Method for preventing and treating potato verticillium wilt by using ABT rooting powder

Abstract

The invention provides a method for preventing and treating potato verticillium wilt by using ABT rooting powder, which comprises the following steps: fully dissolving the ABT rooting powder in alcohol, and diluting with distilled water for later use; the method comprises the following steps: taking out test-tube plantlets of Desiree and Zhongshu 19 at the seedling age of 2 weeks, cutting off root systems, soaking potato stem bases in ABT rooting powder, planting the potato stem bases in a water culture box, observing the growth condition of the potato root systems, and screening the ABT rooting powder with proper concentration for later use; planting Desiree and Zhongshu 19 test-tube plantlets, preparing a non-alfalfa verticillium Vn011 spore suspension when the plant grows to 4-6 true leaves, and irrigating the non-alfalfa verticillium Vn011 spore suspension to the base part of a potato stem; 3 days after the pathogenic bacteria are inoculated, ABT rooting powder is poured to the base parts of potato stems; after inoculating the pathogenic bacteria for 20 days, 30 days and 40 days, counting the disease grade, and calculating the disease index; harvesting the potatoes 50 days after inoculating the pathogenic bacteria, measuring the yield, and observing and counting the root growth condition of the potatoes. The ABT rooting powder is utilized to promote rapid growth of potato root systems and plants, and the purpose of preventing and treating potato verticillium wilt is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of biological control of potato Verticillium wilt, and in particular to a method for controlling potato Verticillium wilt by using ABT rooting powder. Background Art

[0002] Potato is the fourth largest food crop in my country and is of strategic significance for ensuring national food security and promoting regional economic development. In recent years, my country's potato planting area has continued to increase. Due to limited arable land and continuous cropping, the soil-borne disease Verticillium wilt caused by Verticillium fungi has become increasingly serious, affecting potato production in major production areas in northern my country to varying degrees.

[0003] Verticillium dahliae and Verticillium nonalfalfae are the main pathogens that cause potato Verticillium wilt. Verticillium wilt can survive in the soil for more than ten years in dormant forms such as microsclerotia or dormant hyphae. When conditions are suitable, it germinates into mycelium and invades the host root vascular bundle from the root, causing systemic infection.

[0004] Potato Verticillium wilt is also known as "early death disease". After the plant is infected, it usually develops from the bottom to the top. The lower leaves of the plant first show symptoms. Sometimes only one main stem or the leaves on one side of the stem wilt first. In the early stage of the disease, chlorotic yellow spots appear along the leaf edge and between the main veins at the tip of the diseased leaf, and gradually deepen, but the main vein and the leaf flesh near it remain green, resembling a "watermelon rind". As the disease worsens, the entire leaf turns from yellow to brown, and finally, the entire compound leaf dries up and does not fall off. In the late stage of the disease, the vascular tissue of the main stem turns brown, causing the entire potato plant to wilt and die prematurely. The disease of underground tubers begins at the junction of the stolon and the tuber. When the diseased potato is cross-cut, the brown vascular bundle is clearly visible. The harm of Verticillium wilt to potato plants can cause the growth of the plant to be stunted, premature death, and reduced production at the mildest; and cause the tubers to turn brown at the worst, causing a significant reduction in production while reducing the commercial attributes of potatoes.

[0005] At present, the prevention and control methods of potato wilt are mainly chemical control and biological control measures. Chemical control-related methyl bromide, methyl isothiocyanate, etc. are widely used as soil fumigants for fumigation before crop planting. However, excessive use of chemical pesticides has a serious impact on the ecological environment. Bacterial agents related to biological control, such as Bacillus, Pseudomonas, Trichoderma harzianum and other biocontrol bacteria, are also widely used, but their effects in the field are not obvious or the effects are unstable in different regions, and the environmental safety risks brought by biological control are still unclear.

[0006] ABT rooting powder is an important type of auxin growth regulator, mainly containing two active ingredients, indoleacetic acid (IAA) and naphthaleneacetic acid (NAA). IAA plays an important role in the division, elongation, differentiation and seed germination of plant cells, root development and nutritional growth process. NAA is a broad-spectrum plant growth regulator that can rapidly promote cell division and expansion, induce the formation of adventitious roots, and thus has a significant effect of promoting growth and promoting rooting. The inventors of the present application have found through research that there is currently no report on the use of ABT rooting powder to prevent potato wilt. Summary of the invention

[0007] In view of the technical problems of high cost and environmental safety caused by the existing chemical control and biological control of potato wilt, the present invention provides a method for controlling potato wilt using ABT rooting powder. The method uses ABT rooting powder to promote the rapid growth of potato roots and plants, thereby achieving the purpose of controlling potato wilt.

[0008] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0009] A method for preventing and controlling potato Verticillium wilt by using ABT rooting powder, comprising:

[0010] 1) Screening of ABT rooting powder concentration: 1 g ABT rooting powder was added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to a concentration of 0 mg / L to 80 mg / L, sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings were taken out, the roots were cut off, and the base of the potato stem was soaked in ABT rooting powder with a concentration of 0 mg / L to 80 mg / L for 20 minutes, and then the potato test tube seedlings were planted in a hydroponic box. After 7 days, the growth of the potato root system was observed, and the ABT rooting powder with a concentration of 5 mg / L to 40 mg / L was screened for use;

[0011] 2) ABT rooting powder to prevent and control potato wilt:

[0012] 21) Inoculation of potato plants with Verticillium non-lucerne: Two-week-old Désirée and Zhongshu 19 test tube seedlings were planted in pots. When the potatoes grew to 4 to 6 true leaves, i.e. 2 to 3 weeks after planting, a concentration of 1×10 7 80 mL of the non-alfactoid Verticillium medicaginii Vn011 spore suspension with cfu / mL was poured on the base of potato stems, and an equal amount of distilled water was used to inoculate the control plants;

[0013] 22) ABT rooting powder treatment: 3 days after inoculation with non-alfalfa Verticillium sphaeroides Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., ABT rooting powder with a concentration of 0 mg / L and 5 mg / L-40 mg / L, was respectively measured and poured on the base of the potato stem; 20, 30, and 40 days after inoculation with the pathogen, the disease level was counted and the disease index was calculated; 50 days after inoculation with the pathogen, the stem segments at the base of the potato stem were taken for pathogen isolation, the potatoes were harvested and the yield was measured, and the growth of the potato root system was observed and counted.

[0014] Furthermore, the ABT rooting powder added in step 1) was purchased from Beijing ABT Biotechnology Co., Ltd.

[0015] Further, in the step 1), 1 g of ABT rooting powder is added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to concentrations of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L, respectively, and sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings are taken out, the roots are cut off, and the base of the potato stem is soaked in distilled water, that is, 0 mg / L and 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L ABT rooting powder for 20 minutes, and then the potato test tube seedlings are planted in a hydroponic box, and the growth of the potato root system is observed after 7 days, and finally, the ABT rooting powder with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L is selected for use;

[0016] In the step 22), 3 days after the inoculation of non-alfalfa Verticillium Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., ABT rooting powder with a concentration of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L, was respectively measured and poured on the base of the potato stem.

[0017] Furthermore, in the step 22), 3 days after the inoculation of the pathogenic bacteria, 100 mL of 10 mg / L ABT rooting powder was measured and poured on the base of the potato stem.

[0018] Further, in the step 22), compared with the treatment with distilled water, i.e., the treatment with a concentration of 0 mg / L, the root length, root volume, root surface area, and root tip number of Desirée under the treatment with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L increased by 93.28% to 290.03%, 43.06% to 336.87%, 78.05% to 315.69%, and 46.44% to 152.22%, respectively; the root length, root volume, root surface area, and root tip number of Zhongshu 19 increased by 29.21% to 173.94%, 49.78% to 345.96%, 232.76% to 578.99%, and 69.49% to 388.54%, respectively.

[0019] Compared with the prior art, the method for preventing and controlling potato Verticillium wilt by using ABT rooting powder provided by the present invention comprises the following steps: watering the ABT rooting powder of different concentrations screened out after dilution on the base of potato stems inoculated with non-alfalfa Verticillium wilt Vn011, and comprehensively analyzing the disease severity of the aboveground part of the potato and the data on the root system and yield of the underground part, indicating that ABT rooting powder treatment can promote the growth and development of potato roots, delay the progression of Verticillium wilt, delay the time of plant death, and reduce the yield loss caused by premature plant death. Among them, when the concentration of ABT rooting powder is 5mg / L~40mg / L, it can significantly promote the growth of potato roots and plants; when the treatment concentration of ABT rooting powder is 10mg / L, the disease resistance effect is the best. Indicators such as disease grade, disease index and root growth show that this concentration can significantly enhance the resistance of potato to Verticillium wilt and delay the death of plants; when the treatment concentration of ABT rooting powder is 10mg / L, the yield loss is the lowest. The yield reduction rates of the medium-resistant variety Zhongshu 19 and the susceptible variety Désirée are 31.30% and 34.36% respectively, which significantly reduces the yield loss and prevents and controls potato Verticillium wilt. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of potato phenotypes after being treated with different concentrations of ABT rooting powder provided by the present invention.

[0021] Figure 2 It is a schematic diagram of the effect of different concentrations of ABT rooting powder provided by the present invention on preventing and treating potato Verticillium wilt.

[0022] Figure 3 It is a schematic diagram of the disease grade ratio and disease index of potato Verticillium wilt after being treated with different concentrations of ABT rooting powder provided by the present invention.

[0023] Figure 4 It is a schematic diagram of the potato root phenotype and data analysis after being treated with different concentrations of ABT rooting powder provided by the present invention.

[0024] Figure 5It is a schematic diagram of potato yield after being treated with different concentrations of ABT rooting powder provided by the present invention. DETAILED DESCRIPTION

[0025] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below with reference to specific diagrams.

[0026] The present invention provides a method for preventing and treating potato Verticillium wilt by using ABT rooting powder, comprising:

[0027] 1) Screening of ABT rooting powder concentration: 1 g ABT rooting powder was added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to a concentration of 0 mg / L to 80 mg / L, sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings were taken out, the roots were cut off, and the base of the potato stem was soaked in ABT rooting powder with a concentration of 0 mg / L to 80 mg / L for 20 minutes, and then the potato test tube seedlings were planted in a hydroponic box. After 7 days, the growth of the potato root system was observed, and the ABT rooting powder with a concentration of 5 mg / L to 40 mg / L was screened for use;

[0028] 2) ABT rooting powder to prevent and control potato wilt:

[0029] 21) Inoculation of potato plants with Verticillium non-lucerne: Two-week-old Désirée and Zhongshu 19 test tube seedlings were planted in pots. When the potatoes grew to 4 to 6 true leaves, i.e. 2 to 3 weeks after planting, a concentration of 1×10 7 80 mL of the non-alfactoid Verticillium medicaginii Vn011 spore suspension with cfu / mL was poured on the base of potato stems, and an equal amount of distilled water was used to inoculate the control plants;

[0030] 22) ABT rooting powder treatment: 3 days after inoculation with non-alfalfa Verticillium sphaeroides Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., ABT rooting powder with a concentration of 0 mg / L and 5 mg / L-40 mg / L, was respectively measured and poured on the base of the potato stem; 20, 30, and 40 days after inoculation with the pathogen, the disease level was counted and the disease index was calculated; 50 days after inoculation with the pathogen, the stem segments at the base of the potato stem were taken for pathogen isolation, the potatoes were harvested and the yield was measured, and the growth of the potato root system was observed and counted.

[0031] As a specific embodiment, the ABT rooting powder added in the step 1) is purchased from Beijing Abidi Biotechnology Co., Ltd., and specifically, ABT rooting powder No. 1 produced by the company can be used.

[0032] As a specific example, in the step 1), 1 g of ABT rooting powder is added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to concentrations of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L, respectively, and sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings are taken out, the roots are cut off, and the base of the potato stem is soaked in distilled water, that is, ABT rooting powder with a concentration of 0 mg / L and a concentration of 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L for 20 minutes, and then the potato test tube seedlings are planted in a hydroponic box, and the growth of the potato root system is observed after 7 days. By comparing the growth and development phenotypes of the susceptible variety Désirée and the medium-resistant variety Zhongshu 19 hydroponic seedlings treated with different concentrations of ABT rooting powder, the results showed that under the treatment of 5mg / L to 40mg / L concentration, the higher the concentration of ABT rooting powder, the more vigorous the potato plants grew and the more developed the root system was. The best treatment was 10mg / L ABT rooting powder. As the concentration of ABT rooting powder increased, the growth of the plants slowed down. When the concentration of ABT rooting powder reached 60mg / L, the growth of the plants was significantly inhibited. Figure 1 A-1B. In the figure, A represents the phenotype of the Désirée plant, B represents the phenotype of the Zhongshu 19 plant, 0-80 mg / L represents the treatment with 0-80 mg / L ABT rooting powder, scale = 10 cm, and finally the ABT rooting powders with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L were selected for use.

[0033] As a specific example, in the step 22), 3 days after the inoculation of non-alfalfa Verticillium Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., a concentration of 0 mg / L, and ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L were measured and watered on the base of the potato stem, and the disease phenotype of each treatment was investigated after 30 days. The results showed that compared with the treatment with distilled water, i.e., a concentration of 0 mg / L, the development of Verticillium wilt in the susceptible variety "Désirée" and the moderately resistant variety "Zhongshu 19" to which ABT rooting powder at concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L was applied was significantly delayed, and the death time of the plants was significantly delayed. Among them, the 10 mg / L treatment had the best effect, as shown in the following table. Figure 2 As shown, in the figure, A represents the phenotype of "Désirée" 30 days after inoculation with non-alfalfa Verticillium Vn011; B represents the phenotype of "Zhongshu 19" 30 days after inoculation with non-alfalfa Verticillium Vn011; CK represents healthy potatoes; Vn011+0~40mg / L represents potatoes inoculated with Vn011 and treated with 0~40mg / L concentration of ABT rooting powder; scale bar = 12cm.

[0034] As a specific example, in step 22), 3 days after inoculation with non-alfalfa Verticillium Vn011, i.e., the pathogen, 100 mL of 10 mg / L ABT rooting powder is measured and poured on the base of potato stems. The potato phenotype effect after treatment with 10 mg / L is the best.

[0035] As a specific example, in step 22), the disease grade of potatoes was counted 20 days, 30 days, and 40 days after inoculation with non-alfalfa Verticillium. The results showed that the disease grade of potatoes treated with ABT rooting powder was significantly reduced compared with the control. Under the treatment of 10 mg / L, the number of high-disease grade plants of Désirée and Zhongshu 19 was the lowest at each time point. Figure 3 A and Figure 3 C. The trend of disease index showed that with the extension of inoculation time, the disease index of each treatment of Désirée and Zhongshu 19 gradually increased, and the severity of the disease gradually worsened. Compared with the distilled water treatment with a concentration of 0 mg / L, the disease index of each treatment with a concentration of 5 mg / L to 40 mg / L was significantly lower at each time point, among which the disease index of the treatment with a concentration of 10 mg / L was the lowest. Figure 3 B and Figure 3 D. In summary, ABT rooting powder treatment can delay the development of potato Verticillium wilt and reduce the disease index of Verticillium wilt, among which 10 mg / L concentration of ABT rooting powder has the best effect. In the figure, A and C represent the disease levels of Désirée and Zhongshu 19, respectively; B and D represent the disease index of Désirée and Zhongshu 19, respectively; 20dpi, 30dpi, and 40dpi represent 20 days, 30 days, and 40 days after inoculation with Vn011, respectively; CK represents healthy potatoes; Vn011+0~40mg / L represents potatoes inoculated with Vn011 and treated with 0~40mg / L concentration of ABT rooting powder.

[0036] As a specific example, in step 22), the root systems of various potato varieties inoculated with non-alfalfa Verticillium Vn011 were further analyzed. Compared with the treatment with distilled water, i.e., the ABT rooting powder with a concentration of 0 mg / L, the potato roots treated with the concentration of 5 mg / L to 40 mg / L grew more and better. Specifically, Figure 4As shown, A and B represent the root phenotypes of Désirée and Zhongshu 19 respectively, scale bar = 10 cm; C represents the total root length; D represents the total root volume; E represents the total root surface area; F represents the number of root tips; CK represents healthy potatoes; Vn011+0~40mg / L represents potatoes inoculated with Vn011 and treated with 0~40mg / L concentration of ABT rooting powder. Specifically, the root length, root volume, root surface area and number of root tips of Désirée under the treatments of 5 mg / L, 10 mg / L, 20 mg / L and 40 mg / L concentrations increased by 93.28%-290.03%, 43.06%-336.87%, 78.05%-315.69% and 46.44%-152.22% respectively compared with the treatment of distilled water, i.e., 0 mg / L concentration; the root length, root volume, root surface area and number of root tips of Zhongshu 19 increased by 29.21%-173.94%, 49.78%-345.96%, 232.76%-578.99% and 69.49%-388.54% respectively. Among them, when the concentration was 10 mg / L, the root length, root volume, root surface area, and root tip number of the two materials increased the most. As the concentration of ABT rooting powder was further increased, the increase in each index decreased. Therefore, ABT rooting powder treatment can promote the growth and development of potato roots, delay the disease progression of aboveground potato plants, and enhance potato disease resistance. Among them, the best effect was achieved when the concentration of ABT rooting powder was 10 mg / L.

[0037] As a specific example, in step 22), in order to further clarify the effect of ABT rooting powder on the yield of diseased potatoes, the weight of individual potatoes and the number of individual potatoes of each treatment were counted. The results showed that compared with healthy potatoes (CK), the weight of individual potatoes of Désirée and Zhongshu 19 were significantly reduced after inoculation with pathogens, and the number of individual potatoes did not decrease significantly. Figure 5 As shown in Table 1, A in the figure represents the yield of 16 Désirée plants; B represents the yield of 16 Zhongshu 19 plants; scale = 5 cm; CK represents healthy potatoes; Vn011+0-40 mg / L represents potatoes inoculated with Vn011 and treated with 0-40 mg / L concentration of ABT rooting powder. Among them, the weight of Désirée per plant under the treatment of concentrations of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L was reduced by 74.82%, 62.26%, 34.36%, 55.65%, and 69.60% respectively compared with CK; the weight of Zhongshu 19 per plant was reduced by 65.34%, 61.69%, 31.30%, 48.98%, and 56.51% respectively. The above results show that ABT rooting powder treatment can reduce the yield loss of potato caused by Verticillium wilt. Among them, when the concentration of ABT rooting powder was 10 mg / L, the potato yield loss was the least.

[0038] Table 1 Analysis of potato yield after ABT rooting powder treatment

[0039]

[0040] The comprehensive data of the disease severity of the aboveground part of potatoes, the root system and yield of the underground part show that ABT rooting powder treatment can promote the growth and development of potato roots, delay the progression of Verticillium wilt, delay the death of plants, and reduce the yield loss caused by premature death of plants. Among them, when the concentration of ABT rooting powder is 10mg / L, the control effect of potato Verticillium wilt is the best.

[0041] Compared with the prior art, the method for preventing and controlling potato Verticillium wilt by using ABT rooting powder provided by the present invention comprises the following steps: watering the ABT rooting powder of different concentrations screened out after dilution on the base of potato stems inoculated with non-alfalfa Verticillium wilt Vn011, and comprehensively analyzing the disease severity of the aboveground part of the potato and the data on the root system and yield of the underground part, indicating that ABT rooting powder treatment can promote the growth and development of potato roots, delay the progression of Verticillium wilt, delay the time of plant death, and reduce the yield loss caused by premature plant death. Among them, when the concentration of ABT rooting powder is 5mg / L~40mg / L, it can significantly promote the growth of potato roots and plants; when the treatment concentration of ABT rooting powder is 10mg / L, the disease resistance effect is the best. Indicators such as disease grade, disease index and root growth show that this concentration can significantly enhance the resistance of potato to Verticillium wilt and delay the death of plants; when the treatment concentration of ABT rooting powder is 10mg / L, the yield loss is the lowest. The yield reduction rates of the medium-resistant variety Zhongshu 19 and the susceptible variety Désirée are 31.30% and 34.36% respectively, which significantly reduces the yield loss and prevents and controls potato Verticillium wilt.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for preventing and treating potato Verticillium wilt using ABT rooting powder, characterized in that: include: 1) Screening of ABT rooting powder concentration: 1 g ABT rooting powder was added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to a concentration of 0 mg / L to 80 mg / L, sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings were taken out, the roots were cut off, and the base of the potato stem was soaked in ABT rooting powder with a concentration of 0 mg / L to 80 mg / L for 20 minutes, and then the potato test tube seedlings were planted in a hydroponic box. After 7 days, the growth of the potato root system was observed, and the ABT rooting powder with a concentration of 5 mg / L to 40 mg / L was screened for use; 2) ABT rooting powder to prevent and control potato wilt: 21) Inoculation of potato plants with Verticillium non-lucerne: Two-week-old Désirée and Zhongshu 19 test tube seedlings were planted in pots. When the potatoes grew to 4 to 6 true leaves, i.e. 2 to 3 weeks after planting, a concentration of 1×10 7 80 mL of the non-alfactoid Verticillium medicagogue Vn011 spore suspension with cfu / mL was poured on the base of potato stems, and an equal amount of distilled water was used to inoculate the control plants; 22) ABT rooting powder treatment: 3 days after inoculation with non-alfalfa Verticillium sphaeroides Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., ABT rooting powder with a concentration of 0 mg / L and 5 mg / L-40 mg / L, was respectively measured and poured on the base of the potato stem; 20, 30, and 40 days after inoculation with the pathogen, the disease level was counted and the disease index was calculated; 50 days after inoculation with the pathogen, the stem segments at the base of the potato stem were taken for pathogen isolation, the potatoes were harvested and the yield was measured, and the growth of the potato root system was observed and counted.

2. The method for preventing and treating potato Verticillium wilt by using ABT rooting powder according to claim 1, characterized in that: The ABT rooting powder added in step 1) was purchased from Beijing ABT Biotechnology Co., Ltd.

3. The method for preventing and treating potato Verticillium wilt by using ABT rooting powder according to claim 1, characterized in that: In the step 1), 1 g of ABT rooting powder is added to 100 mL of 75% alcohol to fully dissolve, and then diluted with distilled water to concentrations of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L, respectively, and sealed with tin foil and placed in a cool place for use; 2-week-old Désirée and Zhongshu 19 test tube seedlings are taken out, the roots are cut off, and the base of the potato stem is soaked in distilled water with a concentration of 0 mg / L and a concentration of 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 60 mg / L, and 80 mg / L ABT rooting powder for 20 minutes, and then the potato test tube seedlings are planted in a hydroponic box, and the growth of the potato root system is observed after 7 days, and finally the ABT rooting powder with a concentration of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L is selected for use; In the step 22), 3 days after the inoculation of non-alfalfa Verticillium Vn011, i.e., the pathogen, 100 mL of distilled water, i.e., ABT rooting powder with a concentration of 0 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L, was respectively measured and poured on the base of the potato stem.

4. The method for preventing and treating potato Verticillium wilt by using ABT rooting powder according to claim 3, characterized in that: In the step 22), 3 days after the inoculation of the pathogenic bacteria, 100 mL of 10 mg / L ABT rooting powder was measured and poured on the base of the potato stem.

5. The method for preventing and treating potato Verticillium wilt by using ABT rooting powder according to claim 3, characterized in that: In the step 22), compared with the treatment with distilled water (i.e., the treatment with a concentration of 0 mg / L), the root length, root volume, root surface area, and root tip number of Desirée under the treatment with concentrations of 5 mg / L, 10 mg / L, 20 mg / L, and 40 mg / L increased by 93.28% to 290.03%, 43.06% to 336.87%, 78.05% to 315.69%, and 46.44% to 152.22%, respectively; the root length, root volume, root surface area, and root tip number of Zhongshu 19 increased by 29.21% to 173.94%, 49.78% to 345.96%, 232.76% to 578.99%, and 69.49% to 388.54%, respectively.

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