Composition for synergistically relieving physical fatigue and application thereof

Abstract

The invention discloses a composition for synergistically relieving physical fatigue and application thereof. The composition disclosed by the invention can synergistically regulate oxidation resistance, calcium ion metabolism and energy metabolism, synergistically improve the ATP content and glycogen level of an organism, accelerate removal of lactic acid and urea nitrogen, prolong exercise time and remarkably relieve physical fatigue, and the synergistic effect of the composition is better than that of single use of red ginseng, lycium ruthenicum and lucid ganoderma. Meanwhile, the components of the composition disclosed by the invention are simple and are all from natural plants; meanwhile, through scientific argumentation and test verification, the action mechanism of the composition is clear, the fatigue relieving interaction relationship among the components is clear, and the composition has the advantages of being green and safe. In addition, the composition disclosed by the invention is simple in preparation process, convenient to operate, low in production cost and convenient to realize industrial development, and professional technicians and large-scale instruments and equipment do not need to be invested.

Description

Technical Field

[0001] The present invention relates to the field of medicine and food technology, and in particular to a composition for synergistically alleviating physical fatigue and an application thereof. Background Art

[0002] Fatigue is a common physiological phenomenon in human life, and sports fatigue refers to the phenomenon that in the physiological process, one cannot continue to function at a specific level or cannot maintain a predetermined exercise intensity. Some food-derived active ingredients can significantly regulate the body's energy metabolism and metabolite accumulation and relieve physical fatigue through multiple pathways such as anti-oxidation, reducing metabolite accumulation, anti-inflammation, regulating energy metabolism and intestinal microecology.

[0003] In recent years, research in the field of anti-fatigue has made certain progress at home and abroad, but there are still many unresolved problems. For example, although some known active ingredients have certain anti-fatigue effects, their effects are limited and there may be certain side effects. In addition, the formula design and preparation process of existing products also have certain limitations, which are difficult to meet the needs of the market, such as: the formula ingredients are complex, and the selection and ratio of raw materials lack scientific basis; the mechanism of action of compound formula products is unclear; most products are formulated based on practical application experience, lack scientific demonstration and experimental verification; the interaction relationship between the various ingredients in the formula to relieve fatigue is unclear. With the continuous improvement of consumers' health awareness, it is easier to get people's recognition to develop products with few side effects and health care functions for the human body from natural plants, especially traditional edible resources. Therefore, it is of great significance to develop a scientifically verified, safe, and directly edible product composed of traditional medicinal and edible products or active ingredients from natural plants with a synergistic effect of relieving physical fatigue.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The object of the present invention is to provide a composition for synergistically relieving physical fatigue and its application. The composition is composed of at least two of red ginseng, black wolfberry and ganoderma lucidum, and is compounded in a specific ratio so that it can effectively relieve physical fatigue.

[0006] To achieve the above purpose, the technical solution adopted by the present invention to solve the technical problem is: The invention provides a traditional Chinese medicine composition, the components of which are selected from at least two of red ginseng, black wolfberry and ganoderma lucidum.

[0007] Red Ginseng( Talinum paniculata(Jacq.) Gaertn.) is a perennial herb of the genus Panax in the Araliaceae family. It is a cooked product of ginseng. The lateral roots and fine roots of ginseng are removed and steamed, sun-dried or baked. It has a variety of effective ingredients, such as ginsenosides, polyacetylene compounds and acidic polysaccharides. Black wolfberry, also known as Su wolfberry, is a plant of the genus Lycium in the Solanaceae family. It is sweet and flat in nature and enters the liver and kidney meridians. Ganoderma lucidum is a traditional tonic Chinese medicine, and Ganoderma lucidum polysaccharide is one of its main active ingredients.

[0008] The present invention screens out a batch of substances that can relieve physical fatigue from a variety of food-derived ingredients, and by analyzing and comparing the effects of various substances on indicators such as the body's ATP content, glycogen level, lactic acid and urea nitrogen removal ability, it is found that red ginseng can play an anti-fatigue effect by regulating mouse serum urea nitrogen and body glycogen content; the main active ingredient in black wolfberry-black wolfberry polysaccharide can improve serum and liver superoxide dismutase activity, enhance the body's antioxidant capacity, and relieve mouse fatigue; Ganoderma lucidum polysaccharide in Ganoderma lucidum can increase mouse glycogen reserves, reduce lactic acid and urea nitrogen accumulation, and relieve fatigue. Ganoderma lucidum triterpenes have strong antioxidant and anti-inflammatory abilities, and can relieve fatigue by regulating body metabolism. Therefore, the three substances of red ginseng, black wolfberry and Ganoderma lucidum are selected to be compounded in pairs or three kinds. The composite composition can play a synergistic effect in terms of body ATP content, glycogen level, lactic acid and urea nitrogen removal ability, so that the anti-fatigue effect after compounding is significantly improved.

[0009] Based on this, the composition provided by the present invention can be a composition formed by two components: red ginseng and black wolfberry, red ginseng and ganoderma lucidum, black wolfberry and ganoderma lucidum, or a composition formed by three components: red ginseng, black wolfberry and ganoderma lucidum.

[0010] The forms of the above ingredients include but are not limited to powders, and may also be extracts, extracts, concentrates and slices. Specifically, the extracts may be water extracts or alcohol extracts, and those skilled in the art may make compounding adjustments based on actual conditions.

[0011] In some embodiments, when preparing the above composition, red ginseng, black wolfberry and ganoderma lucidum are all processed into powder state for compounding.

[0012] The present invention does not limit the sources, preparation or extraction methods of the above components, and they can be purchased from the market or prepared by conventional processing methods in the art.

[0013] In order to obtain better effects, the present invention also conducts experimental verification on the addition ratios of different components in each combination, and the results show that the effects of the compositions at different ratios are significantly different.

[0014] In some embodiments, the composition is composed of the following components and their weight parts by weight: 2-8 parts of red ginseng powder and 1-9 parts of black wolfberry powder. Specifically, the ratio of red ginseng powder to black wolfberry powder can be: 2:1, 2:9, 3:1, 3:9, 4:1, 4:9, 5:1, 5:9, 6:1, 6:9, 7:1, 7:9, 8:1 or 8:9, etc., or any other ratio between 2-8:1-9.

[0015] In some embodiments, the composition is composed of the following components and their weight parts by weight: 2-8 parts of red ginseng powder and 3-10 parts of ganoderma lucidum powder. Specifically, the ratio of red ginseng powder to ganoderma lucidum powder can be: 2:3, 2:10, 3:3, 3:10, 4:6, 4:10, 5:3, 5:10, 6:3, 6:10, 7:3, 7:10, 8:3 or 8:10, etc., or any other ratio between 2-8:3-10.

[0016] In some embodiments, the composition is composed of the following components and their weight parts by weight: 1-9 parts of black wolfberry powder and 3-10 parts of ganoderma lucidum powder. Specifically, the ratio of black wolfberry powder to ganoderma lucidum powder can be: 1:3, 1:10, 2:3, 2:10, 3:3, 3:2, 4:3, 4:10, 5:3, 5:10, 9:3 or 9:10, etc., or any other ratio between 1-9:3-10.

[0017] In some embodiments, the composition is composed of the following components and their weight parts by weight: 2-8 parts of red ginseng powder, 1-9 parts of black wolfberry powder, and 3-10 parts of ganoderma lucidum powder. Specifically, the ratio of red ginseng powder, black wolfberry powder and ganoderma lucidum powder can be: 2:1:3, 2:1:10, 2:9:3, 2:9:10, 8:1:3, 8:1:10, 8:9:3 or 8:9:10, etc., or any other ratio between 2-8:1-9: 3-10.

[0018] In some embodiments, the composition is composed of the following components and their weight parts by weight: 2 parts of red ginseng powder, 3 parts of black wolfberry powder, and 8 parts of ganoderma lucidum powder. In some animal experiments, it can be found that the composition of the present invention has the best regulating effect on the body's antioxidant, calcium ion metabolism, energy metabolism, and glycogen level when the mass ratio is 2:3:8, and the combined index CI value is lower, which further indicates that under the above ratio conditions, the composition can more effectively relieve physical fatigue.

[0019] The combination index method is a method for evaluating the combined effects of drugs. It is particularly suitable for evaluating the interaction between two or more ingredients after compounding. This method is based on the dose-effect curve of the drug. By calculating the ratio of the actual effect to the theoretical effect during combined use, it is determined whether the combined use has a synergistic effect, an additive effect, or an antagonistic effect. A combination index (CI) less than 1 indicates a synergistic effect, 1 indicates an additive effect, and greater than 1 indicates an antagonistic effect. Therefore, through experiments to screen out food-derived ingredients that are better at relieving physical fatigue, and combining the combination index method to evaluate the interaction relationship between food functional ingredients, it is very beneficial to the development of synergistic functional food formulas.

[0020] The present invention verifies the effect of the above scheme by the combined index method, and the results show that no matter it is a composition formed by two components or a composition formed by three components, it can improve the swimming distance, recovery rate and ATP, reduce the lactic acid content, and increase the expression of Nrf2, atp2b1 and g6pca genes. Among them, the Nrf2 gene is an oxidative stress-related gene, the atp2b1 gene is a calcium ion metabolism-related gene, and the g6pca gene is an energy metabolism-related gene. By detecting the expression changes of these three genes, it can be proved that the various components in the composition of the present invention can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism pathways, thereby relieving physical fatigue.

[0021] At the same time, the three-component composition of the present invention has also been verified by the standard method of "Health Food Function Test and Evaluation Method (2023 Edition)". The results show that the composition formed by the three ingredients can prolong swimming time, reduce lactic acid and urea nitrogen content, and increase muscle glycogen and liver glycogen content. Mammalian experiments further prove that the three-component composition has anti-fatigue effects.

[0022] Based on the above, the present invention can also provide the use of the above composition in the preparation of a product for relieving physical fatigue; or, the present invention can also provide the use of the above composition in the preparation of a product for improving ATP energy metabolism or reducing lactic acid; or, the present invention can also provide the use of the above composition in the preparation of a product for regulating the expression of Nrf2, atp2b1 and g6pca genes.

[0023] The composition of the present invention has broad application prospects and can be widely used in the fields of food, health care products, medicines, etc. In this regard, the present invention can also provide a food or health care product, the active ingredient of which is the above composition.

[0024] In some embodiments, the composition of the present invention can be made into food using techniques known to those skilled in the art.

[0025] In some embodiments, the food or health product prepared from the composition of the present invention may further include food-acceptable auxiliary materials, such as solvents, adhesives, fillers, sugars, sweeteners, and the like.

[0026] In some embodiments, the food or health product prepared from the composition of the present invention can be of the following types: tablets, capsules, oral liquids, solid beverages, pills, granules, decoctions, pastes, lotions, pills, syrups or tea bags.

[0027] In some embodiments, the compositions of the present invention can be formulated into medicines suitable for topical administration using techniques known to those skilled in the art.

[0028] In some embodiments, the drug prepared by the composition of the present invention may also include pharmaceutically acceptable excipients, such as: buffers, emulsifiers, suspending agents, decomposers, disintegrants, dispersants, binders, excipients, stabilizers, chelating agents, diluents, gelling agents, preservatives, wetting agents, lubricants, absorption delaying agents, liposomes, etc.

[0029] In some embodiments, the drug prepared from the composition of the present invention can be in the following dosage forms: tablets, oral liquids, hard capsules, soft capsules, granules, powders or pills.

[0030] In the above-mentioned foods and medicines, the addition amount and addition ratio of the composition of the present invention and related auxiliary materials can be adjusted according to actual needs, and the present invention does not limit this.

[0031] The present invention has the following beneficial effects: The present invention compounds red ginseng, black wolfberry and ganoderma lucidum, and the obtained various compositions can synergistically regulate anti-oxidation, calcium ion metabolism and energy metabolism, synergistically improve the body's ATP content and glycogen level, accelerate the removal of lactic acid and urea nitrogen, prolong exercise time, and significantly relieve physical fatigue. Its synergistic effect is stronger than that of using red ginseng, black wolfberry and ganoderma lucidum alone. At the same time, the composition of the present invention is simple in composition, and all are derived from natural plants; at the same time, after scientific demonstration and experimental verification, the composition has a clear mechanism of action, and the fatigue-relieving interaction relationship between the components is clear, and it has the advantages of being green and safe. In addition, the preparation process of the composition of the present invention is simple, easy to operate, does not need to invest in professional and technical personnel and large-scale instruments and equipment, has a low production cost, and is easy to achieve industrial development. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0033] The present invention is further described below by means of specific examples. However, it should be understood that these examples are only used for more detailed description and should not be construed as limiting the present invention in any form.

[0034] The red ginseng, black wolfberry and ganoderma lucidum used in the embodiments and comparative examples of the present invention are purchased from the market, and the preparation method thereof is as follows: appropriately weigh the qualified red ginseng, black wolfberry and ganoderma lucidum respectively, use a grinder to grind them into large particles, then place them in a multifunctional extraction tank respectively, add water to extract for 4 hours, filter the filtrate through 100 mesh, reduce the pressure on the filtered filtrate and concentrate it to a relative density of 1.06, spray dry it to obtain a dry powder, and after the dry powder is crushed, pass it through an 80-mesh sieve to obtain dry powder of each component for standby use.

[0035] Example 1 This embodiment is a composition, and its components are as follows: 3 parts of red ginseng powder and 4 parts of black wolfberry powder.

[0036] Example 2 This embodiment is a composition, and its components are as follows: 2 parts of red ginseng powder and 9 parts of black wolfberry powder.

[0037] Example 3 This embodiment is a composition, and its components are as follows: 8 parts of red ginseng powder and 1 part of black wolfberry powder.

[0038] Example 4 This embodiment is a composition, and its components are as follows: 3 parts of red ginseng powder and 4 parts of ganoderma lucidum powder.

[0039] Example 5 This embodiment is a composition, the components of which are as follows: 1 part of red ginseng powder and 5 parts of ganoderma lucidum powder.

[0040] Example 6 This embodiment is a composition, and its components are as follows: 8 parts of red ginseng powder and 3 parts of ganoderma lucidum powder.

[0041] Example 7 This embodiment is a composition, the components of which are as follows: 3 parts of black wolfberry powder and 4 parts of ganoderma lucidum powder.

[0042] Example 8 This embodiment is a composition, the components of which are as follows: 1 part of black wolfberry powder and 10 parts of ganoderma lucidum powder.

[0043] Example 9 This embodiment is a composition, the components of which are as follows: 3 parts of black wolfberry powder and 1 part of ganoderma lucidum powder.

[0044] Example 10 This embodiment is a composition, the components of which are as follows: 1.5 parts of red ginseng powder, 1.5 parts of black wolfberry powder, and 4 parts of ganoderma lucidum powder.

[0045] Embodiment 11 This embodiment is a composition, the components of which are as follows: 1.5 parts of red ginseng powder, 1.5 parts of black wolfberry powder, and 10 parts of ganoderma lucidum powder.

[0046] Example 12 This embodiment is a composition, the components of which are as follows: 3 parts of red ginseng powder, 3 parts of black wolfberry powder, and 1 part of ganoderma lucidum powder.

[0047] Example 13 This embodiment is a composition, the components of which are as follows: 2 parts of red ginseng powder, 3 parts of black wolfberry powder, and 8 parts of ganoderma lucidum powder.

[0048] Experimental Example 1 6 dpf wild-type AB strain zebrafish were selected, and then an equal amount of the composition in each embodiment was dissolved in water, and the zebrafish were placed in water containing food-derived ingredients at 27°C for 24 hours. The experimental treatments are shown in Table 1. After the zebrafish fatigue model was established by sodium sulfite induction for 30 minutes, the total movement distance of each zebrafish within 30 minutes was analyzed, the fatigue recovery rate was calculated, and the lactic acid and adenosine triphosphate (ATP) contents and related gene expression levels were determined.

[0049] The formula for calculating the recovery rate of zebrafish fry is as follows: Recovery rate = (swimming distance of treatment group - swimming distance of model group) / (swimming distance of control group - swimming distance of model group) × 100% The swimming distance of the control group was 1996.7 ± 263.5 mm. The recovery rate of zebrafish fry in each group was calculated according to the above formula. The specific results are as follows: Table 1 Effects of red ginseng powder and black wolfberry powder on zebrafish fatigue

[0050] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0051] As shown in Table 1, compared with the control group, the swimming distance of zebrafish in the model group decreased by 50.0%, indicating that sodium sulfite induced zebrafish fatigue; Red Bull, a recognized anti-fatigue health drink, was selected as the positive control group, and the swimming distance of zebrafish increased by 75.6% (p<0.001), and the swimming distance was significantly restored compared with the control group, indicating that Red Bull alleviated sodium sulfite-induced zebrafish fatigue, and the fatigue model was successfully constructed.

[0052] When red ginseng powder and black wolfberry powder were used alone, the swimming distance increased significantly (p<0.05), and the recovery rate exceeded 50%, indicating that both have good fatigue relief potential. When red ginseng powder and black wolfberry powder were used in a compound ratio of 3:4, 2:9 and 8:1, the swimming distance increased by 69.5%, 79.3%, and 65.8% (p<0.01) compared with the model group, and the recovery rates were 69.6%, 79.3%, and 65.6%, respectively. The corresponding CI values ​​were 0.53, 0.46, and 0.71, respectively, all less than 1, indicating that when the compound ratio was 2:9~8:1, red ginseng powder and black wolfberry powder could synergistically relieve physical fatigue.

[0053] When red ginseng powder was used alone, the ATP content increased (p<0.05), while there was no significant change in black wolfberry powder, indicating that red ginseng powder can increase ATP content and relieve physical fatigue. When red ginseng powder and black wolfberry powder were used in a compound ratio of 3:4, 2:9 and 8:1, the ATP content increased by 9.6%, 4.7% and 15.2%, respectively (p<0.05), and the corresponding CI values ​​were 0.74, 0.88 and 0.32, respectively, all less than 1, indicating that when the compound ratio is 2:9~8:1, physical fatigue can be significantly relieved by synergistically increasing ATP content.

[0054] In addition, red ginseng powder and black wolfberry powder can reduce lactic acid content when used alone (p<0.05), indicating that they can reduce lactic acid content in the body and relieve physical fatigue. When red ginseng powder and black wolfberry powder were used in a mixture of 3:4, 2:9 and 8:1, the lactic acid content was reduced by 22.5%, 25.0% and 17.5% respectively compared with the model group (p<0.05), and the corresponding CI values ​​were 0.77, 0.63 and 0.86, respectively, all less than 1, indicating that red ginseng powder and black wolfberry powder can synergistically reduce lactic acid content and relieve physical fatigue when mixed in a ratio of 2:9 to 8:1.

[0055] When red ginseng powder and black wolfberry powder were used alone, both increased the expression of Nrf2 gene (p<0.05), indicating that both can relieve physical fatigue by improving the body's antioxidant capacity. When red ginseng powder and black wolfberry powder were mixed at 3:4, 2:9 and 8:1, the expression of Nrf2 gene increased by 4.39, 2.78 and 2.21 times, respectively (p<0.01), and the CI values ​​were 0.32, 0.58 and 0.77, respectively, indicating that the combination of the two can relieve physical fatigue by synergistically regulating the body's antioxidant pathway. When red ginseng powder and black wolfberry powder were used alone, the expression of atp2b1 gene was increased (p<0.05). When the two were mixed at 3:4, 2:9 and 8:1, the expression of atp2b1 gene increased by 1.87, 1.51 and 2.10 times (p<0.05), and the CI values ​​were 0.87, 0.95 and 0.49, respectively, indicating that the combination relieved physical fatigue by synergistically regulating the calcium ion metabolism pathway of the body. When red ginseng powder and black wolfberry powder were used alone, the expression of g6pca gene was increased by 1.72, 1.62 and 1.97 times (p<0.05) when the two were mixed at 3:4, 2:9 and 8:1, and the CI values ​​were 0.71, 0.88 and 0.64, respectively, indicating that the combination can relieve physical fatigue by synergistically upregulating the energy metabolism pathway.

[0056] In summary, when red ginseng powder and black wolfberry powder are compounded in a ratio of 2:9~8:1, they can synergistically regulate the body's antioxidant capacity, calcium ion metabolism and energy metabolism, and synergistically increase the body's ATP content, accelerate lactic acid clearance, thereby increasing the swimming distance of zebrafish, improving the recovery rate, and significantly alleviating physical fatigue. The recovery effect is comparable to that of Red Bull.

[0057] Experimental Example 2 The experimental method was the same as that of Experimental Example 1. The compound raw materials and proportions were shown in Table 2. The swimming distance of the control group was 2020.4 ± 213.6 mm. The specific results are as follows: Table 2 Effects of red ginseng powder and ganoderma lucidum powder on zebrafish fatigue

[0058] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0059] As shown in Table 2, when red ginseng powder and ganoderma powder were used alone, they could increase the swimming distance of zebrafish (p<0.05), and the recovery rate was above 30%, which showed the potential to relieve physical fatigue. When red ginseng powder and ganoderma powder were mixed at 3:4, 1:5 and 8:3, the swimming distance increased by 53.4%, 77.8% and 67.6% (p<0.05), respectively, and the recovery rates were 49.5%, 73.3% and 63.4%, respectively. The corresponding CI values ​​were 0.79, 0.37 and 0.64, respectively, which were all less than 1, indicating that when the mixing ratio was in the range of 1:5~8:3, red ginseng powder and ganoderma powder could synergistically relieve physical fatigue.

[0060] When red ginseng powder and ganoderma lucidum powder were used alone, the ATP content increased (p<0.05), indicating that both can relieve fatigue by increasing the ATP content. After red ginseng powder and ganoderma lucidum powder were mixed at 3:4, 1:5 and 8:3, the ATP content of each group increased by 10.9%, 7.9% and 17.98% respectively (p<0.01), and the corresponding CI values ​​were 0.82, 0.91 and 0.28 respectively, all less than 1, indicating that the two can synergistically increase the ATP content and relieve fatigue.

[0061] In addition, when red ginseng powder and ganoderma powder were used alone, they could reduce lactic acid content (p<0.05) and relieve physical fatigue. When red ginseng powder and ganoderma powder were used in a mixture of 3:4, 1:5 and 8:3, lactic acid content was reduced by 23.6%, 28.9% and 18.4% (p<0.05), respectively, and the corresponding CI values ​​were 0.46, 0.44 and 0.89, respectively, all less than 1, indicating that the mixture can synergistically reduce lactic acid content and relieve physical fatigue.

[0062] When red ginseng powder and ganoderma lucidum powder were used alone, they could increase the expression of Nrf2 gene (p<0.05). When they were mixed at 3:4, 1:5 and 8:3, the expression of Nrf2 gene increased by 2.69, 1.89 and 2.83 times (p<0.01), and the CI values ​​were 0.61, 0.88 and 0.44, respectively, indicating that they could synergistically regulate the body's antioxidant pathway to relieve physical fatigue. Both red ginseng powder and ganoderma lucidum powder increased the expression of atp2b1 gene (p<0.05). When they were mixed at 3:4, 1:5 and 8:3, the expression of atp2b1 gene increased by 2.0, 1.81 and 2.1 times (p<0.01), and the CI values ​​were 0.33, 0.49 and 0.29, respectively, indicating that they could synergistically regulate the body's calcium ion metabolism and relieve physical fatigue. When red ginseng powder and ganoderma lucidum powder were used alone, the expression of g6pca gene was increased (p<0.05). When the two were mixed in a ratio of 3:4, 1:5 and 8:3, the expression of g6pca gene increased by 1.79, 1.59 and 1.94 times, respectively (p<0.05), and the CI values ​​were 0.68, 0.71 and 0.45, respectively. This shows that the combination of red ginseng powder and ganoderma lucidum powder can synergistically upregulate the body's energy metabolism pathways and relieve physical fatigue.

[0063] In summary, when red ginseng powder and ganoderma lucidum powder are mixed in a ratio of 1:5~8:3, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism, synergistically increase the body's ATP content, accelerate lactic acid clearance, increase the swimming distance of zebrafish and improve the recovery rate, relieve physical fatigue, and the recovery effect is comparable to that of Red Bull.

[0064] Experimental Example 3 The experimental method was the same as that of Experimental Example 1. The compound raw materials and their proportions are shown in Table 3. The swimming distance of the control group was 1594.8 ± 347.6 mm. The specific results are as follows: Table 3 Effects of black wolfberry powder and ganoderma lucidum powder on zebrafish fatigue

[0065] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0066] As shown in Table 3, both black wolfberry powder and ganoderma powder can increase the swimming distance of zebrafish (p<0.05), and the recovery rates of both are above 30%, and both have the potential to relieve physical fatigue. When black wolfberry powder and ganoderma powder were used in a mixture of 3:4, 1:10 and 3:1, the swimming distance increased by 54.49%, 61.0%, and 58.4% (p<0.01) compared with the model group, and the recovery rates were 55.7%, 61.0%, and 58.4%, respectively. The corresponding CI values ​​were 0.57, 0.26, and 0.69, respectively, all less than 1, indicating that black wolfberry powder and ganoderma powder can synergistically relieve physical fatigue when mixed at 1:10~3:1.

[0067] When black wolfberry powder and ganoderma powder were used alone, the ATP content of ganoderma powder increased (p<0.05), while there was no significant change in black wolfberry powder. When black wolfberry powder and ganoderma powder were mixed at a ratio of 3:4, 1:10 and 3:1, the ATP content increased by 7.9%, 13.3% and 5.4%, respectively (p<0.05), and the corresponding CI values ​​were 0.44, 0.19 and 0.51, respectively, all less than 1, indicating that when the two are mixed, they can synergistically increase the ATP content and relieve physical fatigue.

[0068] In addition, when black wolfberry powder and ganoderma powder were used alone, they could reduce lactic acid content (p<0.05) and relieve physical fatigue. When the two were used in a mixture of 3:4, 1:10 and 3:1, the lactic acid content was reduced by 20.5%, 28.2% and 23.0%, respectively (p<0.05). The corresponding CI values ​​were 0.56, 0.22 and 0.48, respectively, all less than 1, indicating that the combination of black wolfberry powder and ganoderma powder can synergistically reduce lactic acid content and relieve physical fatigue.

[0069] Both red ginseng powder and black wolfberry powder can increase the expression of Nrf2 gene when used alone (p<0.05). When the two are mixed in a ratio of 3:4, 1:10 and 3:1, the expression of Nrf2 gene increases by 3.17, 3.34 and 2.94 times, respectively (p<0.001), and the CI values ​​are 0.54, 0.39 and 0.66, respectively, indicating that the combination of red ginseng powder and black wolfberry powder can relieve physical fatigue by synergistically regulating the body's antioxidant pathways. When black wolfberry powder and ganoderma powder were used alone, the expression of atp2b1 gene was increased (p<0.05), and when the two were mixed at a ratio of 3:4, 1:10 and 3:1, the expression of atp2b1 gene increased by 2.06, 2.24 and 2.15 times, respectively (p<0.01), with CI values ​​of 0.76, 0.55 and 0.69, indicating that when red ginseng powder and black wolfberry powder are mixed, they can synergistically regulate the body's calcium ion metabolism and relieve physical fatigue. In addition, both black wolfberry powder and Ganoderma lucidum powder increased the expression of the g6pca gene (p<0.05), and after the two were mixed in a ratio of 3:4, 1:10 and 3:1, the expression of the g6pca gene increased by 1.84, 2.0 and 1.64 times, respectively (p<0.01), and the CI values ​​were 0.67, 0.43 and 0.77, respectively, indicating that when red ginseng powder and black wolfberry powder are mixed, they can synergistically up-regulate the body's energy metabolism pathways and relieve physical fatigue.

[0070] In summary, when red ginseng powder and ganoderma lucidum powder are mixed in a ratio of 1:10~3:1, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism, and synergistically increase the body's ATP content, accelerate lactic acid clearance, significantly increase the swimming distance of zebrafish and improve the recovery rate, relieve physical fatigue, and the recovery effect is comparable to that of Red Bull.

[0071] Experimental Example 4 The experimental method was the same as that of Experimental Example 1. The compound raw materials and proportions were shown in Table 3. The swimming distance of the control group was 1679.1 ± 251.3 mm. The specific results are as follows: Table 4 Effects of red ginseng powder, black wolfberry powder and ganoderma powder on zebrafish fatigue

[0072] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0073] As shown in Table 4, when red ginseng powder, black wolfberry powder and ganoderma powder were used alone, they all increased the swimming distance of zebrafish (p<0.05), and the recovery rates were 48.2%, 51.3% and 38.5%, respectively, and all had the potential to relieve physical fatigue. When red ginseng powder + black wolfberry powder and ganoderma powder were used in a compound ratio of 3:4, 3:10 and 6:1, the swimming distance increased by 77.7%, 73.5% and 74.3% respectively compared with the model group (p<0.01), and the recovery rates were 77.7%, 73.5% and 74.3%, respectively, and the corresponding CI values ​​were 0.21, 0.30 and 0.35, respectively, all less than 1, indicating that when the compound range was 3:10~6:1, red ginseng powder, black wolfberry powder and ganoderma powder could significantly relieve physical fatigue synergistically.

[0074] When red ginseng powder, black wolfberry powder and ganoderma powder were used alone, the ATP content was increased (p<0.05). When the three were mixed in the ratio of 3:4, 3:10 and 6:1, the ATP content increased by 12.1%, 17.3% and 13.5%, respectively (p<0.001). The corresponding CI values ​​were 0.43, 0.22 and 0.32, all less than 1, indicating that the combination of red ginseng powder, black wolfberry powder and ganoderma powder can synergistically increase the ATP content and relieve physical fatigue.

[0075] In addition, red ginseng powder, black wolfberry powder and ganoderma lucidum powder can reduce lactic acid content (p<0.05) and relieve physical fatigue. When the three are used in combination, the lactic acid content is reduced by 34.1%, 36.5% and 26.8% respectively compared with the model group (p<0.01). The corresponding CI values ​​are 0.38, 0.33 and 0.29, all less than 1, indicating that when red ginseng powder, black wolfberry powder and ganoderma lucidum powder are used in combination at a ratio of 3:10~6:1, they can synergistically reduce lactic acid content and relieve physical fatigue.

[0076] When red ginseng powder, black wolfberry powder and ganoderma powder were used alone, they all increased the expression of Nrf2 gene (p<0.05), indicating that the three can relieve fatigue by regulating the body's antioxidant pathway. After red ginseng powder + black wolfberry powder and ganoderma powder were mixed at 3:4, 3:10 and 6:1, the expression of Nrf2 gene increased by 3.12, 3.03 and 2.52 times (p<0.001), respectively, and the CI values ​​were 0.36, 0.46 and 0.55, respectively, indicating that the three can synergistically regulate the body's antioxidant pathway to relieve physical fatigue after mixing. Red ginseng powder, black wolfberry powder and ganoderma powder can increase the expression of atp2b1 gene (p<0.05), regulate calcium ion metabolism and relieve physical fatigue. When the three were mixed at 3:4, 3:10 and 6:1, the expression of atp2b1 gene increased by 5.17, 4.29 and 4.88 times, respectively (p<0.001), and the CI values ​​were 0.21, 0.41 and 0.33, respectively, indicating that the combination of red ginseng powder, black wolfberry powder and ganoderma powder can synergistically regulate calcium ion metabolism and relieve physical fatigue. When red ginseng powder, black wolfberry powder and ganoderma powder were used alone, they could also increase the expression of g6pca gene (p<0.05), indicating that the three can relieve physical fatigue by upregulating the energy metabolism pathway of the body. When red ginseng powder + black wolfberry powder and ganoderma lucidum powder were mixed at a ratio of 3:4, 3:10 and 6:1, the expression level of g6pca gene increased by 2.35, 2.0 and 2.25 times, respectively (p<0.001), and the CI values ​​were 0.33, 0.44 and 0.29, respectively, indicating that the combination of the three can significantly synergistically regulate the body's energy metabolism pathways and relieve physical fatigue.

[0077] In summary, when red ginseng powder + black wolfberry powder and ganoderma lucidum powder are compounded in a ratio of 3:10~6:1, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism, and synergistically increase the body's ATP content, accelerate lactic acid clearance, significantly increase the swimming distance of zebrafish and improve the recovery rate, and effectively relieve physical fatigue.

[0078] Combined with the experimental results of Experimental Examples 1 to 3, it was found that when any two of red ginseng powder, black wolfberry powder and ganoderma lucidum powder are mixed, they can synergistically relieve physical fatigue, and when the three are mixed, they can exert a stronger synergistic effect, and the recovery effect is slightly higher than Red Bull.

[0079] Experimental Example 5 The compound formula composed of red ginseng powder, black wolfberry powder and ganoderma powder in different proportions has been shown to have a significant effect in relieving physical fatigue in zebrafish experiments. However, the structure of zebrafish is quite different from that of the human body, so the mouse fatigue model experiment was used to further verify the fatigue relief effect of the formula, providing reliable data support for the subsequent product development and use. According to the compound formula dosage combination verified in the zebrafish experiment, the conversion method of zebrafish to human dosage recorded in patent CN113496071A was used to convert the compound formula dosage in the zebrafish experiment into the mouse intake dosage, and the standard method of "Health Food Function Test and Evaluation Method (2023 Edition)" was strictly followed to verify the fatigue relief effect of the compound formula. The specific experimental process is as follows: Male C57 / 6J mice weighing approximately (20±2 g) were selected, and the experimental treatments were shown in Table 5. They were gavaged for 30 consecutive days. At the end of the experimental period, a weighted swimming test was performed on mice 30 min after gavage. The time from the start of swimming to the time when the head was completely submerged in water and could not swim out of the water for 5 s was recorded as the exhaustive swimming time of the mice. The contents of lactic acid, urea nitrogen, muscle glycogen, and liver glycogen were measured, and the results are as follows.

[0080] Table 5 Effects of red ginseng powder, black wolfberry powder and ganoderma lucidum powder on fatigue in mice

[0081] Note: The addition amount of each experimental group is the same; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0082] As shown in Table 5, Red Bull, a recognized anti-fatigue health drink, was selected as the positive control group, and the exhaustive swimming time of mice was extended by 83.9% (p<0.01), indicating that Red Bull can significantly relieve the physical fatigue of mice. When red ginseng powder + black wolfberry powder and ganoderma lucidum powder were used in a compound ratio of 3:4, 3:10 and 6:1, the exhaustive swimming time was extended by 84.14%, 78.73% and 66.79% respectively (p<0.05), indicating that when the compound ratio is 3:10~6:1, the formula has the effect of relieving physical fatigue of mice.

[0083] When red ginseng powder + black wolfberry powder and ganoderma lucidum powder were used in a compound ratio of 3:4, 3:10 and 6:1, the lactic acid content decreased by 36.1%, 35.1% and 28.4% respectively (p<0.001), and the urea nitrogen content decreased by 16.1%, 13.8% and 15.1% respectively (p<0.05), indicating that when the compound ratio is 3:10~6:1, physical fatigue can be significantly relieved by reducing the body's lactic acid and urea nitrogen content. In addition, when the three ingredients are used in a compound ratio of 3:4, 3:10 and 6:1, the muscle glycogen content increased by 42.5%, 21.2% and 18.7% respectively (p<0.05), and the liver glycogen content increased by 32.2%, 13.7% and 17.8% respectively (p<0.05), indicating that when the compound ratio is 3:10~6:1, physical fatigue can be relieved by increasing the body's glycogen level.

[0084] In summary, when red ginseng powder + black wolfberry powder and ganoderma powder are compounded at a ratio of 3:10 to 6:1, the exhaustive swimming time of mice can be significantly prolonged by reducing the accumulation of metabolic products in the body of mice and increasing the body's glycogen level, indicating that this formula can relieve physical fatigue in mice, and the fatigue recovery effect is slightly higher than that of Red Bull. So far, the composition of the present invention has been proven to have a significant effect on relieving physical fatigue by the standard method of the "Health Food Function Test and Evaluation Method (2023 Edition)".

[0085] Comparative Example 1 The components in this scheme are: wolfberry fruit powder.

[0086] Comparative Example 2 The components in this solution are: sea buckthorn fruit powder.

[0087] Comparative Example 3 The components in this scheme are: Polygonatum odoratum powder.

[0088] Comparative Example 4 The components in this scheme are: 1 part of wolfberry fruit powder and 2 parts of sea buckthorn fruit powder.

[0089] Comparative Example 5 The components in this scheme are: 1 part of wolfberry fruit powder and 3 parts of polygonatum powder.

[0090] Comparative Example 6 The components in this scheme are: 2 parts of seabuckthorn fruit powder and 3 parts of polygonatum powder.

[0091] Comparative Example 7 The components in this solution are: 1 part of wolfberry fruit powder, 2 parts of seabuckthorn fruit powder and 3 parts of polygonatum powder.

[0092] The above comparative example was subjected to a zebrafish experiment according to the method of the above experimental example, and the test results are as follows: Table 6 Effect of comparison ratio on zebrafish fatigue

[0093] From the results in the above table, it can be seen that although wolfberry fruit powder, sea buckthorn fruit powder and polygonatum sibiricum powder each have the potential to relieve physical fatigue, when they are combined, whether in pairs or in combinations of three, the recovery rate does not change much, and the corresponding CI values ​​are 1.78, 1.69, 1.43 and 1.55, respectively, all greater than 1, proving that the various combinations of these three ingredients do not have a synergistic effect, and also indicating that food-borne ingredients that have the effect of relieving physical fatigue alone do not necessarily have a synergistic effect when combined with each other.

[0094] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition, characterized in that: The components of the composition are selected from at least two of red ginseng, black wolfberry and ganoderma lucidum.

2. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 2 to 8 parts of red ginseng and 1 to 9 parts of black wolfberry.

3. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 2 to 8 parts of red ginseng and 3 to 10 parts of ganoderma lucidum.

4. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 1 to 9 parts of black wolfberry and 3 to 10 parts of ganoderma lucidum.

5. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 2-8 parts of red ginseng, 1-9 parts of black wolfberry, and 3-10 parts of ganoderma lucidum.

6. The composition according to claim 5, characterized in that The composition consists of the following components and their weight parts by weight: 2 parts of red ginseng, 3 parts of black wolfberry, and 8 parts of ganoderma lucidum.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for relieving physical fatigue.

8. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for improving ATP energy metabolism or reducing lactic acid.

9. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for regulating the expression of Nrf2, atp2b1 and g6pca genes.

10. The use according to any one of claims 7 to 9, characterized in that: The types of the products include tablets, oral liquids, solid beverages, capsules, granules, powders, pills or tea bags.

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