External traditional Chinese medicine preparation for preventing and treating HPV infection

Abstract

The invention relates to an external traditional Chinese medicine preparation for preventing and treating HPV (human papillomavirus) infection, which belongs to the technical field of external medicines and comprises the following raw materials: 1-10 parts of gallnut, 1-10 parts of szechwan chinaberry fruit, 1-10 parts of golden cypress, 1-10 parts of fructus cnidii, 1-10 parts of radix sophorae flavescentis, 1-10 parts of cyrtomium rhizome, 1-10 parts of rhizoma atractylodis, 1-10 parts of cortex dictamni, 0-10 parts of calomel, 0-10 parts of cinnabar and 0-10 parts of borneol. The external traditional Chinese medicine preparation disclosed by the invention is locally administrated through the vagina, so that the active ingredients directly reach the focus, and the external traditional Chinese medicine preparation is safe and convenient, and is suitable for resisting HPV infection, cervicitis, cervical erosion, pelvic inflammation, bacterial vaginitis, trichomonas vaginitis, mixed vaginitis, peculiar smell of private parts, pruritus of private parts, dryness of private parts, abnormal leucorrhea and pruritus and causalgia of vulva.

Description

Technical Field

[0001] The invention belongs to the technical field of external medicines, and specifically relates to an external Chinese medicine preparation for preventing and treating HPV infection. Background Art

[0002] Human papillomavirus (HPV) is a non-enveloped icosahedral capsid protein virus, a spherical DNA virus, belonging to the genus Papillomavirus. Based on the relationship between HPV infection and the risk of cervical cancer, it is divided into low-risk and high-risk types. Low-risk HPVs are mainly HPV6, HPV11, HPV30, HPV42 and HPV43, which mainly cause benign lesions such as low-grade cervical intraepithelial neoplasia and genital warts. High-risk HPVs are mainly HPV16, HPV18, HPV31, HPV33 and HPV52, which mainly cause lesions such as cervical intraepithelial neoplasia, cervical cancer and external genital cancer.

[0003] At present, clinical treatments for HPV are divided into Western medicine, Chinese medicine and a combination of Chinese and Western medicine. Western medicine treatment generally involves physical therapy such as laser, freezing, microwave and ozone fumigation, surgical treatment and drug treatment such as interferon and vaccine. Among them, physical therapy and surgical treatment are prone to cause organic damage, while drug treatment focuses on vaccine research, mainly preventive vaccination, while therapeutic vaccines have not yet been realized.

[0004] Traditional Chinese medicine uses oral administration, topical application or a combination of the two for treatment. Topical preparations such as the Wart Poisoning developed by Guangdong Provincial Hospital of Traditional Chinese Medicine can treat multiple diseases caused by HPV: cervical precancerous lesions, recurrent condyloma acuminatum, flat warts, etc. In addition, there is a compound preparation called Pateling, which is composed of more than 10 Chinese medicines such as honeysuckle, isatis indigo leaf, sophora flavescens, and cnidium monnieri. It has been proven that it can inhibit the expression of HPV-related genes (such as E6 and E7) and affect the activity of the Wnt / β-catenin signaling pathway, thereby inhibiting the proliferation of HPV and the malignant transformation of cells.

[0005] With the continuous development of traditional Chinese medicine, more and more patients and medical practitioners have placed greater expectations on Chinese medicine external preparations. Therefore, it is of great significance to develop an external Chinese medicine preparation for the prevention and treatment of HPV infection. Summary of the invention

[0006] The purpose of the present invention is to provide an external Chinese medicine preparation for preventing and treating HPV infection.

[0007] The technical solution adopted by the present invention to achieve its purpose is as follows:

[0008] An external Chinese medicine preparation for preventing and treating HPV infection. The raw materials include, by weight, 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea, 1-10 parts of Dictamni cortex, 0-10 parts of Calomel, 0-10 parts of Cinnabar and 0-10 parts of Borneol.

[0009] Preferably, the raw materials are weighed in proportion, ground into powder, and added with auxiliary materials for granulation or tableting.

[0010] Preferably, the auxiliary material comprises a wetting agent and / or a binder.

[0011] Preferably, 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea, and 1-10 parts of Dictamni cortex are weighed by mass, and extracted by percolation, decoction, or enzymolysis to obtain an extract;

[0012] According to the mass proportions, 0-10 parts of calomel, 0-10 parts of cinnabar and 0-10 parts of borneol are added to the extract to obtain a semi-finished product, which is then added with auxiliary materials to prepare an ointment, a suppository, a gel, an emulsion, a lotion, a spray or a tablet.

[0013] Preferably, the extract is prepared by weighing 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes macrocephala, and 1-10 parts of Dictamni cortex by mass, adding 8-10 times the total mass of the above drugs in water, soaking for 30-40 minutes, decocting twice, each time for 1-3 hours, combining the filtrate, filtering, and concentrating to obtain the extract.

[0014] Preferably, the extract is prepared by weighing 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea, and 1-10 parts of Dictamni cortex by mass, crushing, passing through a 100-mesh sieve, adding 60%-75% ethanol to soak for at least 2 hours, the amount of alcohol used being 10-20 times the total mass of the above raw materials, and after soaking, percolating the soaking liquid through a percolation tank at a percolation liquid flow rate of 15-20 mL / min, collecting the percolation liquid, recovering the ethanol under reduced pressure, and concentrating to obtain the extract.

[0015] Preferably, the gelling agent comprises, by weight percentage, 20-30% of the semi-finished product, 2-3% of chitosan, 1-2% of carbomer, 5-10% of glycerol and the balance of purified water.

[0016] Preferably, a formulated amount of water is added to a liquid preparation tank, carbomer and chitosan are added, stirred to swell, and then the semi-finished product is added, and stirring and mixing are continued, and finally glycerol is added and stirred evenly to obtain a gel preparation.

[0017] The beneficial effects of the present invention are:

[0018] The external Chinese medicine preparation of the present invention forms a powerful prevention and treatment system of antibacterial and anti-inflammatory, heat-clearing and damp-drying, insecticidal and antipruritic through the synergistic effect of multiple Chinese medicines. Among them, the antibacterial and anti-inflammatory effects of Chinese medicines such as Galla chinensis, Cortex phellodendri, and Radix Sophorae flavescentis can directly inhibit the replication and spread of HPV virus; the insecticidal effects of Chinese medicines such as Fructus cnidii and Fructus toosendan can improve the internal environment and reduce the virus breeding conditions; the damp-drying effects of Chinese medicines such as Atractylodes lancea and Dictamni can improve the hot and humid environment in the body, thereby indirectly resisting the virus; Chinese medicines such as Calomel, Cinnabar, and Borneol play an auxiliary therapeutic role.

[0019] The external Chinese medicine preparation of the present invention is locally administered through the vagina so that the effective ingredients can directly reach the lesion, which is safe and convenient. It is suitable for preventing HPV infection, cervicitis, cervical erosion, pelvic inflammatory disease, bacterial vaginitis, Trichomonas vaginitis, mixed vaginitis, private part odor, private part itching, private part dryness, abnormal leucorrhea, and vulvar itching and burning pain. DETAILED DESCRIPTION

[0020] The present invention is described in detail below in conjunction with specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work should belong to the scope of protection of the present invention. Anything not fully described in the present invention is prior art.

[0021] The pharmacological effects of the present invention are as follows:

[0022] Gallnut has the effects of astringent and antidiarrhea, detoxification and swelling, astringent and antidiarrhea, and astringent sweat and semen. It can clear away heat and detoxify, reduce swelling and relieve pain, inhibit bacterial growth, reduce inflammatory reactions, and promote wound healing. When combined with Phellodendron chinense and Sophora flavescens, the antibacterial and anti-inflammatory effects of the overall composition can be enhanced, effectively inhibiting local inflammatory reactions caused by HPV infection and reducing the spread of infection.

[0023] Toosendan fruit has the effects of soothing liver and relieving heat, promoting qi and relieving pain, and killing insects; it can expel intestinal parasites. When combined with Cnidium monnieri, it can enhance the indirect inhibitory effect on HPV virus by improving the internal environment and reducing the conditions for virus breeding.

[0024] Phellodendron chinense has the effects of clearing away heat and dampness, antibacterial and anti-inflammatory, astringent and antidiarrheal, antioxidant, and anti-allergic. It contains effective antibacterial ingredients such as berberine, which can inhibit and kill a variety of bacteria and fungi, and also has a certain inhibitory effect on HPV virus. Combining it with Sophora flavescens and Cnidium monnieri can enhance the antibacterial, anti-inflammatory, heat-clearing and dampness-drying effects of the overall composition.

[0025] Cnidium monnieri has the effects of drying dampness and dispelling wind, killing insects and relieving itching, and warming the kidney and strengthening yang. When it is combined with Sophora flavescens and Phellodendron amurense, the insecticidal and itchy, heat-clearing and dampness-drying effects of the overall composition can be enhanced. At the same time, its kidney-warming and yang-strengthening effects may also help improve the body's overall immunity, thereby indirectly resisting HPV virus.

[0026] Sophora flavescens has the effects of clearing away heat and dampness, killing insects and acting as a diuretic; it can fight fungal and parasitic infections and improve private itching. When combined with Phellodendron chinense and Cnidium monnieri, it can form a powerful antibacterial and anti-inflammatory, heat-clearing and dampness-clearing lineup to jointly resist HPV virus.

[0027] Guanzhong has the effects of clearing away heat, detoxifying, stopping bleeding and killing insects. When it is combined with Phellodendron chinense, the heat-clearing, detoxifying and insecticidal effects of the overall composition can be enhanced.

[0028] Atractylodes has the effects of drying dampness and strengthening the spleen, dispelling wind and dispersing cold. When it is combined with Sophora flavescens, Cnidium monnieri, Phellodendron chinense, and Dictamni peel, the drying effect of the overall composition can be enhanced, thereby improving the damp and hot environment in the body and reducing the breeding of viruses. At the same time, it can enhance local immune function and improve the body's ability to clear HPV.

[0029] Dictamni peel has the effects of clearing away heat and dampness, dispelling wind and detoxifying. When combined with Phellodendron chinense and Sophora flavescens, the heat-clearing and dampness-dispelling and detoxifying effects of the overall composition can be enhanced.

[0030] Light powder has the effects of killing insects and attacking toxins, and stopping sores and itching; cinnabar has the effects of clearing the heart and calming the nerves, calming the nerves and detoxifying; borneol has the effects of opening the mind and refreshing the mind, clearing away heat and relieving pain. These three herbs mainly play an auxiliary therapeutic role in the composition, enhancing the insecticidal, antipruritic, and heat-clearing and detoxifying effects of the overall composition. At the same time, the opening and refreshing effect of borneol may also help improve the local discomfort or pain symptoms caused by HPV infection.

[0031] Example 1

[0032] Preparation of extract: Take 10g of Galla chinensis, 10g of Toosendan fruit, 10g of Phellodendron chinense, 10g of Cnidium monnieri, 10g of Sophora flavescens, 10g of Cyperus rotundus, 10g of Atractylodes lancea, and 10g of Dictamni peel, add 10 times the total mass of the above drugs in water, soak for 30 minutes, decoct twice, each time for 2 hours, combine the filtrate, filter, and concentrate to obtain the extract.

[0033] Take 2 g of calomel, 2 g of cinnabar and 2 g of borneol and add them to the extract to obtain a semi-finished product.

[0034] Example 2

[0035] Preparation of extract: Take 5g of Galla chinensis, 8g of Toosendan fruit, 7g of Phellodendron chinense, 4g of Cnidium monnieri, 10g of Sophora flavescens, 6g of Cynomorium fortunei, 9g of Atractylodes macrocephala, and 2g of Dictamni cortex, add 8 times the total mass of the above drugs in water, soak for 30min, decoct twice, each time for 2h, combine the filtrates, filter, and concentrate to obtain the extract.

[0036] Take 1g of calomel, 1g of cinnabar and 3g of borneol and add them to the extract to obtain a semi-finished product.

[0037] Example 3

[0038] Preparation of extract: Take 9g of Galla chinensis, 3g of Toosendan fruit, 5g of Phellodendron chinense, 8g of Cnidium monnieri, 5g of Sophora flavescens, 10g of Cyperus rotundus, 4g of Atractylodes macrocephala, and 8g of Dictamni cortex, add 8 times the total mass of the above drugs in water, soak for 30min, decoct twice, each time for 2h, combine the filtrate, filter, and concentrate to obtain the extract.

[0039] Take 2 g of calomel, 1 g of cinnabar and 2 g of borneol and add them to the extract to obtain a semi-finished product.

[0040] Example 4

[0041] Preparation of extract: Weigh 10g of Galla chinensis, 10g of Toosendan fruit, 10g of Phellodendron chinense, 10g of Cnidium monnieri, 10g of Sophora flavescens, 10g of Cyperus rotundus, 10g of Atractylodes lancea, and 10g of Dictamni cortex, crush them, pass through a 100-mesh sieve, add 60% ethanol and soak for at least 2h, the amount of alcohol used being 20 times the total mass of the above raw materials, after soaking, percolate the soaking liquid through a percolation tank, the percolation liquid flow rate being 15mL / min, collect the percolation liquid, recover the ethanol under reduced pressure, and concentrate to obtain the extract.

[0042] Take 1g of calomel, 1g of cinnabar and 1g of borneol and add them to the extract to obtain a semi-finished product.

[0043] Example 5

[0044] Preparation of extract: Weigh 7g of Galla chinensis, 5g of Toosendan fruit, 10g of Phellodendron chinense, 10g of Cnidium monnieri, 10g of Sophora flavescens, 4g of Cyperus rotundus, 8g of Atractylodes macrocephala, and 6g of Dictamni cortex, crush them, pass through a 100-mesh sieve, add 60% ethanol and soak for at least 2h, the amount of alcohol used being 15 times the total mass of the above raw materials, after soaking, percolate the soaking liquid through a percolation tank, the percolation liquid flow rate being 18mL / min, collect the percolation liquid, recover the ethanol under reduced pressure, and concentrate to obtain the extract.

[0045] Take 3 g of calomel, 1 g of cinnabar and 2 g of borneol and add them to the extract to obtain a semi-finished product.

[0046] Example 6

[0047] Preparation of extract: Weigh 9g of Galla chinensis, 6g of Toosendan fruit, 10g of Phellodendron chinense, 10g of Cnidium monnieri, 10g of Sophora flavescens, 3g of Cyperus rotundus, 5g of Atractylodes macrocephala, and 10g of Dictamni cortex, crush them, pass through a 100-mesh sieve, add 75% ethanol and soak for at least 2h, the amount of alcohol used being 10 times the total mass of the above raw materials, after soaking, percolate the soaking liquid through a percolation tank, the percolation liquid flow rate being 20mL / min, collect the percolation liquid, recover the ethanol under reduced pressure, and concentrate to obtain the extract.

[0048] Take 1g of calomel, 1g of cinnabar and 2g of borneol and add them to the extract to obtain a semi-finished product.

[0049] Example 7

[0050] A traditional Chinese medicine preparation for external use for preventing and treating HPV infection comprises 10g of Galla chinensis, 10g of Toosendan fruit, 10g of Phellodendron chinense, 10g of Cnidium monnieri, 10g of Sophora flavescens, 10g of Cyperus rotundus, 10g of Atractylodes lancea, 10g of Dictamni cortex, 10g of Light Powder, 10g of Cinnabar, and 10g of Borneol.

[0051] Weigh the raw materials according to the proportion, grind them into powder and pass them through a 100-mesh sieve for later use;

[0052] Take 12 g of chitosan and 8 g of carbomer, add 300 g of water to swell, then add the sieved raw material powder, stir and mix, and finally add 15 g of glycerol and stir evenly to obtain a gel preparation.

[0053] Example 8

[0054] 20% of the semi-finished product prepared in Example 1, 2% of chitosan, 1% of carbomer, 5% of glycerol and the balance of purified water.

[0055] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0056] Example 9

[0057] 30% of the semi-finished product prepared in Example 1, 3% of chitosan, 2% of carbomer, 10% of glycerol and the balance of purified water.

[0058] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0059] Example 10

[0060] 25% of the semi-finished product prepared in Example 1, 2.5% of chitosan, 1.5% of carbomer, 8% of glycerol and the balance of purified water.

[0061] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0062] Embodiment 11

[0063] 22% of the semi-finished product prepared in Example 4, 2% of chitosan, 1% of carbomer, 6% of glycerol and the balance of purified water.

[0064] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0065] Example 12

[0066] 24% of the semi-finished product prepared in Example 4, 2.5% of chitosan, 1.5% of carbomer, 7% of glycerol and the balance of purified water.

[0067] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0068] Embodiment 13

[0069] The semi-finished product prepared in Example 4 is 28%, chitosan is 3%, carbomer is 2%, glycerol is 9% and the balance is purified water.

[0070] Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the extract, continue to stir and mix, and finally add glycerin and stir evenly to obtain a gel preparation.

[0071] Vaginal irritation test

[0072] Six healthy adult female domestic rabbits were used in the experiment, with three animals in each sample group and control group. The test group was injected with 1 mL of sample gel, and the control group was injected with 1 mL of 0.9% saline injection. The overflowed liquid was gently wiped off with soft tissue paper, and the above steps were repeated continuously at intervals of 24 hours for 5 consecutive days. The vaginal orifice and perineal discharge, erythema and irritation were recorded 24 hours after the first contact and before each experimental operation. 24 hours after the last contact, the animals were painlessly killed, the vagina was completely cut off and then cut longitudinally to check the irritation, damage and necrosis of the epithelial tissue layer. The vaginal tissue was fixed in 15% formalin fixative and then histologically evaluated.

[0073] The microscopic evaluation scores of the experimental group animals were added and then divided by the total number of observations to obtain the average score of the experimental group. The irritation index was obtained by subtracting the average score of the control group from the average score of the experimental group. The irritation index of 0 was no irritation, 1 to 4 was very slight irritation, 5 to 8 was mild irritation, 9 to 11 was moderate irritation, and 12 to 16 was severe irritation.

[0074] The test results are shown in Table 1.

[0075] Table 1

[0076] deal with Average score of the experimental group The average score of the control group Stimulation Index Example 7 2 2 0 Example 8 2 2 0 Example 9 2 2 0 Example 10 2 2 0 Embodiment 11 2 2 0 Example 12 2 2 0 Example 13 2 2 0

[0077] From the experimental results in Table 1, it can be seen that the external gel Chinese medicine preparation of the present invention has no irritation to vaginal tissue.

[0078] Clinical trials

[0079] Usage: Equipped with a delivery device for external vaginal administration. The delivery device is made of medical polyethylene. The chitosan vaginal gel is colorless to light yellow gel, free of impurities, 3g / tube, 7 days a course of treatment; remove the cap of the gel delivery device, the outer surface of the catheter should be smooth, without sharp edges and burrs. The viscosity is not less than 1000nPas; the pH is 3.8-5.8; the total content of heavy metals is not more than 20μg / g.

[0080] Case 1

[0081] 42 years old, gynecological itching, sticky secretions, odor, cervical exfoliated cells detected HPV virus, HPV6 positive, early infection, one bottle per day (Example 9), used for 2 months. Re-examination, no itching, secretions significantly reduced, no odor, HPV6 negative.

[0082] Case 2

[0083] 38 years old, cervical erosion, itching, sticky secretions, odor, cervical exfoliated cells detected HPV virus, the result was HPV52 positive, mid-term infection, two sticks a day (Example 9), continuous use for 3 months. Reexamination, erosion symptoms are significantly alleviated, no itching symptoms, secretions are significantly reduced, no odor, HPV52 is negative.

[0084] Case 3

[0085] 46 years old, cervical erosion, itching and private dryness, more sticky secretions, peculiar smell, cervical exfoliated cells detected HPV virus, the results were HPV16, HPV18 positive, early infection, one stick a day (Example 9), continuous use for 2 months. Reexamination, no erosion, itching symptoms, private dryness symptoms significantly alleviated, secretions significantly reduced, no peculiar smell, HPV16, HPV18 negative.

[0086] Case 4

[0087] 44 years old, cervical erosion, occasional itching, sticky secretions, odor, lower abdominal distension and pain, cervical exfoliated cells tested for HPV virus, the result was HPV44 positive, early infection, one tablet a day (Example 9), used continuously for 2 months. Reexamination, erosion symptoms were significantly alleviated, no itching symptoms, secretions were significantly reduced, no odor, occasional lower abdominal distension and pain, HPV44 was negative.

[0088] Case 5

[0089] 51 years old, gynecological itching and pain, white sticky secretions, slight odor, lower abdominal pain, cervical exfoliated cells tested for HPV virus, the result was HPV11 positive, early infection, one tablet a day (Example 9), used for 2 months. Reexamination, no itching and pain symptoms, secretions significantly reduced, no odor, lower abdominal pain significantly improved, HPV11 negative.

[0090] Case 6

[0091] 36 years old, leucorrhea color is yellow, gynecological pruritus, no peculiar smell, more secretions, no abdominal pain, regular menstruation, examination of leucorrhea is bacterial vaginitis, one (Example 9) a day, continuous use for 14 days. Recheck, leucorrhea is normal, normal amount is restored.

[0092] Case 7

[0093] 50 years old, mixed vaginitis, persistent pruritus of vulva, increased leucorrhea, no abdominal pain, one tablet a day (Example 9), continuous use for 14 days. Recheck, leucorrhea is normal, normal amount is restored, no pruritus symptoms.

[0094] The specific implementation methods of the present invention have been described in detail, but are not limited to the embodiments listed in the present invention. Any obvious changes made by those skilled in the art based on the teachings of the present invention are within the scope of protection of the present invention.

Claims

1. A Chinese medicine preparation for external use for preventing and treating HPV infection, characterized in that: By weight, the raw materials include 1-10 parts of gallnut, 1-10 parts of toosendan seeds, 1-10 parts of phellodendron, 1-10 parts of cnidium monnieri, 1-10 parts of sophora flavescens, 1-10 parts of cyrtomium, 1-10 parts of atractylodes, 1-10 parts of white moss peel, 0-10 parts of talcum powder, 0-10 parts of cinnabar, and 0-10 parts of borneol.

2. The external Chinese medicine preparation for preventing and treating HPV infection according to claim 1, characterized in that: The raw materials are weighed in proportion, ground into powder, and added with auxiliary materials to form granules, tablets, or gel for use.

3. The external Chinese medicine preparation for preventing and treating HPV infection according to claim 1, characterized in that: Excipients include wetting agents and / or binders.

4. The external Chinese medicine preparation for preventing and treating HPV infection according to claim 1, characterized in that: Weigh 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea, and 1-10 parts of Dictamni cortex by mass, and extract the Chinese medicine by percolation, decoction, or enzymolysis to obtain an extract; According to the mass proportions, 0-10 parts of calomel, 0-10 parts of cinnabar and 0-10 parts of borneol are added to the extract to obtain a semi-finished product, which is then added with auxiliary materials to prepare an ointment, a suppository, a gel, an emulsion, a lotion, a spray or a tablet.

5. A Chinese medicinal preparation for external use for preventing and treating HPV infection according to claim 1 or 4, characterized in that: Preparation of extract: weigh 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea, and 1-10 parts of Dictamni cortex by mass, add 8-10 times the total mass of the above drugs in water, soak for 30-40 minutes, decoct twice, each time for 1-3 hours, combine the filtrates, filter, and concentrate to obtain an extract.

6. A Chinese medicinal preparation for external use for preventing and treating HPV infection according to claim 1 or 4, characterized in that: Preparation of extract: weigh 1-10 parts of Galla chinensis, 1-10 parts of Toosendan fruit, 1-10 parts of Phellodendron chinense, 1-10 parts of Cnidium monnieri, 1-10 parts of Sophora flavescens, 1-10 parts of Cyperus rotundus, 1-10 parts of Atractylodes lancea and 1-10 parts of Dictamni cortex by mass, crush, pass through a 100-mesh sieve, add 60%-75% ethanol and soak for at least 2 hours, the amount of alcohol used is 10-20 times the total mass of the above raw materials, after soaking, percolate the soaking liquid through a percolation tank, the percolation liquid flow rate is 15-20 mL / min, collect the percolation liquid, recover the ethanol under reduced pressure, and concentrate to obtain the extract.

7. The external Chinese medicine preparation for preventing and treating HPV infection according to claim 4, characterized in that: The gelling agent comprises, by weight percentage, 20-30% of semi-finished products, 2-3% of chitosan, 1-2% of carbomer, 5-10% of glycerol and the balance of purified water.

8. The external Chinese medicine preparation for preventing and treating HPV infection according to claim 7, characterized in that: Add the formulated amount of water into the liquid preparation tank, add carbomer and chitosan, stir to swell, then add the semi-finished product, continue to stir and mix, and finally add glycerol, stir evenly to obtain a gel preparation.