Method for improving implantation rate of frozen-thawed embryo transplantation of RIF patient

Abstract

The invention relates to the technical field of embryo transplantation, in particular to a method for improving the implantation rate of RI F patient freeze-thaw embryo transplantation, which comprises the following steps: S100: selecting a patient diagnosed as RI F, and carrying out comprehensive reproductive health assessment, including detection of indexes such as endometrial thickness and hormone level; s200: ensuring that the patient conforms to the adaptation disease of freeze-thaw embryo transplantation, and excluding other factors possibly influencing embryo implantation, such as uterus abnormality, endocrine disorder and the like; s300, treatment before embryo transplantation; s310, formulating a personalized hCG administration scheme according to the specific condition of the patient, and suggesting to start intramuscular injection of hCG several days before embryo transplantation; s320, the dosage is adjusted according to factors such as the body weight, the age and the hormone level of the patient, and the dosage range is usually 2500-5000 IU. By adjusting the injection time and dosage of the hCG, the success rate of embryo implantation is remarkably increased, and the method has important significance on clinical treatment.

Description

Technical Field

[0001] The invention relates to the technical field of embryo transplantation, and in particular to a method for improving the implantation rate of frozen-thawed embryo transplantation in RIF patients. Background Art

[0002] Recurrent implantation failure (RIF) is defined as the failure to achieve clinical pregnancy after at least three high-quality embryos are transferred in three fresh or frozen cycles in women under 40 years of age. High-quality embryos include day 3 embryos (cell number ≥8, uniform blastomere size, fragmentation rate <10%) and blastocysts (≥3BB). This problem affects about 10% of in vitro fertilization-embryo transfer (IVF-ET) patients, bringing significant economic and psychological burdens. The etiology of RIF is complex, involving maternal factors (such as immune abnormalities, prothrombotic state, poor endometrial receptivity, infection, reproductive system anatomical abnormalities and endocrine disorders), male factors and embryonic factors. The embryo implantation process is complex and requires effective interaction between the embryo and the endometrium. Studies have shown that human chorionic gonadotropin (hCG) plays a key role in this process. Summary of the invention

[0003] In view of the above-mentioned shortcomings of the prior art, the present invention provides a method for improving the implantation rate of frozen-thawed embryo transplantation in RIF patients, which can effectively solve the problem of low embryo implantation success rate in the prior art.

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0005] The present invention provides a method for improving the implantation rate of frozen-thawed embryo transplantation in RIF patients, comprising the following steps:

[0006] S100: Select patients diagnosed with RIF for a comprehensive reproductive health assessment, including testing of endometrial thickness, hormone levels, and other indicators;

[0007] S200: Ensure that the patient meets the indications for frozen-thawed embryo transfer and exclude other factors that may affect embryo implantation, such as uterine abnormalities, endocrine disorders, etc.;

[0008] S300: embryo transfer pretreatment;

[0009] S310: Develop a personalized hCG dosing regimen based on the patient's specific situation. It is recommended to start intramuscular hCG injection a few days before embryo transfer.

[0010] S320: The dosage is adjusted according to factors such as the patient's weight, age, and hormone levels. The usual dosage range is 2500-5000 IU.

[0011] Further, the hCG administration regimen includes hCG administration method and hCG administration monitoring and adjustment;

[0012] The hCG administration method is intramuscular injection, and the injection site is selected in a muscle-rich area;

[0013] Injections were given daily for 7 days and ended before embryo transfer.

[0014] Furthermore, the hCG administration monitoring and adjustment includes:

[0015] During hCG administration, monitor the patient's hormone level changes and endometrial condition, track the effects through ultrasound examination and blood tests, and adjust the hCG administration dose and frequency in time according to the monitoring results to ensure the best treatment effect.

[0016] Furthermore, after hCG administration is completed, frozen-thawed embryo transfer is performed according to the conventional process. Ensure that the timing of embryo transfer matches the hCG administration regimen;

[0017] After transplantation, the patient's hormone level and embryo implantation status continue to be monitored, and auxiliary treatment measures such as luteal support are given.

[0018] Compared with the known prior art, the technical solution provided by the present invention has the following beneficial effects:

[0019] The invention significantly improves the success rate of embryo implantation by adjusting the injection time and dosage of hCG, which is of great significance to clinical treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 The figure is a flow chart of the method of the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] The present invention will be further described below in conjunction with the embodiments.

[0024] Example: Reference Figure 1 , a method for improving the implantation rate of frozen-thawed embryo transplantation in RIF patients, comprising the following steps:

[0025] S100: Select patients diagnosed with RIF for a comprehensive reproductive health assessment, including testing of endometrial thickness, hormone levels, and other indicators;

[0026] S200: Ensure that the patient meets the indications for frozen-thawed embryo transfer and exclude other factors that may affect embryo implantation, such as uterine abnormalities, endocrine disorders, etc.;

[0027] S300: embryo transfer pretreatment;

[0028] S310: Develop a personalized hCG dosing regimen based on the patient's specific situation. It is recommended to start intramuscular hCG injection a few days before embryo transfer.

[0029] S320: The dosage is adjusted according to factors such as the patient's weight, age, and hormone levels. The usual dosage range is 2500-5000 IU.

[0030] The hCG administration regimen includes hCG administration method and hCG administration monitoring and adjustment; the hCG administration method adopts intramuscular injection, and the injection site is selected in the muscle-rich area; the injection is performed daily for 7 days, and the injection is completed before embryo transplantation. The hCG administration monitoring and adjustment includes: during the hCG administration period, monitoring the patient's hormone level changes and endometrial conditions, tracking the effects through ultrasound examination and blood tests, and adjusting the hCG administration dose and frequency in time according to the monitoring results to ensure the best treatment effect; after the hCG administration is completed, frozen-thaw embryo transplantation is performed according to the conventional process. Ensure that the timing of embryo transplantation matches the hCG administration regimen; continue to monitor the patient's hormone level and embryo implantation after transplantation, and provide auxiliary treatment measures such as corpus luteum support.

[0031] In the clinical trial, 50 RIF patients were selected and randomly divided into an hCG injection group and a control group. The hCG injection group began to inject 5000IU hCG daily 7 days before embryo transfer. The control group did not receive hCG injection. Two weeks after embryo transfer, the embryo implantation rate was evaluated by ultrasound examination and blood test. The results showed that the embryo implantation rate in the hCG injection group was 25% higher than that in the control group.

[0032] Example 2: In another study, 30 RIF patients were selected to receive the same hCG injection regimen and compared with 30 RIF patients receiving conventional treatment. The results showed that the clinical pregnancy rate in the hCG injection group was 40%, while that in the control group was 25%.

[0033] Patient Screening and Preparation:

[0034] RIF patients were selected, i.e. women under 40 years old who had not achieved clinical pregnancy after transplanting more than 3 high-quality embryos in at least 3 cycles.

[0035] Conduct a comprehensive reproductive health assessment, including endometrial thickness, hormone levels, etc., to rule out other factors that may affect embryo implantation.

[0036] Embryo transfer pre-treatment:

[0037] Start intramuscular injection of 2000-5000 IU hCG one day before FET, once every other day for a total of 3 times.

[0038] A personalized dosing regimen is developed based on factors such as the patient's weight, age, and hormone levels.

[0039] hCG administration method:

[0040] Intramuscular injection is used, and muscle-rich areas such as the gluteus maximus or deltoid muscle of the upper arm are selected to ensure rapid absorption of the drug and reduce discomfort.

[0041] Monitoring and Adjustment:

[0042] During hCG administration, the patient's hormone levels and endometrial condition are closely monitored. Dosage and frequency are adjusted through ultrasound examinations and blood tests.

[0043] Embryo transfer and subsequent treatment:

[0044] Perform embryo transfer according to the routine procedure, ensuring that the hCG injection regimen matches the timing of the transfer.

[0045] After transplantation, hormone levels and embryo implantation status continue to be monitored, and auxiliary treatments such as luteal support are provided as needed.

[0046] Traditionally, hCG is used for ovulation induction and corpus luteum support. This program innovatively uses hCG before FET to improve endometrial receptivity and increase embryo implantation rate; through personalized administration, hCG administration is formulated according to the patient's specific physiological conditions to maximize the effect, and preventive intramuscular injection is used. hCG injection starts a few days before transplantation to improve the embryo implantation environment by regulating endometrial receptivity and maternal-fetal immune tolerance.

[0047] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for improving the implantation rate of frozen-thawed embryo transplantation in RIF patients, characterized in that: The following steps are involved: S100: Select patients diagnosed with RIF for a comprehensive reproductive health assessment, including testing of endometrial thickness, hormone levels, and other indicators; S200: Ensure that the patient meets the indications for frozen-thawed embryo transfer and exclude other factors that may affect embryo implantation, such as uterine abnormalities, endocrine disorders, etc.; S300: embryo transfer pretreatment; S310: Develop a personalized hCG dosing regimen based on the patient's specific situation. It is recommended to start intramuscular hCG injection a few days before embryo transfer. S320: The dosage is adjusted according to factors such as the patient's weight, age, and hormone levels. The usual dosage range is 2500-5000 IU.

2. A method for improving the implantation rate of frozen-thawed embryos in RIF patients according to claim 1, characterized in that: The hCG administration regimen includes hCG administration method and hCG administration monitoring and adjustment; The hCG administration method is intramuscular injection, and the injection site is selected in a muscle-rich area; Injections were given daily for 7 days and ended before embryo transfer.

3. A method for improving the implantation rate of frozen-thawed embryos in RIF patients according to claim 2, characterized in that: The hCG administration monitoring and adjustment includes: During hCG administration, monitor the patient's hormone level changes and endometrial condition, track the effects through ultrasound examination and blood tests, and adjust the hCG administration dose and frequency in time according to the monitoring results to ensure the best treatment effect.

4. A method for improving the implantation rate of frozen-thawed embryos in RIF patients according to claim 1, characterized in that: After hCG administration is completed, frozen-thawed embryo transfer is performed according to the conventional process. Ensure that the timing of embryo transfer matches the hCG administration regimen; After transplantation, the patient's hormone level and embryo implantation status continue to be monitored, and auxiliary treatment measures such as luteal support are given.

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