Hypoallergenic bovine colostrum compound for pets and preparation method of hypoallergenic bovine colostrum compound

Abstract

The invention relates to a hypoallergenic bovine colostrum compound for pets. The milk powder comprises the following components in percentage by mass: 70%-95% of bovine colostrum, 10%-15% of protein hydrolysate, 1%-7% of a vitamin and trace element premix, 1%-8% of fructo-oligosaccharide, 0.01%-0.15% of sodium benzoate, 0.005%-0.015% of disodium ethylene diamine tetraacetate, 0.5%-1.5% of lactobacillus acidophilus, 0.1%-0.3% of lecithin, 0.2%-0.5% of glutamine, 0.1%-0.3% of beta-glucan, 0.3%-0.6% of arginine, 0.1%-0.3% of nano-hydroxyapatite, 0.05%-0.15% of a rosemary extract, 0.05%-0.1% of vitamin C and 1%-3% of a yolk immunoglobulin IgY and enzyme mixture. The bovine coloctrum provides immune support, the fructooligosaccharide regulates intestinal flora, the vitamin and trace element premix supplements trace elements and the like, and after enzymolysis and emulsification, nutrition is easy to absorb, the physiological state is rapidly regulated, and stress is relieved. The disodium ethylene diamine tetraacetate stabilizes the nutritional ingredients in the form of the nutritional paste, and can be effectively absorbed by the young pets in a stress state, so that the influence on the nutrient intake of the young pets due to the coagulation problem is avoided.

Description

Technical Field

[0001] The present invention relates to the field of pet compound feed, and in particular to a low-allergy bovine colostrum compound for pets and a preparation method thereof. Background Art

[0002] In the field of modern food processing, the allergenicity of milk protein has always been a matter of great concern. In order to reduce the allergenicity of milk protein, researchers have adopted a variety of technical means, among which heating, high-pressure treatment, glycosylation modification and enzymatic hydrolysis modification are the more commonly used milk protein desensitization technologies. These technologies mainly achieve the purpose of reducing allergenicity by destroying or masking the antigenic epitopes of allergens. In terms of the desensitization rate, the desensitization effect of some technologies is not ideal, and the desensitization rate is low. From the flavor point of view, the application of some technologies will change the original flavor of milk, such as producing bitterness, which will have a negative impact on the taste and market acceptance of the product. At the same time, in terms of nutritional value, some desensitization technologies may cause the loss of nutritional value of milk protein. In addition, cost is also an issue that cannot be ignored. The cost of some desensitization technologies is high, which will increase the production and sales costs of products and affect their market competitiveness.

[0003] In terms of animal nutrition and immunity, bovine colostrum and other resources are relatively scarce in the immune nutrition combination of newborn animals and critically ill animals. At present, although there are some methods to deploy nutritional immunity programs according to the physiological state of animals, these methods have many shortcomings. For example, animals have a low absorption rate of nutrients, which means that even if a nutritional program is provided, the nutrients that animals can actually absorb and utilize are limited. Moreover, in terms of the effect of nutritional supplementation, the current methods are not very ideal, and it is difficult to achieve the expected effects of enhancing animal immunity. Summary of the invention

[0004] The purpose of the present invention is to provide a low-sensitivity bovine colostrum complex for pets and a preparation method thereof, which solves the problems of poor hyposensitivity effect of the above-mentioned bovine colostrum and low nutrient absorption rate of animals.

[0005] To achieve the above-mentioned object, the present invention provides a low-allergy bovine colostrum complex for pets, the components of which include, by mass percentage, 70% to 95% bovine colostrum, 10% to 15% hydrolyzed protein, 1% to 7% vitamin and trace element premix, 1% to 8% fructooligosaccharide, 0.01% to 0.15% sodium benzoate, 0.005% to 0.015% disodium edetate, 0.5% to 1.5% Lactobacillus acidophilus, 0.1% to 0.3% lecithin, 0.2% to 0.5% glutamine, 0.1% to 0.3% β-glucan, 0.3% to 0.6% arginine, 0.1% to 0.3% nano-hydroxyapatite, 0.05% to 0.15% rosemary extract, 0.05% to 0.1% vitamin C, 1% to 3% egg yolk immunoglobulin IgY and an enzyme mixture.

[0006] Preferably, the vitamin and trace element premix comprises 60% to 70% vitamin A, 8% to 12% vitamin D3, 4% to 5% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.05% to 0.35% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.3% to 0.5% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

[0007] Preferably, the enzyme mixture comprises trypsin, xyloproteinase and pepsin, wherein the ratio of the three is 1:1:0.5.

[0008] Preferably, the pepsin is subjected to an embedding treatment: the pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

[0009] A method for preparing a hypoallergenic bovine colostrum complex for pets, comprising the following steps:

[0010] (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum.

[0011] The reaction pH is 5.3-5.8, the temperature is 48°C-55°C, the reaction time is 120-150 min, and the ratio of trypsin, xyloproteinase and pepsin is 1:1:0.5;

[0012] (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution;

[0013] (3) Vitamin microcapsules were prepared by emulsification-solvent evaporation method, and ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix in microcapsules;

[0014] (4) premixing the vitamin microcapsules prepared in the previous step with other vitamin and trace element premixes, and preparing supplementary granules with starch at a mass ratio of 1:5;

[0015] (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum.

[0016] Among them, the stirring speed is 30 ~40r / min, and the stirring time is 30 ~40min;

[0017] (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum,

[0018] Wherein, the mixing temperature is 42 ~ 45 ° C, the stirring speed is 30 ~ 40r / min, and the stirring time is 40 ~ 45min;

[0019] (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir.

[0020] Wherein, the stirring temperature is 35-40°C, the stirring speed is 20-30r / min, and the stirring time is 20-30min;

[0021] Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum.

[0022] Wherein, the stirring temperature is 30~35°C, the stirring speed is 15~25r / min, and the stirring time is 15~20min;

[0023] (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (7), stirring and mixing, to prepare a bovine colostrum complex;

[0024] (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product.

[0025] Among them, the power of plasma treatment is 100 ~300W, the treatment time is 5 ~10min, the temperature of pasteurization is 72 ~75°C, and the time is 15 ~20s.

[0026] Preferably, the hydrolyzed protein is prepared by the following steps:

[0027] Fresh bovine colostrum is filtered, and then mixed with trypsin and xyloproteinase in a container for reaction. The reaction pH is 6 to 8, the temperature is 45°C to 55°C, and the reaction time is 2 to 4 hours.

[0028] After the reaction is completed, the mixture is heated to 70°C to 80°C for 10 to 15 minutes, and then filtered and centrifuged to obtain hydrolyzed protein;

[0029] Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:100.

[0030] Preferably, the speed and premixing time of the ball mill premixing in step (2) are set according to the following formula:

[0031] when hour, , ,

[0032] when hour, , ,

[0033] when hour, , ,

[0034] Among them, m is the material amount in the ball mill, n is the rotation speed of the ball mill, and t is the premixing time.

[0035] Preferably, the two-stage ultrasonic dispersion in step (2) includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min,

[0036] The parameters of the second stage ultrasonic dispersion are set by the following formula:

[0037] when hour, , , ,

[0038] when hour, , , ,

[0039] when hour, , , ,

[0040] The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2.

[0041] The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

[0042] Preferably, the ratio of said sodium benzoate to said sodium benzoate in step (7) is 5:2;

[0043] The temperature of the sterile water in which the rosemary extract is dissolved is 30-40°C, and the temperature of the sterile water in which the sodium benzoate is dissolved is 20-25°C.

[0044] Preferably, in step (7), the dissolved rosemary extract solution is first added to the second mixed bovine colostrum, with a stirring speed of 100 to 200 r / min and a stirring time of 5 to 10 min;

[0045] Then add the dissolved sodium benzoate solution, stirring at a speed of 300 ~500r / min and for 10 ~15min.

[0046] Beneficial effects of the present invention:

[0047] 1. The present invention works in many aspects. Bovine colostrum provides immune support, fructooligosaccharides regulate intestinal flora, vitamins and trace element premixes supplement trace elements, etc. After enzymatic hydrolysis and emulsification, the nutrients are easily absorbed and the physiological state is quickly adjusted to reduce stress. Disodium EDTA stabilizes the nutrients in the form of nutritional paste and can be effectively absorbed by young pets under stress, avoiding the problem of coagulation that affects the young pets' nutrient intake.

[0048] 2. In the preparation of hydrolyzed protein, the present invention uses multiple enzymes such as trypsin and xyloproteinase, and strictly controls the reaction conditions. These enzymes act on the proteins in bovine colostrum under specific conditions, and can accurately break specific peptide bonds and decompose macromolecular proteins into small molecular peptides and amino acids. This precise hydrolysis process can change the structure of the protein, so that it can cooperate with other enzymes (trypsin, xyloproteinase, pepsin, etc.) in subsequent steps, such as preparing enzyme-killed bovine colostrum, to further modify the protein.

[0049] 3. The vitamins contained in the product of the present invention, such as vitamin B1, vitamin B2, vitamin B9, etc., each play a different physiological function. The preparation of vitamin microcapsules adopts the emulsification-solvent volatilization method, and ethyl cellulose and polyvinyl alcohol are used to encapsulate vitamin B1, vitamin B2, vitamin B9, etc. in microcapsules. This encapsulation method provides a physical barrier for vitamins, preventing the vitamins from being degraded by external factors (such as light, heat, oxygen, etc.) during storage and processing. Microcapsules can control the release rate of vitamins, so that they can be released at the appropriate time and location after ingestion by pets, thereby improving the bioavailability of vitamins. DETAILED DESCRIPTION

[0050] Embodiments of the present invention are described in detail below. The embodiments described below are exemplary and intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0051] Example 1

[0052] A low-allergy bovine colostrum complex for pets, comprising, by mass percentage, 70% bovine colostrum, 10% hydrolyzed protein, 1% vitamin and trace element premix, 1% fructooligosaccharide, 0.01% sodium benzoate, 0.005% disodium edetate, 0.5% Lactobacillus acidophilus, 0.1% lecithin, 0.2% glutamine, 0.1% beta-glucan, 0.3% arginine, 0.1% nano-hydroxyapatite, 0.05% rosemary extract, 0.05% vitamin C, 1% egg yolk immunoglobulin IgY and an enzyme mixture.

[0053] Among them, the vitamin and trace element premix contains 60% vitamin A, 8% vitamin D3, 4% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.05% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.3% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

[0054] The enzyme mixture includes trypsin, xylosidase and pepsin in a ratio of 1:1:0.5.

[0055] Specifically, pepsin is subjected to an embedding treatment: pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

[0056] A method for preparing a hypoallergenic bovine colostrum complex for pets, comprising the following steps:

[0057] (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum.

[0058] The reaction pH was 5.3, the temperature was 48°C, the reaction time was 120 min, and the ratio of trypsin, xyloproteinase, and pepsin was 1:1:0.5;

[0059] Further, the hydrolyzed protein is prepared by the following steps:

[0060] Fresh bovine colostrum was filtered, and the filtered bovine colostrum was mixed with trypsin and xyloproteinase in a container for reaction, with a reaction pH of 6, a temperature of 45°C, and a reaction time of 2h.

[0061] After the reaction is completed, the mixture is heated to 70°C for 10 min, and then filtered and centrifuged to obtain hydrolyzed protein.

[0062] Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:100.

[0063] (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution;

[0064] Specifically, the speed and premixing time of the ball mill premixing are set according to the following formula:

[0065] when hour, , ,

[0066] when hour, , ,

[0067] when hour, , ,

[0068] Among them, m is the amount of material in the ball mill, n is the speed of the ball mill, and t is the premixing time;

[0069] Furthermore, the two-stage ultrasonic dispersion includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min,

[0070] The parameters of the second stage ultrasonic dispersion are set by the following formula:

[0071] when hour, , , ,

[0072] when hour, , , ,

[0073] when hour, , , ,

[0074] The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2.

[0075] The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

[0076] (3) Vitamin microcapsules were prepared by the emulsification-solvent evaporation method. Ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix into microcapsules.

[0077] (4) The vitamin microcapsules prepared in the previous step are premixed with other vitamin and trace element premixes, and then mixed with starch at a mass ratio of 1:5 to prepare supplementary granules.

[0078] (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum.

[0079] Among them, the stirring speed is 30r / min and the stirring time is 30min.

[0080] (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum,

[0081] The mixing temperature was 42 °C, the stirring speed was 30 r / min, and the stirring time was 40 min.

[0082] (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir.

[0083] Wherein, the stirring temperature is 40°C, the stirring speed is 30r / min, and the stirring time is 30min;

[0084] Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum.

[0085] Wherein, the stirring temperature is 35°C, the stirring speed is 25r / min, and the stirring time is 20min;

[0086] (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (7), stirring and mixing, to prepare a bovine colostrum complex;

[0087] Further, the ratio of rosemary extract to sodium benzoate is 5:2, the temperature of sterile water for dissolving rosemary extract is 30°C, and the temperature of sterile water for dissolving sodium benzoate is 20°C;

[0088] Specifically, the dissolved rosemary extract solution is first added to the third mixed bovine colostrum, with a stirring speed of 100 r / min and a stirring time of 5 min; and then the dissolved sodium benzoate solution is added, with a stirring speed of 300 r / min and a stirring time of 10 min.

[0089] (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product.

[0090] The power of plasma treatment was 100 W, the treatment time was 5 min, the temperature of pasteurization was 72 ° C, and the time was 15 s.

[0091] Example 2

[0092] A low-allergy bovine colostrum complex for pets, comprising, by mass percentage, 95% bovine colostrum, 15% hydrolyzed protein, 7% vitamin and trace element premix, 8% fructooligosaccharide, 0.15% sodium benzoate, 0.015% disodium edetate, 1.5% Lactobacillus acidophilus, 0.3% lecithin, 0.5% glutamine, 0.3% β-glucan, 0.6% arginine, 0.3% nano-hydroxyapatite, 0.15% rosemary extract, 0.1% vitamin C, 3% egg yolk immunoglobulin IgY and an enzyme mixture.

[0093] Among them, the vitamin and trace element premix contains 70% vitamin A, 12% vitamin D3, 5% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.35% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.5% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

[0094] The enzyme mixture includes trypsin, xylosidase and pepsin in a ratio of 1:1:0.5.

[0095] Specifically, pepsin is subjected to an embedding treatment: pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

[0096] A method for preparing a hypoallergenic bovine colostrum complex for pets, comprising the following steps:

[0097] (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum.

[0098] The reaction pH was 5.8, the temperature was 55°C, the reaction time was 150 min, and the ratio of trypsin, xyloproteinase, and pepsin was 1:1:0.5;

[0099] Further, the hydrolyzed protein is prepared by the following steps:

[0100] Fresh bovine colostrum was filtered, and the filtered bovine colostrum was mixed with trypsin and xyloproteinase in a container for reaction, with a reaction pH of 8, a temperature of 55°C, and a reaction time of 4 hours.

[0101] After the reaction is completed, the mixture is heated to 80°C for 15 min, and then filtered and centrifuged to obtain hydrolyzed protein.

[0102] Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:100.

[0103] (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution;

[0104] Specifically, the speed and premixing time of the ball mill premixing are set according to the following formula:

[0105] when hour, , ,

[0106] when hour, , ,

[0107] when hour, , ,

[0108] Among them, m is the amount of material in the ball mill, n is the speed of the ball mill, and t is the premixing time;

[0109] Furthermore, the two-stage ultrasonic dispersion includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min,

[0110] The parameters of the second stage ultrasonic dispersion are set by the following formula:

[0111] when hour, , , ,

[0112] when hour, , , ,

[0113] when hour, , , ,

[0114] The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2.

[0115] The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

[0116] (3) Vitamin microcapsules were prepared by the emulsification-solvent evaporation method. Ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix into microcapsules.

[0117] (4) The vitamin microcapsules prepared in the previous step are premixed with other vitamin and trace element premixes, and then mixed with starch at a mass ratio of 1:5 to prepare supplementary granules.

[0118] (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum.

[0119] Among them, the stirring speed is 40r / min and the stirring time is 40min.

[0120] (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum,

[0121] The mixing temperature was 45°C, the stirring speed was 40 r / min, and the stirring time was 45 min.

[0122] (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir.

[0123] Wherein, the stirring temperature is 40°C, the stirring speed is 30r / min, and the stirring time is 30min;

[0124] Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum.

[0125] Wherein, the stirring temperature is 35°C, the stirring speed is 25r / min, and the stirring time is 20min;

[0126] (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (7), stirring and mixing, to prepare a bovine colostrum complex;

[0127] Further, the ratio of rosemary extract to sodium benzoate is 5:2, the temperature of sterile water for dissolving rosemary extract is 40°C, and the temperature of sterile water for dissolving sodium benzoate is 25°C;

[0128] Specifically, the dissolved rosemary extract solution is first added to the third mixed bovine colostrum, with a stirring speed of 200 r / min and a stirring time of 10 min; and then the dissolved sodium benzoate solution is added, with a stirring speed of 500 r / min and a stirring time of 15 min.

[0129] (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product.

[0130] The power of plasma treatment was 300 W, the treatment time was 10 min, the temperature of pasteurization was 75 ° C, and the time was 20 s.

[0131] Example 3

[0132] A low-allergy bovine colostrum complex for pets, comprising, by mass percentage, 85% bovine colostrum, 12% hydrolyzed protein, 4% vitamin and trace element premix, 4% fructooligosaccharide, 0.08% sodium benzoate, 0.01% disodium edetate, 1% Lactobacillus acidophilus, 0.2% lecithin, 0.3% glutamine, 0.2% beta-glucan, 0.4% arginine, 0.2% nano-hydroxyapatite, 0.1% rosemary extract, 0.07% vitamin C, 2% egg yolk immunoglobulin IgY and an enzyme mixture.

[0133] Among them, the vitamin and trace element premix contains 65% vitamin A, 10% vitamin D3, 4% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.2% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.4% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

[0134] The enzyme mixture includes trypsin, xylosidase and pepsin in a ratio of 1:1:0.5.

[0135] Specifically, pepsin is subjected to an embedding treatment: pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

[0136] A method for preparing a hypoallergenic bovine colostrum complex for pets, comprising the following steps:

[0137] (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum.

[0138] The reaction pH was 5.6, the temperature was 51°C, the reaction time was 135 min, and the ratio of trypsin, xyloproteinase, and pepsin was 1:1:0.5;

[0139] Further, the hydrolyzed protein is prepared by the following steps:

[0140] Fresh bovine colostrum was filtered, and the filtered bovine colostrum was mixed with trypsin and xyloproteinase in a container for reaction, with a reaction pH of 7, a temperature of 47°C, and a reaction time of 3 hours.

[0141] After the reaction was completed, the mixture was heated to 75°C for 12 min, and then filtered and centrifuged to obtain hydrolyzed protein.

[0142] Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:100.

[0143] (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution;

[0144] Specifically, the speed and premixing time of the ball mill premixing are set according to the following formula:

[0145] when hour, , ,

[0146] when hour, , ,

[0147] when hour, , ,

[0148] Among them, m is the amount of material in the ball mill, n is the speed of the ball mill, and t is the premixing time;

[0149] Furthermore, the two-stage ultrasonic dispersion includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min,

[0150] The parameters of the second stage ultrasonic dispersion are set by the following formula:

[0151] when hour, , , ,

[0152] when hour, , , ,

[0153] when hour, , , ,

[0154] The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2.

[0155] The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

[0156] (3) Vitamin microcapsules were prepared by the emulsification-solvent evaporation method. Ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix into microcapsules.

[0157] (4) The vitamin microcapsules prepared in the previous step are premixed with other vitamin and trace element premixes, and then mixed with starch at a mass ratio of 1:5 to prepare supplementary granules.

[0158] (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum.

[0159] Among them, the stirring speed is 35r / min and the stirring time is 35min.

[0160] (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum,

[0161] The mixing temperature was 43°C, the stirring speed was 35 r / min, and the stirring time was 42 min.

[0162] (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir.

[0163] Wherein, the stirring temperature is 37°C, the stirring speed is 25r / min, and the stirring time is 25min;

[0164] Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum.

[0165] Wherein, the stirring temperature is 32°C, the stirring speed is 20r / min, and the stirring time is 17min;

[0166] (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (7), stirring and mixing, to prepare a bovine colostrum complex;

[0167] Further, the ratio of rosemary extract to sodium benzoate is 5:2, the temperature of sterile water for dissolving rosemary extract is 35°C, and the temperature of sterile water for dissolving sodium benzoate is 22°C;

[0168] Specifically, the dissolved rosemary extract solution is first added to the third mixed bovine colostrum, with a stirring speed of 150 r / min and a stirring time of 7 min; and then the dissolved sodium benzoate solution is added, with a stirring speed of 400 r / min and a stirring time of 13 min.

[0169] (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product.

[0170] The power of plasma treatment was 200 W, the treatment time was 7 min, the temperature of pasteurization was 73 ° C, and the time was 17 s.

[0171] Example 4

[0172] A low-allergy bovine colostrum complex for pets, comprising, by mass percentage, 90.89% bovine colostrum, 10% hydrolyzed protein, 5% vitamin and trace element premix, 4% fructooligosaccharide, 0.09% sodium benzoate, 0.005% disodium edetate, 0.8% lactobacillus acidophilus, 0.3% lecithin, 0.2% glutamine, 0.2% beta-glucan, 0.5% arginine, 0.28% nano-hydroxyapatite, 0.15% rosemary extract, 0.05%-0.1% vitamin C, 1% egg yolk immunoglobulin IgY and an enzyme mixture.

[0173] Among them, the vitamin and trace element premix contains 70% vitamin A, 8% vitamin D3, 4.5% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.35% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.3% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

[0174] The enzyme mixture includes trypsin, xylosidase and pepsin in a ratio of 1:1:0.5.

[0175] Specifically, pepsin is subjected to an embedding treatment: pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

[0176] A method for preparing a hypoallergenic bovine colostrum complex for pets, comprising the following steps:

[0177] (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum.

[0178] The reaction pH was 5.7, the temperature was 48°C, the reaction time was 135 min, and the ratio of trypsin, xyloproteinase, and pepsin was 1:1:0.5;

[0179] Further, the hydrolyzed protein is prepared by the following steps:

[0180] Fresh bovine colostrum was filtered, and the filtered bovine colostrum was mixed with trypsin and xyloproteinase in a container for reaction, with a reaction pH of 6, a temperature of 45°C, and a reaction time of 3 hours.

[0181] After the reaction was completed, the mixture was heated to 78°C for 12 min, and then filtered and centrifuged to obtain hydrolyzed protein.

[0182] Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:100.

[0183] (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution;

[0184] Specifically, the speed and premixing time of the ball mill premixing are set according to the following formula:

[0185] when hour, , ,

[0186] when hour, , ,

[0187] when hour, , ,

[0188] Among them, m is the amount of material in the ball mill, n is the speed of the ball mill, and t is the premixing time;

[0189] Furthermore, the two-stage ultrasonic dispersion includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min,

[0190] The parameters of the second stage ultrasonic dispersion are set by the following formula:

[0191] when hour, , , ,

[0192] when hour, , , ,

[0193] when hour, , , ,

[0194] The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2.

[0195] The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

[0196] (3) Vitamin microcapsules were prepared by the emulsification-solvent evaporation method. Ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix into microcapsules.

[0197] (4) The vitamin microcapsules prepared in the previous step are premixed with other vitamin and trace element premixes, and then mixed with starch at a mass ratio of 1:5 to prepare supplementary granules.

[0198] (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum.

[0199] The stirring speed was 35 rpm and the stirring time was 30 min.

[0200] (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum,

[0201] The mixing temperature was 42°C, the stirring speed was 35 rpm, and the stirring time was 45 min.

[0202] (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir.

[0203] Wherein, the stirring temperature is 35°C, the stirring speed is 25r / min, and the stirring time is 25min;

[0204] Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum.

[0205] The stirring temperature was 30°C, the stirring speed was 20 r / min, and the stirring time was 15 min.

[0206] (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (6), stirring and mixing, to prepare a bovine colostrum complex;

[0207] Further, the ratio of rosemary extract to sodium benzoate is 5:3, the temperature of sterile water for dissolving rosemary extract is 38°C, and the temperature of sterile water for dissolving sodium benzoate is 25°C;

[0208] Specifically, the dissolved rosemary extract solution is first added to the second mixed bovine colostrum, with a stirring speed of 120 r / min and a stirring time of 10 min; and then the dissolved sodium benzoate solution is added, with a stirring speed of 3800 r / min and a stirring time of 15 min.

[0209] (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product.

[0210] The power of plasma treatment was 220 W, the treatment time was 8 min, the temperature of pasteurization was 75 ° C, and the time was 18 s.

[0211] This hypoallergenic bovine colostrum complex nutritional formula works in many ways. Bovine colostrum provides immune support, fructooligosaccharides regulate intestinal flora, vitamins and trace element premixes supplement trace elements, etc. After enzymatic hydrolysis and emulsification, the nutrients are easily absorbed and the physiological state is quickly adjusted to reduce stress. Disodium ethylenediaminetetraacetic acid (EDTA-Na2) stabilizes the nutrients in the form of a nutritional paste and can be effectively absorbed by young pets even under stress, avoiding the problem of coagulation that affects the young pets' nutritional intake.

[0212] Bovine colostrum is a precious raw material rich in bioactive ingredients such as protein, yolk immunoglobulin and growth factors. In the present invention, these active ingredients can be effectively released through a precisely controlled enzymatic hydrolysis process, thereby improving their bioavailability and functionality. Enzymatic hydrolysis under a specific temperature and pH environment can maximize the activity and stability of the enzyme, thereby improving reaction efficiency and product quality. This method uses the combined use of trypsin and papain. Trypsin mainly acts on the peptide bonds next to the basic amino acids in the protein, while papain is more inclined to break the peptide bonds between aromatic or hydrophobic amino acid residues. This combined enzymatic hydrolysis method can more comprehensively degrade the protein in bovine colostrum, increase the degree of hydrolysis, and release more functional peptides. Through enzymatic hydrolysis, nutrients become easier to absorb, which is particularly suitable for nutritional supplementation of young pets after surgery, and helps mother pets accelerate wound healing and restore physiological functions.

[0213] Furthermore, the addition ratio of trypsin:papain:pepsin is 2:1:0.5. This ratio is based on the synergistic decomposition of proteins, which can more efficiently break down proteins into small molecular peptides and significantly improve the digestibility.

[0214] The vitamin and trace element premix of the present invention aims to supplement the micronutrient deficiency that may exist in bovine colostrum, meet the needs of young pets for essential vitamins and trace elements with precise dosage, and avoid the toxicity risk that may be caused by excessive intake. Through the carefully formulated ratio, the present invention ensures that young pets can take in an appropriate amount of nutrients, which complement the nutritional components of bovine colostrum. The nutritional components of the present invention maximize their benefits through synergy: for example, vitamin D3 can effectively promote the absorption of calcium and phosphorus, while nano-hydroxyapatite is a high-quality source of calcium and phosphorus. The combined effect of the two helps to maintain the bone health of pets. The combination of fructooligosaccharides and Lactobacillus acidophilus is also carefully designed. Fructooligosaccharides provide nourishment for Lactobacillus acidophilus, while Lactobacillus acidophilus enhances the prebiotic effect of fructooligosaccharides by producing an acidic environment, jointly optimizes the intestinal flora structure, and improves intestinal health. Through a comprehensive nutritional strategy, comprehensive and balanced nutritional support is provided for young pets.

[0215] In addition, although fructooligosaccharides, as a prebiotic, cannot be directly digested and absorbed by young pets, they can be used by the beneficial bacteria in the intestines. Under the nourishment of fructooligosaccharides, these beneficial bacteria are able to proliferate in large quantities, effectively competing with and inhibiting the colonization and growth of harmful bacteria (such as Escherichia coli, Salmonella, etc.) in the intestines, thereby optimizing the intestinal microecological environment of young pets. The increased beneficial bacteria help maintain the integrity of the intestinal mucosa, build a physical and biochemical barrier, prevent the invasion of pathogens, and reduce the risk of intestinal infection. At the same time, these beneficial bacteria can also promote the development of the intestinal mucosal immune system and enhance the overall immune capacity of young pets. As part of the beneficial bacteria, Lactobacillus acidophilus plays an important role in maintaining the balance of intestinal microecology, enhancing intestinal immunity, and inhibiting the growth of harmful bacteria.

[0216] Sodium benzoate can effectively inhibit the growth of a variety of microorganisms, including bacteria, molds and yeasts, at a lower concentration, thereby preventing the product from being contaminated by microorganisms during storage and transportation and extending the shelf life of the product. Rosemary extract not only has antioxidant properties, but also has antibacterial functions, which can inhibit the reproduction of a variety of microorganisms such as bacteria and fungi. When sodium benzoate and rosemary extract are used in a ratio of 5:3, they can broaden the antibacterial spectrum, enhance the inhibitory effect on various microorganisms, and further improve the antiseptic effect of the product. The antioxidant properties of rosemary extract help prevent oxidation reactions such as rancidity of product ingredients such as oils and fading of pigments. At the same time, sodium benzoate reduces the impact of microbial metabolites on the chemical stability of the product by inhibiting the growth of microorganisms. In terms of safety, rosemary extract, as a natural plant ingredient, is more popular with consumers. Although sodium benzoate is a chemically synthesized preservative, its reasonable use in combination with rosemary extract can not only play its effective antiseptic role, but also meet consumers' pursuit of the natural properties of the product, ensuring product safety while taking into account consumers' health and preferences.

[0217] As an efficient metal ion chelator, disodium ethylenediaminetetraacetate (EDTA-Na2) can combine with metal ions such as calcium, magnesium, and iron in the solution. This property can prevent metal ions from inducing oxidation, precipitation or other chemical reactions of nutrients, thereby ensuring the stability of nutrients. However, in dairy products, there is a problem when EDTA-Na2 chelates metal ions: the metal ions in dairy products are helpful in maintaining the protein structure. When these metal ions are chelated by EDTA-Na2, the stability of the protein may be affected, and aggregation and precipitation phenomena may occur, which eventually leads to coagulation. In response to this problem, the technical solution adopts the form of a nutritional paste. During the production process, the mixture with EDTA-Na2 added is subjected to ball milling premixing and ultrasonic dispersion treatment. In this way, not only can the advantages of EDTA-Na2 in stabilizing nutrients be fully utilized, but also fat globules and proteins can be evenly distributed in the complex, which helps to maintain the stability of the product and prevent coagulation due to protein aggregation. In addition, the paste-like nutritional compounds are relatively less subject to vibration and impact during transportation and storage, which helps to reduce physical damage to the protein structure, thereby further reducing the risk of coagulation.

[0218] Nano-hydroxyapatite (n-HAP) has good biocompatibility and ion exchange capacity. It can work synergistically with EDTA-Na2 to improve the chelation efficiency of metal ions. Specifically, the calcium ions on the surface of n-HAP can be exchanged with metal ions in the solution, and then these exchanged metal ions will be chelated by EDTA-Na2. In this way, it can more effectively prevent metal ions from causing oxidation, precipitation or chemical reactions of nutrients, ensuring that the nutrients always remain stable.

[0219] The addition of hydrolyzed protein is intended to solve the protein allergy problem that pets may encounter. Pets are allergic to intact proteins because the immune system mistakenly identifies these proteins as harmful foreign substances. Through the hydrolysis process, large molecular proteins are converted into small molecular peptides. The change in their structure reduces the risk of being recognized by the immune system, thereby reducing the occurrence of allergic reactions. The addition of hydrolyzed protein can not only replace intact proteins that may cause allergies, but also provide a high-quality source of protein. Hydrolyzed protein technology breaks down large molecular proteins into small molecular peptides that are easier to digest and absorb, which not only reduces the risk of allergies, but also improves the absorption efficiency of protein by pets.

[0220] In addition, beta-glucan has the function of regulating the immune system. Lecithin has a positive effect on the liver health, hair gloss and nervous system function of pets. It also improves the digestion and absorption of fat by pets and provides essential fatty acids for pets. Arginine plays an important role in the physiological processes of pets such as urea cycle and immune regulation. Vitamin C, as an antioxidant, participates in many physiological activities in pets, including collagen synthesis and immune regulation. Glutamine helps maintain the integrity of the pet's intestinal mucosa, enhances the intestinal barrier function, and reduces the risk of intestinal infection. When pets are in illness, surgery or other stressful conditions, glutamine supplementation can promote the recovery of pets.

[0221] As a key immune enhancer, egg yolk immunoglobulin IgY is essential for improving the immune capacity of pets. It is especially suitable for pets whose immune systems are not yet mature or have declined, such as puppies, kittens and elderly pets. The supplementation of IgY can provide them with an additional immune barrier, thereby reducing the possibility of illness. In addition, the hypoallergenic properties of IgY make it an ideal choice for pets allergic to specific proteins, providing a safer and optimized solution for pets with a history of food allergies. With its high affinity and precise specificity for specific pathogens, IgY can effectively provide specialized immune protection against diseases such as common intestinal infections in pets.

[0222] In the pet food preparation process (especially the preparation of refined high-end pet food), the present invention integrates massive data and advanced intelligent technology to achieve precise parameter optimization and real-time adjustment of the nanocomposite mixed liquid construction process. The process can intelligently adapt to the characteristics of different raw material batches, equipment operating status and strict product quality standards, thereby intelligently adjusting the operating parameters of ball milling and ultrasonic equipment, ensuring the stability, efficiency and high quality of nanocomposite mixed liquid preparation. In the premixing stage of n-HAP and disodium ethylenediaminetetraacetic acid, the present invention adopts ball milling technology, and intelligently adjusts the speed n and premixing time t of the ball mill according to the material amount m to adjust the equipment parameters in real time. This process design not only takes into account the influence of material quantity changes on particle interaction, but also reflects the effective control of production costs: for less material, by reducing the speed and shortening the premixing time, the mixing effect is guaranteed, and the waste of energy and excessive wear of equipment are avoided; for a large amount of material, by increasing the speed and extending the premixing time, the uniformity of mixing is ensured, the agglomeration phenomenon is effectively prevented, and the production efficiency and cost are balanced.

[0223] Specifically, the present invention can achieve intelligent mixing, so that in the preparation stage of the nanocomposite mixed liquid, the uniformity of the mixing can be ensured, and the performance of the equipment can be well controlled to avoid excessive energy consumption. This means that when the uniformity is low, the power needs to be significantly increased with the uniformity to better promote dispersion. ; and when In this uniformity range, the material has a certain uniformity and does not need to increase the power as much as when the uniformity is very low. Therefore, the coefficient of the (U1-0.5) part is 0.2, and there is a fixed additional value of 0.3, its coefficient becomes smaller, and the fixed additional value takes into account the overall energy demand, that is, ; Finally At this time, the uniformity is already high, and only a small increase in power is needed to maintain or further improve the uniformity. The present invention establishes a clear calculation relationship between the key parameters of ultrasonic dispersion (power, frequency, time) and the uniformity of the mixture, taking into account the different characteristics of the ultrasonic energy requirements of materials at different uniformities, making the ultrasonic dispersion process more scientific, reasonable and efficient.

[0224] After premixing, the mixture is combined with a small amount of bovine colostrum for multi-stage ultrasonic dispersion. The first stage uses the cavitation effect of ultrasound to achieve initial uniform dispersion of the mixture; the second stage adjusts the parameters based on the results of the first stage to further improve the uniformity of the mixture. The ultrasonic equipment is equipped with an artificial intelligence control system that can automatically adjust the ultrasonic power, frequency and processing time based on real-time monitoring of the uniformity of the mixed system and other data, thereby optimizing the ultrasonic treatment process, improving the stability and uniformity of the nanocomposite system, and effectively reducing agglomeration.

[0225] Furthermore, vitamins B1, B2, and B9 are first made into microcapsules, which helps protect the vitamins from the external environment and can also control the release rate of the vitamins so that they can be slowly released as needed in the gastrointestinal tract of the pet, thereby improving the bioavailability of the vitamins. The components made into microcapsules are then premixed with other trace components and carriers to form small particles. Starch is used as a carrier to make the vitamin and trace element premixes better mixed and maintain stability during subsequent processing and storage.

[0226] Specifically, the preparation method of microcapsules of vitamins B1, B2, and B9 can be as follows: first, ethyl cellulose and polyvinyl alcohol are added to the above-mentioned premix (vitamin B1, B2, B9) in a certain ratio (a ratio of 1:1 to 1:3 is used according to actual conditions), and then an appropriate amount of organic solvent (dichloromethane) is added to form a uniform oil phase solution. Then, an appropriate amount of water is prepared as the aqueous phase, and the oil phase is slowly added to the aqueous phase, and a high-speed stirrer or homogenizer is used for stirring, and stirring is continued until the oil phase forms a uniform emulsion in the aqueous phase. Then, the formed emulsion is transferred to a suitable container, and a rotary evaporator is used to set a suitable temperature and speed to gradually volatilize the organic solvent, and the oil droplets in the emulsion gradually solidify to form microcapsules. Finally, the obtained microcapsules will adhere to the container wall or form a precipitate. The microcapsules are collected by a suitable method, such as centrifugal separation or filtration, and the collected microcapsules are dried to obtain a dried vitamin microcapsule product.

[0227] The present invention adopts processes such as ball milling premixing, ultrasonic dispersion and multi-step stirring and mixing to ensure the uniform distribution of the components in the product, thereby ensuring the stability of the product quality and enabling the components to work together effectively. After the first stage of ultrasonic dispersion is completed, the present invention uses online monitoring equipment, including but not limited to an optical microscope combined with image analysis software, to detect the uniformity of particle distribution in the mixed system. Through data analysis, the ultrasonic parameters of the second stage can be accurately calculated, reducing energy waste caused by improper parameter settings and improving the texture and stability of the nano-composite mixed liquid. This method not only improves the overall stability and nutritional protection effect of the product, but also effectively solves the agglomeration problem of nano-hydroxyapatite (n-HAP) particles, while optimizing the efficiency of energy use.

[0228] The final plasma treatment is used for sterilization and disinfection, and a short pasteurization is selected as a supplementary sterilization measure. As a non-thermal sterilization technology, plasma treatment has little impact on nutritional components and taste while ensuring the sterilization effect. Supplementary pasteurization can further ensure the thoroughness of sterilization and improve product safety.

[0229] A trial evaluating the nutritional enhancement effect in cats during postoperative recovery

[0230] Twelve cats that underwent pathological ovariohysterectomy were randomly divided into a control group and an experimental group, with 6 cats in each group. Specific experimental animal information is shown in Table 1.1.

[0231]

[0232] Test materials: the above-mentioned nutritional complex (such as the complex prepared in Example 4, hereinafter referred to as nutritional paste), a certain brand of complete adult cat food (hereinafter referred to as basic daily diet), a blood routine tester, a blood biochemical tester, a weighing scale, a thermometer, and a stethoscope.

[0233] Feeding method: The experimental group was fed with nutritional paste + basic diet, and the control group was fed with basic diet. The specific feeding plan is shown in Table 1.2.

[0234]

[0235] DER calculation method follows DER=K*RER, where K is the life stage factor and RER=70*BW 0.75 kCal, see Table 1.3 for specific calculation method.

[0236]

[0237] Physical condition evaluation

[0238] 2.1 Experimental purpose: According to the clinical cat physical condition scoring standard, the physical condition of the experimental animals was evaluated before surgery, on the 4th day after surgery and when the stitches were removed. The mental state, weight, basic physical signs (heart rate, respiration and body temperature) and BCS of the experimental animals were observed and the results were recorded. The effect of the nutritional paste on the physical condition of postpartum cats was detected by observing the changes in indicators.

[0239] 2.2 Test steps

[0240] 1) The experimental animals were examined before surgery, on the 4th day after surgery, and at the time of suture removal.

[0241] 2) Use a scale to record weight, use an electronic thermometer to measure rectal temperature, and use a stethoscope to auscultate heart rate. Assess mental status and BCS according to the mental status assessment form and BCS assessment form. See Tables 2.1 and 2.2 for specific standards.

[0242]

[0243]

[0244] Stool evaluation

[0245] 3.1 Test purpose: The fecal status can better reflect the intestinal status of cats, the intestinal stress response to food and the digestibility of food. The fecal status changes of the test cats were recorded according to the fecal scoring standard, and the effect of the nutritional paste on the intestinal status and intestinal flora of cats was tested by observing the fecal changes.

[0246] 3.2 Test steps

[0247] 1) Observe the cat’s defecation and feces.

[0248] 2) Record the cat’s fecal status according to the fecal scoring standards. See Table 3.1 for specific standards.

[0249]

[0250] Changes of blood routine and blood biochemical indicators

[0251] 4.1 Purpose of the experiment: Routine blood test and blood biochemistry are the most common and basic hematological examination methods in clinical practice. They mainly examine blood cells and are common auxiliary examination methods for clinical assessment of animal conditions. Routine blood test and blood biochemistry examination of animals before surgery can better clarify the animal's condition and judge the risk level and precautions of surgery. Routine blood test and blood biochemistry examination of animals after surgery can directly reflect the degree of animal recovery. This experiment tested the effect of nutritional paste on the postoperative recovery of experimental animals by performing routine blood test and blood biochemistry examination on experimental animals.

[0252] 4.2 Test steps

[0253] 1) Before the operation, draw blood from the experimental animals, test blood routine and blood biochemistry, and record various indicators that are most related to the disease

[0254] The reference range of normal values ​​for each indicator is shown in Table 4.1.

[0255] On the 4th day after surgery, blood was drawn from the experimental animals to test routine blood tests and blood biochemistry, and three indicators that are most closely related to the disease were recorded. The normal reference range of each indicator is shown in Table 4.1.

[0256]

[0257] Experimental evaluation of nutritional enhancement in cats during the postpartum recovery period

[0258] Experimental animals and materials

[0259] 5.1 Experimental Animal Information Six cats of the same breed, similar age and similar litter size were randomly divided into a control group and an experimental group, with 3 cats in each group. Specific experimental animal information is shown in Table 5.1 below.

[0260]

[0261] Physical assessment

[0262] 6.1 Purpose of the experiment: According to the clinical cat body condition scoring standard, the cats were evaluated every 3 days to observe their mental state, weight, basic physical signs (body temperature, respiration, heart rate) and BCS, and the results were recorded. The effect of the nutritional paste on the body condition of postpartum cats was tested by observing the changes in indicators.

[0263] 3.2 Test steps

[0264] 3) The test cats were examined every 3 days.

[0265] All cats were examined at 9-10 am on an empty stomach.

[0266] Use a scale to record body weight, use an electronic thermometer to measure rectal temperature, and use a stethoscope to auscultate breathing and heart rate. Use the mental status assessment form and BCS assessment form to assess body weight and BCS. See Tables 2.1 and 2.2 above for specific standards.

[0267] Stool evaluation

[0268] 7.1 Test Purpose: The fecal status can better reflect the intestinal status of cats, the intestinal stress response to food and the digestibility of food. The fecal status changes of the test cats were recorded according to the fecal scoring standard, and the effect of the nutritional paste on the intestinal status and intestinal flora of cats was tested by observing the fecal changes.

[0269] 7.2 Test procedures

[0270] 1) Observe the cat’s defecation and feces.

[0271] 2) Record the cat’s fecal status according to the fecal scoring standards. See Table 3.1 for specific standards.

[0272] Milk production assessment

[0273] 8.1 Purpose of the experiment: Cats with adequate nutrition will secrete sufficient milk to feed their kittens after giving birth, while malnourished mother cats will have a greatly reduced milk production and will lose weight. This experiment will test the nutritional supplementation effect of the nutritional paste by observing whether the mother cats produce enough milk.

[0274] 8.2 Test procedures

[0275] 3) Observe the breast fullness of the test cats and the condition of the kittens at 9-10 am every day.

[0276] Observe the kitten's condition throughout the day.

[0277] Record the changes in the mother cat's breasts and kittens' condition. See Table 8.1 for specific reference standards.

[0278]

[0279] Test results

[0280] 1. Results of the test evaluating the nutritional enhancement effect during the postoperative recovery period of cats

[0281] 1. Results of physical condition assessment test: Before the operation, the experimental animals were mostly depressed, with poor appetite, and most of them had elevated body temperature. Other physical signs were normal, among which No. 1, 10, and 11 had more serious symptoms. During the experiment, the mental state of the animals in the experimental group recovered well, the body temperature returned to the normal range, and the weight change was small. No. 4 gained 0.1kg, and the weight of other animals did not change significantly, and BCS did not change significantly. The mental state of the animals in the control group recovered well, among which No. 8 was still not active during the postoperative review. The weight of the animals in the control group did not change significantly, and No. 7 lost weight by 0.1kg, and BCS did not change significantly. For specific changes, see Table 1 Changes in animal physical condition.

[0282]

[0283] 2. Results of stool evaluation test: All experimental animals had no abnormal stool before surgery. During the test, the overall stool score of the experimental group was relatively stable, and only No. 2 had soft stool and then recovered. The overall stool score of the control group changed greatly, and 4 experimental animals had soft stool (No. 7, No. 8, No. 9, and No. 10), and No. 7 had not recovered at the end of the test. The other experimental animals were normal and stable. See Table 2 for specific changes.

[0284]

[0285] 3. Results of the test on changes in blood routine and blood biochemical indices: The white blood cells, lymphocytes, and neutrophils in the blood routine indices of the experimental animals before surgery showed changes to varying degrees, while the red blood cells did not change significantly. The albumin, globulin, and alkaline phosphatase in the blood biochemical indices showed certain changes. After surgery, the blood routine and blood biochemical indices of the experimental group animals except for No. 4 and No. 5 returned to the normal range. The indicators of the control group animals except No. 7 and No. 12 returned to the normal range, and the indicators of the other experimental animals improved but did not decrease or increase to the normal range. See Table 3 for specific changes.

[0286]

[0287] 2. Results of the test evaluating the nutritional enhancement effect during the postpartum recovery period of cats

[0288] 1. Results of the physical condition assessment test During the test, all the test cats showed no abnormal mental state and basic physical signs, and the BCS score did not change significantly. In terms of weight, the test group gained weight, with an average increase of 0.53kg, while the control group did not gain weight overall and lost weight, with an average weight loss of 0.03kg. (No. 1 gained 0.2kg, No. 2 gained 0.7kg, No. 3 gained 0.7kg, No. 4 lost 0.2kg, No. 5 lost 0.3kg, and No. 6 gained 0.4kg). See Table 4 for specific changes.

[0289]

[0290] 2. Fecal evaluation test results: Compared with the control group, cat No. 2 in the test group showed one change, and cat No. 5 in the control group showed two changes. The other test cats showed normal and stable performance. See Table 5 for specific changes.

[0291]

[0292] 3. Milk production evaluation test results: The overall milk production of the test group was sufficient, and the kittens were in good condition. Cat No. 6 in the control group showed mild deficiency, and the kittens occasionally screamed. See Table 6 for specific performance.

[0293]

[0294] From the above data, it can be seen that the present invention has a significant effect on improving the immunity and survival rate of newborn pets, enhancing the effect of vaccines for young pets, regulating the balance of intestinal flora, assisting postoperative recovery, improving stress response, etc. From immune enhancement (the synergistic effect of egg yolk immunoglobulin and other components in bovine colostrum) to intestinal health (fructooligosaccharides regulate intestinal flora, Lactobacillus acidophilus enhances digestive function, etc.), to growth and development (growth factors, vitamins and trace elements in bovine colostrum, etc.) and product stability (disodium ethylenediaminetetraacetic acid stabilizes nutrients, sodium benzoate preserves, etc.), the present invention can fully meet the diverse needs of young pets in different physiological states and postoperative and postpartum recovery of mother pets.

[0295] What is disclosed above is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiments and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. A hypoallergenic bovine colostrum complex for pets, characterized in that: Its components include, by mass percentage, 70% to 95% bovine colostrum, 10% to 15% hydrolyzed protein, 1% to 7% vitamin and trace element premix, 1% to 8% fructooligosaccharide, 0.01% to 0.15% sodium benzoate, 0.005% to 0.015% disodium edetate, 0.5% to 1.5% lactobacillus acidophilus, 0.1% to 0.3% lecithin, 0.2% to 0.5% glutamine, 0.1% to 0.3% beta-glucan, 0.3% to 0.6% arginine, 0.1% to 0.3% nano-hydroxyapatite, 0.05% to 0.15% rosemary extract, 0.05% to 0.1% vitamin C, 1% to 3% egg yolk immunoglobulin IgY and enzyme mixture.

2. The hypoallergenic bovine colostrum complex for pets according to claim 1, characterized in that: The vitamin and trace element premix comprises 60% to 70% vitamin A, 8% to 12% vitamin D3, 4% to 5% vitamin E, 0.04% vitamin B1, 0.02% vitamin, 0.16% vitamin B3, 0.02% vitamin B5, 0.02% vitamin B6, 0.005% vitamin B7, 0.004% vitamin B9, 0.002% vitamin B12, 0.05% to 0.35% trace element iron, 0.021% trace element copper, 0.03% trace element manganese, 0.3% to 0.5% trace element zinc, 0.004% trace element iodine, 0.0001% trace element selenium and 0.0007% taurine.

3. The hypoallergenic bovine colostrum complex for pets according to claim 1, characterized in that: The enzyme mixture includes trypsin, xyloproteinase and pepsin, wherein the ratio of the three is 1:1:0.

5.

4. The hypoallergenic bovine colostrum complex for pets according to claim 3, characterized in that: The pepsin is subjected to an embedding treatment: the pepsin is dissolved in an acetate buffer solution, then mixed with a chitosan-sodium alginate raw material in a ratio of 1:2, and then the embedded pepsin is prepared by a gelation method.

5. A method for preparing a hypoallergenic bovine colostrum complex for pets as claimed in any one of claims 1 to 4, comprising the steps of: (1) Add fresh bovine colostrum, hydrolyzed protein, trypsin, xyloproteinase, pepsin, glutamine and vitamin C into a reactor to react and obtain enzyme-killed bovine colostrum. in, The reaction pH is 5.3-5.8, the temperature is 48°C-55°C, the reaction time is 120-150min, and the ratio of trypsin, xyloproteinase and pepsin is 1:1:0.5; (2) premixing nano-hydroxyapatite and disodium ethylenediaminetetraacetate by ball milling, mixing the premixed mixture with bovine colostrum, and performing two-stage ultrasonic dispersion to obtain a nano-composite mixed solution; (3) Vitamin microcapsules were prepared by emulsification-solvent evaporation method, and ethyl cellulose and polyvinyl alcohol were used to encapsulate vitamin B1, vitamin B2, and vitamin B9 in the vitamin and trace element premix in microcapsules; (4) premixing the vitamin microcapsules prepared in the previous step with other vitamin and trace element premixes, and preparing supplementary granules with starch at a mass ratio of 1:5; (5) After uniformly mixing the nanocomposite mixed solution, fructooligosaccharide, β-glucan and fresh bovine colostrum obtained in step (2), the enzyme-killed bovine colostrum obtained in step (1) is added and stirred to obtain a first mixed bovine colostrum. Among them, the stirring speed is 30 ~40r / min, and the stirring time is 30 ~40min; (6) mixing the supplement particles obtained in step (4), the first mixed bovine colostrum obtained in step (5), and arginine to obtain a second mixed bovine colostrum, Wherein, the mixing temperature is 42 ~ 45 ° C, the stirring speed is 30 ~ 40r / min, and the stirring time is 40 ~ 45min; (7) Add Lactobacillus acidophilus to the second mixed bovine colostrum and stir. Wherein, the stirring temperature is 35-40°C, the stirring speed is 20-30r / min, and the stirring time is 20-30min; Then, the egg yolk immunoglobulin IgY is added to the above mixture and stirred to obtain the third mixed bovine colostrum. Wherein, the stirring temperature is 30~35°C, the stirring speed is 15~25r / min, and the stirring time is 15~20min; (8) Dissolving rosemary extract and sodium benzoate in sterile water to prepare a solution, and sequentially adding the third mixed bovine colostrum prepared in step (7), stirring and mixing, to prepare a bovine colostrum complex; (9) subjecting the bovine colostrum complex obtained in step (8) to plasma treatment and pasteurization in sequence to obtain a final product. Among them, the power of plasma treatment is 100~300W, the treatment time is 5~10min, the temperature of pasteurization is 72~75°C, and the time is 15~20s.

6. The method of producing a hypoallergenic bovine colostrum complex for pets according to claim 5, characterized in that: The hydrolyzed protein is prepared by the following steps: Fresh bovine colostrum is filtered, and then mixed with trypsin and xyloproteinase in a container for reaction. The reaction pH is 6 to 8, the temperature is 45°C to 55°C, and the reaction time is 2 to 4 hours. After the reaction is completed, the mixture is heated to 70°C to 80°C for 10 to 15 minutes, and then filtered and centrifuged to obtain hydrolyzed protein; Among them, according to the protein content in bovine colostrum, the mass ratio of papain to bovine colostrum protein is 1:100; the mass ratio of trypsin to bovine colostrum protein is 1:

100.

7. The method of producing a hypoallergenic bovine colostrum complex for pets according to claim 5, characterized in that: The speed and premixing time of the ball mill premixing in step (2) are set according to the following formula: when hour, , , when hour, , , when hour, , , Among them, m is the material amount in the ball mill, n is the rotation speed of the ball mill, and t is the premixing time.

8. The method of producing a hypoallergenic bovine colostrum complex for pets according to claim 5, characterized in that: The two-stage ultrasonic dispersion in step (2) includes a first-stage ultrasonic dispersion and a second-stage ultrasonic dispersion, wherein the parameters of the first-stage ultrasonic dispersion are P1=100W, f1=20000Hz, t1=5min, The parameters of the second stage ultrasonic dispersion are set by the following formula: when hour, , , , when hour, , , , when hour, , , , The power of the first stage ultrasonic dispersion is P1, the frequency is f1, and the processing time is t1. The power of the second stage ultrasonic dispersion is P2, the frequency is f2, and the processing time is t2. The uniformity of the mixture after the first stage of ultrasonic dispersion is U1, and the value range of U1 is 0 ~1.

9. The method of preparing a hypoallergenic bovine colostrum complex for pets according to claim 5, characterized in that: The ratio of the sodium benzoate in step (7) is 5:2; The temperature of the sterile water in which the rosemary extract is dissolved is 30-40°C, and the temperature of the sterile water in which the sodium benzoate is dissolved is 20-25°C.

10. The method of producing a hypoallergenic bovine colostrum complex for pets according to claim 9, characterized in that: In step (7), the dissolved rosemary extract solution is first added to the second mixed bovine colostrum, with a stirring speed of 100 to 200 r / min and a stirring time of 5 to 10 min; Then add the dissolved sodium benzoate solution, stirring at a speed of 300 ~500r / min and for 10 ~15min.

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