount on a gloved index finger and rubbing it directly onto the upper gums and teeth of the dogs. The control group received no treatment.

[0050] During the 3-month administration period, all dogs in both the treatment and control groups were clinically observed once daily for general conditions such as energy levels, appetite, and fecal characteristics. The results of the clinical observations are shown in Table 1.

[0051]

[0052] As shown in Table 1, no clinical observational abnormalities were observed in either the treatment group or the control group.

[0053] During the 3-month administration period, the oral condition of all dogs in both the treatment and control groups was observed. Gum condition was evaluated according to the scores in Table 2. However, strictly speaking, gum condition varies depending on the position of the teeth, etc., and even with the same overall score, there may be cases where the scores are high / low and cases where they are not very varied. Also, there are four levels of scoring, and there are conditions such as the boundary area between score 0 and 1, and the boundary area between score 1 and 2. Therefore, the area of ​​plaque can also be used as a reference indicator.

[0054]

[0055] During the 3-month administration period, the condition of the oral cavity and surrounding gums (edema, bleeding, discoloration, redness, etc.) was checked after each administration. The results are shown in Table 3.

[0056]

[0057] Table 3 shows that in the control group, scores of 1-2 remained consistent from the start to the end of the evaluation study. In contrast, in the treatment group, dogs that showed a score of 1 at the start of the evaluation study dropped to a score of 0 within two weeks, and dogs that showed a score of 2 at the start of the evaluation study maintained a score of 2, but a decrease in plaque area was observed.

[0058] To examine the number of oral bacteria, plaque was collected from the interdental space between the upper left premolar and incisor at 0, 0.5, 1, 2, and 3 months from the start of the evaluation test using an animal interdental paper point (Absorbent Paper Point, TIANJIN GAPADENT), suspended in 1 mL of sterile phosphate buffer, and stored frozen at -15°C or below.

[0059] The above plaque suspension was quantitatively measured using real-time PCR with the protein elongation factor Tu (tuf) gene to confirm the total number of oral bacteria. Table 4 shows the total number of bacteria as the amount of DNA per 1 mL of extracted DNA solution (copies / mL).

[0060]

[0061] Table 3 shows that in the control group, although there were fluctuations in the values ​​from the start to the end of the evaluation test, the value remained at 10. 8 The levels were approximately copies / mL. In contrast, the treatment group showed the same levels as the control group at the start of the evaluation study, 10. 8 The count was initially copies / mL, but gradually decreased, reaching 10 copies / mL one to three months after the start of the evaluation test. 6 The result was copies / mL, which was two orders of magnitude lower than the control group. From the measurement of the total number of oral bacteria, it was clear that the total number of bacteria in the treatment group was significantly reduced compared to the control group.

[0062] (Evaluation Test 2 - Evaluation of Reduction of Porphyromonas denticanis -) The plaque suspension obtained in Evaluation Test 1 was quantitatively measured by real-time PCR to determine the number of Porphyromonas denticanis bacteria. Table 5 shows the number of Porphyromonas denticanis bacteria as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​below the detection limit (<4) were treated as 0.

[0063]

[0064] Table 5 shows that at the start of the evaluation study, Porphyromonas denticanis was detected in all animals in both the treatment group and the control group. In the control group, although the levels fluctuated during the evaluation study, no significant decrease was observed. In contrast, in the treatment group, from 0.5 months after the start of the evaluation study, Porphyromonas denticanis was below the detection limit (not detected) in all animals.

[0065] (Evaluation Test 3 - Evaluation of Reduction of Porphyromonas salivosa (P. salivosa bacteria) -) The plaque suspension obtained in Evaluation Test 1 above was quantitatively measured by real-time PCR to determine the number of Porphyromonas salivosa (P. salivosa bacteria). Table 6 shows the number of Porphyromonas salivosa (P. salivosa bacteria) as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​<4, which indicate a value below the detection limit, were treated as 0.

[0066]

[0067] Table 6 shows that at the start of the evaluation study, Porphyromonas salivosa was detected in all animals in both the treatment group and the control group. In the control group, although the levels fluctuated during the evaluation study, no significant decrease was observed. In contrast, in the treatment group, from two months after the start of the evaluation study, Porphyromonas salivosa was below the detection limit (not detected) in all animals.

[0068] (Evaluation Test 4 - Evaluation of the reduction of Treponema denticola (T. denticola bacteria) -) The plaque suspension obtained in Evaluation Test 1 above was quantitatively measured by real-time PCR to determine the number of Treponema denticola (T. denticola bacteria). Table 7 shows the number of Treponema denticola (T. denticola bacteria) as DNA amount per 1 mL of extracted DNA solution (copies / mL). Note that in the calculation of the average value, <10 3 The calculation was performed using 1000 as the value.

[0069]

[0070] Table 7 shows that in the control group, the period from the start of the evaluation test to the end of the evaluation test was 10 4 copies / mL~10 5 The levels were approximately copies / mL. In contrast, in the treatment group, from two months after the start of the evaluation study, all animals showed levels of Treponema denticola (T. denticola) below the detection limit (not detected).

[0071] (Evaluation Test 5 - Evaluation of Reduction of Porphyromonas gulae -) The plaque suspension obtained in Evaluation Test 1 above was quantitatively measured by real-time PCR to determine the number of Porphyromonas gulae bacteria. Table 8 shows the number of Porphyromonas gulae bacteria as DNA amount (ng / μL) per 1 μL of extracted DNA solution. In the calculation of the average value, values ​​below the detection limit (<4) were treated as 0.

[0072]

[0073] Table 8 shows that the number of Porphyromonas gulae bacteria in the treatment group decreased to about 1 / 2 to 1 / 10 of that in the control group one month after the start of the evaluation study.

[0074] The present invention may comprise the following items: [Item 1] An oral fungal reducing agent for animals, comprising seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine, and reduces the number of oral fungi by spreading the oral fungal reducing agent for animals throughout the oral cavity of the animal. [Item 2] The oral fungal reducing agent for animals according to Item 1, wherein the oral fungi of the animal are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae. [Item 3] The oral bacteria reducing agent for animals according to Item 1, wherein all of the oral bacteria of animals are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral bacteria is reduced simultaneously. [Item 4] The oral bacteria reducing agent for animals according to Item 1, wherein the seaweed powder has a pH of 5 or more and 8 or less. [Item 5] The oral bacteria reducing toothpaste for animals, wherein the oral bacteria reducing agent for animals according to Item 1 is incorporated as an active ingredient in toothpaste or liquid toothpaste. [Item 6] The oral bacteria reducing mouthwash for animals, wherein the oral bacteria reducing agent for animals according to Item 1 is incorporated as an active ingredient in mouthwash. [Item 7] A gum for reducing oral bacteria in animals, wherein the oral bacteria reducing agent for animals described in Item 1 above is incorporated into the gum as an active ingredient. [Item 8] A gel for reducing oral bacteria in animals, wherein the oral bacteria reducing agent for animals described in Item 1 above is incorporated into the gel as an active ingredient. [Item 9] A method for reducing oral bacteria in animals, comprising the steps of: distributing the oral bacteria reducing agent for animals described in Item 1 above throughout the oral cavity of an animal; and repeating the distributing step throughout the oral cavity for a certain period of time. [Item 10] The method for reducing oral bacteria in animals according to Item 9 above, wherein the distributing step throughout the oral cavity includes distributing 0.8 g or more of the oral bacteria reducing agent for animals throughout the oral cavity per day.

Claims

1. An oral fungal agent for animals containing seaweed powder as an active ingredient, wherein the seaweed powder is a powder obtained by drying and grinding seaweed or a powder obtained by drying an extract of seaweed, and contains 0.01 mass% or more of iodine, and reduces the number of oral fungi by spreading the oral fungal agent for animals throughout the oral cavity of the animal.

2. The oral fungal reducing agent for animals according to claim 1, wherein the oral fungi of the animal are selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, Treponema denticola, and Porphyromonas gulae.

3. The oral fungal agent for animals according to claim 1, wherein the oral fungi of animals are all selected from the group consisting of Porphyromonas denticanis, Porphyromonas salivosa, and Treponema denticola, and the number of the selected oral fungi is simultaneously reduced.

4. The animal oral fungus reducing agent according to claim 1, wherein the seaweed powder has a pH of 5 or more and 8 or less.

5. A toothpaste for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in claim 1 as an active ingredient in toothpaste or liquid toothpaste.

6. A mouthwash for reducing oral bacteria in animals, comprising the animal oral bacteria reducing agent described in claim 1 as an active ingredient in a mouthwash solution.

7. A gum for reducing oral bacteria in animals, wherein the oral bacteria reducing agent for animals described in claim 1 is incorporated into the gum as an active ingredient.

8. An animal oral fungus reducing gel, comprising the animal oral fungus reducing agent described in claim 1 as an active ingredient in the gel.

9. A method for reducing oral bacteria in animals, comprising the steps of: distributing the animal oral bacteria reducing agent described in claim 1 throughout the oral cavity of an animal; and repeating the step of distributing the agent throughout the oral cavity for a certain period of time.

10. The method for reducing oral bacteria in animals according to claim 9, wherein the step of distributing the agent throughout the oral cavity includes distributing 0.8 g or more of the animal oral bacteria reducing agent throughout the oral cavity per day.

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