Multi-dimensional synergistic acne removal composition as well as preparation method and application thereof

Abstract

The invention relates to a multi-dimensional synergistic acne removal composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a multi-dimensional synergistic acne removal composition. The multi-dimensional synergistic acne removal composition is prepared from Shangjiang, hydrolyzed sericin, a lactobacillus / pomegranate fruit fermentation product extract, a white willow bark fermentation product and quaternary ammonium salt-73. According to the invention, Shangjiang, hydrolyzed sericin, lactobacillus / pomegranate fruit fermentation product extract, white willow bark fermentation product and quaternary ammonium salt-73 are scientifically compounded to construct a unique'prevention-treatment-repair 'three-in-one synergistic network; the five components respectively aim at four key paths of acne morbidity, namely bacterial proliferation, abnormal hair follicle keratinization, excessive sebum secretion and inflammatory response, full-path coverage from pathogenesis blocking to symptom relieving to barrier repairing is realized, and the synergistic acne removal effect is obviously superior to that of a traditional acne removal product only aiming at a single link.

Description

A multi-dimensional synergistic acne-removing composition, its preparation method and application Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a multi-dimensional synergistic acne-removing composition, its preparation method, and its application. Background Technology

[0002] Acne is a common chronic inflammatory disease of the pilosebaceous unit, primarily affecting adolescents but potentially persisting into adulthood. According to data from the *International Annals of Dermatology (2025 Edition)*, the number of consumers with acne-prone skin globally increased by 48.6% compared to 2020, with recurrent acne due to barrier damage accounting for as much as 42% of these cases. The pathogenesis of acne is complex, primarily related to multiple factors including excessive sebum secretion, abnormal follicular keratinization, excessive proliferation of *Propionibacterium acnes*, and inflammatory responses.

[0003] Currently, there are numerous acne treatment products on the market, but most only target a single aspect of the acne pathogenesis. For example, single-ingredient products containing salicylic acid or high-concentration acid products, while able to unclog pores, may damage the skin barrier and cause irritation. A member of the Dermatology Branch of the Chinese Medical Association pointed out in the "2025 China Oily Acne Skin Ingredient Care White Paper" that 85% of current ingredients fall into the "concentration superstition" misconception, blindly pursuing high concentrations of salicylic acid and niacinamide while ignoring the logic of compound formulation. This leads to 72% of users experiencing the problem of "blackheads not being cleared, but pores becoming larger." Alternatively, using strong antibacterial products such as benzoyl peroxide, while capable of killing Propionibacterium acnes, can cause dry skin, irritation, and even peeling. These products can only be used as medications, not skincare products, and long-term use can lead to drug resistance. Existing acne treatment products have significant limitations in practical application. Therefore, there is an urgent need in this field to develop an acne treatment composition that can target the acne pathogenesis in multiple dimensions and along all pathways, while also being both highly effective and gentle. Summary of the Invention

[0004] The purpose of this invention is to provide a multi-dimensional synergistic acne-removing composition, its preparation method, and its application. This invention scientifically combines Soothing Agent, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 to construct a unique "prevention-treatment-repair" three-in-one synergistic network. The five components target the four key pathways of acne pathogenesis—bacterial proliferation, abnormal follicular keratinization, excessive sebum secretion, and inflammatory response—achieving full pathway coverage from blocking the cause to symptom relief and then to barrier repair. Its synergistic acne-removing effect is significantly better than traditional acne products that only target a single aspect.

[0005] The objective of this invention can be achieved through the following technical solution: a multi-dimensional synergistic acne-removing composition, wherein the multi-dimensional synergistic acne-removing composition comprises Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73; the weight ratio of Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 is (0.5-2):(0.5-5):(0.5-2.5):(1-7):(0.01-0.1).

[0006] Furthermore, the weight ratio of the aforementioned skin-nourishing agent, hydrolyzed sericin, lactobacillus / pomegranate fruit fermentation product extract, white willow bark extract, and quaternary ammonium salt-73 is (1-1.5):(3-4):(1.5-2):(5-6):(0.03-0.07).

[0007] Further, the preparation of the white willow bark fermentation product specifically includes the following steps: S1, take dried white willow bark and pulverize it through a 40-60 mesh, mix the obtained white willow bark powder with an ethanol solution, reflux extract at 70-80℃ for 3-6 hours, filter, and concentrate to 1 / 5 of the original volume to obtain a crude extract of white willow bark; S2, add lactobacillus / pomegranate fruit fermentation product extract to the crude extract of white willow bark, adjust the pH to 5.5-6.0, and obtain the fermentation base liquid; S3, to S4. Inoculate the fermentation base liquid with *Lactobacillus plantarum* culture and ferment aerobicly at 37°C for 10-14 hours to obtain the first fermentation product; S5. Add *Lactobacillus* / pomegranate fruit fermentation product extract to the first fermentation product and ferment aerobicly at 37°C for 38-58 hours to obtain the second fermentation product; S6. Inoculate the second fermentation product with *Saccharomyces cerevisiae* culture and ferment aerobicly at 28°C for 48-72 hours, then stop the fermentation and filter with a 0.22μm filter membrane to remove bacteria to obtain white willow bark fermentation product.

[0008] Further, in step S1, the ratio of white willow bark powder to ethanol solution is 1g:(10-15)mL; the mass concentration of the ethanol solution is 55%-65%v / v.

[0009] Furthermore, the amount of Lactobacillus / pomegranate fruit fermentation product extract added in step S2 accounts for 5%-10% v / v of the crude extract of white willow bark.

[0010] Further, in step S3, the inoculation amount of *Lactobacillus plantarum* bacterial solution is 2%-4% v / v of the fermentation base liquid; the effective viable count of *Lactobacillus plantarum* in the bacterial solution is 1.2 × 10⁻⁶. 9 -1.6×10 9 CFU / mL.

[0011] Furthermore, the amount of Lactobacillus / pomegranate fruit fermentation product extract added in step S4 accounts for 2%-5% v / v of the first fermentation product.

[0012] Further, in step S5, the inoculation amount of the brewer's yeast culture is 0.6%-1.4% v / v of the second fermentation product; the effective viable count of brewer's yeast in the brewer's yeast culture is 0.8 × 10⁻⁶. 9 -1×10 9 CFU / mL.

[0013] Furthermore, the preparation method of the multi-dimensional synergistic acne-removing composition includes the following steps: mixing the following ingredients evenly by weight: Soothing agent, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 to obtain the multi-dimensional synergistic acne-removing composition.

[0014] Furthermore, the multi-dimensional synergistic acne-removing composition is applied to skincare products, including aqueous solutions, emulsions, creams, and masks, and the amount of the composition added is 0.5%-3% of the total weight of the skincare products.

[0015] The beneficial effects of the present invention are as follows: (1) The present invention scientifically combines Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 to construct a unique "prevention-treatment-repair" three-in-one synergistic network. Among them, Shanfujun prevents the formation of Propionibacterium acnes biofilm and the development of drug resistance from the root by inhibiting bacterial quorum sensing; quaternary ammonium salt-73 provides a rapid and direct antibacterial effect; white willow bark ferment, after being transformed by a specific process, gently releases salicylic acid activity, effectively unclogging pores and regulating keratin; Lactobacillus / pomegranate fruit ferment is rich in a variety of active substances, which synergistically regulate sebum secretion and inhibit inflammatory response; hydrolyzed sericin focuses on repairing the damaged skin barrier and replenishing keratin. The five ingredients target the four key pathways of acne pathogenesis - bacterial proliferation, abnormal follicular keratinization, excessive sebum secretion and inflammatory response, respectively, achieving full pathway coverage from etiological blockage to symptom relief and barrier repair. Their synergistic acne-removing effect is significantly better than traditional acne-removing products that only target a single link.

[0016] (2) This invention creatively employs a three-step sequential fermentation process to prepare white willow bark fermentation products. First, the crude extract of white willow bark is gently extracted with a specific concentration of ethanol to maximize the retention of active ingredients such as salicin. Then, Lactobacillus / pomegranate fruit fermentation product extract is used as a nutrient matrix and fermentation guide, and the substrate is initially activated and transformed through aerobic fermentation by Lactobacillus plantarum. Next, feeding is used to extend the fermentation depth and hydrolyze macromolecules. Finally, Saccharomyces cerevisiae is introduced for esterification modification and secondary metabolism to enrich the spectrum of active substances. This process effectively converts natural salicin into salicylic acid and its derivatives with higher bioavailability and significantly reduced irritation through dual-strain synergy and segmented regulation. It combines excellent acne-removing activity with excellent skin gentleness, thus solving the technical problem of traditional high-concentration acidic ingredients being highly irritating and easily damaging the skin barrier. Detailed Implementation

[0017] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0018] All the skincare products used in the embodiments and comparative examples of this invention were QUORASTOP, purchased from Synsil (Guangzhou) Trading Co., Ltd.; the hydrolyzed sericin was InnoSeriCARE (repair type) hydrolyzed sericin, purchased from Synsil (Shanghai) Biotechnology Research Co., Ltd.; the Lactobacillus / pomegranate fruit fermentation product extract was purchased from Guangzhou Huayang Biotechnology Co., Ltd., branded as Synsil (USA); the Quaternium-73 was purchased from Synsil (Guangzhou) Trading Co., Ltd., traded as Piocin; the preservation number of the Lactobacillus plantarum was CICC 20261; the preservation number of the Saccharomyces cerevisiae was CICC 32883; all other materials and reagents used in the embodiments and comparative examples of this invention, unless otherwise specified, were commercially available.

[0019] The components and their weights of the multi-dimensional synergistic acne-removing compositions of Examples 1-9 and Comparative Examples 1-8 are shown in Table 1. Each component was weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0020] Table 1. Components and weight ratios of the composition Note: The total weight of the compositions in the examples and comparative examples in Table 1 is the same.

[0021] The preparation of the white willow bark ferment in the multi-dimensional synergistic acne-removing compositions of Examples 1-9, Comparative Examples 1-3, and Comparative Examples 6-8 specifically includes the following steps: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, and concentrated to 1 / 5 of its original volume to obtain a crude white willow bark extract; S2, 7.5% v / v of Lactobacillus / pomegranate fruit ferment extract is added to the crude white willow bark extract, and the pH is adjusted to 5.7 to obtain the fermentation base liquid; S3, 1.4 × 10⁻⁶ effective viable bacteria (3% v / v) are inoculated into the fermentation base liquid. 9 S4. A *Lactobacillus plantarum* bacterial culture of CFU / mL was aerobically fermented at 37°C for 12 h to obtain the first fermentation product; S5. *Lactobacillus* / pomegranate fruit fermentation product extract (3.5% v / v) was added to the first fermentation product, and aerobic fermentation was carried out at 37°C for 48 h to obtain the second fermentation product; S6. The second fermentation product was inoculated with 0.9 × 10⁹ viable bacteria (1% v / v) of the second fermentation product. 9 The brewed yeast culture with CFU / mL was fermented aerobicly at 28℃ for 60 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0022] The only difference between Example 6 and Example 3 is that step S3 in Example 6 specifically involves inoculating the fermentation broth with an effective viable cell count of 1.2 × 10⁻⁶ at 2% v / v. 9 The first fermentation product was obtained by aerobic fermentation of CFU / mL Lactobacillus plantarum culture at 37°C for 14 hours.

[0023] The only difference between Example 7 and Example 3 is that step S3 in Example 7 specifically involves inoculating the fermentation broth with an effective viable cell count of 1.6 × 10⁻⁶ at 4% v / v. 9 The first fermentation product was obtained by aerobic fermentation of CFU / mL Lactobacillus plantarum culture at 37℃ for 10 h.

[0024] The only difference between Example 8 and Example 3 is that step S5 in Example 8 specifically involves inoculating the second fermentation product with an effective viable count of 0.8 × 10⁶ cells / v. 9 The brewed yeast culture with CFU / mL was fermented aerobicly at 28℃ for 72 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0025] The only difference between Example 9 and Example 3 is that step S5 in Example 9 specifically involves inoculating the second fermentation product with an effective viable count of 1% v / v × 10⁻⁶ cells, which accounts for 1.4% v / v of the second fermentation product. 9 The brewed yeast culture with CFU / mL was fermented aerobically at 28℃ for 48 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0026] The only difference between Comparative Example 9 and Example 3 is that the preparation of the white willow bark ferment in Comparative Example 9 is as follows: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, and concentrated to 1 / 5 of its original volume to obtain a crude white willow bark extract; S2, 7.5% v / v of Lactobacillus / pomegranate fruit ferment extract is added to the crude white willow bark extract, and the pH is adjusted to 5.7 to obtain the fermentation base liquid; S3, 0.9 × 10⁻⁶ viable bacteria at 3% v / v are inoculated into the fermentation base liquid. 9 A CFU / mL *Lactobacillus plantarum* bacterial culture was aerobically fermented at 37°C for 12 h to obtain the first fermentation product; S4, *Lactobacillus* / pomegranate fruit fermentation product extract (3.5% v / v) was added to the first fermentation product, and aerobic fermentation was carried out at 37°C for 48 h to obtain the second fermentation product; S5, the second fermentation product was inoculated with 1.4 × 10⁻⁶ viable bacteria (1% v / v). 9 The brewed yeast culture with CFU / mL was fermented aerobicly at 28℃ for 60 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0027] The only difference between Comparative Example 10 and Example 3 is that the preparation of the white willow bark ferment in Comparative Example 10 is as follows: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, and concentrated to 1 / 5 of its original volume to obtain a crude white willow bark extract; S2, 7.5% v / v of Lactobacillus / pomegranate fruit ferment extract is added to the crude white willow bark extract, and the pH is adjusted to 5.7 to obtain the fermentation base liquid; S3, 1.4 × 10⁻⁶ effective viable bacteria are inoculated into the fermentation base liquid at a concentration of 1% v / v. 9A CFU / mL *Lactobacillus plantarum* bacterial culture was aerobically fermented at 37°C for 12 h to obtain the first fermentation product; S4, *Lactobacillus* / pomegranate fruit fermentation product extract (3.5% v / v) was added to the first fermentation product, and aerobic fermentation was carried out at 37°C for 48 h to obtain the second fermentation product; S5, the second fermentation product was inoculated with an effective viable count of 0.9 × 10⁹ CFU / mL. 9 The brewed yeast culture with CFU / mL was fermented aerobicly at 28℃ for 60 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0028] The only difference between Comparative Example 11 and Example 3 is that the preparation of the white willow bark ferment in Comparative Example 11 is as follows: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, and concentrated to 1 / 5 of its original volume to obtain a crude white willow bark extract; S2, 7.5% v / v of Lactobacillus / pomegranate fruit ferment extract is added to the crude white willow bark extract, and the pH is adjusted to 5.7 to obtain the fermentation base liquid; S3, 0.9 × 10⁻⁶ effective viable bacteria at 1% v / v are inoculated into the fermentation base liquid. 9 S1) A broth of *Saccharomyces cerevisiae* (CFU / mL) was fermented aerobically at 37°C for 12 hours to obtain the first fermentation product; S4) Lactobacillus / pomegranate fruit fermentation product extract (3.5% v / v) was added to the first fermentation product, and fermented aerobically at 37°C for 48 hours to obtain the second fermentation product; S5) The second fermentation product was inoculated with 1.4 × 10⁻⁶ viable cells (3% v / v). 9 The bacterial culture of *Lactobacillus plantarum* with a concentration of CFU / mL was fermented aerobically at 28°C for 60 hours, and then the fermentation was stopped. The culture was then filtered through a 0.22 μm filter membrane to remove bacteria, yielding fermented white willow bark.

[0029] The only difference between Comparative Example 12 and Example 3 is that the preparation of the white willow bark ferment in Comparative Example 12 is as follows: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, and concentrated to 1 / 5 of its original volume to obtain a crude white willow bark extract; S2, 7.5% v / v of Lactobacillus / pomegranate fruit ferment extract is added to the crude white willow bark extract, and the pH is adjusted to 5.7 to obtain the fermentation base liquid; S3, 1.4 × 10⁻⁶ effective viable bacteria (3% v / v) are inoculated into the fermentation base liquid. 9 The effective viable count of *Lactobacillus plantarum* culture at CFU / mL and at 1% v / v of fermentation broth was 0.9 × 10⁻⁶. 9S14. A 3.5% v / v Lactobacillus / pomegranate fruit fermentation product extract was added to the first fermentation product and fermented aerobicly at 37°C for 48 hours. Fermentation was stopped and the product was filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0030] The only difference between Comparative Example 13 and Example 3 is that the preparation of the white willow bark ferment in Comparative Example 13 is as follows: S1, dried white willow bark is pulverized through a 50-mesh sieve, and the resulting white willow bark powder is mixed with a 60% v / v ethanol solution at a material-to-liquid ratio of 1g:12.5mL. The mixture is then refluxed at 75℃ for 4.5h, filtered, concentrated to 1 / 5 of its original volume, and the pH is adjusted to 5.7 to obtain a crude white willow bark extract; S2, the crude white willow bark extract is inoculated with 1.4 × 10⁻⁶ viable bacteria at a concentration of 3% v / v. 9 A CFU / mL *Lactobacillus plantarum* culture was fermented aerobically at 37°C for 60 h to obtain the first fermentation product; S4, the first fermentation product was inoculated with an effective viable count of 0.9 × 10⁻⁶ CFU / mL. 9 The brewed yeast culture with CFU / mL was fermented aerobicly at 28℃ for 60 hours and then the fermentation was stopped. The culture was then filtered through a 0.22μm filter membrane to remove bacteria, yielding white willow bark fermentation product.

[0031] The only difference between Comparative Example 14 and Example 3 is that Comparative Example 14 uses *Lactobacillus plantarum* with accession number CICC 25125 instead of *Lactobacillus plantarum* in Example 3, while the other conditions are the same as in Example 3.

[0032] The only difference between Comparative Example 15 and Example 3 is that Comparative Example 15 uses Saccharomyces cerevisiae with accession number CGMCC No.17452 instead of Saccharomyces cerevisiae in Example 3, while the other conditions are the same as in Example 3.

[0033] The only difference between Comparative Example 16 and Example 3 is that Comparative Example 16 uses Lactobacillus fermentation lysate instead of Lactobacillus / pomegranate fruit fermentation extract in Example 3. The Lactobacillus fermentation lysate was purchased from Guangzhou Huayang Biotechnology Co., Ltd., and the brand is Synsil (USA). All other conditions are the same as in Example 3.

[0034] The only difference between Comparative Example 17 and Example 3 is that Comparative Example 17 uses pomegranate fruit extract instead of the Lactobacillus / pomegranate fruit fermentation product extract in Example 3. The pomegranate fruit extract was purchased from Guangzhou Huayang Biotechnology Co., Ltd., and the brand is Synsil (USA). All other conditions are the same as in Example 3.

[0035] 1. Irritation test: 45 healthy female volunteers aged 18-55 years were randomly divided into 9 groups. All volunteers had no history of skin disease, no known allergy to the test material, and had not used any medications or cosmetics that might affect skin reaction within at least 2 weeks prior to the test; For the closed patch test, patches with an area not exceeding 40 mm² were used. 2 A qualified spot test device with a depth of approximately 1 mm was used. 0.02±0.005 mg of the multi-dimensional synergistic acne-removing composition prepared in Examples 1-9 was placed in the spot test device. The device was then attached to the flexor side of the subject's forearm with hypoallergenic adhesive tape. After 24 hours, the spot test device was removed, and the skin reaction was observed at 1 hour and 24 hours after removal. The results were recorded according to the skin reaction grading standard in the "Cosmetic Safety Technical Specifications" (2015 edition). The scoring was divided into grades 0-4, with grade 0 being a negative reaction. The test results are shown in Table 2 below.

[0036] Table 2 Results of Irritation Tests As shown in Table 2, the multi-dimensional synergistic acne-removing compositions prepared in Examples 1-9 of this invention have mild ingredients and no adverse reactions occur during use.

[0037] 2. Acne Treatment Test Product: The multi-dimensional synergistic acne-reducing compositions prepared in Examples 1-9 and Comparative Examples 1-7 were formulated into a 3% concentration sample using deionized water. Test Method: Referring to "T / CNMIA0010-2020 Safety / Efficacy Evaluation Standard for Acne-Reducing Efficacy Skincare Products" and "T / CNMIA0012-2020 Clinical Evaluation Standard for Acne-Reducing Efficacy Skincare Products", 130 female volunteers aged 18-55 years were selected. All volunteers were diagnosed by a dermatologist with inflammatory acne and closed comedones on their faces. Investigator's Static Global The ISGA assessment score ranged from 1 to 3. All volunteers primarily had non-inflammatory acne (blackheads and whiteheads), with non-inflammatory acne factors accounting for over 70% of the total acne factors. All volunteers were not pregnant, breastfeeding, or planning to have children. The ambient temperature for this test was 21±0.5℃, and the humidity was 48±3%. Volunteers were randomly divided into 26 groups. The sample was applied once daily after cleansing, morning and evening, with a dosage of 0.5±0.05g. The sample was applied evenly to the face and massaged until absorbed. The sample was used continuously for 4 weeks. The ISGA index, total number of acne, total number of non-inflammatory acne, total number of blackheads, and total number of whiteheads were measured before and after 4 weeks of use. Simultaneously, the facial condition of the subjects was photographed using a VISIA instrument before and after 4 weeks of use. The effect on facial redness was assessed using the a* value. The improvement rate of the corresponding indicators was calculated using the following formula: Improvement rate = (Mean value before use - Mean value after use) / Mean value before use × 100%. The test results are shown in Table 3 below.

[0038] Table 3 Results of Acne Treatment Trial As shown in Table 3, the multi-dimensional synergistic acne-removing compositions in Examples 1-9 of this invention are significantly better than those in Comparative Examples 1-17 in terms of improvement in ISGA index, total number of acne lesions, total number of non-inflammatory acne lesions, total number of blackheads, whiteheads, and a* value. Furthermore, the optimal effect is achieved when the weight ratio of Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate ferment extract, white willow bark ferment, and quaternary ammonium salt-73 is (0.5-2):(0.5-5):(0.5-2.5):(1-7):(0.01-0.1). That is, the five components of Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate ferment extract, white willow bark ferment, and quaternary ammonium salt-73 work synergistically to exert multi-dimensional acne-removing effects.

[0039] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition for multi-dimensional synergistic acne treatment, characterized in that, The multi-dimensional synergistic acne-removing composition includes Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73; the weight ratio of Shanfujun, hydrolyzed sericin, Lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 is (0.5-2):(0.5-5):(0.5-2.5):(1-7):(0.01-0.1).

2. The composition for multi-dimensional synergistic acne treatment according to claim 1, characterized in that, The weight ratio of the following ingredients is (1-1.5):(3-4):(1.5-2):(5-6):(0.03-0.07).

3. The composition for multi-dimensional synergistic acne treatment according to claim 1, characterized in that, The preparation of the fermented white willow bark includes the following steps: S1, take dried white willow bark and crush it through 40-60 mesh. After mixing the obtained white willow bark powder with ethanol solution, reflux extract at 70-80℃ for 3-6 hours, filter, and concentrate to 1 / 5 of the original volume to obtain crude white willow bark extract. S2. Add Lactobacillus / pomegranate fruit fermentation product extract to the crude extract of white willow bark, adjust the pH to 5.5-6.0, and obtain the fermentation base liquid; S3. Inoculate the fermentation base liquid with Lactobacillus plantarum culture, and ferment aerobically at 37℃ for 10-14h to obtain the first fermentation product; S4. Add Lactobacillus / pomegranate fruit fermentation product extract to the first fermentation product, and ferment aerobically at 37℃ for 38-58h to obtain the second fermentation product; S5. Inoculate the second fermentation product with Saccharomyces cerevisiae culture, and ferment aerobically at 28℃ for 48-72h, then stop the fermentation, filter and sterilize using a 0.22μm filter membrane to obtain the white willow bark fermentation product.

4. The composition for multi-dimensional synergistic acne removal according to claim 3, characterized in that, In step S1, the ratio of white willow bark powder to ethanol solution is 1g:(10-15)mL; the mass concentration of the ethanol solution is 55%-65%v / v.

5. The composition for multi-dimensional synergistic acne treatment according to claim 3, characterized in that, The amount of Lactobacillus / pomegranate fruit fermentation product extract added in step S2 is 5%-10% v / v of the crude extract of white willow bark.

6. The composition for multi-dimensional synergistic acne treatment according to claim 3, characterized in that, The inoculation amount of *Lactobacillus plantarum* culture in step S3 is 2%-4% v / v of the fermentation base liquid; the effective viable count of *Lactobacillus plantarum* in the culture is 1.2 × 10⁻⁶. 9 -1.6×10 9 CFU / mL.

7. The composition for multi-dimensional synergistic acne removal according to claim 3, characterized in that, The amount of Lactobacillus / pomegranate fruit fermentation product extract added in step S4 is 2%-5% v / v of the first fermentation product.

8. The composition for multi-dimensional synergistic acne removal according to claim 3, characterized in that, In step S5, the inoculation amount of the brewer's yeast culture is 0.6%-1.4% v / v of the second fermentation product; the effective viable count of brewer's yeast in the brewer's yeast culture is 0.8 × 10⁻⁶. 9 -1×10 9 CFU / mL.

9. A method for preparing a multi-dimensional synergistic acne-removing composition according to any one of claims 1-8, characterized in that, The preparation method of the multi-dimensional synergistic acne-removing composition includes the following steps: mixing the following ingredients evenly by weight: styrax, hydrolyzed sericin, lactobacillus / pomegranate fruit ferment extract, white willow bark ferment, and quaternary ammonium salt-73 to obtain the multi-dimensional synergistic acne-removing composition.

10. The application of a multi-dimensional synergistic acne-removing composition prepared by the method according to claim 9, characterized in that, The multi-dimensional synergistic acne-removing composition is applied to skincare products, including aqueous solutions, emulsions, creams, and masks, and the amount of the composition added is 0.5%-3% of the total weight of the skincare products.

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