Composition with mild whitening and freckle removing effects and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly discloses a composition with mild whitening and freckle removing effects and application thereof, and the composition comprises a plant extract and a gastrodia elata extract. According to the composition, the plant extract and the gastrodia elata extract are added, the whitening effect is remarkably improved, and meanwhile, the composition with the mild whitening and freckle removing effects is a medicinal and edible composition and can increase and supplement nutrition, enhance immunity, nourish blood, tonify deficiency and regulate intestines and stomach.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine compositions, and in particular to a composition with mild whitening and freckle-removing effects and application thereof. Background Art

[0002] Long-term exposure to radiation from electronic screens such as mobile phones and computers can easily cause modern women's skin to become bumpy and dull. As a result, adverse symptoms such as dark skin, pigmentation, and spots are becoming more and more common. Improving skin color has become an area that modern humans pay more and more attention to, and the research and development of skin care products with whitening effects has also received much attention.

[0003] At present, the use and research of various whitening and freckle removal agents have promoted the development of the whitening and freckle removal industry, but also aroused people's concern about the safety of whitening and freckle removal agents. There are reports that the combined use or excessive use of whitening ingredients may cause adverse skin reactions. For example, long-term use of kojic acid may cause contact dermatitis and allergic reactions.

[0004] Therefore, providing a mild whitening and freckle-removing product has become a technical problem that needs to be solved urgently by those skilled in the art. Summary of the invention

[0005] The present invention provides a composition with mild whitening and freckle-removing effects and application thereof, which has excellent whitening effect. Meanwhile, the composition with mild whitening and freckle-removing effects is a medicine and food homologous composition, which can increase nutrition, enhance immunity, nourish blood and replenish deficiency, and regulate the stomach.

[0006] The present invention solves the technical problem by adopting the following technical solutions: A composition with mild whitening and freckle removal effects, comprising a plant extract and a gastrodia elata extract, wherein the mass ratio of the plant extract to the gastrodia elata extract is 1:(0.2-0.6); The preparation method of the plant extract comprises the following steps: Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder; Add Cistanche deserticola powder, Cornus officinalis powder, Eucommia ulmoides leaf powder and glycerin into water, stir evenly, and boil to obtain a decoction; The decoction is placed on a macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV is washed with deionized water and 2 BV is washed with 80 wt% ethanol solution. The ethanol washings are collected to obtain the plant extracts.

[0007] As a preferred embodiment of the present invention, the mass ratio of the Cistanche deserticola powder, Cornus officinalis powder and Eucommia ulmoides leaf powder is 1:(0.5-2):(1-4).

[0008] As a preferred embodiment of the present invention, the mass ratio of the Cistanche deserticola powder, glycerin and water is 1: (6-10): (15-30).

[0009] As a preferred embodiment of the present invention, the macroporous adsorption resin is D101.

[0010] As a preferred embodiment of the present invention, the method for preparing the Gastrodia elata extract comprises the following steps: Gastrodia elata is crushed to obtain gastrodia elata powder; Adding gastrodia elata powder and complex enzyme into water, performing enzymolysis, filtering and drying, and obtaining gastrodia elata extract.

[0011] As a preferred embodiment of the present invention, the mass ratio of Gastrodia elata, complex enzyme and water is 1: (0.01-0.06): (2-10).

[0012] As a preferred embodiment of the present invention, the complex enzyme comprises pectinase, flavor protease and trypsin; The mass ratio of the pectinase, flavor protease and trypsin is 1:(0.5-2):(0.2-1).

[0013] The present invention also provides use of a composition with mild whitening and freckle-removing effects in preparing whitening and freckle-removing products.

[0014] The beneficial effects of the present invention are as follows: (1) The composition of the present invention significantly improves the whitening effect by adding plant extracts and gastrodia extracts. The plant extracts are plant-derived, mild to the skin, and will not cause allergic reactions. The plant extracts use Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves as substrates. Cistanche deserticola can inhibit the activity of tyrosinase and the production of melanin in human epidermal melanocytes; Cornus officinalis helps to inhibit the activity of tyrosinase and reduce melanin synthesis, thereby preventing and fading spots and brightening the overall skin color. At the same time, Cornus officinalis can promote epidermal cell metabolism. metabolism, so that the old stratum corneum containing pigments can be normally shed and new cells can be quickly replenished, thereby fundamentally improving uneven skin color; Eucommia leaves have excellent antioxidant effects, and can also reduce tyrosinase activity and UVB-induced melanin content in A375 cells; the three whiten from different whitening mechanisms, and then play a synergistic effect, and jointly improve the whitening effect of plant extracts; at the same time, the mild whitening and freckle-removing composition is a medicine and food composition, which can increase nutrition, enhance immunity, nourish blood and replenish deficiency, and regulate the stomach. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0017] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0018] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0019] The reagents or instruments used in the present invention without indicating the manufacturer are all conventional products that can be obtained through commercial purchase.

[0020] Among them, the Cistanche deserticola, Cornus officinalis, Eucommia ulmoides leaves and Gastrodia elata of the present invention were purchased from Guangzhou Qingping Chinese medicinal materials market.

[0021] The embodiment of the present invention provides a composition with mild whitening and freckle removal effects, comprising a plant extract and a gastrodia extract, wherein the mass ratio of the plant extract to the gastrodia extract is 1:(0.2-0.6); The preparation method of the plant extract comprises the following steps: Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder; Add Cistanche deserticola powder, Cornus officinalis powder, Eucommia ulmoides leaf powder and glycerin into water, stir evenly, and boil to obtain a decoction; The decoction is placed on a macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV is washed with deionized water and 2 BV is washed with 80 wt% ethanol solution. The ethanol washings are collected to obtain the plant extracts.

[0022] The invention obtains a plant extract with excellent whitening effect by mixed extraction of Cistanche deserticola, Cornus officinalis and Eucommia ulmoides leaves.

[0023] Among them, Cistanche, Cornus officinalis and Eucommia leaf are all indispensable. When used together, as a substrate, Cistanche can inhibit the activity of tyrosinase and the production of melanin in human epidermal melanocytes; Cornus officinalis helps to inhibit the activity of tyrosinase and reduce melanin synthesis, thereby preventing and fading spots and brightening the overall skin tone. At the same time, Cornus officinalis can promote the metabolism of epidermal cells, so that the old stratum corneum containing pigments can be shed normally and new cells can be quickly replenished, thereby fundamentally improving uneven skin tone; Eucommia leaf has excellent antioxidant effects and can also reduce the tyrosinase activity and UVB-induced melanin content in A375 cells; the three whiten from different whitening mechanisms, thereby exerting a synergistic effect and jointly improving the whitening effect of plant extracts.

[0024] In one embodiment, the mass ratio of Cistanche deserticola powder, Cornus officinalis powder and Eucommia ulmoides leaf powder is 1:(0.5-2):(1-4).

[0025] In one embodiment, the mass ratio of Cistanche deserticola powder, glycerin and water is 1: (6-10): (15-30).

[0026] In one embodiment, the macroporous adsorption resin is D101.

[0027] In one embodiment, the method for preparing the Gastrodia elata extract comprises the following steps: Gastrodia elata is crushed to obtain gastrodia elata powder; Adding gastrodia elata powder and complex enzyme into water, performing enzymolysis, filtering and drying, and obtaining gastrodia elata extract.

[0028] In one embodiment, the mass ratio of Gastrodia elata, complex enzyme and water is 1: (0.01-0.06): (2-10).

[0029] In one embodiment, the complex enzyme includes pectinase, flavor protease and trypsin; The mass ratio of the pectinase, flavor protease and trypsin is 1:(0.5-2):(0.2-1).

[0030] One embodiment of the present invention provides a use of a composition with mild whitening and freckle-removing effects in the preparation of a whitening and freckle-removing product.

[0031] The present invention is further described below with reference to specific embodiments.

[0032] Example 1 A composition with mild whitening and freckle-removing effects comprises a plant extract and a gastrodia elata extract, wherein the mass ratio of the plant extract to the gastrodia elata extract is 1:0.5.

[0033] The preparation method of the plant extract comprises the following steps: (1) Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves into 100 meshes to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder, respectively; (2) Add 1 part by weight of Cistanche deserticola powder, 1 part by weight of Cornus officinalis powder, 2 parts by weight of Eucommia ulmoides leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stir at 600 rpm for 2 h, and boil for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0034] The preparation method of the Gastrodia elata extract comprises the following steps: (1) Grinding the Gastrodia elata into 200 meshes to obtain Gastrodia elata powder; (2) Add Gastrodia elata powder and complex enzyme into water, perform enzymolysis at 50°C for 5 h, filter, and dry to obtain Gastrodia elata extract.

[0035] The mass ratio of the gastrodia elata, the complex enzyme and water is 1:0.05:5.

[0036] The complex enzyme comprises pectinase, flavor protease and trypsin; the mass ratio of the pectinase, flavor protease and trypsin is 1:1:0.5.

[0037] Example 2 A composition with mild whitening and freckle-removing effects comprises a plant extract and a gastrodia elata extract, wherein the mass ratio of the plant extract to the gastrodia elata extract is 1:0.2.

[0038] The preparation method of the plant extract comprises the following steps: (1) Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves into 100 meshes to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder, respectively; (2) Add 1 part by weight of Cistanche deserticola powder, 2 parts by weight of Cornus officinalis powder, 1 part by weight of Eucommia ulmoides leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stir at 600 rpm for 2 h, and boil for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0039] The preparation method of the Gastrodia elata extract comprises the following steps: (1) Grinding the Gastrodia elata into 200 meshes to obtain Gastrodia elata powder; (2) Add Gastrodia elata powder and complex enzyme into water, perform enzymolysis at 50°C for 5 h, filter, and dry to obtain Gastrodia elata extract.

[0040] The mass ratio of the gastrodia elata, the complex enzyme and water is 1:0.05:5.

[0041] The complex enzyme comprises pectinase, flavor protease and trypsin; the mass ratio of the pectinase, flavor protease and trypsin is 1:1:0.5.

[0042] Example 3 A composition with mild whitening and freckle-removing effects comprises a plant extract and a gastrodia elata extract, wherein the mass ratio of the plant extract to the gastrodia elata extract is 1:0.6.

[0043] The preparation method of the plant extract comprises the following steps: (1) Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves into 100 meshes to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder, respectively; (2) Add 1 part by weight of Cistanche deserticola powder, 1 part by weight of Cornus officinalis powder, 3 parts by weight of Eucommia ulmoides leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stir at 600 rpm for 2 h, and boil for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0044] The preparation method of the Gastrodia elata extract comprises the following steps: (1) Grinding the Gastrodia elata into 200 meshes to obtain Gastrodia elata powder; (2) Add Gastrodia elata powder and complex enzyme into water, perform enzymolysis at 50°C for 5 h, filter, and dry to obtain Gastrodia elata extract.

[0045] The mass ratio of the gastrodia elata, the complex enzyme and water is 1:0.05:5.

[0046] The complex enzyme comprises pectinase, flavor protease and trypsin; the mass ratio of the pectinase, flavor protease and trypsin is 1:1:0.5.

[0047] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain plant extracts, and the other parts are the same, that is, Comparative Example 1 is a single Gastrodia elata extract.

[0048] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that in the preparation method of the plant extract in Comparative Example 2, no Cistanche powder is added (the total amount of Cistanche powder, Cornus officinalis powder, and Eucommia leaf powder remains unchanged), and the rest are the same.

[0049] The preparation method of the plant extract comprises the following steps: (1) Grinding cornus officinalis and eucommia ulmoides leaves into 100 meshes respectively to obtain cornus officinalis powder and eucommia ulmoides leaf powder; (2) Add 1.5 parts by weight of Cornus officinalis powder, 2.5 parts by weight of Eucommia ulmoides leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stir at 600 rpm for 2 h, and boil for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0050] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that in the preparation method of the plant extract in Comparative Example 3, no Cornus officinalis powder is added (the total amount of Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder remains unchanged), and the rest are the same.

[0051] The preparation method of the plant extract comprises the following steps: (1) Crush the Cistanche deserticola and Eucommia ulmoides leaves into 100 meshes respectively to obtain Cistanche deserticola powder and Eucommia ulmoides leaf powder respectively; (2) Add 1.5 parts by weight of Cistanche deserticola powder, 2.5 parts by weight of Eucommia leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stir at 600 rpm for 2 h, and boil for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0052] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that in the preparation method of the plant extract in Comparative Example 4, no eucommia leaf powder is added (the total amount of Cistanche powder, Cornus officinalis powder, and eucommia leaf powder remains unchanged), and the rest are the same.

[0053] The preparation method of the plant extract comprises the following steps: (1) Crush Cistanche deserticola and Cornus officinalis to 100 meshes to obtain Cistanche deserticola powder and Cornus officinalis powder respectively; (2) adding 2 parts by weight of Cistanche deserticola powder, 2 parts by weight of Cornus officinalis powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 h, and boiling for 10 h to obtain a decoction; (3) The decoction was applied to D101 macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV was washed with deionized water and 2 BV was washed with 80 wt% ethanol solution. The ethanol washing solution was collected, which was the plant extract.

[0054] Comparative Example 5 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 is not treated with a macroporous adsorption resin, and the other aspects are the same.

[0055] The preparation method of the plant extract comprises the following steps: (1) Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves into 100 meshes to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder, respectively; (2) adding 1 part by weight of Cistanche deserticola powder, 1 part by weight of Cornus officinalis powder, 2 parts by weight of Eucommia ulmoides leaf powder, and 6 parts by weight of glycerin to 20 parts by weight of water, stirring at 600 rpm for 2 hours, and boiling for 10 hours to obtain a decoction to obtain a plant extract;

[0056] Comparative Example 6 The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain Gastrodia elata extract, and everything else is the same, that is, Comparative Document 1 is a single plant extract.

[0057] Test Case The compositions of the examples and comparative examples were prepared into 0.2 mg / mL test solutions with phosphate buffer solution at pH 6.8, respectively. 30 μL of the test solution was taken and prepared into 1 mL with 970 μL of phosphate buffer solution at pH 6.8, 1 mL of 0.1 mg / mL tyrosine was added, and then 1 mL of tyrosinase (200 U / mg) prepared with phosphate buffer solution at pH 6.8 was added, incubated at 37°C for 20 min, and the absorbance was measured at 475 nm.

[0058] Each experiment was repeated three times, and the average value was taken as the final result. The tyrosinase activity inhibition rate was determined according to the following formula; the average value of the test results is shown in Table 1.

[0059] Tyrosinase activity inhibition rate ; : absorbance in the absence of tyrosinase inhibitor; : absorbance when only tyrosine is present; : absorbance of tyrosinase and tyrosine in the presence of inhibitors; : Absorbance in the absence of tyrosinase.

[0060] Table 1

[0061] It can be seen from Table 1 that the composition of the present invention has an excellent whitening effect.

[0062] Comparing Examples 1 to 3, it can be seen that Example 1 is the best embodiment of the present invention and has the best whitening effect.

[0063] By comparing Example 1 with Comparative Example 1, it can be seen that the plant extract of the present invention has a very significant improvement in the whitening effect.

[0064] By comparing Example 1 with Comparative Examples 2 to 4, it can be seen that the Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves described in the present invention are all indispensable in the preparation of the plant extract. The combination of the three has an excellent synergistic effect. If any one of them is missing, the whitening effect will be significantly reduced.

[0065] By comparing Example 1 with Comparative Example 5, it can be seen that the present invention further improves the whitening effect by performing macroporous adsorption resin treatment.

[0066] By comparing Example 1 with Comparative Examples 1 and 6, it can be seen that, under the same test concentration, the whitening effect of a single Gastrodia elata extract or a single plant extract of the present invention is significantly worse than that of the composition of the present invention, which shows that the Gastrodia elata extract and the plant extract of the present invention have a certain synergistic effect.

[0067] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition with mild whitening and freckle removing effect, characterized in that: It includes a plant extract and a gastrodia extract, wherein the mass ratio of the plant extract to the gastrodia extract is 1:(0.2-0.6); The preparation method of the plant extract comprises the following steps: Crush Cistanche deserticola, Cornus officinalis, and Eucommia ulmoides leaves to obtain Cistanche deserticola powder, Cornus officinalis powder, and Eucommia ulmoides leaf powder; Add Cistanche deserticola powder, Cornus officinalis powder, Eucommia ulmoides leaf powder and glycerin into water, stir evenly, and boil to obtain a decoction; The decoction is placed on a macroporous adsorption resin and eluted at a rate of 1.5 BV / h. 1.5 BV is washed with deionized water and 2 BV is washed with 80 wt% ethanol solution. The ethanol washings are collected to obtain the plant extracts.

2. The composition with mild whitening and freckle-removing effect according to claim 1, characterized in that: The mass ratio of the Cistanche deserticola powder, Cornus officinalis powder and Eucommia ulmoides leaf powder is 1:(0.5-2):(1-4).

3. The composition with mild whitening and freckle removing effect according to claim 1, characterized in that: The mass ratio of the Cistanche deserticola powder, glycerin and water is 1: (6-10): (15-30).

4. The composition with mild whitening and freckle removing effect according to claim 1, characterized in that: The macroporous adsorption resin is D101.

5. The composition with mild whitening and freckle removing effect according to claim 1, characterized in that: The preparation method of the Gastrodia elata extract comprises the following steps: Gastrodia elata is crushed to obtain gastrodia elata powder; Adding gastrodia elata powder and complex enzyme into water, performing enzymolysis, filtering and drying, and obtaining gastrodia elata extract.

6. The composition with mild whitening and freckle-removing effect according to claim 5, characterized in that: The mass ratio of the gastrodia elata, the complex enzyme and water is 1: (0.01-0.06): (2-10).

7. The composition with mild whitening and freckle removing effect according to claim 5, characterized in that: The complex enzyme comprises pectinase, flavor protease and trypsin.

8. The composition with mild whitening and freckle-removing effect according to claim 5, characterized in that: The mass ratio of the pectinase, flavor protease and trypsin is 1:(0.5-2):(0.2-1).

9. Use of the composition with mild whitening and freckle-removing effect according to any one of claims 1 to 9 in the preparation of whitening and freckle-removing products.

Images