Ultrasonic response nanometer heterojunction material and preparation method and application thereof

Abstract

The invention discloses a nano heterojunction material with ultrasonic response sterilization aiming at pain points of clinical bacterial infection. The nano heterojunction material can promote osteogenic differentiation of stem cells and vascularization of endothelial cells. The nano heterojunction is composed of CuFe2O4 nano particles and MoS2 quantum dots, and is prepared by a hydrothermal one-pot method. The nano heterojunction prepared by the method has good cell compatibility, and can respond to ultrasonic stimulation to generate hydroxyl radicals, singlet oxygen and other active oxygen components to kill surrounding bacteria. Besides, copper and iron ions released by the nano heterojunction can promote the expression of osteogenesis related genes of stem cells and promote the expression of angiogenesis related genes of endothelial cells, so that the effect of accelerating bone repair is achieved. The method can be widely applied to the fields related to bone infection treatment or other bacterial infection or bacterial pollution.

Description

Technical Field

[0001] The present invention relates to the technical field of medical nano-preparations, and in particular to a heterojunction nano-material and a preparation method and application thereof. Background Art

[0002] According to the World Health Organization (WHO), millions of people die from bacterial infections every year worldwide. Among them, deaths caused by hospital infections account for a considerable proportion. According to an analysis of hospital infection data in the United States, more than 75,000 patients die from hospital infections each year, and this number may be higher in developing countries. Antibiotics are the main means of clinical treatment and prevention of infection. However, with the widespread use and improper use of antibiotics, bacterial resistance continues to increase, resulting in the failure of previously effective antibiotics to certain bacteria, limiting treatment options and increasing the difficulty and risk of treatment.

[0003] Nanotechnology has unique advantages, including large specific surface area, special physical and chemical properties, etc., and can be used to prepare nanomaterials with antibacterial activity. However, the antibacterial nanoadjuvants currently developed mainly contain antibacterial metal elements such as zinc, silver, and copper, or nanoadjuvants that respond to near-infrared light. Although the former can kill bacteria efficiently, it is concentration-dependent, and once the concentration is too high, it is easy to cause damage to normal tissue cells. The latter is limited by the penetration depth of near-infrared light, and it is difficult to remove bacteria in deep tissues (such as bones), and near-infrared light with too high power can also burn tissues. Therefore, ultrasound-responsive materials with good penetration have become a research hotspot in recent years. In particular, for bone infections, it is of great significance to develop nanomaterials that have efficient ultrasound response to produce reactive oxygen species, and at the same time can promote bone tissue repair without ultrasound. Moreover, the ability to prepare nanomaterials with efficient ultrasound response to produce reactive oxygen species at low cost will also have a far-reaching impact on the wide application in medicine. Summary of the invention

[0004] The purpose of the present invention is to solve the dual needs of antibacterial and bone repair in the treatment of bone infection, and to propose a heterojunction nanomaterial with high-efficiency ultrasonic response and a preparation method and application thereof.

[0005] To achieve the above object, a method for preparing a nano heterojunction adjuvant composed of copper iron oxide and molybdenum disulfide comprises the following steps: Step 1: Add copper salt and iron salt to ethylene glycol respectively and dissolve them by ultrasonication. The copper salt is at least one of copper chloride, copper nitrate and copper sulfate, and the iron salt is at least one of iron chloride, iron nitrate and iron sulfate; Step 2: add sodium acetate to the mixed solution obtained in step 1 and stir evenly. In the presence of sodium acetate and oxygen, Cu 2++Fe 3+ +O2→CuFe2O4; Step 3, adding molybdenum disulfide quantum dots to the mixed solution obtained in step 2, and stirring evenly; Step 4, pouring the mixed solution obtained in step 3 into a polytetrafluoroethylene reactor, and placing a stainless steel outer lining, and reacting in an oven for a period of time, the molybdenum disulfide quantum dots are negatively charged, the CuFe2O4 is positively charged, and the molybdenum disulfide quantum dots are electrostatically adsorbed to the surface of CuFe2O4 to form a nano heterojunction of MoS2 and CuFe2O4; Step 5, centrifuging the reaction mixture in a centrifuge, adding deionized water, and dispersing by ultrasonication, and then centrifuging again in a centrifuge to remove the supernatant; Step six, placing the product obtained in step five into a vacuum drying oven for drying.

[0006] Preferably, the copper salt used is copper chloride, the iron salt used is ferric chloride, and the concentration of copper chloride is 4.25 mg / mL, and the concentration of ferric chloride is 13.5 mg / mL.

[0007] Preferably, the sodium acetate added in step 2 is 25 mg / mL, and the molybdenum disulfide quantum dots added in step 3 is 3.75 mg / mL.

[0008] Preferably, the reaction temperature of step 4 is 180° C. and the reaction time is 24 hours.

[0009] Preferably, in step five, the mixed solution is placed in a 10,000 g centrifuge for 10 minutes, the supernatant is removed, and then deionized water is added and ultrasonically dispersed, and then centrifuged in a 10,000 g centrifuge for 10 minutes, and the supernatant is removed.

[0010] An application of a heterojunction nanomaterial, and the application of the nano heterojunction in bone infection or other bacterial infection or bacterial contamination.

[0011] Some reactions of the local surface of nano-heterojunction CuFe2O4·0.12MoS2 under ultrasonic stimulation: Under the action of ultrasound, ultrasonic electrons are replaced by e and holes are replaced by h.

[0012] CuFe2O4•0.12MoS2+4H2O→Cu 2+ +Fe 3+ +8OH-+0.12 MoS2 h+H2O→·OH+H + O2 1 O2 e+Cu 2+ →Cu+ The nano heterojunction generates active oxygen under ultrasound and releases copper ions and iron ions in a normal environment. Ultrasound causes electron-hole pairs to be generated at the heterojunction interface, thereby generating the following reaction to generate active oxygen and monovalent copper ions.

[0013] Through active oxygen (including OH, 1 O2) causes oxidative stress damage to bacteria, and then cooperates with Cu + Inducing copper death of bacteria, thereby killing Staphylococcus aureus; copper ions and iron ions are biologically active ions and are also present in large quantities in bones. Providing these two ions can directly promote the bone repair process.

[0014] Compared with the prior art, the beneficial effects of the present invention are: The present invention proposes to construct a nano heterojunction adjuvant of copper iron oxide and molybdenum disulfide. The iron, oxygen, copper, sulfur, molybdenum, etc. in the nano adjuvant all have good biocompatibility. The interface of the nano heterojunction is conducive to the adsorption of oxygen, which is conducive to the conduction between electrons and oxygen, so that under ultrasonic stimulation, the separation of interfacial electron holes can be accelerated, and active oxygen components such as hydroxyl radicals and singlet oxygen can be further generated. In addition, the electrons generated under ultrasonic stimulation can reduce divalent copper ions and produce monovalent copper ions, thereby inducing copper death of bacteria. Therefore, the active oxygen generated by the nano heterojunction adjuvant under ultrasonic stimulation can coordinate copper death and efficiently kill bacteria. On the other hand, the copper ions and iron ions released by the nano heterojunction can regulate the behavior of stem cells and endothelial cells and accelerate bone repair.

[0015] The nanoformulation involved in the present invention is completed by a hydrothermal one-step method, and its process is simple and conducive to large-scale production. Secondly, the raw materials used include copper salts, iron salts, sodium acetate, etc., which are all conventional safety reagents, convenient to purchase, and low in price, and molybdenum disulfide quantum dots can be purchased directly, and the amount used is relatively small, so the preparation is safe and economical. Finally, the nano heterojunction of copper iron oxide and molybdenum disulfide prepared by the present invention can respond to ultrasonic stimulation, produce reactive oxygen and monovalent copper ions, kill bacteria by oxidative stress damage and copper death, and can also promote osteogenic differentiation of bone stem cells and vascularization of endothelial cells through the released copper ions and iron ions, accelerate bone repair, and is suitable for clinical bacterial infections, especially the treatment of bone-related infections. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0017] Figure 1Scanning electron microscopy (SEM) (a), transmission electron microscopy (TEM) (b), high-resolution TEM (c), and element distribution (d) of the nano-heterojunction material (CFO@MoS2) of the present invention.

[0018] Figure 2 The X-ray diffraction patterns (XRD) of copper iron oxide (CFO) and CFO@MoS2 of the present invention.

[0019] Figure 3 The ability of four groups of samples (control group, CFO, MoS2, CFO@MoS2) to catalyze dichlorodihydrofluorescein-acetoacetate (DCFH-DA) to produce green fluorescence under ultrasonic stimulation (a), and the ability of CFO@MoS2 samples to produce hydroxyl radicals (b) and singlet oxygen (c) with and without ultrasonic stimulation was detected by electron spin resonance (ESR).

[0020] Figure 4 SEM images of four groups of samples (control group, CFO, MoS2, CFO@MoS2) after co-culture with bacteria with or without ultrasonic stimulation.

[0021] Figure 5 Bacterial live or dead staining images of four groups of samples (control group, CFO, MoS2, CFO@MoS2) after co-culture with bacteria with or without ultrasonic stimulation.

[0022] Figure 6 Figure 2 shows the cell death and viability staining images of four groups of samples (control group, CFO, MoS2, CFO@MoS2) after co-culture with mouse embryonic mesenchymal stem cells (C3H10T1 / 2) (a), and the cell activity detected by CCK-8 (b).

[0023] Figure 7 The expression of osteogenesis-related genes in four groups of samples (control group, CFO, MoS2, CFO@MoS2) after co-culture with C3H10T1 / 2 for 3 and 7 days, respectively.

[0024] Figure 8 The expression of angiogenesis-related genes in human umbilical vein endothelial cells (HUVECs) of the present invention after being cultured with four groups of samples (control group, CFO, MoS2, CFO@MoS2) for 3 and 7 days respectively. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be understood that these descriptions are only exemplary and are not intended to limit the scope of the present invention. In addition, in the following description, the description of well-known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0026] Example 1 170 mg of copper chloride and 540 mg of ferric chloride were added to 40 mL of ethylene glycol and ultrasonically dissolved for 2 hours. Then 1 g of sodium acetate was added and mixed for 30 minutes. The above solution was then poured into a 100 mL polytetrafluoroethylene reactor, and a stainless steel liner was placed in it, and it was reacted in an electric heated blast drying oven at 180°C for 24 hours. The obtained nanoadjuvant was centrifuged in a 10,000g centrifuge for 10 minutes, the supernatant was removed, and then deionized water was added and ultrasonically dispersed. Then it was centrifuged in a 10,000g centrifuge for 10 minutes, the supernatant was removed, and it was dried in a vacuum drying oven. The obtained sample was labeled CFO.

[0027] like Figure 2 As shown, Figure 2 The XRD diagram of the nano adjuvant obtained by the modification treatment of this embodiment is shown in FIG. As can be seen from the figure, there are characteristic peaks representing copper iron oxide.

[0028] Example 2 170 mg of copper chloride and 540 mg of ferric chloride were added to 40 mL of ethylene glycol and ultrasonically dissolved for 2 hours. Then 1 g of sodium acetate was added and mixed for 30 minutes. Then 150 mg of molybdenum disulfide quantum dots were added and mixed for 1 hour. The above solution was then poured into a 100 mL polytetrafluoroethylene reactor, and a stainless steel liner was placed in it, and it was reacted in an electric blast drying oven at 180°C for 24 hours. The obtained nanoadjuvant was centrifuged in a 10000g centrifuge for 10 minutes, the supernatant was removed, and then deionized water was added and ultrasonically dispersed. Then it was centrifuged in a 10000g centrifuge for 10 minutes, the supernatant was removed, and it was dried in a vacuum drying oven. The obtained sample was labeled CFO@MoS2.

[0029] like Figure 1-Figure 2 As shown, Figure 1 There is a morphological picture of the nano adjuvant obtained by the modification treatment of this embodiment. Figure 1 As can be seen in a, CFO@MoS2 is spherical with a diameter of 200-300 nm. Figure 1 It can be seen from the high-resolution TEM images of b and c that CFO@MoS2 has the (100) crystal plane of molybdenum disulfide and the (311) crystal plane of copper iron oxide, indicating that a heterojunction of molybdenum disulfide and copper iron oxide exists in the CFO@MoS2 nanoparticles. Figure 1 d is the element distribution diagram of CFO@MoS2 nanoparticles. It can be seen from the figure that it is mainly composed of Cu, Fe, Mo, S, O and other elements, and the elements are evenly distributed. Figure 2The XRD graph of the nanoadjuvant obtained by the modification treatment of this embodiment is shown in FIG. As can be seen from the figure, the characteristic peaks of CFO@MoS2 are consistent with those of CFO, and the main phase is also copper iron oxide, which is because the content of molybdenum disulfide quantum dots in CFO@MoS2 nanoparticles is relatively low.

[0030] Example 3 300 μL of ultrapure water (control group), CFO, MoS2, and CFO@MoS2 nanoparticles (400 μg / mL) were added to the DCFH-DA solution, and then the DCFH-DA solution was heated at 1.5 W / cm 2 Ultrasonication was performed at a power of 3, 6, 9, 12, and 15 minutes. Subsequently, the reaction solution was centrifuged and 100 μL was taken. Then, the emission wave intensity of the solution at 520 nm was detected under an excitation light of 488 nm.

[0031] like Figure 3 As shown, Figure 3 a is the green fluorescence intensity value of different sample groups and DCFH-DA solution after ultrasound for different time periods. It can be seen from the figure that at all ultrasound time points, the fluorescence intensity of the CFO@MoS2 group is the highest. This shows that under ultrasound stimulation, CFO@MoS2 produces the most oxygen free radicals, followed by the CFO group, MoS2 group, and the blank control group, which are the weakest.

[0032] Example 4 CFO@MoS2 nanoparticles were added to deionized water and the concentration was adjusted to 200 ppm. A magnetic stirrer was used to stir the nanoparticles to ensure uniform dispersion of the nanoparticles. 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO, 50 mM) was added to the CFO@MoS2 solution and then heated at 1.5 W / cm 2 The solution was ultrasonically treated for 15 minutes at a power of 1000 nm. An appropriate amount of the reaction solution was taken out and the signal intensity was recorded using an ESR instrument.

[0033] like Figure 3 As shown, Figure 3 b is the ESR spectrum of CFO@MoS2 nano solution with and without ultrasonic treatment. It can be seen from the figure that after ultrasonic treatment, the signal of hydroxyl radicals is significantly enhanced. This means that ultrasonic stimulation can stimulate CFO@MoS2 nanoparticles to produce hydroxyl radicals.

[0034] Example 5 CFO@MoS2 nanoparticles were added to deionized water and the concentration was adjusted to 200 ppm. A magnetic stirrer was used to stir the nanoparticles to ensure uniform dispersion of the nanoparticles. 2,2,6,6-Tetramethylpiperidine (TEMP, 10 mM) was added to the CFO@MoS2 solution and then heated at 1.5 W / cm2 The solution was ultrasonically treated for 15 minutes at a power of 1000 nm. An appropriate amount of the reaction solution was taken out and the signal intensity was recorded using an ESR instrument.

[0035] like Figure 3 As shown, Figure 3 c is the ESR spectrum of CFO@MoS2 nano solution with and without ultrasonic treatment. As can be seen from the figure, after ultrasonic treatment, the signal of singlet oxygen is significantly enhanced. This means that ultrasonic stimulation can stimulate CFO@MoS2 nanoparticles to produce singlet oxygen.

[0036] Example 6 Gram-positive Staphylococcus aureus was used to evaluate the antibacterial activity of ultrapure water (control group), CFO, MoS2, and CFO@MoS2 nanoparticles (400 μg / mL). 200 μL of bacteria (1×10 8 CFU / mL) was mixed with 200 μL of nanoadjuvant and added to a 24-well plate and heated at 1.5 W / cm 2 The bacteria were then fixed with 2.5% glutaraldehyde and dehydrated with different concentrations of alcohol. Finally, the bacteria were treated with gold spraying and the morphology of the bacteria was observed using SEM.

[0037] The results of this example are as follows Figure 4 As shown. Without ultrasound, the morphology of the four groups of bacteria is round and quite complete. Under ultrasound treatment, the bacterial morphology of the control group and the MoS2 group is still relatively complete, while a small part of the wrinkled area appears on the surface of the bacteria in the CFO group. The integrity of the cell membrane of the bacteria in the CFO@MoS2 group is severely damaged, showing that it has the best bactericidal effect under ultrasound conditions.

[0038] Example 7 Gram-positive Staphylococcus aureus was used to evaluate the antibacterial activity of CFO, MoS2, and CFO@MoS2 nanoparticles (200 μg / mL), and saline was used as the control group. 9 CFU / mL) was mixed with 200 μL of nanoadjuvant and added to a 24-well plate and heated at 1.5 W / cm 2 The cells were ultrasonically treated at a power of 100 for 15 minutes. Then 100 μL of bacterial live / death dye was added, the bacteria were washed after 30 minutes, and the live / death of the bacteria was observed under a fluorescence microscope.

[0039] The results of this example are as follows Figure 5As shown. Without ultrasound, a large number of live bacteria were observed in all four groups of samples. Under ultrasound treatment, the bacteria in the control group and the MoS2 group were still alive, while some areas of the bacteria in the CFO group were dead. The bacteria in the CFO@MoS2 group were basically dead, showing the best bactericidal effect under ultrasound conditions.

[0040] Example 8 C3H10T1 / 2 cells were used to evaluate the cell compatibility of CFO, MoS2, and CFO@MoS2 nanoparticles, and complete culture medium was used as the control group. 1 mL of 5×10 4 cell / mL cell suspension. After the cells adhered for 12 hours, the culture medium was discarded and 1 mL of nanoparticles (200 μg / mL) or complete culture medium was added. The cells were cultured for another 24 hours, stained with a cell death and viability staining reagent, and the cell status was observed under a fluorescence microscope.

[0041] The results of this example are as follows Figure 6 As shown in a. A large number of well-spread cells were observed in all experimental groups, while almost no dead cells were observed. Therefore, the CFO@MoS2 nanoheterojunction did not affect the activity of the cells.

[0042] Example 9 C3H10T1 / 2 cells were used to evaluate the cell compatibility of CFO, MoS2, and CFO@MoS2 nanoparticles, and complete culture medium was used as the control group. 200 μL of 5×10 4 cell / mL cell suspension. After 12 hours of cell adhesion, the culture medium was discarded and 200 μL of nanoparticles (200 μg / mL) or complete culture medium was added. After 1, 3, and 5 days of culture, cell proliferation was detected using the CCK-8 kit.

[0043] The results of this example are as follows Figure 6 As shown in b. There was no significant difference in the activity of cells in all experimental groups at the three detection time points, and the number of cells increased with the extension of time. This shows that CFO@MoS2 nanoheterojunction does not affect cell proliferation.

[0044] Example 10 C3H10T1 / 2 cells were cultured in vitro and qRT-PCR was used to evaluate the effects of CFO, MoS2, and CFO@MoS2 nanoparticles on the osteogenic differentiation of stem cells. 1 mL of 5×10 4cell / mL cell suspension. After the cells adhered for 12 hours, the medium was aspirated and 1 mL of nanoparticles (200 μg / mL) or complete medium was added. After 3 days and 7 days of culture, the medium was aspirated, the cells were lysed with Trizol, and the RNA purity was detected with a Nanodrop spectrophotometer. Then, the cDNA first-strand synthesis kit was used for reverse transcription reaction, and the obtained cDNA was stored at -20 ℃. Finally, the cDNA was mixed with SYBR Green Mastermix and primers and 2 -ΔΔct Methods The relative gene expression of target genes was calculated.

[0045] like Figure 7 As shown, Figure 7 The expression of osteogenic-related genes in the above samples after co-culture with C3H10T1 / 2 for 3 and 7 days. As can be seen from the figure, for the four genes tested, including RUNX2, ALP, OCN, and OPN, the expression levels of the CFO@MoS2 group are the highest, indicating that it is most conducive to the osteogenic differentiation of stem cells.

[0046] Embodiment 11 HUVECs cells were cultured in vitro and qRT-PCR was used to evaluate the effects of CFO, MoS2, and CFO@MoS2 nanoparticles on endothelial cell angiogenesis. 1 mL of 5×10 4 cell / mL cell suspension. After the cells adhered for 12 hours, the medium was aspirated and 1 mL of nanoparticles (200 μg / mL) or complete medium was added. After 3 days and 7 days of culture, the medium was aspirated, the cells were lysed with Trizol, and the RNA purity was detected with a Nanodrop spectrophotometer. Then, the cDNA first-strand synthesis kit was used for reverse transcription reaction, and the obtained cDNA was stored at -20 ℃. Finally, the cDNA was mixed with SYBR Green Mastermix and primers and 2 -ΔΔct Methods The relative gene expression of target genes was calculated.

[0047] like Figure 8 As shown, Figure 8 The expression of angiogenesis-related genes after the above samples were co-cultured with HUVECs for 3 days and 7 days. As can be seen from the figure, for the three genes tested, including HIF-1, VEGF, and PDGF, the expression levels of the CFO@MoS2 group were the highest, indicating that it is most conducive to the angiogenesis of endothelial cells.

[0048] Based on the results of Examples 3-11 above, it can be seen that CFO@MoS2 nanoheterojunction can produce reactive oxygen species such as hydroxyl radicals and singlet oxygen under ultrasonic stimulation, thereby killing Staphylococcus aureus. At the same time, CFO@MoS2 nanoheterojunction material not only has good cell compatibility, but also promotes osteogenic differentiation of stem cells and vascularization of endothelial cells. The copper iron oxide and molybdenum disulfide quantum dot nanoheterojunction materials prepared by the invention are expected to be widely used in the treatment of bone infections or other bacteria-related diseases.

[0049] It should be understood that the above specific embodiments of the present invention are only used to illustrate or explain the principles of the present invention, and do not constitute a limitation of the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc. made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. In addition, the appended claims of the present invention are intended to cover all changes and modifications that fall within the scope and boundaries of the appended claims, or the equivalent forms of such scope and boundaries.

Claims

1. An ultrasonically responsive nanoheterojunction material, characterized in that: The invention comprises CuFe2O4 nanoparticles and MoS2 quantum dots, wherein the MoS2 quantum dots are adsorbed on the CuFe2O4 nanospheres.

2. An ultrasonically responsive nanoheterojunction material, characterized in that: Its chemical formula is CuFe2O4•0.12MoS2.

3. The method for preparing an ultrasonic responsive nano heterojunction material according to any one of claims 1 to 2, characterized in that: The following steps are involved: Step 1: Add copper salt and iron salt to ethylene glycol respectively and dissolve them by ultrasonication. The copper salt is at least one of copper chloride, copper nitrate and copper sulfate, and the iron salt is at least one of iron chloride, iron nitrate and iron sulfate; Step 2: add sodium acetate to the mixed solution obtained in step 1 and stir evenly. In the presence of sodium acetate and oxygen, Cu 2+ With Fe 3+ Combines with O2 to form CuFe2O4; Step 3, adding molybdenum disulfide quantum dots to the mixed solution obtained in step 2, and stirring evenly; Step 4, pouring the mixed solution obtained in step 3 into a polytetrafluoroethylene reactor, and placing a stainless steel outer lining, and reacting in an oven for a period of time, the molybdenum disulfide quantum dots are negatively charged, the CuFe2O4 is positively charged, and the molybdenum disulfide quantum dots are electrostatically adsorbed to the surface of CuFe2O4 to form a nano heterojunction of MoS2 and CuFe2O4; Step 5, placing the reaction mixture in a centrifuge for centrifugation, then adding deionized water, and ultrasonically dispersing it, and then placing it in a centrifuge for centrifugation again to remove the supernatant; Step six, placing the product obtained in step five into a vacuum drying oven for drying.

4. The method for preparing an ultrasonic responsive nano heterojunction material according to claim 3, characterized in that In step 1, the copper salt used is copper chloride, the iron salt used is ferric chloride, and the concentration of copper chloride is 4.25 mg / mL, and the concentration of ferric chloride is 13.5 mg / mL.

5. The method for preparing an ultrasonic responsive nano heterojunction material according to claim 3, characterized in that: The amount of sodium acetate added in step 2 is 25 mg / mL.

6. The method for preparing an ultrasonic responsive nano heterojunction material according to claim 3, characterized in that: The molybdenum disulfide quantum dots added in step three were 3.75 mg / mL.

7. The method for preparing an ultrasonic responsive nano heterojunction material according to claim 3, characterized in that The reaction temperature of step 4 is 180° C. and the reaction time is 24 hours.

8. The method for preparing an ultrasonic responsive nano heterojunction material according to claim 3, characterized in that: In step 5, the mixed solution is placed in a 10,000 g centrifuge for 10 minutes, the supernatant is removed, and then deionized water is added and ultrasonically dispersed, and then centrifuged in a 10,000 g centrifuge for 10 minutes, and the supernatant is removed.

9. The use of an ultrasonic responsive nano heterojunction material according to any one of claims 1 to 2, characterized in that: Application of the nano heterojunction material in bone infection or other clinical infections.

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