Formula for relieving sore throat and reducing phlegm and preparation method thereof

Abstract

The invention provides a formula for relieving sore-throat and reducing phlegm and a preparation method thereof, and relates to the field of formulas for relieving sore-throat and reducing phlegm. The formula comprises 12-14 parts of poria cocos, 30-50 parts of radix asteris, 10-15 parts of semen lepidii, 8-12 parts of fructus amomi rotundus, 8-12 parts of folium ginkgo, 10-25 parts of a pinellia ternate agent, 10-15 parts of herba houttuyniae, 12-14 parts of fructus forsythiae, 10-16 parts of fructus arctii, 3-7 parts of water chestnut, 8-10 parts of sea blubber skin, 10-15 parts of pelvetia silquosa, 10-15 parts of semen sinapis, 8-10 parts of loquat, 28-36 parts of platycodon grandiflorum leaves, 18-25 parts of rhizoma belamcandae, 16-24 parts of liquorice and 8-15 parts of eucalyptus leaves. According to the formula capable of relieving sore throat and reducing phlegm, compatibility is carried out according to the principle of monarch, minister, assistant and guide, the unique medicine property and effect of the formula are achieved, the synergistic effect is achieved through mixing, the medicine effect is enhanced and balanced, and the medicine effect is improved; the effect of improving the immunity is achieved.

Description

Technical Field

[0001] The invention relates to the field of throat-clearing and phlegm-resolving formulas, and in particular to a throat-clearing and phlegm-resolving formula and a preparation method thereof. Background Art

[0002] Throat relief usually refers to relieving or eliminating throat discomfort, such as sore throat, dry throat, pain, hoarseness and other symptoms. This is usually caused by inflammation, infection or other irritation in the throat. Throat relief can be achieved in many ways, such as using drugs with the effect of clearing heat and relieving throat, or through some physical methods such as atomization inhalation, to relieve throat symptoms.

[0003] Expectorant means promoting the discharge of respiratory secretions through drugs or other methods to relieve symptoms such as cough and shortness of breath. Under normal circumstances, the respiratory tract secretes a small amount of mucus to moisten and protect the lungs, but when there is infection, inflammation or other reasons, mucus secretion may increase and form sputum. If sputum cannot be discharged in time, it may block the airway and cause discomfort. Expectorant drugs can dilute sputum to make it easier to cough up, or increase the discharge of sputum by stimulating the respiratory mucosa.

[0004] When the throat is red and swollen and there is a lot of phlegm, drug treatment is generally used. However, the existing drugs used to treat the throat have relatively single effects, resulting in poor compatibility between various substances, which to a certain extent affects the efficacy of the drugs.

[0005] Therefore, it is necessary to provide a new formula for relieving throat and reducing phlegm and a preparation method thereof to solve the above technical problems. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a formula for relieving throat and reducing phlegm and a preparation method thereof.

[0007] The formula for relieving throat and reducing phlegm provided by the present invention comprises the following components by weight:

[0008] 12-14 parts of Poria cocos, 30-50 parts of Aster, 10-15 parts of Lemongrass seeds, 8-12 parts of Cardamom, 8-12 parts of Ginkgo biloba leaves, 10-25 parts of Pinellia tuber, 10-15 parts of Houttuynia cordata, 12-14 parts of Forsythia suspensa, 10-16 parts of Arctium seeds, 3-7 parts of Water chestnut, 8-10 parts of Jellyfish peel, 10-15 parts of Carrageenan, 10-15 parts of Mustard seeds, 8-10 parts of Loquat, 28-36 parts of Platycodon grandiflorum leaves, 18-25 parts of Belamcanda chinensis, 16-24 parts of Licorice, and 8-15 parts of Eucalyptus leaves;

[0009] The pinellia preparation includes Beijing pinellia, French pinellia and ginger pinellia, and the weight ratio of Beijing pinellia, French pinellia and ginger pinellia is (2-3): (1-2): 3.

[0010] Preferably, the following ingredients are included by weight:

[0011] 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass seeds, 10 parts of Cardamom, 10 parts of Ginkgo leaves, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium seeds, 5 parts of Water Chestnut, 9 parts of Jellyfish peel, 12 parts of Carrageenan, 12 parts of Mustard seeds, 9 parts of Loquat, 31 parts of Platycodon leaves, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus leaves.

[0012] Preferably, the ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is (1-3):3.

[0013] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0014] S1: Weigh the required ingredients in proportion;

[0015] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.2-2 hours, and then decoct for 2-2.5 hours. During the decoction process, stir once every 15 minutes (to prevent the medicinal materials from sticking to the pot). After the decoction is completed, keep the temperature at 75-85°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0016] S3: crushing aster, cardamom, ginkgo leaf, pinellia tuber, houttuynia cordata, forsythia suspensa, and mustard, mixing the crushed ingredients, placing the mixed ingredients into a reflux extraction device, adding 70% ethanol solution as an extraction solvent to the reflux extraction device, performing reflux extraction with ethanol, the reflux extraction temperature is 65-75° C., the time is 2-2.5 h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0017] S4: collecting the water-extracted residue and the alcohol-extracted residue, enzymolyzing the water-extracted residue and the alcohol-extracted residue with β-glucosidase, and filtering to obtain residue enzymolysis liquid and secondary residue;

[0018] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0019] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40° C., adjusting the pH to 4.8, shaking for 1.5-2.5 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0020] S7: crushing the Aster into powder, loading the Aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting extraction conditions, and obtaining an extract;

[0021] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2-3 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0022] S9: Stir and mix the water extraction filtrate and the alcohol extraction filtrate, heat and concentrate to a paste with a relative density of 1.05-1.13, add the residue hydrolyzate, the first hydrolyzate, the second hydrolyzate, the third hydrolyzate and the extract to the paste, mix well, continue to concentrate to a thick paste with a relative density of 1.28-1.32, prepare into granules, and obtain a granular finished product.

[0023] Preferably, in the step S9, the stirring is performed by forward and reverse intermittent stirring, wherein the mixture is stirred clockwise for 1-2 minutes, left to stand for 1 minute, and then stirred counterclockwise for 1-2 minutes, and the rotation speed during clockwise and counterclockwise stirring is 50-100 rpm.

[0024] Preferably, the enzymatic hydrolysis of water extraction residue and alcohol extraction residue by β-glucosidase comprises the following steps:

[0025] S601: using a buffer solution to wash and filter the water extraction residue and the alcohol extraction residue, wherein the buffer solution is an acetic acid-sodium acetate buffer solution with a pH of 5.0;

[0026] S602: mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase;

[0027] S603: Control the temperature at 50°C, adjust the pH to 5.0, oscillate for 1-1.5 hours for enzymolysis, and perform inactivation treatment after the enzymolysis is completed.

[0028] Preferably, the extraction conditions are: pressure of 10-30 MPa, temperature of 40-60° C., and flow rate of 1-3 L / min.

[0029] Preferably, the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase and animal-derived β-glucosidase, wherein the weight ratio of plant-derived β-glucosidase, microbial-derived β-glucosidase and animal-derived β-glucosidase is 1:2.2:1.2.

[0030] Preferably, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase and radish β-glucosidase, the microbial-derived β-glucosidase includes Bacillus subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, and the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and bee larvae β-glucosidase.

[0031] Compared with the related art, the formula for relieving throat and reducing phlegm and the preparation method thereof provided by the present invention have the following beneficial effects:

[0032] 1. When formulating and proportioning, the present invention is compatible according to the principle of "monarch, minister, assistant and messenger", wherein the monarch drugs are aster and platycodon leaf, the minister drugs are pinellia, beam dry and liquorice, the assistant drugs are tuckahoe, scutellaria, houttuynia, forsythia, burdock seed, cardamom, ginkgo, mustard, loquat, jellyfish peel, antler grass and eucalyptus leaves, and the messenger drug is water chestnut. The monarch drugs can moisten the lungs and resolve phlegm, relieve cough and asthma, and relieve sore throat and inflammation. The minister drugs strengthen the effects of resolving phlegm and relieving cough, clearing away heat and detoxicating, and relieving sore throat and swelling. The assistant drugs can relieve dampness and resolve phlegm through the synergistic effect of multiple medicinal materials, and the messenger drugs help other drugs to better play a local role, especially for the symptoms of the throat.

[0033] 2. The present invention adds pinellia ternata, which includes Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata. The weight ratio of Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata is (2-3): (1-2): 3. Each type of pinellia ternata is prepared in a different way, and each type of pinellia ternata has its own unique medicinal properties and effects. This mixture produces a synergistic effect, enhances and balances the efficacy, and improves the effect of the efficacy. Through appropriate formulation and ratio, it has the effect of enhancing immunity.

[0034] 3. The water-extracted residue and the alcohol-extracted residue are hydrolyzed with β-glucosidase to further improve the release of active ingredients. More effective substances in the formula ingredients can be extracted by adding β-glucosidase, and these active ingredients have a promoting effect on improving immunity. Through the action of β-glucosidase, flavonoid glycosides can be converted into new flavonoid derivatives and new derivatives of quercetin. These derivatives may show stronger antioxidant capacity than the parent compound and achieve the effect of immunomodulation. Saponin compounds can be converted into free sapogenins or new sapogenin derivatives after enzymatic hydrolysis. These derivatives are more easily absorbed by the body, thereby improving their pharmacological effects and achieving anti-inflammatory and immune effects. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with the embodiments.

[0036] In the specific implementation process,

[0037] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0038] 12-14 parts of Poria cocos, 30-50 parts of Aster, 10-15 parts of Lemongrass seeds, 8-12 parts of Cardamom, 8-12 parts of Ginkgo biloba leaves, 10-25 parts of Pinellia tuber, 10-15 parts of Houttuynia cordata, 12-14 parts of Forsythia suspensa, 10-16 parts of Arctium seeds, 3-7 parts of Water chestnut, 8-10 parts of Jellyfish peel, 10-15 parts of Carrageenan, 10-15 parts of Mustard seeds, 8-10 parts of Loquat, 28-36 parts of Platycodon grandiflorum leaves, 18-25 parts of Belamcanda chinensis, 16-24 parts of Licorice, and 8-15 parts of Eucalyptus leaves;

[0039] The pinellia tuber agent comprises Beijing pinellia tuber, French pinellia tuber and ginger pinellia tuber, and the weight ratio of Beijing pinellia tuber, French pinellia tuber and ginger pinellia tuber is (2-3): (1-2): 3;

[0040] The following ingredients are included by weight:

[0041] 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass, 10 parts of Cardamom, 10 parts of Ginkgo Leaf, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium, 5 parts of Water Chestnut, 9 parts of Jellyfish, 12 parts of Carrageenan, 12 parts of Mustard, 9 parts of Loquat, 31 parts of Platycodon, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus Leaves;

[0042] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is (1-3):3;

[0043] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0044] S1: Weigh the required ingredients in proportion;

[0045] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.2-2 hours, and then decoct for 2-2.5 hours. During the decoction process, stir once every 15 minutes (to prevent the medicinal materials from sticking to the pot). After the decoction is completed, keep the temperature at 75-85°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0046] S3: crush Aster, cardamom, Ginkgo leaf, Pinellia, Houttuynia, Forsythia, and mustard, mix after crushing, put the mixed components into a reflux extraction device, add 70% ethanol solution as an extraction solvent into the reflux extraction device, perform reflux extraction with ethanol, the reflux extraction temperature is 65-75°C, the time is 2-2.5h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue; (The specific operation process of this step is as follows: put the mixed components into the reflux extractor, the amount of the mixed components is 1 / 3 to 1 / 2 of the capacity of the reflux extractor / 2, add ethanol solvent to the reflux extractor so that the medicinal materials are completely immersed in the solvent, install the reflux extractor on the heater, turn on the heater to make the solvent start to boil, control the heating temperature at 65-75°C, ensure that the solvent vapor condenses into liquid through the condenser, and then refluxes into the extractor to contact with the medicinal materials to achieve extraction. The reflux extraction time is 2-2.5h. During this period, the solvent will continue to evaporate, condense, and reflux, repeatedly extracting the active ingredients in the medicinal materials. After the extraction is completed, stop heating, open the liquid outlet valve of the extractor, and you can get the alcohol extraction filtrate);

[0047] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1-1.5 hours for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue enzymolysis solution and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0048] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0049] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40° C., adjusting the pH to 4.8, shaking for 1.5-2.5 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0050] S7: crushing the aster into powder, loading the aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, the extraction conditions are set to a pressure of 10-30 MPa, a temperature of 40-60° C., and a flow rate of 1-3 L / min, to obtain an extract;

[0051] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2-3 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0052] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate, and stir by positive and negative intermittent stirring, wherein, after stirring clockwise for 1-2 minutes, let stand for 1 minute, and then stir counterclockwise for 1-2 minutes, and the rotation speed during clockwise and counterclockwise stirring is 50-100 rpm. Heat and concentrate to a paste with a relative density of 1.05-1.13, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, and continue to concentrate to a thick paste with a relative density of 1.28-1.32, prepare it into granules, and obtain a granular finished product.

[0053] For the purpose of greater clarity, the present invention is described in detail with reference to the following examples and comparative examples.

[0054] Embodiment 1

[0055] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0056] 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass, 10 parts of Cardamom, 10 parts of Ginkgo Leaf, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium, 5 parts of Water Chestnut, 9 parts of Jellyfish, 12 parts of Carrageenan, 12 parts of Mustard, 9 parts of Loquat, 31 parts of Platycodon, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus Leaves;

[0057] The pinellia ternata agent includes Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata, and the weight ratio of Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata is 2:1:3;

[0058] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0059] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0060] S1: Weigh the required ingredients in proportion;

[0061] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.6 hours, and then decoct for 2.2 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 80°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0062] S3: crush Aster, cardamom, Ginkgo biloba, Pinellia, Houttuynia cordata, Forsythia suspensa, and Mustard, mix the crushed ingredients, put the mixed ingredients into a reflux extraction device, add 70% ethanol solution as an extraction solvent to the reflux extraction device, perform reflux extraction with ethanol, the reflux extraction temperature is 70°C, the time is 2.3h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0063] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1.3 hours for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue enzymolysis solution and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase, and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase, and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase, and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0064] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0065] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40°C, adjusting the pH to 4.8, shaking for 2 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0066] S7: crushing the aster into powder, loading the aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, and setting the extraction conditions to a pressure of 20 MPa, a temperature of 50° C., and a flow rate of 2 L / min to obtain an extract;

[0067] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2.5 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0068] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate, and stir by positive and negative intermittent stirring, wherein, after stirring clockwise for 1.5 minutes, let stand for 1 minute, and then stir counterclockwise for 1.5 minutes, and the rotation speed during clockwise and counterclockwise stirring is 75 rpm. Heat and concentrate to a paste with a relative density of 1.08, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, and continue to concentrate to a thick paste with a relative density of 1.30, prepare it into granules, and obtain a granular finished product.

[0069] Embodiment 2

[0070] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0071] 12 parts of Poria, 30 parts of Aster, 10 parts of Lemongrass, 8 parts of Cardamom, 8 parts of Ginkgo Leaf, 10 parts of Pinellia, 10 parts of Houttuynia, 12 parts of Forsythia, 10 parts of Arctium, 3 parts of Water Chestnut, 8 parts of Jellyfish, 10 parts of Carrageenan, 10 parts of Mustard, 8 parts of Loquat, 28 parts of Platycodon, 18 parts of Belamcanda, 16 parts of Licorice and 8 parts of Eucalyptus Leaves;

[0072] The pinellia ternata agent includes Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata, and the weight ratio of Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata is 2:1:3;

[0073] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0074] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0075] S1: Weigh the required ingredients in proportion;

[0076] S2: Grind Poria cocos, Semen Lepidii, Water Chestnut, Herba Lysimachiae, Platycodon grandiflorum, Belamcanda chinensis, Licorice root and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.2 hours and then decoct for 2 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 75°C for 2 hours and filter to obtain a water extract filtrate and a water extract residue;

[0077] S3: crushing aster, cardamom, ginkgo leaf, pinellia tuber, houttuynia cordata, forsythia suspensa, and mustard, mixing after crushing, putting the mixed components into a reflux extraction device, adding 70% ethanol solution as an extraction solvent to the reflux extraction device, performing reflux extraction with ethanol, the reflux extraction temperature is 65° C., the time is 2 h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0078] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1 hour for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue hydrolyzate and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase, and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase, and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase, and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0079] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0080] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40°C, adjusting the pH to 4.8, shaking for 1.5 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0081] S7: crushing the Aster tataricus into powder, loading the Aster tataricus powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, the extraction conditions are set to a pressure of 10 MPa, a temperature of 40° C., and a flow rate of 1 L / min, and obtaining an extract;

[0082] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0083] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate by positive and negative intermittent stirring, wherein, after stirring clockwise for 1 minute, let stand for 1 minute, and then stir counterclockwise for 1 minute, the rotation speed during clockwise and counterclockwise stirring is 50 rpm, heat and concentrate to a paste with a relative density of 1.05, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, continue to concentrate, concentrate to a thick paste with a relative density of 1.28, prepare into granules, and obtain a granular finished product.

[0084] Embodiment 3

[0085] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0086] 14 parts of Poria, 50 parts of Aster, 15 parts of Lemongrass, 12 parts of Cardamom, 12 parts of Ginkgo Leaf, 25 parts of Pinellia, 15 parts of Houttuynia, 14 parts of Forsythia, 16 parts of Arctium, 7 parts of Water Chestnut, 10 parts of Jellyfish, 15 parts of Carrageenan, 15 parts of Mustard, 10 parts of Loquat, 36 parts of Platycodon, 25 parts of Belamcanda, 24 parts of Licorice and 15 parts of Eucalyptus Leaves;

[0087] The pinellia ternata agent includes Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata, and the weight ratio of Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata is 2:1:3;

[0088] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0089] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0090] S1: Weigh the required ingredients in proportion;

[0091] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice root and Eucalyptus leaves, pour into a container and mix well, add water, soak for 2 hours and then decoct for 2.5 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 85°C for 2 hours and filter to obtain a water extract filtrate and a water extract residue;

[0092] S3: crushing aster, cardamom, ginkgo leaf, pinellia tuber, houttuynia cordata, forsythia suspensa, and mustard, mixing after crushing, putting the mixed components into a reflux extraction device, adding 70% ethanol solution as an extraction solvent to the reflux extraction device, performing reflux extraction with ethanol, the reflux extraction temperature is 75° C., the time is 2.5 h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0093] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1.5 hours for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue enzymolysis solution and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase, and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase, and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase, and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0094] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0095] S6: peeling and core the loquat, cutting into small pieces, and crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40° C., adjusting the pH to 4.8, shaking for 2.5 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0096] S7: crushing the Aster into powder, loading the Aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, the extraction conditions are set to a pressure of 30 MPa, a temperature of 60° C., and a flow rate of 3 L / min, and obtaining an extract;

[0097] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 3 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0098] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate by forward and reverse intermittent stirring, wherein, after stirring clockwise for 2 minutes, let stand for 1 minute, and then stir counterclockwise for 2 minutes, the rotation speed during clockwise and counterclockwise stirring is 100 rpm, heat and concentrate to a paste with a relative density of 1.13, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, continue to concentrate, concentrate to a thick paste with a relative density of 1.32, prepare into granules, and obtain a granular finished product.

[0099] Embodiment 4

[0100] The same formula and preparation method as in Example 1 were followed, except that:

[0101] The pinellia preparation includes Beijing pinellia, French pinellia and ginger pinellia, and the weight ratio of Beijing pinellia, French pinellia and ginger pinellia is 2:2:3.

[0102] Embodiment 5

[0103] The same formula and preparation method as in Example 1 were followed, except that:

[0104] The pinellia preparation includes Beijing pinellia, French pinellia and ginger pinellia, and the weight ratio of Beijing pinellia, French pinellia and ginger pinellia is 3:1:3.

[0105] Embodiment 6

[0106] The same formula and preparation method as in Example 1 were followed, except that:

[0107] The pinellia preparation includes Beijing pinellia, French pinellia and ginger pinellia, and the weight ratio of Beijing pinellia, French pinellia and ginger pinellia is 3:2:3.

[0108] Comparative Example 1

[0109] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0110] 13 parts of Poria cocos, 40 parts of Aster tataricus, 13 parts of Lepidium japonicum, 10 parts of Cardamom, 10 parts of Ginkgo biloba leaves, 13 parts of Houttuynia cordata, 13 parts of Forsythia suspensa, 13 parts of Arctium lappa, 5 parts of Water chestnut, 9 parts of Jellyfish peel, 12 parts of Carrageenan, 12 parts of Mustard seeds, 9 parts of Loquat, 31 parts of Platycodon grandiflorum leaves, 22 parts of Belamcanda chinensis, 20 parts of Licorice and 12 parts of Eucalyptus leaves;

[0111] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0112] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0113] S1: Weigh the required ingredients in proportion;

[0114] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.6 hours, and then decoct for 2.2 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 80°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0115] S3: crush aster, cardamom, ginkgo leaf, houttuynia cordata, forsythia suspensa, and mustard, mix the crushed ingredients, put the mixed ingredients into a reflux extraction device, add 70% ethanol solution as an extraction solvent to the reflux extraction device, perform reflux extraction with ethanol, the reflux extraction temperature is 70°C, the time is 2.3h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0116] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1.3 hours for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue enzymolysis solution and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase, and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase, and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase, and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0117] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0118] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40°C, adjusting the pH to 4.8, shaking for 2 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0119] S7: crushing the aster into powder, loading the aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, and setting the extraction conditions to a pressure of 20 MPa, a temperature of 50° C., and a flow rate of 2 L / min to obtain an extract;

[0120] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2.5 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0121] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate, and stir by positive and negative intermittent stirring, wherein, after stirring clockwise for 1.5 minutes, let stand for 1 minute, and then stir counterclockwise for 1.5 minutes, and the rotation speed during clockwise and counterclockwise stirring is 75 rpm. Heat and concentrate to a paste with a relative density of 1.08, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, and continue to concentrate to a thick paste with a relative density of 1.30, prepare it into granules, and obtain a granular finished product.

[0122] Comparative Example 2

[0123] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0124] 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass, 10 parts of Cardamom, 10 parts of Ginkgo Leaf, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium, 5 parts of Water Chestnut, 9 parts of Jellyfish, 12 parts of Carrageenan, 12 parts of Mustard, 9 parts of Loquat, 31 parts of Platycodon, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus Leaves;

[0125] The pinellia ternata preparation comprises Beijing pinellia ternata and French pinellia ternata, and the weight ratio of Beijing pinellia ternata to French pinellia ternata is 2:1;

[0126] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0127] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0128] S1: Weigh the required ingredients in proportion;

[0129] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.6 hours, and then decoct for 2.2 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 80°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0130] S3: crush Aster, cardamom, Ginkgo biloba, Pinellia, Houttuynia cordata, Forsythia suspensa, and Mustard, mix the crushed ingredients, put the mixed ingredients into a reflux extraction device, add 70% ethanol solution as an extraction solvent to the reflux extraction device, perform reflux extraction with ethanol, the reflux extraction temperature is 70°C, the time is 2.3h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0131] S4: collecting the water-extracted residue and the alcohol-extracted residue, washing the water-extracted residue and the alcohol-extracted residue with a buffer solution, filtering, mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1.3 hours for enzymolysis, inactivating after the enzymolysis, filtering to obtain a residue enzymolysis solution and a secondary residue, wherein the β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase, and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, the microbial-derived β-glucosidase, and the animal-derived β-glucosidase is 1:2.2:1.2, the plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase, and radish β-glucosidase, and the microbial-derived β-glucosidase includes Bacillus subtilis. subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, wherein the animal-derived β-glucosidase includes bovine intestinal mucosal β-glucosidase, rat liver β-glucosidase and honey bee larvae β-glucosidase;

[0132] S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment;

[0133] S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40°C, adjusting the pH to 4.8, shaking for 2 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0134] S7: crushing the aster into powder, loading the aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, and setting the extraction conditions to a pressure of 20 MPa, a temperature of 50° C., and a flow rate of 2 L / min to obtain an extract;

[0135] S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2.5 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment;

[0136] S9: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate, and stir by positive and negative intermittent stirring, wherein, after stirring clockwise for 1.5 minutes, let stand for 1 minute, and then stir counterclockwise for 1.5 minutes, and the rotation speed during clockwise and counterclockwise stirring is 75 rpm. Heat and concentrate to a paste with a relative density of 1.08, add the filter residue enzymatic hydrolyzate, the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, and continue to concentrate to a thick paste with a relative density of 1.30, prepare it into granules, and obtain a granular finished product.

[0137] Comparative Example 3

[0138] The same formula and preparation method as those of Comparative Example 2 were followed, except that:

[0139] The pinellia preparation includes Beijing pinellia and ginger pinellia, and the weight ratio of Beijing pinellia and ginger pinellia is 2:3.

[0140] Comparative Example 4

[0141] The same formula and preparation method as those of Comparative Example 2 were followed, except that:

[0142] The pinellia preparation includes French pinellia and ginger pinellia, and the weight ratio of the French pinellia and ginger pinellia is 1:3.

[0143] Comparative Example 5

[0144] The same formula as in Example 1 was followed, but the preparation method was different from that in Example 1:

[0145] A formula for relieving throat pain and reducing phlegm, comprising the following ingredients by weight:

[0146] 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass, 10 parts of Cardamom, 10 parts of Ginkgo Leaf, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium, 5 parts of Water Chestnut, 9 parts of Jellyfish, 12 parts of Carrageenan, 12 parts of Mustard, 9 parts of Loquat, 31 parts of Platycodon, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus Leaves;

[0147] The pinellia ternata agent includes Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata, and the weight ratio of Beijing pinellia ternata, French pinellia ternata and ginger pinellia ternata is 2:1:3;

[0148] The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is 2:3;

[0149] A method for preparing a formula for relieving throat and reducing phlegm, which is prepared according to the above-mentioned formula for relieving throat and reducing phlegm, comprising the following steps:

[0150] S1: Weigh the required ingredients in proportion;

[0151] S2: Grind Poria cocos, Semen Lemongrass, Water Chestnut, Herba Lycopodii, Platycodon grandiflorum, Belamcanda chinensis, Licorice, and Eucalyptus leaves, pour into a container and mix well, add water, soak for 1.6 hours, and then decoct for 2.2 hours. During the decoction, stir once every 15 minutes. After the decoction is completed, keep the mixture at a constant temperature of 80°C for 2 hours, and filter to obtain a water extract filtrate and a water extract residue;

[0152] S3: crush Aster, cardamom, Ginkgo biloba, Pinellia, Houttuynia cordata, Forsythia suspensa, and Mustard, mix the crushed ingredients, put the mixed ingredients into a reflux extraction device, add 70% ethanol solution as an extraction solvent to the reflux extraction device, perform reflux extraction with ethanol, the reflux extraction temperature is 70°C, the time is 2.3h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue;

[0153] S4: crushing the stag beetle and platycodon grandiflorum leaves, pouring them into a container and mixing them evenly, adding water, then adding water extraction residue and alcohol extraction residue, adding cellulase and pectinase into the container, controlling the temperature at 45°C, adjusting the pH to 5.0, shaking for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuging to obtain a first enzymolysis solution, and performing inactivation treatment;

[0154] S5: peeling and core the loquat, cutting into small pieces, and crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40°C, adjusting the pH to 4.8, shaking for 2 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment;

[0155] S6: crushing the aster into powder, loading the aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting the extraction conditions, and setting the extraction conditions to a pressure of 20 MPa, a temperature of 50° C., and a flow rate of 2 L / min to obtain an extract;

[0156] S7: crush the jellyfish skin, pour it into a container, add water, add cellulase and protease into the container, control the temperature at 43°C, adjust the pH to 4.7, shake for 2.5 hours for enzymolysis, centrifuge to obtain a third enzymolysis solution, and perform inactivation treatment;

[0157] S8: Stir and mix the water-extracted filtrate and the alcohol-extracted filtrate, and stir by positive and negative intermittent stirring, wherein, after stirring clockwise for 1.5 minutes, let stand for 1 minute, and then stir counterclockwise for 1.5 minutes, and the rotation speed during clockwise and counterclockwise stirring is 75 rpm. Heat and concentrate to a paste with a relative density of 1.08, add the first enzymatic hydrolyzate, the second enzymatic hydrolyzate, the third enzymatic hydrolyzate and the extract to the paste, mix well, and continue to concentrate to a thick paste with a relative density of 1.30, prepare into granules, and obtain a granular finished product.

[0158] Experimental Group 1

[0159] 120 experimental mice were divided into 12 groups, each with 10 mice. The finished products prepared according to Examples 1 to 6 and Comparative Examples 1 to 5 were tested. The last group was set as the control group. The control group was gavaged with the same volume of distilled water. Each group of mice was gavaged with 3g crude drug / kg for 5 consecutive days. After the last administration, 3h, the experimental mice were placed in a bell jar and sprayed with 27% ammonia water for 8 seconds at a constant pressure of 53.2KPa to make the mice cough. The cough latency and the number of coughs in 3min of the mice were recorded. The data were analyzed and processed, and the records are shown in the following table:

[0160] Group dose Cough incubation period (s) Coughing times / 3min Blank control group - 53.67±4.32 30.35±3.37 Embodiment 1 3g / kg 87.37±6.34 11.67±2.89 Embodiment 2 3g / kg 79.36±4.31 12.67±3.07 Embodiment 3 3g / kg 81.36±3.69 12.65±2.76 Embodiment 4 3g / kg 82.97±5.98 13.74±3.01 Embodiment 5 3g / kg 80.64±6.43 11.63±3.12 Embodiment 6 3g / kg 85.93±7.97 11.76±2.76 Comparative Example 1 3g / kg 65.36±4.95 20.36±3.61 Comparative Example 2 3g / kg 69.32±3.31 17.63±2.37 Comparative Example 3 3g / kg 70.63±5.34 16.98±3.23 Comparative Example 4 3g / kg 71.91±4.63 16.74±2.79 Comparative Example 5 3g / kg 60.39±5.63 22.34±2.74

[0161] Analyzing the data in the above table, compared with the blank control group, the formulas of each embodiment of the present invention and each comparative example have the effect of relieving cough, and among Examples 1 to 6, the cough latency period of Example 1 is the longest, and the number of coughs within 3 minutes of Example 4 is the least. By comparing the examples with the comparative examples, it can be seen that the present invention achieves better effect by adding the pinellia agent, which includes Beijing pinellia, French pinellia and ginger pinellia, compared with adding only two of them in the comparative example.

[0162] Experimental Group 2

[0163] 120 experimental mice were divided into 12 groups, each with 10 mice. The finished products prepared according to Examples 1 to 6 and Comparative Examples 1 to 5 were tested. The last group was set as the control group. The control group was gavaged with the same volume of normal saline. Each group of mice was gavaged with 3g crude drug / kg for 7 consecutive days. 30 minutes after the last administration, 0.01ml·g-1 of 5% phenol red was injected intraperitoneally. 30 minutes after the injection, the mice were killed by cervical dislocation. The skin in front of the neck was cut open, the tissue around the trachea was separated, and the trachea from the thyroid cartilage to the tracheal bifurcation was cut and placed in a test tube containing 5mL5% NaHCO3 solution. It was shaken for 30 minutes to release the phenol red in the tracheal segment. The OD value of each test tube was measured at a wavelength of 546nm using an ultraviolet-visible spectrophotometer, and the content of phenol red in the secretions of the tracheal segment was calculated. The experimental results were analyzed:

[0164] Group dose Phenol red secretion (μg / mL) Blank control group - 0.76±0.32 Embodiment 1 3g / kg 1.28±0.36 Embodiment 2 3g / kg 1.34±0.39 Embodiment 3 3g / kg 1.32±0.33 Embodiment 4 3g / kg 1.27±0.24 Embodiment 5 3g / kg 1.35±0.33 Embodiment 6 3g / kg 1.38±0.47 Comparative Example 1 3g / kg 0.83±0.28 Comparative Example 2 3g / kg 1.11±0.29 Comparative Example 3 3g / kg 1.15±0.31 Comparative Example 4 3g / kg 1.13±0.37 Comparative Example 5 3g / kg 0.91±0.33

[0165] Analyzing the data in the above table, the phenol red secretion in the embodiments is greater than that in the comparative examples. This shows that the formula and preparation method of the present invention have good expectorant effect, among which the expectorant effect in Example 2 is better.

[0166] Experimental Group 3

[0167] 120 experimental mice were divided into 12 groups, 10 mice in each group, and the pharynx of the experimental mice was sprayed with 2.5% ammonia water 0.3 mL / kg body weight for 7 consecutive days, 3 times a day. After 7 days, the finished products prepared according to Examples 1 to 6 and Comparative Examples 1 to 5 were tested. The last group was set as the control group, and the control group was gavaged with the same volume of normal saline. The mice in each group were gavaged with 3 g of crude drug / kg, and the administration was continued for 7 days. The last administration was 2 hours, and the femoral artery blood of the experimental mice was taken, and then the IL-1β content in the serum was determined, and the records were shown in the following table:

[0168] Group dose IL-1β (ng / mL) Blank control group - 0.336±0.013 Embodiment 1 3g / kg 0.080±0.013 Embodiment 2 3g / kg 0.085±0.012 Embodiment 3 3g / kg 0.078±0.023 Embodiment 4 3g / kg 0.087±0.017 Embodiment 5 3g / kg 0.079±0.018 Embodiment 6 3g / kg 0.080±0.016 Comparative Example 1 3g / kg 0.195±0.013 Comparative Example 2 3g / kg 0.173±0.012 Comparative Example 3 3g / kg 0.170±0.014 Comparative Example 4 3g / kg 0.187±0.015 Comparative Example 5 3g / kg 0.212±0.016

[0169] Analyzing the data in the above table, the IL-1β content in Examples 1 to 6 is lower than that in Comparative Examples 1 to 5, which indicates that the pharyngeal inflammation of the mice using the formulations of Comparative Examples 1 to 5 is more obvious, and the pharyngeal inflammation of the mice using the formulations of Examples 1 to 6 is significantly improved.

[0170] Experimental Group 4

[0171] 120 experimental mice were divided into 12 groups, 10 mice in each group, and the pharynx of the experimental mice was sprayed with 2.5% ammonia water 0.3 mL / kg body weight for 3 consecutive days, once a day. After 3 days, the finished products prepared according to Examples 1 to 6 and Comparative Examples 1 to 5 were tested. The last group was set as the control group, and the control group was gavaged with the same volume of normal saline. The mice in each group were gavaged with 3 g of crude drug / kg for 10 consecutive days. The changes in the pharynx of the experimental mice after administration were scored, and the scoring criteria were as follows:

[0172] 0 points: No abnormality in the pharynx.

[0173] 1 point: Slight redness of the pharynx.

[0174] 2 points: The pharynx is slightly red, swollen and congested, and the mouse occasionally scratches.

[0175] 3 points: The pharynx is obviously red, swollen and congested, and the mouse often scratches.

[0176] 4 points: The pharynx is obviously red, swollen and congested, and there are ulcers.

[0177] The changes in the pharynx of the experimental mice were recorded 2, 4, 6, 8, and 10 days after administration, and the overall performance of each group was scored:

[0178] Group 2 days 4 days 6 days 8 days 10 days Blank control group 4 4 3 3 3 Embodiment 1 3 2 1 0 0 Embodiment 2 3 2 2 0 0 Embodiment 3 3 2 2 0 0 Embodiment 4 3 2 1 0 0 Embodiment 5 3 2 2 0 0 Embodiment 6 3 2 1 0 0 Comparative Example 1 4 3 3 2 1 Comparative Example 2 3 3 2 1 0 Comparative Example 3 3 3 2 2 0 Comparative Example 4 3 3 2 1 0 Comparative Example 5 4 3 3 2 1

[0179] Analysis of the data in the above table shows that when the finished product provided by the present invention is used to treat mice with pharyngeal inflammation, the pharynx can be normal on the 8th day of treatment. Among them, Example 1, Example 4 and Example 6 can all achieve a state of slight redness of the pharynx on the 6th day. In the comparative examples, there is no Pinellia agent in Comparative Example 1, and the mice recover relatively slowly. Comparative Example 5 omits the step of "collecting the water extraction residue and the alcohol extraction residue, washing the water extraction residue and the alcohol extraction residue with a buffer solution, filtering, mixing the washed water extraction residue and the alcohol extraction residue, adding water, and then adding β-glucosidase, controlling the temperature at 50°C, adjusting the pH to 5.0, oscillating for 1.3 hours for enzymolysis, and after the enzymolysis is completed, inactivating the residue, filtering to obtain the residue enzymolysis liquid and the secondary residue", and the mice also recover relatively slowly. In Comparative Examples 2, 3 and 4, two of Beijing Pinellia, French Pinellia and Jiang Pinellia are used, and the effect is obviously not as good as when Beijing Pinellia, French Pinellia and Jiang Pinellia are all used.

[0180] Experimental Group 5

[0181] The experimental mice in experimental group 4 that did not recover after 10 days were given the same dosage as that in experimental group 4 until the mice recovered. The mice in experimental group 4 that recovered were fed normally for 10 days. Then, the inactivated influenza virus used by mice was selected, and the influenza vaccine was subcutaneously injected (0.1 ml / mouse) into each group of mice. The behavioral state of the mice was observed 5 days, 10 days, and 15 days after vaccination. The mental state was scored from good to bad as 1, 2, 3, and 4, respectively. The overall changes of each group are recorded in the following table:

[0182] Group 5 days later 10 days 15 days Blank control group 3 4 4 Embodiment 1 1 1 2 Embodiment 2 1 1 2 Embodiment 3 1 2 2 Embodiment 4 1 1 2 Embodiment 5 1 2 2 Embodiment 6 1 1 2 Comparative Example 1 2 3 4 Comparative Example 2 2 3 3 Comparative Example 3 2 3 3 Comparative Example 4 2 2 3 Comparative Example 5 2 3 4

[0183] Analysis of the above table shows that when the experimental mice first develop pharyngeal inflammation, timely administration of the finished product provided by the present invention can not only accelerate the recovery speed of the health status of the experimental mice, but also after the recovery of each group of mice, the experimental mice to which the finished product provided by the present invention is timely administered are in good mental state after being inoculated with the virus. This shows that the formulation and method adopted by the present invention can improve the immunity of mice to a certain extent. In comparative example 1, two of Beijing Pinellia, French Pinellia and Jiang Pinellia are used. According to the data in the table, the simultaneous use of Beijing Pinellia, French Pinellia and Jiang Pinellia has the effect of enhancing immunity. In comparative example 5, the step of "collecting the water extraction residue and the alcohol extraction residue, washing the water extraction residue and the alcohol extraction residue with a buffer solution, filtering, mixing the washed water extraction residue and the alcohol extraction residue, adding water, and then adding β- β-glucosidase, control the temperature at 50°C, adjust the pH to 5.0, oscillate for 1.3h for enzymolysis, after the enzymolysis is completed, inactivate, filter to obtain the residue enzymolysis liquid and the secondary residue", the mice in comparative example 5 are also sensitive to ammonia water, which shows that during the preparation of this formula, more effective substances in the ingredients can be extracted by adding β-glucosidase, and these effective ingredients have a promoting effect on improving immunity. Through the action of β-glucosidase, flavonoid glycosides can be converted into new flavonoid derivatives, such as new derivatives of quercetin, which may show stronger antioxidant capacity than the parent compound and achieve the effect of immunoregulation. Saponin compounds can be converted into free sapogenin or new sapogenin derivatives after enzymolysis, and these derivatives are more easily absorbed by the body, thereby improving their pharmacological effects and achieving anti-inflammatory and immune effects.

[0184] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the specification of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A formula for relieving throat pain and reducing phlegm, characterized in that: By weight Includes the following ingredients: 12-14 parts of Poria cocos, 30-50 parts of Aster, 10-15 parts of Lemongrass seeds, 8-12 parts of Cardamom, 8-12 parts of Ginkgo biloba leaves, 10-25 parts of Pinellia tuber, 10-15 parts of Houttuynia cordata, 12-14 parts of Forsythia suspensa, 10-16 parts of Arctium seeds, 3-7 parts of Water chestnut, 8-10 parts of Jellyfish peel, 10-15 parts of Carrageenan, 10-15 parts of Mustard seeds, 8-10 parts of Loquat, 28-36 parts of Platycodon grandiflorum leaves, 18-25 parts of Belamcanda chinensis, 16-24 parts of Licorice, and 8-15 parts of Eucalyptus leaves; The pinellia preparation includes Beijing pinellia, French pinellia and ginger pinellia, and the weight ratio of Beijing pinellia, French pinellia and ginger pinellia is (2-3): (1-2):

3.

2. The formula for relieving throat pain and reducing phlegm according to claim 1, characterized in that: By weight Includes the following ingredients: 13 parts of Poria, 40 parts of Aster, 13 parts of Lemongrass seeds, 10 parts of Cardamom, 10 parts of Ginkgo leaves, 18 parts of Pinellia, 13 parts of Houttuynia, 13 parts of Forsythia, 13 parts of Arctium seeds, 5 parts of Water Chestnut, 9 parts of Jellyfish peel, 12 parts of Carrageenan, 12 parts of Mustard seeds, 9 parts of Loquat, 31 parts of Platycodon leaves, 22 parts of Belamcanda, 20 parts of Licorice and 12 parts of Eucalyptus leaves.

3. The formula for relieving throat pain and reducing phlegm according to claim 1, characterized in that: The ginkgo leaves include male ginkgo leaves and female ginkgo leaves, and the weight ratio of the male ginkgo leaves to the female ginkgo leaves is (1-3):

3.

4. A method for preparing a formula for relieving throat and reducing phlegm, comprising preparing the formula for relieving throat and reducing phlegm according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1: Weigh the required ingredients in proportion; S2: grind Poria cocos, scutellaria baicalensis, water chestnut, antler herb, platycodon grandiflorum leaf, belamcanda chinensis, liquorice and eucalyptus leaves, pour into a container and mix well, add water, soak for 1.2-2h and then decoct for 2-2.5h. During the decoction, stir every 15min. After the decoction is completed, keep the mixture at a constant temperature of 75-85℃ for 2h, and filter to obtain a water extract filtrate and a water extract residue; S3: crushing aster, cardamom, ginkgo leaf, pinellia tuber, houttuynia cordata, forsythia suspensa, and mustard, mixing the crushed ingredients, placing the mixed ingredients into a reflux extraction device, adding 70% ethanol solution as an extraction solvent to the reflux extraction device, performing reflux extraction with ethanol, the reflux extraction temperature is 65-75° C., the time is 2-2.5 h, and an alcohol extraction filtrate is obtained. The remaining substance in the reflux extraction device is an alcohol extraction residue; S4: collecting the water-extracted residue and the alcohol-extracted residue, enzymolyzing the water-extracted residue and the alcohol-extracted residue with β-glucosidase, and filtering to obtain residue enzymolysis liquid and secondary residue; S5: crush the stag beetle and platycodon grandiflorum leaves, pour them into a container and mix them evenly, add water, then add the secondary filter residue, add cellulase and pectinase into the container, control the temperature at 45°C, adjust the pH to 5.0, shake for 2-3 hours for enzymolysis, and after the enzymolysis is completed, centrifuge to obtain the first enzymolysis solution, and perform inactivation treatment; S6: peeling and core the loquat, cutting into small pieces, crushing the burdock, putting the processed loquat and burdock into a container, adding water, adding cellulase, pectinase and hemicellulase, controlling the temperature at 40° C., adjusting the pH to 4.8, shaking for 1.5-2.5 hours for enzymolysis, centrifuging to obtain a second enzymolysis solution, and performing inactivation treatment; S7: crushing the Aster into powder, loading the Aster powder into a supercritical fluid extraction device, using supercritical carbon dioxide as a solvent, adjusting extraction conditions, and obtaining an extract; S8: crushing the jellyfish skin, pouring it into a container, adding water, adding cellulase and protease into the container, controlling the temperature at 43°C, adjusting the pH to 4.7, shaking for 2-3 hours for enzymolysis, centrifuging, obtaining a third enzymolysis solution, and performing inactivation treatment; S9: Stir and mix the water extraction filtrate and the alcohol extraction filtrate, heat and concentrate to a paste with a relative density of 1.05-1.13, add the residue hydrolyzate, the first hydrolyzate, the second hydrolyzate, the third hydrolyzate and the extract to the paste, mix well, continue to concentrate to a thick paste with a relative density of 1.28-1.32, prepare into granules, and obtain a granular finished product.

5. The method for preparing the formula for relieving throat pain and reducing phlegm according to claim 4, characterized in that: In the step S9, the stirring is performed by forward and reverse intermittent stirring, wherein the mixture is stirred clockwise for 1-2 minutes, left to stand for 1 minute, and then stirred counterclockwise for 1-2 minutes. The rotation speeds during the clockwise and counterclockwise stirring are both 50-100 rpm.

6. The method for preparing the formula for relieving throat pain and reducing phlegm according to claim 4, characterized in that: The enzymatic hydrolysis of the water extraction residue and the alcohol extraction residue by β-glucosidase comprises the following steps: S601: using a buffer solution to wash and filter the water extraction residue and the alcohol extraction residue; S602: mixing the washed water-extracted residue and the alcohol-extracted residue, adding water, and then adding β-glucosidase; S603: Control the temperature at 50°C, adjust the pH to 5.0, oscillate for 1-1.5 hours for enzymolysis, and perform inactivation treatment after the enzymolysis is completed.

7. The method for preparing the formula for relieving throat pain and reducing phlegm according to claim 4, characterized in that: The extraction conditions are: pressure of 10-30 MPa, temperature of 40-60° C., and flow rate of 1-3 L / min.

8. The method for preparing the formula for relieving throat pain and reducing phlegm according to claim 4, characterized in that: The β-glucosidase includes plant-derived β-glucosidase, microbial-derived β-glucosidase and animal-derived β-glucosidase, wherein the weight ratio of the plant-derived β-glucosidase, microbial-derived β-glucosidase and animal-derived β-glucosidase is 1:2.2:1.

2.

9. The method for preparing the formula for relieving throat pain and reducing phlegm according to claim 8, characterized in that: The plant-derived β-glucosidase includes soybean β-glucosidase, grape β-glucosidase and radish β-glucosidase, the microbial-derived β-glucosidase includes Bacillus subtilis β-glucosidase and thermophilic Chaetomium β-glucosidase, and the animal-derived β-glucosidase includes bovine small intestinal mucosal β-glucosidase, rat liver β-glucosidase and bee larvae β-glucosidase.