Apocynum venetum extract composition, preparation method and application of apocynum venetum extract composition in nerve calming and sleep aiding products

Abstract

The invention relates to the technical field of plant extracts, in particular to an apocynum venetum extract composition, a preparation method and application of the apocynum venetum extract composition in nerve soothing and sleep aiding products. The composition is prepared from the following raw materials in parts by weight: 30 to 50 parts of semen ziziphi spinosae, 20 to 30 parts of bulbus lilii, 5 to 10 parts of modified zein nanoparticles loaded with apocynum venetum extract, 4 to 6 parts of theanine and 1 to 3 parts of vitamin B6. The invention also provides a preparation method of the composition. Compared with the prior art, the composition prepared by the invention can effectively shorten the sleep latency and prolong the sleep time, and has good effects of soothing the nerves and helping sleep.

Description

Technical Field

[0001] The present invention relates to the technical field of plant extracts, and in particular to a dogbane extract composition, a preparation method and application thereof in a product for calming nerves and promoting sleep. Background Art

[0002] Insomnia, also known as sleep disorder, is a disease characterized by frequent difficulty falling asleep and / or sleep interruptions, which causes individuals to feel insufficient sleep satisfaction. It has become a public health issue of widespread concern in modern society. Clinically, insomnia is mainly manifested as difficulty falling asleep, sleep interruptions, waking up too early, decreased sleep quality, and reduced total sleep time, accompanied by impaired daytime function. As a chronic disease that is difficult to cure completely, insomnia not only has a long course of illness, but in severe cases may induce or aggravate cardiovascular and cerebrovascular diseases, and is often closely related to mental health problems. With the accelerated pace of modern life and the increase of various social pressures, the incidence of insomnia has shown an upward trend in recent years.

[0003] Benzodiazepines BZDs include diazepam (Valium), chlordiazepoxide (Chlordiazepoxide), nitrazepam (nitrazepam), estazolam (sulosazone), etc. can exert their effects by non-selectively stimulating different α subunits on the γ-aminobutyric acid receptor A (GABAA), and have pharmacological effects such as hypnosis, anxiolysis, antispasmodic and muscle relaxation. Non-BZDs include zolpidem and eszopiclone, which have similar hypnotic effects to BZDs, but due to their short half-life, the residual effect the next day is minimized, and they generally do not cause daytime sleepiness, so the risk of drug dependence is lower than that of traditional BZDs. Melatonin receptor agonists such as ramelton and agomelatine, melatonin can regulate circadian rhythms and sleep through specific receptors; agomelatine is both a melatonin receptor agonist and an antagonist of 5-hydroxytryptamine 2c (5-HT2c) receptors, so it has both antidepressant and sleep-improving effects. Including methadone, amitriptyline, etc., in addition to their analgesic or antidepressant effects, they also have sedative and hypnotic effects, and are suitable for the treatment of insomnia accompanied by pain symptoms or depressive symptoms. It should be noted that these drugs have certain side effects and potential addiction, so the risks are relatively high.

[0004] Therefore, taking natural ingredients instead of drugs will have a safer effect of calming the nerves and helping sleep. In recent years, extracts of Chinese date seeds, lavender, etc. have been frequently added to various health products for calming the nerves. Natural ingredients will not cause the body to become dependent on them. In addition to calming the nerves and helping sleep, many natural ingredients also have other health benefits, such as anti-depression, enhancing immunity, etc. For people who are unwilling or unable to take drugs, natural ingredients provide another way to improve sleep.

[0005] CN102389141A provides a soothing and sleep-inducing beverage and a preparation method thereof, which is composed of the following substances in proportion by mass: 80-200 parts of a jujube seed extract containing 1-2.5% of jujube seed total saponins; 10-50 parts of a lavender extract with a lavandin content of 6-15%; 1-3 parts each of vitamin B1, B2, and B6, and 10-30 parts of vitamin C; 1000-3000 parts of nano soybean meal with a particle size distribution in the range of 70-800nm; and 8-10 million parts of drinking water. Dissolve the jujube seed extract in drinking water to make liquid A; dissolve the lavender extract in drinking water to make liquid B; take vitamin B1, B2, and B6 and dissolve them in drinking water at 80°C to make liquid C; take vitamin C and dissolve it in drinking water to make liquid D; dissolve the nano soybean meal in drinking water, add the above liquids A, B, C, and D in turn, mix and stir for 0.5-12 hours, heat to 80°C and keep warm for 1-6 minutes to sterilize, add drinking water, mix evenly, sterilize, and aseptically fill. The invention uses nano soybean meal to absorb active ingredients, is not easily affected by external conditions such as light and temperature, has greatly improved stability, and saves energy.

[0006] CN116712486A discloses a Xanthoceras sorbifolia herbal composition for calming nerves and promoting sleep in the field of traditional Chinese medicine technology and a preparation method thereof, comprising the following components in parts by weight: 30-50 parts of Xanthoceras sorbifolia shell extract microcapsules, 10-30 parts of Ziziphus jujuba seeds, 5-15 parts of Poria cocos, 10-30 parts of Salvia miltiorrhiza, 6-10 parts of oysters, and 5-10 parts of dragon bones; the Xanthoceras sorbifolia shell extract microcapsules are prepared by subjecting Xanthoceras sorbifolia shells to microwave-assisted enzymatic hydrolysis and purification; in order to solve the problem of poor sleep quality for some people after entering a plateau environment, the invention proposes a method of purifying the Xanthoceras sorbifolia shell extract and preparing microcapsules, thereby achieving the effectiveness and stability of the extract, improving the body's hypoxia tolerance after use, and thus achieving the technical effect of calming nerves and promoting sleep; and at the same time, a plurality of traditional Chinese medicines are mixed with the microcapsules to prepare the composition, thereby further enhancing the technical effect of calming nerves and promoting sleep.

[0007] Despite the aforementioned advantages of natural ingredients, the active ingredients in extracts are not very stable and may therefore easily decompose during storage, resulting in reduced efficacy. Summary of the invention

[0008] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a composition of apocynum venetum extract, a preparation method and application thereof in a product for calming the nerves and promoting sleep.

[0009] Hyperoside and isoquercetin in Apocynum venetum extract have the effect of improving sleep. At the same time, Apocynum venetum extract combined with GABA can also help relieve stress. As the main active ingredients in Apocynum venetum leaves, hyperoside and isoquercetin have good pharmacological activity. Apocynum venetum extract can promote the biosynthesis of 5-hydroxytryptamine, thereby helping to improve sleep. 5-hydroxytryptamine is an important neurotransmitter that is closely related to mood and sleep regulation. Hyperoside and isoquercetin may exert their sleep-inducing effect by affecting the level of 5-hydroxytryptamine. GABA (γ-aminobutyric acid) is a major inhibitory neurotransmitter in the central nervous system. It reduces the excitability of neurons by binding to GABA receptors, thereby producing sedative and anti-anxiety effects. After hyperoside and isoquercetin in Apocynum venetum extract combine with GABA, they may enhance the effect of GABA and help relieve stress. This combination may help relieve stress and anxiety by enhancing the inhibitory effect of GABA and reducing the excitability of the brain.

[0010] However, hyperoside and isoquercetin will degrade to varying degrees under high temperature, high humidity or light conditions, and isoquercetin is almost insoluble in water. Therefore, the present invention provides a kind of apocynum venetum extract nanoparticles, using modified zein as a carrier, because the isoelectric point of zein is 6.2-6.8, the prepared nanoparticles have poor colloidal stability in aqueous medium, and the single prepared zein drug-loaded particles have the disadvantages of low encapsulation rate, poor water solubility, poor stability and poor redispersibility after freeze-drying, so the present invention uses amino acids to modify it, so that the conformation of zein changes, thereby causing protein subunit decomposition, improving the solubility of zein, and zein itself has unique self-assembly characteristics, so it can be used as an encapsulation delivery carrier, which helps to encapsulate hydrophilic, hydrophobic and amphiphilic bioactive substances. The grafted zein has better solubility, so the encapsulation rate and dispersibility are improved, and the protection of the apocynum venetum extract and the role of the effective ingredients are also stronger.

[0011] By encapsulating the Apocynum venetum extract in modified zein, it can easily bind to the γ-aminobutyric acid grafted on the surface of zein when released. After hyperoside and isoquercetin bind to GABA, they may enhance the effect of GABA, thereby achieving a more effective tranquilizing and sleep-inducing effect.

[0012] To achieve the above object, the present invention provides a composition of apocynum venetum extract, comprising the following raw materials in parts by weight: 30-50 parts of spiny jujube seeds, 20-30 parts of lilies, 5-10 parts of modified zein nanoparticles loaded with apocynum venetum extract, 4-6 parts of theanine and 1-3 parts of vitamin B6.

[0013] The method for preparing the modified zein nanoparticles loaded with apocynum venetum extract comprises the following steps:

[0014] S1, dissolving zein in an ethanol aqueous solution, adding amino acids, and then adding 4-dimethylaminopyridine and triethylamine, dialyzing and freeze-drying after the reaction to obtain modified zein;

[0015] S2. The modified zein and the Apocynum venetum extract are subjected to an anti-solvent precipitation method to obtain modified zein nanoparticles loaded with the Apocynum venetum extract.

[0016] Furthermore, the method for preparing the modified zein nanoparticles loaded with apocynum venetum extract comprises the following steps:

[0017] S1, dissolving zein in a 70-80 wt% ethanol aqueous solution, adding amino acids, and then adding 4-dimethylaminopyridine and triethylamine, stirring at 20-30° C. for 20-30 h, dialyzing for 2-3 days, and freeze-drying to obtain modified zein;

[0018] S2. Dissolving the modified zein and the apocynum venetum extract in a 70-80 wt% ethanol aqueous solution to obtain an organic phase, adding the organic phase to 9-10 times the volume of water under stirring, standing after stirring, and freeze-drying to obtain modified zein nanoparticles loaded with the apocynum venetum extract.

[0019] Preferably, the mass ratio of the zein to the ethanol aqueous solution is 1:50-100.

[0020] Preferably, the mass ratio of zein to amino acid, 4-dimethylaminopyridine and triethylamine is 1:0.1-1:0.01-0.2:0.1-0.5.

[0021] Preferably, the molecular weight of the dialyzed molecules is <5000Da.

[0022] Preferably, the amino acid is one of lysine, γ-aminobutyric acid and leucine.

[0023] Preferably, the mass ratio of the modified zein to the Apocynum venetum extract and the ethanol aqueous solution is 20-30:1:100-500.

[0024] The invention also provides a method for preparing the apocynum venetum extract composition.

[0025] A method for preparing a composition of apocynum venetum extract comprises the following steps:

[0026] The spiny jujube seeds and lily are crushed and mixed with modified zein nanoparticles loaded with apocynum venetum extract, theanine and vitamin B6 until evenly mixed to obtain the product.

[0027] The present invention also provides an application of the Apocynum venetum extract composition in a product for calming nerves and promoting sleep, such as a probiotic powder or a drink.

[0028] Beneficial effects of the present invention:

[0029] The Chinese date seed contains various pharmacological ingredients such as alkaloids, saponins and flavonoids, and has sedative, hypnotic, analgesic, anticonvulsant and other effects. Lily has the effect of prolonging sleep time and calming the nerves. Hyperoside and isoquercitrin in the apocynum extract have the effect of improving sleep. At the same time, modified zein nanoparticles as a carrier can improve the bioavailability and stability of the apocynum extract, making it more effective. Theanine can help enter a deeper sleep, thereby better repairing physical fatigue. Vitamin B6 participates in the synthesis of various neurotransmitters, including 5-hydroxytryptamine (serotonin) and melatonin, and has a positive effect on sleep. It helps to regulate the nervous system, reduce anxiety and improve sleep quality. These components work together in the apocynum extract composition, which helps to calm the nerves and help sleep, and improve sleep quality. DETAILED DESCRIPTION

[0030] A composition of apocynum venetum extract comprises the following raw materials: jujube seeds, lily, modified zein nanoparticles loaded with apocynum venetum extract, theanine and vitamin B6. The jujube seeds contain various pharmacological components such as alkaloids, saponins and flavonoids, and have sedative, hypnotic, analgesic, anticonvulsant and other effects. Spinosin and jujube seed saponin A are the main components of the sedative and hypnotic activity of the jujube seeds. The lily has the effects of prolonging sleep time and calming the nerves. Hyperoside and isoquercitrin in the apocynum venetum extract have the effect of improving sleep. At the same time, the modified zein nanoparticles as a carrier can improve the bioavailability and stability of the apocynum venetum extract, making it more effective. Theanine is a unique amino acid present in tea leaves, accounting for 50%-60% of the total amino acids in tea leaves. Theanine can help enter a deeper sleep, thereby better repairing physical fatigue. Vitamin B6 participates in the synthesis of various neurotransmitters, including 5-hydroxytryptamine (serotonin) and melatonin, and has a positive effect on sleep. It helps regulate the nervous system, reduce anxiety and improve sleep quality. These components work together in the composition of the dogbun extract to help calm the nerves and improve sleep quality.

[0031] Apocynum venetum extract, specification: 10:1, 100 mesh, Xi'an Aosai Biotechnology Co., Ltd.

[0032] Example 1

[0033] A method for preparing a composition of apocynum venetum extract comprises the following steps, measured in parts by weight:

[0034] 40 parts of sour jujube seeds and 25 parts of lily are crushed into 100 meshes, and then mixed with 7 parts of modified zein nanoparticles loaded with apocynum venetum extract, 5 parts of theanine and 2 parts of vitamin B6, and mixed evenly to obtain a mixture;

[0035] The method for preparing modified zein nanoparticles loaded with apocynum venetum extract comprises the following steps, measured in parts by weight:

[0036] S1, dissolving 5 parts of zein in 400 parts of 80wt% ethanol aqueous solution, adding 1 part of lysine, and then adding 0.6 parts of 4-dimethylaminopyridine and 1 part of triethylamine, stirring at 25°C for 24 hours, dialyzing (Mw < 5000Da) for 2 days, and freeze-drying to obtain modified zein;

[0037] S2. Dissolve 25 parts of modified zein and 1 part of Apocynum venetum extract in 500 parts of 80wt% ethanol aqueous solution to obtain an organic phase, add the organic phase into 10 times the volume of water under stirring, let stand after stirring, and freeze-dry to obtain modified zein nanoparticles loaded with Apocynum venetum extract.

[0038] Example 2

[0039] The method is substantially the same as Example 1, except that 1 part of lysine is replaced by 0.9 part of leucine.

[0040] Example 3

[0041] The method is substantially the same as Example 1, except that lysine is replaced by 0.7 parts of γ-aminobutyric acid.

[0042] Comparative Example 1

[0043] A method for preparing a composition of apocynum venetum extract comprises the following steps, measured in parts by weight:

[0044] 40 parts of sour jujube seeds and 25 parts of lily are crushed into 100 meshes, and then mixed with 7 parts of zein nanoparticles loaded with apocynum venetum extract, 5 parts of theanine and 2 parts of vitamin B6, and mixed evenly to obtain a mixture;

[0045] The method for preparing zein nanoparticles loaded with apocynum venetum extract comprises the following steps, measured in parts by weight:

[0046] 25 parts of zein and 1 part of Apocynum venetum extract were dissolved in 500 parts of 80wt% ethanol aqueous solution to obtain an organic phase, and the organic phase was added to 10 times the volume of water under stirring, and then allowed to stand after stirring, and freeze-dried to obtain zein nanoparticles loaded with Apocynum venetum extract.

[0047] Comparative Example 2

[0048] A method for preparing a composition of apocynum venetum extract comprises the following steps, measured in parts by weight:

[0049] 40 parts of sour jujube seeds and 25 parts of lilies are crushed into 100 meshes, and then mixed with 7 parts of apocynum venetum extract, 5 parts of theanine and 2 parts of vitamin B6, and mixed evenly to obtain the product.

[0050] Test Example 1

[0051] Spf-grade SD rats were randomly divided into blank group, experimental group and model group, 10 rats in each group, and then caged, with 5 experimental rats in each cage, half male and half female, and kept in separate cages. Adaptive feeding for 3 days, no activity restriction, free food and water. After 3 days of adaptive feeding of rats, PCPA (parachlorophenylalanine) suspension 350mg / kg was intraperitoneally injected into the experimental group and model group rats, once a day, for 2 consecutive days. After continuous intraperitoneal injection, the modeling rats have lost their normal circadian rhythm and are active all day and night, indicating that the model has been successfully replicated. The blank group and the model group were gavaged with an equal volume of 10mL of saline, once a day. The experimental group was gavaged with a dose of 1g / kg, the gavage volume was 10mL, once a day, and the administration was continued for 7 days according to the corresponding administration method. Four hours after the last administration, the rats were anesthetized and killed by an overdose of sodium pentobarbital, the brain was quickly removed, the blood was washed with ice saline, and the hypothalamus was quickly separated on an ice tray. The kit was used to detect the 5-HT content in the rat hypothalamus. The specific results are shown in Table 1.

[0052] Table 1 Effect of the composition of Apocynum venetum extract on 5-HT content in rat hypothalamus

[0053] Experimental protocol 5-HT content / ng / mL Blank Group 4.35±0.23 Model Group 3.50±0.14 Example 1 4.36±0.12 Example 2 4.51±0.13 Example 3 4.91±0.32 Comparative Example 1 4.12±0.16 Comparative Example 2 3.98±0.19

[0054] 5-HT, also known as 5-hydroxytryptamine, is an important neurotransmitter, also known as serotonin. It has a wide distribution in the human body and has multiple physiological functions. 5-HT is mainly distributed in the pineal gland and hypothalamus in the brain, and is involved in the regulation of physiological functions such as pain, sleep and body temperature. In the central nervous system, 5-HT is an important neurotransmitter that participates in the regulation of sleep and plays an important role.

[0055] As can be seen from Table 1, compared with the control example 2, the content of 5-HT in the rat hypothalamus is significantly higher in the embodiment, which may be due to the fact that hyperoside and isoquercetin in the apocynum extract will be degraded to varying degrees under high temperature, high humidity or light conditions, and isoquercetin is almost insoluble in water, so the efficacy is poor when not loaded, and the embodiment provides a kind of apocynum extract nanoparticles, using modified zein as a carrier, so the present invention uses amino acids to modify it, so that the conformation of zein changes, thereby causing protein subunit decomposition, improving the solubility of zein, and zein itself has unique self-assembly characteristics, so it can be used as an encapsulation delivery carrier, which helps to encapsulate bioactive substances with hydrophilicity, hydrophobicity and amphiphilicity. The grafted zein has better solubility, so the encapsulation rate and dispersibility are improved, and the protection of the apocynum extract and the role of the effective ingredients are also stronger. However, since the isoelectric point of zein is 6.2-6.8, the colloidal stability of the prepared nanoparticles in aqueous medium is poor, and the single prepared zein drug-loaded particles have the disadvantages of low encapsulation rate, poor water solubility, poor stability and poor redispersibility after freeze-drying, so the sleep-aiding effect in Control Example 2 is not as good as that in Example 1. Example 3 Compared with Example 1, zein is modified by γ-aminobutyric acid, and the extract of Apocynum venetum is encapsulated in the modified zein, so that when it is released, it can be easily combined with the γ-aminobutyric acid grafted on the surface of zein, and hyperoside and isoquercetin may enhance the effect of GABA after combining with GABA, thereby playing a more effective role in tranquilizing the nerves and helping sleep, so the content of 5-HT in the hypothalamus of rats in Example 3 is the highest.

[0056] Test Example 2

[0057] Spf-grade SD rats were randomly divided into blank group, experimental group and model group, 10 rats in each group, and then caged, with 5 experimental rats in each cage, half male and half female, and kept in separate cages. Adaptive feeding for 3 days, no activity restriction, free food and water. After 3 days of adaptive feeding of rats, PCPA (para-chlorophenylalanine) suspension 350mg / kg was intraperitoneally injected into the experimental group and model group rats, once a day, for 2 consecutive days. After continuous intraperitoneal injection, the modeling rats have lost their normal circadian rhythm and are active all day and night, indicating that the model has been successfully replicated. The blank group and the model group were gavaged with an equal volume of 10mL of normal saline, once a day. The experimental group was gavaged with a dose of 1g / kg, the gavage volume was 10mL, once a day, and the medication was continued for 7 days according to the corresponding administration method. Each group of rats was intraperitoneally injected with 30mg / kg, 35mg / kg, 40mg / kg, 45mg / kg, and 55mg / kg of sodium pentobarbital, and the sleep of the rats was observed. The dose at which 90-100% of rats fall asleep within 10-15 minutes and can wake up within 1 hour is considered as the suprathreshold dose. Rats were fasted for 8 hours before the experiment but not water. 30 minutes after the last administration, the blank group, model group, and experimental group were intraperitoneally injected with a suprathreshold dose of sodium pentobarbital (40 mg / kg). The sleep latency and sleep time of rats with suprathreshold doses were observed and recorded (the disappearance of the righting reflex of rats for 1 minute after administration was taken as the indicator of falling asleep, i.e., the sleep latency of rats, and the time from the disappearance of the righting reflex to the recovery was taken as the sleep time). The specific results are shown in Table 2.

[0058] Table 2 Effect of the composition of Apocynum venetum extract on the hypnotic effect of sodium pentobarbital above the threshold dose in rats

[0059] Experimental protocol Sleep latency / min Sleep time / min Blank Group 3.65±0.51 66.58±3.34 Model Group 6.23±0.68 23.35±1.77 Example 1 3.15±0.50 75.56±3.55 Example 2 3.14±0.48 75.34±3.67 Example 3 2.24±0.32 86.78±3.90 Comparative Example 1 3.38±0.55 66.45±3.02 Comparative Example 2 3.50±0.53 62.31±3.66

[0060] Sodium pentobarbital can produce a wide range of inhibitory effects on the central nervous system. Depending on the dosage, it can produce sedative, hypnotic and anticonvulsant effects. In large doses, it can also produce anesthetic effects. The hypnotic effect of the drug can be evaluated by observing the behavioral responses of rats at suprathreshold doses of sodium pentobarbital. In the experiment, the effect of the drug on sleep was evaluated by recording sleep latency and sleep time.

[0061] As can be seen from Table 2, the Apocynum venetum extract prepared by the present invention has a good tranquilizing effect and helps sleep, enhances the hypnotic effect of sodium pentobarbital, effectively shortens the sleep latency of rats, and prolongs the sleep time of rats. However, Example 3 has the best sleep-aiding effect on rats, which may be due to the modification of zein by γ-aminobutyric acid in Example 3. By encapsulating the Apocynum venetum extract in the modified zein, it can be easily combined with the γ-aminobutyric acid grafted on the surface of zein when it is released. After hyperoside and isoquercetin are combined with GABA, the effect of GABA may be enhanced, thereby playing a more effective tranquilizing effect.

[0062] Test Example 3

[0063] According to GB 15193.1-2014 National Food Safety Standard Food Safety Toxicology Evaluation Procedure, the first phase acute toxicity test and the second phase genetic toxicity test, the traditional teratogenicity test, and the 90-day oral toxicity test of the present invention's Apocynum venetum extract composition were carried out. The results showed that the acute toxicity test results were non-toxic; the bone marrow cell micronucleus test, the mouse sperm teratogenicity test, and the 90-day oral toxicity test were all negative. The toxicology test results provide a basis for the edible safety of the present invention.

[0064] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A composition of apocynum venetum extract, characterized in that: The invention comprises the following raw materials in parts by weight: 30-50 parts of sour jujube seeds, 20-30 parts of lilies, 5-10 parts of modified zein nanoparticles loaded with apocynum venetum extract, 4-6 parts of theanine and 1-3 parts of vitamin B6.

2. The composition of the Apocynum venetum extract according to claim 1, characterized in that The method for preparing modified zein nanoparticles loaded with apocynum venetum extract comprises the following steps: S1, dissolving zein in an ethanol aqueous solution, adding amino acids, and then adding 4-dimethylaminopyridine and triethylamine, dialyzing and freeze-drying after the reaction to obtain modified zein; S2. The modified zein and the Apocynum venetum extract are subjected to an anti-solvent precipitation method to obtain modified zein nanoparticles loaded with the Apocynum venetum extract.

3. The composition of the Apocynum venetum extract according to claim 2, characterized in that: The method for preparing the modified zein nanoparticles loaded with apocynum venetum extract comprises the following steps: S1, dissolving zein in a 70-80 wt% ethanol aqueous solution, adding amino acids, and then adding 4-dimethylaminopyridine and triethylamine, stirring at 20-30° C. for 20-30 h, dialyzing for 2-3 days, and freeze-drying to obtain modified zein; S2. Dissolving the modified zein and the apocynum venetum extract in a 70-80 wt% ethanol aqueous solution to obtain an organic phase, adding the organic phase to 9-10 times the volume of water under stirring, standing after stirring, and freeze-drying to obtain modified zein nanoparticles loaded with the apocynum venetum extract.

4. The composition of the Apocynum venetum extract according to claim 3, characterized in that: The mass ratio of the zein to the ethanol aqueous solution is 1:50-100.

5. The composition of the Apocynum venetum extract according to claim 3, characterized in that: The mass ratio of the zein to the amino acid, 4-dimethylaminopyridine and triethylamine is 1:0.1-1:0.01-0.2:0.1-0.

5.

6. The composition of the Apocynum venetum extract according to claim 3, characterized in that: The dialyzed molecular weight was <5000Da.

7. The composition of the Apocynum venetum extract according to claim 3, characterized in that: The amino acid is one of lysine, γ-aminobutyric acid and leucine.

8. The composition of the Apocynum venetum extract according to claim 3, characterized in that: The mass ratio of the modified zein to the apocynum venetum extract and the ethanol aqueous solution is 20-30:1:100-500.

9. The method for preparing the apocynum venetum extract composition according to any one of claims 1 to 8, characterized in that: The steps include: The spiny jujube seeds and lily are crushed and mixed with modified zein nanoparticles loaded with apocynum venetum extract, theanine and vitamin B6 until evenly mixed to obtain the product.

10. Use of the Apocynum venetum extract composition according to any one of claims 1 to 8 in a product for calming nerves and aiding sleep, characterized in that: The tranquilizing and sleeping-aiding product is a probiotic powder or a drink.