A prebiotic feed additive for promoting intestinal health of pigs and preparation method thereof

Abstract

The present invention relates to a prebiotic feed additive for promoting intestinal health of pigs, belonging to the technical field of feed, the prebiotic feed additive of the present invention comprises prebiotic components and biological adjuvants, the prebiotic components comprise oligofructose, oligoisomaltose, inulin and resistant dextrin, the biological adjuvant is a product obtained by co-enzymatic hydrolysis of dandelion and houttuynia cordata by hemicellulase and pectinase, then fermented by Lactobacillus acidophilus and Propionibacterium freudenreichii in sequence, and finally enzymatic hydrolyzed by bromelain and trypsin step by step. The components in the additive are used in combination to have a good effect of promoting intestinal health of pigs, can balance and improve the intestinal microenvironment, improve feed nutrient utilization, enhance immunity, reduce diarrheal diseases, and promote growth and weight gain.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and in particular relates to a prebiotic feed additive for promoting intestinal health of pigs and a preparation method thereof. Background Art

[0002] Traditional feed additives mainly include antibiotics and hormones. Although these additives can improve the production performance of animals to a certain extent, they also have many problems. For example, long-term use of antibiotics will lead to the development of bacterial resistance, affecting the health of animals and food safety; the use of hormones may also cause potential harm to human health. In addition, the use of traditional feed additives may also cause environmental pollution.

[0003] With the rapid development of modern animal husbandry, people have higher and higher requirements for the quality and safety of animal products. The intestines of animals are the main place for digestion and absorption of nutrients, and are also the largest immune organ in the animal body. Intestinal health has a vital impact on the growth, development, production performance and immunity of animals. In recent years, with the continuous deepening of research, people have gradually realized the positive effects of substances such as prebiotics on animal intestinal health. Prebiotics can improve the intestinal health of animals and improve their production performance and immunity by regulating the balance of intestinal flora, promoting the growth of beneficial bacteria, and enhancing intestinal barrier function.

[0004] At present, the development of biotechnology provides technical support for the research and development of prebiotic feed additives. With the continuous advancement of biotechnology, people have conducted more and more in-depth research on the mechanism of action, screening methods and production processes of prebiotics. At present, a variety of prebiotics have been successfully screened and applied to feed additives. For example, oligofructose, oligogalactose, mannan oligosaccharide, etc. are all common prebiotic feed additives. These prebiotics can be mass-produced by biotechnology, providing support for the widespread application of prebiotic feed additives.

[0005] Although prebiotics are a green and environmentally friendly feed additive that can reduce the use of traditional feed additives such as antibiotics, the existing prebiotic ingredients are relatively simple, with limited effects, and poor compatibility often leads to insignificant improvement effects. Therefore, the effects of promoting intestinal health and enhancing immunity need to be improved. Summary of the invention

[0006] The existing prebiotic ingredients are relatively single, with limited effects, and poor compatibility often leads to the problem of unclear improvement effects. The effects in promoting intestinal health and enhancing immunity need to be improved. The present invention provides a prebiotic feed additive for promoting intestinal health of pigs and a preparation method thereof. The additive contains prebiotics with a specific ingredient ratio, including oligofructose, oligomaltose, inulin and resistant dextrin; and contains biological adjuvants. The combination of these ingredients has a good effect on promoting intestinal health of pigs, can balance and improve the intestinal microenvironment, improve feed nutrient utilization, enhance immunity, reduce diarrhea, and promote growth and weight gain. The specific technical scheme is as follows:

[0007] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 1 to 10 parts of prebiotic components and 0.5 to 3 parts of biological adjuvants, the prebiotic components comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = (5 to 12): (1.5 to 3): (4 to 8): (0.3 to 0.8);

[0008] The biological auxiliary agent is a composite extract of dandelion and houttuynia cordata, which is a product obtained by hydrolyzing dandelion and houttuynia cordata with hemicellulase and pectinase, fermenting with Lactobacillus acidophilus and Propionibacterium freudenreichii in sequence, and finally hydrolyzing with bromelain and trypsin step by step.

[0009] Among the above additives, the preparation method of the biological adjuvant comprises the following steps:

[0010] According to the mass ratio of dandelion: houttuynia cordata = (8-15): (3-6), the whole dandelion plant and the whole houttuynia cordata plant are mixed and crushed to obtain a mixture; the mixture is added into purified water with a mass of 10 to 15 times of the mass of the mixture, the pH is adjusted to 4.5 to 5.0, the temperature is 40°C to 50°C, hemicellulase and pectinase are added, and enzymolysis is carried out for 3h to 4h, then yeast flavor and glucose are added, and after uniform mixing, the pH is adjusted to 5.5 to 6.0, and Lactobacillus acidophilus is added, and anaerobically fermented at 36°C to 38°C for 15h to 20h, and after the temperature drops to 36°C to 38°C, the pH is adjusted to 5.5 to 6.0, and Lactobacillus acidophilus is added, and the mixture is anaerobically fermented at 36°C to 38°C for 15h to 20h. H6.8~7.0, then add potassium dihydrogen phosphate and magnesium sulfate, add Propionibacterium freudenreichii, ferment anaerobicly at constant temperature for 20h~25h, adjust pH6.0~7.5, add bromelain, hydrolyze at 50℃~60℃ for 1h~1.5h, sterilize and inactivate enzyme, reduce to 35℃~40℃, adjust pH7.5~8.5, add trypsin, hydrolyze at constant temperature for 50min~80min, sieve, concentrate, dry, and break up to obtain biological adjuvant.

[0011] In the preparation method of the above-mentioned biological auxiliary agent, the added amount of the hemicellulase is 0.5% to 2% of the mass of the mixture; the added amount of the pectinase is 0.5% to 2% of the mass of the mixture; the added amount of the yeast flavor is 3% to 5% of the mass of the mixture; the added amount of the glucose is 3% to 5% of the mass of the mixture; the added amount of the Lactobacillus acidophilus is 0.5% to 1.5% of the mass of the mixture; the added amount of the potassium dihydrogen phosphate is 0.01% to 0.03% of the mass of the mixture; the added amount of the magnesium sulfate is 0.01% to 0.03% of the mass of the mixture; the added amount of the Propionibacterium freudenreichii is 0.5% to 1.5% of the mass of the mixture; the added amount of the bromelain is 0.5% to 1.5% of the mass of the mixture; and the added amount of the trypsin is 0.5% to 1.5% of the mass of the mixture.

[0012] In the preparation method of the above biological adjuvant, the enzyme activity of the hemicellulase is 100,000 U / g to 200,000 U / g, and the enzyme activity of the pectinase is 30,000 U / g to 50,000 U / g.

[0013] In the preparation method of the above biological adjuvant, the bacterial activity of the Lactobacillus acidophilus is 10 billion CFU / g to 50 billion CFU / g, and the bacterial activity of the Propionibacterium freudenreichii is 2 billion CFU / g to 5 billion CFU / g.

[0014] In the preparation method of the above biological auxiliary agent, the enzymatic activity of the bromelain is 100,000 U / g to 500,000 U / g, and the enzymatic activity of the trypsin is 50,000 U / g to 100,000 U / g.

[0015] In the above-mentioned method for preparing the biological adjuvant, the temperature for sterilization and enzyme inactivation is 80°C to 95°C, and the time is 10min to 20min.

[0016] In the above-mentioned method for preparing the biological adjuvant, the mesh size of the sieving sieve is 100-250 meshes.

[0017] In the above-mentioned method for preparing the biological adjuvant, the concentration pressure is 0.03MPa-0.06MPa, and the temperature is 40°C-50°C.

[0018] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0019] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0020] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0021] The prebiotic feed additive for promoting intestinal health of pigs and the preparation method thereof provided by the present invention have the following beneficial effects compared with the prior art:

[0022] 1. The prebiotic feed additive of the present invention contains prebiotics (fructooligosaccharides, isomaltooligosaccharides, inulin and resistant dextrin) and biological adjuvants (composite extracts of dandelion and houttuynia cordata) with specific ingredient ratios. The combined use of the ingredients has a good effect on promoting the intestinal health of pigs, can balance and improve the intestinal microenvironment, improve the utilization rate of feed nutrients, enhance immunity, reduce diarrhea symptoms, and promote growth and weight gain.

[0023] 2. As a proliferation factor for beneficial bacteria such as bifidobacteria, oligofructose can selectively stimulate the growth and reproduction of beneficial bacteria in the intestine. These beneficial bacteria form a dominant flora in the intestine, inhibit the growth of harmful bacteria, and maintain the balance of intestinal microecology. Beneficial bacteria ferment oligofructose to produce short-chain fatty acids and lower the pH value of the intestine. The acidic environment is not conducive to the growth of harmful bacteria, and at the same time helps promote intestinal peristalsis and reduce the residence time of harmful substances in the intestine. The metabolites of beneficial bacteria can enhance the tight connection of intestinal epithelial cells, reduce intestinal permeability, and prevent harmful substances from entering the body, thereby protecting intestinal health.

[0024] 3. Isomaltooligosaccharide can also be used by beneficial intestinal bacteria to promote their growth. The increase of beneficial bacteria helps maintain the stability of intestinal flora and prevent the invasion of pathogenic bacteria. Some beneficial bacteria will produce antibacterial substances, such as bacteriocins, in the process of using isomaltooligosaccharide, which have an inhibitory effect on harmful bacteria. Isomaltooligosaccharide can promote the secretion of digestive enzymes in the intestine, improve the digestion and absorption rate of feed, reduce the accumulation of undigested substances in the intestine, and reduce the burden on the intestine.

[0025] 4. Inulin can significantly increase the number of beneficial bacteria such as bifidobacteria and reduce the proportion of harmful bacteria. By changing the structure of intestinal flora, it can improve the immunity and disease resistance of the intestine. Inulin is fermented by intestinal flora to produce short-chain fatty acids such as acetic acid, propionic acid, butyric acid, etc. These short-chain fatty acids provide energy for intestinal epithelial cells and promote the growth and repair of intestinal cells. Inulin can absorb water, increase stool volume, stimulate intestinal peristalsis, and prevent the occurrence of intestinal diseases.

[0026] 5. Resistant dextrin can provide nutrition for beneficial intestinal bacteria and promote their growth and reproduction. The increase of beneficial bacteria helps maintain the balance of intestinal microecology and improve intestinal health. Resistant dextrin can delay the digestion and absorption of carbohydrates, prevent metabolic diseases in pigs and improve intestinal health. Resistant dextrin can increase the pig's sense of fullness, reduce feed intake, and avoid the burden on the intestine caused by excessive feeding. At the same time, reasonable feed intake control also helps maintain the normal function of the intestine.

[0027] The use of isomaltooligosaccharide and resistant dextrin in a certain proportion has a good synergistic effect, which can better balance the intestinal microenvironment and greatly promote the intestinal absorption function.

[0028] 6. The biological adjuvant of the present invention is a composite extract of dandelion and houttuynia cordata, which is a product obtained by hydrolyzing dandelion and houttuynia cordata with hemicellulase and pectinase, fermenting them with Lactobacillus acidophilus and Propionibacterium freudenreichii in sequence, and finally hydrolyzing them step by step with bromelain and trypsin.

[0029] Among them, dandelion itself has a certain antibacterial effect. After specific fermentation and enzymatic hydrolysis, some new antibacterial ingredients will be produced. These ingredients can inhibit the growth of harmful bacteria in the intestine, reduce the risk of intestinal infection, help maintain the balance of intestinal flora, promote the growth of beneficial bacteria, inhibit the reproduction of harmful bacteria, and improve intestinal immunity and digestive function by regulating the structure of intestinal flora. Dandelion fermentation enzymatic hydrolysate can enhance the tight connection of intestinal epithelial cells, reduce intestinal permeability, and prevent harmful substances from entering the body. At the same time, it can also promote the secretion of intestinal mucus and further protect the intestinal mucosa. Containing some digestive enzymes or substances that promote digestion can improve the digestibility of feed, reduce the accumulation of undigested substances in the intestine, and reduce the burden on the intestine.

[0030] Among them, Houttuynia cordata has antibacterial and antiviral effects. After specific fermentation and enzymatic hydrolysis, its active ingredients can be enhanced, inhibiting pathogenic bacteria and viruses in the intestines and preventing intestinal infections. Houttuynia cordata fermentation enzymatic hydrolysate helps to remove heat toxins in the intestines and reduce intestinal inflammatory reactions; regulate the immune system of pigs and enhance the immune function of the intestines; produce some beneficial metabolites, such as organic acids, etc., regulate the pH value of the intestines, create an environment conducive to the growth of beneficial bacteria, and at the same time, promote intestinal peristalsis and improve the nutrient absorption capacity of the intestines. DETAILED DESCRIPTION

[0031] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0032] Oligofructose: Shandong Aicai Biotechnology Co., Ltd. Isomaltooligosaccharide: Shandong Xinxiong Biotechnology Co., Ltd. Inulin: Hebei Hongtao Bioengineering Co., Ltd. Resistant dextrin: Guangzhou Huayu Biotechnology Co., Ltd. Yeast flavor: Xi'an Lavia Biotechnology Co., Ltd.

[0033] Example 1

[0034] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 6 parts of prebiotic components and 2.5 parts of biological adjuvants, the prebiotic components comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = 8:2.2:6:0.5;

[0035] The preparation method of the biological adjuvant comprises the following steps:

[0036] The whole dandelion plant and the whole houttuynia cordata plant were mixed and crushed according to the mass ratio of dandelion to houttuynia cordata = 12:5 to obtain a mixture; the mixture was added into purified water 12 times the mass of the mixture, the pH was adjusted to 4.8, the temperature was 45°C, 1.2% of hemicellulase and pectinase were added to the mixture respectively, and enzymolysis was carried out for 3.5 hours, then 4% of yeast flavor and glucose were added to the mixture respectively, and after uniform mixing, the pH was adjusted to 5.8, 1% of Lactobacillus acidophilus was added to the mixture, and anaerobically fermented at 37°C for 18 hours, and after cooling to 37°C, the mixture was adjusted to 45°C. The mixture is adjusted to pH 6.9, and then 0.02% of potassium dihydrogen phosphate and magnesium sulfate are added respectively, 1% of Propionibacterium freudenreichii is added, and the mixture is fermented anaerobicy at a constant temperature for 23 hours, the pH is adjusted to 7.0, 1% of bromelain is added, and the mixture is enzymatically hydrolyzed at 55° C. for 1 hour, sterilized and enzyme-inactivating at 85° C. for 15 minutes, the mixture is cooled to 37° C., the pH is adjusted to 8.0, 1% of trypsin is added, and the mixture is enzymatically hydrolyzed at a constant temperature for 60 minutes, passed through a 200-mesh sieve, concentrated, dried, and dispersed under the conditions of a pressure of 0.05 MPa and a temperature of 45° C. to obtain a biological adjuvant.

[0037] The enzymatic activity of the hemicellulase used in this embodiment is 200,000 U / g, the enzymatic activity of the pectinase is 50,000 U / g, the bacterial activity of Lactobacillus acidophilus is 30 billion CFU / g, the bacterial activity of Propionibacterium freudenreichii is 2 billion CFU / g, the enzymatic activity of bromelain is 100,000 U / g, and the enzymatic activity of trypsin is 50,000 U / g.

[0038] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0039] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0040] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0041] Example 2

[0042] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 1 part of prebiotic ingredients and 0.5 parts of biological adjuvants, the prebiotic ingredients comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = 5:1.5:4:0.3;

[0043] The preparation method of the biological adjuvant comprises the following steps:

[0044] The whole dandelion plant and the whole houttuynia cordata plant were mixed and crushed in a mass ratio of dandelion to houttuynia cordata = 8:3 to obtain a mixture; the mixture was added into purified water 10 times the mass of the mixture, the pH was adjusted to 4.5, the temperature was 40°C, 0.5% of hemicellulase and pectinase were added to the mixture by mass, and enzymolysis was performed for 3 hours, then 3% of yeast flavor and glucose were added to the mixture by mass, and after uniform mixing, the pH was adjusted to 5.5, 0.5% of Lactobacillus acidophilus by mass of the mixture was added, and anaerobically fermented at 36°C for 15 hours. After the temperature dropped to 36°C, the pH was adjusted to 6. 8. Then, 0.01% of potassium dihydrogen phosphate and magnesium sulfate are added to the mixture respectively, 0.5% of Propionibacterium freudenreichii is added to the mixture, and the mixture is fermented anaerobicy at a constant temperature for 20 hours, the pH is adjusted to 6.0, 0.5% of bromelain is added to the mixture, and the mixture is enzymatically hydrolyzed at 50° C. for 1 hour, sterilized and enzyme-inactivating at 80° C. for 10 minutes, the mixture is reduced to 35° C., the pH is adjusted to 7.5, 0.5% of trypsin is added to the mixture, and the mixture is enzymatically hydrolyzed at a constant temperature for 50 minutes, the mixture is passed through a 100-mesh sieve, and the mixture is concentrated, dried, and dispersed at a pressure of 0.03 MPa and a temperature of 40° C. to obtain a biological adjuvant.

[0045] The enzymatic activity of the hemicellulase used in this embodiment is 100,000 U / g, the enzymatic activity of the pectinase is 30,000 U / g, the bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g, the bacterial activity of Propionibacterium freudenreichii is 2 billion CFU / g, the enzymatic activity of bromelain is 100,000 U / g, and the enzymatic activity of trypsin is 50,000 U / g.

[0046] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0047] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0048] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0049] Example 3

[0050] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 1 part of prebiotic ingredients and 3 parts of biological adjuvants, the prebiotic ingredients comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = 5:3:4:0.8;

[0051] The preparation method of the biological adjuvant comprises the following steps:

[0052] The whole dandelion plant and the whole houttuynia cordata plant were mixed and crushed according to the mass ratio of dandelion to houttuynia cordata = 8:4.5 to obtain a mixture; the mixture was added into purified water 10 times the mass of the mixture, the pH was adjusted to 5.0, the temperature was 40°C, 2% of hemicellulase and pectinase were added to the mixture by mass, and enzymolysis was performed for 3 hours, then 5% of yeast flavor and glucose were added to the mixture by mass, and after uniform mixing, the pH was adjusted to 5.5, 1.5% of Lactobacillus acidophilus was added to the mixture by mass, and anaerobically fermented at 36°C for 20 hours. After the temperature dropped to 36°C, the pH was adjusted to 7. 0, then add 0.01% of potassium dihydrogen phosphate and magnesium sulfate in the mixture respectively, add 1.5% of Propionibacterium freudenreichii in the mixture, ferment at constant temperature for 20 hours, adjust the pH to 7.5, add 0.5% of bromelain in the mixture, hydrolyze at 60°C for 1 hour, sterilize and inactivate the enzyme at 95°C for 10 minutes, reduce the temperature to 40°C, adjust the pH to 7.5, add 1.5% of trypsin in the mixture, hydrolyze at constant temperature for 50 minutes, pass through a 250-mesh sieve, concentrate at a pressure of 0.03 MPa and a temperature of 50°C, dry, and break up to obtain a biological adjuvant.

[0053] The enzymatic activity of the hemicellulase used in this embodiment is 100,000 U / g, the enzymatic activity of the pectinase is 50,000 U / g, the bacterial activity of Lactobacillus acidophilus is 10 billion CFU / g, the bacterial activity of Propionibacterium freudenreichii is 5 billion CFU / g, the enzymatic activity of bromelain is 100,000 U / g, and the enzymatic activity of trypsin is 100,000 U / g.

[0054] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0055] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0056] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0057] Example 4

[0058] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 10 parts of prebiotic components and 3 parts of biological adjuvants, the prebiotic components comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = 12:3:8:0.8;

[0059] The preparation method of the biological adjuvant comprises the following steps:

[0060] The whole dandelion plant and the whole houttuynia cordata plant were mixed and crushed according to the mass ratio of dandelion to houttuynia cordata = 15:6 to obtain a mixture; the mixture was added into purified water 15 times the mass of the mixture, the pH was adjusted to 5.0, the temperature was 50°C, 2% of hemicellulase and pectinase were added into the mixture by mass, and enzymolysis was performed for 4 hours, then 5% of yeast flavor and glucose were added into the mixture by mass, and the mixture was mixed evenly, and the pH was adjusted to 6.0, and 1.5% of Lactobacillus acidophilus was added into the mixture by mass, and the mixture was anaerobically fermented at 38°C for 20 hours. After the temperature dropped to 38°C, the pH was adjusted to 7.0 , then add 0.03% of potassium dihydrogen phosphate and magnesium sulfate to the mixture respectively, add 1.5% of Propionibacterium freudenreichii to the mixture, ferment anaerobicly at constant temperature for 25 hours, adjust the pH to 7.5, add 1.5% of bromelain to the mixture, enzymolysis at 60°C for 1.5 hours, sterilize and inactivate the enzyme at 95°C for 20 minutes, reduce the temperature to 40°C, adjust the pH to 8.5, add 1.5% of trypsin to the mixture, enzymolysis at constant temperature for 80 minutes, pass through a 250-mesh sieve, concentrate, dry and break up at a pressure of 0.06 MPa and a temperature of 50°C to obtain a biological adjuvant.

[0061] The enzymatic activity of the hemicellulase used in this embodiment is 200,000 U / g, the enzymatic activity of the pectinase is 50,000 U / g, the bacterial activity of Lactobacillus acidophilus is 50 billion CFU / g, the bacterial activity of Propionibacterium freudenreichii is 5 billion CFU / g, the enzymatic activity of bromelain is 500,000 U / g, and the enzymatic activity of trypsin is 100,000 U / g.

[0062] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0063] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0064] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0065] Example 5

[0066] A prebiotic feed additive for promoting intestinal health of pigs, the additive comprising 10 parts of prebiotic components and 0.5 parts of biological adjuvants, the prebiotic components comprising oligofructose, oligomaltose, inulin and resistant dextrin, the mass ratio of which is: oligofructose: oligomaltose: inulin: resistant dextrin = 12:1.5:8:0.3;

[0067] The preparation method of the biological adjuvant comprises the following steps:

[0068] The whole dandelion plant and the whole houttuynia cordata plant were mixed and crushed according to the mass ratio of dandelion to houttuynia cordata = 15:3 to obtain a mixture; the mixture was added into purified water 15 times the mass of the mixture, the pH was adjusted to 4.5, the temperature was 50°C, 0.5% of hemicellulase and pectinase were added to the mixture respectively, and enzymolysis was carried out for 4 hours, and then 3% of yeast flavor and glucose were added to the mixture respectively, and after mixing evenly, the pH was adjusted to 4.5 and the temperature was adjusted to 50°C. H6.0, add 0.5% of Lactobacillus acidophilus by weight of the mixture, ferment anaerobically at 38°C for 15h, after reducing to 38°C, adjust the pH to 6.8, then add 0.03% of potassium dihydrogen phosphate and magnesium sulfate by weight of the mixture respectively, add 0.5% of Propionibacterium freudenreichi by weight of the mixture, ferment anaerobically at constant temperature for 25h, adjust the pH to 6.0, add 1.5% of bromelain by weight of the mixture, hydrolyze at 50°C for 1.5h, sterilize and inactivate enzyme at 80°C for 20min, reduce to 35°C, adjust the pH to 8.5, add 0.5% of trypsin by weight of the mixture, hydrolyze at constant temperature for 80min, pass through a 100-mesh sieve, concentrate, dry and disperse under the conditions of pressure of 0.06MPa and temperature of 40°C to obtain a biological adjuvant.

[0069] The enzymatic activity of the hemicellulase used in this embodiment is 200,000 U / g, the enzymatic activity of the pectinase is 30,000 U / g, the bacterial activity of Lactobacillus acidophilus is 50 billion CFU / g, the bacterial activity of Propionibacterium freudenreichii is 2 billion CFU / g, the enzymatic activity of bromelain is 500,000 U / g, and the enzymatic activity of trypsin is 50,000 U / g.

[0070] The preparation method of the above-mentioned prebiotic feed additive for promoting intestinal health of pigs comprises the following steps:

[0071] S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component;

[0072] S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

[0073] Comparative Example 1

[0074] In the prebiotic ingredients, isomaltooligosaccharide was not added, and the content of isomaltooligosaccharide was replaced by oligofructose; other parameters and methods were the same as those in Example 1.

[0075] Comparative Example 2

[0076] In the prebiotic ingredients, no resistant dextrin is added, and the content of resistant dextrin is replaced by oligofructose; other parameters and methods are the same as in Example 1.

[0077] Comparative Example 3

[0078] In the prebiotic ingredients, isomaltooligosaccharide and resistant dextrin are not added, and the contents of isomaltooligosaccharide and resistant dextrin are replaced by oligofructose; other parameters and methods are the same as in Example 1.

[0079] Comparative Example 4

[0080] In the prebiotic component, the ratio of isomaltooligosaccharide and resistant dextrin is interchanged, that is, the mass ratio is: fructooligosaccharide: isomaltooligosaccharide: inulin: resistant dextrin = 8:0.5:6:2.2; other parameters and methods are the same as in Example 1.

[0081] Comparative Example 5

[0082] In the preparation method of the biological adjuvant, Houttuynia cordata was not added; other parameters and methods were the same as in Example 1.

[0083] Comparative Example 6

[0084] In the preparation method of the biological adjuvant, no dandelion is added; other parameters and methods are the same as in Example 1.

[0085] Comparative Example 7

[0086] In the preparation method of the biological adjuvant, the whole plant of dandelion and the whole plant of houttuynia are mixed and crushed according to the mass ratio of dandelion: houttuynia=12:5 to obtain a mixture; the mixture is added to purified water 12 times the mass of the mixture, the pH is adjusted to 4.8, the temperature is 45°C, and 1.2% of the mass of the mixture is added to the hemicellulase and pectinase, and enzymolysis is performed for 3.5 hours, and then 4% of the mass of the mixture is added to the yeast flavor and glucose, and after mixing evenly, the pH is adjusted to 5.8, 1% of the mass of the mixture is added to the Lactobacillus acidophilus, and anaerobically fermented at 37°C for 18 hours, the pH is adjusted to 8.0, and 1% of the mass of the mixture is added to the trypsin, and enzymolysis is performed at 37°C for 60 minutes, and the mixture is passed through a 200 mesh sieve, and concentrated, dried, and broken up under the conditions of pressure 0.05MPa and temperature 45°C to obtain the biological adjuvant. Other parameters and methods are the same as in Example 1.

[0087] Comparative Example 8

[0088] In the preparation method of the biological adjuvant, the whole plant of dandelion and the whole plant of houttuynia are mixed and crushed according to the mass ratio of dandelion: houttuynia=12:5 to obtain a mixture; the mixture is added to purified water 12 times the mass of the mixture, the pH is adjusted to 4.8, the temperature is 45°C, and 1.2% of the mass of the mixture is added to the hemicellulase and pectinase, and enzymolysis is performed for 3.5 hours, and then 4% of the mass of the mixture is added to the yeast flavor and glucose, and 0.02% of the mass of the mixture is added to the potassium dihydrogen phosphate and magnesium sulfate, and after mixing evenly, the pH is adjusted to 6.9, 1% of the mass of the mixture is added to the Propionibacterium freudenreich, and the mixture is anaerobic fermented at constant temperature for 23 hours, the pH is adjusted to 7.0, 1% of the mass of the mixture is added to the bromelain, and the mixture is enzymolyzed at 55°C for 1 hour, passed through a 200 mesh sieve, concentrated at a pressure of 0.05M Pa and a temperature of 45°C, dried, and broken up to obtain a biological adjuvant. Other parameters and methods are the same as in Example 1.

[0089] Experiment 1: Feed digestibility experiment:

[0090] Raw feed: 65% corn meal, 18% soybean meal, 15% wheat bran meal, 2% fish meal.

[0091] Test pigs: 5-month-old pigs from a pig farm, each pig was raised in fences.

[0092] A number of test pigs were selected and divided into 13 groups, with 10 pigs in each group (each group corresponds to the additives of each embodiment and each comparative example).

[0093] Pre-test test: feed the original feed, and record the daily feed intake of each pig for one week (calculated by weighing the amount of feed fed each day and the amount of remaining feed); collect feces, weigh the collected feces, and analyze the nutritional components of the fed feed and the collected feces respectively. The crude protein content is determined by the Kjeldahl method, and the crude fat content is determined by the Soxhlet extraction method; the feed digestibility is calculated based on the nutritional content of feed intake and feces excretion.

[0094] Experimental detection: The additives of each embodiment and each comparative example were added to the original feed of each group of test pigs, and the additive addition ratio was 0.5wt% of the pig feed. The daily feed intake of each pig was recorded for one week (calculated by weighing the amount of feed fed and the amount of remaining feed per day); and feces were collected and weighed, and the nutrient components of the fed feed and the collected feces were analyzed respectively, the crude protein content was determined by the Kjeldahl method, and the crude fat content was determined by the Soxhlet extraction method; the feed digestibility was calculated based on the nutrient content of the feed intake and the feces excreted.

[0095] Crude protein digestibility = (amount of crude protein ingested - amount of crude protein excreted in feces) / amount of crude protein ingested × 100%. Crude fat digestibility = (amount of crude fat ingested - amount of crude fat excreted in feces) / amount of crude fat ingested × 100%. The test results are shown in Table 1 below.

[0096] Table 1 Feed digestibility (average value)

[0097]

[0098] It can be seen from the above results that the additives of Examples 1 to 5 can greatly improve the digestibility of feed, and the compound prebiotics and biological adjuvants can well promote the intestinal absorption function, balance the intestinal microenvironment, and improve the utilization rate of feed nutrition. From the results of Comparative Examples 1 and 2, it can be seen that not adding oligosaccharides or resistant dextrins will affect the decline in feed digestibility, and from the results of Comparative Example 3, not adding oligosaccharides and resistant dextrins will affect the decline in feed digestibility. From the results of Comparative Examples 1 to 4, it can be seen that oligosaccharides and resistant dextrins both have the effect of improving the intestinal absorption function, thereby improving the digestibility of feed, and oligosaccharides and resistant dextrins are used in a certain proportion, which can greatly improve the digestibility of feed. If the ratio of oligosaccharides and resistant dextrins is exchanged, it will greatly affect the intestinal microenvironment and greatly reduce the digestibility of feed, which shows that oligosaccharides and resistant dextrins are used in a certain proportion with good synergistic effect, which can better balance the intestinal microenvironment and greatly promote the absorption function of the intestine. From the results of Comparative Examples 5 and 6, it can be seen that the fermented hydrolysates of Houttuynia cordata and Taraxacum officinale respectively have good effects of balancing the intestinal microenvironment, promoting intestinal absorption function, and improving feed nutrient utilization. From the results of Comparative Examples 7 and 8, it can be seen that changing the preparation method of the biological adjuvant and obtaining different product components will affect the efficacy of the biological adjuvant and significantly reduce it.

[0099] Experiment 2: Detection of the ratio of some beneficial bacteria to harmful bacteria:

[0100] According to the steps of Experiment 1, the pig feces on the 7th day were obtained, the feces samples were placed in sterile containers, and tested as soon as possible. The beneficial bacteria: lactic acid bacteria and bifidobacteria; harmful bacteria: Escherichia coli and Salmonella were cultured using selective culture media.

[0101] The cultured bacteria were counted using the plate counting method to calculate the number of beneficial bacteria and harmful bacteria. Calculation ratio: ratio of beneficial bacteria to harmful bacteria = number of beneficial bacteria (total number of lactic acid bacteria and bifidobacteria) / number of harmful bacteria (total number of Escherichia coli and Salmonella), that is, the multiple of beneficial bacteria relative to harmful bacteria, referred to as the beneficial bacteria multiple. The test results are shown in Table 2 below.

[0102] Table 2 Proportion of some beneficial bacteria and harmful bacteria (average value)

[0103]

[0104] As can be seen from the above results, the additives of Examples 1 to 5 can increase the number of beneficial bacteria and better balance the intestinal microenvironment. As can be seen from the results of Comparative Examples 1 and 2, adding isomaltooligosaccharide or resistant dextrin can promote the reproduction of beneficial bacteria and balance the intestinal microenvironment. As can be seen from the results of Comparative Example 3, adding isomaltooligosaccharide and resistant dextrin at the same time can promote the reproduction of beneficial bacteria and balance the intestinal microenvironment. As can be seen from the results of Comparative Examples 3 to 4, both isomaltooligosaccharide and resistant dextrin have the effect of promoting the reproduction of beneficial bacteria and improving the intestinal microenvironment. If the ratio of isomaltooligosaccharide and resistant dextrin is exchanged, the intestinal microenvironment will be affected and the improvement effect will be reduced, thus illustrating that isomaltooligosaccharide and resistant dextrin are used in a certain proportion to have a good synergistic effect of coordinating the intestinal microenvironment. From the results of Comparative Examples 5 and 6, it can be seen that the fermentation hydrolysates of Houttuynia cordata and Taraxacum officinale respectively have good functions of balancing the intestinal microenvironment; from the results of Comparative Examples 7 and 8, it can be seen that changing the preparation method of the biological adjuvant and obtaining different product components will affect the improvement effect of the biological adjuvant on the intestinal environment.

[0105] Experiment 3: Diarrhea rate detection:

[0106] Raw feed: 65% corn meal, 18% soybean meal, 15% wheat bran meal, 2% fish meal.

[0107] Test pigs: 4-month-old pigs from a pig farm, each pig was raised in fences.

[0108] 84 test pigs were selected and weighed; they were divided into 14 groups, with 6 pigs in each group, 13 of which corresponded to the additives of each embodiment and each comparative example, and the other group was a blank control group.

[0109] The blank control group was fed with the original feed (without additives), and the feeds of the other groups were added with the additives of the embodiments and comparative examples, respectively, with the additive addition ratio being 0.5% of the pig feed.

[0110] Feed the pigs continuously for 2 months, observe the diarrhea of ​​the pigs every day, record the total number of fecal excretions and total diarrhea times of each group, and calculate the diarrhea rate; weigh each pig after 2 months; record the average weight of each group of pigs before and after the test, and calculate the average weight gain rate.

[0111] Diarrhea rate = total diarrhea times / total feces excretion times × 100%. Average weight gain rate = (average weight of pig group after test - average weight of pig group before test) / total weight of pig group before test × 100%. The test results are shown in Table 3 below.

[0112] Table 3 Diarrhea rate and average weight gain rate

[0113]

[0114]

[0115] It can be seen from the above results that the additives of Examples 1 to 5 can improve intestinal health, enhance immunity, reduce diarrhea, promote intestinal absorption of nutrients, and then promote growth and increase weight. From the results of Comparative Examples 1 to 4, it can be seen that oligosaccharides and resistant dextrins used in a certain proportion have a good synergistic effect of harmonizing the intestinal microenvironment, enhancing immunity, reducing diarrhea, and promoting intestinal absorption of nutrients, thereby promoting growth and increasing weight. From the results of Comparative Examples 5 and 6, it can be seen that Houttuynia cordata and dandelion fermentation hydrolysates have the effects of improving the intestinal microenvironment, enhancing immunity, reducing diarrhea, promoting intestinal absorption of nutrients, and assisting in promoting growth and weight gain; from the results of Comparative Examples 7 and 8, it can be seen that changing the preparation method of the biological adjuvant and obtaining different product components will affect the decline in the effect of the biological adjuvant on improving intestinal health.

Claims

1. A prebiotic feed additive for promoting intestinal health of pigs, characterized in that: The additives include 1 to 10 parts of prebiotic ingredients and 0.5 to 3 parts of biological adjuvants, wherein the prebiotic ingredients include oligofructose, oligomaltose, inulin and resistant dextrin, and the mass ratio is: oligofructose: oligomaltose: inulin: resistant dextrin = (5 to 12): (1.5 to 3): (4 to 8): (0.3 to 0.8); The preparation method of the biological adjuvant comprises the following steps: mixing the whole dandelion plant and the whole houttuynia cordata plant according to the mass ratio of dandelion: houttuynia cordata = (8-15): (3-6), and crushing to obtain a mixture; adding the mixture into purified water 10 to 15 times the mass of the mixture, adjusting the pH to 4.5 to 5.0, the temperature to 40°C to 50°C, adding 0.5% to 2% of hemicellulase by mass of the mixture and 0.5% to 2% of pectinase by mass of the mixture, performing enzymolysis for 3h to 4h, then adding 3% to 5% of yeast flavor by mass of the mixture and 3% to 5% of glucose by mass of the mixture, mixing evenly, adjusting the pH to 5.5 to 6.0, adding 0.5% to 1.5% of Lactobacillus acidophilus by mass of the mixture, and performing anaerobic fermentation at 36°C to 38°C. The temperature was adjusted to 36-38°C for 15-20 hours, and then the pH value was adjusted to 6.8-7.

0. Then, 0.01%-0.03% of potassium dihydrogen phosphate and 0.01%-0.03% of magnesium sulfate were added, and 0.5%-1.5% of Propionibacterium freudenreichii were added. The mixture was anaerobic fermented at a constant temperature for 20-25 hours. The pH value was adjusted to 6.0-7.

5. Bromelain was added at a mass of 0.5%-1.5% and the mixture was enzymolyzed at 50-60°C for 1-1.5 hours. The mixture was sterilized and the enzyme was inactivated. The temperature was adjusted to 35-40°C, the pH value was adjusted to 7.5-8.5, and trypsin was added at a mass of 0.5%-1.5% and the mixture was enzymolyzed at a constant temperature for 50-80 minutes. The mixture was sieved, concentrated, dried, and broken up to obtain a biological adjuvant.

2. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The enzymatic activity of the hemicellulase is 100,000 U / g to 200,000 U / g, and the enzymatic activity of the pectinase is 30,000 U / g to 50,000 U / g.

3. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The bacterial activity of the Lactobacillus acidophilus is 10 billion CFU / g to 50 billion CFU / g, and the bacterial activity of the Propionibacterium freudenreichii is 2 billion CFU / g to 5 billion CFU / g.

4. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The enzymatic activity of the bromelain is 100,000 U / g to 500,000 U / g, and the enzymatic activity of the trypsin is 50,000 U / g to 100,000 U / g.

5. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The sterilization and enzyme inactivation process is carried out at a temperature of 80°C to 95°C and for a time of 10 to 20 minutes.

6. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The mesh number of the sieving screen is 100-250 meshes.

7. A prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The concentration pressure is 0.03MPa-0.06MPa, and the temperature is 40°C-50°C.

8. The method for preparing a prebiotic feed additive for promoting intestinal health of pigs according to claim 1, characterized in that: The preparation method comprises the following steps: S1: mixing oligofructose, oligomaltose, inulin and resistant dextrin according to mass ratio to obtain a prebiotic component; S2: adding biological adjuvants to the prebiotic components, and mixing them to obtain additives.

Images