Plant compound for inhibiting acne-related pathogenic bacteria and preparation method thereof

Abstract

The invention discloses a plant compound for inhibiting acne-related pathogenic bacteria and a preparation method thereof, and relates to the technical field of cosmetics. The plant compound is prepared from the following components in parts by weight: 20 to 30 parts of bilberry leaf extract, 25 to 30 parts of radix salviae miltiorrhizae extract, 12 to 20 parts of clove bud extract, 10 to 15 parts of radix scutellariae extract, 3 to 10 parts of cardiospermum halicacabum extract and 150 to 200 parts of water. According to the plant compound, the bilberry leaf extract, the salvia miltiorrhiza root extract, the clove bud extract, the scutellaria baicalensis root extract and the cardiospermum halicacabum extract are reasonably compounded, the five components are effectively utilized to synergistically inhibit acne-related pathogenic bacteria, the plant compound has a very good curative effect on acne removal, the preparation method of the plant compound is simple, the raw materials are easy to obtain, and the economic value is high.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a plant compound for inhibiting acne-related pathogens and a preparation method thereof. Background Art

[0002] Acne is a chronic disease of the pilosebaceous follicles and is one of the most common skin diseases in dermatology. Its clinical manifestations are characterized by polymorphic skin lesions such as acne, papules, pustules, and nodules on the face. The occurrence of acne is closely related to factors such as excessive sebum secretion, blockage of the pilosebaceous ducts, bacterial infection, and inflammatory response. When acne attacks, it will cause dilation of skin capillaries. After the acne subsides, the blood vessels cannot rebound quickly, forming erythema on the skin and even causing pigmentation, resulting in dull skin. Acne can cause skin bulges during the course of the disease, and after the disease subsides, it is often accompanied by dry and desquamated skin. Acne is common in adolescence and also occurs in people over 30 years old. Although acne is often accompanied by self-healing, it will cause psychological and physiological burdens to patients during the course of the disease, affecting the quality of life of patients.

[0003] Currently, acne treatment products on the market often use some chemically synthesized or even antibiotic ingredients to achieve the effect of removing acne. However, due to the chemical ingredients, long-term use of such acne treatment products often leads to bacterial resistance and other skin problems. Natural plant ingredients are known to be safe and widely available. Therefore, it is particularly important to develop a safe and efficient plant compound that inhibits acne-related pathogens. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention proposes a plant complex for inhibiting acne-related pathogens and a preparation method thereof to solve the problems raised by the above-mentioned background technology.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A plant complex for inhibiting acne-related pathogens. The plant complex comprises the following components by weight: 20 to 30 parts of blueberry leaf extract (leaves of Vaccinium vitis-idaea L., a plant of Ericaceae), 25 to 30 parts of salvia root extract (dried roots and rhizomes of Salvia miltiorrhiza Bunge., a plant of Lamiaceae), 12 to 20 parts of clove bud extract (Eugenia caryophyllus., a plant of Myrtaceae, Eugenia caryophyllata Thunb., a plant of Myrtaceae), 10 to 15 parts of scutellaria root extract (Scutellaria baicalensis., a perennial herb of the genus Scutellaria of the genus Scutellaria of the genus Lamiaceae), 3 to 10 parts of Cardiospermum halicacabum L., a plant of Sapindaceae, extract, and 150 to 200 parts of water.

[0007] Preferably, the plant complex comprises the following components by weight: 28 parts of bilberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0008] Preferably, the preparation method of the blueberry leaf extract comprises the following steps:

[0009] (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder;

[0010] (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g: (40-50) mL, placing under microwave conditions for microwave extraction for 50-90 min, and obtaining a microwave product;

[0011] (3) extracting the microwave product obtained in step (2) with ultrasound at a frequency of 60 to 70 kHz for 30 to 45 minutes, and filtering and separating after the ultrasonic extraction to obtain an extract;

[0012] (4) placing the extract obtained in step (3) in a rotary evaporator and performing reduced pressure distillation at 40-50° C. for 40 min to concentrate the extract, recovering ethanol to obtain an extract; and then freeze-drying the extract to obtain a blueberry leaf extract.

[0013] Preferably, the temperature of the microwave extraction in step (2) is 60° C. and the power of the microwave extraction is 500 W.

[0014] Preferably, the freeze-drying conditions of step (4) are to first place the product in a refrigerator at -30°C for 3 to 5 hours, and then immediately freeze-dry the product at -60°C for 1 to 3 hours.

[0015] Preferably, the preparation method of the Salvia miltiorrhiza root extract comprises the following steps: crushing the Salvia miltiorrhiza root, extracting it three times in water at 80-100°C, each time for 40-60 minutes, combining the three extracts, concentrating at 60-70°C to a relative density of 1.05-1.09, and spray drying to obtain the Salvia miltiorrhiza root extract; the mass of the water is 30-45 times the mass of the Salvia miltiorrhiza root, separating the Salvia miltiorrhiza root from the extract after each extraction, and recovering the Salvia miltiorrhiza root for the next extraction, and the volume ratio of the water for the three extractions is (7-10):(5-8):(4-7).

[0016] Preferably, the preparation method of the clove bud extract comprises the following steps: adding 70-80% ethanol by volume to the clove bud powder that has been dried and crushed to 200 meshes according to a material-liquid ratio of 1 g: (0.8-1.0) mL, extracting at room temperature for 10-20 min to obtain a clove bud liquid; then heating the clove bud liquid in a water bath at a vacuum degree of 0.1 Mpa-0.05 Mpa and a temperature of 45-55° C., and when bubbles appear and rise in the liquid, quickly adding 80-100 times the weight of the clove bud powder and 45-55° C. deionized water, and extracting for 30-50 min; after the extraction is completed, centrifuging at 10000-12000 r / min for 10 min, filtering through a 0.4 μm filter membrane to obtain a filtrate, and concentrating the filtrate to obtain a clove bud concentrate 3-5 times the weight of the clove bud powder, which is the clove bud extract.

[0017] Preferably, the method for preparing the scutellaria root extract comprises the following steps:

[0018] 1) Grinding: Take the root of Scutellaria baicalensis, grind it into coarse powder, and set aside;

[0019] 2) Extraction: Take the crude powder of step 1), add 75-80% ethanol in an amount of 10-15 times the total weight of the crude powder, heat and reflux at 70-80° C. for 1-3 times, each time for 45-90 minutes, filter after each extraction to obtain a filtrate, combine the obtained filtrates to obtain an extract; recover ethanol from the extract, place it at 70-80° C. and vacuum concentrate it to 1 / 5 of the original volume to obtain an extract;

[0020] 3) Column purification: Add the extract from step 2) to a polyamide column, and elute with 3 to 6 BV of ethanol solutions with volume fractions of 20%, 40%, 60%, and 80%, respectively, and collect the ethanol eluate with a volume fraction of 40%;

[0021] 4) Concentrating and drying: vacuum drying the 40% ethanol eluate in step 3) to obtain the Scutellaria baicalensis root extract.

[0022] Preferably, the preparation method of the extract of the Herba Lycopodii comprises the following steps: taking the whole herb of Herba Lycopodii, crushing it, adding 80% ethanol in an amount of 15 to 25 times the total weight of Herba Lycopodii, refluxing and extracting at 70 to 80°C for 1 to 3 times, each time for 30 to 45 minutes, filtering after each extraction to obtain a filtrate, combining the obtained filtrates, and vacuum drying to a moisture content of ≤6% to obtain the Herba Lycopodii extract.

[0023] Preferably, the method comprises the following steps: mixing components of blueberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract in parts by weight to obtain a mixed solution; performing reduced pressure distillation on the mixed solution at 60-75° C. to concentrate the mixed solution to a relative density of 1.0-1.35 g / ml to obtain the plant complex.

[0024] Preferably, the plant complex prepared by the present invention is used in the preparation of products for inhibiting acne.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] (1) The present invention rationally compounds blueberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract and cyperus rotundus extract, and effectively utilizes these five components to synergistically inhibit acne-related pathogens, thereby having a good therapeutic effect on eliminating acne. In addition, the preparation method of the plant complex is simple, the raw materials are easily available, and the economic value is high.

[0027] (2) The blueberry leaves are treated by a combination of microwaves and ultrasound to obtain blueberry leaf extract. The present invention first utilizes the electromagnetic field generated by microwaves to accelerate the diffusion rate of the molecules of the extracted components from the interior of the solid to the solid-liquid interface, rapidly increases the temperature of the sample and the solvent in a short period of time, accelerates the molecular movement, significantly shortens the extraction time, and accelerates the target components to enter the solvent. Since the temperature of the microwave extraction process does not exceed 60°C, and the ultrasonic extraction temperature has a smaller impact, the thermally stable components in the extract can be retained to the greatest extent, thereby increasing the content of the effective ingredients. Therefore, the present invention adopts the combined technology of microwave and ultrasound, integrating the advantages of the two extraction methods. The powerful combination of the two can reduce the extraction time, improve the extraction efficiency, and achieve the purpose of getting twice the result with half the effort.

[0028] (3) The preparation method of the extracts of each component of the present invention has the advantages of simple process, convenient operation, low cost, high extraction efficiency and high extraction rate. At the same time, the content of effective ingredients in the extracts of each component prepared thereby is very high and has high medicinal value. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0030] Example 1

[0031] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 20 parts of blueberry leaf extract, 25 parts of salvia miltiorrhiza root extract, 12 parts of clove bud extract, 10 parts of scutellaria baicalensis root extract, 3 parts of cyperus rotundus extract, and 150 parts of water.

[0032] The preparation method of the blueberry leaf extract comprises the following steps:

[0033] (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder;

[0034] (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g:40 mL, placing in a microwave condition at a temperature of 60° C. and a microwave extraction power of 500 W for 50 min to obtain a microwave product;

[0035] (3) extracting the microwave product obtained in step (2) with ultrasound at a frequency of 60 kHz for 30 minutes. After the ultrasonic extraction is completed, filtering and separating to obtain an extract;

[0036] (4) placing the extract obtained in step (3) in a rotary evaporator, performing reduced pressure distillation at 40° C. to concentrate the extract, recovering ethanol, and obtaining an extract; then freeze-drying the extract to obtain a blueberry leaf extract; the freeze-drying conditions are first refrigerating in a -30° C. refrigerator for 3 to 5 hours, and then immediately freeze-drying at -60° C. for 1 to 3 hours after taking out.

[0037] The preparation method of the salvia miltiorrhiza root extract comprises the following steps: crushing the salvia miltiorrhiza root, extracting it in 80°C water for 3 times, each time for 40 minutes, combining the 3 extracts, concentrating it at 60°C to a relative density of 1.05, and spray drying to obtain the salvia miltiorrhiza root extract; the mass of the water is 30 times the mass of the salvia miltiorrhiza root, separating the salvia miltiorrhiza root from the extract after each extraction, recovering the salvia miltiorrhiza root for the next extraction, and the volume ratio of the water for the 3 extractions is 7:5:4.

[0038] The preparation method of the clove bud extract comprises the following steps: adding 70% ethanol by volume to the clove bud powder that is dried and crushed to 200 meshes according to a material-liquid ratio of 1g:0.8mL, extracting at room temperature for 10min to obtain a clove bud material liquid; then heating the clove bud material liquid in a water bath at a vacuum degree of 0.1Mpa and a temperature of 45°C, and when bubbles appear and rise in the material liquid, quickly adding 45°C deionized water in an amount of 80 times the weight of the clove bud powder, and extracting for 30min; after the extraction is completed, centrifuging at 10000r / min for 10min, filtering through a 0.4μm filter membrane to obtain a filtrate, and concentrating the filtrate to obtain a clove bud concentrate in an amount of 3 times the weight of the clove bud powder, namely the clove bud extract.

[0039] The preparation method of the scutellaria root extract comprises the following steps:

[0040] 1) Grinding: Take the root of Scutellaria baicalensis, grind it into coarse powder, and set aside;

[0041] 2) Extraction: Take the crude powder of step 1), add 75% ethanol in an amount of 10 times the total weight of the crude powder, heat and reflux at 70°C for extraction once, each time for 45 minutes, filter after each extraction to obtain a filtrate, combine the obtained filtrates to obtain an extract; recover ethanol from the extract, place it at 70°C and vacuum concentrate it to 1 / 5 of the original volume to obtain an extract;

[0042] 3) Column purification: Add the extract from step 2) to a polyamide column, and elute with 3BV of ethanol solutions with a volume fraction of 20%, 40%, 60%, and 80%, and collect the ethanol eluate with a volume fraction of 40%;

[0043] 4) Concentrating and drying: vacuum drying the 40% ethanol eluate in step 3) to obtain the Scutellaria baicalensis root extract.

[0044] The preparation method of the extract of the Herba Lycopodii comprises the following steps: taking the whole herb of the Herba Lycopodii, crushing it, adding 80% ethanol with a volume fraction of 15 times the total weight of the Herba Lycopodii, refluxing and extracting it once at 70° C. for 30 minutes each time, filtering to obtain a filtrate after each extraction, combining the obtained filtrates, and vacuum drying to a moisture content of ≤6% to obtain the Herba Lycopodii extract.

[0045] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 60°C to concentrate the mixed solution to a relative density of 1.0 g / ml to obtain the plant composite.

[0046] Example 2

[0047] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 30 parts of blueberry leaf extract, 30 parts of salvia miltiorrhiza root extract, 20 parts of clove bud extract, 15 parts of scutellaria baicalensis root extract, 10 parts of cyperus rotundus extract, and 200 parts of water.

[0048] The preparation method of the bilberry leaf extract comprises the following steps:

[0049] (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder;

[0050] (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g:50 mL, placing the mixture under microwave conditions of 60° C. and 500 W microwave extraction power for 90 min to obtain a microwave product;

[0051] (3) extracting the microwave product obtained in step (2) with ultrasound at a frequency of 70 kHz for 45 minutes. After the ultrasonic extraction is completed, filtering and separating to obtain an extract;

[0052] (4) placing the extract obtained in step (3) in a rotary evaporator, performing reduced pressure distillation at 50° C. to concentrate the extract, recovering ethanol, and obtaining an extract; then freeze-drying the extract to obtain a blueberry leaf extract; the freeze-drying conditions are first refrigerating in a -30° C. refrigerator for 3 to 5 hours, and then immediately freeze-drying at -60° C. for 1 to 3 hours after taking out.

[0053] The preparation method of the salvia miltiorrhiza root extract comprises the following steps: crushing the salvia miltiorrhiza root, extracting it in 100°C water for 3 times, each time for 60 minutes, combining the 3 extracts, concentrating it at 70°C to a relative density of 1.09, and spray drying to obtain the salvia miltiorrhiza root extract; the mass of the water is 45 times the mass of the salvia miltiorrhiza root, separating the salvia miltiorrhiza root from the extract after each extraction, recovering the salvia miltiorrhiza root for the next extraction, and the volume ratio of the water in the 3 extractions is 10:8:7.

[0054] The preparation method of the clove bud extract comprises the following steps: adding ethanol with a volume concentration of 80% to the clove bud powder that is dried and crushed to 200 meshes according to a material-liquid ratio of 1g:1.0mL, extracting at room temperature for 20 minutes to obtain a clove bud material liquid; then heating the clove bud material liquid in a water bath at a vacuum degree of 0.05Mpa and a temperature of 55°C, and when bubbles appear and rise in the material liquid, quickly adding 55°C deionized water with a weight of 100 times that of the clove bud powder, and extracting for 50 minutes; after the extraction is completed, centrifuging at 12000r / min for 10 minutes, filtering through a 0.4μm filter membrane to obtain a filtrate, and concentrating the filtrate to obtain a clove bud concentrated solution with a weight of 5 times that of the clove bud powder, namely the clove bud extract.

[0055] The preparation method of the scutellaria root extract comprises the following steps:

[0056] 1) Grinding: Take the root of Scutellaria baicalensis, grind it into coarse powder, and set aside;

[0057] 2) Extraction: Take the crude powder of step 1), add ethanol with a volume fraction of 80% in an amount 15 times the total weight of the crude powder, heat and reflux for extraction at 80°C for 3 times, each time for 90 minutes, filter after each extraction to obtain a filtrate, combine the obtained filtrates to obtain an extract; recover ethanol from the extract, place it at 80°C and vacuum concentrate it to 1 / 5 of the original volume to obtain an extract;

[0058] 3) Column purification: Add the extract from step 2) to a polyamide column, and elute with 6BV ethanol solutions with a volume fraction of 20%, 40%, 60%, and 80%, and collect the ethanol eluate with a volume fraction of 40%;

[0059] 4) Concentrating and drying: vacuum drying the 40% ethanol eluate in step 3) to obtain the Scutellaria baicalensis root extract.

[0060] The preparation method of the extract of the Herba Lycopodii comprises the following steps: taking the whole herb of the Herba Lycopodii, crushing it, adding 80% ethanol with a volume fraction of 25 times the total weight of the Herba Lycopodii, refluxing and extracting at 80° C. for 3 times, each time for 45 minutes, filtering after each extraction to obtain a filtrate, combining the obtained filtrates, and vacuum drying until the moisture content is ≤6%, thereby obtaining the Herba Lycopodii extract.

[0061] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 75°C to concentrate the mixed solution to a relative density of 1.35 g / ml to obtain the plant composite.

[0062] Example 3

[0063] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of blueberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0064] The preparation method of the bilberry leaf extract comprises the following steps:

[0065] (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder;

[0066] (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g:45 mL, placing the mixture under microwave conditions of 60° C. and 500 W microwave extraction power for 70 min to obtain a microwave product;

[0067] (3) extracting the microwave product obtained in step (2) with ultrasound at a frequency of 65 kHz for 40 minutes. After the ultrasonic extraction is completed, filtering and separating to obtain an extract;

[0068] (4) placing the extract obtained in step (3) in a rotary evaporator, performing reduced pressure distillation at 45° C. to concentrate the extract, recovering ethanol, and obtaining an extract; then freeze-drying the extract to obtain a blueberry leaf extract; the freeze-drying conditions are first refrigerating in a -30° C. refrigerator for 3 to 5 hours, and then immediately freeze-drying at -60° C. for 1 to 3 hours after taking out.

[0069] The preparation method of the salvia miltiorrhiza root extract comprises the following steps: crushing the salvia miltiorrhiza root, extracting it in 90°C water for 3 times, each time for 50 minutes, combining the 3 extracts, concentrating it at 65°C to a relative density of 1.08, and spray drying to obtain the salvia miltiorrhiza root extract; the mass of the water is 40 times the mass of the salvia miltiorrhiza root, separating the salvia miltiorrhiza root from the extract after each extraction, recovering the salvia miltiorrhiza root for the next extraction, and the volume ratio of the water for the 3 extractions is 8:7:5.

[0070] The preparation method of the clove bud extract comprises the following steps: adding 75% ethanol by volume to the clove bud powder that is dried and crushed to 200 meshes according to a material-liquid ratio of 1g:0.9mL, extracting at room temperature for 15min to obtain a clove bud material liquid; then heating the clove bud material liquid in a water bath at a vacuum degree of 0.3Mpa and a temperature of 50°C, and when bubbles appear and rise in the material liquid, quickly adding 50°C deionized water in an amount of 90 times the weight of the clove bud powder, and extracting for 40min; after the extraction is completed, centrifuging at 11000r / min for 10min, filtering through a 0.4μm filter membrane to obtain a filtrate, and concentrating the filtrate to obtain a clove bud concentrate in an amount of 4 times the weight of the clove bud powder, namely the clove bud extract.

[0071] The preparation method of the scutellaria root extract comprises the following steps:

[0072] 1) Grinding: Take the root of Scutellaria baicalensis, grind it into coarse powder, and set aside;

[0073] 2) Extraction: Take the crude powder of step 1), add 78% ethanol in an amount of 12 times the total weight of the crude powder, heat and reflux for extraction twice at 75°C, each time for 75 minutes, filter after each extraction to obtain a filtrate, combine the obtained filtrates to obtain an extract; recover ethanol from the extract, place it at 75°C and vacuum concentrate it to 1 / 5 of the original volume to obtain an extract;

[0074] 3) Column purification: Add the extract from step 2) to a polyamide column, and elute with 5BV of ethanol solutions with a volume fraction of 20%, 40%, 60%, and 80%, respectively, and collect the ethanol eluate with a volume fraction of 40%;

[0075] 4) Concentrating and drying: vacuum drying the 40% ethanol eluate in step 3) to obtain the Scutellaria baicalensis root extract.

[0076] The preparation method of the extract of the Herba Lycopodii comprises the following steps: taking the whole herb of the Herba Lycopodii, crushing it, adding 80% ethanol with a volume fraction of 20 times the total weight of the Herba Lycopodii, refluxing and extracting it twice at 75° C., each time for 40 minutes, filtering after each extraction to obtain a filtrate, combining the obtained filtrates, and vacuum drying until the moisture content is ≤6%, thereby obtaining the Herba Lycopodii extract.

[0077] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant composite.

[0078] Comparative Example 1

[0079] The difference between this comparative example and Example 3 is that the blueberry leaf extract is not extracted by microwave + ultrasound, and the other components and steps remain unchanged.

[0080] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of blueberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0081] The preparation method of the bilberry leaf extract comprises the following steps:

[0082] (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder;

[0083] (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g:45 mL, extracting for 110 min, and filtering to obtain an extract;

[0084] (3) placing the extract obtained in step (2) in a rotary evaporator, performing reduced pressure distillation at 45° C. to concentrate the extract, recovering ethanol to obtain an extract; then freeze-drying the extract to obtain a blueberry leaf extract; the freeze-drying conditions are first refrigerating in a -30° C. refrigerator for 3 to 5 hours, and then immediately freeze-drying at -60° C. for 1 to 3 hours after taking out.

[0085] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant composite.

[0086] Comparative Example 2

[0087] This comparative example is different from Example 3 in that the root of Salvia miltiorrhiza is replaced with Sophora flavescens, and the other ingredients and steps remain unchanged.

[0088] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of bilberry leaf extract, 27 parts of sophora flavescens extract, 18 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0089] The preparation method of the Sophora flavescens extract comprises the following steps: crushing Sophora flavescens, extracting it in water at 90° C. for 3 times, each time for 50 minutes, combining the 3 extracts, concentrating it at 65° C. to a relative density of 1.08, and spray drying to obtain the Sophora flavescens extract; the mass of the water is 40 times the mass of the root of Salvia miltiorrhiza, separating the Sophora flavescens from the extract after each extraction, recovering the Sophora flavescens for the next extraction, and the volume ratio of the water for the 3 extractions is 8:7:5.

[0090] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, sophora flavescens extract, clove bud extract, scutellaria baicalensis root extract, and cyperus rotundus extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant composite.

[0091] Comparative Example 3

[0092] This comparative example differs from Example 3 in that: the weight portion of the extracted clove buds is increased, and the other ingredients and steps remain unchanged.

[0093] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of bilberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 36 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0094] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cyperus rotundus extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant composite.

[0095] Comparative Example 4

[0096] The difference between this comparative example and Example 3 is that the scutellaria root extract component is not added to the plant complex, and the other components and steps remain unchanged.

[0097] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of bilberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

[0098] A method for preparing a plant composite for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, and cycas revoluta extract with water in parts by weight to obtain a mixed solution; and performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant composite.

[0099] Comparative Example 5

[0100] The difference between this comparative example and Example 3 is that: the difference between this comparative example and Example 3 is that the component of the extract of Cyperus rotundus is not added to the plant complex, and the other ingredients and steps remain unchanged.

[0101] A plant complex for inhibiting acne-related pathogens, comprising the following components by weight: 28 parts of bilberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 12 parts of scutellaria root extract and 180 parts of water.

[0102] A method for preparing a plant complex for inhibiting acne-related pathogens comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract and water in parts by weight to obtain a mixed solution; performing reduced pressure distillation on the mixed solution at 70°C to concentrate the mixed solution to a relative density of 1.25 g / ml to obtain the plant complex.

[0103] Experimental Example 1 Antibacterial Test

[0104] Staphylococcus aureus ATCC6538 was inoculated into TSB medium, and the bacterial solution was aerobically cultured at 37°C for 24 h. 7 cfu / ml. 4.5 ml of each diluted bacterial solution, 9 portions each, were added with 0.5 ml of the plant complex of Examples 1 to 3, the plant complex of Comparative Examples 1 to 5, deionized water (blank control) and erythromycin ointment (the main ingredient is erythromycin, positive control), three parallel groups, and then the bacterial solution was placed at 37°C for aerobic culture for 24 hours.

[0105] Propionibacterium acnes ATCC6919 and Propionibacterium acnes ATCC11827 were inoculated into BHI culture medium, and the culture medium was placed in anaerobically cultured at 37°C for 48 hours. 7Each diluted bacterial solution was 4.5 ml, and 9 portions were added with 0.5 ml of the plant complex of Examples 1 to 3, the plant complex of Comparative Examples 1 to 5, and deionized water (deionized water was used as a blank control), respectively, in 3 parallel groups, and then the bacterial solution was placed at 37°C for anaerobic culture for 48 hours.

[0106] The number of various bacteria in the culture solution before and after cultivation was determined by the dilution plate method, and the average value was taken, as shown in Table 1:

[0107] Table 1 Changes in the number of Staphylococcus aureus and Propionibacterium acnes before and after culture (cfu / ml)

[0108]

[0109]

[0110] As shown in Table 1, the plant complex prepared by the present invention can inhibit the growth of Staphylococcus aureus and Propionibacterium acnes, especially the best inhibitory effect in Example 3. Comparison between Example 3 and Comparative Example 1 shows that the blueberry leaf extract obtained by microwave and ultrasonic treatment has high antibacterial active ingredients such as arbutin, pyrin, salvinia glycosides and quercetin 3-D-glucose-L-rhamnoside, which can have a good inhibitory effect on acne-related pathogens; Comparison between Example 3 and Comparative Example 2 shows that the present invention has strict requirements on the types of components. Even though the roots of Salvia miltiorrhiza and Sophora flavescens are both dried roots, Sophora flavescens has the effects of clearing away heat and detoxifying, anti-inflammatory and antibacterial, but the inhibitory effect of Salvia miltiorrhiza root on acne is more obvious than that of Sophora flavescens; Comparison between Example 3 and Comparative Example 2 shows that the present invention has strict requirements on the types of components. Even though the roots of Salvia miltiorrhiza and Sophora flavescens are both dried roots, Sophora flavescens has the effects of clearing heat and detoxifying, anti-inflammatory and antibacterial, but the root of Salvia miltiorrhiza is selected as the component, which has a more obvious inhibitory effect on acne than Sophora flavescens; Comparison with Comparative Example 3 shows that the present invention has strict requirements on the number of components. Even if the number of clove bud extract is increased, not only the antibacterial effect is not improved, but Staphylococcus aureus and Propionibacterium acnes grow slowly. Comparison of Example 3 with Comparative Example 4 and Comparative Example 5 shows that the present invention has certain requirements on the integrity of the components. There is a synergistic effect between the blueberry leaf extract, the salvia miltiorrhiza root extract, the clove bud extract, the scutellaria root extract and the cyperus rotundus extract, which jointly fight against pathogens. When a certain component is missing, the synergistic effect is broken, resulting in a significant reduction in the antibacterial effect.

[0111] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant compound for inhibiting acne-related pathogens, characterized in that: The plant complex comprises the following components by weight: 20-30 parts of blueberry leaf extract, 25-30 parts of salvia miltiorrhiza root extract, 12-20 parts of clove bud extract, 10-15 parts of scutellaria root extract, 3-10 parts of cyperus rotundus extract and 150-200 parts of water.

2. The plant complex according to claim 1, characterized in that The plant complex comprises the following components by weight: 28 parts of blueberry leaf extract, 27 parts of salvia miltiorrhiza root extract, 18 parts of clove bud extract, 12 parts of scutellaria baicalensis root extract, 7 parts of cyperus rotundus extract, and 180 parts of water.

3. The plant complex according to claim 1, characterized in that The preparation method of the blueberry leaf extract comprises the following steps: (1) Select fresh blueberry leaves, wash and crush them to obtain blueberry leaf powder; (2) mixing blueberry leaf powder with anhydrous ethanol solution at a mass volume ratio of 1 g: (40-50) mL, placing under microwave conditions for microwave extraction for 50-90 min, and obtaining a microwave product; (3) extracting the microwave product obtained in step (2) with ultrasound at a frequency of 60 to 70 kHz for 30 to 45 minutes, and filtering and separating after the ultrasonic extraction to obtain an extract; (4) placing the extract obtained in step (3) in a rotary evaporator and performing reduced pressure distillation at 40-50° C. to concentrate the extract, recover ethanol, and obtain an extract; then freeze-drying the extract to obtain a blueberry leaf extract.

4. The plant complex according to claim 3, characterized in that The temperature of the microwave extraction in step (2) is 60° C. and the power of the microwave extraction is 500 W.

5. The plant complex according to claim 1, characterized in that The preparation method of the salvia miltiorrhiza root extract comprises the following steps: crushing the salvia miltiorrhiza root, extracting it three times in water at 80-100° C., each time for 40-60 minutes, combining the three extracts, concentrating at 60-70° C. to a relative density of 1.05-1.09, and spray drying to obtain the salvia miltiorrhiza root extract; the mass of the water is 30-45 times the mass of the salvia miltiorrhiza root, separating the salvia miltiorrhiza root from the extract after each extraction, recovering the salvia miltiorrhiza root for the next extraction, and the volume ratio of the water in the three extractions is (7-10):(5-8):(4-7).

6. The plant complex according to claim 1, characterized in that The preparation method of the clove bud extract comprises the following steps: adding ethanol with a volume concentration of 70-80% to the clove bud powder that is dried and crushed to 200 meshes according to a material-liquid ratio of 1g:(0.8-1.0)mL, extracting at room temperature for 10-20min to obtain a clove bud material liquid; then heating the clove bud material liquid in a water bath at a vacuum degree of 0.1Mpa-0.05Mpa and a temperature of 45-55°C, and when bubbles appear and rise in the material liquid, quickly adding 45-55°C deionized water with a weight of 80-100 times that of the clove bud powder, and extracting for 30-50min; after the extraction is completed, centrifuging at 10000-12000r / min for 10min, filtering through a 0.4μm filter membrane to obtain a filtrate, and concentrating the filtrate to obtain a clove bud concentrated solution with a weight of 3-5 times that of the clove bud powder, namely the clove bud extract.

7. The plant complex according to claim 1, characterized in that The preparation method of the scutellaria root extract comprises the following steps: 1) Grinding: Take the root of Scutellaria baicalensis, grind it into coarse powder, and set aside; 2) Extraction: Take the crude powder of step 1), add 75-80% ethanol in an amount of 10-15 times the total weight of the crude powder, heat and reflux at 70-80° C. for 1-3 times, each time for 45-90 minutes, filter after each extraction to obtain a filtrate, combine the obtained filtrates to obtain an extract; recover ethanol from the extract, place it at 70-80° C. and vacuum concentrate it to 1 / 5 of the original volume to obtain an extract; 3) Column purification: Add the extract from step 2) to the polyamide column and use 3-6 BV of volume fractions of 20%, 40%, 60% and 80% ethanol solutions were used for elution, and the ethanol eluate with a volume fraction of 40% was collected; 4) Concentrating and drying: vacuum drying the 40% ethanol eluate in step 3) to obtain the scutellaria root extract.

8. The plant complex according to claim 1, characterized in that The preparation method of the extract of the Herba Lycopodii comprises the following steps: taking the whole herb of the Herba Lycopodii, crushing it, adding 80% ethanol in an amount of 15 to 25 times the total weight of the Herba Lycopodii, refluxing and extracting at 70 to 80° C. for 1 to 3 times, each time for 30 to 45 minutes, filtering after each extraction to obtain a filtrate, combining the obtained filtrates, and vacuum drying until the moisture content is ≤6%, thereby obtaining the Herba Lycopodii extract.

9. The method for preparing the plant complex according to claim 1, characterized in that: The method comprises the following steps: mixing components of bilberry leaf extract, salvia miltiorrhiza root extract, clove bud extract, scutellaria root extract, and cycas revoluta extract with water in parts by weight to obtain a mixed solution; performing reduced pressure distillation on the mixed solution at 60-75°C to concentrate the mixed solution to a relative density of 1.0-1.35 g / ml to obtain a plant complex.

10. Use of the plant complex according to any one of claims 1 to 8 or the plant complex prepared by the preparation method according to claim 9 in preparing an acne-inhibiting product.

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