Scrub composition comprising plant callus

Abstract

The present invention provides a scrub composition for exfoliation comprising plant callus, which maintains foam for a long time due to a natural surfactant component and rapidly and effectively removes dead skin cells due to the characteristic coarse formulation thereof.

Description

Scrub composition containing plant callus The present invention relates to a scrub composition for exfoliation, and more specifically, to a scrub composition comprising plant callus. Plant callus refers to an undifferentiated, irregular mass of cells, also known as plant stem cells, which is the tissue undergoing vigorous division around a wound when a plant body is injured. Plants are largely composed of meristematic tissue, which undergoes cell division, and non-meristematic tissue. When cells from the initial meristematic tissue are placed in a nutrient medium and grown, callus is formed, followed by the development of adventitious embryos that differentiate into plant bodies. Callus can be induced from various plant parts, such as stems, leaves, roots, or embryos. Callus culture is widely used in micropropagation—a technique that enables rapid plant reproduction in a controlled environment—making it efficient for the mass production of valuable plant species, including rare or endangered species. Furthermore, due to its vigorous division, callus contains high concentrations of bioactive substances effective for anti-inflammatory, antioxidant, anti-wrinkle, and skin whitening effects compared to ordinary plant tissues. In particular, it contains various types of phytochemicals effective for human skin, distinct from differentiated plant bodies, and is utilized as a core material for cosmeceuticals and bio-cosmetics. When plant callus is applied to the skin, it exhibits excellent skin cell proliferation ability and promotes collagen synthesis, contributing to wrinkle improvement and increased elasticity. Furthermore, it has anti-aging efficacy due to its antioxidant ability to eliminate free radicals, and depending on the plant species, it may also exhibit whitening and soothing effects. In this regard, Korean Registered Patent No. 1919113 discloses a composition for strengthening the skin barrier containing lotus callus extract. However, the aforementioned prior art relates to a composition that strengthens a damaged skin barrier by promoting the synthesis of proteins constituting the tight junctions of the keratinocyte layer, and is therefore unsuitable for use as an exfoliating scrub composition. The present invention aims to solve various problems, including the problems mentioned above, by providing an exfoliating scrub composition comprising plant callus that maintains foam for a long time with natural surfactant ingredients and rapidly and effectively removes dead skin cells due to its unique rough formulation. According to one aspect of the present invention, a scrub composition for exfoliation comprising plant callus as an abrasive is provided. According to another aspect of the present invention, a step of disinfecting plant tissue containing saponin through ultrasonic washing; A step of inducing a callus by culturing the above-mentioned disinfected plant tissue; A step of obtaining the above callus and producing dried callus powder through drying and grinding processes; A method for preparing an exfoliating scrub composition is provided, comprising the step of mixing the above callus powder with an excipient for formulating a cleansing cosmetic. The exfoliating scrub composition of the present invention, comprising plant callus as an abrasive as described above, contains a large amount of natural surfactant components and effectively removes dead skin cells without excessive physical irritation by producing small, long-lasting bubbles, thereby serving as an exfoliating scrub material capable of replacing the microplastic issues associated with conventional scrub products. Furthermore, mass production is possible by artificially culturing the plant callus under specific conditions. Of course, the scope of the present invention is not limited by these effects. FIG. 1 is a schematic diagram showing the manufacturing process of soapwort seed-derived callus manufactured according to one embodiment of the invention and the results of analyzing the effects of the said callus. Figure 2 is a graph showing the results of detecting saponarin, a natural surfactant component, from the soapwort seed-derived callus of the present invention using HPLC. Figure 3 is a graph showing the results of analyzing the cell viability of the soapwort seed-derived callus extract of the present invention using mouse macrophages. Figure 4 is a graph showing the results of analyzing the nitric oxide (NO) inhibitory effect of the soapwort seed-derived callus extract of the present invention. The effect on the generation of nitric oxide (NO), an indicator of inflammation, in inflammation-induced macrophages was analyzed using the Griess assay. Figure 5a is a graph showing the results of analyzing the effect of the soapwort seed-derived callus extract of the present invention on inhibiting the expression of iNOS, an inflammation mediating protein. Figure 5b is a graph showing the results of analyzing the inhibitory effect of the callus extract derived from soapwort seeds of the present invention on the expression of COX2, an inflammation mediator protein. Figure 5c is a gel image showing the results of a Western blot analysis of the effects on the expression levels of iNOS and COX-2, which are indicator proteins in the inflammation-inducing pathway. Figure 6 shows a foaming power test of the soapwort seed-derived callus extract of the present invention, where A represents the state before the foaming power test and B represents the state after the foaming power test. The order of the substances in A and B is the same. Figure 7 is a graph showing the results of a skin exfoliation test of a scrub formulation containing a callus extract derived from soapwort seeds according to the present invention. Figure 8 is a photograph showing the removal effect of cosmetics marked on the forearm of a subject using distilled water and 1% sea sand as the control group and six types of experimental substances. Figure 9 is a photograph of a subject's forearm marked in a circle using an ink pad (top) for an ink removal experiment (bottom). Figure 10 is a photograph showing the effect of removing ink marks marked on the forearm of a subject using distilled water and 1% sea sand as the control group and four types of experimental substances. Definition of Terms: The term "soapwort (Saponaria officinalis)" used in this document refers to a perennial plant belonging to the Caryophyllaceae family of the Caryophyllales order of dicotyledonous plants. The genus name Saponaria is derived from the Latin words sapo, meaning "soap," and -aria, meaning "related." The abundant flowers have five petals and are pink or white in color. Widely known as a natural cleanser, soapwort produces lather when its leaves or stems are rubbed, and due to its cleansing properties, it was used in the Middle East to wash hands or hair. As used in this document, the term "saponins" refers to a general term for substances that are glycosides of steroids, steroid alkaloids, or triterpenes, and which dissolve in water to exhibit soap-like foaming action. They exist in various plants and are also found in the bodies of some echinoderms (starfish, sea cucumbers). Saponins are known to be components that boost the human immune system; ginsenosides and eleutherosides, which are types of saponins found in ginseng, are particularly well-known, and it is also known that green tea contains various types of saponins. Detailed description of the invention: According to one aspect of the present invention, a scrub composition for exfoliation comprising plant callus as an abrasive is provided. In the scrub composition above, the callus may be in the form of a wet paste or a dry powder, and it is more preferable that it be in the form of a dry powder. In the scrub composition above, the plant may be a plant containing saponin, and the plant containing saponin may be selected from the group consisting of soapwort, kudzu, soybean, garlic, onion, ginseng, balloon flower, Acanthopanax senticosus, Rhus verniciflua, Iris pseudacorus, tea, Codonopsis pilosula, Yucca, Quillaja saponaria, Aesculus hippocastanum, Trifolium pratense, and Dioscorea villosa. The scrub composition may additionally include at least one additional component. The additional component may be selected from the group consisting of excipients, additives, and flavoring agents. In this case, the excipient may be water, oil, wax, emulsifier, dispersant, or solubilizer, and the additive may be a humectant, thickener, preservative, antioxidant, colorant, pH regulator, or deodorant, and the emulsifier refers to an additional surfactant that serves to ensure good mixing of the oily component and the water component within the composition. The scrub composition may not include the additional surfactant, and even if the additional surfactant is included, the additional surfactant component may be included in a lower amount than is typically included due to the natural saponin components contained in the plant callus containing saponin, so the scrub composition according to one embodiment of the present invention is more eco-friendly compared to conventional scrub compositions. The additional surfactant may be a saponin or a synthetic surfactant, and the additional surfactant may be included in an amount of 0.5 to 20 weight percent based on the total weight. The synthetic surfactant may be a neutral surfactant, an anionic surfactant, or a cationic surfactant. In the scrub composition above, the plant callus may be derived from the seeds, branches, roots, leaves, stems, or flowers of a plant. According to another aspect of the present invention, a step of disinfecting plant tissue containing saponin through ultrasonic washing; A step of inducing a callus by culturing the above-mentioned disinfected plant tissue; A step of obtaining the above callus and producing dried callus powder through a drying and grinding process; and A method for preparing an exfoliating scrub composition is provided, comprising the step of mixing the above callus powder with an excipient for formulating a cleansing cosmetic. In the above manufacturing method, the surfactant may be a natural surfactant and / or a synthetic surfactant, and the natural surfactant may be saponin, phospholipid, lecithin, cocobetaine, xanthan gum, beeswax, wax, borax, olive liquid, sulfosuccinate, yucca schidigera, alkyl polyglucoside, lauric acid, or apple wash, and the synthetic surfactant may be a nonionic surfactant, anionic surfactant, or amphoteric surfactant. The above nonionic surfactant may be a polyethylene glycol derivative or a polyalcohol derivative, and the polyethylene glycol derivative may be a polyoxyethylene sorbitol fatty acid ester, a polyoxyethylene fatty acid ester, a polyoxyethylene polyoxypropylene alkyl ester, or polyoxyethylene hydrogenated castor oil, and the above polyalcohol derivative may be glycerin, pentaerythritol, sorbitol, glycerin fatty acid ether, sorbitol fatty acid ester, polyglycerin fatty acid ester, propylene glycol fatty acid ester, or sucrose. The above anionic surfactant may be a carboxylate, a sulfate, a sulfonate, or a phosphate, the carboxylate may be sodium cocoamphoacetate or soap, the sulfate may be sodium lauryl sulfate (SLS), sodium dodecyl sulfate (SDS), ammonium lauryl sulfate (ALS), sodium laureth sulfate (SLES), ammonium laureth sulfate (ALES), olefin sulfate, TEA-lauryl sulfate, or TEA-laureth sulfate, the sulfonate may be sodium xylene sulfonic acid, alkylbenzene sulfonic acid, alkylnaphthalene sulfonic acid, IGEPON T, AEROSOL OT, or sulfonated paraffin salt, and the phosphate may be a higher alcohol phosphate. The above-mentioned amphoteric surfactant may be gelatin, glycinate, imidazoline derivative, betaine, cocoamidopropyl betaine, lauryl dimethyl betaine or imidazoline derivative, or phosphatide. In addition to the surfactant mentioned above, the surfactant added to the exfoliating scrub composition according to one embodiment of the present invention may include polyglutamic acid, dimethiconol, dimethicone copolyol, silicone glycol copolyol, silicone glycol copolymer, lauryl methicone copolyol, cyclomethicone dimethicone copolyol, monostearic acid glycerin, monooleic acid sorbitan, sesquioleic acid sorbitan, monostearic acid, polyoxyethylene sorbitan monooleate, polyoxyethylene (20) sorbitan monooleic acid, and PEG-40 stearate. The exfoliating scrub composition of the present invention can be formulated into formulations such as an exfoliating scrub agent, a cleansing foam, a cleansing cream, or a cleansing soap. In this case, depending on the formulation, the soapwort callus of the present invention may be included in an amount of 0.5 to 10 weight% based on the total weight. The exfoliating scrub composition of the present invention may additionally include excipients or additives in addition to the surfactant, wherein the excipient may be an emulsifier, water, oil, a dispersant, or a solubilizer, and the additive may be a moisturizer, a thickener, a preservative such as a preservative, an antioxidant, a pigment, an acidity regulator, or a deodorizer. The above moisturizer is extracted from plants and may include glycerin, 1,3-butylene glycol, dipropylene glycol, methylpropanediol, propylene glycol, Hinoki cypress extract, Houttuynia cordata extract, or propolis extract; additionally, polyols including glycerol, propylene glycol, and acrylate copolymers, and polyacrylic acid polymers may be further used. The above moisturizer may be contained in an amount of 2 to 30 weight%, preferably 5 to 28 weight%, and more preferably 10 to 25 weight% based on the total weight of the scrub composition. If the above moisturizer is less than 2 weight%, the skin moisturizing power decreases. If it exceeds 30 weight%, the amount of the above surfactant decreases relatively, resulting in insufficient foam formation and reduced moisturizing power. Among the above additives, the thickener may include one or more selected from the group consisting of acrylate copolymer, carbomer, acrylate / C10-30 alkyl acrylate crosspolymer, dimethicone / vinyl dimethicone crosspolymer, dimethicone crosspolymer, vinyl dimethicone, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, and combinations thereof. Among the above additives, the preservative may be any one or more mixtures selected from the group consisting of alkyl esters of para-hydroxybenzoic acid, hydantoin derivatives, propionates, and various quaternary ammonium compounds, phenoxyethanol, methylparaben (methyl parahydroxybenzoate), propylparaben (propyl parahydroxybenzoate), imidazolidinyl urea, sodium dehydroacetate, and benzyl alcohol. Among the excipients above, the oil may be a natural oil, and the natural oil may be selected from jojoba oil, sunflower oil, hydrogenated palm oil, avocado oil, olive oil, almond oil, macardum nut oil, meadowfoam oil, palm oil, coconut oil, castor oil, daisy oil, apricot kernel oil, evening primrose oil, camellia oil, tea seed oil, almond oil, peanut oil, shea butter, aloe butter, etc., hydrocarbons such as squalane, liquid paraffin, etc., and synthetic oils such as octyldodecanol, trioctanoin, isocetyl palmitate, isocetyl myristate, cetyloctanoate, neopentyl glycol, dicaprilate, diisopropyl sebacate, diisostearyl adipate, diisopropyl magadiate, trimethylolpropane trioctanoate, trimethylolpropane trilithearate, isopropyl myristate, and dimethicone. It is possible to use a mixture of one or more types, taking into account emulsification ability and economic feasibility. The stratum corneum acts as a barrier on the outermost layer of the skin to prevent the penetration of harmful foreign substances, while also protecting the skin's oil and moisture balance. Consequently, excessive removal weakens the skin and, in severe cases, can worsen into dry eczema. While an appropriate level of keratin protects the skin, excessive keratinization dulls the skin tone and hinders the absorption of moisture and nutrients. The stratum corneum (horney layer), located on the outermost part of the epidermis, undergoes a process where old keratinocytes shed over time and new ones take over its function; this repetitive series of changes is referred to as "epidermal cell differentiation" or "keratinization." However, external factors such as stress and environmental conditions, or internal factors such as aging, can cause keratinocytes in the stratum corneum to fail to shed normally, altering the shedding cycle or resulting in uneven shedding. When keratinocytes do not shed properly, the skin appears rough and dull, and the skin tone becomes uneven. In addition, it can clog pores and cause skin conditions such as acne. Meanwhile, plant callus culture can produce physiologically active compounds such as antioxidants, phytochemicals, vitamins, and growth factors beneficial to the skin, and said compounds can provide skincare benefits such as removing oxidative stress, promoting collagen synthesis, and strengthening skin moisture. However, in skincare formulations, utilizing plant callus as an exfoliating scrub composition is still an unexplored field. Accordingly, the inventors induced a callus derived from the seeds of *Saponaria officinalis* containing natural surfactant components and developed an exfoliating scrub composition utilizing the exfoliating scrub function of the callus, that is, its function as an abrasive (Fig. 1). The callus has a lumpy shape and, when dried, possesses the characteristics of a scrub due to its uniquely rough formulation. The inventors confirmed that when the callus derived from the *Saponaria officinalis* seeds was added to sterile water at a concentration of 1%, small, long-lasting bubbles were generated, and that skin exfoliation was effectively reduced when the product was applied to the skin of 20 to 30 subjects and rubbed. The scrub composition containing the plant callus of the present invention can reduce the content of chemically synthesized surfactants compared to existing cosmetics due to the natural surfactant (Saponarin) contained in the callus. Furthermore, it was confirmed that Saponarin contained in the scrub composition according to one embodiment of the present invention has a high anti-inflammatory effect. Therefore, when used in cosmetics containing both a scrubbing effect due to the unique tissue formulation of callus and a surfactant component, it can be utilized as a novel scrub composition material that has never existed before, having the effect of an eco-friendly scrub formulation that can replace microplastics and the foaming effect of a natural surfactant. Although soapwort was used as a representative example in the present invention, other plants rich in natural saponins other than soapwort, such as kudzu, soybeans, garlic, onions, ginseng, balloon flower, Acanthopanax senticosus, Rhus verniciflua, Iris pseudacorus, tea, Codonopsis pilosula, Yucca, Quillaja saponaria, Aesculus hippocastanum, Trifolium pratense, and Dioscorea villosa, may be selected from the group consisting of these. The present invention will be described in more detail below through examples. However, the present invention is not limited to the examples disclosed below but can be implemented in various different forms. The following examples are provided to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention. Example 1: Preparation of callus and extract 1-1: Preparation of Callus Induction Medium The inventors prepared an MS (Murashige and Skoog) callus induction medium for the production of calluses derived from Saponaria officinalis. Specifically, 3% (30 g / L) sucrose, 4.66 g of MS medium, and 0.8% (8 g) of plant agar (all products from Duchefa) were weighed and placed in a 1L glass bottle, and then 1L of water was added, after which the pH was adjusted to 5.6–5.8 using a 1N NaOH or 1N HCl solution. Subsequently, 2,4-D was added to the pH-adjusted medium at a concentration of 1 mg / L, sterilized in an autoclave at 120°C and 1.2 bar for 15 minutes, dispensed into Petri dishes in 25 ml portions, and allowed to solidify at room temperature. 1-2: Establishing Disinfection Conditions for Soapwort Seeds To establish sterile in vitro germination conditions for soapwort seeds, they were disinfected by ultrasonic washing in 70% ethyl alcohol for 2 minutes and 30 seconds and in a 12% sodium hypochlorite solution for 25 minutes. After removing the seed coats, the remaining disinfectants were washed off with sterile water. Subsequently, normal callus germination was confirmed while minimizing contamination of the disinfected seeds. 1-3: Establishment of Callus Induction Conditions To establish callus induction conditions using the disinfected soapwort seeds, the moisture from the soapwort seeds was removed, and they were placed on the callus induction medium prepared in Example 1-1 above. They were then cultured in an incubator at 28°C under dark conditions for 2 to 3 weeks to confirm that a callus derived from the soapwort seeds was induced. 1-4: Preparation of Scrub Agent The calluses derived from soapwort seeds, which were induced for 2 to 3 weeks as described above, were collected in one place and dried in a 60°C drying oven to completely remove moisture, and then ground in a mortar to obtain granular dried soapwort calluses in powder form. 1-5: Preparation of Callus Extract To prepare the extract of the above-mentioned soapwort seed-derived callus, the callus was washed five times in sterile triple-washed water, freeze-dried for 7 days, and then crushed. 50% ethyl alcohol was added, and the mixture was extracted at room temperature for 72 hours. Subsequently, ethanol was removed using an evaporator, and the mixture was freeze-dried to obtain a powder. The soapwort seed-derived callus extract powder was dissolved in DMSO at a concentration of 100 μg / mL, filtered, and then used. 1-6: Preparation of Quillaza and Balloon Flower Callus and Extracts The inventors selected Quillaja saponaria and Platycodon grandiflorus as other plants known to contain saponins other than soapwort, induced calluses from them, and obtained callus extracts therefrom. Specifically, the inventors induced callus and prepared callus extracts using the same method as in Examples 1-3 and 1-5, except that Quillaza was cultured in MS medium containing 1 mg / L of the hormone 2.4-D (2.4-dichlorophenoxyacetic acid), and Platycodon grandiflorus was cultured in MS medium containing 1 mg / L of the hormone kinetin and 2 mg / L of 2.4-D to induce callus. Example 2: High-Performance Liquid Chromatography (HPLC) Analysis The inventors performed HPLC analysis of the callus extract derived from soapwort seeds obtained from Example 1 above. Specifically, a 50% ethanol extract of the callus was used as the sample for HPLC analysis, and an Agilent 1260 was used for the HPLC. An Agilent Eclipse XDB-C18 column (4.6 × 250 mm, 5 μm) was used, and the reaction was carried out for 25 minutes with a gradient using two solvents, A (dH2O) and B (Acetonitrile), as the mobile phases at a flow rate of 1 ml / min. As a result, it was confirmed that the callus extract derived from soapwort seeds of the present invention contained the highest amount of saponarin, a component with excellent anti-inflammatory effects (Fig. 2). Example 3: Nitric Oxide (NO) Inhibitory Effect of Soapwort Callus Extract The inventors evaluated the toxicity of a callus extract derived from soapwort seeds prepared according to one embodiment of the present invention using mouse macrophages. Specifically, RAW264.7 mouse macrophages were inoculated into a 96-well plate at a density of 50,000 cells / ml, and after 24 hours, the medium was replaced with serum-free medium. After 24 hours, the cells were pretreated with the callus extract derived from soapwort seeds of the present invention at different concentrations (1, 5, 10, 15, 20, 25, and 30 μg / ml) for 24 hours. Subsequently, concentrations (5, 10, and 20 μg / ml) that did not affect cell viability were determined using the ez-cytox kit (Fig. 3). Then, the macrophages were inoculated into a 6-well plate at a density of 50,000 cells / ml, and after 24 hours, the medium was replaced with serum-free medium. After 24 hours, the above callus extract was treated with lipopolysaccarides (LPS), an inflammation-inducing substance, at a concentration of 1 μg / ml. Subsequently, the absorbance of nitric oxide (NO) released from the cells was measured at 540 nm using an ELISA reader via the Griess reaction method, and the experiment was repeated three times under the same experimental conditions. As a result, a significant NO inhibitory effect of the callus extract derived from soapwort seeds of the present invention was confirmed (Fig. 4). Example 4: Inhibitory effect on the expression of inflammatory mediator proteins The inventors analyzed the inhibitory effect of the callus extract derived from the seeds of the present invention on the expression of inflammatory mediator proteins iNOS and COX2. Specifically, mouse macrophages RAW264.7 were seeded into a 6-well plate at a density of 50,000 cells / ml, and after 24 hours, the medium was replaced with serum-free medium. After 24 hours, the cells were treated with the callus extract derived from the seeds at different concentrations (5, 10, and 20 µg / ml) and pretreated with lipopolysaccarides (LPS), an inflammatory inducing substance, at a concentration of 1 µg / ml for 24 hours. Subsequently, the inhibitory effect of the callus extract derived from the seeds on the expression of inflammatory mediator proteins iNOS and COX2 was analyzed by Western blot. As a result, a significant inhibitory effect of the callus extract derived from the seeds of the present invention on the expression of iNOS and COX2 was confirmed (Figs. 5a to 5c). Example 5: Foaming power test Surfactant components have the characteristic of easily generating foam in an aqueous solution by lowering the surface tension of water, so measuring foaming power serves as an indicator to visually confirm surfactant ability. Accordingly, the inventors performed foaming power tests on the dried callus powder derived from soapwort seeds prepared in Examples 1-4 of the present invention, the dried callus powder from Quillaza and balloon flower root prepared in Examples 1-65. Specifically, for the foaming power test, 10 ml of solution was added to a conical tube with a diameter of 20 mm, and then 1% by weight of each callus powder (soapwort, Quillaza, balloon flower root, and tobacco) was added (Fig. 6a). The tube was shaken 10 times under the same conditions at room temperature, and the height of the bubbles was measured in mm immediately after they were generated. Synthetic surfactant tween20 (Polysorbate 20) was used as a positive control at a concentration of 0.01%. As a result, it was confirmed that the soapwort callus exhibited high foaming power (2.4 cm) and retention power compared to the control group and other experimental groups (Fig. 6b). Meanwhile, although the Quillaza callus and balloon flower callus were somewhat lower than those of soapwort, they also showed excellent foaming power compared to tobacco callus that did not contain saponin. Example 6: Skin Irritation Evaluation The inventors conducted a skin irritation test to utilize the soapwort callus scrub formulation prepared from Examples 1-4 as a cosmetic. Specifically, the primary skin irritation test was commissioned to the Olive Clinical Research Center located in Yongin-si, Gyeonggi-do, and was performed on 30 subjects (3 men and 27 women) from May 30, 2023 to June 1, 2023. The subjects did not exhibit any specific skin symptoms during the skin irritation test and had no history of diseases or medication use that could affect the test. The test method involved drying soapwort callus, crushing it into a scrub foam, and diluting it in sterile water to a concentration of 1% (20 µl). The back of a subject, free from discoloration or skin damage, was wiped with 70% ethanol, dried, and then patched and left on for 24 hours. After removing the patch, the test area was marked with a medical skin marker pen and removed after 1 hour. Then, a specialist visually observed the area to evaluate the degree of skin irritation according to the evaluation criteria of the International Contact Dermatitis Group (ICDRG) (see Tables 1 and 2). After 24 hours, the degree of irritation at the patch site was visually observed again, and the average skin reactivity was calculated according to the skin reactivity calculation formula, Equation 1 below. (Formula 1) Mean score calculation formula Mean score = A= Score i B= Score j i = Number of study subjects 1 hour after patch removal j = Number of study subjects 24 hours after patch removal As a result, the average score for skin irritation was 0.28, confirming that the scrub formulation of the above-mentioned soapwort callus does not cause skin irritation when applied to the human body (see Table 3). The judgment criteria of the International Contact Dermatitis Research Group are summarized in Table 1 below, the results of the primary skin irritation reading are summarized in Table 2 below, and the results of the skin irritation evaluation are summarized in Table 3. International Contact Dermatitis Study Assessment Criteria Symbol Score Assessment Criteria - 0 Negative ± 0.5 Suspicious or mild reaction and erythema + 1 Erythema + Induration ++ 2 Erythema + Induration + Vesicles +++ 3 Erythema + Induration + Blisters Primary Skin Irritation Reading Criteria Average Score Non-irritating (Grade 1) 0.00 ~ 0.75 Low Irritation (Grade 2) 0.76 ~ 1.50 Mild Irritation (Grade 3) 1.51 ~ 2.50 Moderate Irritation (Grade 4) 2.51 ~ 4.00 High Irritation (Grade 5) 4.01 ~ Skin Irritation Evaluation Result Name Presence / Absence of Irritation Average Score Judgment Soapwort Seed-Derived Callus 0.28 Non-irritating Example 7: Exfoliation Evaluation 7-1: Skin Exfoliation Improvement The inventors conducted a skin exfoliation efficacy test of the soapwort callus scrub formulations prepared from Examples 1-4 above. Similarly, the exfoliation evaluation test was commissioned to the Olive Clinical Research Center located in Yongin-si, Gyeonggi-do, and was performed on 21 subjects (1 male and 20 females). The test method involved preparing a soapwort callus formulation by diluting dried and ground soapwort callus into a scrub foam in sterile water to a concentration of 1%, and then rubbing it on the subjects' forearms to assess the degree of exfoliation using a Visioscan, a device that measures human skin topography, dryness, and sebum production. ®The degree of desquamation improvement before and after application was assessed using the VC 20plus and the desquamation index (%) values ​​of the test site using Formula 2 below. In addition, to determine the significance of the instrument measurements or analysis values ​​before and after use, the statistical analysis program SPSS Statistics 27 Standard was used for verification. Statistical significance was confirmed when the p-value was < 0.05 within the 95% confidence interval. (Equation 2) As a result, it was confirmed that the product has a very excellent effect on exfoliation, showing a statistically significant difference with a change rate of 44.07% immediately after use compared to before use of the soapwort callus preparation (p<0.001) (Table 4 and Fig. 7). In addition, a very positive effect on skin exfoliation improvement was confirmed through a subjective survey evaluation conducted on subjects immediately after use of the soapwort callus preparation (see Table 5). The results of the exfoliation improvement evaluation are summarized in Table 4 below, and the survey evaluation results are summarized in Table 5 below. Exfoliation Improvement Evaluation Results Within-group Comparison Peeling Index (%) Before Use After Use Mean ±SD 5 3.92 ±12.50 30.55 ±9.39 Significance Probability -<0.001 *** Change rate (%) - 44.07 * :p<0.05 ** :p<0.01 *** :p<0.001 by pairedttest Survey Evaluation Results Classification Number of People Immediately After Use Agreement Response Rate (%) * 1. My skin feels smoother. 2. My skin feels softer. 3. The amount of dead skin cells seems to have decreased. 4. It feels gentle and non-irritating to the skin. 5. I would be willing to purchase it if it is released at a reasonable price. 6. I would be willing to recommend it to acquaintances or friends. *Positive response rate: Calculated by summing 4-point (generally satisfied), 5-point (satisfied), and 6-point (very satisfied) responses. 7-2: Cosmetics Experiment To objectively confirm the exfoliating effect of the scrub composition of the present invention, the inventors [conducted] Experiment Example 4 of the prior Korean Registered Patent No. 1913795, cleansing effect experiment

[0077] and Experimental Example 2 of Korean Published Patent No. 2021-0045003, comparative experiment on cleansing effect

[0066] Cosmetic experiments were conducted to indirectly evaluate the exfoliation effect by referring to [the source]. A total of six experimental substances were prepared: ① tobacco extract without saponin (10 mg / ml), ② soapwort extract containing saponin (10 mg / ml), ③ soapwort callus scrub (1%), ④ a mixture of soapwort extract (10 mg / ml) and soapwort callus scrub (1%), ⑤ a mixture of Quillaza callus 1% and Quillaza extract 10 mg / ml, and ⑥ a mixture of balloon flower callus 1% and balloon flower extract 10 mg / ml. Distilled water and 1.00% sea sand, which is used in scrub formulations, were used as the control group. Subsequently, a square shape was marked darkly on the subject's forearm using a cosmetic pencil liner. Then, small amounts of the six experimental substances were sequentially applied to the marked area, and the degree to which the cosmetics were removed was tested through physical friction using a finger. As a result, as shown in Fig. 8, it was confirmed that the mixture of ⑥ soapwort extract (10 mg / ml) and soapwort callus scrub (1%) exhibited a superior removal effect compared to ③ soapwort extract (10 mg / ml) and ④ soapwort callus scrub (1%), and the mixture of Quillaza extract + Quillaza callus 1.0% and the mixture of balloon flower extract + balloon flower callus 1.0% also showed a very excellent cosmetic cleansing effect (Fig. 8). This suggests that plants containing saponins other than soapwort can also be used in the scrub composition of the present invention. 7-3: Ink experiment The inventors also applied a thick circular mark to the forearm of a subject using an ink pad, and analyzed the cleansing power of four experimental substances, including the soapwort callus prepared in Example 1, by assessing the degree of removal of the ink imprinted on the skin. Distilled water and 1% sea sand were used as control groups. A total of six experimental substances, including two control groups, were sequentially applied in small amounts to the corresponding marked areas, and the degree to which the ink was removed was tested through physical friction using a finger (Fig. 9). As a result, consistent with the cosmetic experiment results, the mixture of ⑥ soapwort extract (10 mg / ml) and soapwort callus scrub (1%) showed the highest removal effect (Fig. 10). Preparation Example 1: Preparation of a scrub agent The scrub agent containing the powdered soapwort callus of the present invention can be prepared using the components of Table 7 below. Scrub Ingredients Ingredient Content Scrub Soap Glue Callus 1.6 Surfactant: Sodium Laureth Sulfate 3.0 Moisturizer: Glycerin 20.0 Thickening Agent: Acrylate Copolymer 2.4 Preservative: Phenoxyethanol 0.50 Other Additives: Castor Seed Oil 0.5 Solvent: Purified Water Residue Preparation Example 2: Preparation of cleansing foam The cleansing foam containing the powdered soapwort callus of the present invention can be manufactured using the components of Table 8 below. Cleansing Foam Ingredients Ingredient Content Scrub: Soapwort Callus 1.6 Surfactant: Sodium Laureth Sulfate 3.0 Moisturizer: Glycerin 20.0 Thickening Agent: Acrylate Copolymer 2.40 Preservative: Phenoxyethanol 0.5 Other Additives: Allantoin 0.5, Potassium Cocoate 5.0 Solvent: Purified Water Residue Preparation Example 3: Preparation of cleansing cream The cleansing cream containing the powdered soapwort callus of the present invention can be prepared using the components of Table 9 below. Cleansing Cream Ingredients Ingredient Content Scrub: Soapwort Callus 1.6 Surfactant: Sodium Laureth Sulfate 3.0 Moisturizer: Glycerin 20.0 Thickening Agent: Acrylate Copolymer 2.40 Preservative: Phenoxyethanol 0.50 Other Additives: Sunflower Seed Oil 0.5 Stearyl Alcohol 1.0 Shea Butter 1.0 Sorbitol 2.0 Solvent: Purified Water Residue Preparation Example 4: Preparation of cleansing soap The cleansing soap containing the powdered soapwort callus of the present invention can be manufactured using the components of Table 10 below. Cleansing Soap Ingredients Ingredient Content Scrub: Soapwort Callus 1.6 Surfactant: Sodium Laureth Sulfate 3.00 Moisturizer: Glycerin 20.00 Thickening Agent: Acrylate Copolymer 2.40 Preservative: Phenoxyethanol 0.50 Other Additives: Hyaluronic Acid 2.50 Natural Oil Fragrance 0.50 Rosemary 1.0 Peppermint 1.0 Sorbitol 5.0 In conclusion, the exfoliating scrub composition containing plant callus of the present invention does not irritate human skin and inhibits the production of nitric oxide (NO) in a concentration-dependent manner at a concentration that does not affect cell viability in mouse macrophages (RAW264.7), and significantly reduces the protein expression levels of iNOS and COX, thereby being useful as a natural surfactant, natural scrub, and pharmaceutical raw material for anti-inflammatory purposes. Although the present invention has been described with reference to the embodiments described above, this is merely illustrative, and those skilled in the art will understand that various modifications and equivalent alternative embodiments are possible therefrom. Accordingly, the true technical scope of protection of the present invention should be determined by the technical spirit of the appended claims.

Claims

1. An exfoliating scrub composition comprising plant callus as an abrasive.

2. In Paragraph 1, The above-mentioned callus is a composition in the form of a wet paste or a dry powder.

3. In Paragraph 1, A composition in which the above-mentioned plant is a plant containing saponin.

4. In Paragraph 3, A composition comprising a plant containing the above saponin selected from the group consisting of soapwort, kudzu, garlic, onion, ginseng, balloon flower, sweet flag, tea, codonopsis, yucca, Quillaja saponaria, horse chestnut (Aesculus hippocastanum), red clover (Trifolium pratense), and wild yam (Dioscorea villosa).

5. In Paragraph 1, A composition comprising at least one additional cosmetic ingredient.

6. In Paragraph 5, A composition in which the above additional component is one or more selected from the group consisting of excipients, additives, and flavoring agents.

7. In Paragraph 6, The above excipient comprises at least one additional surfactant, forming a composition.

8. In Paragraph 7, A composition in which the above additional surfactant is a saponin or a synthetic surfactant.

9. In Paragraph 7, A composition comprising 0.5 to 20 weight percent of the above additional surfactant based on the total weight.

10. In Paragraph 9, A composition in which the above synthetic surfactant is a neutral surfactant, an anionic surfactant, or a cationic surfactant.

11. In Paragraph 1, The above plant callus is a composition derived from the seeds, branches, roots, leaves, stems, or flowers of a plant.

12. A step of disinfecting plant tissues containing saponins through ultrasonic washing; A step of inducing a callus by culturing the above-mentioned disinfected plant tissue; A step of obtaining the above callus and producing dried callus powder through a drying and grinding process; and A method for preparing an exfoliating scrub composition, comprising the step of mixing the above callus powder with an excipient for formulating a cleansing cosmetic.

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