Method for inoculating cistanche with atriplex canescens

Abstract

The invention discloses a method for inoculating cistanche with atriplex canescens in the technical field of plant cultivation. The method comprises the following steps of host plant selection and cultivation, cistanche seed treatment, inoculation, cultivation management, harvesting and processing. The method has the advantages that the parasitic success rate is increased and can reach 80% or above generally; the atriplex canescens grows well, sufficient nutrients are provided for the cistanche deserticola, and the yield of the cistanche deserticola is remarkably increased and is increased by 30%-50% compared with a traditional method. And 3, the ecological benefit is remarkable: the planting of the atriplex canescens is beneficial to improving the soil structure and the ecological environment, and meanwhile, the growth of the cistanche deserticola also provides protection for the atriplex canescens, so that the mutualism of the atriplex canescens and the cistanche deserticola is realized, the vegetation coverage rate of a desertification region is enhanced, and the soil structure and the ecological environment are improved. And 4, the economic benefits are considerable, large-scale planting can be realized, a new way is provided for increasing income of farmers, and the method has good market prospects and economic benefits.

Description

Technical Field

[0001] The invention relates to the technical field of plant planting, in particular to a method for inoculating Atriplex canescens with Cistanche deserticola. Background Art

[0002] Cistanche deserticola is a precious medicinal plant with benefits such as tonifying the kidneys and strengthening yang, moistening the intestines and promoting bowel movements, earning it the nickname "desert ginseng." However, Cistanche deserticola is a parasitic plant and must rely on a host plant for growth. Traditional methods for cultivating Cistanche deserticola often use Haloxylon ammodendron as a host, but Haloxylon ammodendron grows slowly and has high requirements for soil and climatic conditions, resulting in limited Cistanche deserticola production. Furthermore, the need for ecological restoration in desertified areas is becoming increasingly urgent, and a cultivation method that can both increase Cistanche deserticola production and improve the ecological environment is urgently needed. Atriplex canescens is a drought- and salt-tolerant shrub with strong adaptability and ecological restoration capabilities, and is commonly used for vegetation restoration in desertified areas. However, traditional methods for cultivating Cistanche deserticola primarily rely on its natural parasites on plants such as Atriplex canescens. However, due to the destruction of the natural environment and excessive harvesting by humans, Cistanche deserticola resources are dwindling, making it difficult to meet market demand. Therefore, there is a lack of a method for inoculating Cistanche deserticola with Atriplex canescens to address these issues. Summary of the Invention

[0003] The invention aims to solve the shortcomings of the prior art and proposes a method for inoculating Cistanche deserticola with Atriplex quadrangularis.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] A method for inoculating Cistanche deserticola with Atriplex quadrangularis, the method comprising the following steps:

[0006] S1: Host plant selection and cultivation:

[0007] Select strong and well-developed Atriplex canescens as host plants, plant them in desertified areas, and use drip irrigation technology to provide adequate water to ensure that the Atriplex canescens is in good growth condition before inoculation.

[0008] S2: Cistanche deserticola seed treatment:

[0009] Soaking Cistanche deserticola seeds in a solution containing plant growth regulators for 12-24 hours to break seed dormancy and increase germination rate;

[0010] S3: Inoculation:

[0011] Inoculation is carried out during the vigorous growth period of Atriplex canescens. A small pit with a depth of 5-10 cm is dug at the root of Atriplex canescens. The inoculum formed by mixing Cistanche deserticola seeds with an appropriate amount of humus is evenly sprinkled into the pit. The soil is covered and lightly compacted. A layer of fine sand is covered on the surface of the seeds with a thickness of about 5-10 cm to maintain humidity and promote contact between the seeds and the host root system. A layer of plastic film is covered on the inoculation site to maintain soil moisture and temperature.

[0012] S4: Cultivation Management:

[0013] After inoculation, keep the soil moist and water regularly using a drip irrigation system to maintain soil moisture at 60%-70%. Add water and organic fertilizers in a timely manner. Use shade nets and plant companion plants to provide appropriate shade for Cistanche deserticola, and remove weeds regularly.

[0014] S5: Harvesting and processing:

[0015] When the Cistanche deserticola plant grows to 30-50 cm, it is harvested, cleaned, sliced ​​and dried in time to make medicinal materials.

[0016] Preferably, the plant growth regulator in step S2 is gibberellin.

[0017] Preferably, the amount of humus added in step S3 is 2-3 times the weight of Cistanche deserticola seeds.

[0018] Preferably, the drip irrigation technology in step S4 is irrigated once every 2-3 days, and the amount of water irrigated each time is 5-10 liters per Atriplex canescens plant.

[0019] Preferably, the companion plant in step S4 is Astragalus membranaceus.

[0020] Preferably, the shading rate of the sunshade net in step S4 is 30%-50%.

[0021] Beneficial effects:

[0022] The method of inoculating Cistanche deserticola with Atriplex canescens provided by the present invention has the following advantages: 1. Improving the parasitic success rate: Through scientific inoculation methods and meticulous management, the parasitic success rate of Cistanche deserticola is significantly improved, generally reaching more than 80%. 2. Increasing yield: Atriplex canescens grows well, providing sufficient nutrients for Cistanche deserticola, so that the yield of Cistanche deserticola is significantly improved, increasing by 30%-50% compared with traditional methods. 3. Significant ecological benefits: The cultivation of Atriplex canescens helps to improve soil structure and ecological environment, while the growth of Cistanche deserticola also provides protection for it, achieving a mutually beneficial symbiosis between the two, enhancing the vegetation coverage rate in desertified areas, and improving the soil structure and ecological environment. 4. Considerable economic benefits: Cistanche deserticola has high economic value. Through the method of the present invention, large-scale planting can be achieved, providing a new way to increase farmers' income, and having good market prospects and economic benefits. DETAILED DESCRIPTION

[0023] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Example 1

[0025] This embodiment provides a technical solution: a method for inoculating Cistanche deserticola with Atriplex quadrangularis, the method comprising the following steps:

[0026] S1: Host plant selection and cultivation:

[0027] Select strong and well-developed Atriplex canescens as host plants, plant them in desertified areas, and use drip irrigation technology to provide adequate water to ensure that the Atriplex canescens is in good growth condition before inoculation.

[0028] S2: Cistanche deserticola seed treatment:

[0029] Soaking Cistanche deserticola seeds in a solution containing plant growth regulators for 12-24 hours to break seed dormancy and increase germination rate;

[0030] S3: Inoculation:

[0031] Inoculation is carried out during the vigorous growth period of Atriplex canescens. A small pit with a depth of 5-10 cm is dug at the root of Atriplex canescens. The inoculum formed by mixing Cistanche deserticola seeds with an appropriate amount of humus is evenly sprinkled into the pit. The soil is covered and lightly compacted. A layer of fine sand is covered on the surface of the seeds with a thickness of about 5-10 cm to maintain humidity and promote contact between the seeds and the host root system. A layer of plastic film is covered on the inoculation site to maintain soil moisture and temperature.

[0032] S4: Cultivation Management:

[0033] After inoculation, keep the soil moist and water regularly using a drip irrigation system to maintain soil moisture at 60%-70%. Add water and organic fertilizers in a timely manner. Use shade nets and plant companion plants to provide appropriate shade for Cistanche deserticola, and remove weeds regularly.

[0034] S5: Harvesting and processing:

[0035] When the Cistanche deserticola plant grows to 30-50 cm, it is harvested, cleaned, sliced ​​and dried in time to make medicinal materials.

[0036] In practice, Atriplex canescens was used for the first time as a host plant for Cistanche deserticola, leveraging its drought and saline-alkali tolerance to expand its cultivation range, making it particularly suitable for desertification. Seed treatment and trench inoculation significantly improved the parasitic efficiency and germination rate of Cistanche deserticola. Seed treatment can break the dormancy of Cistanche deserticola seeds and increase germination rate; trench inoculation allows Cistanche deserticola seeds to better contact the host root system, increasing parasitic success. The combination of Atriplex canescens and Cistanche deserticola not only increases Cistanche deserticola yields but also enhances vegetation cover in desertification areas, improving soil structure and the ecological environment.

[0037] Example 2

[0038] Based on Example 1, the plant growth regulator in step S2 is gibberellin.

[0039] During the specific implementation, select healthy and plump Atriplex canescens seeds, soak them in 0.1% potassium permanganate solution for 30 minutes for disinfection, and then rinse them with clean water. Spread the treated seeds evenly in the seedling tray, cover with a thin layer of sand, and keep the soil moist. Germination is carried out at a temperature of 28°C, and seedlings will emerge after about 8 days. When the Atriplex canescens seedlings grow to 8 cm, transplant them into the field with a plant spacing of 35 cm and a row spacing of 55 cm, ensuring that the soil is loose and well-drained. The plant spacing for transplanting Atriplex canescens is 35 cm and the row spacing is 55 cm.

[0040] Cistanche deserticola seeds are soaked in a solution containing a plant growth regulator (gibberellic acid) for 12-24 hours to break seed dormancy and increase germination. This treatment significantly increases the germination rate of Cistanche deserticola seeds, laying a good foundation for subsequent parasitic growth.

[0041] Example 3

[0042] Based on the above embodiment, the amount of humus soil added in step S3 is 2-3 times the weight of Cistanche deserticola seeds.

[0043] In practice, inoculation should be carried out during the peak growth period of Atriplex canescens, generally in spring or autumn. During this period, the root system of Atriplex canescens is active, which is conducive to the parasitism and growth of Cistanche deserticola seeds. Inoculation site: Select a root segment with a diameter of 0.5-1.0 cm at the root of the Atriplex canescens for inoculation. Selecting the appropriate root segment can increase the success rate of Cistanche deserticola parasitism. Inoculation technique: Mix Cistanche deserticola seeds with an appropriate amount of humus soil to form an inoculum. Humus soil provides the nutrients necessary for Cistanche deserticola to promote germination and growth. Dig a small pit 5-10 cm deep at the root of the Atriplex canescens. Evenly sprinkle the inoculum into the pit, then cover with soil and gently compact. Cover the seeds with a layer of fine sand approximately 5-10 cm thick to maintain moisture and promote contact between the seeds and the host root system. Cover the inoculation site with a layer of plastic film to maintain soil moisture and temperature. Plastic film can effectively maintain soil moisture, prevent water evaporation, and provide a good environment for the germination and growth of Cistanche deserticola.

[0044] Example 4

[0045] Based on the above embodiment, the irrigation frequency of the drip irrigation technology in step S4 is once every 2-3 days, and the irrigation amount each time is 5-10 liters of water per Atriplex canescens plant.

[0046] Regarding water management: Keep the soil moist after inoculation and avoid overwatering, which can affect the growth of Atriplex and Cistanche. Use a drip irrigation system for regular watering, maintaining soil moisture between 60% and 70%. Fertilization: Apply appropriate amounts of organic and compound fertilizers during the growth period to promote healthy growth. These fertilizers provide the nutrients necessary for Atriplex and Cistanche, promoting healthy growth. Pest and disease control: Regularly inspect the plants to detect and prevent pests and diseases, ensuring the healthy growth of Atriplex and Cistanche. Pest and disease control can effectively reduce the impact of pests and diseases on the plants and ensure their healthy growth.

[0047] Example 5

[0048] Based on the above embodiment, the companion plant in step S4 is Astragalus membranaceus, and the shading rate of the sunshade net is 30%-50%.

[0049] In specific implementation, shade management: Use shade nets or plant companion plants (Astragalus membranaceus) to provide moderate shade for Cistanche deserticola to avoid direct sunlight. Moderate shade can provide suitable light conditions for the growth of Cistanche deserticola and promote its healthy growth.

[0050] Example 6

[0051] Based on the above embodiment, step S5, harvesting and processing, is specifically implemented as follows: Harvest the Cistanche deserticola plants when they reach 30-50 cm in length. Careful handling is required during harvesting to avoid damaging the roots of the Atriplex quadrangularis. Processing: After harvesting, the plants are promptly cleaned, sliced, and dried to produce the medicinal material. These cleaning, slicing, and drying steps effectively remove impurities and moisture from the Cistanche deserticola, enhancing its medicinal value and shelf life.

[0052] The present invention provides a method for inoculating Cistanche deserticola with Atriplex serrata. Atriplex serrata is utilized as the host plant of Cistanche deserticola. By optimizing host plant selection, inoculation technology and cultivation management, efficient parasitism and growth of Cistanche deserticola are achieved. Specifically, first, Atriplex serrata with robust growth and well-developed root system is selected as the host plant, and planted in desertified areas. Drip irrigation technology is adopted to provide appropriate amount of water to ensure that Atriplex serrata has a good growth state before inoculation. Then, Cistanche deserticola seeds are soaked in a solution containing plant growth regulators and processed to break seed dormancy and improve germination rate. Then, inoculate in the vigorous growth phase of Atriplex serrata, select suitable root segment to inoculate, and adopt trench inoculation technology so that Cistanche deserticola seeds are better contacted with host root system, improve parasitism success rate. After inoculation, water is regularly applied through a drip irrigation system to maintain soil moisture, and organic fertilizer and compound fertilizer are applied in appropriate amounts to promote the healthy growth of Atriplex canescens and Cistanche deserticola. A sunshade net or companion plants are used to provide appropriate shade for Cistanche deserticola to avoid direct sunlight. When the Cistanche deserticola plant grows to a certain height, it is harvested and promptly cleaned, sliced, dried, and made into a medicinal material. Through this series of optimization measures, the efficient parasitism and growth of Cistanche deserticola are achieved, and ecological restoration in desertified areas is promoted simultaneously. Through the implementation of the present invention, the parasitism success rate of Cistanche deserticola can be increased to more than 80%, and the output increases by 30%-50% compared with traditional methods. Simultaneously, the planting of Atriplex canescens effectively improves the soil quality and ecological environment in desertified areas, achieving a win-win situation of economic benefit and ecological benefit.

[0053] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for inoculating Cistanche deserticola with Atriplex quadrangularis, characterized in that: The method comprises the following steps: S1: Host plant selection and cultivation: Select strong and well-developed Atriplex canescens as host plants, plant them in desertified areas, and use drip irrigation technology to provide appropriate amount of water to ensure that Atriplex canescens is in good growth state before inoculation; S2: Cistanche deserticola seed treatment: Soak the Cistanche deserticola seeds in a solution containing a plant growth regulator for 12-24 hours to break seed dormancy and increase germination rate; S3: Inoculation: Inoculation is carried out during the vigorous growth period of Atriplex four-winged. A small pit with a depth of 5-10 cm is dug at the root of Atriplex four-winged. The inoculant formed by mixing Cistanche deserticola seeds with an appropriate amount of humus soil is evenly sprinkled into the pit, and the soil is covered and lightly compacted. A layer of fine sand is covered on the surface of the seeds with a thickness of about 5-10 cm to maintain humidity and promote contact between the seeds and the host root system. A layer of plastic film is covered on the inoculation site to maintain soil humidity and temperature. S4: Cultivation management: Keep the soil moist after inoculation, use a drip irrigation system to water regularly, keep the soil moisture at 60%-70%, add water and organic fertilizer in time, use shade nets and plant companion plants to provide appropriate shade for Cistanche deserticola, and remove weeds regularly; S5: Harvesting and processing: When the Cistanche deserticola plant grows to 30-50 cm, it is harvested, cleaned, sliced ​​and dried in time to make medicinal materials.

2. The method for inoculating Cistanche deserticola with Atriplex quadrangularis according to claim 1, characterized in that: The plant growth regulator in step S2 is gibberellin.

3. The method for inoculating Cistanche deserticola with Atriplex quadrangularis according to claim 1, characterized in that: The amount of humus soil added in step S3 is 2-3 times the weight of Cistanche deserticola seeds.

4. The method for inoculating Cistanche deserticola with Atriplex quadrangularis according to claim 1, characterized in that: The irrigation frequency of the drip irrigation technology in step S4 is once every 2-3 days, and the amount of each irrigation is 5-10 liters of water per plant of Atriplex canescens.

5. The method for inoculating Cistanche deserticola with Atriplex quadrangularis according to claim 1, characterized in that: The companion plant in step S4 is Astragalus membranaceus.

6. The method for inoculating Cistanche deserticola with Atriplex quadrangularis according to claim 1, characterized in that: The shading rate of the sunshade net in step S4 is 30%-50%.