A forced swimming system for experimental small animals

Abstract

The present invention provides a forced swimming system for small animals for experiment, comprising: an automatic water adding structure for automatically adding water to adjust the water depth; an automatic water temperature regulating structure for automatically raising the water temperature to adjust the water temperature; an automatic water flow regulating structure for automatically adjusting the water flow by surging water; an automatic video monitoring structure for automatically monitoring the forced swimming conditions of the small animals; an automatic fishing structure for automatically fishing the small animals when the animals are analyzed to be in a state of exhaustion; a function for automatically fishing the small animals after they have been fished out and left on the water surface for a period of time, and then returning them to the water to continue the forced swimming experiment after they have recovered; a function for automatically fishing the small animals when the experiment duration has expired; an automatic water draining structure for automatically draining water; and an automatic cleaning and disinfection structure for automatically cleaning and disinfecting. The present invention can achieve comprehensive, intelligent, and automated control of the entire small animal forced swimming experiment process.

Description

Technical Field

[0001] The invention relates to the technical field of forced swimming of experimental animals, in particular to a forced swimming system for experimental small animals. Background Art

[0002] Forced swimming experiments involve placing animals in a confined swimming apparatus at a fixed water level. This device, designed to induce stress and fatigue in animals, creates a forced swimming environment.

[0003] Existing forced swimming devices for experimental animals can only achieve some automated functions, but many functions still require manual intervention, which brings great inconvenience to experiments and is time-consuming and labor-intensive. Furthermore, existing forced swimming devices for experimental animals cannot intelligently adjust functions such as water temperature and water flow, which is not conducive to establishing a better forced swimming environment for experimental animals. Based on this, the inventors of the present invention have designed a forced swimming system for experimental small animals that can achieve comprehensive, intelligent, and automated control of the entire experimental process. Summary of the Invention

[0004] The present invention aims to solve the problems and shortcomings of the prior art and provides a forced swimming system for experimental small animals.

[0005] The present invention solves the above technical problems through the following technical solutions:

[0006] The present invention provides a forced swimming system for small animals in an experiment, which is characterized in that it includes four support columns located at four corners, the top supports of the four support columns are fixed with a box body, the box body is provided with a plurality of cylindrical placement cavities, each of the cylindrical placement cavities is fixed with a heat-conductive cylindrical experimental cylinder with open top and bottom, each of the cylindrical experimental cylinders is correspondingly provided with a lower computer, and each of the lower computers is communicatively connected to the upper computer.

[0007] Each of the cylindrical test cylinders is correspondingly provided with a water inlet pipe, one end of each of the water inlet pipes passes through the box and the corresponding cylindrical test cylinder and is placed on the upper part of the corresponding cylindrical test cylinder, the other end of each of the water inlet pipes is placed outside the box, each of the water inlet pipes is provided with an electric water inlet valve and the electric water inlet valve is placed outside the box, and the inner wall of each of the cylindrical test cylinders is evenly spaced along the vertical direction from bottom to top with multiple liquid level sensors forming a row of liquid level sensors.

[0008] The outer wall of each cylindrical experimental tube is wound with a spiral electric heating wire from bottom to top, and the inner bottom of each cylindrical experimental tube is fixed with a temperature sensor.

[0009] Electric propellers are respectively fixed on opposite sides of the inner bottom of each cylindrical test tube.

[0010] A camera is fixed on the inner top of each cylindrical experimental tube, and the camera faces the corresponding cylindrical experimental tube.

[0011] The inner wall of each cylindrical experimental tube is roughened from the middle position to a position a certain distance from the top, so that the experimental animals can climb on it.

[0012] An electric push rod is fixed to the outside of the bottom of the box and directly below the middle position of each cylindrical experimental tube. A sleeve is fixed to the middle position of the inner bottom of each cylindrical experimental tube. The push rod end of each electric push rod passes through the bottom of the box and is placed in the corresponding sleeve. A round pedestal seat is fixed to the top of the push rod end of each electric push rod. A plurality of L-shaped connecting rods are hinged on the surface of each round pedestal seat along the circumferential direction. The bottom of the cross bar of one of the L-shaped connecting rods is fixed with a limited Position sensors, small counterweights are fixed at the bottom of the cross bar ends of these L-shaped connecting rods, and animal fishing nets are fixed on the tops of these L-shaped connecting rods. When these L-shaped connecting rods are in the initial state, the vertical rods of these L-shaped connecting rods are placed in the corresponding sleeves, and the cross bars of these L-shaped connecting rods are placed on the outer edges of the tops of the sleeves, and the corresponding animal fishing nets are in a contracted state. When these L-shaped connecting rods are in the expanded state, the corresponding animal fishing nets are in an expanded state, and the expanded animal fishing nets cover the lateral surfaces of the corresponding cylindrical experimental tubes.

[0013] The upper computer is used to send a water depth control instruction containing a set water depth value to the corresponding lower computer, and the corresponding lower computer is used to start the corresponding row of liquid level sensors after receiving the water depth control instruction, obtain the current water depth value in the corresponding cylindrical test cylinder based on the corresponding row of liquid level sensors, and judge whether the current water depth value reaches the corresponding set water depth value. If the answer is yes, an instruction that the water depth has been controlled is sent to the upper computer, and if the answer is no, the corresponding water inlet electric valve is controlled to open, and water is added to the corresponding cylindrical test cylinder through the corresponding water inlet pipe until the current water depth value reaches the corresponding set water depth value, and the corresponding water inlet electric valve is controlled to close.

[0014] The upper computer is used to send a water temperature control instruction containing a set water temperature value to the corresponding lower computer after receiving the instruction that the water depth has been controlled. The corresponding lower computer is used to start the corresponding temperature sensor after receiving the water temperature control instruction, obtain the current water temperature value in the corresponding cylindrical experimental cylinder based on the corresponding temperature sensor, and judge whether the current water temperature value reaches the corresponding set water temperature value. If the answer is yes, the instruction that the water temperature has been controlled is sent to the upper computer. If the answer is no, the corresponding spiral electric heating wire is controlled to start, and the water in the corresponding cylindrical experimental cylinder is heated until the current water temperature value reaches the corresponding set water temperature value, and the spiral electric heating wire is controlled to pause.

[0015] The upper computer is used to send a water flow surge control instruction containing a set water flow intensity to the corresponding lower computer after receiving an instruction that the water temperature has been controlled. The corresponding lower computer is used to start the corresponding electric propeller in the cylindrical test tube after receiving this water flow surge control instruction and control the corresponding electric propeller to rotate at a speed matching the set water flow intensity.

[0016] The upper computer is used to send a video monitoring instruction to the corresponding lower computer, and record the start time of the animal experiment at this time. The corresponding lower computer is used to start the corresponding camera after receiving the video monitoring instruction to shoot the forced swimming situation of the experimental small animal in the corresponding cylindrical experimental tube and transmit it to the upper computer. The upper computer is used to analyze the current experimental behavior of the experimental small animal based on the forced swimming situation of the experimental small animal using image processing technology. The experimental behavior includes swimming behavior, climbing behavior, stationary behavior and exhaustion behavior. When it is judged that the current experimental behavior is exhaustion behavior, an animal fishing instruction is sent to the corresponding lower computer, and the time point when the animal is fished out at this time is recorded, and the number of swimming behaviors and the corresponding duration, the number of climbing behaviors and the corresponding duration, and the number of stationary behaviors between the time points when the animal is fished out are recorded. and the corresponding duration, the corresponding lower computer is used to start the corresponding electric push rod after receiving the corresponding animal fishing instruction, control the corresponding electric push rod to push the L-shaped connecting rod thereon to move upward, and the L-shaped connecting rods gradually expose the corresponding sleeves, and the L-shaped connecting rods are unfolded under the action of the corresponding small counterweight blocks, and the corresponding animal fishing net is unfolded to catch the experimental small animals. The animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, controls the electric push rod to pause, and judges whether the time the experimental small animals stay above the water surface reaches the set stay time. When the judgement is yes, start the corresponding electric push rod, control the corresponding electric push rod to reset, and pause the corresponding electric push rod when receiving the limit information from the limit sensor, and put the experimental small animals into the water again and continue to force them to swim, and repeat the shooting, scooping and putting them into the water operations.

[0017] The upper computer is used to record the end time of the animal experiment when the experiment time reaches the set experiment time, and send an animal fishing instruction to the corresponding lower computer. The corresponding lower computer is used to start the corresponding electric push rod after receiving the corresponding animal fishing instruction, control the corresponding electric push rod to push the L-shaped connecting rod thereon to move upward, and the corresponding animal fishing net is unfolded to catch the experimental small animals. After the animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, the electric push rod is controlled to pause, and an experiment ends.

[0018] Furthermore, a drain pipe is inserted and fixed at the bottom of the box body and at the bottom of each cylindrical experimental tube, and a drain electric valve is provided on each drain pipe and located outside the box body.

[0019] The upper computer is used to send a water discharge control instruction to the corresponding lower computer after the end of an experiment. The corresponding lower computer is used to start the corresponding water discharge electric valve after receiving the water discharge control instruction, so that the water in the corresponding cylindrical experimental tube is discharged through the corresponding water discharge pipe, and control the corresponding water discharge electric valve to close after the water discharge time reaches the set water discharge time.

[0020] Furthermore, two driving motors are fixed to the outside of the bottom of the box and directly below each cylindrical experimental cylinder. The driving ends of the two driving motors are passed through the bottom of the corresponding cylindrical experimental cylinder and are fixed with screws. The two screws are respectively placed on opposite sides of the inner wall of the corresponding cylindrical experimental cylinder. Each screw is sleeved with a nut. A base with a center hole is fixed between the two nuts. A bearing mounting seat coaxially arranged with the center hole is provided in the base. A bearing is installed in the bearing mounting seat. A bearing ring cover is provided at the bottom of the base through a sealing ring cover. The bearing ring cover is located directly below the bearing. The bearing is sealed in the base by the bearing ring cover, and the base and the bearing ring cover are fixed by bolts.

[0021] Liquid inlets for an external cleaning and disinfecting liquid tank are respectively provided on the base and on both sides of the bearing in the vertical direction. A rotatable shaft is connected to the bearing through the shaft. A circle of inlet holes and a circle of outlet holes are vertically spaced apart on the rotatable shaft and below the bearing ring cover. The circle of inlet holes are inlet holes evenly spaced along the circumferential direction, and the circle of outlet holes are outlet holes evenly spaced along the circumferential direction. The circle of inlet holes is located above the circle of outlet holes, and the inlet holes and outlet holes correspond one to one. An on-axis channel connecting the inlet hole and the corresponding outlet hole is provided in the rotatable shaft, and a blocking cover is fixed to the bottom of the rotatable shaft.

[0022] A liquid collection plate is sleeved on the rotatable shaft, and the top of the liquid collection plate is screwed to the bottom of the base, and the liquid collection cavity of the liquid collection plate corresponds to a circle of inlet holes, so that the cleaning and disinfecting liquid entering the liquid inlet first flows into the liquid collection cavity, and then flows into the channel on the shaft through a circle of inlet holes. A rotatable spray ring body is sleeved and fixed on the rotatable shaft, and the rotatable spray ring body is located between the liquid collection plate and the blocking cover. A connecting pipe connected to each outlet hole in a circle of outlet holes is opened on the inner wall of the rotatable spray ring body along the diameter direction of the rotatable spray ring body, and the connecting pipe is connected to each outlet hole in a circle. The outer wall periphery of the rotatable spray ring body is provided with liquid spray oblique outlet holes connected to the corresponding connecting pipes, and the bottom end of the rotatable spray ring body is provided with a plurality of liquid spray bottom outlet holes connected to the corresponding connecting pipes.

[0023] The upper computer is used to send a cleaning and disinfection control instruction to the corresponding lower computer after an experiment is completed. The corresponding lower computer is used to control the corresponding two drive motors to start and the corresponding water discharge electric valve to open after receiving the cleaning and disinfection control instruction. The two drive motors drive the corresponding screw rods to rotate, and the two nuts gradually move downward along the corresponding screw rods, driving the base to move downward in the corresponding cylindrical experimental tube. The cleaning and disinfection liquid in the cleaning and disinfection liquid tank is pumped into the liquid inlet at high pressure, and the cleaning and disinfection liquid is sprayed along the liquid inlet - liquid collection chamber - a circle of inlet holes - shaft channel - a circle of outlet holes - connecting pipe - liquid spray oblique outlet hole and liquid spray bottom outlet hole. The liquid spray oblique outlet hole sprays obliquely at high pressure. The cleaning and disinfecting liquid generates a tangential force, causing the rotatable shaft to rotate, thereby driving the rotatable spraying ring to rotate, so that the rotatable spraying ring moves downward while rotating, and the cleaning and disinfecting liquid sprayed from the liquid spray oblique outlet hole is rotationally sprayed toward the inner wall of the corresponding cylindrical experimental cylinder to clean and disinfect the inner wall, and the cleaning and disinfecting liquid sprayed from the liquid spray bottom outlet hole is rotationally sprayed toward the bottom of the corresponding cylindrical experimental cylinder to clean and disinfect the inner bottom, thereby realizing the function of cleaning and disinfecting the corresponding cylindrical experimental cylinder, and the cleaning and disinfecting liquid in the corresponding cylindrical experimental cylinder is discharged through the corresponding drain pipe, and after the cleaning and disinfection time reaches the set cleaning and disinfection time, the corresponding drive motor is controlled to drive the base to reset and the corresponding drain electric valve to close.

[0024] The positive progress effect of the present invention is:

[0025] The present invention can realize the whole process of forced swimming experiment of small animals in a comprehensive, intelligent and automated control mode, specifically realizes the function of automatically adding water to adjust the water depth, automatically heating water to adjust the water temperature, automatically surging water to adjust the water flow, realizes the function of automatic video monitoring of the forced swimming situation of the experimental small animals, realizes the function of automatically catching the experimental small animals when it is analyzed that the experimental small animals are in an exhausted state, and realizes the function of automatically catching the experimental small animals when the experimental small animals are caught and stay on the water surface for a period of time. When the experimental small animals have rested, they are put into the water again to continue the forced swimming experiment, realizes the function of automatically catching the experimental small animals when the experimental time is reached, realizes the function of automatically draining water, and realizes the function of automatic cleaning and disinfection.

[0026] In realizing the function of automatically catching experimental small animals, the present invention cleverly designs an automatic catching structure. When there is no need to catch experimental small animals, the automatic catching structure is designed to make the animal catching net in a retracted state. When there is a need to catch experimental small animals, the automatic catching structure is designed to make the animal catching net in an unfolded state to catch the experimental small animals. Compared with the existing structure that always keeps the animal catching net in an unfolded state, this structure has a more clever structural design and reduces the space occupied by the animal catching net when not in use.

[0027] In realizing the function of automatic cleaning and disinfection, the present invention cleverly designs an automatic cleaning and disinfection structure. Compared with the cleaning and disinfection structure of the prior art, this automatic cleaning and disinfection structure can move downward while rotatingly spraying cleaning and disinfection liquid to clean and disinfect the circumferential inner wall and bottom of the cylindrical laboratory tube, which can clean and disinfect more thoroughly.

[0028] The present invention can accurately record the water depth, water temperature and water flow of each cylindrical experimental tube in the animal experiment, the start time of the animal experiment, the end time of the animal experiment, the time point when each animal is picked up, the number of swimming behaviors and the corresponding duration between the time points when the previous and subsequent animals are picked up, the number of climbing behaviors and the corresponding duration, and the number of stationary behaviors and the corresponding duration during the entire animal experiment, thereby recording some important experimental information during the animal experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the box structure of the experimental small animal forced swimming system according to a preferred embodiment of the present invention.

[0030] Figure 2 and Figure 3 Schematic diagrams of the internal and external structures of a cylindrical test tube according to a preferred embodiment of the present invention from different angles.

[0031] Figure 4 Schematic diagram of the structure of the electric push rod and L-shaped connecting rod in a preferred embodiment of the present invention.

[0032] Figure 5 This is a schematic structural diagram of a limit sensor and a small counterweight block according to a preferred embodiment of the present invention.

[0033] Figure 6 Schematic diagram of the structure of the hinge of the L-shaped connecting rod in a preferred embodiment of the present invention.

[0034] Figure 7 and Figure 8 Schematic diagrams from different angles of the upper structure of the cleaning and disinfection structure of a preferred embodiment of the present invention.

[0035] Figure 9 This is a schematic structural diagram of the lower structure of the cleaning and disinfection structure of a preferred embodiment of the present invention.

[0036] Figure 10 This is a schematic structural diagram of the cleaning and disinfection structure of a preferred embodiment of the present invention.

[0037] Figure 11 This is a cross-sectional view of the cleaning and disinfection structure of a preferred embodiment of the present invention. DETAILED DESCRIPTION

[0038] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0039] like Figure 1 As shown, this embodiment provides an experimental small animal forced swimming system, which includes four support columns 1 located at four corners, and a box body 2 is fixed on the top support of the four support columns 1. The box body 2 is provided with four cylindrical placement cavities, and the four cylindrical placement cavities are in the shape of a field. Each cylindrical placement cavity is fixed with a heat-conductive cylindrical experimental tube 3 with open top and bottom. Each cylindrical experimental tube 3 is correspondingly provided with a lower computer, and a control box 4 is fixed on the front of the box body 2. The four lower computers are integrated in the control box 4. A touch screen 5 is embedded in the control box 4, and each lower computer is communicatively connected to the upper computer.

[0040] like Figure 1 and Figure 2 As shown, each cylindrical test tube 3 is provided with a corresponding water inlet pipe 6. One end of each water inlet pipe 6 passes through the box body 2 and the corresponding cylindrical test tube 3 and is placed on the upper portion of the corresponding cylindrical test tube 3. The other end of each water inlet pipe 6 is placed outside the box body 2 for connecting to an external water source. Each water inlet pipe 6 is provided with a water inlet electric valve 7, which is also placed outside the box body 2. Multiple liquid level sensors 8 are evenly spaced vertically from bottom to top on the inner wall of each cylindrical test tube 3, forming a row of liquid level sensors. Utilizing this automatic water-adding structure, an automatic water-adding function can be achieved.

[0041] like Figure 2 As shown, the outer wall of each cylindrical test tube 3 is wound with a spiral electric heating wire 9 from bottom to top, and the inner bottom of each cylindrical test tube 3 is fixed with a temperature sensor 10. With this automatic heating structure, the automatic water heating function can be realized.

[0042] like Figure 2 As shown, electric propellers 11 are respectively fixed on opposite sides of the inner bottom of each cylindrical test tube 3. By utilizing this automatic water flow surging structure, the automatic water flow surging function can be realized.

[0043] like Figure 2 As shown, a camera 12 is fixed to the inner top of each cylindrical test tube 3, and the camera 12 faces the corresponding cylindrical test tube 3. With this camera 12, an automatic monitoring function can be realized.

[0044] The inner wall of each cylindrical experimental tube 3 is roughened from the middle to a position a certain distance from the top (not shown in the figure) to facilitate climbing of the experimental animals.

[0045] like Figure 2-5 As shown, an electric push rod 13 is fixed to the outside of the bottom of the box body 2 and directly below the middle position of each cylindrical experimental tube 3. A sleeve 14 is fixed to the middle position of the inner bottom of each cylindrical experimental tube 3. The push rod end of each electric push rod 13 passes through the bottom of the box body 2 and is placed in the corresponding sleeve 14. The push rod of each electric push rod 13 is sealed with the penetration position of the bottom of the box body 2, so that when the push rod of the electric push rod 13 moves up and down, the water in the cylindrical experimental tube 3 will not flow out of the box body 2 along the penetration position. A round pedestal seat 15 is fixed to the top of the push rod end of each electric push rod 13. Four L-shaped connecting rods 16 are hinged to the surface of each round pedestal seat 15 along the circumferential direction. These A limit sensor 17 is fixed to the bottom of the crossbar of one of the L-shaped connecting rods 16. Small counterweights 18 are fixed to the bottom of the crossbar ends of these L-shaped connecting rods 16. Animal catching nets (not shown in the figure, refer to existing animal catching nets) are fixed to the tops of these L-shaped connecting rods 16. When these L-shaped connecting rods 16 are in the initial state, the vertical rods of these L-shaped connecting rods 16 are placed in the corresponding sleeves 14, and the crossbars of these L-shaped connecting rods 16 are placed on the top outer edge of the sleeves 14, and the corresponding animal catching nets are in a contracted state. When these L-shaped connecting rods 16 are in the expanded state, the corresponding animal catching nets are in an expanded state, and the expanded animal catching nets cover the horizontal surface of the corresponding cylindrical experimental tube 3. Utilizing this automatic catching structure, an automatic animal catching function can be realized.

[0046] Among them, Figure 6 As shown, the specific implementation method of each pedestal seat 15 is that four L-shaped connecting rods 16 are hingedly connected to the surface along the circumferential direction: four mounting seats 19 with U-shaped transverse cross-sections and U-shaped openings facing outward are fixed on the surface of each pedestal seat 15 along the circumferential direction, a fixed shaft 20 is fixed between the opposite sides of the U-shape of each mounting seat 19, a bearing 21 is fixedly mounted at the middle position of each fixed shaft 20, and each bearing 21 is fixedly connected to an L-shaped connecting rod 16, and the U-shaped bottom of each mounting seat 19 plays a limiting role, so that the L-shaped connecting rod 16 is in a vertical state when all are placed in the sleeve 14, and the rotatable angle of each L-shaped connecting rod 16 is 90 degrees. The vertical part of each L-shaped connecting rod 16 can be rotated clockwise from a vertical state to a horizontal state or rotated counterclockwise from a horizontal state to a vertical state.

[0047] like Figure 2 As shown, the top of the inner wall of each cylindrical placement cavity and the top of the outer wall of the corresponding cylindrical test cylinder 3 are sealed by a circular sealing plate 22 to prevent water in the cylindrical test cylinder 3 from entering the gap between the cylindrical placement cavity and the corresponding cylindrical test cylinder 3.

[0048] like Figure 3 As shown, a drain pipe 23 is inserted and fixed at the bottom of the box body 2 and at the bottom of each cylindrical test tube 3, and a drain electric valve 24 is provided on each drain pipe 23 and outside the box body 2. With this automatic drain structure, the automatic drain function can be realized.

[0049] like Figure 2 、 Figure 3 、 Figure 7-11 As shown, two driving motors 25 are fixed to the outside of the bottom of the box body 2 and directly below each cylindrical experimental tube 3. The driving ends of the two driving motors 25 pass through the bottom of the corresponding cylindrical experimental tube 3 and are fixed with screw rods 26. The two screw rods 26 are respectively placed on opposite sides of the inner wall of the corresponding cylindrical experimental tube 3. A nut 27 is sleeved on each screw rod 26, and a base 28 with a center hole is fixed between the two nuts 27. The base 28 includes an upper fixing ring 281, a diamond base body 282 with a center hole and a lower fixing ring 283 stacked in sequence from top to bottom. The upper fixing ring 281, the diamond base body 282 and the lower fixing ring 283 are coaxially arranged and integrally formed.

[0050] A bearing mounting seat 29 coaxially arranged with the center hole is provided in the base 28, and a bearing 30 is installed in the bearing mounting seat 29. A bearing ring cover 32 is provided at the bottom of the base 28 through a sealing ring 31. The bearing ring cover 32 is located directly below the bearing 30. The bearing 30 is sealed in the base 28 by the bearing ring cover 32. The base 28 and the bearing ring cover 32 are fixed by bolts (not shown in the figure).

[0051] Liquid inlets 33 connected to an external cleaning and disinfecting liquid tank are respectively provided on the base 28 and on both sides of the bearing 30 along the vertical direction. Each liquid inlet 33 is penetrated by a corresponding upper fixing ring 282, a diamond-shaped base body 282 and a lower fixing ring 283.

[0052] The bearing 30 is connected to a rotatable shaft 34 through an axis, and the rotatable shaft 34 also passes through the center hole of the base 28. A circle of inlet holes 35 and a circle of outlet holes 36 are vertically spaced apart on the rotatable shaft 34 and below the bearing ring cover 32. The circle of inlet holes 35 are inlet holes evenly spaced along the circumferential direction, and the circle of outlet holes 36 are outlet holes evenly spaced along the circumferential direction. The circle of inlet holes 35 is located above the circle of outlet holes 36, wherein the inlet holes and the outlet holes correspond one to one. An axial channel 37 connecting the inlet holes and the corresponding outlet holes is opened in the rotatable shaft 34, and a blocking cover 38 is fixed to the bottom of the rotatable shaft 34.

[0053] A liquid collecting tray 39 is sleeved on the rotatable shaft 34, and the top of the liquid collecting tray 39 is screwed to the bottom of the base 28 (i.e., the bottom end of the lower fixing ring 283). The liquid collecting cavity 40 of the liquid collecting tray 39 corresponds to the circle of inlet holes 35, so that the cleaning and disinfecting liquid entering the liquid inlet 33 first flows into the liquid collecting cavity 40, and then flows into the shaft channel 37 through the circle of inlet holes 35. A rotatable spray ring 41 is sleeved and fixed on the rotatable shaft 34, which can rotatably spray. Ring body 41 is located between liquid collection tray 39 and plug cover 38. A connecting pipe 42 is provided on the inner wall of rotatable spray ring body 41 along its diameter, corresponding to each of the outlet holes 36. The outer periphery of rotatable spray ring body 41 is provided with oblique liquid spray outlet holes 43 that communicate with corresponding connecting pipes 42. The bottom end of rotatable spray ring body 41 is provided with multiple liquid spray bottom outlet holes 44 that communicate with corresponding connecting pipes 42. This automatic cleaning and disinfection structure can achieve automatic cleaning and disinfection functions.

[0054] Before starting the forced swimming experiment on small experimental animals such as mice, it is necessary to first inject water into each cylindrical experimental tube 3 to adjust the temperature and flow of the water in each cylindrical experimental tube 3 .

[0055] Automatic water addition and depth adjustment function: The upper computer is used to send a water depth control instruction containing a set water depth value to the corresponding lower computer. Upon receiving this water depth control instruction, the corresponding lower computer is used to activate the corresponding row of liquid level sensors 8. Based on the corresponding row of liquid level sensors 8, it obtains the current water depth value in the corresponding cylindrical test tube 3 and determines whether the current water depth value has reached the corresponding set water depth value. If the current water depth value reaches the corresponding set water depth value, it sends a water depth control complete instruction to the upper computer. If the current water depth value reaches the corresponding set water depth value, it controls the corresponding water inlet electric valve 7 to open, and adds water to the corresponding cylindrical test tube 3 through the water source connected to the corresponding water inlet pipe 6 until the current water depth value reaches the corresponding set water depth value, and controls the corresponding water inlet electric valve 7 to close. Through this operation, the water in each cylindrical test tube 3 is added to the corresponding target water depth.

[0056] Automatic water temperature rise and adjustment function: After receiving the instruction indicating that the water depth has been controlled, the upper computer is used to send a water temperature control instruction containing the set water temperature value to the corresponding lower computer. The corresponding lower computer is used to start the corresponding temperature sensor 10 after receiving the water temperature control instruction. Based on the current water temperature value in the corresponding cylindrical test tube 3 obtained by the corresponding temperature sensor 10, it is judged whether the current water temperature value reaches the corresponding set water temperature value. If the current water temperature value reaches the corresponding set water temperature value, it sends an instruction indicating that the water temperature has been controlled to the upper computer. If the current water temperature value reaches the corresponding set water temperature value, it controls the corresponding spiral electric heating wire 9 to start, heating the water in the corresponding cylindrical test tube 3, and pausing the spiral electric heating wire 9 until the current water temperature reaches the corresponding set water temperature value. Through this operation, the water temperature in each cylindrical test tube 3 is adjusted to the corresponding target water temperature.

[0057] Automatic water flow regulation: Upon receiving a command indicating that water temperature control has been completed, the host computer issues a water flow control command containing the set water flow intensity to the corresponding slave computer. Upon receiving this water flow control command, the corresponding slave computer activates the electric propeller 11 in the corresponding cylindrical test tube 3 and controls the corresponding electric propeller 11 to rotate at a speed that matches the set water flow intensity. This operation adjusts the water flow in each cylindrical test tube 3 to the corresponding target water flow.

[0058] The water depth, water temperature and water flow in each cylindrical experimental tube 3 have been adjusted according to the target, and the experimental conditions for the forced swimming of the experimental animals have been prepared. Now, the experimental animals are placed in the water to conduct the forced swimming experiment.

[0059] The upper computer is used to send a video monitoring instruction to the corresponding lower computer, and record the start time of the animal experiment at this time. The corresponding lower computer is used to start the corresponding camera 12 after receiving the video monitoring instruction to shoot the forced swimming situation of the experimental small animal in the corresponding cylindrical experimental tube 3 and transmit it to the upper computer. The upper computer is used to analyze the current experimental behavior of the experimental small animal based on the forced swimming situation of the experimental small animal using image processing technology. The experimental behavior includes swimming behavior, climbing behavior, stationary behavior and exhaustion behavior (such as the phenomenon of experimental small animals, no experimental small animals, experimental small animals, and no experimental small animals appearing in several consecutive images, or no experimental small animals appearing in several images). When it is judged that the current experimental behavior is exhaustion behavior, an animal fishing instruction is sent to the corresponding lower computer, and the time point when the animal is fished out at this time is recorded, and the number of swimming behaviors and the corresponding duration, the number of climbing behaviors and the corresponding duration, and the number of stationary behaviors and the corresponding duration between the time points when the animals are fished out. The corresponding duration, the corresponding lower computer is used to start the corresponding electric push rod 13 after receiving the corresponding animal fishing instruction, control the corresponding electric push rod 13 to push the L-shaped connecting rod 16 thereon to move upward, and these L-shaped connecting rods 16 gradually expose the corresponding sleeves 14, and these L-shaped connecting rods 16 are unfolded under the action of the corresponding small counterweight blocks 18, and the corresponding animal fishing net is unfolded to catch the experimental small animals. The animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, controls the electric push rod to pause, and judges whether the time the experimental small animals stay above the water surface reaches the set stay time. When it is, start the corresponding electric push rod 13, control the corresponding electric push rod 13 to reset, and when the limit sensor 17 touches the corresponding sleeve 14, the corresponding lower computer receives the limit information from the limit sensor 17, indicating that the corresponding electric push rod 13 has been reset, pauses the corresponding electric push rod 13, and puts the experimental small animals into the water again to continue to be forced to swim, and repeats the shooting, catching and putting them into the water operations.

[0060] The upper computer is used to record the end time of the animal experiment when the experiment time reaches the set experiment time, and send an animal fishing instruction to the corresponding lower computer. The corresponding lower computer is used to start the corresponding electric push rod 13 after receiving the corresponding animal fishing instruction, control the corresponding electric push rod 13 to push the L-shaped connecting rod 16 thereon to move upward, and the corresponding animal fishing net is unfolded to catch the experimental small animals. The animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, controls the electric push rod 13 to pause, and an experiment ends.

[0061] At this point, an experiment is completed. During this experiment, the touch screen 5 can be divided into 4 display areas, each display area can be used to display information such as the water depth, water temperature and water flow in the corresponding cylindrical experimental tube 3, the start time of this animal experiment, the end time of this animal experiment, the time point when each animal is fished out, the number of swimming behaviors and the corresponding duration between the time points when the previous and subsequent animals are fished out, the number of climbing behaviors and the corresponding duration, and the number of stationary behaviors and the corresponding duration.

[0062] Automatic water discharge function: The upper computer is used to send a water discharge control instruction to the corresponding lower computer after the end of an experiment. The corresponding lower computer is used to start the corresponding water discharge electric valve 24 after receiving the water discharge control instruction, so that the water in the corresponding cylindrical experimental tube 3 is discharged through the corresponding water discharge pipe 23, and control the corresponding water discharge electric valve 24 to close after the water discharge time reaches the set water discharge time.

[0063] Automatic cleaning and disinfection function: The upper computer is used to send a cleaning and disinfection control instruction to the corresponding lower computer after an experiment is completed. The corresponding lower computer is used to control the corresponding two drive motors 25 to start and the corresponding water discharge electric valve 24 to open after receiving the cleaning and disinfection control instruction. The two drive motors 25 drive the corresponding screw rods 26 to rotate, and the two nuts 27 gradually move downward along the corresponding screw rods 26, driving the base 28 to move downward in the corresponding cylindrical experimental tube 3. The liquid inlet 33 is externally connected to the cleaning and disinfection liquid tank. The cleaning and disinfection liquid in the cleaning and disinfection liquid tank is pumped into the liquid inlet 33 under high pressure. The cleaning and disinfection liquid flows into the liquid collection chamber 40 along the liquid inlet 33. The cleaning and disinfection liquid in the liquid collection chamber 40 flows into a circle of inlet holes 35. The cleaning and disinfection liquid in a circle of inlet holes 35 flows into a circle of outlet holes 36 along the corresponding axial channels 37, and then flows into the rotatable spray ring 41 along the connecting pipe 42, and then flows along the liquid spray oblique outlet hole 43 and the liquid spray bottom outlet hole 44. Spraying, since the liquid spraying oblique outlet hole 43 sprays the cleaning and disinfecting liquid at an oblique high pressure, it can generate a tangential force, so that the rotatable spraying ring body 41 has a rotational force, and since the rotatable spraying ring body 41 is fixed on the rotatable shaft 34, the rotational force causes the rotatable shaft 34 to rotate, thereby driving the rotatable spraying ring body 41 to rotate, so that the rotatable spraying ring body 41 moves downward while rotating, and the cleaning and disinfecting liquid sprayed from the liquid spraying oblique outlet hole 43 is rotated and sprayed toward the inner wall of the corresponding cylindrical experimental tube 3 to clean and disinfect the inner wall. The cleaning and disinfecting liquid sprayed from the liquid spraying bottom outlet hole 44 is rotated and sprayed toward the inner bottom of the corresponding cylindrical experimental tube 3 to clean and disinfect the inner bottom, thereby realizing the function of cleaning and disinfecting the corresponding cylindrical experimental tube 3. The cleaning and disinfecting liquid in the corresponding cylindrical experimental tube 3 is discharged through the corresponding drain pipe 23, and after the cleaning and disinfection time reaches the set cleaning and disinfection time, the corresponding drive motor 25 is controlled to drive the base to reset and the corresponding drain electric valve 24 to close.

[0064] In this embodiment, the flow path of the cleaning and disinfecting liquid is: liquid inlet 33 - liquid collection chamber 40 - a circle of inlet holes 35 - axial channel 37 - a circle of outlet holes 36 - connecting pipe 42 - liquid spray oblique outlet hole 43 and liquid spray bottom outlet hole 44.

[0065] Although specific embodiments of the present invention have been described above, those skilled in the art will appreciate that these are merely illustrative and that the scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, and such changes and modifications are intended to fall within the scope of the present invention.

Claims

1. A forced swimming system for experimental small animals, characterized in that: It includes four support columns located at four corners, and a box is fixed on the top support of the four support columns. The box has multiple cylindrical placement cavities, and each cylindrical placement cavity is fixed with a thermal conductive cylindrical experimental cylinder with open top and bottom. Each cylindrical experimental cylinder is correspondingly provided with a lower computer, and each lower computer is communicatively connected to the upper computer. Each of the cylindrical test cylinders is provided with a corresponding water inlet pipe, one end of each of the water inlet pipes passes through the box and the corresponding cylindrical test cylinder and is placed on the upper part of the corresponding cylindrical test cylinder, and the other end of each of the water inlet pipes is placed outside the box, each of the water inlet pipes is provided with a water inlet electric valve and the water inlet electric valve is placed outside the box, and the inner wall of each of the cylindrical test cylinders is evenly spaced from bottom to top along the vertical direction. A plurality of liquid level sensors are arranged to form a row of liquid level sensors; The outer wall of each cylindrical experimental tube is wound with a spiral electric heating wire from bottom to top, and the inner bottom of each cylindrical experimental tube is fixed with a temperature sensor; An electric propeller is fixed on opposite sides of the inner bottom of each cylindrical test tube; A camera is fixed to the inner top of each cylindrical test tube, and the camera faces the corresponding cylindrical test tube; The inner wall of each cylindrical experimental tube is roughened from the middle to a position a certain distance from the top to facilitate climbing by experimental animals; An electric push rod is fixed to the outside of the bottom of the box and directly below the middle position of each cylindrical experimental tube. A sleeve is fixed to the middle position of the inner bottom of each cylindrical experimental tube. The push rod end of each electric push rod passes through the bottom of the box and is placed in the corresponding sleeve. A round pedestal seat is fixed to the top of the push rod end of each electric push rod. A plurality of L-shaped connecting rods are hinged on the surface of each round pedestal seat along the circumferential direction. The bottom of the cross bar of one of the L-shaped connecting rods is fixed with a limited Position sensors, small counterweights are fixed to the bottoms of the crossbar ends of the L-shaped connecting rods, and animal fishing nets are fixed to the tops of the L-shaped connecting rods. When the L-shaped connecting rods are in an initial state, the vertical rods of the L-shaped connecting rods are placed in corresponding sleeves, and the crossbars of the L-shaped connecting rods are placed on the outer edges of the tops of the sleeves, corresponding to the animal fishing nets being in a retracted state. When the L-shaped connecting rods are in an expanded state, the corresponding animal fishing nets are in an expanded state, and the expanded animal fishing nets cover the lateral surfaces of the corresponding cylindrical experimental tubes; The upper computer is used to send a water depth control instruction containing a set water depth value to the corresponding lower computer, and the corresponding lower computer is used to start the corresponding row of liquid level sensors after receiving the water depth control instruction, obtain the current water depth value in the corresponding cylindrical test cylinder based on the corresponding row of liquid level sensors, judge whether the current water depth value reaches the corresponding set water depth value, and send an instruction to the upper computer that the water depth has been controlled if the current water depth value reaches the corresponding set water depth value. If the current water depth value reaches the corresponding set water depth value, the upper computer is controlled to open the corresponding water inlet electric valve, and water is added to the corresponding cylindrical test cylinder through the corresponding water inlet pipe until the current water depth value reaches the corresponding set water depth value and the corresponding water inlet electric valve is controlled to close. The upper computer is used to, after receiving the instruction that the water depth has been controlled, send a water temperature control instruction containing a set water temperature value to the corresponding lower computer, and the corresponding lower computer is used to start the corresponding temperature sensor after receiving the water temperature control instruction, obtain the current water temperature value in the corresponding cylindrical experimental cylinder based on the corresponding temperature sensor, judge whether the current water temperature value reaches the corresponding set water temperature value, and send the instruction that the water temperature has been controlled to the upper computer when the answer is yes, and control the corresponding spiral electric heating wire to start when the answer is no, so as to heat the water in the corresponding cylindrical experimental cylinder until the current water temperature value reaches the corresponding set water temperature value, and then control the spiral electric heating wire to pause; The upper computer is used to send a water flow surge control instruction containing a set water flow intensity to the corresponding lower computer after receiving the instruction that the water temperature has been controlled. The corresponding lower computer is used to start the electric propeller in the corresponding cylindrical test tube after receiving the water flow surge control instruction and control the corresponding electric propeller to rotate at a speed matching the set water flow intensity; The upper computer is used to send a video monitoring instruction to the corresponding lower computer, and record the start time of the animal experiment at this time. The corresponding lower computer is used to start the corresponding camera after receiving the video monitoring instruction to shoot the forced swimming situation of the experimental small animal in the corresponding cylindrical experimental tube and transmit it to the upper computer. The upper computer is used to analyze the current experimental behavior of the experimental small animal based on the forced swimming situation of the experimental small animal using image processing technology. The experimental behavior includes swimming behavior, climbing behavior, stationary behavior and exhaustion behavior. When it is judged that the current experimental behavior is exhaustion behavior, an animal fishing instruction is sent to the corresponding lower computer, and the time point when the animal is fished out at this time is recorded, and the number of swimming behaviors and the corresponding duration, the number of climbing behaviors and the corresponding duration, and the number of stationary behaviors between the time points when the animal is fished out are recorded. and the corresponding duration, the corresponding lower computer is used to start the corresponding electric push rod after receiving the corresponding animal fishing instruction, control the corresponding electric push rod to push the L-shaped connecting rod thereon to move upward, the L-shaped connecting rods gradually expose the corresponding sleeves, and the L-shaped connecting rods are unfolded under the action of the corresponding small counterweight blocks, and the corresponding animal fishing net is unfolded to catch the experimental small animals. The animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, controls the electric push rod to pause, and judges whether the time the experimental small animals stay above the water surface reaches the set stay time. When the time is yes, starts the corresponding electric push rod, controls the corresponding electric push rod to reset, and pauses the corresponding electric push rod when receiving the limit information from the limit sensor, and puts the experimental small animals into the water again and continues to force them to swim, and repeats the shooting, scooping up and putting them into the water operations; The upper computer is used to record the end time of the animal experiment when the experiment time reaches the set experiment time, and send an animal fishing instruction to the corresponding lower computer. The corresponding lower computer is used to start the corresponding electric push rod after receiving the corresponding animal fishing instruction, control the corresponding electric push rod to push the L-shaped connecting rod thereon to move upward, and the corresponding animal fishing net is unfolded to catch the experimental small animals. After the animal fishing net catches the experimental small animals and places the experimental small animals above the water surface, the electric push rod is controlled to pause, and an experiment ends.

2. The experimental small animal forced swimming system according to claim 1, characterized in that: A drain pipe is fixedly installed at the bottom of the box body and at the bottom of each cylindrical test tube, and a drain electric valve is installed on each drain pipe and outside the box body; The upper computer is used to send a water discharge control instruction to the corresponding lower computer after the end of an experiment. The corresponding lower computer is used to start the corresponding water discharge electric valve after receiving the water discharge control instruction, so that the water in the corresponding cylindrical experimental tube is discharged through the corresponding water discharge pipe, and control the corresponding water discharge electric valve to close after the water discharge time reaches the set water discharge time.

3. The experimental small animal forced swimming system according to claim 2, characterized in that: Two driving motors are fixed to the outside of the bottom of the box and directly below each cylindrical experimental cylinder. The driving ends of the two driving motors pass through the bottom of the corresponding cylindrical experimental cylinder and are fixed with screws. The two screws are respectively placed on opposite sides of the inner wall of the corresponding cylindrical experimental cylinder. A nut is sleeved on each of the screws. A base with a center hole is fixed between the two nuts. A bearing mounting seat coaxially arranged with the center hole is provided in the base. A bearing is installed in the bearing mounting seat. A bearing ring cover is provided at the bottom of the base through a sealing ring cover. The bearing ring cover is located directly below the bearing. The bearing is sealed in the base by the bearing ring cover, and the base and the bearing ring cover are fixed by bolts. The base is provided with liquid inlets for connecting an external cleaning and disinfecting liquid tank on both sides of the bearing in the vertical direction. The bearing is provided with a rotatable shaft through the shaft. A circle of inlet holes and a circle of outlet holes are vertically spaced apart on the rotatable shaft and below the bearing ring cover. The circle of inlet holes is inlet holes evenly spaced apart along the circumferential direction, and the circle of outlet holes is outlet holes evenly spaced apart along the circumferential direction. The circle of inlet holes is located above the circle of outlet holes, and the inlet holes and outlet holes correspond to each other one by one. An on-axis channel connecting the inlet hole and the corresponding outlet hole is provided in the rotatable shaft, and a blocking cover is fixed to the bottom of the rotatable shaft. A liquid collecting tray is sleeved on the rotatable shaft, and the top of the liquid collecting tray is screwed to the bottom of the base, and the liquid collecting cavity of the liquid collecting tray corresponds to a circle of inlet holes, so that the cleaning and disinfecting liquid entering the liquid inlet first flows into the liquid collecting cavity and then flows into the channel on the shaft through a circle of inlet holes. A rotatable spraying ring body is sleeved and fixed on the rotatable shaft, and the rotatable spraying ring body is located between the liquid collecting tray and the blocking cover. A connecting pipe corresponding to each outlet hole in a circle of outlet holes is opened on the inner wall of the rotatable spraying ring body along the diameter direction of the rotatable spraying ring body, and the outer wall periphery of the rotatable spraying ring body is opened with liquid spraying oblique outlet holes connected with the corresponding connecting pipes, and the bottom end of the rotatable spraying ring body is opened with a plurality of liquid spraying bottom outlet holes connected with the corresponding connecting pipes; The upper computer is used to send a cleaning and disinfection control instruction to the corresponding lower computer after an experiment is completed. The corresponding lower computer is used to control the corresponding two drive motors to start and the corresponding water discharge electric valve to open after receiving the cleaning and disinfection control instruction. The two drive motors drive the corresponding screw rods to rotate, and the two nuts gradually move downward along the corresponding screw rods, driving the base to move downward in the corresponding cylindrical experimental tube. The cleaning and disinfection liquid in the cleaning and disinfection liquid tank is pumped into the liquid inlet at high pressure, and the cleaning and disinfection liquid is sprayed along the liquid inlet - liquid collection chamber - a circle of inlet holes - shaft channel - a circle of outlet holes - connecting pipe - liquid spray oblique outlet hole and liquid spray bottom outlet hole. The liquid spray oblique outlet hole sprays obliquely at high pressure. The cleaning and disinfecting liquid generates a tangential force, causing the rotatable shaft to rotate, thereby driving the rotatable spraying ring to rotate, so that the rotatable spraying ring moves downward while rotating, and the cleaning and disinfecting liquid sprayed from the liquid spray oblique outlet hole is rotationally sprayed toward the inner wall of the corresponding cylindrical experimental cylinder to clean and disinfect the inner wall, and the cleaning and disinfecting liquid sprayed from the liquid spray bottom outlet hole is rotationally sprayed toward the bottom of the corresponding cylindrical experimental cylinder to clean and disinfect the inner bottom, thereby realizing the function of cleaning and disinfecting the corresponding cylindrical experimental cylinder, and the cleaning and disinfecting liquid in the corresponding cylindrical experimental cylinder is discharged through the corresponding drain pipe, and after the cleaning and disinfection time reaches the set cleaning and disinfection time, the corresponding drive motor is controlled to drive the base to reset and the corresponding drain electric valve to close.

4. The experimental small animal forced swimming system according to claim 3, characterized in that: The base includes an upper fixing ring, a diamond-shaped base body with a central hole, and a lower fixing ring stacked in sequence from top to bottom, wherein the upper fixing ring, the diamond-shaped base body, and the lower fixing ring are coaxially arranged and integrally formed; Each of the liquid inlets passes through a corresponding upper fixing ring, a diamond-shaped base body and a lower fixing ring, and the top of the liquid collecting plate is screwed to the bottom of the lower fixing ring.

5. The experimental small animal forced swimming system according to claim 1, characterized in that: The surface of each of the truncated pedestals is fixed with four mounting seats with a U-shaped transverse cross-section and the U-shaped opening facing outward along the circumferential direction. A fixed shaft is fixed between the opposite sides of the U-shape of each mounting seat. A bearing is fixed at the middle position of each fixed shaft, and each bearing is fixedly connected to an L-shaped connecting rod.

6. The experimental small animal forced swimming system according to claim 1, characterized in that: A control box is fixed on the front of the box body, the lower computers are integrated in the control box, and a display screen is embedded in the control box.

7. The experimental small animal forced swimming system according to claim 6, characterized in that: The box body is provided with four cylindrical placement cavities, and the four cylindrical placement cavities are in a field shape. The four lower computers are all integrated in the control box, and the display screen is a touch display screen.

8. The experimental small animal forced swimming system according to claim 1, characterized in that: The top of the inner wall of each cylindrical placement cavity is sealed with the top of the outer wall of the corresponding cylindrical test tube through a circular sealing plate to prevent water in the cylindrical test tube from entering the gap between the cylindrical placement cavity and the corresponding cylindrical test tube.

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