Nutrient solution for improving survival rate of guava seedlings and preparation method thereof

Abstract

The invention relates to the field of plant cultivation, in particular to a nutrient solution for increasing the survival rate of guava seedlings and a preparation method of the nutrient solution. According to the composition for improving the survival rate of guava seedling culture, the seaweed oligosaccharide in the composition can continuously provide nutrients for guavas, improve photosynthesis of the guavas, prevent adverse effects of severe environments and heavy metals in soil on the guavas, improve stress resistance and disease resistance of the guavas and improve the survival rate of guava seedlings. The thickening of vascular bundles in the root hair region of the guava is promoted, and the expression of genes synthesized with indole substances is enhanced; the plant hormone simulates the biological action of plant endogenous auxin and can promote the growth and development of the root of the guava; by combining the three components of leonuride, naringenin and resveratrol, the oxidation resistance of the root system of the guava can be further enhanced, the oxidative stress of the root system is reduced, the nutrient absorption capacity of the root system is improved, the aim of improving the survival rate of the guava seedling culture is finally achieved, and the survival rate reaches 99% or above.

Description

Technical Field

[0001] The invention relates to the field of plant cultivation, and in particular to a nutrient solution for improving the survival rate of guava seedlings and a preparation method thereof. Background Art

[0002] Guava (scientific name: Psidium guajava Linn.), also known as Hua Nian, Guava, Chicken Shit Fruit, Trumpet Guava, belongs to the Myrtaceae family. It is up to 13 meters tall. The bark is smooth, gray, and flaky. The young branches are ridged and hairy. The leaves are leathery, oblong to elliptical, with an acute or blunt tip, a nearly round base, slightly rough on the upper side, and hairy on the lower side. The lateral veins are 12-15 pairs, often sunken, and the reticulate veins are obvious. The petiole is 5mm long. The flowers are solitary or 2-3 flowers are arranged in cymes. The calyx tube is bell-shaped and hairy, and the calyx cap is irregularly split. The petals are white. The stamens are 6-9mm long. The ovary is inferior and fused with the calyx, and the style is the same length as the stamens. The berries are spherical, oval or pear-shaped, with persistent sepals at the top, the flesh is white and yellow, the placenta is hypertrophic, fleshy, and light red, and the number of seeds is large. Guava fruit is light green, fragrant and delicious, rich in nutrients, containing protein, fat, sugar, vitamins and trace elements such as phosphorus, calcium and magnesium. Eating it regularly can not only supplement the nutrients that the human body lacks, but also strengthen the body and improve physical fitness. In addition to being eaten fresh, guava fruit can also be made into jam, jelly and juice, etc. It can also be used as medicine. It has the functions of astringent, antidiarrheal, hemostatic and antipruritic. It is mainly used to treat diarrhea, chronic dysentery, eczema, traumatic bleeding and other diseases. It also has a good effect on improving the symptoms of diabetes.

[0003] The propagation methods of guava seedlings are: sowing, layering, cuttings and grafting. The sowing seedling raising cycle is long, and the offspring are likely to have a high probability of trait separation, which is not conducive to the inheritance of the excellent traits of the parents. Except for the selection of new varieties and the cultivation of grafted rootstocks, sowing seedlings is generally not used. Layering, cuttings and grafting seedlings can all stably inherit the excellent traits of the parents. Grafting seedlings are affected by many factors such as the affinity of different varieties of rootstocks and scions, grafting technology and climatic conditions. The grafting survival rate is unstable and the seedling raising cycle is long, resulting in high costs for grafting seedling cultivation. Both layering and cutting seedlings belong to asexual reproduction, which can maintain the traits of the parents and is conducive to the stable inheritance of excellent traits. Layering seedlings have low branch utilization, inconvenient operation, and it is difficult to achieve large-scale production. Cutting seedlings have the advantages of high branch utilization, convenient operation, short seedling raising cycle, and large-scale production. It is the best method for guava seedling cultivation. At present, the main factors restricting guava seedling cultivation are slow growth of seedlings and difficulty in rooting or germination of cutting branches, resulting in a low survival rate of cuttings. For example, Guo Zhenyue et al. (CN112120041A) successfully cultivated 'Pearl' guava cuttings using a rooting agent (NAA 300-340mg / L+IBA 100-140mg / L+ammonia 8-12mL / L), and the survival rate was 60.77%-66.57%. Therefore, finding a nutrient solution that can improve the survival rate of guava seedlings has a significant impact on guava breeding. Summary of the invention

[0004] The purpose of the present invention is to provide a nutrient solution for improving the survival rate of guava seedlings and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art. The composition and nutrient solution provided by the present invention can effectively improve the survival rate of guava to more than 99%.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a composition for improving the survival rate of guava seedlings, the composition comprising leonurin, algae oligosaccharide, naringenin, resveratrol and plant hormones;

[0007] The mass ratio of the leonurin, the algae oligosaccharide, the naringenin, the resveratrol and the plant hormone is (23-54): (20-40): (10-15): (10-12): (28-36).

[0008] Preferably, the mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 35:27:10:10:30;

[0009] Or, the mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 28:35:13:11:35;

[0010] Or, the mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 50:20:15:12:28.

[0011] Preferably, the plant hormone is indoleacetic acid.

[0012] The present invention provides application of the above-mentioned composition in preparing a nutrient solution for improving the survival rate of guava seedlings.

[0013] The invention provides a nutrient solution for improving the survival rate of guava seedlings, and the nutrient solution comprises the above-mentioned composition.

[0014] Further preferably, in terms of concentration, the nutrient solution comprises components of the following concentrations:

[0015] 23-54mg / L Leonurin, 20-40mg / L Algae Oligosaccharides, 10-15mg / L Naringenin, 10-12mg / L Resveratrol and 28-36mg / L Plant Hormones.

[0016] Further preferably, in terms of concentration, the nutrient solution comprises components of the following concentrations:

[0017] 35mg / L Leonurin, 27mg / L Algae Oligosaccharides, 10mg / L Naringin, 10mg / L Resveratrol and 30mg / L Plant Hormones;

[0018] and / or, 28mg / L leonurin, 35mg / L seaweed oligosaccharides, 13mg / L naringenin, 11mg / L resveratrol and 35mg / L plant hormones;

[0019] And / or, 50 mg / L of leonurin, 20 mg / L of seaweed oligosaccharides, 15 mg / L of naringenin, 12 mg / L of resveratrol and 28 mg / L of plant hormones.

[0020] Preferably, the nutrient solution also includes a solvent.

[0021] Preferably, the solvent is dimethyl sulfoxide.

[0022] The present invention provides a method for preparing the above-mentioned nutrient solution, comprising the step of mixing the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone to obtain the nutrient solution.

[0023] The present invention provides a method for preparing the above-mentioned nutrient solution, comprising the step of mixing the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol, the plant hormone and the solvent to obtain the nutrient solution.

[0024] The present invention provides the use of the above-mentioned composition or the above-mentioned nutrient solution in improving the survival rate of guava seedlings

[0025] Further preferably, the present invention also provides a method for improving the survival rate of guava seedlings, comprising the step of dipping the roots of guava cuttings in a diluted solution of the above-mentioned nutrient solution.

[0026] Further preferably, the dilution factor of the nutrient solution is 800-1000 times.

[0027] Further preferably, the root dipping treatment lasts for 20-25 seconds.

[0028] Further preferably, the guava cuttings are one-year-old guava shoots.

[0029] Further preferably, the length of the guava cuttings is 10-15 cm.

[0030] The present invention discloses the following technical effects:

[0031] The present invention provides a composition for improving the survival rate of guava seedlings, comprising motherwort glycoside, seaweed oligosaccharide, naringenin, resveratrol and plant hormones. The seaweed oligosaccharide in the composition of the present invention can continuously provide nutrients for guava, improve the photosynthesis of guava, enable it to produce enough products for its own growth, and prevent the adverse effects of harsh environment and heavy metals in the soil on guava, improve the stress resistance and disease resistance of guava, promote the thickening of vascular bundles in the root hair area of ​​guava, and enhance the gene expression of indole-type substance synthesis; the plant hormone imitates the biological effect of plant endogenous auxin, and can promote the growth and development of guava roots; combined with the three components of motherwort glycoside, naringenin and resveratrol, the antioxidant capacity of the guava root system can be further enhanced, the root system oxidative stress can be reduced, and the root system's ability to absorb nutrients can be improved, and finally the purpose of improving the survival rate of guava seedlings can be achieved, and the survival rate is more than 99%. DETAILED DESCRIPTION

[0032] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0033] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0034] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0035] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0036] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0037] The chemical formula of Leonurus glycoside is C 15 H 24 O9, molecular weight is 348.35, CAS registration number is 52949-83-4, and its structural formula is shown in Formula I:

[0038]

[0039] In a specific embodiment of the present invention, the leonurin is commercially available; if it is prepared by itself, the following method can be used:

[0040] Step 1: Dry, crush and sieve the Chinese medicinal material Rehmannia glutinosa;

[0041] Step 2: Add anhydrous ethanol to the material obtained in the previous step and reflux extract twice, each reflux extraction time is 2 hours, combine the extracts, recover the solvent and concentrate to an appropriate amount to obtain a concentrated solution;

[0042] Step 3: The concentrated solution obtained in the previous step is placed in a device with a macroporous resin for adsorption treatment to obtain a crystal;

[0043] Step 4: adding ethyl acetate to the crystals obtained in the previous step to dissolve, and eluting with anhydrous ethanol to obtain liquid crystals;

[0044] Step 5: Crystallize and purify the liquid obtained in the previous step.

[0045] Algae oligosaccharides (AOS) are small molecular fragments formed by enzymatic degradation of algae polysaccharides (alginic acid). Algae oligosaccharides have good water solubility and small molecular weight, and are easier to be absorbed and utilized by plants than algae polysaccharides. In addition, algae oligosaccharides also have many biological activities that algae polysaccharides do not have.

[0046] In a specific embodiment of the present invention, the seaweed oligosaccharide is commercially available; if it is prepared by itself, the following method can be used:

[0047] Step 1: Mix the seaweed with 3 times the amount of water, soak it, then add 2% sodium chloride solution with a mass fraction of 10% of the total mass of the seaweed, stir evenly, and then wash it repeatedly with water for 2 times;

[0048] Step 2: adding chitosan in an amount of 10% of the total mass of the product of step 1 to the product of step 1 to adjust the montmorillonite, and then ball milling in a ball mill for 10 minutes at a ball milling speed of 1000 r / min, and then completing the ball milling to obtain a ball milled material;

[0049] Step 3: Add the ball mill to the enzymatic hydrolysis tank, and then add 10% of the total mass of the ball mill to perform enzymatic hydrolysis. The temperature of the enzymatic hydrolysis is 26°C and the enzymatic hydrolysis is performed for 2 hours.

[0050] Step 4: Use ultrafiltration to separate the enzymatic hydrolysis product, collect the separated liquid, and perform vacuum concentration and microwave drying to obtain seaweed oligosaccharides; the membrane pore size of ultrafiltration separation is 0.6nm, and the pressure is 0.5MPa; the pressure of vacuum concentration is 85KPa, the temperature is 45°C, and the time is 1h; the temperature of microwave drying is 65°C, the pressure is 85KPa, and the time is 2h.

[0051] The preparation method of chitosan regulating montmorillonite agent is:

[0052] S01: Add montmorillonite to 3 times the mass fraction of 10% sodium alginate aqueous solution and stir evenly, then add 10% of the total mass of montmorillonite silane coupling agent KH560 and 2% of the total mass of montmorillonite glycolic acid, stir well, and obtain montmorillonite compounding solution;

[0053] S02: adding nano-silicon dioxide to a 2% hydrochloric acid solution at a weight ratio of 1:5, then adding 2% dodecyltrimethylammonium bromide of the total weight of the nano-silicon dioxide, stirring evenly, then washing with water and drying to obtain modified nano-silicon dioxide;

[0054] S03: adding modified nano-silicon dioxide to the montmorillonite compounding liquid in a weight ratio of 1:5, stirring and reacting, washing with water and drying to obtain a nano-silicon dioxide modified montmorillonite compounding agent; the stirring reaction is carried out at a speed of 450 r / min, a stirring temperature of 40° C., and a stirring time of 30 min;

[0055] S04: The nano-silica modified montmorillonite compounding agent is mixed with 2 times the volume of chitosan aqueous solution, stirred and dispersed evenly to obtain a chitosan adjusted montmorillonite agent; the mass fraction of the chitosan aqueous solution is 6%.

[0056] The preparation method of the enzymatic modifier is as follows:

[0057] S11: placing hydroxyapatite in a sulfuric acid aqueous solution with a volume 5 times that of hydroxyapatite and stirring evenly, then washing with water and drying; the mass fraction of the sulfuric acid aqueous solution is 2%;

[0058] S12: washing and peeling the yam, collecting the yam mucus, taking 10 parts of the yam mucus, and then adding 2 parts of aloe extract to the yam mucus, and stirring evenly; the preparation method of the aloe extract is: sending the aloe into a crusher to crush, and then adding 2 times the volume of water to boil for 1 hour, and then turning to a low heat to continue processing for 35 minutes, and the processing is completed to obtain the aloe extract;

[0059] S13: Add 5 parts of Bacillus to S12, then add 1 part of the product of S11, stir evenly, and obtain an enzymatic modifier.

[0060] Naringenin forms a glycoside with neohesperidin at the 7th carbon position, called naringin, which tastes extremely bitter. When it is ring-opened and hydrogenated under alkaline conditions to form dihydrochalcone compounds, it is a sweetener with a sweetness that can be 2000 times that of sucrose. Naringenin is the aglycone of naringin, a dihydroflavonoid compound, which has antibacterial, anti-inflammatory, free radical scavenging, antioxidant, cough and expectorant, lipid-lowering, anti-cancer and anti-tumor, spasmolytic and choleretic, prevention and treatment of liver disease, inhibition of platelet aggregation, and anti-atherosclerosis. It can be widely used in medicine, food and other fields.

[0061] In a specific embodiment of the present invention, the naringenin is commercially available; if it is prepared by itself, the following method can be used:

[0062] (1) 20 g of naringin-containing fruit was mixed with a 4.5 W / V% hydrochloric acid solution at a solid-liquid ratio of 1 g: 100 mL, and the mixture was stirred for hydrolysis at 95°C for 1 h, then rapidly cooled to room temperature and allowed to stand for 12 h to precipitate a solid. After suction filtration, the solid was washed with purified water until neutral, and then the crude naringin was obtained;

[0063] (2) The crude naringenin obtained in step (1) was added to an acetic acid solution with a concentration of 30 W / V% at a solid-liquid ratio of 1 g: 7 mL, stirred at room temperature for 30 min, and then the solid was added to the above-mentioned acetic acid solution of equal concentration and stirred for 15 min after filtration. The solid was then washed with water until neutral, and then dissolved in 100 mL of 95% ethanol by volume at a solid-liquid ratio of 1 g: 10 mL. The solid was then passed through a neutral alumina chromatography column, and the chromatography column was washed with 40 mL of 95% ethanol by volume. The solutions after passing through the chromatography column were combined, and 14 g of activated carbon was added to the solution. The solid was then heated to room temperature. The mixture was stirred for 30 min at room temperature. After filtration, 6 g of activated carbon was added to the filtrate, and the mixture was stirred for 30 min at room temperature. After filtration, the filtrate was heated and concentrated to 1 / 3 of the volume. An equal volume of distilled water was added while the mixture was hot. After cooling to room temperature, the mixture was placed in a refrigerator and allowed to stand at 5°C for 24 h for crystallization. After the white needle-shaped crystals were completely precipitated, the wet naringenin product was obtained by filtration. The wet naringenin product was dissolved by heating with 95% ethanol at a solid-liquid ratio of 1 g:6 mL. The distilled water equal to the volume of the ethanol was added for recrystallization. The precipitated white needle-shaped crystals were collected by filtration and then dried to obtain the product.

[0064] Resveratrol is a polyphenol compound, also known as stilbene triphenol, mainly derived from plants such as peanuts, grapes, knotweed, and mulberry. Chinese aliases of resveratrol: 3,4',5-trihydroxystilbene, knotweed aglycone, stilbene triphenol, stilbene triphenol, 3,4',5-trihydroxystilbene, 3,4',5-trihydroxystilbene, (E)-5-[2-(4-hydroxyphenyl)-vinyl]-1,3-benzenediol, 3,4,5-trihydroxy trans (E)-3,5,4'-trihydroxystilbene; its CAS number is 501-36-0. Resveratrol is a natural polyphenol with strong biological properties. It is a chemopreventive agent for tumors, and is also a chemopreventive agent for reducing platelet aggregation, preventing and treating atherosclerosis, and cardiovascular and cerebrovascular diseases.

[0065] In a specific embodiment of the present invention, the resveratrol is commercially available; if it is prepared by itself, the following method can be used:

[0066] The method is prepared by using the preparation process of 3,5-dimethoxybenzyl chloride, the preparation process of 3,5-dimethoxybenzyl phosphonic acid diethyl ester, the preparation process of (E)-3,4',5-trimethoxystilbene and the preparation process of resveratrol; specifically:

[0067] Ⅰ. Preparation process of 3,5-dimethoxybenzyl chloride:

[0068] 33.6 kg of 3,5-dimethoxybenzyl alcohol and 50.4 kg of Lucas reagent were thrown into a reactor, stirred at room temperature, and maintained for 1 hour to obtain a mixed solution I; the mixed solution I was filtered to obtain a white solid; then the filtered white solid was washed three times with 20 L of distilled water; finally, the washed white solid was dried by vacuum drying to obtain 3,5-dimethoxybenzyl chloride;

[0069] II. Preparation process of diethyl 3,5-dimethoxybenzylphosphonate:

[0070] First, 37.3 kg of the prepared 3,5-dimethoxybenzyl chloride and 74.6 kg of triethyl phosphite are thrown into a reactor for heating and stirring reaction. The temperature in the reactor is controlled at 90° C., and reflux condensation is performed with a cooler and maintained for 3 hours. The material obtained by reflux condensation is then recovered under 100° C. and 20 mmHg vacuum conditions to obtain triethyl phosphite. The remaining liquid in the reactor is diethyl 3,5-dimethoxybenzylphosphonate.

[0071] III. Preparation process of (E)-3,4',5-trimethoxyphenylethylene:

[0072] First, 43.2 kg of the prepared diethyl 3,5-dimethoxybenzylphosphonate and 86.4 kg of dry N,N-dimethylformamide are put into a reactor and stirred to obtain a mixed solution II; then, p-methoxybenzaldehyde is dissolved in dry DMF to prepare a dry DMF solution of p-methoxybenzaldehyde; then, the reactor containing the mixed solution II is cooled with an ice bath, and when the mixed solution II is cooled to below 0°C, sodium methoxide is added under rapid stirring, and the added sodium methoxide is stirred to be uniformly dispersed. After the sodium methoxide is added, the dry DMF solution of p-methoxybenzaldehyde is slowly added dropwise to obtain a mixed solution III; then, the mixed solution III is placed in a reactor, the temperature is naturally raised to room temperature, and the reaction is performed for 10 hours to obtain a mixed solution IV;

[0073] Then, the obtained mixed solution IV is poured into ice water, stirred, and diluted hydrochloric acid (volume concentration is 10%) is added dropwise for neutralization. Solid is precipitated during the neutralization process, and a solid-liquid mixture is obtained after the reaction is complete. Finally, the obtained solid-liquid mixture is filtered to obtain a light yellow solid. The light yellow solid is recrystallized with an ethanol / water (40%) mixed solvent to obtain (E)-3,4',5-trimethoxystilbene.

[0074] IV. Preparation process of resveratrol:

[0075] First, add the prepared 3kg (E)-3,4',5-trimethoxystilbene and 20L anhydrous pyridine into the reactor and stir; install a reflux condenser on the reactor, heat the reactor in an oil bath to slowly increase the temperature of the reactor, and add anhydrous aluminum chloride into the reactor in batches when the temperature is controlled at 110±2°C;

[0076] Next, after adding anhydrous aluminum chloride, the temperature of the reactor is raised to 155±2°C, and the mixture V is reacted at a constant temperature for about 4 hours to obtain the mixture V; after the reaction is completed, the mixture V is poured into ice water while hot to be hydrolyzed to obtain a hydrolysis product, and white smoke is generated;

[0077] Finally, the obtained hydrolyzate was extracted with ethyl acetate at 40°C, and then the liquids were separated and the organic phase was cooled and crystallized to finally obtain resveratrol crystals.

[0078] Plant hormones, also known as natural plant hormones or endogenous plant hormones, refer to some trace organic compounds produced in plants that can regulate (promote or inhibit) their own physiological processes. There are six major types of hormones known to be produced in plants, namely auxins, gibberellins, cytokinins, abscisic acid, ethylene and brassinosteroids. They are all simple small molecule organic compounds, but their physiological effects are very complex and diverse. From affecting cell division, elongation, differentiation to affecting plant germination, rooting, flowering, fruiting, sex determination, dormancy and shedding. In the present invention, plant hormones specifically refer to indoleacetic acid. The Chinese aliases for indoleacetic acid are 3-indoleacetic acid, indole-3-acetic acid, indole-3-acetic acid (IUPAC), 3-indoleacetic acid, β-indoleacetic acid, 2-(3-indolyl)acetic acid; its chemical formula is C 10 H9NO2, molecular weight is 175.18, CAS registration number is 87-51-4.

[0079] In a specific embodiment of the present invention, the indoleacetic acid is commercially available; if it is prepared by itself, the following method can be used:

[0080] Take 10kg of dried kelp, extract it with anhydrous ethanol 20 times the weight of kelp for 10 days, rotary evaporate the extract, set the water bath temperature to 55℃, evaporate to dryness to obtain extract, recover anhydrous ethanol, dissolve the extract with 30% ethanol, filter through filter paper to remove insoluble matter, and chromatograph the filtrate on a macroporous resin column. The pretreatment procedure of the resin is as follows: take 1000mL of macroporous resin AB-8 and place it in a 3000mL triangular flask. Pretreatment is first soaked in 30% ethanol for 20h to allow the resin to absorb water and fully develop the resin. After that, replace the ethanol solution, place the fully developed resin on a shaker for 2h, and the shaker frequency is 200r / min. Wash the resin repeatedly with clean water until the water is clear, without turbidity and impurities. Wash the resin with ultrapure water, shake it on a shaker for 2h, and replace the ultrapure water. Place the flask on a shaker, soak it in 2 times the volume of 2% NaOH and shake it on the shaker for 2 hours, then wash it with pure water until it is neutral; then shake it on the shaker for 2 hours with 2 times the volume of 2% HCl solution, absorb the solution, and then add 4% HCl solution to just immerse the resin for later use. Put the treated resin into a beaker, add a small amount of water to stir, and slowly pour the resin into the chromatography column along the wall. Elute with 70% ethanol at a flow rate of 2mL / min, and collect 3000mL of eluent. Concentrate to dryness, recover ethanol, and obtain natural indoleacetic acid.

[0081] Example 1 Preparation of nutrient solution for improving the survival rate of guava seedlings

[0082] The invention discloses a nutrient solution for improving the survival rate of guava seedlings by mixing leonurin, algal oligosaccharide, naringenin, resveratrol, indoleacetic acid and dimethyl sulfoxide. The nutrient solution comprises leonurin in a concentration of 23-54 mg / L, sodium alginate in a concentration of 20-40 mg / L, naringenin in a concentration of 10-15 mg / L, resveratrol in a concentration of 10-12 mg / L and indoleacetic acid in a concentration of 28-36 mg / L.

[0083] Example 2 Screening of Material Combinations

[0084] 1. Test materials:

[0085] New shoots of one-year-old 'Pearl' guava.

[0086] 2. Experimental design:

[0087] The different groups are detailed in Table 1, and the solvent is dimethyl sulfoxide.

[0088] Table 1 Design of nutrient solution for improving the survival rate of guava seedlings

[0089]

[0090]

[0091] 3. Test steps:

[0092] (1) Substrate preparation: loess soil and vermiculite were prepared in a volume ratio of 3:2. One day before cutting, the soil was disinfected by soaking it with 0.5% potassium permanganate and then placed on a tray (seedling tray specifications: 60 cm × 30 cm × 7 cm, 15 holes / tray) for later use.

[0093] (2) Preparation of cuttings: Cut a 1-year-old 'Pearl' guava shoot as a cutting, about 10-15 cm in length, cut the top of the cutting flat, cut the base at 30-45°, cut off the two pairs of leaves at the base, and keep the top pair of leaves. Soak the cuttings completely in 70% 1000 times thiophanate-methyl solution for 5 minutes, take out the cuttings and rinse them with distilled water, dry them in the shade for later use.

[0094] (3) Dilute each nutrient solution in Table 1 800 times to obtain each treatment dilution solution.

[0095] (4) Cuttings: Before cuttings, use chopsticks to make holes in the plug tray matrix prepared in step (1), dip the base of the cutting branch (about 5 cm long) in the dilution of each treatment in step (3) for 20 seconds, insert the cutting branch into the small hole of the plug tray matrix, gently compact the matrix, and then water it thoroughly. Each treatment was repeated 3 times, with 100 cutting branches in each repeat.

[0096] (5) Management after cutting:

[0097] 1) After cutting, place the seedling tray in a temperature-controlled greenhouse, and build a small arch shed above the seedling tray and cover it with plastic film to keep it moist;

[0098] 2) Within 14 days after cutting, the humidity in the small shed is maintained above 90% and the temperature is controlled within the range of 23℃-30℃;

[0099] 3) 15-49 days after cutting, open the plastic film at both ends of the small shed for ventilation, maintain the humidity in the small shed at around 80%, and control the temperature within the range of 22℃-35℃; spray water once every 7-10 days to maintain the moisture content of the substrate at around 70%; spray fungicide once every 14 days (alternately use 1000-fold diluted solution of 70% thiophanate-methyl and 1000-fold diluted solution of 75% thiophanate-methyl).

[0100] (6) 50 days after cutting, the survival rate and rooting rate of the cuttings were calculated; the calculation formula for the survival rate was: survival rate = number of surviving branches / total number of cuttings × 100%; the calculation formula for the rooting rate was: rooting rate = number of rooting branches / total number of cuttings × 100.

[0101] 4. Survey Results:

[0102] The survey results are shown in Table 2.

[0103] Table 2 Statistical results of survival rate and rooting rate of guava cuttings

[0104]

[0105]

[0106] As shown in Table 1, only when the five components of motherwort glycoside, seaweed oligosaccharide, naringenin, resveratrol and indoleacetic acid are used in combination, can the survival rate and rooting rate of guava cuttings be significantly improved. When one, two, three or four of them are used in combination, it is found that compared with distilled water, although the survival rate and rooting rate of guava cuttings are improved, the effect is significantly lower than the combination of motherwort glycoside, seaweed oligosaccharide, naringenin, resveratrol and indoleacetic acid; and the effect of indoleacetic acid cannot be replaced by any plant hormone. It can be seen that when motherwort glycoside, seaweed oligosaccharide, naringenin, resveratrol and indoleacetic acid are combined, they have a synergistic effect.

[0107] Example 3 Screening of concentrations of each component

[0108] 1. Test materials:

[0109] New shoots of one-year-old 'Pearl' guava.

[0110] 2. Experimental design:

[0111] The different groups are detailed in Table 3, and the solvent is dimethyl sulfoxide.

[0112] Table 3 Design of nutrient solution for improving the survival rate of guava seedlings

[0113] deal with Components and concentrations 1 35mg / L Leonurin + 27mg / L Algae Oligosaccharide + 10mg / L Naringin + 10mg / L Resveratrol + 30mg / L Indoleacetic Acid 2 28mg / L Leonurin + 35mg / L Algae Oligosaccharide + 13mg / L Naringin + 11mg / L Resveratrol + 35mg / L Indoleacetic Acid 3 50mg / L Leonurin + 20mg / L Algae Oligosaccharide + 15mg / L Naringin + 12mg / L Resveratrol + 28mg / L Indoleacetic Acid 4 20mg / L Leonurin + 20mg / L Algae Oligosaccharide + 8mg / L Naringin + 12mg / L Resveratrol + 50mg / L Indoleacetic Acid 5 18mg / L Leonurin + 45mg / L Algae Oligosaccharide + 15mg / L Naringin + 15mg / L Resveratrol + 25mg / L Indoleacetic Acid 6 60mg / L Leonurin + 25mg / L Algae Oligosaccharide + 8mg / L Naringin + 9mg / L Resveratrol + 40mg / L Indoleacetic Acid 7 57mg / L Leonurin + 48mg / L Algae Oligosaccharide + 20mg / L Naringin + 17mg / L Resveratrol + 25mg / L Indoleacetic Acid 8 Distilled water

[0114] 3. Test steps:

[0115] Same as Example 2.

[0116] 4. Survey Results:

[0117] The survey results are shown in Table 4.

[0118] Table 4 Statistical results of survival rate and rooting rate of guava cuttings

[0119]

[0120]

[0121] In order to explore whether the concentration of each component has an effect on the survival rate and rooting rate of guava cuttings, the concentration of each substance was optimized in this example, and the results of the survival rate and rooting rate of guava cuttings are shown in Table 4. The results show that the concentration of each component has a great influence on the survival rate and rooting rate of guava cuttings, and only the guava cuttings prepared in treatments 1 to 3 have the best survival rate and rooting rate.

[0122] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A composition for improving the survival rate of guava seedlings, characterized in that: The composition comprises leonurin, algal oligosaccharide, naringenin, resveratrol and plant hormones; The mass ratio of the leonurin, the algae oligosaccharide, the naringenin, the resveratrol and the plant hormone is (23-54): (20-40): (10-15): (10-12): (28-36).

2. The composition according to claim 1, characterized in that The mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 35:27:10:10:30; Or, the mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 28:35:13:11:35; Or, the mass ratio of the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone is 50:20:15:12:

28.

3. The composition according to claim 1 or 2, characterized in that The plant hormone is indoleacetic acid.

4. Use of the composition according to any one of claims 1 to 3 in preparing a nutrient solution for improving the survival rate of guava seedlings.

5. A nutrient solution for improving the survival rate of guava seedlings, characterized in that: The nutrient solution comprises the composition according to any one of claims 1 to 3.

6. The nutrient solution according to claim 5, characterized in that The nutrient solution also includes a solvent.

7. The nutrient solution according to claim 6, characterized in that The solvent is dimethyl sulfoxide.

8. The method for preparing the nutrient solution according to claim 5, characterized in that: The method comprises the steps of mixing the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol and the plant hormone to obtain the nutrient solution.

9. The method for preparing the nutrient solution according to claim 6, characterized in that: The method comprises the steps of mixing the leonurin, the seaweed oligosaccharide, the naringenin, the resveratrol, the plant hormone and the solvent to obtain the nutrient solution.

10. Use of the composition according to any one of claims 1 to 3 or the nutrient solution according to any one of claims 5 to 7 in improving the survival rate of guava seedlings.

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