Additive for preventing and treating pig foot and leg diseases, pig feed, preparation method and application

Abstract

The invention discloses an additive for preventing and treating pig foot and leg diseases. The additive is prepared from at least composite strain fermentation liquor and a traditional Chinese medicine composition, the composite strain fermentation liquor comprises bacillus subtilis fermentation liquor, lactobacillus lactis fermentation liquor and saccharomyces cerevisiae fermentation liquor; the traditional Chinese medicine composition at least comprises fructus psoraleae, rhizoma atractylodis macrocephalae, poria cocos, frankincense, radix curcumae and radix rehmanniae recen, the bitter taste of the medicinal materials can be removed or weakened through microbial fermentation of the traditional Chinese medicines, so that the taste of the traditional Chinese medicines added into the pig feed is improved, and adverse effects on the feed intake of pigs are avoided; more importantly, the probiotics and the traditional Chinese medicine composition are synergistically compounded, so that the traditional Chinese medicine composition can be used for preventing nutritional limb and foot diseases and treating mild symptoms of the nutritional limb and foot diseases and the bacterial limb and foot diseases. The invention further discloses a preparation method of the additive, a pig feed containing the additive and application of the pig feed.

Description

Technical Field

[0001] The invention belongs to the technical field of pig breeding, and in particular relates to an additive for preventing and treating pig limb and hoof diseases. The invention also relates to pig feed containing the additive, and a preparation method and application of the additive. Background Art

[0002] Porcine limb and hoof disease is a general term for a class of diseases, which generally refers to movement disorders in the pig's hooves and limbs or ulcers, cracks, erosions and other symptoms in the hooves and limbs. It mainly includes nutritional limb and hoof disease, bacterial limb and hoof disease, viral limb and hoof disease, hereditary limb and hoof disease, etc.

[0003] It is difficult for sick pigs to carry out normal daily activities (such as eating, walking, etc.). When the symptoms are more serious, the treatment effect is not good. If it is a contagious bacterial limb and hoof disease, it may also infect other healthy pigs, which has an adverse effect on the production efficiency of the pig herd. Pig limb and hoof disease has become one of the important reasons for the elimination of breeding pigs in large-scale pig farms, seriously damaging the economic benefits of the pig farming industry. Viral limb and hoof disease progresses quickly, and hereditary limb and hoof disease is a genetic problem. It is difficult to prevent it in daily feeding or treat it in time when the symptoms are mild. However, for nutritional limb and hoof disease and bacterial limb and hoof disease, prevention is more important than treatment and has a higher cost-effectiveness.

[0004] In order to effectively prevent pig limb and hoof diseases, the patent document with announcement number CN113040283B discloses a premix feed and its application for protecting the health of sow limbs and hooves. The main ingredients of the premix feed are manganese gluconate, zinc lactate, copper citrate, phytase and rice husk powder. The premix feed can significantly reduce the incidence of sow heel disease and the incidence of the heel-sole junction.

[0005] The patent document with announcement number CN109924342B discloses a compound feed, a compound feed additive and a method for preparing a compound feed additive for reducing limb and hoof diseases in sows, comprising: sintering dried rice husks in an anaerobic environment to form carbonized rice husks; then adding the carbonized rice husks to a compound enzyme preparation for enzymolysis, taking them out and draining them, then pouring them into a composite trace element aqueous solution for soaking, then taking them out, freeze-drying and crushing them, and after the crushing is completed, adding calcium dihydrogen phosphate and calcium lactate and mixing them evenly to obtain a first material; crushing green whole-plant corn and then mixing them evenly with a fermentation agent and then sealing and fermenting them, after the fermentation is completed, adding the first material, alfalfa powder and compound vitamins and mixing them evenly, then freeze-drying and crushing them, and then adding carboxymethyl dextran and sodium tripolyphosphate and mixing them evenly to obtain a compound feed additive, which can effectively reduce the occurrence of limb and hoof diseases in sows.

[0006] However, the above-mentioned prior art mainly designs and proportions nutritional elements such as vitamins and minerals, which have a good preventive and therapeutic effect on farmed pigs suffering from nutritional limb and hoof diseases, but the preventive and therapeutic effect on bacterial limb and hoof diseases is relatively limited. On the other hand, the phenomenon of antibiotic abuse in pig farms is relatively serious, so it is not appropriate to use antibiotics for prevention or treatment at the stage of prevention or when the symptoms are relatively mild.

[0007] The applicant has deep roots in the field of animal husbandry technology and has an in-depth understanding of downstream farms. He believes that it is necessary to develop an antibiotic-free additive that can simultaneously prevent and treat nutritional and bacterial limb and hoof diseases, and apply it to daily feeding in farms. Summary of the invention

[0008] In view of the problems in the related technology, the present invention proposes an additive for preventing and treating pig limb and hoof diseases to overcome the above-mentioned technical problems existing in the existing related technology; the present invention also discloses a preparation method of the additive, pig feed containing the additive and application.

[0009] The technical solution of the present invention is achieved in this way:

[0010] An additive for preventing and treating pig limb and hoof diseases, wherein the raw materials for preparation at least include a composite bacterial fermentation liquid and a traditional Chinese medicine composition;

[0011] The composite bacterial fermentation liquid comprises Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid;

[0012] The traditional Chinese medicine composition at least comprises psoralea corylifolia, atractylodes macrocephala, tuckahoe, frankincense, curcuma and raw rehmannia root.

[0013] Compared with the prior art, the present invention can remove or weaken the bitterness of Chinese medicine by fermenting Chinese medicine with microorganisms, thereby improving the taste of Chinese medicine after being added to pig feed and avoiding adverse effects on the pig's feed intake; more importantly, the present invention compounds probiotics and Chinese medicine compositions, so that the present invention can prevent and treat mild cases of nutritional limb and hoof diseases and bacterial limb and hoof diseases.

[0014] Preferably, the weight ratio of the Bacillus subtilis fermentation broth, the Lactobacillus lactis fermentation broth and the Saccharomyces cerevisiae fermentation broth is 0.8-1.0:1.0-1.2:0.8-1.0.

[0015] Preferably, the Chinese medicine composition comprises at least the following raw materials in parts by weight:

[0016] 40-70 parts of Psoralea corylifolia, 40-60 parts of Atractylodes macrocephala, 40-60 parts of Poria cocos, 30-50 parts of frankincense, 30-50 parts of Curcuma aromatica and 30-50 parts of Rehmannia root.

[0017] More preferably, the Chinese medicine composition comprises the following raw materials in parts by weight:

[0018] 55-70 parts of Psoralea corylifolia, 45-53 parts of Atractylodes macrocephala, 45-53 parts of Poria cocos, 35-43 parts of frankincense, 35-43 parts of Curcuma aromatica, 35-43 parts of Rehmannia root, 20-33 parts of Dipsacus asper, 20-33 parts of Alisma orientalis, 20-33 parts of White Peony Root, 20-33 parts of Myrrha and 20-33 parts of Licorice.

[0019] From the perspective of traditional Chinese medicine, the spleen governs the limbs and muscles, so spleen damage can cause swelling or damage to the joints and muscles of the pig's limbs. The spleen is the foundation of acquired constitution. If the spleen is not functioning well, the body's energy supply will be insufficient. The kidney governs the bones and reproductive system, which is the foundation of congenital constitution. During pregnancy and childbirth, sows will continuously consume their essence and kidney energy, which will have adverse effects on bones and hoofs. The liver governs the tendons, and the kidney is water, the liver is wood, and water produces wood. When the kidney water is insufficient, the liver wood is affected, and then the tendons of the limbs will have adverse symptoms. Therefore, sows will have difficulty standing and have diarrhea. Therefore, the present invention focuses on the design of Chinese medicine formula from the aspects of strengthening the spleen, consolidating the kidney, and soothing the liver, so as to achieve the effect of strengthening bones and preventing pig limb and hoof disease. Specifically, psoralea corylifolia, Dipsacus asper and Rehmannia glutinosa are mainly used to strengthen the kidney and strengthen the kidney, and then have a bone strengthening effect on sows. The main functions of Atractylodes macrocephala, Poria cocos, Alisma orientalis and Paeonia lactiflora are to strengthen the spleen and remove dampness, so that sows have better nutrient absorption efficiency; frankincense, Curcuma aromatica and Myrrh work together to soothe the liver and strengthen the tendons, while relieving pain and promoting muscle growth, thereby reducing the pain of pigs with limb and hoof disease; Licorice is mainly used to reconcile various medicines. Through the above Chinese medicine reconciliation, the overall nutrient absorption capacity and body immunity of the sow are enhanced, the tendons and bones are strengthened, and it is suitable for the prevention and mild treatment of nutritional limb and hoof disease and bacterial limb and hoof disease.

[0020] The preparation method of the additive for preventing and treating pig limb and hoof disease comprises the following steps:

[0021] 1) reviving and culturing Bacillus subtilis, Lactobacillus lactis and Saccharomyces cerevisiae to obtain Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid;

[0022] 2) mixing Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid according to a preset weight ratio to obtain a composite bacterial fermentation liquid;

[0023] 3) preparing the Chinese medicine composition according to the preset components and weight proportions, and crushing the mixture;

[0024] 4) preparing fermentation raw materials and performing fermentation, wherein the fermentation raw materials at least include the composite bacterial fermentation liquid and the traditional Chinese medicine composition;

[0025] 5) After the fermentation is completed, the product is bottled to obtain an additive for preventing and treating pig limb and hoof diseases.

[0026] Preferably, step 1) includes the following steps:

[0027] 1-1) Inoculate Bacillus subtilis, Lactobacillus lactis and Saccharomyces cerevisiae into suitable culture medium respectively, set the shaking table at 30-37°C and 120-180 rpm / min, and perform culture and resurrection until the number of viable bacteria is ≥0.8×10 8 CFU / ml, as the primary seed solution;

[0028] 1-2) Transfer each primary seed solution to a fermentation tank with an inoculation amount of 5-8% of the total mass of the fermentation product, a culture temperature of 25-30°C, a stirring speed of 200-250rpm, and fermentation until the bacterial content is ≥0.8×10 8 CFU / ml, as secondary seed solution;

[0029] 1-3) Transfer each secondary seed solution to the production tank, inoculate it at 10-15% of the total mass of the fermentation product, culture at 25-30°C, stir at 120-180 rpm, and ferment until the bacterial content is ≥1.0×10 8 CFU / ml, the fermentation is completed, and Bacillus subtilis fermentation broth, Lactobacillus lactic acid fermentation broth and Saccharomyces cerevisiae fermentation broth are obtained respectively.

[0030] Preferably, in step 1-2), the fermentation product in the fermentation tank comprises the following raw materials in percentage by weight:

[0031] Soybean meal hydrolyzate: 5-8wt%;

[0032] Glucose: 1-3 wt%;

[0033] Corn steep liquor: 1-3wt%;

[0034] Disodium hydrogen phosphate: 0.1-0.5wt%;

[0035] Manganese sulfate or acid manganese phosphate: 0.1-0.5wt%;

[0036] Magnesium sulfate: 0.01-0.05wt%;

[0037] Zinc sulfate: 0.01-0.05wt%;

[0038] Copper sulfate: 0.01-0.05wt%;

[0039] The balance is water.

[0040] Preferably, in step 1-3), the fermentation product in the production tank comprises the following raw materials in percentage by weight:

[0041] Soybean meal hydrolyzate: 10-12wt%;

[0042] Glucose: 3-5wt%;

[0043] Corn steep liquor: 3-5wt%;

[0044] Disodium hydrogen phosphate: 0.1-0.5wt%;

[0045] Manganese sulfate or acid manganese phosphate: 0.1-0.5wt%;

[0046] Magnesium sulfate: 0.01-0.05wt%;

[0047] Zinc sulfate: 0.01-0.05wt%;

[0048] Copper sulfate: 0.01-0.05wt%;

[0049] The balance is water.

[0050] Preferably, in step 3), the traditional Chinese medicine composition is crushed to 300-500 meshes.

[0051] Preferably, in step 4), the fermentation raw materials contain at least 100 parts by weight of the traditional Chinese medicine composition and 8 to 12 parts by weight of the composite bacteria fermentation liquid.

[0052] More preferably, in step 4), the fermentation raw material comprises the following components in parts by weight:

[0053] 100 portions of crushed Chinese medicine composition,

[0054] 8 to 12 parts of compound bacterial fermentation liquid,

[0055] 20-25 portions of wheat bran,

[0056] 5-10 portions of soybean meal,

[0057] 2.5-8.0 parts calcium carbonate,

[0058] 0.4-0.6 parts tryptophan

[0059] 0.1-0.5 parts of ferrous sulfate,

[0060] 0.1-0.3 parts of zinc sulfate,

[0061] 0.05-0.1 parts of manganese sulfate or acid manganese phosphate,

[0062] 0.05-0.1 parts of magnesium sulfate,

[0063] 0.05-0.1 parts of copper sulfate,

[0064] 0.01-0.05 parts of disodium hydrogen phosphate.

[0065] The probiotics, vitamins and Chinese medicine composition are synergistically compounded to further improve the effect of the present invention on preventing and treating mild cases of nutritional limb and hoof diseases and bacterial limb and hoof diseases.

[0066] Preferably, in step 4), the fermentation raw material is added with water to have a water content of 50-60%, the pH value is adjusted to 5.5-7.0, the fermentation temperature is 25-30° C., and the fermentation time is 96-120 h;

[0067] Stir at least twice every 24 hours, each time for 8 to 10 minutes. When the total number of bacteria reaches ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0068] The present invention also discloses a pig feed, comprising the above additive, wherein the additive is added in an amount of 1 to 3 kg per ton of pig feed.

[0069] The invention also discloses application of the additive for preventing and treating pig limb and hoof diseases in preventing and treating nutritional limb and hoof diseases and bacterial limb and hoof diseases. DETAILED DESCRIPTION

[0070] The technical solutions in the embodiments of the present invention will be described clearly and completely below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0071] Unless otherwise specified, the following materials are conventional commercially available materials.

[0072] Example 1

[0073] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0074] Inoculate Lactobacillus lactis (strain number CGMCC 1.1880) into MRS medium;

[0075] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0076] The shaker was set at 30°C and 120 rpm / min for culture and resurrection until the number of viable cells was ≥ 0.8 × 10 8 CFU / ml, as the primary seed liquid.

[0077] 1-2) Transfer each primary seed solution to a fermentation tank with an inoculation amount of 5% of the total mass of the fermentation product, culture temperature of 25°C, stirring speed of 200 rpm, and ferment until the bacterial content is ≥ 0.8×10 8 CFU / ml, used as secondary seed solution.

[0078] The fermentation in the fermenter contains the following qualities of raw materials:

[0079] Soybean meal hydrolyzate: 5kg (Hubei Xinrunde Chemical Co., Ltd.);

[0080] Glucose: 1kg;

[0081] Corn steep liquor: 1kg;

[0082] Disodium hydrogen phosphate: 0.1kg;

[0083] Manganese sulfate / acid manganese phosphate: 0.1kg;

[0084] Magnesium sulfate: 0.01kg;

[0085] Zinc sulfate: 0.01kg;

[0086] Copper sulfate: 0.01kg;

[0087] Make up to 100 kg with water.

[0088] 1-3) Transfer each secondary seed solution to the production tank, inoculate 10% of the total mass of the fermentation product, culture temperature 25°C, stirring speed 120 rpm, and ferment until the bacterial content is ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactic acid fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0089] The fermentation in the production tank contains the following raw materials of quality:

[0090] Soybean meal hydrolyzate: 10kg (Hubei Xinrunde Chemical Co., Ltd.);

[0091] Glucose: 3kg;

[0092] Corn steep liquor: 3kg;

[0093] Disodium hydrogen phosphate: 0.1kg;

[0094] Manganese sulfate: 0.1kg;

[0095] Magnesium sulfate: 0.01kg;

[0096] Zinc sulfate: 0.01kg;

[0097] Copper sulfate: 0.01kg;

[0098] Make up to 100 kg with water.

[0099] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid are mixed in a weight ratio of 0.8:1.0:0.8 to prepare a composite bacterial fermentation liquid.

[0100] 3) Prepare the following Chinese medicine composition by weight, comprising:

[0101] 55kg of Psoralea corylifolia, 45kg of Atractylodes macrocephala, 45kg of Poria cocos, 35kg of Frankincense, 35kg of Curcuma aromatica, 35kg of Rehmannia root, 20kg of Dipsacus asper, 20kg of Alisma orientalis, 20kg of White Peony Root, 20kg of Myrrha and 20kg of Licorice.

[0102] The above Chinese medicine composition is ultra-finely ground to 300 meshes.

[0103] 4) Prepare the following fermentation raw materials by weight:

[0104] 10kg of crushed Chinese medicine composition,

[0105] 0.8kg compound bacterial fermentation liquid,

[0106] 2kg wheat bran,

[0107] 0.5kg soybean meal,

[0108] 0.25kg calcium carbonate,

[0109] 40g tryptophan,

[0110] 10g ferrous sulfate,

[0111] 10g zinc sulfate,

[0112] 5g manganese sulfate,

[0113] 5g magnesium sulfate,

[0114] 5g copper sulfate,

[0115] 1g disodium hydrogen phosphate.

[0116] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 50%, the pH value was adjusted to 5.5, the fermentation temperature was controlled at 25-30°C, and the fermentation time was 96 hours; the fermentation was turned over and stirred twice every 24 hours, each time for 8 minutes, and the total number of bacteria reached ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0117] 5) Filling into a specification of 25kg / barrel and storing in a ventilated and cool place to obtain an additive for preventing sow limb and hoof diseases.

[0118] Example 2

[0119] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0120] Inoculate Lactobacillus lactis (strain number CGMCC 1.1880) into MRS medium;

[0121] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0122] The shaking incubator was set at 35°C and 150 rpm / min for resurrection until the number of viable cells was ≥ 0.8 × 10 8 CFU / ml, as the primary seed liquid.

[0123] 1-2) Transfer each primary seed solution to a fermentation tank with an inoculation amount of 6% of the total mass of the fermentation product, culture temperature of 28°C, stirring speed of 225 rpm, and fermentation until the bacterial content is ≥ 0.8×10 8 CFU / ml, used as secondary seed solution.

[0124] The fermentation in the fermenter contains the following qualities of raw materials:

[0125] Soybean meal hydrolyzate: 7kg (Hubei Xinrunde Chemical Co., Ltd.);

[0126] Glucose: 2kg;

[0127] Corn steep liquor: 2kg;

[0128] Disodium hydrogen phosphate: 0.3kg;

[0129] Manganese sulfate / acid manganese phosphate: 0.3kg;

[0130] Magnesium sulfate: 0.03kg;

[0131] Zinc sulfate: 0.03kg;

[0132] Copper sulfate: 0.03kg;

[0133] Make up to 100 kg with water.

[0134] 1-3) Transfer each secondary seed solution to the production tank, inoculate at 12% of the total mass of the fermentation product, culture at 27°C, stir at 150 rpm, and ferment until the bacterial content is ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactic acid fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0135] The fermentation in the production tank contains the following raw materials of quality:

[0136] Soybean meal hydrolyzate: 11kg (Hubei Xinrunde Chemical Co., Ltd.);

[0137] Glucose: 4 kg;

[0138] Corn steep liquor: 4kg;

[0139] Disodium hydrogen phosphate: 0.3kg;

[0140] Acid manganese phosphate: 0.3kg;

[0141] Magnesium sulfate: 0.03kg;

[0142] Zinc sulfate: 0.03kg;

[0143] Copper sulfate: 0.03kg;

[0144] Make up to 100 kg with water.

[0145] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid are mixed in a weight ratio of 0.9:1.1:0.9 to prepare a composite bacterial fermentation liquid.

[0146] 3) Prepare the following Chinese medicine composition by weight, comprising:

[0147] 65kg of Psoralea corylifolia, 50kg of Atractylodes macrocephala, 48kg of Poria cocos, 40kg of Frankincense, 40kg of Curcuma aromatica, 38kg of Rehmannia root, 25kg of Dipsacus asper, 28kg of Alisma orientalis, 25kg of White Peony Root, 25kg of Myrrha and 30kg of Licorice.

[0148] The above Chinese medicine composition is ultra-finely ground to 400 meshes.

[0149] 4) Prepare the following fermentation raw materials by weight:

[0150] 10kg of crushed Chinese medicine composition,

[0151] 1kg compound bacterial fermentation liquid,

[0152] 2.2kg wheat bran,

[0153] 0.8kg soybean meal,

[0154] 0.5kg calcium carbonate,

[0155] 50g tryptophan,

[0156] 30g ferrous sulfate,

[0157] 20g zinc sulfate,

[0158] 8g acid manganese phosphate,

[0159] 8g magnesium sulfate,

[0160] 8g copper sulfate,

[0161] 3g disodium hydrogen phosphate.

[0162] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 55%, the pH value was adjusted to 6.0, the fermentation temperature was controlled at 25-30°C, and the fermentation time was 100 h; the fermentation was stirred 3 times every 24 h, each time for 9 min, and the total number of bacteria reached ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0163] 5) Filling into a specification of 25kg / barrel and storing in a ventilated and cool place to obtain an additive for preventing sow limb and hoof diseases.

[0164] Example 3

[0165] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0166] Inoculate Lactobacillus lactis (strain number CGMCC 1.1880) into MRS medium;

[0167] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0168] The shaking incubator was set at 37°C and 180 rpm / min for culture resuscitation until the number of viable cells was ≥ 0.8 × 10 8 CFU / ml, as the primary seed liquid.

[0169] 1-2) Transfer each primary seed solution to a fermentation tank with an inoculation amount of 8% of the total mass of the fermentation product, a culture temperature of 30°C, a stirring speed of 250 rpm, and ferment until the bacterial content is ≥ 0.8 × 10 8 CFU / ml, used as secondary seed solution.

[0170] The fermentation in the fermenter contains the following qualities of raw materials:

[0171] Soybean meal hydrolyzate: 8kg (Hubei Xinrunde Chemical Co., Ltd.);

[0172] Glucose: 3kg;

[0173] Corn steep liquor: 3kg;

[0174] Disodium hydrogen phosphate: 0.5kg;

[0175] Manganese sulfate: 0.5kg;

[0176] Magnesium sulfate: 0.05kg;

[0177] Zinc sulfate: 0.05kg;

[0178] Copper sulfate: 0.05kg;

[0179] Make up to 100 kg with water.

[0180] 1-3) Transfer each secondary seed solution to the production tank, inoculate it with 15% of the total mass of the fermentation product, culture at 30°C, stir at 180 rpm, and ferment until the bacterial content is ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactic acid fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0181] The fermentation in the production tank contains the following raw materials of quality:

[0182] Soybean meal hydrolyzate: 12kg (Hubei Xinrunde Chemical Co., Ltd.);

[0183] Glucose: 5kg;

[0184] Corn steep liquor: 5kg;

[0185] Disodium hydrogen phosphate: 0.5kg;

[0186] Manganese sulfate: 0.5kg;

[0187] Magnesium sulfate: 0.05kg;

[0188] Zinc sulfate: 0.05kg;

[0189] Copper sulfate: 0.05kg;

[0190] Make up to 100 kg with water.

[0191] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid are mixed in a weight ratio of 1.0:1.2:1.0 to prepare a composite bacterial fermentation liquid.

[0192] 3) Prepare the following Chinese medicine composition by weight, comprising:

[0193] 70kg of Psoralea corylifolia, 53kg of Atractylodes macrocephala, 53kg of Poria cocos, 43kg of frankincense, 43kg of Curcuma aromatica, 43kg of Rehmannia root, 33kg of Dipsacus asper, 33kg of Alisma orientalis, 33kg of White Peony Root, 33kg of Myrrha and 33kg of Licorice.

[0194] The above Chinese medicine composition is ultra-finely ground to 500 meshes.

[0195] 4) Prepare the following fermentation raw materials by weight:

[0196] 10kg of crushed Chinese medicine composition,

[0197] 1.2kg compound bacterial fermentation liquid,

[0198] 2.5kg wheat bran,

[0199] 1kg soybean meal,

[0200] 0.8kg calcium carbonate,

[0201] 60g tryptophan,

[0202] 50g ferrous sulfate,

[0203] 30g zinc sulfate,

[0204] 10g manganese sulfate,

[0205] 10g magnesium sulfate,

[0206] 10g copper sulfate,

[0207] 5g disodium hydrogen phosphate.

[0208] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 60%, the pH value was adjusted to 7.0, the fermentation temperature was controlled at 25-30°C, and the fermentation time was 120h; the fermentation was stirred 4 times every 24h, each time for 10min, and the total number of bacteria reached ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0209] 5) Filling into a specification of 25kg / barrel and storing in a ventilated and cool place to obtain an additive for preventing sow limb and hoof diseases.

[0210] Comparative Example 1

[0211] "Super Vita", a commercially available product for the prevention and treatment of limb and hoof diseases.

[0212] Comparative Example 2

[0213] Compared with Example 2, this comparative example comprises the following raw materials by weight:

[0214] 2.2kg wheat bran,

[0215] 0.8kg soybean meal,

[0216] 0.5kg calcium carbonate,

[0217] 50g tryptophan

[0218] 30g ferrous sulfate:

[0219] 20g zinc sulfate:

[0220] 8g manganese sulfate / acid manganese phosphate:

[0221] 8g magnesium sulfate:

[0222] 8g copper sulfate;

[0223] 3g disodium hydrogen phosphate.

[0224] That is, this comparative example does not include a fermented Chinese medicine composition and related production steps.

[0225] (1) In vitro antibacterial test

[0226] Apply to and order Streptococcus suis, Haemophilus parasuis, Erysipelothrix rhizogenes, Staphylococcus aureus and Corynebacterium pyogenes from the National Veterinary Microbial and Virus Collection Center, and inoculate them onto the culture medium.

[0227] Take appropriate amounts of Examples 1 to 3 and Comparative Example 2 for centrifugation, take equal amounts of supernatant after centrifugation and add them to the culture medium of each pathogenic bacteria, culture at 37° C. for 12 h, measure and record the diameter d of the inhibition zone, and the standard is as follows:

[0228] Sensitivity Highly sensitive Moderately sensitive Low sensitivity Insensitive d / mm ＞20 15～20 10～15 ＜10

[0229] The results are as follows:

[0230] Pathogens Streptococcus suis Haemophilus parasuis Erysipelothrix rhizogenes staphylococcus Corynebacterium pyogenes Example 1 Moderately sensitive Moderately sensitive Low sensitivity Highly sensitive Highly sensitive Example 2 Highly sensitive Highly sensitive Moderately sensitive Highly sensitive Highly sensitive Example 3 Highly sensitive Moderately sensitive Moderately sensitive Highly sensitive Highly sensitive Comparative Example 2 Insensitive Insensitive Insensitive Insensitive Insensitive

[0231] The two types of experimental pigs involved in the following test projects were provided by Guangzhou Yangcheng Pig Industry Co., Ltd.

[0232] (2) Sixty sows in early pregnancy were randomly divided into six groups, with 10 sows in each group. The sows were in similar body condition and had healthy limbs and hooves. The groups were numbered EG1-EG3 and CG1-CG3.

[0233] Groups EG1 to EG3 respectively added Examples 1 to 3 to the feed, with a ratio of 2 kg per ton of feed;

[0234] Groups CG1 and CG2 added comparative example 1 and comparative example 2 to the feed, respectively, at a ratio of 2 kg per ton of feed;

[0235] There was no additive in the feed of group CG3.

[0236] The trial period was three months. The initial body weight, back fat thickness and hoof keratin hardness of each test pig were measured when they were enrolled, and then measured and recorded again on the 1st and 15th of each month. The food intake of each test pig was recorded every day for three months and the occurrence of limb and hoof diseases was observed.

[0237] All experimental pigs in the experimental period were fed three times a day in a "feeding + free access to food + drinking water" mode. The breeding site should be kept clean and well ventilated.

[0238] (2-1) Back fat measurement and results

[0239] The initial backfat thickness of each test pig was measured by taking the P2 point on the test pigs using a backfat meter, and then the backfat thickness was measured and recorded again on the 1st and 15th of each month for 3 months.

[0240] If the final backfat thickness of each test pig fluctuates within ±1 mm relative to the initial backfat thickness, it is considered that there is no significant change in the backfat thickness. If it exceeds this fluctuation range, it is considered that the backfat thickness has increased or decreased.

[0241] Group Number of pigs with increased backfat thickness No significant change in backfat thickness Number of pigs with reduced backfat thickness EG1 8 2 0 EG 2 10 0 0 EG 3 9 1 0 CG 1 6 2 2 CG 2 4 3 3 CG 3 2 3 5

[0242] (2-2) Recording and tracking of appetite and weight

[0243] The initial daily food intake of each experimental pig was recorded. If the change in food intake fluctuated between ±0.5kg or the increase in food intake was greater than 0.5kg, it was considered that there was no decrease in appetite, and if it was below this fluctuation range, it was considered that there was a decrease in appetite.

[0244] The initial weight of each test pig was recorded. The weight of the test pigs was measured and recorded before eating on the morning of the 1st and 15th of each month. If the final weight of each test pig increased by at least 10 kg relative to the initial weight, it was considered to be healthy and the nutrient absorption efficiency was normal. Otherwise, it was considered to be abnormal in health and the nutrient absorption efficiency was low.

[0245]

[0246] (2-3) Determination and tracking of pig hoof keratin hardness

[0247] The keratin of the hoof bottom of the test pigs was collected, cleaned, and trimmed into 1 cm pieces of basically uniform size. 2 The hardness of pig hoof keratin was tested using a Leeb hardness tester.

[0248] The data of each measurement were compared with the initial trotter keratin hardness of the test sows. Hardness values ​​within the range of ±1.5 were recorded as no significant change, and hardness values ​​outside this range were recorded as an increase or decrease.

[0249]

[0250] (2-4) Statistics on the number of pigs with limb and hoof disease symptoms during the 3-month trial period

[0251] Group EG 1 EG 2 EG 3 CG 1 CG 2 CG 3 Number of animals with limb and hoof symptoms 1 0 0 2 2 4

[0252] From the above data results, it can be seen that: compared with CG 3 without feed additives, EG1~3 shows that the present invention can effectively reduce the incidence of limb and hoof diseases in pigs; compared with CG 1, it shows that the preventive effect of the present invention on limb and hoof diseases is slightly better than the commercially available product "Super Vita"; compared with CG 2, it shows that the fermented Chinese medicine composition design of the present invention can effectively improve the preventive effect on limb and hoof diseases, which is the key design of the present invention.

[0253] (3) 20 farmed pigs with early symptoms of limb and hoof disease, specifically redness, swelling or slight erosion of the limbs and hooves, accompanied by loss of appetite and / or slight diarrhea (i.e., generally indicating bacterial limb and hoof disease), were randomly divided into 2 groups, 10 in each group, group numbers SP-EG1 and SP-CG1, and Example 2 and Comparative Example 2 were added to the feed, respectively, at a ratio of 2 kg per ton of feed.

[0254] The trial period is two weeks, and the condition is observed to see if it improves or heals within two weeks. All experimental pigs in the trial period are fed three times a day in the "feeding + free access to food + drinking water" mode. The breeding site should be kept clean and well ventilated.

[0255] Group SP-EG1 SP-CG1 Number of animals with improved limb and hoof symptoms 10 6

[0256] The data of Comparative Example 2 show that the scientific and reasonable configuration of nutrients such as vitamins can improve the symptoms of limb and hoof disease in pigs by enhancing the immunity of pigs. However, the improvement of limb and hoof disease in pigs by adding the feed of Comparative Example 2 is not as good as that by adding the feed of Example 2. This shows that the fermented Chinese medicine composition designed by the present invention can effectively improve the therapeutic effect of the additive on mild limb and hoof disease.

[0257] According to the disclosure and teaching of the above description, those skilled in the art to which the present invention belongs may also change and modify the above implementation modes. Therefore, the present invention is not limited to the specific implementation modes disclosed and described above, and some modifications and changes to the present invention should also fall within the protection scope of the claims of the present invention. In addition, although some specific terms are used in this specification, these terms are only for the convenience of description and do not constitute any limitation to the present invention.

Claims

1. An additive for preventing and treating pig limb and hoof diseases, characterized in that: The preparation raw materials at least include composite bacterial fermentation liquid and a traditional Chinese medicine composition; The composite bacterial fermentation liquid comprises Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid; The traditional Chinese medicine composition at least comprises psoralea corylifolia, atractylodes macrocephala, tuckahoe, frankincense, curcuma and raw rehmannia root.

2. The additive for preventing and treating pig limb and hoof disease according to claim 1, characterized in that: The weight ratio of the bacillus subtilis fermentation liquid, the lactobacillus lactic acid fermentation liquid and the saccharomyces cerevisiae fermentation liquid is 0.8-1.0:1.0-1.2:0.8-1.

0.

3. The additive for preventing and treating pig limb and hoof disease according to claim 1 or 2, characterized in that: The Chinese medicine composition at least comprises the following raw materials in parts by weight: 40-70 parts of Psoralea corylifolia, 40-60 parts of Atractylodes macrocephala, 40-60 parts of Poria cocos, 30-50 parts of frankincense, 30-50 parts of Curcuma aromatica and 30-50 parts of Rehmannia root.

4. The additive for preventing and treating pig limb and hoof disease according to claim 3, characterized in that: The Chinese medicine composition comprises the following raw materials in parts by weight: 55-70 parts of Psoralea corylifolia, 45-53 parts of Atractylodes macrocephala, 45-53 parts of Poria cocos, 35-43 parts of frankincense, 35-43 parts of Curcuma aromatica, 35-43 parts of Rehmannia root, 20-33 parts of Dipsacus asper, 20-33 parts of Alisma orientalis, 20-33 parts of White Peony Root, 20-33 parts of Myrrha and 20-33 parts of Licorice.

5. The method for preparing the additive for preventing and treating pig limb and hoof disease according to any one of claims 1 to 4, characterized in that: The steps include: 1) reviving and culturing Bacillus subtilis, Lactobacillus lactis and Saccharomyces cerevisiae to obtain Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid; 2) mixing Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid according to a preset weight ratio to obtain a composite bacterial fermentation liquid; 3) preparing the Chinese medicine composition according to the preset components and weight proportions, and crushing the mixture; 4) preparing fermentation raw materials and performing fermentation, wherein the fermentation raw materials at least include the composite bacterial fermentation liquid and the traditional Chinese medicine composition; 5) After the fermentation is completed, the product is bottled to obtain an additive for preventing and treating pig limb and hoof diseases.

6. The preparation method according to claim 5, characterized in that: In step 4), the fermentation raw materials contain at least 100 parts by weight of the traditional Chinese medicine composition and 8 to 12 parts by weight of the composite bacteria fermentation liquid.

7. The preparation method according to claim 6, characterized in that: In step 4), the fermentation raw material comprises the following components in parts by weight: 100 parts of crushed Chinese medicine composition, 8-12 parts of composite bacteria fermentation liquid, 20-25 parts of wheat bran, 5-10 parts of soybean meal, 2.5-8.0 parts of calcium carbonate, 0.4-0.6 parts of tryptophan, 0.1-0.5 parts of ferrous sulfate, 0.1-0.3 parts of zinc sulfate, 0.05-0.1 parts of manganese sulfate or acid manganese phosphate, 0.05-0.1 parts of magnesium sulfate, 0.05-0.1 parts of copper sulfate and 0.01-0.05 parts of disodium hydrogen phosphate.

8. The preparation method according to claim 7, characterized in that: In step 4), water is added to the fermentation raw material to make the water content 50-60%, the pH value is adjusted to 5.5-7.0, the fermentation temperature is 25-30° C., and the fermentation time is 96-120 hours; Stir at least twice every 24 hours, each time for 8 to 10 minutes. When the total number of bacteria reaches ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

9. A pig feed, characterized in that: The additive for preventing and treating pig limb and hoof diseases comprises the additive according to any one of claims 1 to 4, wherein the additive is added in an amount of 1 to 3 kg per ton of pig feed.

10. Use of the additive for preventing and treating pig limb and hoof diseases according to any one of claims 1 to 4 in preventing and treating nutritional limb and hoof diseases and bacterial limb and hoof diseases.

Images