Preparation method of ratio fluorescent chromatographic test strip and application in detection of patulin

By preparing a ratiometric fluorescent probe combining blue carbon dots and thiol-functionalized gold nanoclusters, and preparing a ratiometric fluorescence chromatography test strip, the sensitivity and portability problems of patulin detection were solved, and rapid and stable visual detection was achieved.

CN119438161BActive Publication Date: 2025-10-10HEFEI INSTITUTE OF PHYSICAL SCIENCE CHINESE ACADEMY OF SCIENCES
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Patent Information

Application Number
CN202411680045.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-22
Publication Date
2025-10-10
Estimated Expiration
2044-11-22

AI Technical Summary

Technical Problem

The existing technology for detecting patulin has problems such as sensitivity being easily affected by the environment, antigens and antibodies being difficult to obtain, and the detection device being insufficiently portable, resulting in insufficient detection accuracy and convenience.

Method used

A ratiometric fluorescent probe preparation method was adopted to combine blue carbon dots with thiol-functionalized gold nanoclusters to form a fluorescent system with red fluorescence quenching and blue fluorescence activation. Ratiometric fluorescence chromatography test strips were prepared for the visual detection of patulin.

Benefits of technology

Rapid and stable patulin detection was achieved with a response time of less than 10 seconds and a detection limit of 0.019 μM. It has good selectivity and anti-interference properties and is suitable for convenient carrying.

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Abstract

The application discloses a preparation method of a ratio fluorescent chromatography test strip and application in detection of patulin, and comprises the following steps: S1, preparation of blue carbon dots; S2, preparation of gold nanoclusters; S3, preparation of a ratio fluorescent probe. The application is based on a strategy of red fluorescence quenching and blue fluorescence opening. The solution of thiol functionalized AuNCs is rich in functional groups (-SH) and is combined with patulin. Red fluorescence is gradually quenched, and blue fluorescence is opened, so that the function of detecting patulin is realized. The change of fluorescence color is from red to blue, and the whole fluorescence signal response can be completed within 10 seconds. In addition, with the continuous increase of the concentration of patulin, the red fluorescence is gradually quenched, the blue fluorescence is gradually enhanced, and a series of changes in fluorescence intensity is presented under an ultraviolet lamp, so that the visual detection of patulin is realized. The application can realize convenient and rapid preliminary detection of food health.
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