An enhanced fluorescent probe that specifically recognizes mercury ions, its synthesis method, and its applications.

By synthesizing an enhanced fluorescent probe FS-Hg that specifically recognizes mercury ions, the problems of insufficient specificity and response time of existing fluorescent probes in recognizing mercury ions are solved, realizing high-sensitivity, low-cost, real-time monitoring of mercury ions in food, which is suitable for food safety testing.

CN117720549BActive Publication Date: 2026-03-06FUJIAN NORMAL UNIV
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Patent Information

Application Number
CN202311696168.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-12-11
Publication Date
2026-03-06
Estimated Expiration
2043-12-11

AI Technical Summary

Technical Problem

Existing fluorescent probes have drawbacks such as weak specificity, long response time, and insufficient anti-interference ability when identifying mercury ions, which limit their application in food safety testing.

Method used

An enhanced fluorescent probe FS-Hg that specifically recognizes mercury ions was designed and synthesized. The probe was formed by reacting Rhodamine B with hydrated hydrazine and 3-pyridyl isothiocyanate to form a fluorescent probe with the molecular formula C34H35N6O2S. The probe only causes a visible color change and strong fluorescence response after recognizing mercury ions.

Benefits of technology

It enables real-time monitoring of mercury ions with visualization, low detection limit, and high sensitivity. It has extremely high sensitivity, good selectivity, and stable light energy characteristics. The synthesis method is simple and low-cost, and it is suitable for safety assessment of mercury contamination in food.

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Abstract

This invention discloses a method for synthesizing and applying an enhanced fluorescent probe that specifically recognizes mercury ions. The probe is based on the rhodamine structure and modified with a pyridyl isothiocyanate structure, with the molecular formula C0.05. 34 H 35 N6O2S, labeled FS-Hg, is a fluorescent probe that can monitor mercury ions in real time in N,N-dimethylformamide solution. It only causes a visible color change (from colorless to bright pink) and new spectral bands upon recognition of mercury ions. The probe also causes a sharp increase in absorbance at 568 nm and a sharp increase in fluorescence intensity at 594 nm upon mercury ion recognition. The mercury ion recognition process exhibits concentration dependence, extremely high sensitivity, good selectivity, and stable sensing characteristics. This probe can efficiently detect mercury ion concentration in aquatic products, which is of great significance for on-site detection and large-scale screening of heavy metals in food safety.
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