Essence oil containing ceramide and preparation method thereof

Abstract

The invention provides essential oil containing ceramide and a preparation method of the essential oil. The essential oil containing ceramide is prepared from ceramide, mannosyl erythritol lipid, synthetic grease and natural vegetable grease. According to the ceramide-containing essential oil disclosed by the invention, complex components such as a surfactant and a stabilizer do not need to be added, and the added mannosyl erythritol lipid is natural sugar ester and has good biocompatibility and safety, so that the ceramide-containing essential oil disclosed by the invention is mild and non-irritant to skin and has excellent product safety; besides, according to the pure oil formulation formula, the purpose of improving the solubility and stability of ceramide can be achieved only by adding mannosyl erythritol lipid, the skin barrier maintaining effect of ceramide can be better played, the anti-inflammatory and skin repairing effects of ceramide are improved, and the skin health level is improved; the application range of ceramide in cosmetics is expanded.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a ceramide-containing essence oil and a preparation method thereof. Background Art

[0002] Skin is the first barrier on the human body surface. It covers the whole body and can protect various parts of the human body from physical and chemical damage caused by harmful external factors. It also prevents the loss of nutrients, water and electrolytes in the body. It plays an important role in maintaining the stability of the human body's internal environment. Due to the constant changes in the environment and the increase in age, human skin is inevitably damaged. In daily care, the barrier function of the skin can be strengthened by using appropriate skin care products.

[0003] Ceramide is an important lipid molecule that exists in the cell membrane of organisms, especially in the skin. Ceramide is essential for maintaining the barrier function of the skin, helping to lock in moisture and prevent skin dryness and damage. In addition, ceramide also has anti-inflammatory and skin repair effects. Due to its important physiological function, ceramide has broad application prospects in the fields of cosmetics, medicine and food.

[0004] In the field of cosmetics, in order to improve the solubility and utilization rate of ceramide, existing technologies mainly use methods such as liquid crystal emulsification and liposome encapsulation. These methods encapsulate ceramide in lamellar liquid crystals or liposomes, such as the common nano-encapsulated ceramide, ceramide liquid crystal cream, ceramide liposomes, etc. on the market, thereby improving its solubility and stability, thereby increasing the use effect of ceramide in cosmetics.

[0005] However, it cannot be ignored that these existing methods for promoting the solubility and stability of ceramides have some problems and limitations. First, the preparation process of these methods is relatively complicated and often requires the use of special equipment and processes, such as high-pressure homogenizers and microfluidics, which will increase production costs; second, these methods usually require the addition of other dissolution-aiding ingredients, such as surfactants and stabilizers, which may irritate the skin and affect the safety of the product; in addition, these methods are usually not suitable for pure oil dosage form formulations, and cannot form a stable and transparent state in oils and fats, which limits their application in cosmetic dosage forms such as essence oils.

[0006] In view of this, developing a simple, safe method for improving the solubility and stability of ceramides that is suitable for pure oil dosage forms is an important technical challenge currently faced. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention discloses a ceramide-containing essential oil and a preparation method thereof. The ceramide-containing essential oil does not require the addition of other solubilizing ingredients, is mild and non-irritating, has sufficient ceramide solubility and high stability, maintains a translucent state, helps maintain the skin barrier function and exerts anti-inflammatory and skin repair effects; the preparation method of the ceramide-containing essential oil is simple to operate and effectively reduces the production cost.

[0008] In order to achieve the above technical objectives, on the one hand, the present invention provides a ceramide-containing essential oil, wherein the ceramide-containing essential oil is composed of ceramide, mannosylerythritol lipid, synthetic oil and natural plant oil.

[0009] The research and development team of the present invention unexpectedly discovered through exploratory experiments that although adding commonly used low HLB emulsifiers such as cetyl PEG / PPG-10 / 1 polydimethylsiloxane, lauryl PEG-9 polydimethylsiloxyethyl polydimethylsiloxane, sorbitan sesquiisostearate, etc. to the pure oil formulation containing ceramide can promote the dissolution of ceramide, the stability of the obtained essential oil is relatively poor; however, when mannosyl erythritol lipids are added to the formula containing synthetic oils and natural plant oils, not only the solubility of ceramide can be improved, but also the stability of the obtained essential oil can be effectively improved, and the translucent state can still be maintained at room temperature and low temperature for 1 month, thus the above technical scheme is proposed.

[0010] In a further example of the present invention, the dosage of the components in the ceramide-containing essential oil is explored and optimized. Optionally, the ceramide-containing essential oil includes: 0.01-0.15 parts of ceramide; 0.05-2 parts of mannosylerythritol lipids; 1-25 parts of synthetic oils; and the balance is natural plant oils.

[0011] In a further example of the present invention, the dosage of the components in the ceramide-containing essential oil and the optional types of each component are further optimized, and the above optimization process is shown in the embodiments of the present invention.

[0012] On the other hand, the present invention provides a method for preparing the above-mentioned ceramide-containing essential oil, which comprises: (1) dissolving ceramide and mannosylerythritol in synthetic oil in sequence at 84-95° C. to obtain a first material; (2) cooling the first material to no higher than 50° C., adding natural plant oil and mixing evenly to obtain the ceramide-containing essential oil.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The ceramide-containing essence oil of the present invention uses only mannosylerythritol lipid, and does not need to add complex ingredients such as surfactants and stabilizers. The added mannosylerythritol lipid is a natural sugar ester with good biocompatibility and safety. Therefore, the ceramide-containing essence oil of the present invention is mild and non-irritating to the skin, and the product safety is excellent. In addition, the pure oil dosage form formula of the present invention can achieve the purpose of improving the solubility and stability of ceramide by adding mannosylerythritol lipid only, which can better play the role of ceramide in maintaining the skin barrier, and enhance its anti-inflammatory and skin repairing effects, thereby improving the skin health level; and expanding the application scope of ceramide in cosmetics.

[0015] Compared with the more complicated liquid crystal emulsification and liposome encapsulation of ceramide processes in the existing processes, the preparation method of the ceramide-containing essential oil of the present invention is very simple to operate, does not require the use of special process operations and special equipment, greatly reduces the production cost, and has important promotion and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The drawings constituting a part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0017] Figure 1 A comparative diagram showing the stability test of the essential oil at room temperature shown in Example 1 of the present invention and Comparative Example 1 is shown;

[0018] Figure 2 The moisturizing performance test results of the ceramide-containing essence oil of the present invention are shown;

[0019] Figure 3 The cytotoxicity test results of the ceramide-containing essential oil of the present invention are shown;

[0020] Figure 4 The anti-inflammatory property test results of the ceramide-containing essential oil of the present invention are shown;

[0021] Figure 5 The TEWL test results of the ceramide-containing essence oil of the present invention are shown. DETAILED DESCRIPTION

[0022] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively below, and preferred embodiments of the present invention are given. However, it should be understood that these embodiments are only used for more detailed description and should not be understood as limiting the present invention in any form, i.e., not intended to limit the scope of protection of the present invention.

[0023] Unless otherwise defined, the technical terms used in the following examples have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods, unless otherwise specified, are all conventional methods.

[0024] One aspect of the present invention provides a ceramide-containing essential oil, wherein the ceramide-containing essential oil is composed of ceramide, mannosylerythritol lipid, synthetic oil and natural plant oil.

[0025] In a further example of the present invention, the ceramide-containing essential oil comprises: 0.01-0.15 parts of ceramide; 0.05-2 parts of mannosylerythritol lipids; 1-25 parts of synthetic oils; and the remainder is natural plant oils.

[0026] In the technical solution of the present invention, when the ceramide is 0.01 parts, it can produce better moisturizing, anti-inflammatory and skin repairing effects. When its weight fraction is too high, it may affect the stability of the overall essence oil. Therefore, the weight fraction of ceramide optimized by the present invention is 0.01-0.15 parts, and can be 0.01 parts, 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, 0.11 parts, 0.12 parts, 0.13 parts, 0.14 parts, and 0.15 parts, but not limited to the listed range, other values ​​not listed within the above numerical range are also applicable to the present invention.

[0027] In addition, the researchers of the present invention have found that adding an appropriate amount of mannosylerythritol lipid to the ceramide-containing essential oil of the present invention can effectively promote the dissolution of ceramide and make the essential oil show excellent stability at room temperature and low temperature conditions. Therefore, the weight portion of mannosylerythritol lipid optimized by the present invention is 0.05-2 parts, and 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, 0.11 parts, 0.12 parts, 0.13 parts, 0.14 parts, 0.15 parts, 0.16 parts, 0.17 parts, 0.18 parts, 0.19 parts, and 0.2 parts can be selected, but are not limited to the listed ranges, and other values ​​not listed within the above numerical ranges are also applicable to the present invention.

[0028] In addition, the present invention is a pure oil formulation comprising synthetic oils and natural plant oils, which can give full play to the stability of synthetic oils and the nourishing, repairing and mild skin feel characteristics of natural plant oils. The dosage of synthetic oils and natural oils is optimized in the present invention, wherein the weight portion of synthetic oils is 1-25 parts, which can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, but is not limited to the listed range, and other values ​​not listed within the above numerical range are also applicable to the present invention, and the balance in the essence oil is natural plant oil, and the synergistic cooperation of the two oils enhances the function of ceramide in promoting the maintenance of the barrier and repairing the skin.

[0029] Based on a large amount of experimental data, in a further example of the present invention, the components of the ceramide-containing essential oil are optimized. Optionally, in parts by weight, the ceramide-containing essential oil includes 0.01-0.1 parts of ceramide, 0.1-1 parts of mannosylerythritol lipids, 3-18 parts of synthetic oils, and the remainder is natural plant oils.

[0030] In a further example of the present invention, the ceramide-containing essential oil includes 0.3-0.8 parts of mannosylerythritol lipid, and optionally 0.3-0.5 parts of mannosylerythritol lipid, by weight.

[0031] In a further example of the present invention, the types of ceramides are explored and optimized. Optionally, the ceramides include one or more of ceramide AP, ceramide NP, ceramide NS, and cetyl-PG hydroxyethyl palmitamide. By selecting the appropriate type of ceramide, the dissolution of the components can be promoted and the stability of the obtained essential oil can be improved. The effects of different types of ceramides on the stability of the obtained essential oil are shown in the embodiments and comparative examples of the present invention.

[0032] In a further example of the present invention, the types of synthetic oils are explored and optimized. Optionally, the synthetic oils include one or two of triglycerides and monoester oils. Further optionally, the triglycerides are selected from one or more of caprylic / capric triglycerides, caprylic / capric / succinic triglycerides, and tri(ethylhexanoic)glycerol. Further optionally, the monoester oils are selected from one or more of coconut oil alcohol-caprylate / caprate, ethylhexyl palmitate, isopropyl myristate, and isopropyl isostearate. Those skilled in the art can make selections as needed in actual preparation.

[0033] In a further example of the present invention, the type of the natural plant oil is explored and optimized. Optionally, the natural plant oil includes filbert (CORYLUS AVELLANA) seed oil, grape (VITIS VINIFERA) seed oil, wheat (TRITICUM VULGARE) germ oil, safflower (CARTHAMUS TINCTORIUS) seed oil, sweet almond (PRUNUSAMYGDALUSDULCIS) oil, rapeseed (BRASSICA CAMPESTRIS) seed oil, oat (AVENA SATIVA) kernel oil, avocado tree (BUTYROSPERMUM PARKII) fruit fat, spearmint (MENTHA VIRIDIS) leaf oil, cardamom (ELETTARIACARDAMOMUM) seed oil, white pond flower (LIMNANTHES ALBA) seed oil, canola oil, carrot (DAUCUS CAROTASATIVA) seed oil, sunflower (HELIANTHUS ANNUUS) seed oil, sea buckthorn (HIPPOPHAE One or more of RHAMNOIDES) seed oils are beneficial to dissolving ceramide and improving the stability of the essential oil. In the actual preparation process, technicians in this field can select them according to needs.

[0034] In a further example of the present invention, an essential oil optionally containing ceramides is shown:

[0035] In terms of weight, the ceramide-containing essential oil comprises: 0.1 parts of ceramide; 1 part of mannosylerythritol lipid; 17 parts of synthetic oil; and the balance is natural plant oil;

[0036] Alternatively, the ceramide-containing essential oil comprises: 0.03 parts of ceramide; 0.5 parts of mannosylerythritol lipid; 15 parts of synthetic oil; and the remainder is natural plant oil;

[0037] Alternatively, the ceramide-containing essential oil comprises: 0.01 parts of ceramide; 0.8 parts of mannosylerythritol lipid; 18 parts of synthetic oil; and the remainder is natural plant oil;

[0038] Alternatively, the ceramide-containing essential oil comprises: 0.05 parts of ceramide; 0.3 parts of mannosylerythritol lipid; 6 parts of synthetic oil; and the remainder is natural plant oil;

[0039] Alternatively, the ceramide-containing essential oil comprises: 0.1 parts of ceramide; 0.1 parts of mannosylerythritol lipids; 3 parts of synthetic oils; and the remainder is natural plant oils.

[0040] Another aspect of the present invention provides a method for preparing the above-mentioned ceramide-containing essential oil, which comprises: (1) dissolving ceramide and mannosylerythritol in synthetic oil in sequence at 84-95° C. to obtain a first material; (2) cooling the first material to no higher than 50° C., adding natural plant oil and mixing evenly to obtain the ceramide-containing essential oil.

[0041] It should be noted that the present invention is not limited to the equipment in the above preparation process, and any equipment that can provide contact and mixing of ceramide, mannosylerythritol lipids, synthetic oils and natural plant oils is sufficient. In addition, in order to promote the dissolution and mixing of the components, those skilled in the art may optionally perform stirring or other operations to promote the mixing and contact of the materials in step (1) and step (2), and the protection scope of the present invention is not limited thereby.

[0042] Furthermore, the present invention also includes cooling the natural plant oil and the first mixed material after they are evenly mixed, such as cooling to about 30-40° C., to obtain the ceramide-containing essential oil at a suitable temperature for easy storage and subsequent use.

[0043] Example 1

[0044] A ceramide-containing essential oil, which comprises, by weight, 0.1 parts of ceramide, including 0.03 parts of ceramide AP, 0.01 parts of ceramide NP, 0.01 parts of ceramide NS, and 0.05 parts of cetyl-PG hydroxyethyl palmitamide; 1 part of mannosyl erythritol lipid; 17 parts of synthetic oil, including 6 parts of caprylic capric triglyceride, 6 parts of coconut oil alcohol-caprylate caprate, and 5 parts of isopropyl isostearate; 81.9 parts of natural vegetable oil, including 30 parts of filbert oil, 21.9 parts of grape seed oil, 18 parts of wheat germ oil, 10 parts of oat kernel oil, and 2 parts of avocado tree butter.

[0045] The ceramide-containing essential oil of this embodiment is prepared by the following method: (1) adding mannosylerythritol lipid to a ceramide / synthetic oil mixed solution at about 85-95° C., stirring until dissolved to obtain a first material; (2) slowly cooling the first material to below 50° C. while stirring, then adding natural plant oil, stirring and mixing evenly; cooling to about 35° C. and discharging the material.

[0046] Example 2

[0047] A ceramide-containing essential oil, which comprises, by weight, 0.03 parts of ceramide, namely 0.03 parts of ceramide NP; 0.5 parts of mannosyl erythritol lipids; 15 parts of synthetic oils, including 4 parts of caprylic capric triglyceride, 5 parts of coconut oil alcohol-caprylate caprate, and 6 parts of isopropyl isostearate; and 84.47 parts of natural plant oils, including 38.47 parts of filbert oil, 25 parts of grape seed oil, 20 parts of oat kernel oil, and 1 part of avocado tree butter.

[0048] The preparation process of the ceramide-containing essential oil in this example is the same as that in Example 1.

[0049] Example 3

[0050] A ceramide-containing essential oil, which comprises, by weight, 0.01 parts of ceramide, namely 0.01 parts of ceramide NS; 0.8 parts of mannosyl erythritol lipids; 18 parts of synthetic oils, including 10 parts of coconut oil monocaprylate caprate and 8 parts of isopropyl isostearate; and 81.19 parts of natural vegetable oils, including 40 parts of filbert oil, 10 parts of grape seed oil, 1.19 parts of wheat germ oil and 30 parts of oat kernel oil.

[0051] The preparation process of the ceramide-containing essential oil in this example is the same as that in Example 1.

[0052] Example 4

[0053] A ceramide-containing essential oil, which comprises, by weight, 0.5 parts of ceramide, namely 0.05 parts of ceramide AP; 0.3 parts of mannosyl erythritol lipids; 6 parts of synthetic oils, including 3 parts of caprylic capric triglyceride, 1 part of coconut oil alcohol-caprylate caprate, and 2 parts of isopropyl isostearate; and 93.65 parts of natural plant oils, including 3.65 parts of filbert oil, 35 parts of grape seed oil, 30 parts of wheat germ oil, 15 parts of oat kernel oil, and 10 parts of avocado tree butter.

[0054] The preparation process of the ceramide-containing essential oil in this example is the same as that in Example 1.

[0055] Example 5

[0056] A ceramide-containing essential oil, which comprises, by weight, 0.1 parts of ceramide, which is 0.1 parts of cetyl-PG hydroxyethyl palmitamide; 0.1 parts of mannosyl erythritol lipids; 3 parts of synthetic oils, which is 3 parts of caprylic capric triglyceride; and 96.8 parts of natural plant oils, including 63.8 parts of filbert oil, 20 parts of wheat germ oil, 8 parts of oat kernel oil, and 5 parts of avocado tree fat.

[0057] The preparation process of the ceramide-containing essential oil in this example is the same as that in Example 1.

[0058] Comparative Example 1

[0059] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 1, except that mannosyl erythritol lipid is not added in this comparative example, and the weight portion of natural plant oil is 82.9 parts, including 31 parts of filbert oil, 21.9 parts of grape seed oil, 18 parts of wheat germ oil, 10 parts of oat kernel oil, and 2 parts of avocado tree fat.

[0060] Comparative Example 2

[0061] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 2, except that mannosyl erythritol lipid is not added in this comparative example, and the weight proportion of natural plant oil is 84.97 parts, including 38.97 parts of filbert oil, 25 parts of grape seed oil, 20 parts of oat kernel oil, and 1 part of avocado tree fat.

[0062] Comparative Example 3

[0063] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 3, except that mannosyl erythritol lipid is not added in this comparative example, and the weight portion of natural vegetable oil is 81.99 parts, including 40.8 parts of filbert oil, 10 parts of grape seed oil, 1.19 parts of wheat germ oil, and 30 parts of oat kernel oil.

[0064] Comparative Example 4

[0065] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 4, except that mannosyl erythritol lipid is not added in this comparative example, and the weight portion of natural vegetable oil is 93.95 parts, including 3.95 parts of filbert oil, 35 parts of grape seed oil, 30 parts of wheat germ oil, 15 parts of oat kernel oil, and 10 parts of avocado tree fat.

[0066] Comparative Example 5

[0067] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 5, except that mannosyl erythritol lipid is not added in this comparative example, and the weight portion of natural vegetable oil is 96.9 parts, including 63.9 parts of filbert oil, 20 parts of wheat germ oil, 8 parts of oat kernel oil, and 5 parts of avocado tree fat.

[0068] Comparative Example 6

[0069] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 1, except that the ceramide used in this comparative example includes 0.05 parts by weight of ceramide EOP and 0.05 parts by weight of ceramide AS.

[0070] Comparative Example 7

[0071] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 2, except that the ceramide used in this comparative example includes 0.03 parts by weight of ceramide EOP.

[0072] Comparative Example 8

[0073] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 3, except that the ceramide used in this comparative example includes 0.01 parts by weight of ceramide AS.

[0074] Comparative Example 9

[0075] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 1, except that mannosyl erythritol lipid is not added in this comparative example, but 1 part by weight of cetyl PEG / PPG-10 / 1 polydimethylsiloxane is added.

[0076] Comparative Example 10

[0077] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 2, except that mannosyl erythritol lipid is not added in this comparative example, but 0.5 parts by weight of lauryl PEG-9 polydimethylsiloxyethyl polydimethicone is added.

[0078] Comparative Example 11

[0079] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 3, except that mannosylerythritol lipid is not added in this comparative example, but 0.8 parts by weight of sorbitan sesquiisostearate is added.

[0080] Comparative Example 12

[0081] An essential oil. The composition and preparation method of the essential oil in this comparative example are the same as those of the ceramide-containing essential oil shown in Example 1, except that the total content of ceramide in this comparative example is 0.20 parts, and the weight parts of natural plant oils are 81.8 parts, including 29.9 parts of filbert oil, 21.9 parts of grape seed oil, 18 parts of wheat germ oil, 10 parts of oat kernel oil, and 2 parts of avocado tree fat.

[0082] Test Example 1: Stability Test

[0083] In this test example, the ceramide-containing essential oils shown in Examples 1 to 5 and the essential oils shown in Comparative Examples 1 to 11 were subjected to room temperature stability tests and low temperature stability tests. Specifically, each essential oil was placed at 25°C and 5°C for one month, and the appearance of the essential oil was observed. The test results are shown in Table 1.

[0084] Table 1

[0085]

[0086] Figure 1 The following is a comparison chart showing the results of the room temperature stability test of the essential oil of Example 1 and Comparative Example 1. Figure 1 As shown in Table 1, the method of adding an emulsifier to oil (Comparative Examples 9-11) cannot improve the stability of the essential oil containing ceramide. After being stored at room temperature and low temperature for one month, ceramide precipitation occurs, which is not conducive to the efficacy of ceramide. Further comparative analysis of Examples 1 to 5 with Comparative Examples 1 to 5 and Comparative Example 12 can confirm that in the technical solution of the present invention, by adding an appropriate amount of mannosylerythritol lipid to oil, not only the dissolution of ceramide in oil is promoted, but more importantly, the stability of ceramide in the prepared essential oil is enhanced. After being stored at room temperature and low temperature for one month, the essential oil containing ceramide of the present invention is still in a translucent state. By applying the essential oil of the present invention to human skin, the physiological function of ceramide can be fully exerted.

[0087] Test Example 2: Moisturizing performance test

[0088] In this test example, 35 female subjects aged 25 to 40 were selected to test the skin moisturizing properties of the ceramide-containing essence oil in Example 1. The subject exclusion criteria were those with obvious skin lesions or severe allergic reactions, those who had received skin treatment or beauty treatments (such as laser, injections, etc.) in the last 6 months, and those who used drugs that affected the skin condition.

[0089] Specifically, the stratum corneum moisture meter Corneometer was used to test the skin moisturizing performance of Example 1. The stratum corneum moisture content of the subjects after using the ceramide-containing essence oil of Example 1 for 7 days, 14 days and 28 days was tested respectively. The test was performed based on the capacitance principle. The larger the value, the higher the stratum corneum moisture content of the subject. The test results are shown in Table 1. Figure 2 As shown, it is verified that the application of the ceramide-containing essence oil of Example 1 has a significant skin moisturizing effect.

[0090] Test Example 3: Anti-inflammatory performance test

[0091] This test example uses the method of measuring the content of macrophage inflammatory factors in vitro in the cosmetic soothing efficacy test to evaluate the anti-inflammatory efficacy of the ceramide-containing essence oil in Example 1.

[0092] Bacterial lipopolysaccharide (LPS) binds to the surface antigen recognition receptor of mouse monocytic leukemia cells RAW264.7, inducing macrophages to release inflammatory mediator nitric oxide (NO). Excessive NO can promote macrophages to release inflammatory factors such as interleukin 6 (IL-6) and tumor necrosis factor (TNF-α), and these inflammatory factors can promote cells to secrete more NO, forming a vicious cycle and aggravating the inflammatory response. In this test example, the inflammatory factor inhibition rate of the sample group after administration is measured to evaluate the inhibitory effect of the sample on the release of inflammatory factors, thereby evaluating whether the sample has anti-inflammatory and soothing effects.

[0093] First, a cytotoxicity test was performed in this test case, and the results were as follows: Figure 3 As shown. Figure 3 It can be confirmed that the administration of the ceramide-containing essential oil of Example 1 at a concentration of 1% or less has no effect on the safety of macrophages.

[0094] Furthermore, in this test example, a model group (M) and a blank control group (BC) were set up to compare the anti-inflammatory properties of the ceramide-containing essential oil in Example 1. The results are as follows: Figure 4 As shown. Figure 4 It can be confirmed that the content of the in vitro inflammatory factor TNF-α in the positive control group (PC) using dexamethasone was significantly reduced compared with its model group (M) (P<0.05), and the coefficient of variation CV value of the OD value of each group was ≤20%, and the test system was effective; compared with the model group, the relative content of TNF-α in the sample group of Example 1 was significantly reduced (P<0.05), proving that the ceramide-containing essential oil of the present invention has anti-inflammatory and soothing effects.

[0095] Test Example 4: Repair Performance Test

[0096] In this test example, 35 female subjects aged 25 to 40 were selected to test the skin barrier repair effect of the ceramide-containing essence oil in Example 1. The exclusion criteria for the subjects were those with obvious skin lesions or severe allergic reactions, those who had received skin treatment or beauty projects (such as laser, injection, etc.) in the past 6 months, and those who used drugs that affected the skin condition.

[0097] Specifically, the TEWL of the skin in Example 1 was tested using a Tewameter. The open skin transepidermal water loss meter is based on Fick's diffusion law to evaluate the skin barrier function. The larger the value, the more serious the damage to the skin barrier function; the smaller the value, the better the skin barrier function. The skin TEWL of the subjects who used the ceramide-containing essence oil in Example 1 for 7 days, 14 days and 28 days was tested, and the test results were shown in Figure 1. Figure 5 As shown. Figure 5 It can be confirmed that the application of the ceramide-containing essence oil of Example 1 of the present invention can significantly reduce the skin TEWL, reflecting that the ceramide-containing essence oil of the present invention has a significant repair effect on the skin barrier.

[0098] It should be noted that the above contents are further detailed descriptions of the present invention in combination with specific implementation methods, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple improvements can be made without departing from the concept of the present invention, which should be regarded as falling within the scope of protection of the present invention.

Claims

1. A ceramide-containing essential oil, characterized in that: The ceramide-containing essential oil consists of ceramide, mannosylerythritol lipid, synthetic oil and natural plant oil.

2. The ceramide-containing essential oil according to claim 1, characterized in that In parts by weight, the ceramide-containing essential oil comprises: Ceramide 0.01-0.15 parts; Mannosylerythritol 0.05-2 parts; 1-25 parts of synthetic oil; the rest is natural plant oil.

3. The ceramide-containing essential oil according to claim 2, characterized in that In parts by weight, the ceramide-containing essential oil comprises 0.01-0.1 parts of ceramide, 0.1-1 parts of mannosylerythritol lipids, 3-18 parts of synthetic oils, and the remainder is natural plant oils.

4. The ceramide-containing essential oil according to claim 3, characterized in that In parts by weight, the ceramide-containing essential oil comprises 0.3-0.8 parts of mannosylerythritol lipid.

5. The ceramide-containing essential oil according to claim 1, characterized in that The ceramide includes one or more of ceramide AP, ceramide NP, ceramide NS, and cetyl-PG hydroxyethyl palmitamide.

6. The ceramide-containing essential oil according to claim 1, characterized in that The synthetic oil comprises one or both of triglyceride and monoester oil.

7. The ceramide-containing essential oil according to claim 6, characterized in that The triglyceride is selected from one or more of caprylic / capric triglyceride, caprylic / capric / succinic triglyceride, and tri(ethylhexanoin)glyceride.

8. The ceramide-containing essential oil according to claim 6, characterized in that The monoester oil is selected from one or more of coconut oil caprylate / caprate, ethylhexyl palmitate, isopropyl myristate, and isopropyl isostearate.

9. The ceramide-containing essential oil according to claim 1, characterized in that The natural plant oils include one or more of filbert oil, grape seed oil, wheat germ oil, safflower seed oil, sweet almond oil, rapeseed oil, oat kernel oil, avocado tree butter, spearmint leaf oil, cardamom seed oil, meadowfoam seed oil, low-erucic acid rapeseed oil, carrot seed oil, sunflower seed oil, and sea buckthorn seed oil.

10. The method for preparing the ceramide-containing essential oil according to any one of claims 1 to 9, characterized in that: include: (1) dissolving ceramide and mannosylerythritol lipid in synthetic oil in sequence at 85-95° C. to obtain a first material; (2) After cooling the first material to a temperature not higher than 50° C., adding natural plant oil and fat and mixing evenly to obtain the ceramide-containing essential oil.

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