An agent for controlling pine wood nematode, its method and application

Abstract

This invention relates to the field of chemical control technology, and more particularly to a pesticide, method, and application for controlling pine wilt disease. The pesticide for controlling pine wilt disease of this invention comprises the following components: bromocyanamide, chlorantraniliprole, abamectin, bark penetrant, and water. The pesticide is injected into the base of the pine tree trunk or roots using a perforation injection method. This method controls both vector insects and pine wilt disease, overcomes the problem of resin blockage, and allows the pesticide to act quickly and effectively. The operation is simple, saves labor, and has low control costs.

Description

Technical Field

[0001] This invention relates to the field of chemical control technology, and in particular to an agent, method and application for controlling pine wilt disease. Background Technology

[0002] The life cycle of the pine wood nematode consists of a reproductive cycle and a dispersal cycle. The entire reproductive cycle takes place within the pine tree. When a vector insect carrying the nematode feeds on a healthy pine branch, the nematode enters the tree and begins its reproductive cycle, repeatedly producing eggs, larvae, and adults. Second-instar larvae, encountering adverse environments, transform into dispersal third-instar larvae, which gather in the pupal chamber. They are resistant to dryness and low temperatures. After molting, they gradually become persistent (dormant) fourth-instar larvae. These persistent fourth-instar larvae are particularly resistant to dryness and are well-suited for vector insect dispersal. At 25°C, the pine wood nematode has one generation every 5 days; one pair of nematodes can reproduce 200,000 pine wood nematodes within 20 days.

[0003] Pine wilt disease (also known as pine wilt), caused by the pine nematode, is called the cancer of pine trees. It is a devastating disease characterized by multiple transmission routes, hidden infection sites, rapid onset, long incubation period, and difficulty in control. The most prominent external symptoms after the pine nematode invades the tree are: first, the needles lose water and turn pale green, then brown, and finally the entire tree dies, with the needles turning entirely reddish-yellow. Its development can be divided into four stages: ① Normal appearance, reduced or stopped resin secretion, and decreased transpiration; ② Needles begin to change color, resin secretion stops, and traces of longhorn beetle or other beetle infestation and egg-laying can usually be observed; ③ Most needles turn yellowish-brown and wilt, and the debris from longhorn beetles or other beetles is usually visible; ④ All needles turn yellowish-brown, the diseased tree dries up and dies, but the needles do not fall off. At this stage, various pests usually inhabit the tree.

[0004] The control of pine wilt disease mainly employs quarantine control, chemical control, culling of infected trees, and silvicultural measures. According to the "Technical Program for the Control of Pine Wilt Disease (2021 Edition)," my country's control strategy for pine wilt disease focuses on culling diseased (dying, dead) pine trees, fundamentally controlling infected trees, and supplementing with insect vector control and injection of pesticides. Currently, pine wilt disease control both domestically and internationally is divided into two categories: controlling vector insects and controlling the pine wilt nematode itself. The main methods for controlling vector insects (pine sawyer beetle) include parasitism by parasitic wasps, application of Beauveria bassiana, and trapping, but these methods are not very effective, especially since the application of Beauveria bassiana and other fungicides has a significant impact on other insects in the forest, such as bees. The main method for controlling pine wilt disease is through injection via drilling. The main pesticides used are abamectin, thiamethoxam, imidacloprid, cypermethrin, and emamectin benzoate, which have a certain control effect on pine wilt disease and are widely used in scenic areas. However, when the pesticide is injected into the tree trunk, it is easily blocked by the pine resin secreted by the pine tree, which hinders the conduction of the pesticide in the tree and results in poor control effect.

[0005] The nationwide control measures for pine wilt disease involve felling infected trees and then processing and utilizing or burning the felled trees on-site. Although this can locally control the spread of pine wilt disease, the control effect is poor because it does not block the longhorn beetle from carrying pine wilt nematodes, and it can lead to the continuous spread of the epidemic.

[0006] Therefore, in order to effectively control pine wilt disease, it is necessary to find a method that can control both the vector insects and the pine wilt nematode, and solve the technical problems in the prevention and control of pine wilt disease. Summary of the Invention

[0007] The purpose of this invention is to provide an agent, method and application for controlling pine wood nematode. This agent and method can control both vector insects and pine wood nematode, and also overcome the problem of resin blockage.

[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0009] This invention provides an agent for controlling pine wilt disease, comprising the following components: bromocyanamide, chlorantraniliprole, abamectin, bark penetrant, and water.

[0010] Preferably, the bark penetrant is Licoline;

[0011] Preferably, the initial active ingredient content of the bromocyanamide is 10%;

[0012] Preferably, the initial active ingredient content of the chlorantraniliprole is 200 g / L; and the active ingredient content of the emamectin benzoate is 15–25 wt%.

[0013] Preferably, the volume ratio of bromocyanamide to water is 0.5–1.5:100; the volume ratio of chlorantraniliprole to water is 0.25–0.5:100; the mass-volume ratio of abamectin to water is 1–3 mg:100 ml; and the volume ratio of bark penetrant to water is 0.5–1.5:100.

[0014] The present invention also provides a method for controlling pine wilt disease, comprising the following steps: injecting an agent into a pine tree; wherein the agent is the agent for controlling pine wilt disease.

[0015] Preferably, the injection is a punch injection; the punch injection site is the bottom trunk or root of the pine tree; the bottom trunk of the pine tree is the trunk 10-30cm above the ground.

[0016] As a preferred method, when the diameter at breast height (DBH) of the pine tree is <15cm, the injection volume of the agent is 20-30ml / tree; when the DBH of the pine tree is 15-25cm, the injection volume of the agent is 30-50ml / tree; and when the DBH of the pine tree is >25cm, the injection volume of the agent is 60-80ml / tree.

[0017] Preferably, the injection time for the drug is from March to October.

[0018] This invention also provides the application of the aforementioned agent for controlling pine wilt disease in the preparation of products for controlling pine wilt disease and its vector insects.

[0019] The beneficial effects of this invention are:

[0020] This invention employs a dual control strategy against both vector insects and pine wilt nematodes. It utilizes a pesticide formulation that controls both insects and nematodes, along with a bark penetrant. The application method involves injecting the pesticide into holes drilled in the pine trunk and roots to kill both insects and nematodes, thus achieving the goal of controlling pine wilt nematodes. The bark penetrant helps to clear or reduce the blockage of the delivery channels by pine resin secreted by the pine tree after nematode infestation, allowing the pesticide to quickly reach all parts of the tree and exert its effect. This invention controls both vector insects and pine wilt nematodes, overcoming the problem of pine resin blockage in conventional injection methods. The pesticide works quickly and effectively; the operation is simple, saving labor costs, and the control cost is low, less than 5 yuan per tree per treatment. Detailed Implementation

[0021] This invention provides an agent for controlling pine wilt disease, comprising the following components: bromocyanamide, chlorantraniliprole, abamectin, bark penetrant, and water.

[0022] In this invention, the bark penetrant is Licolin; the product, developed using palm seed oil and its by-products as raw materials, has the characteristics of accelerating absorption, increasing efficacy, and resisting rain erosion;

[0023] In this invention, the broflanilide is a broflanilide suspension, and the initial active ingredient content of the broflanilide is 10%.

[0024] In this invention, the chlorantraniliprole is a chlorantraniliprole suspension, and the initial active ingredient content of the chlorantraniliprole is 200 g / L; the active ingredient content of the abamectin is 15-25 wt%, preferably 20 wt%.

[0025] In this invention, the volume ratio of bromocyanamide to water is 0.5–1.5:100, preferably 1:100; the volume ratio of chlorantraniliprole to water is 0.25–0.5:100, preferably 0.4:100; the mass-volume ratio of abamectin to water is 1–3 mg:100 ml, preferably 2 mg:100 ml; and the volume ratio of bark penetrant to water is 0.5–1.5:100, preferably 1:100.

[0026] The present invention also provides a method for controlling pine wilt disease, comprising the following steps: injecting an agent into a pine tree; wherein the agent is the agent for controlling pine wilt disease.

[0027] In this invention, the injection is a punch injection; the injection site is the bottom trunk or root of the pine tree; the bottom trunk of the pine tree is a trunk 10-30cm above the ground, preferably a trunk 20cm above the ground.

[0028] In this invention, when the diameter at breast height (DBH) of the pine tree is <15cm, the injection volume of the agent is 20-30ml / tree, preferably 25ml / tree; when the DBH of the pine tree is 15-25cm, the injection volume of the agent is 30-50ml / tree, preferably 40ml / tree; when the DBH of the pine tree is >25cm, the injection volume of the agent is 60-80ml / tree, preferably 70ml / tree.

[0029] In this invention, the injection time of the drug is from 3 months to 10 months.

[0030] This invention also provides the application of the aforementioned agent for controlling pine wilt disease in the preparation of products for controlling pine wilt disease and its vector insects.

[0031] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0032] The bark penetrant used in Examples 1-3 of this invention is Licorin, purchased from Guiyang Jinqian Agricultural Technology Co., Ltd., whose components are plant essential oil adjuvants, 100 ml / bottle; bromocyanamide was purchased from Guiyang Jinqian Agricultural Technology Co., Ltd., with an active ingredient content of 10%, 20 ml / packet or 1000 ml / bottle; chlorantraniliprole was purchased from Guiyang Jinqian Agricultural Technology Co., Ltd., with an active ingredient content of 200 g / L, 100 ml / bottle.

[0033] In 2023, severe pine wilt nematode damage was discovered in Mati Town, Bozhou District, Zunyi City, Guizhou Province (a provincial-level pine wilt nematode monitoring site), which was selected as an experimental site. The experimental site was divided into three experimental plots, with pine trees ranging from 15 cm to 45 cm in diameter at breast height. Pine wilt nematode control experiments were conducted in the experimental area from March to July 2023.

[0034] Example 1

[0035] In Hexin Group, Changyuan Village, Mati Town, Bozhou District, 150 trees were treated on March 28, 2023. The method used to control pine wilt nematode at the experimental site was as follows:

[0036] Preparation of pesticide for controlling pine wilt disease: 5000ml water, 100ml cyantraniliprole (initial active ingredient content 10%), 25ml chlorantraniliprole (active ingredient content 200g / l), 150mg abamectin (active substance content 20%), 100ml levofloxacin.

[0037] Application of the agent: Inject the agent into the base of the pine tree trunk and roots through holes. The injection amount per pine tree varies depending on the diameter at breast height (DBH). When the DBH of the pine tree is <15cm, the injection amount is 25ml / tree; when the DBH of the pine tree is 15-25cm, the injection amount is 40ml / tree; when the DBH of the pine tree is >25cm, the injection amount is 70ml / tree.

[0038] The average cost of pest control per tree is 4.50 yuan.

[0039] After 15 days of continuous application of the pesticide, the endangered trees resumed growth, and no live pine wilt nematodes were found in the samples.

[0040] Example 2

[0041] In Zhoujiawan, Yongyuan Group, Junhe Village, Mati Town, 245 plants were treated on April 25, 2023, using the same control method as in Example 1.

[0042] The average cost of pest control per tree is 4.50 yuan.

[0043] After 15 days of continuous application of the pesticide, the endangered trees resumed growth, and no live pine wilt nematodes were found in the samples.

[0044] Example 3

[0045] In Qinglong Temple, Qinglong Group, Yonghua Village, Mati Town, Bozhou District, 1200 plants were used in the control experiment on July 8, 2023.

[0046] The prevention and control methods are the same as in Example 1.

[0047] The average cost per tree is 4.50 yuan.

[0048] After 15 days of continuous application of the pesticide, the endangered trees resumed growth, and no live pine wilt nematodes were found in the samples.

[0049] As can be seen from the above embodiments, this invention provides an agent, method, and application for controlling pine wilt disease. It employs a dual control approach, targeting both vector insects and pine wilt disease. A compound pesticide formula is prepared to control both, while a bark penetrant is used. The pesticide is applied via a perforation injection method, injecting the pesticide into holes drilled in the pine trunk and roots to kill both vector insects and pine wilt disease, thereby achieving the goal of controlling pine wilt disease. This invention controls both vector insects and pine wilt disease, overcomes the problem of clogging by pine resin in ordinary injection methods, and allows the pesticide to work quickly and effectively. It is simple to operate, saves labor, and has low control costs, with each treatment costing less than 5 yuan.

[0050] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An agent for controlling pine wilt disease, characterized in that, The composition comprises bromocyclen, chlorantraniliprole, emamectin benzoate, bark penetrant and water. The bark penetrant is likoling. The volume ratio of bromocyclen to water is 0.5-1.5:100; the volume ratio of chlorantraniliprole to water is 0.25-0.5:100; the mass-volume ratio of emamectin benzoate to water is 1-3 mg:100 ml; the volume ratio of bark penetrant to water is 0.5-1.5:

100.

2. The agent for controlling the pine wood nematode according to claim 1, characterized by, The initial effective ingredient concentration of bromocyclen is 10%.

3. The agent for controlling the pine wood nematode according to claim 2, characterized by, The initial effective ingredient content of chlorantraniliprole is 200 g / l; the effective substance content of emamectin benzoate is 15-25 wt%.

4. A method of controlling the pine wood nematode, characterized by, The method comprises the step of injecting the medicine into the pine tree; the medicine is the pine wood nematode control medicine of claims 1-3.

5. The method for preventing and controlling pine wood nematode according to claim 4, wherein The injection is punching injection; the injection position is the bottom trunk or root of the pine tree; the bottom trunk of the pine tree is the trunk 10-30 cm from the ground.

6. The method for preventing and controlling pine wood nematode according to claim 5, wherein, When the diameter at breast height of the pine tree is <15 cm, the injection amount of the medicine is 20-30 ml per tree; when the diameter at breast height of the pine tree is 15-25 cm, the injection amount of the medicine is 30-50 ml per tree; when the diameter at breast height of the pine tree is >25 cm, the injection amount of the medicine is 60-80 ml per tree.

7. The method for controlling pine wilt disease according to claim 6, characterized in that, The injection time of the medicine is from March to October.

8. Use of the pine wood nematode control medicine of any one of claims 1-3 in the preparation of a product for controlling pine wood nematodes and / or vector insects.