Fresh wolfberry composition with function of improving vascular embolism and preparation method thereof

Abstract

The present invention relates to a fresh wolfberry composition having the function of improving vascular embolism and a preparation method thereof, and relates to the technical field of vascular embolism treatment. The fresh wolfberry composition comprises the following components, measured by weight: 600-800 parts of fresh wolfberry fruit, 100-200 parts of fresh roxburgh fruit, and 40-80 parts of northern lily of the valley. The present invention is the first to use fresh wolfberry fruit as the main raw material, combined with appropriate amounts of fresh roxburgh fruit and northern lily of the valley as raw materials. The three ingredients work synergistically to produce a fresh wolfberry composition having the function of improving vascular embolism, providing a new approach for preventing and treating vascular embolic diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of vascular embolism treatment, and in particular to a fresh wolfberry composition capable of improving vascular embolism and a preparation method thereof. Background Art

[0002] Vascular embolic disease primarily refers to blood coagulation within a vessel, which blocks the lumen and prevents blood flow. It can occur secondary to prolonged vascular spasm or due to hematologic factors, such as increased blood viscosity, platelet aggregation, and destruction, leading to vascular embolism. This can further lead to insufficient blood supply to the cardiovascular system, causing myocardial infarction, sudden ischemic death, stroke, coronary heart disease, and other conditions.

[0003] Fresh wolfberry is the fruit of the Solanaceae plant Lycium barbarum. It is a deciduous shrub belonging to the genus Lycium in the Solanaceae family. It is a medicinal and economic plant. Its mature and dried fruit is called wolfberry, which has the effects of nourishing yin and tonifying yang, nourishing the skin and beautifying, clearing the liver and improving eyesight. It can be used to improve symptoms such as deficiency of essence, waist and knee pain, etc.

[0004] The fresh fruit of sea buckthorn is the fruit of the sea buckthorn plant of the Rosaceae family. It has the effects of strengthening the stomach and helping digestion, and is mainly used to treat symptoms such as food accumulation and fullness.

[0005] Northern Liujinu is the dried whole herb of Yinxingcao, a plant of the Scrophulariaceae family. It has the effects of promoting blood circulation and removing blood stasis, dredging menstruation and relieving pain, cooling blood, stopping bleeding, clearing heat and removing dampness. It can be used to treat symptoms such as bruises, traumatic bleeding, blood stasis amenorrhea and irregular menstruation.

[0006] Currently, there is no report in the prior art on the combined use of fresh wolfberry fruit, fresh roxburghii fruit and Rhizoma Coptidis for treating vascular embolic diseases. Summary of the Invention

[0007] In order to solve the above problems, the present invention provides a fresh wolfberry composition having the function of improving vascular embolism and a preparation method thereof.

[0008] In a first aspect, the present invention provides a fresh wolfberry composition having the function of improving vascular embolism, wherein the fresh wolfberry composition comprises the following components in parts by weight:

[0009] 600-800 parts of fresh wolfberry fruit, 100-200 parts of fresh roxburghii fruit and 40-80 parts of northern liujiu fruit.

[0010] Furthermore, the fresh wolfberry composition comprises the following components in parts by weight:

[0011] 650-750 parts of fresh wolfberry fruit, 140-180 parts of fresh roxburghii fruit and 55-70 parts of northern liujiu fruit.

[0012] Furthermore, the fresh wolfberry composition comprises the following components in parts by weight:

[0013] 720 samples of fresh wolfberry fruit, 165 samples of fresh roxburghii fruit and 65 samples of northern liujiu fruit.

[0014] In a second aspect, the present invention provides a method for preparing the fresh wolfberry composition having the function of improving vascular embolism according to any one of the first aspects, the preparation method comprising the following steps:

[0015] The fresh wolfberry fruit and the fresh roxburgh pear fruit are subjected to microwave vacuum freeze drying to obtain dried wolfberry fruit and dried roxburgh pear;

[0016] The dried wolfberry, the dried roxburghii, the dried syzygium wilfordii and solid dry ice are mixed and crushed to obtain a mixture;

[0017] The mixed material is added into water and subjected to reduced pressure distillation extraction, and the distillate is collected and concentrated and dried to obtain the fresh wolfberry composition.

[0018] Furthermore, the steps of subjecting the fresh wolfberry fruit and the fresh roxburgh roxburgh fruit to microwave vacuum freeze drying to obtain dried wolfberry fruit and dried roxburgh roxburgh roxburgh include the following processes:

[0019] Fresh wolfberry and fresh sea buckthorn fruit are pre-frozen at a temperature of -10 to -15°C for 8 to 12 hours, and then placed in an environment of 35 to 45 Pa pressure, 200 to 300 W microwave power and -45 to -35°C for microwave vacuum freeze drying for 6.5 to 9 hours to obtain the dried wolfberry and the dried sea buckthorn fruit.

[0020] Furthermore, the operating parameters of the microwave vacuum freeze-drying include: pressure of 40 Pa, microwave power of 260 W, temperature of -38°C, and drying time of 7.5 hours.

[0021] Furthermore, the added weight of the solid dry ice is 5 to 10 times the total weight of the dried wolfberry, the dried roxburghii and the Herba Lysimachiae.

[0022] Furthermore, the mixed material is added to water for extraction by vacuum distillation, and the distillate is collected and concentrated and dried to obtain the fresh wolfberry composition, which comprises the following steps:

[0023] The mixed material is added to water (5 to 10) times the weight of the mixed material, and then a first reduced pressure distillation extraction is performed at a pressure of 800 to 900 Pa and a temperature of 24 to 28° C. When the volume of the distillate is half the volume of the initial amount of water added, the reduced pressure distillation is stopped to obtain a first distillate and a remaining liquid containing medicinal residues;

[0024] Adding water in an amount equal to the volume of the first distillate to the remaining liquid containing the medicinal residue, and then performing a second vacuum distillation extraction at a pressure of 3 to 6 kPa and a temperature of 40 to 45° C. When the volume of the distillate is half the volume of the initially added water, stopping the vacuum distillation to obtain a second distillate;

[0025] The first distillate and the second distillate are combined, concentrated, and dried to obtain the fresh wolfberry composition.

[0026] Furthermore, the working parameters of the first vacuum distillation extraction include: pressure of 850 Pa and temperature of 25°C.

[0027] Furthermore, the working parameters of the second reduced pressure distillation extraction include: pressure of 5 KPa and temperature of 42°C.

[0028] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0029] The embodiments of the present invention provide a fresh wolfberry composition having the function of improving vascular embolism and a preparation method thereof. The present invention is the first to use fresh wolfberry fruit as the main raw material and an appropriate amount of fresh roxburghii fruit and northern liuchi nux vomica as raw materials. The three ingredients work synergistically to prepare the fresh wolfberry composition having the function of improving vascular embolism, providing a new path for preventing and treating vascular embolic diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0032] Figure 1 The present invention provides a flow chart of a method for preparing a fresh wolfberry composition having the function of improving vascular embolism. DETAILED DESCRIPTION

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0034] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0035] In a first aspect, the present invention provides a fresh wolfberry composition having the function of improving vascular embolism, wherein the fresh wolfberry composition comprises the following components in parts by weight:

[0036] 600-800 parts of fresh wolfberry fruit, 100-200 parts of fresh roxburghii fruit and 40-80 parts of northern liujiu fruit.

[0037] The present invention is the first to use fresh wolfberry fruit as the main raw material and add an appropriate amount of fresh roxburghii fruit and northern liujiu as raw materials. The three ingredients work together to prepare a fresh wolfberry composition with the function of improving vascular embolism, providing a new path for preventing and treating vascular embolic diseases.

[0038] In some specific embodiments, the fresh wolfberry composition may consist only of the following raw materials, calculated by weight: 600-800 parts of fresh wolfberry fruits, 100-200 parts of fresh roxburghii fruits, and 40-80 parts of northern liuchi.

[0039] In the present invention, the fresh wolfberry fruit is the fruit of the Solanaceae plant wolfberry, which belongs to a deciduous shrub of the genus Lycium in the Solanaceae family. It is a medicinal and economic plant. Its mature and dry fruit is called wolfberry fruit, which has the effects of nourishing yin and tonifying yang, nourishing the skin and beautifying, clearing the liver and improving eyesight, and can be used to improve symptoms such as consumptive deficiency, soreness of the waist and knees. In some specific embodiments, the weight parts of the fresh wolfberry fruit can be 600 parts, 610 parts, 620 parts, 630 parts, 640 parts, 650 parts, 660 parts, 670 parts, 680 parts, 690 parts, 700 parts, 710 parts, 720 parts, 730 parts, 740 parts, 750 parts, 760 parts, 770 parts, 780 parts, 790 parts, 800 parts, etc.

[0040] In the present invention, the fresh fruit of Rosa roxburghii is the fruit of the Rosa roxburghii plant of the Rosaceae family, has the effects of strengthening the stomach and promoting digestion, and is mainly used to treat symptoms such as food stagnation and fullness. In some specific embodiments, the weight ratio of the fresh fruit of Rosa roxburghii can be 100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts, 170 parts, 180 parts, 190 parts, 200 parts, etc.

[0041] In the present invention, the northern Liujinu is the dried whole herb of Yinxingcao of the Scrophulariaceae family, which has the effects of promoting blood circulation and removing blood stasis, dredging menstruation and relieving pain, cooling blood, stopping bleeding, and clearing away heat and dampness. It can be used to treat symptoms such as traumatic injuries, traumatic bleeding, blood stasis amenorrhea and irregular menstruation. In some specific embodiments, the weight parts of the Northern Liujinu can be 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, 51 parts, 52 parts, 53 parts, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, 61 parts, 62 parts, 63 parts, 64 parts, 65 parts, 66 parts, 67 parts, 68 parts, 69 parts, 70 parts, 71 parts, 72 parts, 73 parts, 74 parts, 75 parts, 76 parts, 77 parts, 78 parts, 79 parts, 80 parts, etc.

[0042] Preferably, the fresh wolfberry composition comprises the following components in parts by weight:

[0043] 650-750 parts of fresh wolfberry fruit, 140-180 parts of fresh roxburghii fruit and 55-70 parts of northern liujiu fruit.

[0044] More preferably, the fresh wolfberry composition comprises the following components in parts by weight:

[0045] 720 samples of fresh wolfberry fruit, 165 samples of fresh roxburghii fruit and 65 samples of northern liujiu fruit.

[0046] It should be noted that, unless otherwise specified or specified, the components and raw materials involved in the fresh wolfberry composition with the function of improving vascular embolism provided by the embodiments of the present invention can be directly commercially available products.

[0047] In a second aspect, based on a general inventive concept, the present invention provides a method for preparing the fresh wolfberry composition having the function of improving vascular embolism as described in any one of the first aspects, such as Figure 1 As shown, the preparation method comprises the following steps:

[0048] The fresh wolfberry fruit and the fresh roxburgh pear fruit are subjected to microwave vacuum freeze drying to obtain dried wolfberry fruit and dried roxburgh pear;

[0049] The dried wolfberry, the dried roxburghii, the dried syzygium wilfordii and solid dry ice are mixed and crushed to obtain a mixture;

[0050] The mixed material is added into water and subjected to reduced pressure distillation extraction, and the distillate is collected and concentrated and dried to obtain the fresh wolfberry composition.

[0051] The preparation method of the fresh wolfberry composition with the function of improving vascular embolism provided by the present invention is achieved by combining "microwave vacuum freeze drying", "adding solid dry ice for mixing and crushing", and "low-temperature reduced pressure distillation extraction", which fully retains the active components of each raw material component, making the fresh wolfberry composition more effective in improving vascular embolism. At the same time, the preparation method is simple to operate, does not require additional specific equipment, and is suitable for industrial production. At the same time, the preparation method of the fresh wolfberry composition with the function of improving vascular embolism is based on the fresh wolfberry composition with the function of improving vascular embolism described in any one of the first aspects above, and therefore has at least all the beneficial effects brought about by the technical solutions of the above embodiments, which will not be repeated here.

[0052] In some specific embodiments, the step of subjecting fresh wolfberry fruit and fresh roxburgh roxburgh fruit to microwave vacuum freeze-drying to obtain dried wolfberry fruit and dried roxburgh roxburgh roxburgh comprises the following process:

[0053] Fresh wolfberry and fresh sea buckthorn fruit are pre-frozen at a temperature of -10 to -15°C for 8 to 12 hours, and then placed in an environment of 35 to 45 Pa pressure, 200 to 300 W microwave power and -45 to -35°C for microwave vacuum freeze drying for 6.5 to 9 hours to obtain the dried wolfberry and the dried sea buckthorn fruit.

[0054] Preferably, the operating parameters of the microwave vacuum freeze-drying include: pressure of 40 Pa, microwave power of 260 W, temperature of -38°C, and drying time of 7.5 hours.

[0055] In some specific embodiments, the added weight of the solid dry ice is 5 to 10 times the total weight of the dried wolfberry, the dried roxburghii and the Herba Lysimachiae.

[0056] Preferably, the added weight of the solid dry ice is 8 times the total weight of the dried wolfberry, the dried roxburghii and the Herba Lysimachiae.

[0057] In some specific embodiments, the step of adding the mixture to water and performing vacuum distillation extraction, collecting the distillate and concentrating and drying it to obtain the fresh wolfberry composition comprises the following process:

[0058] The mixed material is added to water (5 to 10) times the weight of the mixed material, and then a first reduced pressure distillation extraction is performed at a pressure of 800 to 900 Pa and a temperature of 24 to 28° C. When the volume of the distillate is half the volume of the initial amount of water added, the reduced pressure distillation is stopped to obtain a first distillate and a remaining liquid containing medicinal residues;

[0059] Adding water in an amount equal to the volume of the first distillate to the remaining liquid containing the medicinal residue, and then performing a second vacuum distillation extraction at a pressure of 3 to 6 kPa and a temperature of 40 to 45° C. When the volume of the distillate is half the volume of the initially added water, stopping the vacuum distillation to obtain a second distillate;

[0060] The first distillate and the second distillate are combined, concentrated, and dried to obtain the fresh wolfberry composition.

[0061] Preferably, the working parameters of the first vacuum distillation extraction include: pressure of 850 Pa and temperature of 25°C.

[0062] Preferably, the working parameters of the second reduced pressure distillation extraction include: pressure of 5 KPa and temperature of 42°C.

[0063] It should be noted that the operating steps involved in the preparation method of the fresh wolfberry composition with the function of improving vascular embolism provided by the embodiment of the present invention, unless otherwise specified or explained, can be carried out in accordance with conventional methods in the art or using existing equipment, and will not be described in detail here.

[0064] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended to illustrate the present invention only and are not intended to limit the scope of the invention. The experimental methods in the following examples where specific conditions are not specified are generally measured in accordance with national standards. If there are no corresponding national standards, then the methods are carried out in accordance with general international standards, conventional conditions, or the conditions recommended by the manufacturer.

[0065] Example 1

[0066] This example provides a fresh wolfberry composition with the function of improving vascular embolism. Calculated by weight (each weight part is 10g), the fresh wolfberry composition includes the following components: 600 parts of fresh wolfberry fruit, 100 parts of fresh sea buckthorn fruit and 40 parts of northern Liujinu.

[0067] The preparation method of the fresh wolfberry composition comprises the following steps:

[0068] Step (1), microwave vacuum freeze drying: pre-freeze the fresh wolfberry fruit and the fresh roxburghii fruit at a temperature of -10°C for 12 hours, and then place them in a -35°C environment with a pressure of 35 Pa, a microwave power of 300 W, and microwave vacuum freeze drying for 9 hours to obtain the dried wolfberry and the dried roxburghii fruit;

[0069] Step (2), crushing and mixing: mixing and crushing the dried wolfberry obtained in step (1), the dried roxburghii roxburghii obtained in step (1), and Herba Lycopodii flavescentis and solid dry ice (the weight of the solid dry ice added is 8 times the total weight of the dried wolfberry, the dried roxburghii roxburghii and the Herba Lycopodii flavescentis) to obtain a mixture;

[0070] Step (3), extraction by reduced pressure distillation: adding the mixture obtained in step (2) to water accounting for 8 times the weight of the mixture, then performing a first reduced pressure distillation extraction at a pressure of 800 Pa and a temperature of 28° C., stopping the reduced pressure distillation when the volume of the distillate is half the volume of the initial amount of water added, and obtaining a first distillate and a residual liquid containing the medicinal residue; adding water in an amount equal to the volume of the first distillate to the residual liquid containing the medicinal residue, and then performing a second reduced pressure distillation extraction at a pressure of 3 KPa and a temperature of 45° C., stopping the reduced pressure distillation when the volume of the distillate is half the volume of the initial amount of water added, and obtaining a second distillate; combining the first distillate and the second distillate, performing reduced pressure concentration, and vacuum drying to a constant weight, to obtain the fresh wolfberry composition.

[0071] Example 2

[0072] This example provides a fresh wolfberry composition with the function of improving vascular embolism. Calculated by weight (each weight portion is 10g), the fresh wolfberry composition includes the following components: 800 parts of fresh wolfberry fruit, 200 parts of fresh sea buckthorn fruit and 80 parts of northern Liujinu.

[0073] The preparation method of the fresh wolfberry composition comprises the following steps:

[0074] Step (1), microwave vacuum freeze drying: pre-freeze the fresh wolfberry fruit and the fresh roxburghii fruit at a temperature of -15°C for 8 hours, and then place them in a -45°C environment at a pressure of 45 Pa, a microwave power of 200 W, and perform microwave vacuum freeze drying for 6.5 hours to obtain the dried wolfberry and the dried roxburghii fruit;

[0075] Step (2), crushing and mixing: mixing and crushing the dried wolfberry obtained in step (1), the dried roxburghii roxburghii obtained in step (1), and Herba Lycopodii flavescentis and solid dry ice (the weight of the solid dry ice added is 8 times the total weight of the dried wolfberry, the dried roxburghii roxburghii and the Herba Lycopodii flavescentis) to obtain a mixture;

[0076] Step (3), extraction by reduced pressure distillation: adding the mixture obtained in step (2) to water accounting for 8 times the weight of the mixture, then performing a first reduced pressure distillation extraction at a pressure of 900 Pa and 24° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a first distillate and a residual liquid containing the medicinal residue; adding water in an amount equal to the volume of the first distillate to the residual liquid containing the medicinal residue, then performing a second reduced pressure distillation extraction at a pressure of 6 KPa and 40° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a second distillate; combining the first distillate and the second distillate, performing reduced pressure concentration, and vacuum drying to constant weight to obtain the fresh wolfberry composition.

[0077] Example 3

[0078] This example provides a fresh wolfberry composition with the function of improving vascular embolism and a preparation method thereof. The only difference from Example 1 is that: in the preparation method of the fresh wolfberry composition, the operating parameters of the microwave vacuum freeze-drying in step (1) are adjusted to: pressure of 40 Pa, microwave power of 260 W, temperature of -38°C, and drying time of 7.5 hours; in the preparation method of the fresh wolfberry composition, the operating parameters of the first reduced pressure distillation extraction in step (3) are adjusted to: pressure of 850 Pa, temperature of 25°C, and the operating parameters of the second reduced pressure distillation extraction are adjusted to: pressure of 5 KPa, temperature of 42°C; the remaining steps and parameters are the same.

[0079] Example 4

[0080] This example provides a fresh wolfberry composition with the function of improving vascular embolism and a preparation method thereof. The only difference from Example 1 is that: in parts by weight (each part by weight is 10 g), the fresh wolfberry composition includes the following components: 650 parts of fresh wolfberry fruit, 140 parts of fresh roxburghii fruit and 55 parts of northern liujiu; the remaining steps and parameters are the same.

[0081] Example 5

[0082] This example provides a fresh wolfberry composition with the function of improving vascular embolism. Calculated by weight (each weight part is 10g), the fresh wolfberry composition includes the following components: 720 parts of fresh wolfberry fruit, 165 parts of fresh sea buckthorn fruit and 65 parts of northern Liujinu.

[0083] The preparation method of the fresh wolfberry composition comprises the following steps:

[0084] Step (1), microwave vacuum freeze drying: pre-freeze the fresh wolfberry fruit and the fresh roxburghii fruit at a temperature of -12°C for 9 hours, and then place them in an environment of 40Pa pressure, a microwave power of 260W and -38°C for microwave vacuum freeze drying for 7.5 hours to obtain the dried wolfberry and the dried roxburghii fruit;

[0085] Step (2), crushing and mixing: mixing and crushing the dried wolfberry obtained in step (1), the dried roxburghii roxburghii obtained in step (1), and Herba Lycopodii flavescentis and solid dry ice (the weight of the solid dry ice added is 8 times the total weight of the dried wolfberry, the dried roxburghii roxburghii and the Herba Lycopodii flavescentis) to obtain a mixture;

[0086] Step (3), extraction by reduced pressure distillation: adding the mixture obtained in step (2) to water accounting for 8 times the weight of the mixture, then performing a first reduced pressure distillation extraction at a pressure of 850 Pa and 25° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a first distillate and a remaining liquid containing the medicinal residue; adding water in an amount equal to the volume of the first distillate to the remaining liquid containing the medicinal residue, then performing a second reduced pressure distillation extraction at a pressure of 5 KPa and 42° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a second distillate; combining the first distillate and the second distillate, performing reduced pressure concentration, and vacuum drying to constant weight to obtain the fresh wolfberry composition.

[0087] Comparative Example 1

[0088] This example provides a fresh wolfberry composition and a preparation method thereof, which differs from Example 1 only in that the amount of fresh wolfberry fruit is adjusted to 700 parts, and the amount of fresh roxburghii fruit is adjusted to 0 parts; the remaining steps and parameters are the same.

[0089] Comparative Example 2

[0090] This example provides a fresh wolfberry composition and a preparation method thereof, which differs from Example 1 only in that the amount of fresh wolfberry is adjusted to 640 parts, and the amount of Northern Liujinu is adjusted to 0 parts; the remaining steps and parameters are the same.

[0091] Comparative Example 3

[0092] This example provides a fresh wolfberry composition and a preparation method thereof, which differs from Example 1 only in that the working parameters of the first reduced pressure distillation extraction in step (3) of the preparation method of the fresh wolfberry composition are adjusted to: pressure of 15 kPa, temperature of 60°C, and the working parameters of the second reduced pressure distillation extraction are adjusted to: pressure of 15 kPa, temperature of 60°C; the remaining steps and parameters are the same.

[0093] Comparative Example 4

[0094] This example provides a fresh wolfberry composition, which includes the following components in parts by weight (each part by weight is 10g): 600 parts of fresh wolfberry fruits, 100 parts of fresh roxburghii fruits and 40 parts of northern liujiu.

[0095] The preparation method of the above-mentioned fresh wolfberry composition includes the following steps: mixing and crushing commercially available dried wolfberries, commercially available dried roxburghii fruits and northern liuchi nux vomica to obtain a mixture; then adding the mixture into water accounting for 8 times the weight of the mixture and performing reflux extraction for 3 hours, reflux extraction twice, filtering and combining the extracts, concentrating the extracts under reduced pressure, and vacuum drying to constant weight to obtain the fresh wolfberry composition.

[0096] Test Case

[0097] In this example, the in vitro thrombolytic effect test was performed on the fresh wolfberry compositions obtained in Examples 1 to 5 and Comparative Examples 1 to 4.

[0098] Sample solution preparation: The fresh wolfberry compositions obtained in Examples 1 to 5 and Comparative Examples 1 to 4 were respectively dissolved in methanol to obtain 0.2 mg / ml methanol solutions of the fresh wolfberry compositions as sample test solutions; in addition, 0.1 mg / ml methanol solution of aspirin was used as a positive control sample solution, and physiological saline was used as a blank control sample solution.

[0099] Test method:

[0100] 1) Blood Processing: Weigh a labeled microcentrifuge tube and record its weight as M1. Add 500 μL of freshly collected whole blood from SPF male Sprague-Dawley rats (220-240 g) to each microcentrifuge tube and incubate at 37°C for 90 min to allow the blood to fully clot. After the blood has coagulated, carefully separate the supernatant serum without disrupting the clot. Weigh the tube and record its weight as M2. Calculate the mass of the clot according to Formula 1 and record it as M3.

[0101] 2) In vitro thrombolysis assay: The experiment was divided into a blank control group (1 group, 5 centrifuge tubes in each group were given 500ul of blank control sample solution), a positive control group (1 group, 5 centrifuge tubes in each group were given 500ul of positive control sample solution), and a drug-treated group (9 groups, 5 centrifuge tubes in each group were given 500ul of sample test solution). All centrifuge tubes were incubated at 37°C for 150 min, and the clot lysis was observed. After the incubation is completed, the centrifuge tube is taken out, and the upper liquid is carefully precipitated without destroying the blood clot. After completion, the centrifuge tube is weighed and recorded as M4, and the weight of the blood clot after lysis is calculated according to Formula 2 and recorded as M5; m5 = m4 - m1); finally, the thrombolysis rate (%) of the test sample in each centrifuge tube is calculated according to Formula 3 and the arithmetic mean of the blood clot lysis rate (%) is calculated to obtain the average thrombolysis rate (%). The test results are shown in Table 1; wherein, Formula 1: M3 = M2 - M1; Formula 2: M5 = M4 - M1; Formula 3: Blood clot lysis rate (%) = ((M3 - M5) / M3) × 100%.

[0102] Table 1

[0103] Test samples Average thrombolysis rate (%) Blank control group 17.6％ Positive control group 48.9％ Example 1 36.5％ Example 2 38.1％ Example 3 42.7％ Example 4 43.9％ Example 5 54.2％ Comparative Example 1 19.3％ Comparative Example 2 21.8％ Comparative Example 3 27.0％ Comparative Example 4 22.4％

[0104] As can be seen from Table 1, compared with the comparative example, the fresh wolfberry composition provided in Examples 1 to 5 of the present application has a better in vitro thrombolytic effect; at the same time, the fresh wolfberry composition provided in Example 5 has the best in vitro thrombolytic effect, which is slightly better than the aspirin methanol solution as the positive control sample solution.

[0105] In summary, the present invention is the first to use fresh wolfberry fruit as the main raw material and an appropriate amount of fresh roxburghii fruit and northern liuchi nux vomica as raw materials. The three ingredients work together to produce a fresh wolfberry composition with the function of improving vascular embolism, providing a new path for preventing and treating vascular embolic diseases.

[0106] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in a range format is only for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention; therefore, the range description should be considered to have specifically disclosed all possible subranges and single numerical values within the range. For example, the description of a range from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. In addition, whenever a numerical range is indicated herein, it is intended to include any cited numeral (fractional or integer) within the indicated range.

[0107] In the present invention, unless otherwise specified, the directional words used, such as "upper" and "lower", specifically refer to the directions of the drawings in the accompanying drawings. In addition, in the description of the present specification, the terms "including", "comprising", etc. mean "including but not limited to". In this article, relational terms such as "first" and "second" are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. In this article, "and / or" describes the association relationship of associated objects, indicating that three relationships may exist. For example, A and / or B can mean: A exists alone, A and B exist at the same time, and B exists alone. Wherein A and B can be singular or plural. In this article, "at least one" means one or more, and "plurality" means two or more. "At least one", "at least one of the following" or similar expressions refer to any combination of these items, including any combination of single or plural items. For example, "at least one of a, b, or c", or "at least one of a, b and c", can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, c can be single or multiple.

[0108] The foregoing description is intended only to provide specific embodiments of the present invention, which will enable those skilled in the art to understand and implement the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not intended to be limited to the embodiments shown herein, but is intended to be accorded the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A fresh wolfberry composition for improving vascular embolism, characterized in that: The fresh wolfberry composition is prepared from the following raw materials in parts by weight: 720 parts of fresh wolfberry fruit, 165 parts of fresh roxburghii fruit and 65 parts of northern liujiu; The preparation method of the fresh wolfberry composition comprises the following steps: Step (1): pre-freeze the fresh wolfberry fruit and the fresh sea buckthorn fruit at -12°C for 9 hours, and then place them in a -38°C environment at a pressure of 40 Pa, a microwave power of 260 W, and perform microwave vacuum freeze drying for 7.5 hours to obtain the dried wolfberry fruit and the dried sea buckthorn fruit; Step (2): mixing and grinding the dried wolfberry obtained in step (1), the dried roxburghii roxburghii obtained in step (1), and Herba Lycoris radiatae and solid dry ice, wherein the weight of the solid dry ice added is 8 times the total weight of the dried wolfberry, the dried roxburghii roxburghii and Herba Lycoris radiatae, to obtain a mixture; Step (3): adding the mixture obtained in step (2) to water accounting for 8 times the weight of the mixture, and then performing a first reduced pressure distillation extraction at a pressure of 850 Pa and 25° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a first distillate and a residual liquid containing the medicinal residue; adding water of the same volume as the first distillate to the residual liquid containing the medicinal residue, and then performing a second reduced pressure distillation extraction at a pressure of 5 KPa and 42° C., when the volume of the distillate is half the volume of the initial added amount of the water, stopping the reduced pressure distillation to obtain a second distillate; combining the first distillate and the second distillate, performing reduced pressure concentration, and vacuum drying to constant weight to obtain the fresh wolfberry composition.

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