A pesticide composition and its application

Abstract

The present invention belongs to the field of pesticide control technology and relates to a pesticide composition and its application. The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula (I) and active ingredient B is a neonicotinoid compound, with the mass ratio of active ingredient A to active ingredient B being 1:85 to 45:1. The pesticide composition of the present invention exhibits significant synergistic effects, rapid efficacy, a long-lasting effect, increased sites of action, a broadened control spectrum, and can reduce the field dosage of the active ingredient, thereby reducing production and usage, and helping to delay the development of pesticide resistance in pests and mites.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticide control, and in particular relates to a pesticide composition and application thereof. Background Art

[0002] Neonicotinoid insecticides, due to their high efficacy, broad spectrum, good root systemic activity, contact toxicity, and stomach toxicity, can be used for both stem and foliage treatments and soil and seed treatments, making them a relatively effective class of insecticides for controlling piercing-sucking pests and small lepidopteran and coleopteran pests. With the widespread and increasing frequency of neonicotinoid use, various targets have developed varying degrees of resistance to neonicotinoids.

[0003] In agricultural production, the control of various types of harmful insects mainly relies on the application of large quantities of pesticides and insecticides. The large-scale, frequent and irrational use of pesticides and insecticides has led to the continuous increase in the resistance of harmful insects, making prevention and control increasingly difficult and causing more serious damage to crops. By mixing the compound of formula (I) with a neonicotinoid compound agent, a synergistic effect of insecticide and acaricide is achieved at a certain mass ratio, which can treat different pests and mites, expand the control spectrum, improve the efficacy of the agent, and reduce the development of pest resistance. To date, there have been no reports on the use of compound (I) mixed with neonicotinoid insecticides to control pests and mites. Summary of the Invention

[0004] The purpose of the present invention is to provide a pesticide composition containing neonicotinoid compounds which has synergistic effects, reduces resistance and has low use cost.

[0005] To achieve the above object, the present invention adopts the following technical solution: a pesticide composition, wherein the active ingredients of the pesticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) The active ingredient B is a neonicotinoid compound;

[0006] Furthermore, the neonicotinoid compounds include flubendiamide, sulfoxaflor, clothianidin, thiamethoxam, acetamiprid, thiacloprid, imidacloprid, dinotefuran, nitenpyram, nicotine, flupyradone, trifluanid, nithiazine or chlorothiazide;

[0007] Furthermore, the neonicotinoid compound is thiamethoxam, acetamiprid, imidacloprid, and nitenpyram;

[0008] Furthermore, the mass ratio between the active ingredient A and the active ingredient B is 1:85 to 45:1 or any value between the above values;

[0009] Furthermore, the mass ratio between the active ingredient A and the active ingredient B is 1:50 to 40:1;

[0010] Furthermore, the neonicotinoid compound is thiamethoxam, and the mass ratio of the compound of formula (I) to thiamethoxam is 1:33 to 40:1.

[0011] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:33, 1:18, 1:16, 1:12, 1:6, 2:1, 4:1, 10:1, 16:1, 20:1, 35:1, 40:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:16 to 20:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to thiamethoxam is 1:16, 1:12, 1:6, 2:1, 4:1, 10:1, 16:1, 20:1;

[0014] Furthermore, the neonicotinoid compound is acetamiprid, and the mass ratio of the compound of formula (I) to acetamiprid is 1:30 to 37:1.

[0015] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30, 1:25, 1:20, 1:17, 1:15, 1:8, 2:15, 1:3, 3:1, 4:1, 8:1, 15:1, 20:1, and 37:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30 to 20:1,

[0017] Furthermore, the mass ratio of the compound of formula (I) to acetamiprid is 1:30, 1:25, 1:20, 1:17, 1:15, 1:8, 2:15, 1:3, 3:1, 4:1, 8:1, 15:1, 20:1;

[0018] Furthermore, the neonicotinoid compound is imidacloprid, and the mass ratio of the compound of formula (I) to imidacloprid is 1:50 to 30:1.

[0019] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50, 1:25, 1:20, 1:10, 1:7, 1:3, 1:1, 3:1, 7:1, 10:1, 17:1, 20:1, and 30:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50 to 20:1,

[0021] Furthermore, the mass ratio of the compound of formula (I) to imidacloprid is 1:50, 1:25, 1:20, 1:10, 1:7, 1:3, 1:1, 3:1, 7:1, 10:1, 17:1, and 20:1;

[0022] Furthermore, the neonicotinoid compound is nitenpyram, and the mass ratio of the compound of formula (I) to nitenpyram is 1:50 to 30:1;

[0023] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:50, 1:26, 1:13, 1:5, 1:2, 5:1, 10:1, 13:1, 20:1, 25:1, 30:1;

[0024] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:26 to 25:1;

[0025] Furthermore, the mass ratio of the compound of formula (I) to nitenpyram is 1:26, 1:13, 1:5, 1:2, 5:1, 10:1, 13:1, 20:1, and 25:1;

[0026] Furthermore, based on the total mass of the pesticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%;

[0027] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1% to 85%;

[0028] Furthermore, the pesticide composition may include, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist, or a carrier;

[0029] Furthermore, the pesticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0030] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0031] Furthermore, the dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0032] Furthermore, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0033] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0034] Furthermore, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0035] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0036] Furthermore, the defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0037] Furthermore, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0038] Furthermore, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0039] Furthermore, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0040] Furthermore, the synergist is selected from synergist phosphine and piperonyl butoxide; and / or

[0041] Furthermore, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0042] Furthermore, the pesticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0043] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0044] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0045] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0046] Application of the pesticide composition of the present invention in preventing and controlling agricultural, forestry and sanitary pests and mites;

[0047] Furthermore, the agricultural and forestry pests and mites are pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0048] Furthermore, the pests include aphids and whiteflies;

[0049] Furthermore, the harmful mites include Panonychus citri, Tetranychus cinnabarinus, Tetranychus urticae, and Tetranychus truncatus.

[0050] The present invention also provides a method for using the neonicotinoid-containing pesticide composition as described above, specifically applying an effective dose to the pests to be controlled or the medium in which they grow.

[0051] The pesticide composition of the present invention has the following advantages:

[0052] 1) The pesticide composition of the present invention has obvious synergistic effect, rapid efficacy, long lasting effect, more sites of action, and a wide spectrum of control;

[0053] 2) The pesticide composition of the present invention has a significant effect and can reduce the amount of active ingredients used in the field, thereby reducing production and usage components, which is beneficial to delaying the development of pesticide resistance in pests and mites.

[0054] 3) The pesticide composition of the present invention is environmentally friendly and has low toxicity to humans and animals. DETAILED DESCRIPTION

[0055] The present invention is further described below with reference to the examples. The percentages in the examples are all by weight, but the present invention is not limited thereto.

[0056] The composition of the present invention can be provided in the form of a formulation. It can be formulated as a suspension concentrate, water-dispersible granules, aqueous emulsion, granules, wettable powder, or dispersible oil suspension, as needed. The content of the active ingredient in the composition of the present invention depends on the dosage when used alone, as well as the blending ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0057] Preparation Example

[0058] Example 1:

[0059] 42% formula (Ⅰ) compound·thiamethoxam wettable powder (6:36)

[0060] Formula: 6% compound of formula (I), 36% thiamethoxam, 3% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8% tea saponin, 2% sodium lauryl sulfate, 2% citric acid, and kaolin to make up the balance;

[0061] Preparation method: Active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and pulverized and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0062] Example 2:

[0063] 40% formula (Ⅰ) compound acetamiprid wettable powder (4:36)

[0064] Formula: 4% compound of formula (I), 36% acetamiprid, 3% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8.5% tea saponin, 2.5% sodium lauryl sulfate, and kaolin to make up the balance;

[0065] Preparation method: same as Example 1.

[0066] Example 3:

[0067] 42% formula (Ⅰ) compound·nitenpyram wettable powder (7:35)

[0068] Formula: 7% compound of formula (I), 35% nitenpyram, 5% naphthalenesulfonate formaldehyde condensate, 1.5% polyoxyethylene ether nonionic (NV1203), 8% tea saponin, 2% sodium lauryl sulfate, and kaolin to make up the balance;

[0069] Preparation method: same as Example 1.

[0070] Example 4:

[0071] 27% formula (Ⅰ) compound·imidacloprid suspension concentrate (3:24)

[0072] Formula: 3% compound of formula (I), 24% imidacloprid, 1.5% sulfonate formaldehyde condensate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% styrenated phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 1% sodium sorbate, 4.5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0073] Preparation method: Add wetting dispersant, defoamer and other ingredients to water and stir evenly, then add active ingredients, use zirconium oxide beads, and use a sand mill to wet grind to D 90 (90% of the particles have a particle size of less than 5 μm) to obtain a crushed slurry. A thickener, antifreeze agent, and preservative are added to the crushed slurry and mixed evenly. Deionized water is added to 100% and the suspension product is obtained by high-speed shearing.

[0074] Example 5:

[0075] 64% formula (Ⅰ) compound·thiamethoxam water dispersible granules (8:56)

[0076] Formula: 8% compound of formula (I), 56% thiamethoxam, 6% polycarboxylate sodium salt, 2% dispersant NNO, 6% naphthalenesulfonate formaldehyde condensate, 2.5% polycarboxylate anion (WG5), 2% sodium lauryl sulfate, 2% citric acid, 4% sodium sulfate, and kaolin to make up the balance;

[0077] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, appropriate water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0078] Example 6:

[0079] 56% formula (Ⅰ) compound acetamiprid water dispersible granules (7:49)

[0080] Formula: 7% compound of formula (I), 49% acetamiprid, 2% sodium lignin sulfonate, 3.5% sodium salt of polycarboxylate, 8% naphthalenesulfonate formaldehyde condensate, 2.5% polycarboxylate anion (WG5), 2% sodium lauryl sulfate, 4.5% white carbon black, and kaolin makes up the balance;

[0081] Preparation method: same as Example 5.

[0082] Example 7:

[0083] 48% formula (Ⅰ) compound·imidacloprid water dispersible granules (6:42)

[0084] Formula: 6% compound of formula (I), 42% imidacloprid, 6% sodium salt of polycarboxylate, 7.5% naphthalenesulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 2.5% polycarboxylate anion (WG5), 4% starch, and kaolin to make up the balance;

[0085] Preparation method: same as Example 5.

[0086] Example 8:

[0087] 60% formula (Ⅰ) compound·nitenpyram water dispersible granules (10:50)

[0088] Formula: 10% compound of formula (I), 50% nitenpyram, 6% sodium salt of polycarboxylate, 8% naphthalenesulfonate formaldehyde condensate, 2% sodium lauryl sulfate, 2.5% polycarboxylate anion (WG5), 4% sodium sulfate, and kaolin to make up the balance;

[0089] Preparation method: same as Example 5.

[0090] Indoor biological activity assay

[0091] Examples refer to the Guidelines for Indoor Pesticide Bioassays, Part 6: Insect Dip Method NY / T 1154.6-2006; Part 7: Determination of Combined Effects of Mixtures NY / T 1154.7-2006; and Part 12: Slide Dip Method for Spider Mites NY / T 1154.12-2008.

[0092] Specific indoor toxicity test:

[0093] 1) Test target: Panonychus citri

[0094] Test method: spider mite slide dipping method;

[0095] Test source: Panonychus citri; select nymphs of the mites, reared indoors and in consistent physiological condition. Cut double-sided tape into 2 cm lengths and attach to one end of a glass slide. Then, select healthy mites and attach their backs to the tape. Place 30 mites per slide in a container lined with a damp sponge, cover, and store at (25 ± 1)°C. After 2 hours, examine under a microscope, remove dead and injured individuals, and replenish the remaining mites to 25 per slide.

[0096] Preparation of the reagent: The test reagent was prepared into a mother solution using an organic solvent (acetone), and then 5 series of mass concentrations were prepared using a 0.1% Tween 80 aqueous solution according to the method of equal proportions.

[0097] Experimental treatment: Immerse the glass slide in the drug solution and shake it gently for 5 seconds, then take it out and absorb the excess drug solution with absorbent paper. Place it on a white porcelain plate with a wet sponge, cover it with a plastic film with good light transmittance, and place it at (25±1)℃ for observation.

[0098] Experimental repetition: Each treatment was repeated 4 times, and a treatment without drug was set as a blank control.

[0099] Investigation time: 24 hours after treatment, check the death of test insects, record the total number of mites and the number of dead mites, and calculate the mortality rate.

[0100] 2) Test target: aphids

[0101] Test method: insect immersion method;

[0102] Test insects: healthy, uniformly grown, 2nd-instar aphids;

[0103] Preparation of drugs: The test drugs were dissolved in acetone and prepared into 5 series of concentrations using 0.1% Tween 80 aqueous solution.

[0104] Experimental treatment: Select 2nd instar larvae raised indoors and in the same physiological state. Use an insect immersion device to immerse the test insects in the drug solution for 5 seconds. Each treatment is 15 larvae, repeated 4 times. The control group is treated with 0.1% Tween 80 aqueous solution. After treatment, the insects are transferred to a culture dish with a diameter of 9.0 cm covered with filter paper for breeding, and fresh leaves are replaced every day.

[0105] The treated test insects were placed in an artificial intelligence culture room at 25±1°C, a light period of L:D=14h:10h, and a relative humidity of 65%±5% for breeding.

[0106] Investigation method: After the treatment with the pesticide, the death of the test insects was investigated 24 hours later. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The total number of insects and the number of dead insects were recorded respectively.

[0107] Data statistics and analysis:

[0108] Based on the survey data, calculate the adjusted mortality rate of each treatment using the following formula, with the results rounded to two decimal places:

[0109]

[0110] Where:

[0111] P——mortality rate, in percentage (%);

[0112] K——indicates the number of dead insects, the unit is head;

[0113] N——represents the total number of insects processed, in heads.

[0114]

[0115] Where:

[0116] P1——adjusted mortality rate, in percentage (%);

[0117] P t ——Treatment mortality rate, expressed in percentage (%);

[0118] P0 - blank control mortality rate, in percentage (%).

[0119] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0120] The data were processed by probability value analysis. The DPS statistical analysis system was used to analyze the toxicity regression line and LC 50 The activity of the test agents on the biological materials was evaluated by using the values, 95% confidence limits and correlation coefficient r.

[0121] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0122]

[0123] Where:

[0124] ATI - measured toxicity index of mixture;

[0125] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0126] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0127] TTI=TI A *P A +TIB *P B

[0128] Where:

[0129] TTI – Theoretical Toxicity Index of Mixtures;

[0130] TI A ——Agent toxicity index;

[0131] P A ——The percentage of agent A in the mixture, in percentage (%);

[0132] TI B ——Toxicity index of agent B;

[0133] P B ——The percentage of agent B in the mixture, in percentage (%).

[0134]

[0135] Where:

[0136] CTC – Co-toxicity coefficient;

[0137] ATI - measured toxicity index of mixture;

[0138] TTI - Theoretical Toxicity Index of Mixture.

[0139] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0140] Results of the combined toxicity test of Panonychus citri:

[0141] LC of the compound of formula (I) against Panonychus citri 50 The concentration of the compound of formula (I) is 3.156 mg / L. The mixture of the compound of formula (I) with thiamethoxam, acetamiprid, imidacloprid and nitenpyram has a good synergistic effect on Panonychus citri.

[0142] As shown in Table 1, when the mass ratio of the compound of formula (I) to thiamethoxam is 1:63 to 35:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:33 to 35:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on citrus mites.

[0143] Table 1 Toxicity test results of different ratios of compound of formula (I) and thiamethoxam to Panonychus citri

[0144]

[0145] As shown in Table 2, when the mass ratio of the compound of formula (I) to acetamiprid is 1:30-40:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus Panonychus citri is additive or synergistic. Among them, when the mass ratio is 1:30-15:1, the co-toxicity coefficient is greater than 120, which shows a good synergistic effect on citrus Panonychus citri.

[0146] Table 2 Toxicity test results of different ratios of compound of formula (I) and acetamiprid to Panonychus citri

[0147]

[0148] As shown in Table 3, when the mass ratio of the compound of formula (I) to imidacloprid is 1:85-30:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus mites shows an additive or synergistic effect. Among them, when the mass ratio is 1:50-30:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on citrus mites.

[0149] Table 3 Toxicity test results of different ratios of compound of formula (I) and imidacloprid against Panonychus citri

[0150]

[0151] As shown in Table 4, when the mass ratio of the compound of formula (I) to nitenpyram is 1:50-45:1, the co-toxicity coefficient is greater than 80, and the control effect on citrus Panonychus citri is additive or synergistic. Among them, when the mass ratio is 1:50-20:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on citrus Panonychus citri.

[0152] Table 4 Toxicity test results of different ratios of compound of formula (I) and nitenpyram to Panonychus citri

[0153]

[0154] Results of the combined toxicity test of the mixed pair against aphids:

[0155] LC of the compound of formula (I) against aphids 50 The LC values ​​of thiamethoxam, acetamiprid, imidacloprid and nitenpyram against aphids are 127.517 mg / L. 50 The concentrations of the compounds of formula (I) were 5.293 mg / L, 2.338 mg / L, 24.715 mg / L and 3.935 mg / L respectively. The mixture of the compound of formula (I) with thiamethoxam, acetamiprid, imidacloprid and nitenpyram had a good combined effect on aphids.

[0156] As shown in Table 5, when the mass ratio of the compound of formula (I) to thiamethoxam was 1:18 to 40:1, the co-toxicity coefficient was greater than 120, showing a good synergistic effect on the control of aphids.

[0157] Table 5 Toxicity test results of different ratios of compound of formula (I) and thiamethoxam to aphids

[0158]

[0159] As shown in Table 6, when the mass ratio of the compound of formula (I) to acetamiprid is 1:25 to 37:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on the control of aphids. Among them, when the mass ratio of the compound of formula (I) to acetamiprid is 1:8 to 20:1, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect.

[0160] Table 6 Toxicity test results of different ratios of the compound of formula (I) and acetamiprid to aphids

[0161]

[0162] As shown in Table 7, when the mass ratio of the compound of formula (I) to imidacloprid is 1:25 to 17:1, the co-toxicity coefficient is greater than 120, showing a good synergistic effect on the control of aphids. Among them, when the mass ratio of the compound of formula (I) to imidacloprid is 1:25 to 7:1, the co-toxicity coefficient is greater than 130, showing a significant synergistic effect.

[0163] Table 7 Toxicity test results of different ratios of compound of formula (I) and imidacloprid to aphids

[0164]

[0165] As shown in Table 8, when the mass ratio of the compound of formula (I) to nitenpyram was 1:26-30:1, the co-toxicity coefficient was greater than 140, showing a significant synergistic effect on the control of aphids.

[0166] Table 8 Toxicity test results of different ratios of compound of formula (I) and nitenpyram to aphids

[0167]

[0168]

[0169] Field efficacy examples

[0170] Test crops and targets: Citrus navel orange, citrus spider mite, citrus aphid;

[0171] Experimental location: Citrus orchard in Changyang County, Yichang City;

[0172] Experimental design: Each group of experiments consisted of 6 citrus trees (fixed and marked), with 3 replicates and random arrangement.

[0173] Test time: The test application time for controlling citrus red spider mites is May 2, 2023, and the test application time for controlling citrus aphids is August 29, 2023.

[0174] Spraying equipment: 3WBS-16 knapsack electric sprayer. First spray the water control plot, then the test agent plot. On a clear day, spray evenly throughout the tree, wetting both sides of the leaves until water drips. Use 1.8L of spray per plant.

[0175] Experimental environment: The cultivation conditions in all experimental plots were uniform and consistent, and the same fertilizer and water management levels were adopted. The citrus fruits grew well, and no agricultural operations such as fertilization, irrigation and drainage were carried out during the experiment.

[0176] Test agent:

[0177] Table 9 Field test pesticides and dosage

[0178]

[0179] Survey Method: Tree and leaf surveys were conducted before, 1 day after, and 7 days after each spraying. Three leaves were selected from each citrus tree in the east, south, west, and north directions. These leaves were labeled and the number of live insects on both the front and back sides was counted. The control efficacy was calculated and analyzed for significance.

[0180] Calculation formula and data analysis:

[0181]

[0182]

[0183] Field efficacy test results:

[0184] The test results show that the control effects of different pesticides on citrus red spider mites are shown in Table 10. It can be seen from the table that 1 day after the drug application, 64% of the formula (I) compound·thiamethoxam water dispersible granules (8:56), 56% of the formula (I) compound·acetamiprid water dispersible granules (7:49), 48% of the formula (I) compound·imidacloprid water dispersible granules (6:42), and 60% of the formula (I) compound·nitenpyram water dispersible granules (10:50) showed good fast-acting properties, with a control effect of more than 80%; after the drug application, On the 7th day, the protective efficacy of 64% formula (I) compound·thiamethoxam water-dispersible granules (8:56), 56% formula (I) compound·acetamiprid water-dispersible granules (7:49), 48% formula (I) compound·imidacloprid water-dispersible granules (6:42), and 60% formula (I) compound·nitram water-dispersible granules (10:50) was improved to 91.86%, 91.64%, 90.81%, and 91.79%, respectively. Each mixed preparation also showed good persistence, with a protective efficacy of more than 90%.

[0185] 1d and 7d after application, there was no significant difference in the control effect of 64% formula (I) compound·thiamethoxam water dispersible granules (8:56), 56% formula (I) compound·acetamiprid water dispersible granules (7:49), 48% formula (I) compound·imidacloprid water dispersible granules (6:42), and 60% formula (I) compound·nitram water dispersible granules (10:50) on citrus red spider mites, but there was a significant difference between them and the other treatments, which was significantly higher than the control single agent and mixed preparation (20% imidacloprid·triazotin wettable powder).

[0186] Table 10 Control effects of different pesticides on citrus red spider mites

[0187]

[0188]

[0189] Note: The above data are the average of 3 repetitions, with two decimal places, and the difference in efficacy is at the 0.05% level.

[0190] The test results show that the control effects of different pesticides on aphids are shown in Table 11. It can be seen from the table that the insect population base survey before application found that the insect population base of the experimental treatments H-1, H-2, H-3, and H-4 aphids was less than that of other experimental treatments, proving that the first application of pesticides to control citrus mites can also effectively reduce the occurrence of citrus aphids in the later period.

[0191] In addition, 1 day after the application, 64% of the compound of formula (I)·thiamethoxam water dispersible granules (8:56), 56% of the compound of formula (I)·acetamiprid water dispersible granules (7:49), 48% of the compound of formula (I)·imidacloprid water dispersible granules (6:42), and 60% of the compound of formula (I)·nitenpyram water dispersible granules (10:50) showed good fast-acting properties, with control effects of 83.34% and 81.58%, respectively. %, 82.97%, 84.86%; 7 days after the application, the control efficacy of 64% formula (I) compound·thiamethoxam water dispersible granules (8:56), 56% formula (I) compound·acetamiprid water dispersible granules (7:49), 48% formula (I) compound·imidacloprid water dispersible granules (6:42), and 60% formula (I) compound·nitenpyram water dispersible granules (10:50) were improved, and the control efficacy was above 95%.

[0192] 1 day and 7 days after application, there was no significant difference in the control effect of 64% formula (I) compound·thiamethoxam water dispersible granules (8:56), 56% formula (I) compound·acetamiprid water dispersible granules (7:49), 48% formula (I) compound·imidacloprid water dispersible granules (6:42), and 60% formula (I) compound·nitenpyram water dispersible granules (10:50) on citrus aphids, but there was a significant difference between them and the other treatments, which was significantly higher than the control single agent and mixed preparation (20% imidacloprid·triazoctan wettable powder).

[0193] Table 11 The control effect of different pesticides on aphids

[0194]

[0195] Note: The above data are the average of 3 repetitions, with two decimal places, and the difference in efficacy is at the 0.05% level.

[0196] When using pesticides to control pests in the field, try to use them when the pests first occur, so that the control effect can be best.

[0197] Safety: It was observed that the test agent treatment did not cause any phytotoxicity or other adverse effects on citrus fruits compared with the blank control area.

[0198] The pesticide composition or formulation obtained by the present invention exhibits significant pest control efficacy, outperforming single-dose formulations in delaying the development of pesticide resistance and prolonging insecticide retention. Furthermore, no pesticide damage to crops was observed in the experiments, demonstrating that the pesticide composition or formulation, while enhancing insecticide synergy, can reduce production and use costs and is safe for crops.

[0199] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: It includes active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I) (I), the active ingredient B is thiamethoxam, and the mass ratio of the compound of formula (I) to thiamethoxam is 1:33~40:

1.

2. The pesticide composition according to claim 1, wherein: The mass ratio of the compound of formula (I) to thiamethoxam is 1:16-20:

1.

3. The pesticide composition according to claim 2, wherein: The mass ratio of the compound of formula (I) to thiamethoxam is 1:16, 1:12, 1:6, 2:1, 4:1, 10:1, 16:1, and 20:

1.

4. The pesticide composition according to claim 1, wherein: Based on the total mass of the pesticide composition as 100wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 0.5% to 90%.

5. The pesticide composition according to claim 4, characterized in that: Based on the total mass of the pesticide composition as 100wt%, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1% to 85%.

6. The pesticide composition according to claim 1, wherein: In addition to the active ingredients, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The pesticide composition according to claim 6, characterized in that: The pesticide composition is prepared into an agriculturally acceptable formulation, wherein the formulation is selected from solid formulations and / or liquid formulations; the solid formulation is selected from wettable powders and water-dispersible granules; and the liquid formulation is selected from emulsifiable concentrates, emulsions in water, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

8. Use of the pesticide composition according to any one of claims 1 to 7 in preventing and controlling agricultural, forestry and sanitary pests and mites.

9. The use according to claim 8, characterized in that: The agricultural, forestry and sanitary pests and mites are pests and mites on fruit trees, vegetables, ornamental plants, tea, cotton and cereal crops.

10. The use according to claim 9, characterized in that: The fruit trees are citrus and apple.

11. The pesticide composition according to claim 1, wherein: The pesticide composition is applied to the pests to be controlled or the medium where the pests grow at an effective dose.

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