A fluorescent color calibration card for endoscopes and its preparation method and application

Abstract

The present invention belongs to the technical field of fluorescent materials, and relates to a fluorescent color calibration card for endoscopes, and its preparation method and application. The fluorescent color calibration card for endoscopes includes a color-developing sheet, and the color-developing sheet includes a fluorescent dye, an adsorption material, and a substrate. The preparation method of the color-developing sheet includes the following steps: uniformly mixing the adsorption material with water or an aqueous solution containing an adhesive, then pouring it on a substrate, smoothing it, drying it, and cutting it to a suitable size to form an adsorption sheet; immersing the adsorption sheet in a fluorescent dye solution, taking it out and drying it to obtain a color-developing sheet. The fluorescent color calibration card for endoscopes provided by the present invention can be used for parameter calibration of fluorescent endoscope equipment, judgment of the operating status of fluorescent endoscope equipment, and auxiliary adjustment of the operating status of fluorescent endoscope equipment under different fluorescent modes, which makes up for the defect that the current fluorescent endoscope lacks a matching color calibration card.

Description

Technical Field

[0001] The invention belongs to the technical field of fluorescent materials and relates to a fluorescent color calibration card for an endoscope and a preparation method and application thereof. Background Art

[0002] Fluorescence endoscope is a new type of diagnostic equipment that uses laser intrinsic fluorescence spectroscopy technology to enter the cavity for diagnosis through various endoscopes. It automatically identifies and diagnoses based on the intrinsic fluorescence spectral characteristics of human tissue, and can immediately indicate whether the tested tissue is normal tissue. It can also distinguish benign and malignant lesions of the tested tissue, thereby improving the diagnosis rate of early cancer and dysplasia.

[0003] Currently, pre-use calibration of fluorescence endoscope equipment parameters relies on operator experience. Common methods for parameter setting and instrument status determination primarily involve machine self-tests, in vitro fluorescent dye smear testing, and adjustments during use. These methods not only suffer from inaccurate parameter settings, waste of fluorescent dye, and difficulty in standardized operation, but also pose risks for clinical examinations and intraoperative navigation when the instrument is not used under appropriate conditions, and there is also the possibility of damage to the device probe. Therefore, there is an urgent need to develop a fluorescence calibration card that can be used with a variety of fluorescence equipment, including fluorescence endoscopes, and scientific research equipment.

[0004] Color calibration charts have become essential accessories for a variety of other imaging and photographic equipment, including white balance calibration charts and standard color palettes for cameras and mobile phones, color calibration charts for printing and scanning equipment, and sensitive gradient calibration charts for military near-infrared or thermal imaging lenses. However, there is a lack of supporting color calibration equipment for fluorescent endoscopes, which are now widely used in hospitals and research institutes. Summary of the Invention

[0005] In response to the deficiencies in the prior art, the present invention provides a fluorescence color calibration card for endoscopes and a preparation method thereof. The fluorescence color calibration card for endoscopes can be used for parameter correction, operating status judgment and auxiliary adjustment of operating status under different fluorescence modes of fluorescence endoscope equipment.

[0006] One aspect of the present invention provides a fluorescent color calibration card for endoscopes, wherein the fluorescent color calibration card for endoscopes includes a color developing sheet, and the color developing sheet includes a fluorescent dye, an adsorption material and a substrate.

[0007] Preferably, the fluorescent dye is one or more of ultraviolet fluorescent dye, visible light fluorescent dye, and near-infrared fluorescent dye.

[0008] Preferably, the ultraviolet fluorescent dye is a small molecule fluorescent dye with an emission wavelength of 280 nm ≤ < 400 nm and / or an inorganic fluorescent dye with an emission wavelength of 280 nm ≤ < 400 nm, which can be exemplified by 1,8-naphthalene diimide;

[0009] Visible light fluorescent dyes are small molecule fluorescent dyes with an emission wavelength of 400 nm ≤ < 780 nm and / or inorganic fluorescent dyes with an emission wavelength of 400 nm ≤ < 780 nm, including but not limited to coumarin fluorescent dyes, fluorescein dyes, rhodamine dyes, pyrene fluorescent dyes, cyanine fluorescent dyes, BODIPY fluorescent dyes, carbon quantum dots, and zinc-dimethylpyridinamine (ZDPA) fluorescent dyes;

[0010] Near-infrared fluorescent dyes are small molecule fluorescent dyes with an emission wavelength of 780 nm ≤ ≤ 1700 nm and / or inorganic fluorescent dyes with an emission wavelength of 780 nm ≤ ≤ 1700 nm, including but not limited to cyanine fluorescent dyes, BODIPY fluorescent dyes, AIE fluorescent dyes, and DAD fluorescent dyes.

[0011] Preferably, the adsorption material is a material having one or more adsorption sites selected from -OH, -NH2, -SH, and Si-O-Si on its surface.

[0012] Preferably, the adsorption material is one or more of silica gel, alumina, aluminum silicate, polystyrene-divinylbenzene copolymer, and polydivinylbenzene-pyrrolidone copolymer.

[0013] Preferably, the substrate is one or more of glass, organic glass, and metal foil.

[0014] Preferably, the glass is one or more of borosilicate glass, quartz glass, and tempered glass;

[0015] The organic glass is one or more of polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), and polycarbonate (PC);

[0016] The metal sheet is one or more of an aluminum sheet, a titanium alloy sheet, an iron sheet, and a copper sheet.

[0017] Preferably, in the color-developing sheet, the adsorption material is uniformly attached to the substrate to form a thin layer with uniform texture, and the fluorescent dye is adsorbed in the adsorption material.

[0018] Preferably, the thickness of the adsorption material is 10 μm-1 cm, and the adsorption ratio of the fluorescent dye in the adsorption material is 0.0001-40 wt %.

[0019] Preferably, the thickness of the adsorption material is 0.2-1 mm, and the adsorption ratio of the fluorescent dye in the adsorption material is 0.1-10 wt %.

[0020] The thickness of the substrate is not limited and can be any thickness that can be attached to the adsorbent material. An example thickness is 0.25±0.05 mm.

[0021] Preferably, the method for preparing the color-developing sheet comprises the following steps:

[0022] The adsorption material is mixed evenly with water or an aqueous solution containing an adhesive, then poured on a substrate, smoothed, dried, and cut to a suitable size to form an adsorption sheet; the adsorption sheet is immersed in a fluorescent dye solution, taken out and dried to obtain a color-developing sheet.

[0023] The concentration of the aqueous solution containing the binder can be exemplified as 0.5 to 3 wt%.

[0024] Preferably, the fluorescent dye solution is formed by dissolving the fluorescent dye in a solvent, and the solvent includes one or more of water, methanol, ethanol, dichloromethane, chloroform, ethyl acetate, and petroleum ether.

[0025] Preferably, the concentration of the fluorescent dye solution is 0.05-1.0 mol / L.

[0026] More preferably, the concentration of the fluorescent dye solution is 0.1-0.5 mol / L.

[0027] More preferably, the concentration of the fluorescent dye solution is 0.11-0.25 mol / L.

[0028] Preferably, the fluorescent color calibration card for endoscope includes a substrate, a color developing sheet, and a plastic film; the color developing sheet is adhered to one side of the substrate, and the plastic film encapsulates the substrate and the color developing sheet.

[0029] The substrate can be hard cardboard, plastic sheet or organic glass sheet, and the thickness is not limited.

[0030] Another aspect of the present invention provides a method for preparing a fluorescent color calibration card for endoscopes, comprising the following steps: adhering a color-developing sheet to one side of a substrate, covering the substrate and the color-developing sheet with a plastic film, and heating the plastic film to encapsulate the substrate and the color-developing sheet. The heating temperature is 100-130°C.

[0031] Another aspect of the present invention provides an application of a fluorescence color calibration card for endoscopes, which is used for parameter correction of fluorescence endoscope equipment, operating status judgment of fluorescence endoscope equipment, and auxiliary adjustment of the operating status of fluorescence endoscope equipment under different fluorescence modes.

[0032] The term "enumeration" used herein is for illustrative purposes only and does not constitute a specific limitation to the present invention.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] (1) The present invention provides a fluorescent color calibration card for use with a fluorescent endoscope, which is the first of its kind in China and makes up for the current problem of a lack of matching color calibration cards for fluorescent endoscopes.

[0035] (2) The fluorescence color calibration card for endoscopes provided by the present invention can be used for parameter calibration of fluorescence endoscope equipment, determination of the operating status of fluorescence endoscope equipment, and auxiliary adjustment of the operating status of fluorescence endoscope equipment under different fluorescence modes;

[0036] (3) The present invention successfully prepares the core part of the fluorescent color calibration card for endoscopes - the color developing sheet - by attaching the adsorption material to the substrate with uniform thickness to form a thin layer with uniform texture, and then adsorbing the fluorescent dye into the adsorption material by soaking;

[0037] (4) The fluorescent color calibration card for endoscopes of the present invention is applicable to ultraviolet fluorescent dyes, visible light fluorescent dyes, and near-infrared fluorescent dyes, which greatly expands the scope of application;

[0038] (5) The fluorescent color calibration card for endoscopes of the present invention is sealed with a plastic film to isolate the air, reduce the quenching of the fluorescent dye, and increase the service life of the fluorescent color calibration card for endoscopes. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic structural diagram of a fluorescent color calibration card for endoscopes in a preferred embodiment of the present invention;

[0040] Figure 2 This is a comparison diagram of fluorescence imaging after the fluorescence calibration cards for endoscopes of Example 1, Example 16, Example 17, Example 18, and Example 20 absorb light waves;

[0041] Figure 3 This is a graph showing the relationship between fluorescence intensity and fluorescent dye concentration for a fluorescence calibration card used in endoscopes.

[0042] Figure 1 In the invention, 1. color developing sheet; 2. substrate; 3. plastic film. DETAILED DESCRIPTION

[0043] The technical scheme of the present invention is further described below by specific examples and accompanying drawings. It should be understood that the specific embodiments described herein are only used to help understand the present invention and are not intended to be limiting of the present invention. Unless otherwise specified, the raw materials used in the embodiments of the present invention are all commonly used raw materials in the art, and the methods used in the embodiments are all conventional methods in the art.

[0044] Figure 1This is a structural diagram of a fluorescent color calibration card for endoscopes in a preferred embodiment of the present invention, which includes a substrate 2, a color-developing sheet 1, and a plastic film 3. The plastic film 3 encapsulates the substrate 2 and the color-developing sheet 1 to seal and isolate the air.

[0045] Example 1

[0046] The color developing sheet of the fluorescent color calibration card for endoscope of this embodiment is prepared by the following steps:

[0047] Silica gel and an equal amount of CMC aqueous solution (concentration of 1wt%) were mixed evenly and stirred continuously until all the silica gel was completely wetted, and then ultrasonic treatment was performed to remove bubbles. A cleaned and dried aluminum sheet was laid flat, and an appropriate amount of silica gel and CMC mixture was poured on the substrate. The sheet was then scraped flat, dried, and cut into appropriate sizes to obtain an adsorption sheet. The thickness of the adsorption material was 0.4mm.

[0048] ICG dye was prepared into a 0.1 mol / ml ethanol solution, the adsorption sheet was immersed in the ICG solution for 5 seconds, then taken out and dried in a vacuum oven to obtain a color-developed sheet.

[0049] The color-developing sheet is adhered to one side of a substrate (hard cardboard), the substrate and the color-developing sheet are covered with a plastic film, and the plastic film is heated at 120° C. to seal the substrate and the color-developing sheet to obtain a fluorescent color calibration card for endoscopes.

[0050] The raw material compositions of the color-developing sheets of the fluorescent color calibration cards for endoscopes of Examples 2 to 33 are shown in Table 1 below. The fluorescent color calibration cards for endoscopes are obtained using the same preparation method as in Example 1.

[0051] Table 1 Composition of raw materials in Examples 1 to 33

[0052]

[0053]

[0054] Figure 2 This is a comparison diagram of fluorescence imaging after the fluorescence calibration card for endoscopes of Example 1, Example 16, Example 17, Example 18, and Example 20 absorbs light waves. Figure 3 This is a relationship diagram between the fluorescence intensity of the fluorescent color calibration card for endoscopes and the concentration of fluorescent dyes. As can be seen from the figure, the fluorescent color calibration card for endoscopes provided by the present invention emits a high fluorescence intensity after absorbing light waves, especially when the concentration of the ICG fluorescent dye solution is in the range of 0.1-0.5 mol / L, and especially when the concentration of the fluorescent dye solution is in the range of 0.11-0.25 mol / L, the fluorescence intensity is even higher.

[0055] Finally, it should be noted that the specific embodiments described herein are merely illustrative of the spirit of the present invention and are not intended to limit the manner in which the present invention is intended to be implemented. Persons skilled in the art may make various modifications, additions, or substitutions to the described embodiments, and it is not necessary or possible to provide a comprehensive list of all possible embodiments. However, any obvious changes or modifications arising from the essential spirit of the present invention remain within the scope of protection of the present invention, and interpreting them as any additional limitations would be contrary to the spirit of the present invention.

Claims

1. An application of a fluorescent color calibration card for endoscope, characterized in that: The fluorescence color calibration card for endoscope is used for parameter correction of fluorescence endoscope equipment, operation status judgment of fluorescence endoscope equipment or auxiliary adjustment of operation status of fluorescence endoscope equipment under different fluorescence modes; The fluorescent color calibration card for endoscope includes a color developing sheet, and the color developing sheet includes a fluorescent dye, an adsorption material and a substrate; The fluorescent dye is a near-infrared fluorescent dye, which is a small molecule fluorescent dye with an emission wavelength of 780 nm ≤ ≤ 1700 nm and / or an inorganic fluorescent dye with an emission wavelength of 780 nm ≤ ≤ 1700 nm; The adsorption material is silica gel; In the color-developing sheet, the adsorption material is attached to the substrate, and the fluorescent dye is adsorbed in the adsorption material; The preparation method of the color developing sheet comprises the following steps: The adsorption material is mixed evenly with water or an aqueous solution containing an adhesive, then poured onto a substrate, smoothed, dried, and cut into a suitable size to form an adsorption sheet; the adsorption sheet is immersed in a 0.125-0.25 mol / L fluorescent dye solution, taken out and dried to obtain a color-developing sheet; The fluorescent dye solution is formed by dissolving the fluorescent dye in a solvent, and the solvent includes one or more of water, methanol, ethanol, dichloromethane, chloroform, ethyl acetate, and petroleum ether.

2. The use according to claim 1, characterized in that The substrate is one or more of glass, organic glass, and metal foil.

3. The use according to claim 2, characterized in that The fluorescent dye is ICG, IR-780, IR-783 or IR-808.

4. The use according to claim 2, characterized in that The glass is one or more of borosilicate glass, quartz glass, and tempered glass.

5. The use according to claim 2, characterized in that The organic glass is one or more of polydimethylsiloxane, polymethyl methacrylate, and polycarbonate.

6. The use according to claim 2, characterized in that The metal sheet is one or more of an aluminum sheet, a titanium alloy sheet, an iron sheet, and a copper sheet.

7. The use according to claim 1, characterized in that The thickness of the adsorption material is 10 μm-1 cm, and the adsorption ratio of the fluorescent dye in the adsorption material is 0.01-40 wt %.

8. The use according to claim 1, characterized in that The invention comprises a substrate, a color-developing sheet and a plastic film; the color-developing sheet is adhered to one side of the substrate, and the plastic film seals the substrate and the color-developing sheet.

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