Anti-allergy repairing composition as well as product and application thereof

Abstract

The invention discloses an anti-allergy repairing composition as well as a product and application thereof. The anti-allergy repairing composition provided by the invention is prepared from alligator oil, tea polyphenol, centella asiatica, cetostearyl alcohol and amino acid. After the alligator oil is compounded with the other four components, the anti-allergy repair effect is very good, the repair of damaged cuticle can be accelerated, and the water content of skin can be increased, so that the barrier function of the skin is improved; the components have a remarkable synergistic effect, the effect of the composition is remarkably superior to that of single use of the components or compound use of any components, and the composition has a relatively good anti-allergy repairing effect. Meanwhile, the extraction method of the alligator oil is further optimized, and the alligator oil extracted by adopting a specific eutectic solvent has a relatively high extraction rate and high content of effective components, and has a better effect when being used in an anti-allergy repair combination. The invention provides a safer and more efficient anti-allergy repair product for sensitive skin and barrier damage, and also provides more choices for alligator oil product development.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and more specifically, relates to an anti-allergic repair composition and a product and application thereof. Background Art

[0002] Sensitive skin is a problematic skin, and any skin type may have sensitive skin. When the stratum corneum of the skin is damaged and becomes sensitive, its defense against the outside world is reduced. When it is stimulated by the outside world, symptoms such as redness, fever, itching, and stinging will appear. In severe cases, redness, swelling, and rashes will appear. When the skin barrier is damaged, inflammation will also occur. When the inflammation fades, it is easy to leave marks or spots. At present, the repair of sensitive skin requires protecting the skin barrier, avoiding irritation, and not using irritating skin care products and cosmetics that cause skin allergies. External red and blue light irradiation can improve the barrier damage of sensitive skin, reduce local inflammation of the skin, improve the redness, and speed up the repair of the skin barrier; but this method requires special instruments and equipment and cannot be widely used. There are medical skin care products specifically for sensitive skin and damaged barriers. They are less irritating to the skin, can help the skin repair itself, do not contain fragrances and preservatives, and are safe and reliable, but there are currently few such products.

[0003] In the field of skin care products, people have never stopped exploring natural and efficient raw materials. Crocodile oil, as a natural substance with anti-allergic and repairing effects, has shown great potential in the fields of skin care and medicine, and has attracted much attention in recent years. Crocodile oil contains unsaturated fatty acids, vitamin E, etc., and has anti-inflammatory and anti-wrinkle effects, promotes skin cell regeneration, etc. It can effectively relieve skin allergy symptoms, repair damaged skin barriers, and improve skin immunity and resistance. Crocodile oil has excellent permeability. After contacting the skin, it can penetrate deep into the skin tissue and form a protective film on the skin surface. This protective film can prevent direct damage to the skin by external factors such as ultraviolet rays, improve skin microcirculation, and delay the aging process of the skin. This deep penetration characteristic enables the vitamin E and unsaturated fatty acids in crocodile oil to directly act on the collagen and elastic fibers of the skin, promote their synthesis and repair, and enhance the supporting structure of the skin, thereby effectively reducing skin itching, redness, dryness, acne, and tingling, providing scientific help for skin anti-allergic repair. Therefore, the application of crocodile oil in the preparation of anti-allergic repair essence oil has broad prospects.

[0004] In order to prevent and alleviate local discomfort of the skin, enhance the skin's resistance, reduce the stimulation of the skin by external substances, and improve the condition of sensitive skin, it is urgent to develop more anti-allergic repair products that are specifically targeted at sensitive skin and damaged skin barriers and are safer and more efficient. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of existing skin care products for sensitive skin and damaged skin barriers, and to provide an anti-allergic repair composition and its product and application.

[0006] The first object of the present invention is to provide an anti-allergic repair composition.

[0007] The second object of the present invention is to provide application of the anti-allergic repair composition.

[0008] The third object of the present invention is to provide an anti-allergic repair product.

[0009] The fourth object of the present invention is to provide a method for preparing an anti-allergic repair product.

[0010] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0011] The invention provides an anti-allergic repair composition, comprising the following components in a mass ratio: crocodile oil: tea polyphenols: centella asiatica: cetearyl alcohol: amino acid=(10-40):(2-6):(3-9):(4-8):(5-15).

[0012] The anti-allergic repair composition provided by the present invention includes crocodile oil, tea polyphenols, Centella asiatica, cetearyl alcohol, and amino acids. After crocodile oil is compounded with the other four components, the prepared anti-allergic repair composition has the function of activating cell regeneration, can decompose skin aging damaged cells, accelerate metabolism, and promote skin cells to accelerate the alternation of old and new cells to achieve the effect of anti-allergic repair, can accelerate the repair of damaged stratum corneum and increase skin water content, thereby improving the barrier function of the skin; and there is a significant synergistic effect between the components, and its effect is significantly better than that of each component used alone or used in combination with any of several components, and can significantly improve the anti-allergic repair effect, and has a good anti-allergic repair function. The present invention provides a safer and more efficient anti-allergic repair product for sensitive skin and damaged barriers, and also provides more options for the development of crocodile oil products.

[0013] Preferably, the components are included in the following mass ratio: crocodile oil: tea polyphenols: Centella asiatica: cetearyl alcohol: amino acid = (15-30): (3-5): (4-8): (5-7): (10-15).

[0014] More preferably, the components are included in the following mass ratio: crocodile oil: tea polyphenols: Centella asiatica: cetearyl alcohol: amino acid = 25:3:8:7:10.

[0015] Traditional crocodile oil extraction methods include organic solvent extraction, squeezing and extraction. On the one hand, these methods are complicated to operate, easy to have residual organic solvents, and the removal process is also relatively complicated; on the other hand, the extraction rate of crocodile oil is low, and the effective ingredients in crocodile fat cannot be fully extracted to meet the high demand for crocodile oil today, resulting in waste of resources, which greatly limits the application of crocodile oil in skin care products. Therefore, the present invention further aims at the defect of low extraction rate of crocodile oil in traditional methods, and adopts low eutectic solvent (DES) to optimize ultrasonic extraction of crocodile oil, which has better extraction ability for crocodile oil, and the extraction rate is greater than 70%, and the content of its effective ingredient EPA is greater than 0.5%, and the content of DHA is greater than 1.43%, which is significantly better than the traditional crocodile oil extraction method-supercritical fluid extraction method and dry boiling method. And by optimizing the crocodile oil extraction process parameters, the efficient extraction of crocodile oil is achieved, and the extracted crocodile oil is used in the anti-allergic repair composition, which can give full play to the anti-inflammatory and anti-allergic repair effect of crocodile oil, further improve the effect of the anti-allergic repair composition, effectively improve the use efficiency of crocodile oil, and be green and environmentally friendly.

[0016] Preferably, the crocodile oil is obtained by extraction using a low eutectic solvent, and the specific method is: crocodile fat and the low eutectic solvent are mixed, ultrasonic extraction is performed, and the crocodile oil is separated after centrifugation.

[0017] More preferably, the specific extraction steps of extracting crocodile oil using a low eutectic solvent are: adding a low eutectic solvent to crocodile fat at a solid-liquid ratio of 1:30-50 g / mL, and extracting for 20-90 minutes under ultrasonic conditions at a temperature of 30-80°C and an ultrasonic power of 80-100 W.

[0018] Furthermore, the low eutectic solvent is prepared from hydrogen bond acceptor benzalkonium chloride and hydrogen bond co-acting lauryl alcohol.

[0019] Preferably, the molar ratio of benzalkonium chloride to lauryl alcohol is 1-3:1.

[0020] More preferably, the water content of the deep eutectic solvent is 50-60%.

[0021] The present invention provides application of the composition in repairing sensitive skin or skin barrier.

[0022] The present invention provides application of the composition in preparing an anti-allergic repair product.

[0023] The invention also provides an anti-allergic repair product containing the composition.

[0024] Preferably, the product is an anti-allergic repair essential oil.

[0025] The present invention also provides a preparation method of the anti-allergic repair essential oil: preheat crocodile oil and add water and tea polyphenols, stir, heat up to 85-90°C, and homogenize; then add amino acids, homogenize, cool to 65-70°C, add Centella asiatica and cetearyl alcohol, homogenize, cool in vacuum, and discharge to obtain the anti-allergic repair essential oil.

[0026] More preferably, the mass ratio of crocodile oil to water is (10-30):(30-60).

[0027] Furthermore, the preparation method of the anti-allergic repairing essential oil is as follows:

[0028] (1) adding water and tea polyphenols to crocodile oil, stirring, heating to 85-90° C., homogenizing, rotating at 2500-2800 r / min, for 1-3 minutes, and keeping warm for 10-15 minutes;

[0029] (2) Add amino acids and homogenize at a speed of 2500-2800 r / min for 1-3 minutes, and cool to 65-70°C;

[0030] (3) Finally, add Centella asiatica and cetearyl alcohol, homogenize at a speed of 2500-2800 r / min for 1-3 minutes, cool under vacuum, and discharge.

[0031] Preferably, the anti-allergic repair product also contains other auxiliary ingredients such as moisturizing and antioxidant.

[0032] The present invention has the following beneficial effects:

[0033] The anti-allergic repair composition provided by the present invention includes crocodile oil, tea polyphenols, Centella asiatica, cetearyl alcohol, and amino acids. After compounding crocodile oil with the other four components, it has a good anti-allergic repair effect, can accelerate the repair of damaged stratum corneum and increase skin moisture content, thereby improving the barrier function of the skin; it has the function of activating cell regeneration, can decompose skin aging damaged cells, accelerate metabolism, and promote skin cells to accelerate the alternation of old and new cells to achieve the effect of anti-allergic repair. And there is a significant synergistic effect between the components, and its effect is significantly better than the use of each component alone or the use of any several components in combination, and it has a good anti-allergic repair function after being applied to the skin. The present invention provides a safer and more efficient anti-allergic repair product for sensitive skin and damaged barriers, and also provides more options for the development of crocodile oil products.

[0034] At the same time, the present invention further optimizes the extraction method of crocodile oil, showing that the crocodile oil extracted by using a low eutectic solvent has a higher extraction rate, a high content of effective ingredients, and an extraction effect that is better than the traditional extraction method. Using it in an anti-allergic repair essence product can further enhance the effect of its composition. The present invention uses a low eutectic solvent to extract crocodile oil, which not only improves the extraction rate and effective ingredient content of crocodile oil, but also DES, as a new type of green solvent, has the advantages of being non-toxic, simple to prepare, easy to degrade, and recyclable compared to common ionic liquids. It is safe and environmentally friendly, and provides a safer and more reliable method for extracting crocodile oil. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a bar graph of hyaluronic acid inhibition rate of different combinations.

[0036] Figure 2 It is a bar graph of -OH clearance rate of different combinations.

[0037] Figure 3 The bar graph shows the TEWL value results for different combinations.

[0038] Figure 4 The following is a bar graph showing skin moisture content for different combinations. DETAILED DESCRIPTION

[0039] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0040] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0041] Embodiment 1 Extraction of crocodile oil

[0042] In order to increase the content of effective ingredients in crocodile oil, improve the yield and effect of crocodile oil, crocodile oil was extracted by deep eutectic solvent (DES), the extraction method of crocodile oil was optimized, and the crocodile oil prepared was used in the preparation of subsequent anti-allergic repair essence composition. The specific extraction method is:

[0043] (1) Preparation of DES: accurately weigh hydrogen bond acceptor (HBA) benzalkonium chloride and hydrogen bond donor (HBD) lauryl alcohol in a molar ratio of 3:1, place in a round-bottom flask, add 50% water, stir in a constant temperature water bath at 80°C for 2 h until a uniform transparent liquid is formed, and prepare a DES solution;

[0044] (2) Extraction of crocodile oil: crocodile fat and DES solution were mixed at a solid-liquid ratio of 1:40, and treated with ultrasound at a temperature of 80°C for 90 min with an ultrasound power of 100 W. The mixture was centrifuged in a TDZ5WS centrifuge for 8

[0045] min, layered DES and crocodile oil were obtained, and crocodile oil was obtained after separation.

[0046] At the same time, different extraction condition parameters were set to determine the best conditions for extracting crocodile oil and optimize the crocodile oil extraction method. The specific condition parameters are shown in Table 1, and the extraction rate of the crocodile oil prepared was calculated.

[0047] And calculate the content of polyunsaturated fatty acids EPA and DHA.

[0048] The specific calculation formula is:

[0049] Extraction rate = crocodile oil mass / crocodile fat mass × 100%

[0050] The results are shown in Table 1, which show that the use of low eutectic solvents for ultrasonic extraction of crocodile oil has a higher crocodile oil extraction rate, up to 82%; and benzalkonium chloride is used as HBA, which has a lone pair of electrons on the nitrogen atom;

[0051] Lauryl alcohol, as HBD, contains hydroxyl group -OH, which can provide hydrogen atoms to form hydrogen bonds with nitrogen ions in benzalkonium chloride. The formation of hydrogen bonds creates a strong interaction between the two, firmly binding crocodile oil in DES. The molecular structure of benzalkonium chloride and lauryl alcohol is mainly composed of non-polar hydrocarbon chains, and the non-polar part accounts for a relatively large proportion.

[0052] The DES formed is hydrophobic as a whole, and pure crocodile oil can be extracted by ultrasonic extraction. 15 has good extraction ability for crocodile oil. Further, by optimizing the crocodile oil extraction process parameters, the crocodile

[0053] Efficient extraction of oil. Compared with the common ionic liquid extraction of crocodile oil, DES has the advantages of non-toxicity, simple preparation, easy degradation, and recyclability, which brings new ideas for the extraction of crocodile oil.

[0054] Table 1 Effect of different extraction methods on crocodile oil extraction rate

[0055] Case DES1 DES2 DES3 DES4 DES5 DES6 DES7 HBA:HBD molar ratio 3：1 1：1 3：1 3：1 3：1 3：1 3：1 Solid-liquid ratio / g / mL 1：40 1：40 1：30 1：40 1：40 1：40 1：40 DES moisture content / % 50 50 50 60 50 50 50 Temperature / ℃ 80 80 80 80 80 50 80 Ultrasonic time / min 90 90 90 90 90 90 60 Ultrasonic power / W 100 100 100 100 90 100 100 Crocodile oil extraction rate / % 82 78 75 76 82 70 70 EPA content / % 0.59 0.53 0.50 0.56 0.50 0.54 0.58 DHA content / % 1.56 1.53 1.59 1.58 1.59 1.43 1.45

[0056] At the same time, compared with the traditional crocodile oil extraction method, the traditional extraction method of comparative example 4-5, the dry method boiling method, and the supercritical fluid extraction method were respectively used for extraction comparison. The results are shown in Table 2, which shows that compared with the crocodile oil extracted by the traditional method, the extraction rate of the crocodile oil extracted by the low eutectic solution is significantly higher, and the content of the effective ingredient unsaturated fatty acids is also increased.

[0057] Table 2 Effect of traditional extraction methods on crocodile oil extraction rate

[0058] Extraction method Extraction rate / % EPA content / % DHA content / % Deep eutectic solvent extraction 82.00 0.59 1.56 Supercritical Fluid Extraction 30.60 0.35 0.19 Dry cooking method 60.85 0.45 0.23

[0059] Example 2 Anti-allergic repair essence oil

[0060] The anti-allergic repair essential oil is prepared by weight in grams from 25 g crocodile oil, 3 g tea polyphenols, 8 g Centella asiatica, 7 g cetearyl alcohol, 10 g amino acids, and 55 g water.

[0061] Place crocodile oil (crocodile oil prepared by DES1 group in Example 1) in a water bath, add water and tea polyphenols, stir, and heat to 90°C, start homogenization, speed at 2500r / min, time for 3 minutes, and keep warm for 10 minutes; then add amino acids, start homogenization, speed at 2500r / min, time for 3 minutes, and cool to 65°C; then add Centella asiatica and cetearyl alcohol, start homogenization, speed at 2500r / min, time for 3 minutes, vacuum cool, and discharge to obtain anti-allergic repair essential oil.

[0062] Example 3 Anti-allergic repair essence oil

[0063] The anti-allergic repair essential oil is prepared by weight in grams from 30 g crocodile oil, 5 g tea polyphenols, 5 g Centella asiatica, 5 g cetearyl alcohol, 13 g amino acids, and 60 g water.

[0064] Place crocodile oil (crocodile oil prepared by DES1 group in Example 1) in a water bath, add water and tea polyphenols, stir, and heat to 90°C, start homogenization, speed at 2500r / min, time for 3 minutes, and keep warm for 10 minutes; then add amino acids, start homogenization, speed at 2500r / min, time for 3 minutes, and cool to 65°C; then add Centella asiatica and cetearyl alcohol, start homogenization, speed at 2500r / min, time for 3 minutes, vacuum cool, and discharge to obtain anti-allergic repair essential oil.

[0065] Example 4 Anti-allergic repair essence oil

[0066] The anti-allergic repair essential oil is prepared by weight in grams from 15 g crocodile oil, 3 g tea polyphenols, 4 g Centella asiatica, 5 g cetearyl alcohol, 10 g amino acids, and 35 g water.

[0067] Place crocodile oil (crocodile oil prepared by DES1 group in Example 1) in a water bath, add water and tea polyphenols, stir, and heat to 90°C, start homogenization, speed at 2500r / min, time for 3 minutes, and keep warm for 10 minutes; add amino acids, start homogenization, speed at 2500r / min, time for 3 minutes, and cool to 65°C; add Centella asiatica and cetearyl alcohol, start homogenization, speed at 2500r / min, time for 3 minutes, vacuum cool, and discharge to obtain anti-allergic repair essential oil.

[0068] Example 5 Synergistic Effect of Anti-allergic Repair Composition

[0069] At the same time, a variety of anti-allergic repair essence oil combinations with different components and contents were studied and prepared. The specific components and their contents are shown in Table 3 below. Different combinations were prepared according to the method of Example 2, and the skin moisture content and TEWL value were measured. During the measurement, the dosage concentration of each group was controlled to be the same.

[0070] The results are shown in Table 3 below, which shows that the effect of crocodile oil alone combined with tea polyphenols, Centella asiatica, cetearyl alcohol or amino acids is not significantly improved, while the effects of crocodile oil and any other three components (combinations 9-10) on skin moisture content and TEWL value are better than those of crocodile oil and any other two components (combinations 6-8), but are not as significant as the effect of crocodile oil combined with four compositions in Example 2; the effect of combination 2-3 and combination 11-12 in the table cannot reach the effect of Example 2 after simple superposition; the effect of the composition of Example 2 is significantly better than the use of each component alone or the combination of any two or three components, and there is a very significant synergistic effect between the four components, which can significantly increase skin moisture content and reduce TEWL value, and can be better used for skin repair. Crocodile oil, tea polyphenols, Centella asiatica, cetearyl alcohol and amino acids have a synergistic effect after compounding.

[0071] Table 3 Skin moisture content and TEWL value of different anti-allergic repair essence oil combinations

[0072]

[0073]

[0074] Example 6 Anti-allergic repair essence oil

[0075] The formula of anti-allergic repair essential oil is the same as that of Example 2.

[0076] Place crocodile oil (crocodile oil prepared by DES1 group in Example 1) in a water bath, add water and tea polyphenols, stir, and heat to 85°C, start homogenization, speed at 2200r / min, time for 5 minutes, and keep warm for 10 minutes; add amino acids, start homogenization, speed at 2200r / min, time for 5 minutes, and cool to 65°C; add Centella asiatica and cetearyl alcohol, start homogenization, speed at 2200r / min, time for 5 minutes, vacuum cool, and discharge to obtain anti-allergic repair essential oil.

[0077] Example 7 Anti-allergic repair essence oil

[0078] The formula of anti-allergic repair essential oil is the same as that of Example 2.

[0079] Place crocodile oil (crocodile oil prepared by DES1 group in Example 1) in a water bath, add water and tea polyphenols, stir, and heat to 90°C, start homogenization, speed at 2500r / min, time for 3 minutes, and keep warm for 8 minutes; add amino acids, start homogenization, speed at 2500r / min, time for 3 minutes, and cool to 70°C; add Centella asiatica and cetearyl alcohol, start homogenization, speed at 2500r / min, time for 3 minutes, vacuum cool, and discharge to obtain anti-allergic repair essential oil.

[0080] Example 8 Anti-allergic repair essence oil

[0081] The anti-allergic repair essential oil includes, by weight: 25 g crocodile oil (crocodile oil prepared by the traditional method of Comparative Example 4), 3 g tea polyphenols, 8 g Centella asiatica, 7 g cetearyl alcohol, 10 g amino acid, and 55 g water. The preparation method is the same as that of Example 2.

[0082] Comparative Example 1

[0083] The anti-allergic repair essential oil component and its preparation method are the same as those in Example 2, except that crocodile oil is replaced with carrot seed essential oil.

[0084] Comparative Example 2

[0085] The anti-allergic repair essential oil component and its preparation method are the same as those in Example 2, with the only difference being that tea polyphenols and Centella asiatica are replaced by rosemary extract and chamomile.

[0086] Comparative Example 3

[0087] The anti-allergic repair essential oil component and its preparation method are the same as those in Example 1, with the only difference being that cetearyl alcohol and amino acids are replaced with 1,3-propylene glycol and ceramide.

[0088] Comparative Example 4 Traditional crocodile oil extraction (dry boiling extraction method)

[0089] Take a certain amount of crocodile fat, wash it and put it in a stainless steel pot, heat it to 100℃, simmer it slowly, add white clay to decolorize it, make the oil come out completely and remove the residue to get the primary oil, weigh 5g of the primary oil in a 50mL three-necked flask, add 1.2g KOH, 2.5mL water and 15mL anhydrous ethanol, put it in a 60℃ water bath under N 2 Stir and reflux for 1h under protection. After saponification is complete, cool rapidly in an ice bath, add 5mL of water and 20mL of petroleum ether, extract twice, remove the petroleum ether layer, and the lower layer of the extract, i.e. the water layer, is acidified to pH 1-2 with 3mol of HCl, and then extracted twice with 10mL of petroleum ether, dried and filtered. The filtrate is placed in a crucible and evaporated to dryness in a 60°C water bath to obtain crocodile oil.

[0090] Comparative Example 5 Preparation of crocodile oil by supercritical fluid extraction

[0091] Take a certain amount of crocodile fat and soak it in water for 60 minutes. After soaking, absorb the moisture on the surface of the crocodile fat and drain it. Then put the crocodile fat and supercritical carbon dioxide into the extractor, set the extraction pressure to 25MPa and the temperature to 50℃, and separate after 5h of extraction. The separation pressure is 5MPa and the temperature is 40℃, and the separated crocodile oil is collected.

[0092] Test Example 1 Anti-allergic efficacy test - inhibition of hyaluronidase

[0093] Hyaluronidase (HAase) is a participant in type I allergic reactions and immediate allergic reactions. Studies have shown that hyaluronidase is strongly correlated with inflammation and allergies, and many anti-allergic drugs have a strong inhibitory effect on hyaluronidase activity. The anti-allergic efficacy of Examples 1-4, 6-8 and Comparative Examples 1-5 was determined by the hyaluronidase in vitro inhibition method (wherein Example 1 measured the crocodile oil extracted from the DES1 group, the same below).

[0094] Take 0.1mL of 0.25mol / L calcium chloride aqueous solution and 0.5mL of hyaluronidase solution, mix them evenly, and keep them at 37℃ for 20min; add 0.5mL of sample, and keep them at 37℃ for 20min; add 0.5mL of sodium hyaluronate solution, keep them at 37℃ for 30min, and leave them at room temperature for 5min; add 0.1mL of 0.4mol / L NaOH aqueous solution and 0.5mL of acetylacetone solution (dissolve 3.5mL of acetylacetone in 50mL of 1.0mol / L sodium carbonate solution), heat in a boiling water bath for 15min, and then immediately cool on ice for 5min; add 1.0mL of Ehrlich reagent (weigh 0.8g of p-dimethylaminobenzaldehyde and dissolve it in 15mL of concentrated hydrochloric acid and 15mL of anhydrous ethanol), dilute it with 3.0mL of anhydrous ethanol, and leave it for 20min to develop color; and measure the absorbance value at 547nm using a spectrophotometer.

[0095] Hyaluronic acid inhibition rate = [(AB)-(CD)] ÷ (AB) x 100%

[0096] Where: A-absorbance value of control solution;

[0097] B-absorbance value of blank control solution;

[0098] C-absorbance value of sample solution;

[0099] D-absorbance value of sample blank solution.

[0100] The results are shown in Table 4 and Figure 1 As shown, the inhibition effect of the combination of embodiments 2-5 and 6-8 is the best, which is significantly better than the comparative examples 1-3 of the replacement component raw materials, and the independent crocodile oil embodiment 1 and comparative examples 4-5, showing that in the embodiment of the present invention, crocodile oil has a significant synergistic effect after being compounded with tea polyphenols, Centella asiatica, cetearyl alcohol, and amino acids. Wherein, the effect of using a deep eutectic solvent to extract crocodile and then compounding the composition is better than the crocodile oil combination effect (embodiments 7 and 8) prepared by the traditional method, indicating that the effective ingredient content can be further improved after crocodile oil is extracted using a deep eutectic solvent. The hyaluronic acid inhibition rate of a single crocodile oil in Example 1 is higher than the hyaluronic acid inhibition rate of a single crocodile oil extracted by dry boiling extraction method and supercritical fluid extraction method in comparative examples 4 and 5, and it is further confirmed that the use of a deep eutectic solvent to extract crocodile oil is more advantageous than traditional extraction methods; the hyaluronic acid inhibition rate effect of Example 2 is the best.

[0101] Table 4 Hyaluronic acid inhibition rate in different groups

[0102] Serial number Hyaluronic acid inhibition rate / % Example 1 55.3 Example 2 93.6 Example 3 90.2 Example 4 89.1 Example 6 92.3 Example 7 91.4 Example 8 82.3 Comparative Example 1 70.1 Comparative Example 2 76.6 Comparative Example 3 72.8 Comparative Example 4 33.3 Comparative Example 5 41.4

[0103] Test Example 2 Repair Efficacy Test—Hydroxy Radical Scavenging Experiment

[0104] The samples prepared in Examples 1-4, 6-8 and Comparative Examples 1-4 were respectively dissolved and diluted with ultrapure water to 1% as sample solutions. 9 mmol / L FeSO 4 , 9mmol / L ethanol-salicylic acid, then add an appropriate amount of deionized water, and finally add 8.8mmol / L H 2 O 2 Then shake well, heat in a 37℃ water bath for 15 minutes, take out, and measure its absorbance A 0 . Determination of A 0 According to the above method, the sample dilution solutions of Examples 1-4, 6-8 and Comparative Examples 1-4 were added to measure the absorbance A x , A x0When Ax and Ax0 are measured, the reference solution is deionized water. The calculation formula for the scavenging rate of hydroxyl free radicals is:

[0105] -OH removal rate (%) = A 0 -(A x -A x0 ) / A 0 ×100%

[0106] Where: A 0 is the absorbance value of the blank control;

[0107] A x is the absorbance value of the added sample;

[0108] A x0 Without adding color developing agent H 2 O 2 The absorbance of the solution.

[0109] The results are shown in Table 5 and Figure 2 As shown, the -OH scavenging effect of the combination of Examples 2-4 and 6-8 is better than that of Comparative Examples 1-5 and Example 1, and the -OH scavenging effect of the composition of crocodile oil extracted by low eutectic solution in Example 2 and compounded with tea polyphenols, Centella asiatica, cetearyl alcohol and amino acids is the best; the -OH scavenging effect of the crocodile oil extracted by DES in Example 1 is not as good as the compounded composition of Examples 2-4 and 6-8; and the -OH scavenging effect of the crocodile oil extracted by traditional dry boiling extraction method and supercritical fluid extraction method in Comparative Examples 4-5 is worse, among which the -OH scavenging rate of the crocodile oil extracted by dry boiling extraction method is only half of the OH scavenging rate of the crocodile oil extracted by low eutectic solution.

[0110] Table 5 -OH removal rate of different samples / %

[0111]

[0112]

[0113] Test Example 3 Irritation Test—Red Blood Cell Hemolysis Test

[0114] In a 2mL centrifuge tube, add 500μL of the test substance (Examples 1-4, 6-8; Comparative Examples 1-5) and 500μL of 2% sheep erythrocyte suspension (mixed in equal volumes) respectively, and mix thoroughly. Set up 3 parallels for each concentration. Use the solvent (normal saline or PBS) as the negative control (i.e., completely non-hemolytic), and use 0.1% deionized water solution as the positive control (i.e., completely hemolytic). After incubating in a 37°C constant temperature incubator for 3h, centrifuge at 2000rpm / min for 3min, aspirate 10μL of the supernatant, add it to the ELISA plate (add 90μL of normal saline or PBS to each well in advance), mix well, and measure the absorbance (A) at 410nm, 540nm, and 575nm on the ELISA instrument. 410nm , A 540nm , A 575nm ). The calculation formula of hemolysis rate and protein denaturation index is:

[0115] Hemolysis rate (%) = (AC) / (BC) x 100%

[0116] Where A is the average absorbance of each sample concentration at 410 nm;

[0117] B is the average absorbance of the positive control at 410 nm;

[0118] C is the average absorbance of the negative control at 410 nm;

[0119] Protein denaturation index (DI%) = (test substance A 575nm / A 540nm ) / (positive control A 575nm / A 540nm )x100%

[0120] The IP value of the sample is then calculated based on the HC50 value (the concentration of the test substance that causes 50% hemolysis of red blood cells is HC50 (mg / L)) and the DI value: IP = HC50 / DI. The irritation degree of cosmetics is graded according to the RBC experimental classification standard of the European Center for Validation of Alternative Methods (ECVAM), and the specific classification is shown in Table 6.

[0121] Table 6 Irritation classification standard of red blood cell hemolysis test

[0122] IP value Grading ﹥100 Non-irritating 10﹤L / D≤100 Slightly irritating 1﹤L / D≤10 Mild irritant 0.1﹤L / D≤1 Moderate irritation L / D≤0.1 Severe irritation

[0123] The measurement results are shown in Table 7, which shows that the combined IP values ​​of Examples 2-4 and 6-8 in which crocodile oil is compounded with tea polyphenols, Centella asiatica, cetearyl alcohol, and amino acids are all greater than 100, and the single crocodile oil also exhibits non-irritation; the IP values ​​of crocodile oil extracted by traditional dry boiling extraction method and supercritical fluid extraction method are both lower than the IP value of crocodile oil extracted by low eutectic solution, indicating that the composition obtained by compounding crocodile oil extracted by low eutectic solution with other raw materials has an advantage in irritation to the skin; the synergistic effect of carrot seed essential oil compounded with tea polyphenols, Centella asiatica, cetearyl alcohol, and amino acids in Comparative Example 1 is weak, the rosemary extract in the raw material in Comparative Example 2 is irritating to the skin, and 1,3-propylene glycol and ceramide in the raw material in Comparative Example 3 are both irritating to the skin, showing slight irritation to the skin.

[0124] Table 7 IP values ​​of different samples

[0125] Serial number IP value Example 1 134.4 Example 2 181.2 Example 3 163.5 Example 4 161.2 Example 6 170.9 Example 7 171.2 Example 8 146.8 Comparative Example 1 90.5 Comparative Example 2 75.6 Comparative Example 3 56.3 Comparative Example 4 110.8 Comparative Example 5 118.6

[0126] Test Example 4: Anti-allergic repair test - Skin TEWL value and water content test

[0127] The tester's arm was repeatedly torn with 3M tape to perform keratin exfoliation treatment to simulate skin with damaged barrier. The test group was selected as a group in which the anti-allergic repair essential oil combination of Examples 1-4, 6-8 and Comparative Examples 1-5 and crocodile oil were applied after tearing, and the blank control was a group in which the anti-allergic repair essential oil combination was not applied after tearing. The blank control was a group in which no repair product was used, and the skin barrier was repaired slowly by relying on the skin's own repair ability. The water loss (TEWL value) of the test area and the blank area was measured using a skin water loss tester Tewameter TM300, and the skin moisture content was measured using a skin moisture content tester Comeometer 825Coyrage.

[0128] The results are shown in Table 8 and Figure 3 , Figure 4 As shown, compared with the single crocodile oil (Example 1, Comparative Examples 4-5), the TEWL value of the compositions of Examples 2-4 and 6-8 is significantly reduced, and the skin moisture content is also significantly increased, indicating that the damaged skin barrier has begun to repair; the effect is better than that of Comparative Examples 1-3. It shows that the anti-allergic repair essence oil prepared by the present invention can accelerate the repair of the damaged stratum corneum.

[0129] Table 8 Results of skin TEWL values ​​and water content determination of different samples

[0130] Serial number TEWL value Water content / % Example 1 1350 25 Example 2 1200 34 Example 3 1250 28 Example 4 1280 27 Example 6 1240 29 Example 7 1230 30 Example 8 1290 27 Comparative Example 1 1680 14 Comparative Example 2 1650 20 Comparative Example 3 1650 19 Comparative Example 4 1750 13 Comparative Example 5 1870 11

[0131] Test Example 5 Anti-allergic Repair Test—DPPH Free Radical Scavenging Rate Test

[0132] The above anti-allergic essence oil product is subjected to a DPPH free radical scavenging rate test and the results are compared. If the free radical scavenging rate of the anti-allergic essence oil test sample is higher than that of the control sample, it can be considered that the anti-allergic essence oil test sample has a certain anti-allergic repair effect. The above anti-allergic essence oil product is reacted with a DPPH reagent, and the absorbance (OD value) is measured at 517nm after the reaction, and the free radical scavenging rate is calculated according to the following formula:

[0133] Free radical scavenging rate = (A 2 +A 3 -A 1 ) / A 2 ×100%

[0134] Among them, A 1 This product has a DPPH reaction system OD value, A 2 is the OD value of the DPPH reaction system of anhydrous ethanol, A 3 It is the OD value of the reaction system without DPPH (substituted by anhydrous ethanol) of this product.

[0135] The results are shown in Table 9, which show that the free radical scavenging rates of the compositions of Examples 2-4 and 6-8 are higher than those of the single crocodile oil (Example 1, Comparative Examples 4-5), and the effects are better than those of Comparative Examples 1-3. This indicates that the use of the anti-allergic repairing essential oil of the present invention can accelerate the repair of damaged stratum corneum.

[0136] Table 9 DPPH free radical scavenging rate of different samples

[0137]

[0138]

[0139] In summary, after the present invention compounds crocodile oil with other four components, it has a good anti-allergic repair effect, can accelerate the repair of damaged stratum corneum and increase skin moisture content, thereby improving the barrier function of the skin; it has the function of activating cell regeneration, can decompose skin aging damaged cells, accelerate metabolism, and promote skin cells to accelerate the alternation of old and new to achieve the effect of anti-allergic repair. And there is a significant synergistic effect between the components of the composition, and its effect is significantly better than that of each component used alone or any combination of several components, and has a good anti-allergic repair effect. Further, by mixing a low eutectic solvent with crocodile fat for ultrasonic treatment to obtain a crocodile oil with a high extraction rate, it is used to prepare an anti-allergic repair essence oil, and the low eutectic solvent optimized ultrasound-assisted extraction method used not only improves the crocodile oil extraction rate, but also is green and environmentally friendly and can improve the scavenging rate of free radicals, achieving a better antioxidant repair effect.

[0140] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. An anti-allergic repair composition, characterized in that: The invention comprises components in the following mass ratio: crocodile oil: tea polyphenols: centella asiatica: cetearyl alcohol: amino acid = (10-40): (2-6): (3-9): (4-8): (5-15).

2. The composition according to claim 1, characterized in that The invention comprises components in the following mass ratio: crocodile oil: tea polyphenols: Centella asiatica: cetearyl alcohol: amino acid = (15-30): (3-5): (4-8): (5-7): (10-15).

3. The composition according to claim 1 or 2, characterized in that The crocodile oil is obtained by extraction with a low eutectic solvent, and the specific method is: crocodile fat and the low eutectic solvent are mixed, ultrasonic extraction is performed, and the crocodile oil is separated after centrifugation.

4. The composition according to claim 3, characterized in that The low eutectic solvent is prepared from hydrogen bond acceptor benzalkonium chloride and hydrogen bond donor lauryl alcohol; the molar ratio of benzalkonium chloride to lauryl alcohol is (1-3):

1.

5. The composition according to claim 3, characterized in that The ultrasonic extraction conditions are: temperature 30-80°C, ultrasonic power 80-100W, and extraction time 20-90min.

6. Use of the composition according to any one of claims 1 to 5 in repairing sensitive skin or skin barrier.

7. Use of the composition according to any one of claims 1 to 5 in the preparation of anti-allergic repair products.

8. An anti-allergic repair product, characterized in that: A composition comprising any one of claims 1 to 5.

9. The product according to claim 8, characterized in that: The product described is an anti-allergic repair essential oil.

10. The method for preparing the product according to claim 9, characterized in that: Preheat crocodile oil and add water and tea polyphenols, stir, heat up to 85-90℃, and homogenize; then add amino acids, homogenize, cool to 65-70℃, add Centella asiatica and cetearyl alcohol, homogenize, cool in vacuum, and discharge to obtain anti-allergic repair essential oil.

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