No-wash hand sanitizer and preparation method thereof

Abstract

The invention relates to the technical field of hand sanitizers, in particular to a no-wash hand sanitizer and a preparation method thereof. The no-wash hand sanitizer is prepared from the following components in percentage by mass: 0.5 to 1 percent of modified soybean peptide, 0.8 to 1.2 percent of artemisia japonica polysaccharide, 1 to 3 percent of glycerol, 0.3 to 0.5 percent of carbomer, 0.05 to 0.15 percent of triethanolamine, 0.1 to 0.5 percent of vitamin E, 0.02 to 0.05 percent of rose essence and the balance of distilled water, the structural formula of the modified soybean peptide is as follows: # imgabs0. The no-wash hand sanitizer provided by the invention is excellent in sterilization effect, long in action time, mild, skin-friendly and non-irritant.

Description

Technical Field

[0001] The invention relates to the technical field of hand sanitizer, and in particular to a wash-free hand sanitizer and a preparation method thereof. Background Art

[0002] As people pay more and more attention to health and environmental awareness, hand sanitizers are becoming more and more popular. As a hand cleaning and disinfection product, hand sanitizers can achieve the effect of cleaning and disinfection without using water. They are very convenient to use and are widely used in hospitals, banks, supermarkets, and travel scenes.

[0003] At present, the common hand sanitizers on the market usually use ethanol, cationic surfactants or triclosan as antibacterial ingredients, and add moisturizing ingredients such as glycerin. This type of hand sanitizer has the advantages of good antibacterial effect and low cost. However, ethanol evaporates quickly, which not only accelerates skin moisture loss and irritates the skin, but also makes it difficult to have a long-lasting antibacterial effect. Therefore, it is particularly important to develop a green and safe alcohol-free hand sanitizer with good antibacterial effect. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide a disposable hand sanitizer.

[0005] A second object of the present invention is to provide a method for preparing a disposable hand sanitizer.

[0006] One of the purposes of the present invention is achieved by the following technical solution: A wash-free hand sanitizer, composed of the following ingredients by mass percentage: 0.5-1% modified soybean peptide, 0.8-1.2% artemisia polysaccharide, 1-3% glycerol, 0.3-0.5% carbomer, 0.05-0.15% triethanolamine, 0.1-0.5% vitamin E, 0.02-0.05% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is as follows: .

[0007] Furthermore, the preparation method of the modified soybean peptide is as follows: (1) dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to heat for reaction, adding anhydrous ethanol to precipitate, and centrifuging to obtain compound A; (2) Compound A is added into water and stirred evenly, and 4-formylphenylboronic acid is added to react. After the reaction is completed, the reaction solution is transferred into a dialysis bag, and after dialysis, freeze-dried to obtain a modified soybean peptide.

[0008] Furthermore, in step (1), the ratio of the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide is 5 g: 1-2 mL: 0.5-1.5 mL: 2-3 g; and the concentration of the soybean peptide solution is 0.01 g / mL.

[0009] Furthermore, in step (1), the heating reaction temperature is 45-55°C and the time is 22-26h.

[0010] Furthermore, in step (2), the mass ratio of the compound A to 4-formylphenylboronic acid is 1.5:0.15-0.3, and the concentration of the compound A in water is 0.01 g / mL.

[0011] Furthermore, in step (2), the reaction time is 24-28 hours.

[0012] Furthermore, in step (2), the molecular weight cut-off of the dialysis bag is 2-5 kDa.

[0013] Furthermore, the preparation of the soybean peptide comprises the following steps: The soybean protein isolate was mixed with phosphate buffer, and trypsin was added for enzymolysis. After the enzymolysis, the enzyme was inactivated at 90°C for 15 minutes, the pH was adjusted to neutral, and then centrifuged to obtain soybean peptides.

[0014] A method for preparing the above-mentioned hand sanitizer comprises the following steps: Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

[0015] Furthermore, the pH is 6.5-6.8.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides a hand sanitizer, which has excellent bactericidal effect and long action time by adding modified soybean peptide and artemisia polysaccharide, and is mild, skin-friendly and non-irritating. The modified soybean peptide is reacted with the epoxy group of glycidyl methacrylate to obtain compound A, and then the amino group of compound A forms a Schiff base with 4-formylphenylboronic acid, which not only introduces antibacterial boronic acid groups, but also enhances film-forming properties, thereby achieving long-term antibacterial and skin-friendly moisturizing. Artemisia polysaccharide, as a natural extract, can reduce the irritation of the boronic acid group and synergistically enhance the antibacterial and moisturizing properties in the hand sanitizer.

[0017] 2. The present invention provides a method for preparing a wash-free hand sanitizer, which is simple and feasible and provides a new idea for preparing a new type of wash-free hand sanitizer. DETAILED DESCRIPTION

[0018] Below, in conjunction with specific embodiments, the present invention is further described. It should be noted that, under the premise of no conflict, the embodiments described below or the technical features can be arbitrarily combined to form new embodiments. The specific conditions not specified in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0019] The preparation process of soybean peptide in the present invention comprises the following steps: Soy protein isolate and phosphate buffer solution were mixed, wherein the mass fraction of soy protein isolate was 5%, and then trypsin was added thereto, wherein the amount of trypsin added was 6% of the substrate; the pH was adjusted to 8.0, and then enzymatic hydrolysis was performed at 45°C for 4 hours, heated to 90°C for 15 minutes, the enzyme was inactivated, the pH value of the enzymatic hydrolyzate was adjusted to 7.0, and then centrifuged at 5000 r / min for 20 minutes, the supernatant was taken, and freeze-dried to obtain soybean peptides.

[0020] The preparation process of artemisia polysaccharide in the present invention comprises the following steps: (1) Place dried Artemisia japonica leaves in a container, add 95% ethanol in an amount 10 times the weight of Artemisia japonica, and boil twice, each time for 3 hours, to obtain defatted Artemisia japonica; (2) Add an equal amount of distilled water to the defatted Artemisia japonica, and boil for three times, each time for 3 hours. Combine the three decoctions and concentrate to 40% to 50% of their volume. Add 3 times the volume of 95% ethanol to precipitate the product. Filter to obtain a solid product, and dry it to obtain Artemisia japonica polysaccharide.

[0021] Example 1 A hand sanitizer comprising the following ingredients by mass percentage: 0.75% modified soybean peptide, 1% artemisia polysaccharide, 2% glycerol, 0.4% carbomer, 0.10% triethanolamine, 0.3% vitamin E, 0.04% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is as follows: .

[0022] The preparation method of the modified soybean peptide is as follows: (1) Dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to react at 50° C. for 24 hours, wherein the amount ratio of the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide is 5 g:1.5 mL:1 mL:2.5 g; the concentration of the soybean peptide solution is 0.01 g / mL; adding anhydrous ethanol for precipitation, and obtaining compound A after centrifugation; (2) Compound A was added into water and stirred evenly, and 4-formylphenylboronic acid was added to react at room temperature for 26 hours. The mass ratio of compound A to 4-formylphenylboronic acid was 1.5:0.2, and the concentration of compound A in water was 0.01 g / mL. After the reaction, the reaction solution was transferred into a dialysis bag (with a molecular weight cutoff of 2 kDa), and then freeze-dried after dialysis to obtain the modified soybean peptide.

[0023] This embodiment 1 also provides a method for preparing the above-mentioned hand sanitizer, comprising the following steps: Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH to 6.5, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

[0024] Example 2 A wash-free hand sanitizer, composed of the following ingredients by mass percentage: 0.5% modified soybean peptide, 0.8% artemisia polysaccharide, 1% glycerol, 0.3% carbomer, 0.05% triethanolamine, 0.1% vitamin E, 0.02% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is the same as that of Example 1.

[0025] The preparation method of the modified soybean peptide is as follows: (1) Dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to react at 45° C. for 26 hours, wherein the amount ratio of the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide is 5 g:1 mL:0.5 mL:2 g; the concentration of the soybean peptide solution is 0.01 g / mL; adding anhydrous ethanol for precipitation, and obtaining compound A after centrifugation; (2) Compound A was added into water and stirred evenly, and 4-formylphenylboronic acid was added to react at room temperature for 24 hours. The mass ratio of compound A to 4-formylphenylboronic acid was 1.5:0.15, and the concentration of compound A in water was 0.01 g / mL. After the reaction, the reaction solution was transferred into a dialysis bag (with a molecular weight cutoff of 3 kDa), and then freeze-dried after dialysis to obtain the modified soybean peptide.

[0026] This embodiment 2 also provides a method for preparing the above-mentioned hand sanitizer, comprising the following steps: Add modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH to 6.7, then add glycerin, vitamin E and rose essence, mix well, and obtain the product.

[0027] Example 3 A wash-free hand sanitizer, composed of the following ingredients by mass percentage: 1% modified soybean peptide, 1.2% artemisia polysaccharide, 3% glycerol, 0.5% carbomer, 0.15% triethanolamine, 0.5% vitamin E, 0.05% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is the same as that of Example 1.

[0028] The preparation method of the modified soybean peptide is as follows: (1) Dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to react at 55° C. for 26 hours, wherein the amount ratio of the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide is 5 g:2 mL:1.5 mL:3 g; the concentration of the soybean peptide solution is 0.01 g / mL; adding anhydrous ethanol for precipitation, and obtaining compound A after centrifugation; (2) Compound A was added into water and stirred evenly, and 4-formylphenylboronic acid was added to react at room temperature for 28 hours. The mass ratio of compound A to 4-formylphenylboronic acid was 1.5:0.3, and the concentration of compound A in water was 0.01 g / mL. After the reaction, the reaction solution was transferred into a dialysis bag (with a molecular weight cutoff of 5 kDa), and then freeze-dried after dialysis to obtain the modified soybean peptide.

[0029] This embodiment 3 also provides a method for preparing the above-mentioned hand sanitizer, comprising the following steps: Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH to 6.8, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

[0030] Comparative Example 1 This comparative example 1 is basically the same as example 1, except that the modified soybean peptide is replaced by soybean peptide.

[0031] Comparative Example 2 This comparative example 2 is basically the same as example 1, except that the modified soybean peptide is replaced by compound A obtained in step (1).

[0032] Comparative Example 3 This comparative example 3 is substantially the same as Example 1, except that artemisia japonica polysaccharide is omitted.

[0033] Effect example 1. Antibacterial test In order to evaluate the long-term antibacterial effect of the hand sanitizer of the present invention, the following test was performed, and the specific steps were as follows: healthy female volunteers aged 25-40 were selected as subjects and randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 10 people in each group. The left and right hands of the subjects were sampled by the cotton test smearing method as a control group before the experiment. Take 2mL of the hand sanitizer prepared by Examples 1-3 and Comparative Examples 1-3 and evenly apply it to both hands (such as Example 1 group, the hand sanitizer prepared by Example 1 used), and then rub the hands for 2 minutes according to the 6-step method. Then, at 2h, 6h, and 8h after rubbing the hands, the fingers of each group of subjects were sampled by the cotton test smearing method. The specific sampling method of the above cotton swab smear method is as follows: the subject puts five fingers together, soaks the sterile cotton swab in sterile saline, and rubs it twice on the curved surface of the fingers of both hands (30 cm² for each hand) from the base to the fingertips. After rotating the swab, cut off the part that contacts the hand of the sampler, put the cotton swab into a test tube containing a neutralizer and vibrate 80 times for neutralization for 10 minutes. Take 0.5mL of the test solution and add it to a 4.5mL nutrient broth tube, incubate it in a 37℃ incubator for 48h, and calculate the total number of bacterial colonies per square centimeter of the hand. The results are detailed in Table 1. Antibacterial rate = (total number of colonies in the control group before the experiment - number of bacterial colonies in each group after different time of action) / total number of colonies in the control group before the experiment × 100%.

[0034] Table 1 Antibacterial effect of hand sanitizers in each group According to the test results in Table 1, the antibacterial effect of the hand sanitizer of Examples 1-3 of the present invention is better than that of Comparative Examples 1-3, and it still has a good antibacterial effect after 8 hours of action, and the antibacterial rate is still above 93%. Comparative Example 1 replaces the modified soybean peptide with soybean peptide, and Comparative Example 2 replaces the modified soybean peptide with compound A obtained in step (1), and Comparative Example 3 omits artemisia polysaccharide. The antibacterial effects of Comparative Examples 2 and 3 are slightly higher than those of Comparative Example 1, but both are lower than those of Example 1. This is mainly due to the addition of modified soybean peptides, indicating that the modified soybean peptides have good antibacterial effects and can inhibit the reproduction of bacteria. This is because the carboxyl group in the modified soybean peptide and the epoxy group in glycidyl methacrylate undergo a ring-opening reaction to obtain compound A, which is then obtained by the Schiff base reaction of the amino group on the soybean peptide in compound A with 4-formylphenylboronic acid. The introduced boric acid group enhances the antibacterial property of the hand sanitizer, and the introduction of methacrylate improves the film-forming property of the hand sanitizer, making it easy to form a film on the hands and not easy to lose, thereby achieving a long-lasting antibacterial effect. In addition, the addition of Artemisia japonica polysaccharide to the hand sanitizer reduces the irritation of the boric acid group in the modified soybean peptide to the skin, and can also synergize with the modified soybean peptide to enhance the antibacterial properties of the hand sanitizer.

[0035] 2. Moisturizing effect test Healthy female volunteers aged 25-40 were selected and randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 5 people in each group. After washing the inner side of the left arm with clean water, the volunteers in each group wiped it dry with sterile paper, and selected a 4 cm × 4 cm area on the inner side of the left arm as the test area, and then used a skin moisture meter to measure the stratum corneum water content of the area (i.e., the water content before the test), and then applied 0.5 mL of the hand sanitizer of the corresponding group (such as the hand sanitizer prepared in Example 1 applied in Example 1) until it was evenly absorbed, and the stratum corneum water content of the area was measured after 30 minutes. The test results are recorded in Table 2.

[0036] Table 2 Moisturizing effect of each group of hand sanitizers It can be seen from the test results in Table 2 that the wash-free hand sanitizers of Examples 1-3 of the present invention have good moisturizing effects. Compared with Example 1, the moisturizing effect of Comparative Example 3 after omitting Artemisia japonica polysaccharide is significantly reduced, and the moisturizing effect of Comparative Example 2 is slightly better than that of Comparative Example 1. This may be because Compound A in Comparative Example 2 introduces methyl methacrylate, which improves the film-forming property of the hand sanitizer, making it difficult for water to be lost, thereby improving the moisturizing effect of the hand sanitizer.

[0037] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A hand sanitizer, characterized in that: The composition is composed of the following ingredients by mass percentage: 0.5-1% modified soybean peptide, 0.8-1.2% artemisia polysaccharide, 1-3% glycerol, 0.3-0.5% carbomer, 0.05-0.15% triethanolamine, 0.1-0.5% vitamin E, 0.02-0.05% rose essence, and the balance is distilled water; the structural formula of the modified soybean peptide is as follows: 。 2. The hand sanitizer according to claim 1, characterized in that: The preparation method of the modified soybean peptide is as follows: (1) dissolving soybean peptide in water to obtain a soybean peptide solution, adding glycidyl methacrylate, triethylamine and tetrabutylammonium bromide to heat for reaction, adding anhydrous ethanol to precipitate, and centrifuging to obtain compound A; (2) Compound A is added into water and stirred evenly, and 4-formylphenylboronic acid is added to react. After the reaction is completed, the reaction solution is transferred into a dialysis bag, and after dialysis, freeze-dried to obtain a modified soybean peptide.

3. The hand sanitizer according to claim 2, characterized in that: In step (1), the soybean peptide, glycidyl methacrylate, triethylamine and tetrabutylammonium bromide are used in a ratio of 5 g: 1-2 mL: 0.5-1.5 mL: 2-3 g; The concentration of the soybean peptide solution is 0.01 g / mL.

4. The hand sanitizer according to claim 2, characterized in that: In step (1), the heating reaction temperature is 45-55°C and the time is 22-26 hours.

5. The hand sanitizer according to claim 2, characterized in that: In step (2), the mass ratio of the compound A to 4-formylphenylboronic acid is 1.5:0.15-0.3, and the concentration of the compound A in water is 0.01 g / mL.

6. The hand sanitizer according to claim 2, characterized in that: In step (2), the reaction time is 24-28 hours.

7. The hand sanitizer according to claim 2, characterized in that: In step (2), the molecular weight cutoff of the dialysis bag is 2-5 kDa.

8. The hand sanitizer according to claim 2, characterized in that: The preparation of the soybean peptide comprises the following steps: The soybean protein isolate was mixed with phosphate buffer, and trypsin was added for enzymolysis. After the enzymolysis, the enzyme was inactivated at 90°C for 15 minutes, the pH was adjusted to neutral, and then centrifuged to obtain soybean peptides.

9. A method for preparing the hand sanitizer according to any one of claims 1 to 8, characterized in that: The steps include: Add the modified soybean peptide, artemisia polysaccharide and carbomer into water according to the above weight parts, stir until fully dissolved, add triethanolamine to adjust the pH, then add glycerol, vitamin E and rose essence, mix well, and obtain the product.

10. The method for preparing the hand sanitizer according to claim 9, characterized in that: The pH is 6.5-6.8.

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