Methods and kits for detecting the presence or absence and / or amount of enterobacteriaceae bacteria in a food or beverage sample, an environmental sample, or a biological sample

By combining antigen-antibody reactions with universal and specific antibodies, the problems of long detection time and insufficient selectivity in existing technologies have been solved, enabling rapid and effective detection of multiple genera of Enterobacteriaceae and improving the sensitivity and accuracy of detection.

CN116762009BActive Publication Date: 2026-06-09ASAHI KASEI KOGYO KABUSHIKI KAISHA
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Patent Information

Application Number
CN202280009286.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2021-01-15
Filing Date
2022-01-14
Publication Date
2026-06-09
Estimated Expiration
2042-01-14

AI Technical Summary

Technical Problem

Existing technologies for detecting Enterobacteriaceae in food, beverage, environmental, and biological samples suffer from problems such as long detection times, insufficient selectivity, and difficulty in distinguishing eukaryotic cells, making it impossible to quickly and effectively detect multiple genera of Enterobacteriaceae.

Method used

An antigen-antibody reaction-based method is used to simultaneously detect Enterobacteriaceae bacteria from multiple genera in a sample by combining universal and specific antibodies. The presence and quantity of these bacteria are identified and determined through antigen-antibody reactions.

Benefits of technology

It enables the simple and effective detection of the presence and quantity of Enterobacteriaceae bacteria from multiple genera in a short time, improving the sensitivity and accuracy of detection and reducing the false positive rate.

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Abstract

Provided is a method for detecting the presence or absence and / or amount of Enterobacteriaceae bacteria in a food or drink sample, an environmental sample, or a biological sample, in a short period of time, easily and efficiently. The method includes a step of simultaneously detecting the presence or absence and / or amount of at least two or more different genera of Enterobacteriaceae bacteria in a sample based on an antigen-antibody reaction.
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Citation Information

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