Method for simultaneously and rapidly identifying desmodium and desmodium styracifolium

Abstract

The invention relates to a method for identifying lysimachia christinae hance and desmodium styracifolium, in particular to a method for simultaneously and rapidly identifying the lysimachia christinae hance and the desmodium styracifolium, and aims to solve the problems that the desmodium styracifolium and the desmodium styracifolium are easy to confuse and have many similarities in the aspects of characters, microscopic characteristics and the like, the character identification and microscopic identification processes are complicated and are not easy to observe, and the Therefore, the method is difficult to distinguish from characters and microscopes, and is not easy to operate. A liquid phase content detection and identification method is high in cost and long in time; in order to overcome the defects of difficulty in operation, tedious and complicated process and unstable detection result by adopting a thin-layer identification method, the method for simultaneously and quickly identifying the lysimachia christinae hance and the desmodium styracifolium comprises the steps of ultrasonic extraction, thin-layer sample application, developing by utilizing a developing solvent, developing and inspecting, and the lysimachia christinae hance and the desmodium styracifolium are directly identified and distinguished. Used materials are convenient to obtain, the process is simple, and the efficiency of thin-layer identification is effectively improved.

Description

Technical Field

[0001] The invention relates to a method for identifying Lysimachia chinensis and Lysimachia truncatum, and particularly relates to a method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia truncatum. Background Art

[0002] The Chinese Pharmacopoeia states that: Desmodium styracifolium is the dried whole herb of Lysimachiachristinae Hance, a plant of the Primulaceae family. It is harvested in summer and autumn, impurities are removed, and it is dried in the sun. This product has the effects of removing dampness and jaundice, promoting diuresis and relieving stranguria, detoxifying and reducing swelling. It is used for damp-heat jaundice, gallbladder distension and flank pain, stranguria with stones, stranguria with heat, painful and astringent urination, carbuncle, furuncle, snake and insect bites, etc. This product, calculated as a dry product, contains a total amount of quercetin and kaempferol (HPLC) of not less than 0.10%. Desmodium styracifolium is the dried aerial part of Desmodium styracifolium (Osb.) Merr., a plant of the Leguminosae family. It is harvested in summer and autumn, impurities are removed, and it is dried in the sun. This product has the effects of removing dampness and jaundice, promoting diuresis and relieving stranguria. It is used for jaundice, red urine, stranguria with heat, stranguria with stones, painful and astringent urination, edema, and oliguria. This product, calculated on the basis of dry product, contains not less than 0.13% schaftoside (HPLC).

[0003] Although the names of Herba Lysimachiae and Herba Lysimachiae are similar and both are diuretics and anti-jaundice drugs, their sources, properties, flavors, meridians, usage and dosage are different, and they are easy to confuse. Modern research shows that Herba Lysimachiae mainly contains saponins, flavonoid glycosides, alkaloids, tristris, stearic acid, β-sitosterol, polysaccharides, etc. Herba Lysimachiae contains phenolic components and sterols, flavonoids, amino acids, tannins, volatile oils, choline, potassium salts and other ingredients. At present, the methods for identifying Herba Lysimachiae and Herba Lysimachiae include property identification, microscopic identification, thin layer identification and liquid phase identification. There are many similarities between Herba Lysimachiae and Herba Lysimachiae in properties and microscopic characteristics. The process of property identification and microscopic identification is complicated and difficult to observe. Therefore, it is difficult to distinguish them from properties and microscopy, and it is not easy to operate; while the detection and identification of liquid phase content is costly and time-consuming, which is not conducive to rapid identification.

[0004] The thin layer identification method of Lysimachia in the Chinese Pharmacopoeia is as follows: take 1g of the powder of this product, add 50ml of 80% methanol, heat and reflux for 1 hour, cool, filter, evaporate the filtrate, add 10ml of water to the residue to dissolve, shake and extract twice with ether, 10ml each time, discard the ether solution, add 10ml of dilute hydrochloric acid to the water solution, heat in a water bath for 1 hour, take out, cool quickly, shake and extract twice with ethyl acetate, 20ml each time, combine the ethyl acetate solution, wash with 30ml of water, discard the water solution, evaporate the ethyl acetate solution, and add 1ml of methanol to the residue to dissolve as the test solution. Separately take the quercetin reference substance and kaempferol reference substance, add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. According to the thin layer chromatography method (General Rule 0502), 5 μl of the test solution and 2 μl of the reference solution are respectively spotted on the same silica gel G thin layer plate, and toluene-ethyl formate-formic acid (10:8:1) is used as the developing agent. After development, the plate is taken out, dried, sprayed with 3% aluminum chloride ethanol solution, heated at 105°C for several minutes, and examined under ultraviolet light (365nm). In the chromatogram of the test sample, a fluorescent spot of the same color appears at the corresponding position of the chromatogram of the reference.

[0005] The thin layer identification method of Desmodium truncatum in the Chinese Pharmacopoeia is as follows: Take 0.2g of the powder of this product, add 25ml of 80% methanol, ultrasonically extract for 20 minutes, filter, evaporate the filtrate, and add 10ml of 50% methanol to dissolve the residue as the test solution. Take another 0.2g of Desmodium truncatum reference medicinal material and prepare the reference medicinal material solution in the same way. Take the schaftoside reference substance and add 50% methanol to prepare a solution containing 75μg per 1ml as the reference substance solution. According to the thin layer chromatography method (General Rule 0502), take 1μl of each of the above three solutions and spot them on the same polyamide film respectively, use ethyl acetate-butanone-formic acid (5:1:1) as the developing agent, develop, take out, dry, spray with aluminum chloride test solution, blow dry with hot air, and examine under ultraviolet light (365nm). In the chromatogram of the test product, fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the reference medicinal material and the chromatogram of the reference substance.

[0006] The thin-layer identification method of Lysimachia in the Chinese Pharmacopoeia uses quercetin and kaempferol. Both components need to be hydrolyzed with hydrochloric acid and extracted repeatedly before they can be detected. Therefore, the sample pretreatment is very cumbersome, the test results are very unstable, and the error is large. Quercetin and kaempferol are ubiquitous in most plants and basically have no specificity, which also increases the false positive results. The thin-layer identification method of Lysimachia in the Chinese Pharmacopoeia uses polyamide film and requires special development equipment. This detection method is outdated and difficult to operate, and it is difficult to adapt to the development requirements of modern thin-layer detection.

[0007] Thin layer identification methods usually have the advantages of low cost, simple operation, speed, and rich chromatographic information. However, after specific verification, it was found that the above-mentioned thin layer identification method requires the identification of Lysimachia sibiricum and Lysimachia truncatum separately, and cannot quickly, intuitively, and efficiently distinguish between Lysimachia sibiricum and Lysimachia truncatum. It is also difficult to operate, the process is cumbersome and complicated, and the test results are unstable, which is not conducive to practical application. Summary of the invention

[0008] The invention aims to solve the problems that Lysimachia strychnifolia and Lysimachia strychnifolia are easily confused, have many similarities in properties and microscopic characteristics, and the properties identification and microscopic identification processes are complicated and difficult to observe, so it is difficult to distinguish them from properties and microscopy, and it is not easy to operate; the detection and identification method using liquid phase content has high cost and long time; the thin layer identification method has the shortcomings of being difficult to operate, complicated process and unstable detection result, and provides a method for simultaneously and quickly identifying Lysimachia strychnifolia and Lysimachia strychnifolia.

[0009] The inventive concept of the present invention is to establish a simple and rapid method for identifying Lysimachia chinensis and Lysimachia chinensis based on the difference in schaftoside components between Lysimachia chinensis and Lysimachia chinensis, and to distinguish the two well based on the unique spots displayed on the thin layers of Lysimachia chinensis and Lysimachia chinensis.

[0010] To achieve the above objectives, the technical solutions provided by the present invention are as follows:

[0011] A method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia grandiflora, which is special in that it comprises the following steps:

[0012] Step 1, taking equal amounts of Herba Lysimachiae control samples and Herba Lysimachiae control samples, and the raw material sample to be tested, adding 70 vol% ethanol of 5 to 10 times the mass thereof, respectively, ultrasonically extracting for 30 to 60 minutes, filtering after cooling to room temperature, and collecting the filtrate to obtain Herba Lysimachiae sample solution, Herba Lysimachiae sample solution, and the raw material sample solution to be tested;

[0013] Step 2, spotting the Herba Lysimachiae sample solution, the Herba Lysimachiae sample solution and the raw material sample solution obtained in step 1 on a thin layer plate respectively;

[0014] Step 3, add the developing agent into the developing tank and pre-saturate for 15 to 20 minutes;

[0015] The developing agent is a mixed solution of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of (9-11): (0.5-1.5): (0.5-1.5): (1-3);

[0016] Step 4, placing the thin layer plate in step 2 into the developing cylinder, the developing agent rises along the thin layer plate, and when the front edge of the developing agent rises to the upper edge of the thin layer plate and is 6 cm to 10 cm away from the sample origin, the thin layer plate is taken out;

[0017] Step 5, after drying the thin layer plate in step 4, spraying a color developer onto the thin layer plate, heating it at 105° C. for 3 to 5 minutes, and after cooling, inspecting it at a wavelength of 365 nm to obtain a corresponding thin layer chromatogram; the color developer is 5 vol% to 10 vol% ethanol sulfuric acid;

[0018] Step 6, comparing the thin layer chromatogram of the raw material sample to be tested with the thin layer chromatograms of the Herba Lysimachiae control samples and the Herba Lysimachiae control samples, respectively, to determine the type of the raw material sample to be tested, and to complete the identification of Herba Lysimachiae and Herba Lysimachiae.

[0019] Furthermore, in step 3, the expansion cylinder is a double-slot expansion cylinder, and the expansion doses in the double slots of the double-slot expansion cylinder are consistent.

[0020] Furthermore, in step 4, placing the thin layer plate of step 2 into the developing cylinder specifically comprises: placing the thin layer plate obliquely in the developing cylinder, and the liquid level of the developing agent is lower than the origin height of the thin layer spotting.

[0021] Furthermore, in step 1, the amount of ethanol used is 6 times the amount, and the ultrasonic extraction is performed for 60 minutes;

[0022] In step 2, the thin layer plate is a silica gel GF254 thin layer plate or a silica gel G60 thin layer plate;

[0023] In step 3, the pre-saturation time is 15 minutes; the developing agent is a mixed solution of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of 10:1:1:2;

[0024] In step 4, when the distance from the sampling origin is 8 cm, the thin layer plate is taken out;

[0025] In step 5, the color developing agent is 5 vol% ethanolic sulfuric acid.

[0026] Beneficial effects of the present invention:

[0027] 1. The present invention uses ethanol to directly perform ultrasonic extraction and filtration on the sample to be tested, which has simple pretreatment of the sample to be tested, low cost, and easy operation, thereby avoiding the problem of complex pretreatment of the thin layer identification method of Lysimachia sibiricum in the existing method, which easily leads to unstable test results.

[0028] 2. The developing agent of the present invention is ethyl acetate, glacial acetic acid, formic acid and water mixed in a certain volume ratio, the obtained thin layer chromatography has good spot separation effect, the characteristic spots of each sample are prominent, and there is no spot tailing and spot diffusion phenomenon, the results are obviously different, easy to judge, no secondary identification is required, and the identification of Lysimachia sibiricum and Lysimachia sibiricum is achieved at the same time, avoiding the deficiency that Lysimachia sibiricum and Lysimachia sibiricum cannot be distinguished and identified at one time in the existing method; at the same time, the present invention adopts 5% to 10% sulfuric acid ethanol for color development, combined with the above-mentioned developing agent, the obtained thin layer spots are many and the spot colors are obvious, the thin layer chromatogram is clearly contrasted, the results are more referenceable, and the operation is simple and fast, avoiding the deficiency that the aluminum chloride used in the existing method only develops the color of flavonoid components and has few spots.

[0029] 3. The thin layer plate of the present invention is a GF254 silica gel thin layer plate, which is easy to obtain and simple to use, thereby improving the efficiency of thin layer identification.

[0030] 4. The spots of Lysimachia chinensis and Lysimachia grandiflora in the thin layer chromatogram of the present invention are clearly distinguishable, the results are intuitive, easy to identify and distinguish, the results are intuitive and reliable, the operation process is simple and efficient, the results are highly sensitive and specific, and the reagents and materials used are easy to obtain and low in cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a thin layer chromatogram obtained in Example 1 of the present invention; wherein, sample No. S is a schaftoside reference sample (China Inspection Institute, 111912-202204, purity 94.9%), sample No. 1 is a Herba Lysimachiae reference sample, sample No. 2 is a Herba Lysimachiae control sample, sample No. 3 is a Herba Lysimachiae raw material, and sample No. 4 is a Herba Lysimachiae raw material;

[0032] Figure 2 This is a thin layer chromatogram obtained by using the thin layer identification method of Desmodium strychnifolium in the Chinese Pharmacopoeia;

[0033] Figure 3 It is a thin layer chromatogram obtained by using the thin layer identification method of Lysimachia chinensis in the "Chinese Pharmacopoeia"; among them, sample S1 is a quercetin control sample (China National Institute for Food and Drug Control, 111912-202204, purity 98%), sample S2 is a kaempferol control sample (China National Institute for Food and Drug Control, 110861-202214, purity 97.4%), sample 1 is a Lysimachia chinensis control sample, sample 2 is a Lysimachia sibiricum control sample, sample 3 is a Lysimachia chinensis raw material, and sample 4 is a Lysimachia sibiricum raw material. DETAILED DESCRIPTION

[0034] Embodiment 1

[0035] The method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia grandiflora of the present invention specifically comprises the following steps:

[0036] 1. Ultrasonic Extraction

[0037] Take equal amounts of Herba Lysimachiae control samples, Herba Lysimachiae control samples and raw material samples to be tested as samples to be tested, respectively, use 70 vol% ethanol in an amount 5 to 10 times the mass of the samples to be tested in an ultrasonic extraction for 30 to 60 minutes, take out and put to room temperature, use a microporous filter membrane to filter, and collect the filtrate to obtain Herba Lysimachiae sample solution, Herba Lysimachiae sample solution and raw material sample solution to be tested, respectively.

[0038] In this embodiment, the Herba Lysimachiae control sample is the Herba Lysimachiae control sample (121187-202104) from China National Institute for Food and Drug Control, and the Herba Lysimachiae control sample is the Herba Lysimachiae control sample (121248-202006) from China National Institute for Food and Drug Control. Among them, the raw material samples to be tested include Herba Lysimachiae raw materials and Herba Lysimachiae raw materials purchased on the market. For the convenience of extraction, the sampling amount of the samples to be tested is 0.2 g, and they are placed in stoppered conical flasks respectively; the amount of ethanol used is 6 times the mass of the sample to be tested, and ultrasonic extraction is performed for 60 minutes.

[0039] 2. Thin layer spotting

[0040] The three sample solutions were spotted on the same silica gel GF254 thin layer plate at different positions on the same horizontal line. The spotting position was about 1.0 to 1.5 cm away from the lower edge of the silica gel GF254 thin layer plate.

[0041] In this embodiment, the distance between the spotting origin and the lower edge of the silica gel GF254 thin layer plate is 1.0 cm, and the three sample solutions are spotted on the same silica gel GF254 thin layer plate to ensure the consistency of the development conditions.

[0042] In other embodiments of the present invention, a silica gel G60 thin layer plate may also be used.

[0043] 3. Prepare the developing agent

[0044] Place an appropriate amount of developing agent in a double-slot developing cylinder. The specifications of the developing cylinder can be 10×10cm, 20×10cm, or 10×20cm, and the developing agent dosage in the double slots should be kept consistent. Seal the developing cylinder and let it stand for 15 to 20 minutes to pre-saturate the developing agent.

[0045] In the present invention, the developing agent is a mixed solution of ethyl acetate, glacial acetic acid, formic acid and water, and the volume ratio is (9-11): (0.5-1.5): (0.5-1.5): (1-3).

[0046] In this embodiment, the volume ratio of ethyl acetate-glacial acetic acid-formic acid-water is 10:1:1:2, and a total of 20 mL of developing agent is prepared and added to a double-slot developing cylinder with a specification of 10×10 cm. The developing amount in the two slots is consistent, the double-slot developing cylinder is sealed, and the double-slot developing cylinder is pre-saturated with the developing agent for 15 minutes.

[0047] 4. Expand

[0048] Open the pre-saturated developing cylinder and quickly place the thin layer plate in it. The thin layer plate should be tilted against the developing cylinder, and the developing agent in the developing cylinder should not cover the sample spotting position. The developing agent rises along the thin layer plate. When the front edge of the developing agent rises to the upper edge of the thin layer plate, it is 8cm to 10cm away from the sample spotting position.

[0049] In this embodiment, the thin layer plate is taken out when the front edge of the developing agent moves upward to 8 cm away from the sample spotting position.

[0050] 5. Color display and inspection

[0051] After the thin layer plate is taken out and dried, 5 vol% to 10 vol% sulfuric acid ethanol color developer is sprayed on the developed thin layer plate, and then it is placed on an electric heating plate, heated at 105°C for 3 to 5 minutes, cooled to room temperature, and inspected at a wavelength of 365nm.

[0052] In this embodiment, the developing agent on the thin layer plate was dried with a hair dryer, and 5 vol% sulfuric acid ethanol color developing agent was sprayed on the thin layer plate, which was placed on an electric heating plate and heated at 105°C for 3 minutes for reaction. After cooling to room temperature, the plate was examined under ultraviolet light at a wavelength of 365 nm to obtain the corresponding thin layer chromatogram, as shown in FIG. Figure 1 shown.

[0053] 6. Experimental Results

[0054] In this example, 10 mg of schaftoside control sample was weighed and dissolved in 10 ml of methanol to obtain a 1 mg / mL quercetin methanol solution, which was then treated by steps 2 to 5 and examined under ultraviolet light at a wavelength of 365 nm to obtain a corresponding thin layer chromatogram. Figure 1 By comparing sample S in the experiment with samples 1-4, it can be seen that there are no spots corresponding to schaftoside on the thin layer chromatograms of samples 1 and 3, which proves that the samples contain Lysimachia chinensis, and the thin layer chromatogram of Lysimachia strychnifolia has spots at Rf≈0.15, 0.27, 0.30, and 0.55, while there are no spots at the corresponding positions of Lysimachia strychnifolia. It can be seen that after the ultrasonic extraction, development, and color development in the above steps, under ultraviolet light with a wavelength of 365nm, the spots on the thin layer chromatograms of Lysimachia strychnifolia and Lysimachia strychnifolia are obviously different.

[0055] Figure 2 In order to identify the above-mentioned samples using the thin layer identification method of Desmodium Styracifolium in the "Chinese Pharmacopoeia", according to the results, Desmodium Styracifolium also shows corresponding spots of schaftoside, but this method uses a polyamide film plate, the method is outdated, the operation is complicated, and other spots are blurred, which is not conducive to accurate identification of the two components.

[0056] Figure 3The thin layer chromatograms of the above-mentioned samples to be tested are identified by the thin layer identification method of Lysimachia chinensis in the Chinese Pharmacopoeia. In addition, 10 mg of quercetin control sample was dissolved in 10 ml of methanol solution to obtain 1 mg / mL quercetin methanol solution. The same method was used to prepare 1 mg / mL kaempferol methanol solution. The quercetin control sample and kaempferol control substance were tested by this method. It can be seen that this method uses quercetin and kaempferol to distinguish Lysimachia chinensis from Lysimachia sibiricum, but the pretreatment of this method is complicated. In addition to the quercetin and kaempferol control substance spots, there are almost no other spots, and quercetin and kaempferol are not very characteristic. Most plants have these two spots. The judgment basis is too single and not specific, and it is not possible to identify Lysimachia chinensis from Lysimachia sibiricum well. Therefore, by comparison Figure 2 and Figure 3 It can be seen that the thin layer chromatogram obtained by the present invention has clear spots, obvious distinction, reliable and stable results, and strong specificity.

[0057] Embodiment 2

[0058] The method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia grandiflora of the present invention is specifically as follows:

[0059] Take 0.2 g of the Herba Lysimachiae control sample, the Herba Lysimachiae control sample, the Herba Lysimachiae raw material and the Herba Lysimachiae raw material respectively, the sources of the materials are the same as in Example 1, place them in stoppered conical flasks respectively, and add 70 vol% ethanol in an amount 6 times of its mass respectively, ultrasonically extract for 60 minutes, take out and put it at room temperature, filter with a microporous filter membrane, collect the filtrate as the sample solution, and spot the sample solutions on the silica gel GF254 thin layer plate respectively, and the distance between the spotting origin and the lower edge of the silica gel GF254 thin layer plate is 1.0 cm.

[0060] Prepare 20 mL of developing agent, which is composed of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of 9:0.5:0.5:1. Place the developing agent in a 10×10 cm double-slot developing cylinder, with the amount of developing agent in the two slots being the same. Seal the developing cylinder and pre-saturate the developing cylinder with the developing agent for 15 minutes.

[0061] Quickly place the silica gel GF254 thin layer plate into the saturated developing cylinder, so that the silica gel GF254 thin layer plate is tilted against the developing cylinder, and the developing agent in the developing cylinder cannot submerge the sample origin. The developing agent develops upward along the silica gel GF254 thin layer plate, and when the leading edge of the developing agent is 8 cm from the sample origin, take out the silica gel GF254 thin layer plate.

[0062] Use a hair dryer to dry the developing agent on the silica gel GF254 thin layer plate, spray 10 vol% sulfuric acid ethanol color developer on the silica gel GF254 thin layer plate, place it on an electric heating plate, and heat it at 105°C for 5 minutes. Let it cool to room temperature, examine it under ultraviolet light with a wavelength of 365nm, and obtain the corresponding thin layer chromatogram, and compare it to obtain the type of raw materials.

[0063] Embodiment 3

[0064] The method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia grandiflora of the present invention is specifically as follows:

[0065] Take 0.2g of Herba Lysimachiae, Herba Lysimachiae control samples and Herba Lysimachiae and Herba Lysimachiae raw materials purchased from the market, respectively, put them in a stoppered conical flask, add 6 times the amount of 70vol% ethanol, ultrasonically extract for 60 minutes, take out and let it cool to room temperature, filter with a microporous filter membrane, and use the filtrate as the sample solution. The sample solutions are respectively spotted on a silica gel GF254 thin layer plate, and the distance between the spot origin and the lower edge of the silica gel GF254 thin layer plate is 1.0cm.

[0066] Prepare 20 mL of developing agent, which is composed of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of 11:1.5:1.5:3, and place the developing agent in a 10×10 cm double-slot developing cylinder. The amount of developing agent in the two slots should be the same. Seal the developing cylinder and pre-saturate the developing cylinder with the developing agent for 15 minutes.

[0067] Quickly place the silica gel GF254 thin layer plate into the saturated developing cylinder, so that the silica gel GF254 thin layer plate is tilted against the developing cylinder, and the developing agent in the developing cylinder cannot submerge the sample origin. The developing agent develops upward along the silica gel GF254 thin layer plate, and when the leading edge of the developing agent is 8 cm from the sample origin, take out the silica gel GF254 thin layer plate.

[0068] Use a hair dryer to dry the developing agent on the silica gel GF254 thin layer plate, spray 8 vol% sulfuric acid ethanol color developer on the silica gel GF254 thin layer plate, place it on an electric heating plate, and heat it at 105°C for 3 minutes. Let it cool to room temperature, examine it under ultraviolet light with a wavelength of 365nm, and obtain the corresponding thin layer chromatogram, and compare it to obtain the type of raw materials.

Claims

1. A method for simultaneously and quickly identifying Lysimachia chinensis and Lysimachia grandiflora, characterized in that: The following steps are involved: Step 1, taking equal amounts of Herba Lysimachiae control samples and Herba Lysimachiae control samples, and the raw material sample to be tested, adding 70 vol% ethanol of 5 to 10 times the mass thereof, respectively, ultrasonically extracting for 30 to 60 minutes, filtering after cooling to room temperature, and collecting the filtrate to obtain Herba Lysimachiae sample solution, Herba Lysimachiae sample solution, and the raw material sample solution to be tested; Step 2, spotting the Herba Lysimachiae sample solution, the Herba Lysimachiae sample solution and the raw material sample solution obtained in step 1 on a thin layer plate respectively; Step 3, add the developing agent into the developing tank and pre-saturate for 15 to 20 minutes; The developing agent is a mixed solution of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of (9-11): (0.5-1.5): (0.5-1.5): (1-3); Step 4, placing the thin layer plate in step 2 into the developing cylinder, the developing agent rises along the thin layer plate, and when the front edge of the developing agent rises to the upper edge of the thin layer plate and is 6 cm to 10 cm away from the sample origin, the thin layer plate is taken out; Step 5, after drying the thin layer plate in step 4, spraying a color developer onto the thin layer plate, heating it at 105° C. for 3 to 5 minutes, and after cooling, inspecting it at a wavelength of 365 nm to obtain a corresponding thin layer chromatogram; the color developer is 5 vol% to 10 vol% ethanol sulfuric acid; Step 6, comparing the thin layer chromatogram of the raw material sample to be tested with the thin layer chromatograms of the Herba Lysimachiae control samples and the Herba Lysimachiae control samples, respectively, to determine the type of the raw material sample to be tested, and to complete the identification of Herba Lysimachiae and Herba Lysimachiae.

2. A method for simultaneously and rapidly identifying Lysimachia chinensis and Lysimachia grandiflora according to claim 1, characterized in that: In step 3, the expansion cylinder is a double-slot expansion cylinder, and the expansion doses in the double slots of the double-slot expansion cylinder are consistent.

3. A method for simultaneously and rapidly identifying Lysimachia chinensis and Lysimachia grandiflora according to claim 2, characterized in that: In step 4, placing the thin layer plate in step 2 into the developing cylinder specifically includes: placing the thin layer plate obliquely in the developing cylinder, and the liquid level of the developing agent is lower than the origin height of the thin layer spotting.

4. A method for simultaneously and rapidly identifying Lysimachia sibiricum and Lysimachia luxuriantii according to any one of claims 1 to 3, characterized in that: In step 1, the amount of ethanol used was 6 times the amount, and ultrasonic extraction was performed for 60 minutes; In step 2, the thin layer plate is a silica gel GF254 thin layer plate or a silica gel G60 thin layer plate; In step 3, the pre-saturation time is 15 minutes; the developing agent is a mixed solution of ethyl acetate, glacial acetic acid, formic acid and water in a volume ratio of 10:1:1:2; In step 4, when the distance from the sampling origin is 8 cm, the thin layer plate is taken out; In step 5, the color developing agent is 5 vol% ethanolic sulfuric acid.

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