Equivalent remineralized oral care composition, preparation method and oral care product

Abstract

The invention belongs to the technical field of oral care, and particularly relates to an equivalent remineralized oral care composition which comprises a dosage form A and a dosage form B which are independently stored, wherein the dosage form A comprises the following components in percentage by mass: 0.05-0.15% of fluoride, 0.1-1.5% of a biological adhesive, 40-70% of a humectant, 3-8% of a dispersing agent, 0.1-5% of a sweetening agent, 10-20% of a friction agent, 0.1-1% of a preservative, 1-3% of a foaming agent, 0.5-1.5% of essence, 0-1% of a pH regulator and a certain amount of water; the dosage form B comprises the following components in percentage by mass: 0.1-5% of calcium salt, 0.1-2% of zinc salt, 0.1-1.5% of a biological adhesive, 40-70% of a humectant, 3-8% of a dispersing agent, 0.1-5% of a sweetening agent, 10-20% of a friction agent, 0.1-1% of a preservative, 1-3% of a foaming agent, 0.5-1.5% of essence, 0-1% of a pH regulator and a certain amount of water.

Description

Technical Field

[0001] The invention belongs to the technical field of oral care, and in particular relates to an equivalent remineralization oral care composition and a preparation method thereof, and an oral care product. Background Art

[0002] Tooth enamel is the hardest tissue in the human body. Mature tooth enamel contains about 96% hydroxyapatite (HAP), 3% water, and 1% protein and lipid by weight. As the outermost layer of the tooth, tooth enamel is subject to various physical and chemical attacks, such as biting (up to 700N), friction, wear, and acid erosion from diet and bacteria.

[0003] The outermost layer of tooth enamel is in close contact with saliva and plaque fluid, and the HAP on its surface is in a dynamic balance of dissolution and redeposition. Oral cariogenic bacteria decompose food residues on the surface of teeth to produce acid, causing the demineralization and loss of hard tissues such as tooth enamel, dentin and cementum, which in turn leads to the formation of a series of oral diseases such as caries, tooth sensitivity, gingivitis, and gingivitis.

[0004] Fluoride is the most widely known active ingredient for preventing tooth decay. Fluoride can significantly reduce the dissolution rate of tooth minerals and promote the remineralization of demineralized teeth, thereby effectively preventing the occurrence of tooth decay. However, excessive intake of fluoride can pose a serious threat to human health, especially children's oral care products. According to GB / T 8372, the maximum amount of fluoride added to children's toothpaste is 1100ppm.

[0005] Therefore, how to avoid excessive fluoride intake without reducing the remineralization effect is a technical problem that urgently needs to be solved in this field.

[0006] It should be noted that the above information disclosed in this background technology section is only used to understand the background technology of the present application concept, and therefore, the above description is not considered to constitute information of the prior art. Summary of the invention

[0007] The embodiments of the present disclosure at least provide an equivalent re-mineralization composition and a preparation method thereof, and an oral care product.

[0008] In a first aspect, the embodiments of the present disclosure provide an equivalent mineralized oral care composition, comprising a formulation A and a formulation B which are stored separately; wherein the formulation A comprises the following components by weight: 0.05-0.15% fluoride, 0.1-1.5% bioadhesive, 40-70% humectant, 3-8% dispersant, 0.1-5% sweetener, 10-20% abrasive, 0.1-1% preservative, 1-3% foaming agent, 0.5-1.5% flavor, 0-1% pH adjuster 、 Some water;

[0009] The dosage form B comprises the following components by weight: 0.1-5% calcium salt, 0.1-2% zinc salt, 0.1-1.5% bioadhesive, 40-70% moisturizer, 3-8% dispersant, 0.1-5% sweetener, 10-20% abrasive, 0.1-1% preservative, 1-3% foaming agent, 0.5-1.5% flavor, 0-1% pH adjuster 、 Some water.

[0010] In an optional embodiment, the dosage form A and dosage form B are mixed in a ratio of 0.8:1-1.2:1.

[0011] In an optional embodiment, the fluoride refers to one or more common fluorides such as sodium fluoride, sodium monofluorophosphate, stannous fluoride, olafluor, amine fluoride, etc.

[0012] In an alternative embodiment, the bioadhesive agent is an acrylate copolymer.

[0013] In an optional embodiment, the calcium salt is one or more of calcium glycerophosphate, calcium lactate, calcium phosphate, calcium silicate, and the like.

[0014] In an optional embodiment, the pH values ​​of the dosage form A and the dosage form B are both 5.5-7.0.

[0015] In a second aspect, the disclosed embodiment also provides a method for preparing an equivalent remineralized oral care composition, comprising the following steps: Preparation steps of dosage form A: Step S1: Add water and moisturizer to a reactor, and stir and mix them evenly at 200-500 rpm; Step S2: Use a dispersant to disperse the bioadhesive into a uniform body and then add it to the reactor, and stir and mix it evenly; Step S3: Add a sweetener, a fluoride, a preservative, and a pH regulator to the reactor in sequence, stir and mix them evenly to ensure dissolution; Step S4: Add an abrasive to a reaction pot, stir and mix them evenly, and complete the paste making process; Step S5: Add a foaming agent and a flavor to the reactor, and stir and mix them evenly; Step S6: Discharge, test, and fill the mixture in the reactor in sequence to obtain dosage form A;

[0016] Or, preparation steps of dosage form B: step S1: add water and moisturizing agent to the reactor, and stir and mix evenly at 200-500rpm; step S2: use a dispersant to disperse the bioadhesive into a uniform body and then add it to the reactor, and stir and mix evenly; step S3: add sweetener, calcium salt, zinc salt, preservative, and pH regulator to the reactor in sequence, stir and mix evenly to ensure dissolution; step S4: add the friction agent to the reaction pot, stir and mix evenly, and complete the paste making process; step S5: add the foaming agent and flavor to the reactor, and mix and stir evenly; step S6: discharge, test, and fill the mixture in the reactor in sequence to obtain dosage form B.

[0017] In a third aspect, the embodiments of the present disclosure further provide an oral care product, wherein the packaging tube of the oral care product comprises: two independent chambers for respectively storing the dosage form A and the dosage form B in the oral care composition according to any one of claims 1 to 6.

[0018] In an optional embodiment, the oral care product is toothpaste.

[0019] The beneficial effect of the present invention is that by adding bioadhesive and zinc salt to formulation A and formulation B, the combined use of formulation A and formulation B can improve the utilization rate of fluoride and reduce fluoride intake by 50% without reducing the remineralization effect.

[0020] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention are realized and obtained by the structures particularly pointed out in the description and claims.

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below for detailed description as follows. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] The terms used herein are only used to describe specific exemplary configurations and are not intended to be limited. As used herein, the singular articles "one", "an" and "the" may also be intended to include plural forms, unless it is clearly indicated above that this is not the case. The terms "comprise", "include" and "have" are inclusive, and therefore specify the presence of features, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, elements, components and / or combinations thereof. The method steps, processes and operations described herein should not be interpreted as necessarily requiring them to be performed in the specific order discussed or shown, unless specifically identified as an execution order. Additional or alternative steps may be adopted.

[0024] As used herein, the phrases "in one embodiment," "according to one embodiment," "in some embodiments," and the like generally refer to the fact that the particular feature, structure, or characteristic following the phrase may be included in at least one embodiment of the present disclosure. Therefore, a particular feature, structure, or characteristic may be included in more than one embodiment of the present disclosure, so that these phrases do not necessarily refer to the same embodiment. As used herein, the terms "example," "exemplary," and the like are used to "serve as an example, instance, or illustration." Any implementation, aspect, or design described herein as "example" or "exemplary" is not necessarily to be construed as preferred or superior to other implementations, aspects, or designs. On the contrary, the use of the terms "example," "exemplary," and the like is intended to present concepts in a concrete manner.

[0025] Research has found that the disadvantages of the existing technology are: fluoride can significantly reduce the dissolution rate of tooth minerals and promote the remineralization of demineralized teeth, thereby effectively preventing the occurrence of dental caries. However, excessive intake of fluoride will pose a serious threat to human health, especially children's oral care products. According to GB / T 8372, the maximum amount of fluoride added to children's toothpaste is 1100ppm.

[0026] Based on the above research, the embodiments of the present disclosure provide an equivalent mineralized oral care composition to solve the above problems.

[0027] The defects existing in the above solutions are the results obtained by the inventor after practice and careful research. Therefore, the discovery process of the above problems and the solutions proposed by the present disclosure for the above problems below should be the contributions made by the inventor to the present disclosure during the disclosure process.

[0028] Some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0029] Example 1: Preparation of dosage form A. The component parts of Examples A1-A4 are shown in Table 1 below.

[0030] Table 1:

[0031]

[0032] Referring to Table 1, the main difference between Examples A1-A4 is the different fluoride contents. The fluoride component contents in Examples A1-A4 are respectively 0.11% sodium fluoride (fluorine content 500 ppm), 0.22% sodium fluoride (fluorine content 1000 ppm), 0.33% sodium fluoride (fluorine content 1500 ppm), and 0.66% sodium fluoride (fluorine content 3000 ppm); the contents of other components do not affect the test results.

[0033] Preparation method of dosage form A:

[0034] Step S1: adding water and moisturizing agent into a reactor, and stirring and mixing at 200-500 rpm;

[0035] Step S2: using a dispersant to disperse the bioadhesive into a uniform body, adding the body to the reactor, and stirring to mix evenly;

[0036] Step S3: adding the sweetener, fluoride, preservative, and pH regulator into the reactor in sequence, stirring and mixing them evenly to ensure that they are dissolved;

[0037] Step S4: adding the friction agent into the reaction pot, stirring and mixing evenly, and completing the paste making process;

[0038] Step S5: adding the foaming agent and the flavor into the reactor, and mixing and stirring them evenly;

[0039] Step S6: discharging, testing, filling and packaging the mixture in the reactor in sequence.

[0040] Hardness test method:

[0041] Select in vitro bovine tooth enamel blocks that are close to human enamel, cut the bovine enamel into 5mm*5mm*2mm enamel blocks, and finally prepare them into 1cm*1cm*1cm enamel blocks after resin embedding, inlaying, polishing, etc. Use a hardness tester to test the initial hardness of the enamel HV0, put the enamel block into a citric acid solution to simulate the enamel acid etching process, and use a hardness tester to test the hardness after acid etching HV1.

[0042] Sample test: toothpaste and water were diluted at a ratio of 1:1.6 (mass ratio) to prepare toothpaste slurry, which was then poured into a customized device containing enamel. Under certain load conditions, an electric toothbrush was used to brush the surface of the enamel block for 2 minutes to complete one test. The enamel treated with the sample to be tested was then placed in simulated saliva and stored in a constant temperature incubator at 37°C.

[0043] Repeat the above test steps 14 times (in vitro simulation of 7 days of use), the enamel hardness measured for the 14th time is HV 14 .

[0044] After 14 times of use, the change in enamel hardness value (△HV) is calculated as follows: △HV = HV 14 —HV1

[0045] The results of the enamel hardness test are shown in Table 1.1 below:

[0046] Table 1.1:

[0047] Hardness change value A1 A2 A3 A4 Fluorine content of product / ppm 500 1000 1500 3000 Fluorine content in use / ppm 500 1000 1500 3000 <h2 style=";text-align:left;direction:ltr"><![CDATA[△HV / kg·cm <h2 style=";text-align:left;direction:ltr"> 2 <h2 style=";text-align:left;direction:ltr"> :7d]]><h2 style=";text-align:left;direction:ltr"> 42 75 96 100

[0048] See Table 1.1. Through the remineralization study of different fluoride contents, after 14 treatments of the test samples (in vitro simulation of 1 week of use), the remineralization effect of fluoride increases with the increase of fluoride content. For example, after 7 days of treatment (14 product uses) of A1 with a fluoride content of 500ppm, the hardness of tooth enamel increased by 42kg·cm 2 However, as the fluoride content increases, the remineralization capacity of fluoride gradually slows down. However, considering the risk of fluoride intake, according to the GB / T 8372 toothpaste implementation standard, the maximum amount of fluoride added in toothpaste is 1500ppm (0.15%, calculated as fluoride); according to GB / T 8372, the maximum amount of fluoride added to children's toothpaste is 1100ppm (0.11%, calculated as fluoride). Therefore, it can be seen from the above that the amount of fluoride added is limited, and the corresponding remineralization capacity will also be weakened.

[0049] Example 2: Preparation of Formulation B. The component parts of Examples B1-B2 are shown in Table 2 below.

[0050] Table 2:

[0051]

[0052]

[0053] Referring to Table 2, the main difference between Examples B1 and B2 is the different calcium salt contents. The calcium salt component content in Example B1 is 1%; the calcium salt component content in Example B2 is 2%; the contents of other components do not affect the test results.

[0054] Preparation method of dosage form B:

[0055] Step S1: adding water and moisturizing agent into a reactor, and stirring and mixing at 200-500 rpm;

[0056] Step S2: using a dispersant to disperse the bioadhesive into a uniform body, adding the body to the reactor, and stirring to mix evenly;

[0057] Step S3: adding the sweetener, calcium salt, zinc salt, preservative, and pH regulator into the reactor in sequence, stirring and mixing evenly to ensure dissolution;

[0058] Step S4: adding the friction agent into the reaction pot, stirring and mixing evenly, and completing the paste making process;

[0059] Step S5: adding the foaming agent and the flavor into the reactor, and mixing and stirring them evenly;

[0060] Step S6: discharging, testing, filling and packaging the mixture in the reactor in sequence.

[0061] Using the same hardness test method as in Example 1, the enamel hardness test results are shown in Table 2.1 below.

[0062] Table 2.1:

[0063] Hardness change value B1 B2 Fluorine content of product / ppm 0 0 Fluorine content in use / ppm 0 0 <h2 style=";text-align:left;direction:ltr"><![CDATA[△HV / kg·cm <h2 style=";text-align:left;direction:ltr"> 2 <h2 style=";text-align:left;direction:ltr"> :7d]]><h2 style=";text-align:left;direction:ltr"> 56 57

[0064] As shown in Table 2.1, when calcium salt is added to the formula, it is helpful for the remineralization of tooth enamel, and its remineralization effect is better than that of Example A1 (fluorine content 500ppm) and worse than that of Example A2 (fluorine content 1000ppm). When the calcium salt addition amount is 1% (Example B1) or 2% (Example B2), there is no significant difference in the remineralization effect of tooth enamel, indicating that when the calcium salt addition amount is ≥1%, the remineralization capacity reaches the maximum, and its mineralization effect does not increase with the increase of calcium salt content, which means that using calcium salt as a way of remineralizing tooth enamel has certain limitations.

[0065] Comparative Example 1: Verification of the coexistence of calcium salt and fluoride in the same system. The number of components in Comparative Example 1 is shown in Table 3 below.

[0066] Table 3:

[0067]

[0068] Stability test:

[0069] After standing for 24 hours, the samples were placed in 100 ml transparent plastic bottles and left at 4°C, room temperature (20-25°C) and 45°C for 1 month to test the fluoride content. The test method is: GB / T8372-2017 Toothpaste.

[0070] The fluorine content test results are shown in Table 3.1 below.

[0071]

[0072] Referring to Table 3.1, by comparing Comparative Example C1 with Example A3, it is known that the free fluorine and total fluorine contents of C1, in which fluoride and calcium salt coexist in the same system, are significantly reduced at 4°C, room temperature (20-25°C), and 45°C. Compared with A3 containing the same amount of fluoride, the reduction in fluoride is significantly different, which, as a remineralization component, further affects the remineralization effect. Therefore, when fluoride and calcium salt coexist in the same system, free fluorine and free calcium combine to form more stable, insoluble calcium fluoride, which will lead to the inactivation of both active substances.

[0073] Example 3: Formulation A and Formulation B are mixed for use, and the mixed components are shown in Table 4 below:

[0074] Table 4:

[0075]

[0076] How to use the two-formula combination: When brushing your teeth, mix Formulation A and Formulation B in a 1:1 ratio, brush your teeth for 2 minutes, and then rinse your mouth with water.

[0077] The results of the enamel hardness test are shown in Table 4.1 below.

[0078] Table 4.1:

[0079]

[0080] See Table 4.1, the hardness improvement effect of "Combination 1" consisting of A1 and B1 is 45kg·cm 2 The hardness improvement effect of combination 1 is not significantly different from that of using A1 alone. However, combination 1 is a mixture of A1 and B1 in a ratio of 1:1, which means that the actual amount of fluoride in combination 1 is 250ppm during actual use. This means that the mixed use of A1 and B1 can reduce the amount of fluoride used and the amount of fluoride used by 50% without reducing the remineralization effect.

[0081] See Table 4.1, the hardness improvement effect of "Combination 2" consisting of A2 and B1 is 81kg·cm 2 The hardness improvement effect of combination 2 is not significantly different from that of A2 alone. However, combination 2 is a mixture of A2 and B1 in a ratio of 1:1, which means that the actual amount of fluoride in combination 2 is 500ppm. This means that the mixed use of A2 and B1 can reduce the amount of fluoride used and the amount of fluoride used by 50% without reducing the remineralization effect.

[0082] As shown in Table 2.1 and Table 4.1, the hardness improvement effect of "Combination 1" composed of A1 and B1 is only 80% of that of B1, and the hardness improvement effect of "Combination 2" composed of A2 and B1 is 1.42 times that of B2. When the actual amount of fluorine is low, that is, "Combination 1" is mainly calcium and fluorine is auxiliary to achieve the remineralization effect, so its hardness improvement value is weaker than B1. When the actual amount of fluorine is increased, that is, "Combination 2" is mainly fluorine and calcium is auxiliary to achieve the remineralization effect, and it can be seen from Table 2.1 that calcium salt remineralization has limitations, and the hardness improvement effect does not increase with the increase of its addition amount, so the hardness improvement value of combination 2 is 1.42 times that of B2, and the remineralization effect is significantly better than B2.

[0083] See Table 4.1, the hardness improvement effect of "Combination 3" consisting of A3 and B2 is 93kg·cm 2 The hardness improvement effect of combination 3 is not significantly different from that of A3 alone. However, combination 3 is a mixture of A3 and B2 in a ratio of 1:1, which means that the actual amount of fluoride in combination 3 is 750ppm during actual use. This shows that the mixed use of A3 and B2 can reduce the amount of fluoride used and the amount of fluoride used by 50% without reducing the remineralization effect.

[0084] See Table 4.1, the hardness improvement effect of "Combination 4" consisting of A4 and B2 is 105kg·cm 2 The hardness improvement effect of combination 4 is not significantly different from that of A4 alone. However, combination 4 is a mixture of A4 and B2 in a ratio of 1:1, which means that the actual amount of fluoride in combination 4 is 1500ppm. This shows that the mixed use of A4 and B2 can reduce the amount of fluoride used and the amount of fluoride used by 50% without reducing the remineralization effect.

[0085] Comparative Example 2: Anti-allergic effect of arginine in oral care products The number of components in Comparative Example 2 is shown in Table 5 below.

[0086] Table 5:

[0087]

[0088]

[0089] The results of the enamel hardness test are shown in Table 5.1 below:

[0090] Table 5.1:

[0091] Dosage Form Setting Bipolar Single dose Single dose Combination No. Combination 3 \ \ Recipe Number A3+B2 A3 D1 Fluorine content in use / ppm 750 1500 1500 <h2 style=";text-align:left;direction:ltr"><![CDATA[△HV / kg·cm <h2 style=";text-align:left;direction:ltr"> -2 <h2 style=";text-align:left;direction:ltr"> :7d]]><h2 style=";text-align:left;direction:ltr"> 93 96 102 Compared with A3 0.97 1 1.06

[0092] See Table 5.1. From the hardness test results, it can be seen that compared with A3, the double-dose mixed use (A3+B2) scheme has an effect of improving hardness of 0.97 times that of A3, which is close to the remineralization effect of A3. D1, which uses arginine to synergize with fluorine remineralization, has an effect of improving hardness of 1.06 times that of A3, that is, the addition of arginine has improved the fluoride remineralization effect to a certain extent, which is reflected in the increase of 6% in the hardness change value. However, compared with the fluorine content during use, it can be seen that the fluorine content in the A3 and D1 samples is twice that of the double-dose mixed use (A3+B2) scheme. In the range of hardness change values ​​from -3% to 6%, the double-dose mixed use (A3+B2) scheme minimizes the use of 50% fluorine. Therefore, compared with the arginine synergistic fluorine remineralization scheme, under the premise that the difference in hardness improvement value and remineralization effect is not significant, the double-dose mixed use (A3+B2) scheme is safer and can avoid the risk of excessive fluoride intake and swallowing.

[0093] Example 4: The components of the newly added comparative example A5 and example A2 are shown in Table 6 below.

[0094] Table 6:

[0095]

[0096]

[0097] Referring to Table 6, the difference between Example A2 and Comparative Example A5 is that a bioadhesive is added to Example A2. Comparative Example 3: The components of Comparative Example D4 and Comparative Example D3 are shown in Table 7 below.

[0098] Table 7:

[0099]

[0100]

[0101] Referring to Table 7, the difference between Comparative Example D3 and Example B1 is that a bioadhesive is added to Example B1; the difference between Comparative Example D4 and Example B2 is that a zinc salt is added to Example B2.

[0102] Bioadhesion testing:

[0103] Test method: Use an in vitro oral esophageal retention model to study the mucosal adhesion of the sample. Attach the mucosa to the slope, pour mouthwash from top to bottom to simulate the human mouthwashing process, and then pour simulated saliva to simulate the flushing process. Finally, collect the residue on the membrane, test the percentage of active content, and confirm the mucosal adhesion. The larger the residual percentage, the better the mucosal adhesion and the better the retention effect of the active substance.

[0104] Fluoride detection method: GB / T 8372-2017 Toothpaste.

[0105] Zinc detection method: QB / T 5406-2019 Detection of acid-soluble zinc in oral cleaning care products, toothpaste and mouthwash - atomic absorption spectrophotometry.

[0106] Calcium detection method: GB 5009.92-2016 National Food Safety Standard Determination of Calcium in Food.

[0107] Evaluation criteria:

[0108] When the retention on the membrane is above 15.00%, the mucosal adhesion function is very good;

[0109] When the retention amount on the membrane is above 10.00-14.99%, the mucosal adhesion function is very good;

[0110] When the amount retained on the membrane is above 5.00-9.99%, the mucosal adhesion function is good;

[0111] When the amount retained on the membrane is above 1.00-4.99%, the mucosal adhesion function is general;

[0112] When the retention amount on the membrane is above 0-0.99%, the mucosal adhesion function is poor.

[0113] The retention test of fluorine, zinc and calcium is shown in Table 8 below.

[0114] Table 8:

[0115]

[0116]

[0117] The results of the enamel hardness test are shown in Table 9 below.

[0118] Table 9:

[0119]

[0120] Referring to Tables 8 and 9, Combination 2 with bioadhesives has very good performance in terms of the retention of fluoride, calcium salt, and zinc salt, while Combination 5 composed of conventional formula systems A5 and D3 without this function has average or poor performance in terms of the retention of active substances. During the use of toothpaste, the foaming agent in the toothpaste will take away the active substances, resulting in a significant reduction in the utilization rate of the functional ingredients, thereby reducing the product efficacy, that is, the size of the retention of the functional ingredients directly affects the manifestation of the product efficacy. A2 and A5 both contain 1000ppm of fluoride, and B1 and D3 contain the same amount of calcium salt (1% calcium glycerophosphate). From the results of the enamel hardness test, it can be seen that the repair effect of Combination 5 composed of A5 and D3 on enamel is only 74.07% of that of Combination 2, that is, compared with the repair effect of Combination 5 on enamel hardness, Combination 2 with bioadhesive function can increase the enamel hardness by 35%. Therefore, the solution formed by the combination of A2 and B1 (Combination 2) has a better enamel repair effect, that is, under the premise of the same fluoride and calcium salt usage and the same mixing method, Combination 2 formed by A2 and B1 has the effect of significantly improving the hardness of enamel and repairing enamel.

[0121] Antibacterial test:

[0122] Reference method: WS / T 650-2019 Antibacterial and antibacterial effect evaluation method.

[0123] Method of action: According to the product usage instructions, dilute and test at a ratio of 1:1.6 (paste: deionized water, mass ratio).

[0124] Test bacteria: Streptococcus mutans (cariogenic bacteria).

[0125] Evaluation criteria: if the inhibition rate is ≥50% to 90%, it is judged to have an antibacterial effect; if the inhibition rate is ≥90%, it is judged to have a strong antibacterial effect.

[0126] The antibacterial test results are shown in Table 10 below.

[0127] Table 10:

[0128]

[0129] The results of the enamel hardness test are shown in Table 11 below.

[0130] Table 11:

[0131]

[0132] Hydroxyapatite, as the main component of teeth, has poor tolerance to acid. When in an acidic environment, the enamel of teeth dissolves and demineralizes, and the hardness of enamel decreases. When teeth are in acidic conditions for a long time, the rate of acid erosion and demineralization is always greater than the rate of remineralization. If this continues for a long time, it will lead to the formation of dental caries. Oral pathogens, such as the cariogenic Streptococcus mutans, can metabolize oral substrates (such as food residues) to produce acidic substances. Therefore, inhibiting the reproduction of oral pathogens and preventing their metabolism from producing acidic substances can indirectly protect teeth from the effects of acid erosion and demineralization, thereby allowing the enamel to maintain its original hardness.

[0133] Referring to Table 10 and Table 11, it can be seen from the test results that both combination 2 and combination 3 have a strong antibacterial effect, that is, they can inhibit the reproduction of oral pathogens, while combination 6, combination 7, and combination 8 do not have an antibacterial effect, that is, they cannot inhibit or prevent oral pathogens from metabolizing and producing acidic substances. From the enamel hardness test results, it can be seen that for combination 2, combination 6, and combination 7 with a fluorine content of 500ppm, compared with combination 2, the repair ability of combination 6 on enamel decreased by 14%, and combination 7 decreased by 38%. For combination 3 and combination 8 with a fluorine content of 750ppm, compared with combination 3, the repair ability of combination 8 on enamel decreased by 13%. Such test results are consistent with the antibacterial test results. Therefore, when the formula contains zinc salt, it can assist fluoride and calcium salt remineralization and enhance the remineralization effect. Therefore, combination 2 and combination 3 are a preferred solution.

[0134] By comparing Table 8 and Table 9 with Table 1, it can be seen that the actual fluoride content of combination 2 with the addition of bioadhesive is 500 ppm, and the change value of enamel hardness is 81 kg·cm 2 ; The fluoride content of single-dose A2 in Table 1 is 1000 ppm, and the change in enamel hardness is 75 kg·cm 2 Therefore, data comparison shows that the addition of bioadhesives can improve the utilization rate of fluoride, reduce fluoride intake by 50%, and significantly improve the remineralization effect. By comparing Table 10 and Table 11 with Table 1, it can be seen that the actual fluoride content of combination 2 and combination 3 with zinc salt added is 500ppm and 750ppm, respectively, and the change in enamel hardness is 81kg·cm 2 and 93kg·cm 2 The actual fluoride content of single-dose A2 in Table 1 is 1000 ppm, and the change in enamel hardness is 75 kg·cm 2 Therefore, the utilization of fluoride can be improved by adding zinc salts, and the fluoride intake is reduced by 50%, but the remineralization effect is improved.

[0135] In summary, the dosage forms A and B of the present invention are mixed and used as an equivalent remineralization composition, which can reduce the intake of fluoride by 50% without reducing the remineralization effect.

[0136] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A remineralizing oral care composition, characterized in that The invention comprises a dosage form A and a dosage form B which are stored separately; wherein The dosage form A comprises the following components in parts by weight: 0.05-0.15% fluoride, 0.1-1.5% bioadhesive, 40-70% humectant, 3-8% dispersant, 0.1-5% sweetener, 10-20% abrasive, 0.1-1% preservative, 1-3% foaming agent, 0.5-1.5% flavor, 0-1% pH adjuster, and some water; The dosage form B comprises the following components in parts by weight: 0.1-5% calcium salt, 0.1-2% zinc salt, 0.1-1.5% bioadhesive, 40-70% humectant, 3-8% dispersant, 0.1-5% sweetener, 10-20% abrasive, 0.1-1% preservative, 1-3% foaming agent, 0.5-1.5% flavor, 0-1% pH adjuster, and some water.

2. The oral care composition according to claim 1, characterized in that The mixing ratio of the dosage form A and the dosage form B is 1:

1.

3. The oral care composition according to claim 1, characterized in that The fluoride is one or more of sodium fluoride, sodium monofluorophosphate, stannous fluoride, olafluor, and amine fluoride.

4. The oral care composition according to claim 1, characterized in that The bioadhesive is an acrylic acid ester copolymer.

5. The oral care composition according to claim 4, characterized in that The calcium salt is one or more of calcium glycerophosphate, calcium lactate, calcium phosphate, and calcium silicate.

6. The oral care composition according to claim 1, characterized in that The pH values ​​of the formulation A and the formulation B are both 5.5-7.

0.

7. A method for preparing a remineralizing oral care composition according to any one of claims 1 to 6, characterized in that: The steps include: The preparation steps of dosage form A include: Step S1: adding water and moisturizing agent into a reactor, and stirring and mixing at 200-500 rpm; Step S2: using a dispersant to disperse the bioadhesive into a uniform body, adding the body to the reactor, and stirring to mix evenly; Step S3: adding the sweetener, fluoride, preservative, and pH regulator into the reactor in sequence and stirring and mixing them evenly to ensure that they are dissolved; Step S4: adding the friction agent into the reaction pot, stirring and mixing evenly, and completing the paste making process; Step S5: adding the foaming agent and the flavor into the reactor, and mixing and stirring them evenly; Step S6: discharging, testing, and filling the mixture in the reactor in sequence to obtain dosage form A; Alternatively, the preparation steps of dosage form B include: Step S1: adding water and moisturizing agent into a reactor, and stirring and mixing at 200-500 rpm; Step S2: using a dispersant to disperse the bioadhesive into a uniform body, adding the body to the reactor, and stirring to mix evenly; Step S3: adding the sweetener, calcium salt, zinc salt, preservative, and pH regulator into the reactor in sequence, stirring and mixing evenly to ensure dissolution; Step S4: adding the friction agent into the reaction pot, stirring and mixing evenly, and completing the paste making process; Step S5: adding the foaming agent and the flavor into the reactor, and mixing and stirring them evenly; Step S6: discharging, testing, and filling the mixture in the reactor in sequence to obtain dosage form B.

8. An oral care product, characterized in that: The packaging tube of the oral care product comprises: two independent chambers for respectively storing the dosage form A and the dosage form B in the oral care composition according to any one of claims 1 to 6.

9. The oral care product according to claim 8, characterized in that The oral care product is toothpaste.

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