Nucleic acid encoding the signal peptide for expressing CRM197 protein, nucleic acid construct, expression vector, recombinant microorganism, and method for producing a CRM197 protein.
Optimized signal sequences and nucleotide sequences enable efficient expression and secretion of CRM197 protein into the periplasm of E. coli, addressing production inefficiencies and toxicity issues, resulting in high-purity and high-yield CRM197 production.
Patent Information
- Authority / Receiving Office
- BR · BR
- Patent Type
- Applications
- Current Assignee / Owner
- GENOFOCUS CO LTD
- Filing Date
- 2019-10-02
- Publication Date
- 2026-07-07
AI Technical Summary
Existing methods for producing CRM197 protein in E. coli result in low efficiency and toxicity to the host cells due to improper folding and proteolytic cleavage, limiting mass production for use in vaccines.
Optimized signal sequences and nucleotide sequences are designed to facilitate efficient expression and secretion of CRM197 protein into the periplasm of E. coli, utilizing specific amino acid sequences (SEQ ID NO: 13 to SEQ ID NO: 21) and nucleotide sequences (SEQ ID NO: 4 to SEQ ID NO: 12) to enhance translation and minimize toxicity.
The CRM197 protein is expressed in a soluble form and secreted into the periplasm efficiently, achieving high purity and productivity, with properties similar to native CRM197, overcoming the limitations of cytoplasmic production.
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