Biological protease fermentation device

Abstract

The invention relates to the field of coal-bed gas well drainage and mining, in particular to a biological protease fermentation device which comprises a rotatable fermentation tank, and a mixing module capable of evenly stirring fermentation materials is fixedly arranged in the middle of the interior of the fermentation tank. A tearing module matched with the mixing module to scatter internal deposits is fixedly arranged in the fermentation tank, and a plurality of crushing modules capable of crushing large fermented materials are fixedly arranged on the inner wall of the fermentation tank. Through cooperation with oxygen transmission, growth of microorganisms and synthesis of enzymes are promoted, dead corners in the fermentation tank are reduced, the problem of poor local fermentation is avoided, internal deposits can be scattered, the fermentation materials are prevented from being accumulated at the bottom or on the wall of the tank, uniform distribution of the fermentation materials is ensured, and the fermentation efficiency is improved. Meanwhile, large-sized fermented materials can be effectively filtered and crushed, large particles are prevented from blocking or influencing the fermentation efficiency, and all the fermented materials can be ensured to be in full contact with a culture medium.

Description

Technical Field

[0001] The invention relates to the field of coalbed methane well drainage, in particular to a biological protease fermentation device. Background Art

[0002] Bio-protease fermentation technology is widely used in many industries such as biopharmaceuticals, food processing, and chemicals. As an important biocatalyst, protease plays an important role in many biochemical reactions. In the field of biopharmaceuticals and enzyme engineering, the production of protein enzymes usually relies on fermentation technology. During the fermentation process, microorganisms grow and synthesize the required protein enzymes under specific conditions. In order to improve the yield and quality of protein enzymes, the fermentation process requires strict control of various parameters such as temperature, pH value, dissolved oxygen concentration, etc. In addition, it is also necessary to ensure the uniform distribution of the fermentation product in the fermenter to avoid problems such as local accumulation and blockage in order to improve the fermentation efficiency.

[0003] A Chinese patent with application number 202111291289.3 discloses a biological enzyme fermentation device, including a casing, a fermentation chamber is provided in the casing, a rotating chamber is provided in the lower end wall of the fermentation chamber, a pulley chamber is provided in the rear end wall of the rotating chamber, a meshing chamber connected to the rotating chamber is provided in the upper end wall of the pulley chamber, a power chamber is provided in the rear end wall of the pulley chamber, two liquid storage chambers are symmetrically provided in the left and right end walls of the fermentation chamber, a transmission chamber is provided in the rear end wall of the driven chamber, a base is fixedly connected to the lower end surface of the casing, a stirring mechanism, a liquid changing mechanism and a transmission mechanism are provided in the casing, and a stirring block provided in the stirring mechanism that can reciprocate up and down while rotating and stirring is used to make the stirring more uniform and ensure the stirring effect. Automatic liquid change is achieved through the liquid changing mechanism, which ensures the sealing of the equipment during the liquid change process and avoids the pollution of the equipment caused by manual loading and discharging.

[0004] A Chinese patent with application number 202410954011.7 discloses a biological protease fermentation device, including a fermentation tank body, a tank top shell is arranged on the top of the fermentation tank body, and an upper shell is arranged on the top of the tank top shell. The present invention uses shaking to quickly shake off the raw materials attached to the rotating rod and the stirring rod. After the rotating rod and the stirring rod are used to stir, they are removed from the raw materials, and the flow rate of the raw materials attached to the outside of the rotating rod and the stirring rod is accelerated by shaking, so that the raw materials attached to the rotating rod and the stirring rod can be mixed with the raw materials in the fermentation tank body for fermentation. After the fermentation of the raw materials is completed, the raw materials can be directly discharged. Since the rotating rod and the stirring rod are removed from the raw materials after the stirring of the raw materials is completed, and the raw materials attached to the outside of the rotating rod and the stirring rod are shaken off, when the raw materials are discharged, there is no need to wait for the raw materials attached to the outside of the rotating rod and the stirring rod to flow down, thereby reducing the waiting time.

[0005] However, there are still the following problems in practical applications, specifically: 1. During the fermentation process, due to insufficient stirring, the fermented product may form dead corners in certain areas of the fermentation tank, resulting in uneven distribution of the fermented product. This will not only affect the growth of microorganisms, but also reduce the efficiency of enzyme synthesis.

[0006] 2. During the fermentation process, the fermented material may accumulate at the bottom of the fermentation tank or on the tank wall to form clumps, which will hinder the growth of microorganisms and the synthesis of enzymes. In addition, the accumulated fermented material may also cause uneven pressure inside the fermentation tank, affecting the fermentation effect.

[0007] 3. For large fermentation products or agglomerated fermentation products, traditional fermentation equipment often lacks effective means of breaking them up, resulting in these large fermentation products not being able to fully contact the culture medium, affecting the fermentation efficiency.

[0008] Therefore, how to overcome the above-mentioned technical problems and defects becomes a key issue that needs to be solved. Summary of the invention

[0009] The object of the present invention is to overcome the defects described in the background technology, thereby realizing a biological protease fermentation device, in which the separator can ensure that the fermentation product is evenly stirred in the tank through the fermentation tank body and in cooperation with the internal module, and promote the growth of microorganisms and enzyme synthesis through uniform stirring and efficient oxygen transfer, reduce dead corners in the fermentation tank, avoid the problem of local poor fermentation, and break up internal deposits to avoid the accumulation of fermentation products at the bottom or wall of the tank, thereby ensuring the uniform distribution of the fermentation products. At the same time, it can effectively filter and crush large fermentation products to prevent large particles from clogging or affecting the fermentation efficiency, thereby ensuring that all fermentation products can fully contact the culture medium.

[0010] To achieve the above-mentioned purpose, the technical solution of the present invention is: a biological protease fermentation device, comprising a rotatable fermentation tank, wherein a mixing module capable of uniformly stirring the fermentation product is fixedly arranged in the middle of the fermentation tank. A tearing module that cooperates with the mixing module to break up the internal accumulation is fixedly arranged in the fermentation tank. A plurality of crushing modules capable of crushing large fermentation products are fixedly arranged on the inner wall of the fermentation tank.

[0011] The mixing module comprises a central axis fixedly arranged horizontally in the middle of the fermenter, a plurality of stirring units arranged in a circumferential array on the central axis, and an air supply unit for supplying oxygen to the interior of the central axis.

[0012] In the above-mentioned bio-protease fermentation device, the fermentation tank is a polygonal tank body assembled from a plurality of circular arrays of partitions, and the fermentation tank is horizontally rotatably arranged on a bracket arranged at the bottom thereof.

[0013] A first motor with a reducer is fixedly arranged on the bracket, the output end of the reducer is fixedly arranged at the middle position of the fermentation tank, and a rotating wheel capable of driving the fermentation tank to rotate is also fixedly arranged on the side of the reducer at the opposite end of the fermentation tank.

[0014] A conduit plate is fixedly arranged on the bracket directly below the fermentation tank. The conduit plate is a top-opening structure and a water outlet end thereof is arranged to be tilted downward.

[0015] In the above-mentioned bio-protease fermentation device, the partition is a double-layer jacket design, the inner layer is 316L stainless steel, and the outer layer is carbon steel.

[0016] A heating cavity and a heat dissipation cavity are provided inside the partition. A heating plate is fixedly provided inside the heating cavity, and a plate heat exchanger is fixedly provided inside the heat dissipation cavity.

[0017] A temperature sensor is fixedly arranged inside the fermentation tank.

[0018] A sampling port communicating with the interior is provided on the side of the fermentation tank, and an end cap is threadedly connected to the sampling port.

[0019] In the above-mentioned biological protease fermentation device, the stirring unit includes a plurality of stirring main frames arranged in a circumferential array and fixedly arranged on the central axis, and at least one stirring sub-frame is rotatably arranged on the stirring main frame. A stirring plate is fixedly arranged on the stirring sub-frame, and a mixing groove is opened in the middle of the stirring plate and penetrates along the thickness direction thereof.

[0020] In the above-mentioned bio-protease fermentation device, the air supply unit includes a ventilation cavity coaxially opened inside the central axis, and a plurality of ventilation grooves are opened in a circular array outside the central axis, and each of the ventilation grooves is connected to the ventilation cavity.

[0021] A microporous aeration tube is fixedly arranged inside the ventilation cavity, an oxygen generator is fixedly arranged at the outer end of the fermenter, and the gas transmission end of the oxygen generator is connected to the end of the microporous aeration tube. A sterile air filter is fixedly arranged on the inner wall of the fermenter, and the sterile air filter is located at the end of the microporous aeration tube between the oxygen generator and the microporous aeration tube.

[0022] In the above-mentioned biological protease fermentation device, the tearing module includes a plurality of slide grooves opened on the circumference of the central axis on the side of the ventilation groove, and the slide grooves are staggered with the corresponding ventilation grooves. A plurality of cutting rods that can slide in the corresponding slide grooves are arranged in a circumferential array on the central axis, and a lead screw is arranged inside the slide groove to rotate along its length direction, and the lead screw passes through the end of the cutting rod and is threaded with the cutting rod. A multi-axis controller that can drive the corresponding lead screws to rotate is fixedly arranged at the outer end of the fermentation tank, and a second motor is fixedly arranged on the side of the multi-axis controller.

[0023] In the above-mentioned bio-protease fermentation device, the crushing module includes a cutting unit. The cutting unit includes a cutting box fixedly arranged on each of the partitions, and a plurality of partition columns are fixedly arranged at one end of the interior of the cutting box, and a filter tank is formed between the partition columns. Two cutting rollers in opposite positions are rotatably arranged inside the cutting box at the front end of the filter tank, and a spiral blade is fixedly arranged on the cutting stick. A lever is rotatably arranged inside the cutting box at the rear end of the partition column, and a plurality of cleaning plates are arranged on the lever, and each group of cleaning plates can penetrate into the corresponding filter tank.

[0024] The cleaning plate comprises a plurality of shifting plates arranged in a circumferential array on a shifting rod, and a squeezing knife which can be inserted into the filter tank is fixedly arranged at the end of the shifting plate.

[0025] In the above-mentioned bio-protease fermentation device, the crushing module also includes a power unit, which includes a sealing box fixedly arranged on the side of the cutting box, and an electric rotating table for driving the lever to rotate is fixedly arranged inside the sealing box. A first pulley is fixedly arranged on the side of the electric rotating table, and the end of the cutting roller penetrates the side wall of the cutting box and extends into the sealing box, and a gear is fixedly arranged on the end of each roller, and the two gears are meshed with each other. A second pulley is fixedly arranged on the corresponding gear, and a power belt is sleeved on the first pulley and the second pulley.

[0026] Compared with the prior art, the bioprotease fermentation device of the present invention has at least the following beneficial effects: 1. The bio-protease fermentation device of the present invention can ensure that the fermented material is evenly stirred in the tank through the rotation of the fermentation tank body and the multiple stirring units on the central axis. The uniform stirring and efficient oxygen transfer can promote the growth of microorganisms and the synthesis of enzymes. At the same time, the uniform stirring reduces the dead corners in the fermentation tank and avoids the problem of poor local fermentation. The ventilation cavity and microporous aeration tube inside the central axis can evenly distribute oxygen in the fermentation liquid, improve the oxygen transfer efficiency, and cooperate with the stirring to improve the quality and consistency of the final product.

[0027] 2. The bio-protease fermentation device of the present invention breaks up the internal deposits by sliding the cutting rod in the slide groove, avoiding the accumulation of fermented products on the bottom or wall of the tank, thereby ensuring the uniform distribution of the fermented products. At the same time, the cutting rollers in the cutting box and the cleaning plates on the lever can effectively filter and break up large fermented products, preventing large particles from clogging or affecting the fermentation efficiency, thereby ensuring that all fermented products can fully contact the culture medium.

[0028] 3. The bio-protease fermentation device of the present invention has a double-layer jacket design of the partition, with the inner layer being 316L stainless steel and the outer layer being carbon steel, which enhances the durability and thermal insulation performance of the tank body, and can also monitor the internal temperature of the fermentation tank in real time through a temperature sensor, and accurately control it through a heating plate and a plate heat exchanger to ensure that the fermentation process is carried out under optimal temperature conditions, thereby improving the fermentation efficiency, and through precise temperature control, reducing unnecessary energy consumption and improving energy utilization efficiency.

[0029] 4. The bio-protease fermentation device of the present invention realizes automatic control of functions such as stirring, gas supply, tearing, and crushing through automated equipment such as a multi-axis controller and an electric rotating table, thereby improving operating efficiency, reducing human errors, reducing manual operations, and improving production safety.

[0030] 5. The bio-protease fermentation device of the present invention is provided with a sampling port located on the side of the fermentation tank, which is convenient for regular sampling and testing to ensure the controllability of the fermentation process and the quality of the product. At the same time, the collection and discharge of the fermentation liquid is facilitated by the manifold disposed under the fermentation tank, thereby improving the operating environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the internal structure of the present invention; Figure 3 It is a schematic diagram of the position of the heat dissipation cavity of the present invention; Figure 4 It is a schematic diagram of the structure of the crushing module of the present invention; Figure 5 The present invention Figure 4 A magnified image of area A; Figure 6 It is a schematic diagram of the structure of the stirring unit of the present invention; Figure 7 It is a schematic diagram of the central axis position of the present invention; Figure 8 It is a schematic diagram of the tearing module structure of the present invention.

[0032] In the figure: 1. Fermentation tank; 2. Hybrid module; 21. Middle axis; 22. stirring unit; 221. stirring main frame; 222. stirring sub-frame; 223. stirring plate; 224. mixing tank; 23. Air supply unit; 231. Ventilation cavity; 232. Ventilation slot; 233. Microporous aeration tube; 234. Oxygen generator; 235. Sterile air filter; 3. tearing module; 31. slide slot; 32. cutting rod; 33. lead screw; 34. multi-axis controller; 35. second motor; 4. Crushing module; 41. Cutting unit; 411. Cutting box; 412. Partition column; 413. Filter tank; 414. Cutting roller; 415. Spiral blade; 416. Push rod; 417. Cleaning plate; 418. Push plate; 419. Extrusion knife; 42. Power unit; 421. Sealing box; 422. Electric rotating table; 423. First pulley; 424. Gear; 425. Second pulley; 426. Power belt; 5. Partition; 6. Bracket; 7. Speed ​​reducer; 8. First motor; 9. Rotor; 10. Manifold; 11. Heating chamber; 12. Heat dissipation chamber; 13. Heating plate; 14. Plate heat exchanger; 15. Temperature sensor; 16. Sampling port; 17. End cover. DETAILED DESCRIPTION

[0033] The bioprotease fermentation device of the present invention is described in more detail below with reference to the accompanying drawings and through specific implementation methods.

[0034] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0035] See also Figure 1-Figure 8, a biological protease fermentation device of this embodiment can ensure that the fermentation product is uniformly stirred in the tank, and through uniform stirring and efficient oxygen transfer, promote the growth of microorganisms and enzyme synthesis, reduce dead corners in the fermentation tank 1, avoid the problem of poor local fermentation, and can also break up the internal accumulation, avoid the accumulation of fermentation products on the bottom or wall of the tank, ensure the uniform distribution of the fermentation product, and effectively filter and crush large fermentation products, prevent large particles from clogging or affecting the fermentation efficiency, and ensure that all fermentation products can fully contact the culture medium. In this embodiment, it mainly includes a rotatable fermentation tank 1. The fermentation tank 1 is a polygonal structure tank body assembled by a circular array of multiple partitions 5. The partition 5 is a double-layer jacket design, the inner layer is 316L stainless steel, and the outer layer is carbon steel. Using 316L stainless steel as the inner layer material improves the corrosion resistance of the equipment and prolongs its service life. A heating chamber 11 and a heat dissipation chamber 12 are opened inside the partition 5. A heating plate 13 is fixedly arranged inside the heating chamber 11, and a plate heat exchanger 14 is fixedly arranged inside the heat dissipation chamber 12. A temperature sensor 15 is fixedly arranged inside the fermentation tank 1. The temperature sensor 15 detects the internal temperature in real time, and the heating plate 13 and the plate heat exchanger 14 realize accurate temperature control to ensure that the fermentation process is carried out under the optimal temperature condition. A sampling port 16 connected to the inside is opened on the side of the fermentation tank 1, and an end cap 17 is threadedly connected to the sampling port 16. It is convenient for regular sampling and testing to ensure the controllability of the fermentation process and the quality of the product.

[0036] The fermentation tank 1 is horizontally rotatably arranged on a bracket 6 arranged at the bottom thereof. A first motor 8 with a reducer 7 is fixedly arranged on the bracket 6, and the output end of the reducer 7 is fixedly arranged at the middle position of the fermentation tank 1. A rotating wheel 9 that can drive the fermentation tank 1 to rotate is also fixedly arranged on the side of the reducer 7 at the opposite end of the fermentation tank 1. The automatic rotation of the fermentation tank 1 body is realized to ensure that the fermented material is evenly stirred in the tank, and it can be manually rotated under special circumstances. A manifold 10 is fixedly arranged on the bracket 6 directly below the fermentation tank 1. The manifold 10 is a top-opening structure and its water outlet end is tilted downward. It is convenient to collect and discharge the fermentation liquid and improve the operating environment.

[0037] To achieve internal fermentation agitation, see Figure 2 , Figure 6 and Figure 7In this embodiment, a mixing module 2 for uniformly stirring the fermented product is fixedly arranged at the middle position of the fermenter 1. The mixing module 2 includes a central axis 21 fixedly arranged horizontally at the middle position of the fermenter 1. A plurality of stirring units 22 are arranged in a circumferential array on the central axis 21. The stirring unit 22 includes a plurality of stirring main frames 221 arranged in a circumferential array and fixedly arranged on the central axis 21, and at least one stirring sub-frame 222 is rotatably arranged on the stirring main frame 221. A stirring plate 223 is fixedly arranged on the stirring sub-frame 222, and a mixing groove 224 is provided in the middle of the stirring plate 223 that penetrates along its thickness direction. When the fermenter 1 rotates, the stirring main frame 221 and the stirring sub-frame 222 inside the fermenter 1 rotate synchronously, thereby uniformly stirring the fermented product inside. In this process, the stirring plate 223 is moved by the fermented product and the liquid to increase the stirring and mixing effect. The separation of the accumulated stirring products is achieved by the stirring plate 223 and the mixing groove 224, reducing the dead angle in the fermenter 1 and avoiding the problem of poor local fermentation.

[0038] The interior of the central axis 21 is provided with an air supply unit 23 for supplying oxygen to the interior. The air supply unit 23 includes a ventilation cavity 231 coaxially opened inside the central axis 21, and a plurality of ventilation grooves 232 are opened in a circular array outside the central axis 21, and each of the ventilation grooves 232 is connected to the ventilation cavity 231. A microporous aeration tube 233 is fixedly arranged inside the ventilation cavity 231, and the microporous aeration tube 233 is a mature prior art and will not be described in detail here. An oxygen generator 234 is fixedly arranged at the outer end of the fermentation tank 1, and the oxygen generator 234 is a mature prior art and will not be described in detail here. The gas transmission end of the oxygen generator 234 is connected to the end of the microporous aeration tube 233. A sterile air filter 235 is fixedly arranged on the inner wall of the fermentation tank 1, and the sterile air filter 235 is a mature prior art and will not be described in detail here. The sterile air filter 235 is located at the end of the microporous aeration tube 233 between the oxygen generator 234 and the microporous aeration tube 233. The oxygen generator 234 is controlled to work, and oxygen is filtered through the sterile air filter 235 and then transported to the inside of the microporous aeration tube 233. At this time, the oxygen enters the inside of the fermenter 1 through the ventilation groove 232, thereby supplying oxygen to the inside of the fermenter 1, promoting the growth of microorganisms and the synthesis of enzymes.

[0039] In order to break up the internal accumulation, in this embodiment, see Figure 2 , Figure 6 , Figure 7 and Figure 8The fermentation tank 1 is fixedly provided with a tearing module 3 that cooperates with the mixing module 2 to break up the internal deposits. The tearing module 3 includes a plurality of chute 31 circumferentially provided on the central axis 21 at the side of the ventilation groove 232, and the chute 31 is staggered with the corresponding ventilation groove 232. A plurality of cutting rods 32 that can slide in the corresponding chute 31 are arranged in a circumferential array on the central axis 21, and a lead screw 33 is arranged inside the chute 31 to rotate along its length direction. The lead screw 33 passes through the end of the cutting rod 32 and is threaded with the cutting rod 32. A multi-axis controller 34 that can drive the corresponding lead screw 33 to rotate is fixedly provided at the outer end of the fermentation tank 1. The multi-axis controller 34 is a mature existing technology and will not be described in detail here. A second motor 35 is fixedly provided on the side of the multi-axis controller 34. The second motor 35 is controlled to work, so that the lead screw 33 is driven to rotate through the multi-axis controller 34. At this time, the lead screw 33 drives the cutting rod 32 to slide in the slide groove 31 to break up the internal deposits, prevent the fermented material from accumulating at the bottom or on the wall of the tank, and ensure the uniform distribution of the fermented material.

[0040] For filtering and crushing large fermentations, see Figure 2 , Figure 4 and Figure 5 , a plurality of crushing modules 4 capable of crushing large fermented products are fixedly arranged on the inner wall of the fermentation tank 1. The crushing module 4 includes a cutting unit 41. The cutting unit 41 includes a cutting box 411 fixedly arranged on each of the partitions 5, and a plurality of partition columns 412 are fixedly arranged at one end of the interior of the cutting box 411, and a filter tank 413 is formed between the partition columns 412. Two cutting rollers 414 in opposite positions are rotatably arranged inside the cutting box 411 at the front end of the filter tank 413, and a spiral blade 415 is fixedly arranged on the cutting roller. A lever 416 is rotatably arranged inside the cutting box 411 at the rear end of the partition column 412, and a plurality of cleaning plates 417 are arranged on the lever 416, and each group of cleaning plates 417 can penetrate into the corresponding filter tank 413. The cleaning plate 417 includes a plurality of dial plates 418 arranged in a circumferential array on the dial plate 416, and an extrusion knife 419 that can be inserted into the filter tank 413 is fixedly arranged at the end of the dial plate 418.

[0041] The crushing module 4 also includes a power unit 42, which includes a sealing box 421 fixedly arranged on the side of the cutting box 411, and an electric rotating table 422 for driving the lever 416 to rotate is fixedly arranged inside the sealing box 421. The electric rotating table 422 is a mature existing technology and will not be described in detail here. A first pulley 423 is fixedly arranged on the side of the electric rotating table 422, and the end of the cutting roller 414 penetrates the side wall of the cutting box 411 and extends into the sealing box 421, and a gear 424 is fixedly arranged on its end, and the two gears 424 are meshed with each other. A second pulley 425 is fixedly arranged on the corresponding gear 424, and a power belt 426 is sleeved on the first pulley 423 and the second pulley 425.

[0042] When the fermentation tank 1 rotates, large fermented products will accumulate at the bottom and enter the interior of the cutting box 411. At this time, the partition column 412 will keep the fermented products inside the cutting box 411. Then, the electric rotating table 422 drives the rotating rod to rotate, and the corresponding cutting roller 414 is driven to rotate by the power belt 426 and the gear 424, so that the fermented products are cut by the relative spiral blade 415. The cut fermented products are discharged from the cutting box 411 through the filter tank 413. At this time, the accumulated fermented products are cut and separated again by the rotating paddle 418 and the extrusion knife 419. Prevent large particles from clogging or affecting the fermentation efficiency, and ensure that all fermented products can fully contact the culture medium.

[0043] The method for using the bio-protease fermentation device of the present invention is as follows: first, the first motor 8 is controlled to work, so that the fermentation tank 1 rotates on the bracket 6 to ensure that the fermented product is uniformly stirred in the tank. At the same time, the corresponding partition 5 can be removed and the fermentation tank 1 can be manually rotated when it is necessary to discharge, so as to collect and discharge the fermented liquid. When the fermentation tank 1 rotates, the stirring main frame 221 and the stirring sub-frame 222 inside the fermentation tank 1 rotate synchronously, so as to uniformly stir the fermented product inside. In this process, the stirring plate 223 is moved by the fermentation product and the liquid to increase the stirring and mixing effect. And the separation of the accumulated stirring product is achieved by the stirring plate 223 and the mixing tank 224, so as to reduce the dead angle in the fermentation tank 1 and avoid the problem of poor local fermentation. At the same time, the oxygen generator 234 is controlled to work, and the oxygen is filtered through the sterile air filter 235 and then transported to the inside of the microporous aeration pipe 233. At this time, the oxygen enters the inside of the fermentation tank 1 through the ventilation groove 232, so as to supply oxygen to the inside of the fermentation tank 1, and promote the growth of microorganisms and the synthesis of enzymes. During this process, the internal temperature is detected in real time by the temperature sensor 15, and accurate temperature control is achieved through the heating plate 13 and the plate heat exchanger 14 to ensure that the fermentation process is carried out under the optimal temperature conditions. At the same time, regular sampling and testing are carried out through the sampling port 16 to ensure the controllability of the fermentation process and the quality of the product.

[0044] When the fermentation tank 1 rotates, a second motor 35 is fixedly provided on the side of the multi-axis controller 34. The second motor 35 is controlled to work, so that the multi-axis controller 34 drives the lead screw 33 to rotate. At this time, the lead screw 33 drives the cutting rod 32 to slide in the slide groove 31, so as to break up the internal accumulation, prevent the fermentation from accumulating on the bottom or wall of the tank, and ensure the uniform distribution of the fermentation. And when the fermentation tank 1 rotates, large fermentation will accumulate at the bottom, and then enter the inside of the cutting box 411, and the partition column 412 will leave the fermentation inside the cutting box 411. Then the electric rotating table 422 drives the rotating rod to rotate, and the corresponding cutting roller 414 is driven to rotate by the power belt 426 and the gear 424, so that the fermentation is cut by the relative spiral blade 415. The cut fermentation is discharged from the cutting box 411 through the filter tank 413, and the accumulated fermentation is cut and separated again by the rotating paddle 418 and the extrusion knife 419. Prevent large particles from clogging or affecting fermentation efficiency, and ensure that all fermentation products have sufficient contact with the culture medium.

[0045] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning understood by a person of ordinary skill in the field to which the invention belongs. The use of "one" or "an" and other similar words in the specification and claims of this application does not necessarily indicate a quantitative limitation. "Include" or "comprises" and other similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and other similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

[0046] The exemplary implementation modes of the present invention are described in detail above with reference to the preferred embodiments. However, it can be understood by those skilled in the art that, without departing from the concept of the present invention, various modifications and variations can be made to the above-mentioned specific embodiments, and various combinations of the technical features and structures proposed in the present invention can be made without exceeding the protection scope of the present invention.

Claims

1. A biological protease fermentation device, characterized in that: The invention comprises a rotatable fermentation tank (1), wherein a mixing module (2) capable of uniformly stirring the fermented material is fixedly arranged in the middle of the fermentation tank (1), a tearing module (3) capable of cooperating with the mixing module (2) to break up the accumulated material inside the fermentation tank (1) is fixedly arranged inside the fermentation tank (1), and a plurality of crushing modules (4) capable of crushing large fermented materials are fixedly arranged on the inner wall of the fermentation tank (1); The mixing module (2) comprises a central axis (21) fixedly arranged horizontally in the middle of the fermentation tank (1), a plurality of stirring units (22) are arranged in a circular array on the central axis (21), and an air supply unit (23) capable of supplying oxygen to the interior is arranged inside the central axis (21).

2. A biological protease fermentation device according to claim 1, characterized in that: The fermentation tank (1) is a tank body with a polygonal structure formed by assembling a plurality of partitions (5) in a circular array. The fermentation tank (1) is horizontally rotatably arranged on a bracket (6) arranged at the bottom thereof. A first motor with a reducer is fixedly arranged on the bracket, an output end of the reducer (7) is fixedly arranged at a middle position of the fermentation tank (1), and a rotating wheel (9) capable of driving the fermentation tank (1) to rotate is also fixedly arranged on the side of the reducer (7) at the opposite end of the fermentation tank (1); A baffle (10) is fixedly arranged on the bracket (6) directly below the fermentation tank (1); the baffle (10) is a top-opening structure and its water outlet end is arranged to be tilted downward.

3. A biological protease fermentation device according to claim 2, characterized in that: The partition (5) is a double-layer jacket design, the inner layer is 316L stainless steel, and the outer layer is carbon steel; A heating chamber (11) and a heat dissipation chamber (12) are provided inside the partition (5); a heating plate (13) is fixedly provided inside the heating chamber (11), and a plate heat exchanger (14) is fixedly provided inside the heat dissipation chamber (12); A temperature sensor (15) is fixedly arranged inside the fermentation tank (1); The side of the fermentation tank (1) is provided with a sampling port (16) which is connected to the interior, and an end cap (17) is threadedly connected to the sampling port (16).

4. A biological protease fermentation device according to claim 1, characterized in that: The stirring unit (22) comprises a plurality of stirring main frames (221) which are arranged in a circumferential array and fixedly disposed on the central axis (21); at least one stirring sub-frame (222) is rotatably disposed on the stirring main frame (221); a stirring plate (223) is fixedly disposed on the stirring sub-frame (222); and a mixing groove (224) is provided in the middle of the stirring plate (223) and penetrates along the thickness direction thereof.

5. A biological protease fermentation device according to claim 4, characterized in that: The air supply unit (23) comprises a ventilation cavity (231) coaxially provided inside the central axis (21), and a plurality of ventilation grooves (232) are provided in a circumferential array outside the central axis (21), and each of the ventilation grooves (232) is connected to the ventilation cavity (231); A microporous aeration tube (233) is fixedly arranged inside the ventilation cavity (231); an oxygen generator (234) is fixedly arranged at the outer end of the fermentation tank (1); a gas transmission end of the oxygen generator (234) is connected to the end of the microporous aeration tube (233); a sterile air filter (235) is fixedly arranged on the inner wall of the fermentation tank (1); the sterile air filter (235) is located at the end of the microporous aeration tube (233) between the oxygen generator (234) and the microporous aeration tube (233).

6. A biological protease fermentation device according to claim 5, characterized in that: The tearing module (3) comprises a plurality of slide grooves (31) provided on the circumference of the central axis (21) at the side of the ventilation groove (232), the slide grooves (31) being arranged in an interlaced manner with the corresponding ventilation grooves (232), a plurality of cutting rods (32) which can slide in the corresponding slide grooves (31) are arranged in an array on the circumference of the central axis (21), a lead screw (33) is arranged inside the slide groove (31) so as to rotate along its length direction, the lead screw (33) passes through the end of the cutting rod (32) and is threadedly arranged with the cutting rod (32), a multi-axis controller (34) which can drive the corresponding lead screws (33) to rotate is fixedly arranged at the outer end of the fermentation tank (1), and a second motor (35) is fixedly arranged on the side of the multi-axis controller (34).

7. A biological protease fermentation device according to claim 2, characterized in that: The crushing module (4) comprises a cutting unit (41), and the cutting unit (41) comprises a cutting box (411) fixedly arranged on each of the partitions (5); a plurality of partition columns (412) are fixedly arranged at one end of the interior of the cutting box (411); a filter groove (413) is formed between the partition columns (412); two cutting rollers (414) are rotatably arranged in opposite positions in the interior of the cutting box (411) at the front end of the filter groove (413); a spiral blade (415) is fixedly arranged on the cutting roller; a lever (416) is rotatably arranged in the interior of the cutting box (411) at the rear end of the partition column (412); a plurality of cleaning plates (417) are arranged on the lever (416); each group of cleaning plates (417) can penetrate into the interior of the corresponding filter groove (413); The cleaning plate (417) comprises a plurality of shifting plates (418) arranged in a circumferential array on a shifting rod (416), and a squeezing knife (419) which can be inserted into the filter tank (413) is fixedly arranged at the end of the shifting plate (418).

8. A biological protease fermentation device according to claim 7, characterized in that: The crushing module (4) also includes a power unit (42), the power unit (42) includes a sealing box (421) fixedly arranged on the side of the cutting box (411), an electric rotating table (422) for driving the lever (416) to rotate is fixedly arranged inside the sealing box (421), a first pulley (423) is fixedly arranged on the side of the electric rotating table (422), the end of the cutting roller (414) passes through the side wall of the cutting box (411) and extends into the sealing box (421), and a gear (424) is fixedly arranged on the end of each roller, the two gears (424) are meshed with each other, and a second pulley (425) is fixedly arranged on the corresponding gear (424), and a power belt (426) is sleeved on the first pulley (423) and the second pulley (425).

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