Yeast source functional feed for promoting sheep growth and reducing diseases

Abstract

The invention relates to the technical field of animal husbandry, and discloses a yeast source functional feed for promoting sheep growth and reducing diseases. The yeast source functional feed comprises 36-39 parts of corn, 13-17 parts of soybean meal, 6-7 parts of corn germ meal, 10-32 parts of corn bran, 0-16 parts of peanut shells, 0-0.5 part of corn oil, 4-5 parts of bentonite, 4-5 parts of cane sugar, 5-6 parts of a premix and a feed additive. The active ingredients of the feed additive comprise hemicellulose with the content of 10%-17% DM and mannan with the content of 80 mg / kg-320 mg / kg DM. The yeast source functional feed for promoting the growth of the sheep and reducing the diseases is adopted, so that the slaughter performance of the sheep can be improved; meanwhile, after mannan is added, the blood fat, anti-inflammatory and anti-tumor capabilities can be improved, and the disease resistance of the sheep is improved; the meat quality can be regulated and controlled, lipid metabolism is promoted, and fatty acid is catalyzed to be oxidized to generate lipid.

Description

Technical Field

[0001] The present invention relates to the technical field of animal husbandry, and in particular to a yeast-derived functional feed for promoting the growth of sheep and reducing diseases. Background Art

[0002] Feed mainly refers to the food for animals raised in agriculture or animal husbandry. Feed includes feed raw materials of multiple varieties such as soybeans, soybean meal, corn, amino acids, miscellaneous meal, whey powder, grains, feed additives, etc. Current animal feeds are difficult to effectively care for and strengthen the gastrointestinal health and disease resistance of ruminants, and ruminants are not easily absorbent to macromolecular substances in the feed, thereby greatly reducing the growth-promoting effect that the feed can have on ruminants. In order to improve animal production performance and ensure animal health, it is inevitable to add feed additives during feed production. Feed additives refer to small amounts or trace substances added during the feed production, processing, and use processes. They have a small dosage in the feed but a significant effect. Feed additives are essential raw materials in modern feed industry, and have obvious effects in aspects such as strengthening the nutritional value of basic feed, improving animal production performance, ensuring animal health, saving feed costs, and improving the quality of livestock products. Currently, feed additives are mainly nutritional substance additives containing minerals, vitamins, amino acids, etc.; antibiotic substances for preventing diseases; hormonal additives, which are regulators for accelerating the rapid growth of animals. However, these additives will cause drug residues and enhanced drug resistance. To solve the above problems, the present invention provides a yeast-derived functional feed that does not add antibiotics and can promote the growth of sheep and reduce diseases. Summary of the Invention

[0003] The purpose of the present invention is to provide a yeast-derived functional feed for promoting the growth of sheep and reducing diseases, which can improve the slaughter performance of sheep; at the same time, after adding mannan, it can improve blood lipid, anti-inflammatory and anti-tumor abilities, and improve the disease resistance of sheep; it can also regulate meat quality, promote lipid metabolism, and catalyze fatty acid oxidation to produce lipids.

[0004] To achieve the above purpose, the present invention provides a feed additive, and the active ingredients include hemicellulose with a content of 10%-17% DM and mannan with a content of 80-320 mg / kg DM.

[0005] Further, the active ingredients of the feed additive include hemicellulose with a content of 10.3% DM and mannan with a content of 160 mg / kg DM.

[0006] The present invention also provides the application of the above feed additive in the preparation of a yeast-derived functional feed for promoting the growth of sheep and reducing diseases.

[0007] The present invention also provides a yeast-derived functional feed for promoting the growth of sheep and reducing diseases, comprising the following components in parts by weight: 36-39 parts of corn, 13-17 parts of soybean meal, 6-7 parts of corn germ meal, 10-32 parts of corn bran, 0-16 parts of peanut hulls, 0-0.5 parts of corn oil, 4-5 parts of bentonite, 4-5 parts of sucrose, a 5-6 parts of premix and the above-mentioned feed additive.

[0008] Further, the yeast-derived functional feed comprises the following components in parts by weight: 38 parts of corn, 16.5 parts of soybean meal, 6.5 parts of corn germ meal, 10.5 parts of corn bran, 15.5 parts of peanut hulls, 0.5 parts of corn oil, 4 parts of bentonite, 4 parts of sucrose, a 5 parts of premix and the above-mentioned feed additive.

[0009] Further, a Each kilogram of the premix contains: FeSO 4 179 mg, CuSO 4 ·5H 2 O 23 mg, ZnSO 4 ·5H 2 O 92 mg, MnSO 4 70 mg, vitamin A 16 KIU, vitamin D 111 KIU, vitamin E 915 IU.

[0010] Further, the nutrient components in the feed are: dry matter 90-91% DM, digestible energy 11-13% DM, crude protein 13.8-14% DM, crude fat 2.7-3% DM, crude fiber 6-13.1% DM, crude ash 12.5-14.6% DM, starch 30.1-30.5% DM, neutral detergent fiber 25-26.0% DM, acid detergent fiber 9-15.7% DM, hemicellulose 10.3-17% DM, calcium 0.6% DM, phosphorus 0.4% DM.

[0011] The present invention also provides the application of the above-mentioned yeast-derived functional feed for promoting the growth of sheep and reducing diseases in improving the meat quality and slaughter rate of sheep.

[0012] The present invention also provides the application of the above-mentioned yeast-derived functional feed for promoting the growth of sheep and reducing diseases in regulating the balance of the sheep flora.

[0013] The present invention also provides the application of the above-mentioned yeast-derived functional feed for promoting the growth of sheep and reducing diseases in reducing inflammation and improving the disease resistance of sheep.

[0014] The advantages and positive effects of the yeast-derived functional feed for promoting the growth of sheep and reducing diseases according to the present invention are:

[0015] 1. By adding mannan to the diet, the present invention can improve the shear force and cooked meat rate, improve the meat quality, reduce the water loss rate, and improve the slaughter performance of sheep.

[0016] 2. The feed in the present invention does not add antibiotics. By adding mannan, it can improve blood lipid, anti-inflammatory and anti-tumor abilities, and thus improve the disease resistance of sheep.

[0017] 3. After adding mannan, the present invention can regulate the meat quality, promote lipid metabolism, and catalyze fatty acid oxidation to produce lipids.

[0018] The technical solution of the present invention will be further described in detail below through the drawings and embodiments. Description of the Drawings

[0019] Figure 1 It is the differential gene expression levels screened in the embodiments of the present invention. Detailed Embodiments

[0020] The technical solution of the present invention will be further described below through the drawings and embodiments.

[0021] Unless otherwise defined, the technical terms or scientific terms used in the present invention shall have the ordinary meanings understood by those of ordinary skill in the art to which the present invention belongs.

[0022] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. The experimental methods without specific conditions noted in the following embodiments are usually determined according to national standards. The experimental instruments, equipment and reagents not indicated in the following embodiments are all commercially available raw materials.

[0023] Unless otherwise defined or stated, all the professional and scientific terms used in the present invention have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the method of the present invention.

[0024] Example 1 Screening the Optimal Mannan Content in Vitro

[0025] Diet formula (Table 1):

[0026] Table 1 Diet formula

[0027]

[0028] a Premix ingredients (per kilogram): FeSO 4 179 mg, CuSO 4 ·5H 2O 23 mg, ZnSO 4 ·5H 2 O 92 mg, MnSO 4 70 mg, vitamin A 16 KIU, vitamin D 111 KIU, vitamin E 915 IU.

[0029] Functional yeast-derived feeds with different levels of mannan were added to the diet to screen for the optimal addition amount of mannan.

[0030] The in vitro screening steps are as follows:

[0031] 1. Experimental animals and location:

[0032] Four healthy and quarantined male Small Tail Han sheep rams (36 ± 1.5 kg) were selected as experimental animals, and permanent rumen fistula installation surgery was performed. After the surgery, the health status of the sheep was observed regularly, the fistula was cared for and cleaned. After the surgical wound healed and the sheep's condition recovered, the experiment was carried out. All experimental animals were housed individually in cages, the fistula was cleaned regularly and the health status of the experimental animals was observed, and the sheep house was cleaned regularly to maintain hygiene. The experimental animals were fed at 8:00 am and 5:00 pm every day and were allowed to drink fresh and clean water ad libitum. The experiment was conducted at the Research and Teaching Base West of Jilin Agricultural University, and all experimental procedures were strictly carried out in accordance with the "Guidelines for the Care and Use of Laboratory Animals at Jilin Agricultural University".

[0033] 2. Collection of rumen fluid:

[0034] On the day of the experiment, CO 2 was introduced into a thermos preheated to 39 °C for two minutes to ensure a suitable living environment for anaerobic microorganisms in the rumen fluid. Before the morning feeding of the fistulated sheep, mixed rumen fluid was collected from different parts of the rumen using a rigid PVC tube. The collected rumen fluid was quickly placed into a thermos, the bottle cap was tightened, and it was immediately returned to the laboratory. The rumen fluid was filtered through multiple layers of gauze. Throughout the operation process, the temperature was ensured to be appropriate and CO 2 was continuously introduced to ensure an anaerobic environment.

[0035] 3. In vitro culture:

[0036] The gas production was monitored and recorded using an ANKOM RFS device. Before the experiment, 2.000 g of substrate (the diet formula in Table 1) was placed into an 800-mesh nylon bag, sealed with a sealer, and placed in an ANKOM bottle, one bag in each bottle. Mannan was added to the bottle. The rumen fluid and buffer were added to the bottle in a ratio of 1:2, and CO 2 was continuously introduced into the bottle for one minute. The bottle cap was quickly tightened and placed into a 39 °C constant temperature air bath incubator for shaking culture (80 rpm).

[0037] Buffer preparation method: 1 hour before the start of the experiment, mix Solution A, Solution B, and Solution C evenly in a ratio of 494:5:1 according to the required amount of the experiment. After mixing, continuously introduce CO 2 to the bottom of the buffer until the buffer turns colorless. Then, place the buffer in a water bath at 39 °C for preheating and standby.

[0038] Formula of Solution A: K 2 HPO 4 382.5 mg / L, KH 2 PO 4 292 mg / L, (NH4) 2 SO 4 480 mg / L, NaCl 200 mg / L, MgSO 4 ·7H 2 O 100 mg / L, Na 2 CO 3 4000 mg / L.

[0039] Formula of Solution B: EDTA 500 mg / L, FeSO 4 ·7H 2 O 200 mg / L, MnCl 2 ·4H 2 O 200 mg / L, ZnSO 4 ·7H 2 O 10 mg / L, H 3 BO 3 30 mg / L, CoCl 2 ·6H 2 O 20 mg / L, CuCl 2 ·2H 2 O 1 mg / L, NiCl 2 ·6H 2 O 2 mg / L, NaMoO 4 3 mg / L.

[0040] Formula of Solution C: Na 2 S·9H 2 O 25 g / 100 mL.

[0041] Solution A is prepared one day before the experiment. After preparing Solution B, CO 2 needs to be continuously introduced for 18 hours. After sealing, it can be stored in the refrigerator for long-term use. After preparing Solution C, CO 2 needs to be continuously introduced for 20 minutes. After sealing, it can be stored in the refrigerator for long-term use.

[0042] 4. NH 3 -N concentration determination:

[0043] For the determination of ammonia nitrogen in rumen fluid by colorimetry, colorimetric determination was carried out using an ultraviolet spectrophotometer.

[0044] 5. Determination of VFA:

[0045] The determination of volatile fatty acids was carried out using a gas chromatograph.

[0046] 6. Determination of in vitro fermentation gas production:

[0047] The gas production was determined using the ANKOM RFS device, and the data of gas production in the fermentation bottle were saved and recorded by using the software GPM.

[0048] 7. Determination of in vitro nutrient disappearance rate:

[0049] The nylon bag containing the substrate after fermentation in in vitro culture was taken out of the bottle, washed repeatedly, and the rumen fluid on the bag was washed until the water became clear. After the bag was air-dried, it was put into an oven and dried at 105 °C to constant weight, and the dry matter (DM) in the feed raw material was determined, and the in vitro dry matter disappearance rate (IVDMD) was calculated.

[0050] 2. Result analysis:

[0051] 2.1 In vitro fermentation nutrient disappearance rate:

[0052] Table 2 Nutrient digestibility

[0053]

[0054] As can be seen from Table 2, after 6 h of in vitro fermentation, adding different doses of mannan had a highly significant effect on IVDMD. Among them, the mannan added at 160 mg / kg DM was significantly higher than the control group and other experimental groups, while the mannan added at 320 mg / kg DM was significantly lower than the 160 mg / kg DM dose group.

[0055] 2.2 In vitro fermentation VFA:

[0056] Table 3 Volatile fatty acid concentration

[0057]

[0058] Table 3 shows the effects of adding different doses of mannan in the diet on VFA concentration after 6 h of in vitro fermentation. As can be seen from Table 3, after 6 h of in vitro fermentation, the total acid (TVFA) content in the group adding 160 mg / kg DM mannan was highly significantly higher than that in the control group and the groups adding 80 and 320 mg / kg DM mannan. Different levels of hemicellulose in the diet had a significant effect on the total acid, adding different doses of mannan had a highly significant effect on it, and the interaction effect between the two had a significant effect on it.

[0059] 2.3 In vitro fermentation of ammonia nitrogen NH 3 -N concentration and gas production:

[0060] Table 4

[0061]

[0062] Table 4 shows the effects of adding different doses of mannan in the diet on ammonia nitrogen concentration and gas production during 6 h of in vitro fermentation. As can be seen from Table 4, during 6 h of in vitro fermentation, the concentration of ammonia nitrogen (NH 3 -N) decreased significantly, and the gas production increased significantly. Through comprehensive analysis, the optimal addition amount of mannan is 160 mg / kg DM.

[0063] Example 2 In vivo experiment

[0064] Analyze the effects of feeding the diet and adding mannan on the growth performance and muscle indexes of sheep.

[0065] I. Growth performance:

[0066] 1. Materials and methods:

[0067] 1.1 Select 24 healthy and quarantined male Small Tail Han sheep rams (30 ± 1.5 kg) as experimental animals, divide them into a group without adding mannan (LHM0) and a group adding mannan (LHM160), with 3 replicates in each group and 2 sheep in each replicate. The experimental sheep are raised in cages with each replicate as one cage, and are dewormed and tested for brucellosis before feeding. The pre-feeding period is 12 days, and the formal feeding period is divided into 3 stages, each stage being 24 days for a total of 72 days, and slaughtering is carried out on the 85th day. The experiment is carried out at the Daoxi Scientific Research and Teaching Base of Jilin Agricultural University, and all experimental steps are strictly implemented in accordance with the "Guidelines for the Care and Use of Laboratory Animals of Jilin Agricultural University".

[0068] 1.2 Growth performance determination:

[0069] On the morning of the first day of the formal experiment, the animals are weighed on an empty stomach, and then weighed on an empty stomach every 24 days. Record the body weight and calculate the weight gain rate and total weight gain.

[0070] 1.3 Collection of the longissimus dorsi muscle:

[0071] After slaughter, all the longissimus dorsi muscles are dissected. Part of them is used for the determination of meat quality, and the rest is stored in a -80 °C refrigerator for subsequent detection of muscle indexes.

[0072] 1.4 Meat quality determination:

[0073] Meat color: Take the longissimus dorsi muscle for measurement. Use a fully automatic color difference meter to measure the color of the longissimus dorsi muscle according to the usage method in the instruction manual. Measure the meat color values (brightness L*, redness a*, yellowness b*) three times and take the average value.

[0074] Shearing force: Take a small piece of the longissimus dorsi muscle using a shear instrument, measure its shearing force three times, and finally take the average value.

[0075] Cooked meat rate: Take about 100 g of the psoas major muscle from the carcass, remove the outer membrane fat, weigh it as W1 using an analytical balance, put it in an electric furnace with a power of 2000 watts and steam it for 45 minutes, take it out and cool it for 30 minutes, and then weigh it again as W2. Calculate its cooked meat rate.

[0076] The formula for calculating the cooked meat rate is: Cooked meat rate = W2 / W1 × 100%.

[0077] 2. Result analysis:

[0078] 2.1 Growth performance of sheep:

[0079] As shown in Table 5, the effects of adding mannan to the diet on the growth performance of sheep are presented. In the group fed with a low hemicellulose diet, the total weight gain and weight gain rate of the sheep in the group added with mannan (LHM160) were significantly higher than those in the non-added group (LHM0) (P < 0.05).

[0080] Table 5 Growth performance

[0081] LHM0 LHM160 Total weight gain (TWG) <![CDATA[17.75 b > <![CDATA[22.33 a > Weight gain rate (WG%) <![CDATA[36.58 b > <![CDATA[41.71 a >

[0082] 2.2 Meat quality of sheep:

[0083] Table 6 Meat quality

[0084]

[0085]

[0086] Table 6 shows the effects of adding mannan to the diet on the meat quality of sheep after slaughter. After feeding the diet and adding mannan, there were no significant differences in meat color (brightness, redness, yellowness). However, the shearing force and cooked meat rate in the LHM160 group were higher than those in the LHM0 group; the water loss rate in the LHM160 group was lower than that in the LHM0 group, improving the meat quality. Generally speaking, supplementing mannan in the diet can improve the growth performance of animals.

[0087] II. Muscle indicators:

[0088] 1.1 Collection of the longissimus dorsi muscle:

[0089] After slaughtering the experimental sheep, take the longissimus dorsi muscles on both sides. Part of them is used to measure the meat quality, and the remaining part is used to measure the intramuscular fat.

[0090] 1.2 Determination of fatty acids:

[0091] Take an appropriate amount of the sample in a centrifuge tube, add 4 mL of CHCl 3 solution, vortex and mix well, then add 0.9% NaCl solution, mix and centrifuge again (3500 rpm, 15 min). Take the lower layer solution to a new centrifuge tube, add 2 mL of CH 3 Cl 2 , vortex and mix well again. After centrifuging for 15 min, take the lower layer solution. Repeat the extraction twice, mix the two lower layer solutions, dry them under a nitrogen stream, and continue to add 2 mL of CH 3 OH solution (containing 5% H 2 SO 4 solution), vortex and mix well, water bath at 80 °C for 2 h. After cooling, add 2 mL of n-hexane solution and 1 mL of water, vortex and mix well for 30 s, then centrifuge (2000 rpm, 5 min). Take the supernatant again, add 1 mL of water, vortex and mix well for 30 s, then centrifuge (2000 rpm, 5 min). Take the supernatant and dry it under a nitrogen stream, add an appropriate volume of isooctane solution, vortex and let it stand, and then perform on-machine detection. The instrument used is an Agilent gas chromatography system (Agilent 7820, Agilent Technologies, USA).

[0092] 1.3 Result analysis:

[0093] Table 7 shows the concentrations of medium- and long-chain fatty acids in the longissimus dorsi muscle of sheep fed with diets supplemented with mannan. Among them, oleic acid, palmitic acid, stearic acid, linoleic acid, trans-9-octadecenoic acid, arachidonic acid, myristic acid, and palmitoleic acid have the highest proportions.

[0094] Table 7 Concentrations of fatty acids (μg / mg)

[0095] LHM0 LHM160 Caprylic acid <![CDATA[0.0004 ab > <![CDATA[0.0008 a > Capric acid <![CDATA[0.0095 b > <![CDATA[0.0134 a > Lauric acid 0.0089 0.0085 Myristic acid 0.2152 0.1800 Myristoleic acid 0.0078 0.0061 Pentadecanoic acid 0.0238 0.0207 Palmitic acid 3.1636 2.6327 Palmitoleic acid 0.1857 0.1548 Margaric acid 0.1002 0.0829 Heptadecenoic acid 0.0455 0.0379 Stearic acid 1.8272 1.5783 trans-9-Octadecenoic acid 0.5302 0.4497 Oleic acid 4.4315 3.7451 Linoleic acid 1.3922 1.2272 11-Eicosenoic acid <![CDATA[0.0184 ab > <![CDATA[0.0173 b > Behenic acid <![CDATA[0.0032 a > <![CDATA[0.0032 a > Erucic acid <![CDATA[0.0264 a > <![CDATA[0.0210 b > Tricosanoic acid <![CDATA[0.0016 a > <![CDATA[0.0014 ab > Arachidonic acid 0.3499 0.3078

[0096] It can be seen from Table 7 that after adding mannan to the diet, the contents of caprylic acid and capric acid increase. Caprylic acid can reduce body fat, improve blood lipid levels, inhibit the production of inflammatory cytokines, and alleviate atherosclerosis in mice; capric acid can promote the activation of anti-tumor cells, enhance the anti-tumor immune ability of the body, and at the same time capric acid can keep the intestinal flora of mice uniform and maintain the normal vitality of the intestinal flora. Therefore, adding mannan to the diet can improve blood lipids, increase anti-inflammatory and anti-tumor abilities, and at the same time regulate the balance of the intestinal flora.

[0097] 1.5 Screening of differential genes:

[0098] A total of 125 differential genes were detected in the LHM0 vs LHM160 group, with 59 up-regulated and 66 down-regulated.

[0099] In the LHM0 vs LHM160 group, the differentially expressed genes were mainly enriched in pathways such as the AMPK signaling pathway, Insulin signaling pathway, Glucagon signaling pathway, Glycolysis / Gluconeogenesis, Metabolism of xenobiotics by cytochrome P450, Drug metabolism-cytochrome P450, Drug metabolism-other enzymes, Glutathione metabolism, Butanoate metabolism, Arginine and proline metabolism, and Purine metabolism.

[0100] According to the enrichment pathways of differentially expressed genes, as shown in Table 8, 13 genes related to sugar and lipid metabolism were selected for subsequent qPCR verification.

[0101] Table 8 Related differentially expressed genes

[0102]

[0103] qPCR verification:

[0104] The primer sequence information used is shown in Table 9.

[0105] Table 9

[0106]

[0107] Figure 1For the differential gene expression levels screened out, in the LHM0 vs LHM160 group, the PPP2R2C gene in the LHM0 group was lower than that in the LHM160 group, and the SIK1 and GAPDH genes were higher than those in the LHM160 group. The PPP2R2C gene is highly correlated with lipids, participates in the regulation of meat quality, and is related to oxidized lipids, carnitine, and ceramides; the SIK1 gene can inhibit lipid synthesis; GAPDH plays a major enzymatic role in intermediate metabolism, and cells in almost all organisms require the catalytic ability of GAPDH to maintain sufficient glycolysis. Mannan was added to the LHM160 group, resulting in an increase in the expression of the PPP2R2C gene and a decrease in the expression of the SIK1 and GAPDH genes, which can regulate meat quality, promote lipid metabolism, and catalyze fatty acid oxidation to produce lipids.

[0108] In summary, by adding mannan to the diet, the present invention can improve shear force and cooked meat rate, improve meat quality, reduce water loss rate, and improve the slaughter performance of sheep; at the same time, after adding mannan, it can improve blood lipid, increase anti-inflammatory and anti-tumor abilities, and improve the disease resistance of sheep; it can also regulate meat quality, promote lipid metabolism, and catalyze fatty acid oxidation to produce lipids.

[0109] Therefore, the yeast-derived functional feed of the present invention that promotes the growth of sheep and reduces diseases can improve the slaughter performance of sheep; at the same time, after adding mannan, it can improve blood lipid, anti-inflammatory and anti-tumor abilities, and improve the disease resistance of sheep; it can also regulate meat quality, promote lipid metabolism, and catalyze fatty acid oxidation to produce lipids.

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that they can still modify or equivalently replace the technical solutions of the present invention, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A feed additive, characterized in that: The effective components include hemicellulose with a content of 10%-17% DM and mannan with a content of 80-320 mg / kg DM.

2. A feed additive according to claim 1, characterized in that: The effective ingredients include hemicellulose with a content of 10.3% DM and mannan with a content of 160 mg / kg DM.

3. Use of the feed additive according to claim 1 or 2 in preparing yeast-derived functional feed for promoting sheep growth and reducing diseases.

4. A yeast-derived functional feed for promoting sheep growth and reducing diseases, characterized in that: The composition includes the following components by weight: 36-39 parts of corn, 13-17 parts of soybean meal, 6-7 parts of corn germ meal, 10-32 parts of corn husk, 0-16 parts of peanut shell, 0-0.5 parts of corn oil, 4-5 parts of bentonite, 4-5 parts of sucrose, a 5-6 parts of premix and the feed additive according to claim 1.

5. The yeast-derived functional feed for promoting sheep growth and reducing diseases according to claim 4, characterized in that: The composition includes the following components by weight: 38 parts of corn, 16.5 parts of soybean meal, 6.5 parts of corn germ meal, 10.5 parts of corn husk, 15.5 parts of peanut shell, 0.5 parts of corn oil, 4 parts of bentonite, 4 parts of sucrose, a 5 parts of premix and feed additives.

6. The yeast-derived functional feed for promoting sheep growth and reducing diseases according to claim 4, characterized in that: Said a The components per kilogram of premix are: FeSO4 179 mg, CuSO4·5H2O 23 mg, ZnSO4·5H2O 92 mg, MnSO4 70 mg, vitamin A 16 KIU, vitamin D 111 KIU, and vitamin E 915 IU.

7. The yeast-derived functional feed for promoting sheep growth and reducing diseases according to claim 4, characterized in that: The nutritional components of the feed are: dry matter 90-91% DM, digestible energy 11-13% DM, crude protein 13.8-14% DM, crude fat 2.7-3% DM, crude fiber 6-13.1% DM, crude ash 12.5-14.6% DM, starch 30.1-30.5% DM, neutral detergent fiber 25-26.0% DM, acid detergent fiber 9-15.7% DM, hemicellulose 10.3-17% DM, calcium 0.6% DM, and phosphorus 0.4% DM.

8. Use of the yeast-derived functional feed for promoting sheep growth and reducing diseases as claimed in claim 4 in improving sheep meat quality and slaughter rate.

9. Use of the yeast-derived functional feed for promoting sheep growth and reducing diseases as claimed in claim 4 in regulating the balance of sheep flora.

10. Use of the yeast-derived functional feed for promoting sheep growth and reducing diseases as claimed in claim 4 in reducing inflammation and improving sheep's disease resistance.

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