A fluorescent probe for detecting cysteine ​​and its preparation method and use method

Through the ratio-type fluorescence probe BDP-4SPy, a benzoBODIPY fluorophore reacts with cysteine ​​to generate a specific fluorescence signal, solving the problem that existing fluorescence probes are susceptible to environmental influences, and achieving high sensitivity and high accuracy detection of cysteine.

CN118702714BActive Publication Date: 2025-08-29ZHEJIANG SCI-TECH UNIV
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Patent Information

Application Number
CN202411009255.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-07-26
Publication Date
2025-08-29
Estimated Expiration
2044-07-26

AI Technical Summary

Technical Problem

When detecting cysteine, existing fluorescent probes are susceptible to factors such as probe concentration, light source intensity, detection temperature and instrument sensitivity, resulting in inaccurate detection results.

Method used

The ratio-type fluorescent probe BDP-4SPy is used to use benzoBODIPY as a fluorophore, and the sulfhydryl nucleophilic substitution reaction of the sulfhydryl pyridine moiety and the cysteine ​​are carried out, and a specific fluorescence signal is generated. The fluorescence intensity ratios at 550 nm and 590 nm are used to quantitatively detect cysteine.

Benefits of technology

The specific detection of cysteine ​​is achieved, which can overcome the influence of environmental and instrumental factors, improve the sensitivity and accuracy of the detection, and is easy to operate and low cost.

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Abstract

The present invention discloses a fluorescent probe for detecting cysteine, and its preparation and use methods. The present invention designs a ratiometric fluorescent probe BDP-4SPy based on benzo-BodiPy and 4-mercaptopyridine. The thiopyridine portion of the probe is a good leaving group. It undergoes nucleophilic substitution with the thiol group of Cys to generate BDP-S-Cys, which is then subjected to intramolecular rearrangement to generate BDP-N-Cys, thereby causing a change in the probe fluorescence. The ratiometric fluorescent probe of the benzo-BodiPy dye provided by the present invention has good response performance to cysteine, can achieve sensitive, rapid, and quantitative detection of trace cysteine ​​in a sample, and has the advantages of simple operation, low cost, sensitive response, and easy promotion and application.
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