A biological probiotic factor composition and its application in anti-dandruff cosmetics

Abstract

The present invention belongs to the field of daily chemicals, and is specifically a biological probiotic factor composition and its application in anti-dandruff cosmetics. The present invention is composed of the following components by weight: (1) 40-58% of yeast fermentation product extract; (2) 20-36% of biological marine components; (3) 0.1-0.5% of pH buffer, (4) 0.4% of sodium benzoate and the remainder of deionized water. The present invention re-establishes the scalp microecological system through two technical methods of biofilm sterilization and oil control, so that the scalp returns to a healthy state and plays a lasting anti-dandruff effect.

Description

Technical Field

[0001] The invention belongs to the field of daily chemicals, and specifically relates to a biological probiotic factor composition and its application in anti-dandruff cosmetics. Background Art

[0002] Dandruff has always been a sub-healthy state of the scalp that causes trouble to people. Technicians have been working hard to reduce the occurrence of dandruff through various technical means. In the past, the commonly used technical means was to use famous antidandruff agents that have an inhibitory effect on Malassezia, such as zinc pyrithione ZPT and pyridone ethanolamine salt OCT, to inhibit bacteria and reduce the occurrence of dandruff. However, although the effects of ZPT and OCT are obvious to all, clinical findings show that there is still a problem of drug resistance after long-term use, especially in terms of mildness and durability of anti-dandruff. In addition, the "bactericidal" effect of chemical antidandruff agents often destroys the scalp microecology. The imbalance of scalp flora will cause the loss of the scalp's barrier function, and the oil secretion of the scalp cannot be controlled. Dandruff recurs, causing a lot of trouble for people.

[0003] According to research, microorganisms, which have an absolute dominant advantage, can be killed by traditional bactericides used for personal care, but are difficult to be eradicated, and it is difficult to affect or remove the microbial film derived from microorganisms. In addition, the balance of microecology involves the "breaking" and "establishing" of the microbial balance mechanism. "Breaking" means breaking the balance of the host and destroying the original basis for survival, and "establishing" means re-establishing the "micro-ecological system". The microbial film is a powerful network evolved from polysaccharides, proteins, etc. secreted by microorganisms. It is a protective umbrella and nutrient supplier for microorganisms, providing protection for microbial growth. Once the microbial film is formed, it is often difficult to destroy or remove. Some bactericides and anti-dandruff agents such as ZPT (zinc pyrithione) and OCT (pyridone ethanolamine salt) can reduce a certain amount of microbial counts, but it is difficult to remove the microbial film derived from a variety of microorganisms. Therefore, it is necessary for the present invention to adopt non-chemical "sterilization" means to open up a new technical route, such as by adopting a technical method combining the removal of scalp microbial film and the regulation of scalp oil secretion to achieve the purpose of scalp dandruff. Summary of the invention

[0004] The purpose of the present invention is to provide a biological probiotic factor composition comprising a yeast fermentation product extract and a biological marine component. The biological probiotic factor composition can remove scalp microbial membranes and regulate scalp oil secretion to achieve the purpose of scalp dandruff removal.

[0005] The invention also provides application of the biological probiotic factor composition in preparing anti-dandruff cosmetics.

[0006] The present invention discloses a biological probiotic factor composition, which comprises a yeast fermentation product extract and a biological marine component, and specifically consists of the following components:

[0007] (1) 40-58% yeast fermentation product extract; (2) 20-36% biological marine component; (3) 0.1-0.5% pH buffer, wherein the pH buffer is citric acid and sodium citrate, and the weight ratio of citric acid to sodium citrate is 1:2 to 2:1; (4) 0.4% sodium benzoate; (5) the balance is water;

[0008] The yeast fermentation product extract is composed of 30-50% yeast fermentation extract, 0.03% polylysine and the remainder deionized water, wherein the yeast fermentation extract contains ≥95% bioglycolipids, and the yeast fermentation extract is obtained by fermenting and purifying vegetable oil and sugar through a Torulopsis strain.

[0009] The yeast fermentation product extract of the present invention can effectively dissolve and remove the scalp microbial biofilm, thereby playing the role of sterilization and dandruff removal.

[0010] The biological marine component is an extract of a fermentation product of Alteromonas, and the biological marine component contains 25% of the extract of a fermentation product of Alteromonas, 0.5% of sodium salicylate and 74.5% of water.

[0011] The biological probiotic factor composition of the present invention contains 0.1-0.5% of pH buffer to adjust the pH to 4.5-5.5;

[0012] The biological probiotic factor composition of the present invention contains 0.4% sodium benzoate preservative.

[0013] Advantageous Effects of the Invention

[0014] The scalp anti-dandruff technology of the present invention is different from the traditional anti-dandruff mechanism. It adopts a non-"bacterial" method, using scalp probiotic factor fermentation product extracts, such as yeast fermentation product extracts, to dissolve and remove scalp biofilms to remove dandruff, and using the scalp oil control technology of biological marine ingredients (Pseudoalteromonas fermentation product extracts) to reduce dandruff. Therefore, the technical feature of the present invention is to re-establish the scalp micro-ecological system through the two technical methods of biofilm sterilization and oil control, so that the scalp returns to a healthy state and has a lasting anti-dandruff effect. Compared with traditional anti-dandruff agents ZPT and OCT, it has a good anti-dandruff effect, avoids the recurrence of dandruff, and is more gentle.

[0015] The biological marine ingredient of the present invention can minimize the accumulation of sebaceous gland lipids and can play a role in regulating the scalp cortex and controlling oil. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 -4 is a microfluidic tube experiment. Among them, Figure 1 The microbial film / biofilm is attached to the surface of the microfluidic tube; Figure 2 Effectively remove biofilm attached to microfluidic surfaces for yeast fermentation extracts; Figure 3 Because SDS cannot effectively remove biofilm attached to the microfluidic surface; Figure 4 In order to continue to observe the surface of the control tube through which the SDS microflow passes, the microbial film can still grow again after 36 hours.

[0017] Figure 5 This is a schematic diagram of the six areas of the scalp.

[0018] Figure 6 For the most severe case of dandruff, the photos before and after Example IV were used. 6a, 6b, 6c, and 6d used the dandruff photos before, on the 14th day, on the 28th day, and on the 42nd day of Example IV, respectively. DETAILED DESCRIPTION

[0019] The term "microsystem" in the present invention refers to a unified biological system (biosystem) formed in a certain structured space with normal microorganisms and their host tissues and cells and their metabolites as the environment, which can independently exchange substances, energy and information with each other during the long-term evolution process; that is, it is composed of two components: normal microorganisms and their host microenvironment.

[0020] The term "BIOSCALP" in the present invention refers to substances that have a conditioning effect on the establishment of a healthy microecological system on the scalp, such as the biological surface activity of yeast fermentation product extract, which can dissolve and remove microbial biofilms and play a role in removing dandruff; and the biological marine ingredient Pseudoalteromonas fermentation product extract, which can control oil and regulate lipid accumulation. The combination of the above two biological probiotic factors helps to rebuild and balance the scalp microecology, thereby playing a lasting role in removing dandruff.

[0021] The technical indicators of the yeast fermentation product extract of the invention are as follows: the appearance is amber viscous liquid, has a characteristic fermentation smell, a drying loss of 45.0-55.0%, a pH value of 4.3-6.3 (5% aqueous solution, 25°C), and sodium chloride ≤4.

[0022] The yeast fermentation extract of the present invention is obtained by fermenting and purifying vegetable oil and sugar with a strain of Candida albicans. The yeast fermentation extract contains ≥95% of bioglycolipid-type biosurfactant, which can effectively decompose and remove the microbial film (biofilm) attached to the scalp through its unique amphiphilic molecular group structure, thereby playing a role in sterilization and dandruff removal. This is completely different from the bactericidal and antibacterial mechanism of traditional chemical antidandruff agents. In addition, the yeast fermentation product extract itself comes from organisms, is completely biodegradable, and is very mild.

[0023] Microbial film removal simulation test method:

[0024] The microfluidic tube method was used to generate a microbial film on the tube wall. The microbial film (biofilm) was attached to the surface of the microfluidic tube. Then, 0.1% yeast fermentation product extract and 0.1% SDS (mass percentage) were taken respectively. Then, the prepared yeast fermentation product extract and SDS were passed through different microfluidic tube walls with biofilms attached. After 5 minutes, the attachment state of the microorganisms in the tube wall was observed. The results were shown in Figure 2. Figure 1 , Figure 2 and Figure 3 .

[0025] (Microbial film / bacterial film attached to the microfluidic surface) Yeast fermentation extract effectively removes SDS (K12) cannot effectively remove SDS (K12) The microbial film attached to the microfluidic surface) The microbial film attached to the microfluidic surface can still grow again

[0026] Brief description of the preparation process of yeast fermentation extract:

[0027] The present invention adopts a biological fermentation and purification process, using vegetable oil and sugar as raw materials, and fermenting them with a Torulopsis candida strain, including two steps of fermentation and purification. The process of separating and purifying the desired fermentation product such as biological glycolipid from the fermentation broth includes mash transportation, filtration and impurity removal, gel filtration, precipitation separation, and subsequent solvent extraction, evaporation, and crystallization, and finally obtaining a yeast fermentation extract with a biological glycolipid content greater than 95%.

[0028] The present invention, wherein the biological marine component is composed of 25% of an extract of a fermentation product of Alteromonas, 0.5% of sodium salicylate and 74.5% of water, and the biological marine component, trade name is Seasc alp TM , the manufacturer is American Lubricant.

[0029] The biological marine ingredients of the present invention are capable of minimizing the accumulation of sebaceous gland lipids.

[0030] Test method: Human primary sebaceous gland cells were used, and α-MSH (basal control), 1mg / mL and 10mg / mL concentrations of SeascalpTM Bio-marine ingredients were used for treatment and cultured for four days. TM The results showed that the biological marine ingredient Seascalp TM Significantly inhibits the accumulation of sebaceous gland lipids. In addition, Seascalp TM Bio-marine ingredients also have an inhibitory effect on Malassezia furfur, helping to reduce dandruff.

[0031] The present invention discloses a biological probiotic factor composition, which contains 0.1-0.5% of a pH buffer, wherein the pH buffer is citric acid and sodium citrate, and the weight ratio of the citric acid to the sodium citrate is 1:2 to 2:1. Finally, the pH is adjusted to 4.5-5.5, and the pH buffer helps to maintain the stability of the biological probiotic factor.

[0032] Preparation method of biological probiotic factors:

[0033] (1) 0.4% sodium benzoate is added to water and stirred until dissolved. (2) 40-58% yeast fermentation product extract and 20-36% biological marine component are added to (1) at room temperature and stirred to dissolve. (3) 0.1-0.5% pH buffer is added to the mixed solution of (1) and (2) and the pH is adjusted to 4.5-5.5.

[0034] The invention discloses an application of the biological probiotic factor composition in the preparation of anti-dandruff cosmetics.

[0035] The embodiment of the invention discloses the application of a biological probiotic factor composition in the preparation of anti-dandruff cosmetics.

[0036] The biological probiotic factor composition described in the present invention can be applied to and added to different dosage forms of products such as anti-dandruff essence, anti-dandruff conditioner, and anti-dandruff shampoo. The embodiments of the present invention only use the addition of anti-dandruff shampoo as a reference, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0037] Anti-dandruff shampoo examples are shown in Table 1

[0038] Table 1 Examples I-VII

[0039]

[0040] ＊The composition of the bio-probiotic factor composition includes: (1) 55% yeast fermentation product extract; (2) 35% Pseudoalteromonas fermentation product extract; (3) 0.4% pH buffer, wherein the pH buffer is citric acid and sodium citrate, and the weight ratio of citric acid to sodium citrate is 1:1.2, and finally the pH of the composition is adjusted to 5.5; (4) 0.4% sodium benzoate, and (5) the balance is water.

[0041] How to make anti-dandruff shampoo:

[0042] Preparation method of anti-hair loss shampoo:

[0043] (1) Add an appropriate amount of citric acid to sufficient deionized water, heat to dissolve, then add 3g of cationic guar gum (guar hydroxypropyltrimonium chloride), stir to dissolve, then add 170.0g of sodium laureth sulfate, 30.0g of cocamidopropyl betaine, 30.0g of decyl glucoside, 1.0g of disodium EDTA, 5.0g of betaine and 1.5g of salicylic acid, stir to dissolve. (2) Cool (1) to 60°C, then add 5g of ZPT (if contained) and 5g of OCT (if contained), as well as an appropriate amount of preservatives, flavors, pigments and salt. Cool to 39°C, then add 5g, 10g, 15g, or 20g of the bio-beneficial factor composition. ＊ (If contained) stir evenly, finally add appropriate amount of citric acid / sodium citrate buffer (about 0.4%) and stir, and adjust the pH value of the system to 5.5. Finally, stir evenly and discharge.

[0044] Efficacy verification

[0045] Clinical evaluation of anti-dandruff efficacy

[0046] Dandruff population selection: Experts visually graded and evaluated the degree of dandruff adhesion in 6 areas of the scalp (see Figure 5 ), and screened 210 volunteers with moderate to severe dandruff, aged 18-60 years old.

[0047] 0: No dandruff

[0048] 1-2: small, gray powdery, rough flakes

[0049] 3-4: Small to medium flakes

[0050] 5-6: Loose, discontinuous, large and thin flakes

[0051] 7-8: Flakes stuck together

[0052] 9-10: Thick white or yellow flakes close to the scalp

[0053] The shampoo anti-dandruff evaluation method:

[0054] Volunteers aged 18-60 with moderate to severe dandruff were selected. The scalp was photographed before using the product and the test was divided into 7 groups, 30 people in each group. The usage method and instructions for each group are shown in Table 2.

[0055] The evaluation options for anti-dandruff effect are: significant (clean scalp, dandruff is almost gone), general (a little dandruff is still observed) and ineffective (dandruff is the same as before using the product). The evaluation results are shown in Table 3.

[0056] Instructions for use - Table 2

[0057] Table 2

[0058]

[0059] Survey on the evaluation of the sample anti-dandruff by the trial users - Table 3 (effectiveness%, statistical effective number of people including significant and general)

[0060] Table 3

[0061]

[0062] The formulas of Examples I-IV corresponding to the first, second, third and fourth groups contain 0.5%, 1.0%, 1.5% and 2.0% of the anti-dandruff agent of the biological probiotic factor composition of the present invention respectively;

[0063] The formulas of Example V and Example VI corresponding to the fifth and sixth groups contain 0.5% ZPT and 0.5% OCT chemical anti-dandruff agents, respectively;

[0064] Example VII corresponding to the seventh group is a blank control example of the present invention, which does not contain the above-mentioned biological probiotic factor composition, ZPT and OCT anti-dandruff ingredients.

[0065] Table 3 Anti-dandruff evaluation results show:

[0066] (1) Example VII (blank control) corresponding to the seventh group, the anti-dandruff effectiveness rate on the 28th day and the 42nd day was 40%.

[0067] (2) The anti-dandruff efficacy of the formulations of Examples I to IV containing different percentages of the bio-probiotic factor composition on the 14th day and the 28th day was greater than 70%, with the highest being 90% (see Example IV, see Figure 6 ); The anti-dandruff effectiveness on the 42nd day was still maintained at a minimum of 67% and a maximum of 87%, indicating that Examples I-IV maintained a relatively high anti-dandruff effectiveness after being used for 28 days + 14 days after the end of use.

[0068] (3) The dandruff removal efficacy of the fifth and sixth groups containing the chemical antidandruff agent ZPT or OCT, corresponding to the Example V and Example VI, was 80% at the lowest and 93% at the highest on the 14th and 28th days; the dandruff removal efficacy was 47% at the lowest and 50% at the highest on the 42nd day. This indicates that the dandruff removal efficacy of Example V and Example VI was greatly reduced after 28 days of use + 14 days after the end of use, and was only slightly higher than that of the blank control Example VII of the present invention.

[0069] The above analysis results show that the anti-dandruff shampoo containing the biological probiotic factor composition of the present invention has a slightly lower anti-dandruff effect than the shampoo containing the chemical anti-dandruff agent ZPT or OCT, but basically maintains a similar level of dandruff removal rate; while the embodiment containing the chemical anti-dandruff agent ZPT or OCT did experience a significant rebound of dandruff on the 14th day after 28 days of use. The anti-dandruff effectiveness of the anti-dandruff shampoo embodiment containing the biological probiotic factor composition of the present invention on the 42nd day still remains at a relatively high anti-dandruff effectiveness. This shows that the shampoo embodiment containing biological probiotic factors can remove dandruff for a long time and prevent the recurrence of dandruff, which depends on the reconstruction and balance of the scalp microecology.

[0070] Evaluation method for the oil control effect of the shampoo:

[0071] Use Sebumeter SM815 skin oil tester to test the scalp oil value and calculate the oil removal rate.

[0072] The volunteers and the method of use are the same as those in Table 2: The volunteers in the shampoo anti-dandruff evaluation test were used to test their scalp oil values ​​on day 0, day 28, and day 42 and calculate the oil removal rate. The evaluation results are shown in Table 4.

[0073] Table 4

[0074]

[0075] The results in Table 4 show that Examples I-IV containing different percentages of the bio-probiotic factor composition show higher and more stable oil removal rates.

Claims

1. A biological probiotic factor composition, characterized in that: The biological probiotic factor comprises yeast fermentation product extract and biological marine ingredients, and is specifically composed of the following components in weight ratio: (1) Yeast fermentation product extract 40-58%; (2) biological marine components 20-36%; (3) 0.1-0.5% pH buffer, wherein the pH buffer is citric acid and sodium citrate, and the weight ratio of citric acid to sodium citrate is 1:2 to 2:1; (4) Sodium benzoate 0.4%; (5) Remaining amount of water; The yeast fermentation product extract component is composed of 30-50% of the total weight of the yeast fermentation product extract, 0.03% of polylysine, and the balance of deionized water, wherein the yeast fermentation extract contains ≥95% of bioglycolipids; The biological marine component is an extract of an Alteromonas fermentation product, and the extract is composed of 25% of the extract of an Alteromonas fermentation product, 0.5% of sodium salicylate and 74.5% of water.

2. The composition according to claim 1, wherein the bio-probiotic factor composition is used in the preparation of anti-dandruff cosmetics.

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