Stereotactic laboratory mouse cerebrospinal fluid automatic extraction device and control system thereof

Abstract

The invention relates to the technical field of medical experimental instruments, and discloses a stereotactic experimental mouse cerebrospinal fluid automatic extraction device which comprises a supporting seat, a supporting frame is fixedly connected to the top of the supporting seat, a first air cylinder is fixedly connected to the outer wall of the supporting frame, and the output end of the first air cylinder penetrates through the outer wall of the supporting frame and is fixedly connected with an extraction pipe. A second air cylinder is fixedly connected to the inner wall of the supporting seat, a first sliding groove is formed in the outer wall of the supporting seat, an adjusting mechanism is arranged in the supporting seat, the positioning mechanism is arranged in the supporting seat and comprises a positioning block, and a first supporting rod is fixedly connected to the outer wall of the positioning block. The mouse is positioned through the positioning mechanism, and the head of the mouse is clamped, so that the head of the mouse is fixed, the stability of the cerebrospinal fluid extraction process is improved, the operation difficulty is reduced, and the extraction device can extract the cerebrospinal fluid of the mouse more stably.

Description

Technical Field

[0001] The present invention relates to the technical field of medical experimental instruments, and particularly to a stereotactic automatic cerebrospinal fluid extraction device for experimental mice and its control system. Background Art

[0002] The method for extracting cerebrospinal fluid from mice relies on manual operation, with low efficiency and being easily affected by the technical level of the operator. To improve the standardization and repeatability of experiments, developing a stereotactic automatic cerebrospinal fluid extraction device for experimental mice has become an industry trend. This device realizes the efficient and accurate collection of cerebrospinal fluid by precisely controlling the body position and puncture process of mice, meets the needs of large-scale experiments, and promotes the progress in the fields of neuroscience research and drug development.

[0003] The patent application with the application number CN202111127513.5 discloses a multifunctional automatic lumbar puncture bed, including a hospital bed body. The hospital bed body includes a bed board and a bed frame, and also includes a urine and feces receiving device, a knee limiting device, a back pressing device, a puncture implementation device, a data acquisition system, and a data processing system. An opening is provided on the bed board, and the urine and feces receiving device includes a sealing plate, a sealing driving mechanism, a collection box, and a receiving driving mechanism.

[0004] However, during the process of extracting cerebrospinal fluid by the automatic cerebrospinal fluid extraction device for mice, since the head of the mouse needs to be tilted slightly downward, the cerebrospinal fluid of the mouse cannot flow down to the collection point, affecting the extraction efficiency and sample quality, and reducing the reliability of experimental data. Summary of the Invention

[0005] The purpose of the present invention is to provide a stereotactic automatic cerebrospinal fluid extraction device for experimental mice and its control system to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A stereotactic automatic cerebrospinal fluid extraction device for experimental mice, including a support base. A support frame is fixedly connected to the top of the support base. A cylinder one is fixedly connected to the outer wall of the support frame. The output end of the cylinder one penetrates through the outer wall of the support frame and is fixedly connected to an extraction tube. A cylinder two is fixedly connected to the inner wall of the support base. A support plate one is slidably connected to the inner wall of the support base. A guide rod is fixedly connected to the inner wall of the support base. A limiting plate is fixedly connected to the outer wall of the support base. A guide groove one is opened on the inner wall of the limiting plate. A sliding groove one is opened on the outer wall of the support base. An adjusting mechanism is arranged inside the support base, and further includes: Positioning mechanism, the positioning mechanism is arranged inside the support base, the positioning mechanism includes a positioning block, a support rod I is fixedly connected to the outer wall of the positioning block, a guide sleeve is slidably connected to the outer wall of the support rod I, an adjustment block is fixedly connected to the outer wall of the guide sleeve, the inner wall of the adjustment block is slidably connected to the groove wall of a sliding groove II opened on the outer wall of a guide plate through a slider, a support rod II is fixedly connected to the outer wall of the guide plate, the outer wall of the support rod II is slidably connected to the groove wall of a guide groove II opened on the outer wall of a support plate III, a support block is fixedly connected to the outer wall of the positioning block, the outer wall of the support block is slidably connected to the groove wall of a sliding groove III opened on the outer wall of a clamp through a slider, the support rod I is used for positioning the head of a mouse, and the clamp is used for fixedly clamping the head of the mouse.

[0007] According to the above technical solution, a spring III is fixedly connected to the outer wall of the support rod I, the other end of the spring III is fixedly connected to the inner wall of the adjustment block, a spring II is fixedly connected to the groove wall of the guide groove II, the other end of the spring II is fixedly connected to the outer wall of the support rod II, and the guide groove II is used for guiding the support rod II.

[0008] According to the above technical solution, the outer wall of the support plate III is fixedly connected to the output pipe of a cylinder III passing through a sliding groove I, the bottom of the cylinder III is fixedly connected to a support plate II, a limiting rod is fixedly connected to the bottom of the support plate II, a spring I is fixedly connected to the bottom of the support plate II, the other end of the spring I is fixedly connected to the inner wall of a limiting plate, and the limiting rod is used for supporting the vertical positioning of the head of the mouse and compensating for the vertical displacement generated after clamping the head of the mouse.

[0009] According to the above technical solution, the adjusting mechanism includes a support plate IV, the inner wall of the support plate IV is slidably connected to the outer wall of a guide rod, a cylinder IV is fixedly connected to the outer wall of the support plate IV, the output end of the cylinder IV passes through the outer wall of the support plate IV and is fixedly connected to the outer wall of an adjusting plate, a support plate V is slidably connected to the inner wall of the support plate IV, a spring IV is fixedly connected to the bottom of the support plate V, the other end of the spring IV is fixedly connected to the inner wall of the support plate IV, the adjusting plate is used for deflecting the head of the mouse, and the support plate V is used for supporting the head of the mouse.

[0010] According to the above technical solution, the outer wall of the adjustment block is hinged to the outer wall of the clamp through a rotating shaft, the number of the support rods II is two groups, the two groups of the support rods II are symmetrically arranged on the outer wall of the guide plate with the center line of the guide plate as the axis of symmetry, the number of the clamps is two groups, the two groups of the clamps are symmetrically arranged on the outer wall of the adjustment block with the center line of the adjustment block as the axis of symmetry, and the adjustment block is used for compensating for the horizontal displacement generated after clamping the head of the mouse and supporting the clamp after fixedly clamping the head of the mouse.

[0011] According to the above technical solution, the number of the second support rods and the second springs is two groups. The two groups of the second support rods and the second springs are equidistantly arrayed on the outer wall of the guide plate with the center line of the third support plate as the rotation axis. The second support rods are used to guide and support through the guide plate when the angle offset generated by the deflection of the mouse's head occurs.

[0012] According to the above technical solution, the outer wall of the second support plate is slidably connected to the inner wall of the limiting plate, and the outer wall of the limiting rod is slidably connected to the wall of the first guide groove. The first guide groove is used to guide the limiting rod.

[0013] According to the above technical solution, the surface of the fifth support plate contacts the bottom of the first support plate, and the outer wall of the fourth support plate is fixedly connected to the output end of the second cylinder. The second cylinder is used to drive the fourth support plate to adjust the position of the adjusting plate for supporting the mouse's head. A three-dimensional positioning automatic cerebrospinal fluid extraction system for experimental mice includes a central control system, a positioning mechanism, an adjustment mechanism, and an extraction tube. The central control system controls the positioning mechanism and the adjustment mechanism respectively, and receives the position information of the positioning mechanism and the position information of the adjustment mechanism. The central control system controls the position information of the extraction tube and monitors the working state of the extraction tube.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The three-dimensional positioning automatic cerebrospinal fluid extraction device for experimental mice positions the mouse through the positioning mechanism. After adjusting the body position of the mouse, the head of the mouse is clamped to fix the head of the mouse, increasing the stability of the cerebrospinal fluid extraction process, reducing the operation difficulty, and enabling the extraction device to extract cerebrospinal fluid from the mouse more stably.

[0015] 2. The three-dimensional positioning automatic cerebrospinal fluid extraction device for experimental mice adjusts the position of the mouse's head through the adjustment mechanism, tilting the mouse's head downward to make the cerebrospinal fluid converge by natural gravity, increasing the quality of the extracted sample, and improving the working efficiency of the extraction device for extracting cerebrospinal fluid from the mouse.

[0016] 3. The three-dimensional positioning automatic cerebrospinal fluid extraction device for experimental mice compensates for the horizontal displacement generated during the clamping process of the mouse's head through the adjustment block, preventing the clamping failure caused by the offset of the mouse's head, and enabling the extraction device to stably clamp the mouse's head.

[0017] 4. The three-dimensional positioning automatic cerebrospinal fluid extraction device for experimental mice compensates for the deflection angle generated during the deflection adjustment of the mouse's head by the third support plate, ensuring stable clamping when adjusting the tilting angle of the mouse's head, and enabling the extraction device to stably and accurately extract cerebrospinal fluid from the mouse. Description of the Drawings

[0018] Figure 1 Structural Schematic of the Present InventionFigure 1 ; Figure 2 is a cross-section of the present invention Figure 1 ; Figure 3 is a schematic structural diagram of the present invention Figure 2 ; Figure 4 is a cross-section of the present invention Figure 2 ; Figure 5 is a schematic structural diagram of the positioning mechanism of the present invention; Figure 6 is a cross-section of the positioning mechanism of the present invention Figure 1 ; Figure 7 is a cross-section of the positioning mechanism of the present invention Figure 2 ; Figure 8 is a schematic structural diagram of the adjustment mechanism of the present invention; Figure 9 is a schematic diagram of the framework of the system of the present invention.

[0019] In the figure: 1, support base; 101, first support plate; 102, support frame; 103, first cylinder; 104, extraction pipe; 105, limit plate; 106, second cylinder; 107, guide rod; 108, first sliding groove; 109, first guide groove; 2, positioning mechanism; 201, limit rod; 202, second support plate; 203, first spring; 204, third cylinder; 205, third support plate; 206, second guide groove; 207, second spring; 208, guide plate; 209, second sliding groove; 210, adjustment block; 211, guide sleeve; 212, first support rod; 213, support block; 214, positioning block; 215, clamping jaw; 216, third sliding groove; 217, third spring; 218, second support rod; 3, adjustment mechanism; 301, fourth support plate; 302, fourth cylinder; 303, adjustment plate; 304, fifth support plate; 305, fourth spring. Specific embodiments

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Example 1, please refer to Figures 1 - 7, the present invention provides a technical solution: a three-dimensional positioning automatic cerebrospinal fluid extraction device for experimental mice, including a support base 1, a support frame 102 is fixedly connected to the top of the support base 1, a cylinder 103 is fixedly connected to the outer wall of the support frame 102, the output end of the cylinder 103 penetrates through the outer wall of the support frame 102 and is fixedly connected to an extraction tube 104, a cylinder 106 is fixedly connected to the inner wall of the support base 1, a first support plate 101 is slidably connected to the inner wall of the support base 1, a guide rod 107 is fixedly connected to the inner wall of the support base 1, a limit plate 105 is fixedly connected to the outer wall of the support base 1, a first guide groove 109 is opened on the inner wall of the limit plate 105, a first sliding groove 108 is opened on the outer wall of the support base 1, an adjustment mechanism 3 is arranged inside the support base 1, and further includes: Positioning mechanism 2 is arranged inside the support base 1. The positioning mechanism 2 includes a positioning block 214. A first support rod 212 is fixedly connected to the outer wall of the positioning block 214. A guide sleeve 211 is slidably connected to the outer wall of the first support rod 212. An adjustment block 210 is fixedly connected to the outer wall of the guide sleeve 211. The inner wall of the adjustment block 210 is slidably connected to the wall of a second sliding groove 209 formed in the outer wall of a guide plate 208 through a slider. A second support rod 218 is fixedly connected to the outer wall of the guide plate 208. The outer wall of the second support rod 218 is slidably connected to the wall of a second guide groove 206 formed in the outer wall of a third support plate 205. A support block 213 is fixedly connected to the outer wall of the positioning block 214. The outer wall of the support block 213 is slidably connected to the wall of a third sliding groove 216 formed in the outer wall of a clamping jaw 215 through a slider. The first support rod 212 is used to position the head of a mouse, and the clamping jaw 215 is used to fixedly clamp the head of the mouse. When the stereotactic experimental rodent cerebrospinal fluid automatic extraction device is put into use, pull out the first support plate 101, place the anesthetized mouse on the surface of the first support plate 101, adjust the posture of the mouse to the prone position, and after placement, push in the first support plate 101 to align the head of the mouse with the positioning mechanism 2, position and clamp the head of the mouse. Drive the positioning block 214 to contact the head of the mouse by starting the third cylinder 204, and support the first support rod 212 through a third spring 217 to adjust the position of the head of the mouse. After the adjustment is completed, the head of the mouse drives the support block 213 to slide on the inner wall of the third sliding groove 216 through the positioning block 214. Support the clamping jaw 215 through the hinge connection between the adjustment block 210 and the clamping jaw 215, so that the support block 213 drives the clamping jaw 215 to swing towards the head of the mouse during the sliding process on the inner wall of the third sliding groove 216 to clamp the head of the mouse. After the head of the mouse is clamped, start the second cylinder 106 to drive the adjustment mechanism 3 to align with the head of the mouse, retract the first support plate 101 to expose the head of the mouse, and support it through the adjustment mechanism 3. Adjust the tilting direction of the head of the mouse through the adjustment mechanism. During the adjustment process, the second support rod 218 compresses the second spring 207 and slides on the inner wall of the second guide groove 206 to compensate for the angular deviation generated by the head of the mouse. At the same time, the adjustment block 210 slides on the inner wall of the second sliding groove 209 to compensate for the horizontal deviation generated by the head of the mouse. The second support plate 202 compresses the first spring 203 to drive the limit rod 201 to slide on the inner wall of the first guide groove 109 to compensate for the deviation in the numerical direction generated by the head of the mouse. After the adjustment mechanism 3 completes the adjustment of the head of the mouse, start the first cylinder 103 to drive the extraction tube 104 to extract the cerebrospinal fluid of the mouse. After the extraction is completed and the first cylinder 103 drives the extraction tube 104 to retract, the second cylinder 106 drives the adjustment mechanism 3 to retract. The third cylinder 204 drives the clamping jaw 215 and the positioning block 214 to release the clamping of the head of the mouse, pull out the first support plate 101, remove the mouse after the cerebrospinal fluid extraction is completed, and wait for the next extraction operation; A third spring 217 is fixedly connected to the outer wall of the first support rod 212, and the other end of the third spring 217 is fixedly connected to the inner wall of the adjustment block 210. A second spring 207 is fixedly connected to the wall of the second guide groove 206, and the other end of the second spring 207 is fixedly connected to the outer wall of the second support rod 218. The second guide groove 206 is used to guide the second support rod 218. During the clamping of the mouse's head, the first support rod 212 is driven by the third spring 217 to squeeze the positioning block 214 to perform positioning adjustment on the mouse's head, so that the mouse can be stably clamped. When the adjusting mechanism 3 makes an inclination adjustment to the mouse's head, the second support rod 218 compresses the second spring 207 and slides on the inner wall of the second guide groove 206, so that the guide plate 208 deflects synchronously with the mouse's head to compensate for the angular offset generated by the mouse's head; The outer wall of the third support plate 205 is fixedly connected to the output pipe of the third cylinder 204 through the first sliding groove 108. The bottom of the third cylinder 204 is fixedly connected to the second support plate 202. The bottom of the second support plate 202 is fixedly connected to the limiting rod 201. The bottom of the second support plate 202 is fixedly connected to the first spring 203. The other end of the first spring 203 is fixedly connected to the inner wall of the limiting plate 105. The limiting rod 201 is used to support the vertical positioning of the mouse's head and compensate for the vertical displacement generated after the mouse's head is clamped. After the mouse's head is clamped, during the process of adjusting the inclination angle of the mouse's head by the adjusting mechanism 3, the third cylinder 204 is supported by the second support plate 202, so that the positioning block 214 and the clamping jaw 215 maintain a stable clamping of the mouse. At the same time, for the vertical offset generated by the mouse's head, the second support plate 202 compresses the first spring 203 to drive the limiting rod 201 to slide on the inner wall of the first guide groove 109 to compensate for the vertical offset generated by the adjustment of the mouse's head and keep the mouse's body posture stable; The outer wall of the adjustment block 210 is hinged to the outer wall of the clamping jaw 215 through a rotating shaft. The number of the second support rods 218 is two groups, and the two groups of second support rods 218 are symmetrically arranged on the outer wall of the guide plate 208 with the center line of the guide plate 208 as the axis of symmetry. The number of the clamping jaws 215 is two groups, and the two groups of clamping jaws 215 are symmetrically arranged on the outer wall of the adjustment block 210 with the center line of the adjustment block 210 as the axis of symmetry. The adjustment block 210 is used to compensate for the horizontal displacement generated after the mouse's head is clamped and support the clamping jaw 215 after the mouse's head is fixedly clamped. By starting the third cylinder 204, the positioning block 214 is driven to contact the mouse's head, and the first support rod 212 is supported by the third spring 217 to perform positioning adjustment on the mouse's head. After the adjustment is completed, the mouse's head drives the support block 213 to slide on the inner wall of the third sliding groove 216 through the positioning block 214. The adjustment block 210 is hinged to the clamping jaw 215 to support the clamping jaw 215, so that the support block 213 drives the clamping jaw 215 to swing towards the mouse's head during the sliding process on the inner wall of the third sliding groove 216 to clamp the mouse's head; There are two sets of both the second support rod 218 and the second spring 207. The two sets of the second support rod 218 and the second spring 207 are equidistantly arrayed on the outer wall of the guide plate 208 with the center line of the third support plate 205 as the rotation axis. The second support rod 218 is used to guide and support through the guide plate 208 when the angular offset generated by the deflection of the mouse's head. When the adjusting mechanism 3 tilts the mouse's head, the second support rod 218 compresses the second spring 207 and slides on the inner wall of the second guide groove 206, so that the guide plate 208 deflects synchronously with the mouse's head to compensate for the angular offset generated by the mouse's head. The outer wall of the second support plate 202 is slidably connected to the inner wall of the limiting plate 105, and the outer wall of the limiting rod 201 is slidably connected to the groove wall of the first guide groove 109. The first guide groove 109 is used to guide the limiting rod 201. The cylinder three 204 is supported by the second support plate 202, so that the positioning block 214 and the clamping jaw 215 maintain stable clamping of the mouse. At the same time, the numerical direction offset generated by the mouse's head compresses the first spring 203 through the second support plate 202 to drive the limiting rod 201 to slide on the inner wall of the first guide groove 109 to compensate for the vertical offset generated by the adjustment of the mouse's head.

[0022] Embodiment 2, based on Embodiment 1, please refer to Figure 8 , the present invention provides a technical solution: The adjusting mechanism 3 includes a fourth support plate 301. The inner wall of the fourth support plate 301 is slidably connected to the outer wall of the guide rod 107. The outer wall of the fourth support plate 301 is fixedly connected to a fourth cylinder 302. The output end of the fourth cylinder 302 penetrates the outer wall of the fourth support plate 301 and is fixedly connected to the outer wall of the adjusting plate 303. A fifth support plate 304 is slidably connected to the inner wall of the fourth support plate 301. The bottom of the fifth support plate 304 is fixedly connected to a fourth spring 305. The other end of the fourth spring 305 is fixedly connected to the inner wall of the fourth support plate 301. The adjusting plate 303 is used to deflect the mouse's head, and the fifth support plate 304 is used to support the mouse's head. After the mouse's head is clamped, the second cylinder 106 drives the fourth support plate 301 to slide on the outer wall of the guide rod 107, so that the adjusting plate 303 is aligned with the mouse's head. The fourth cylinder 302 is started to drive the adjusting plate 303 to contact the mouse's head to adjust the tilt angle of the mouse's head. When the mouse's head tilts downward, the mouse's head is supported by the fifth support plate 304. The fifth support plate 304 compresses the fourth spring 305 and slides on the inner wall of the fourth support plate 301, so that the mouse's head tilts at an angle of 30°, exposing the foramen magnum of the mouse and aligning it with the extraction tube 104. After the mouse's head is adjusted, the first cylinder 103 is started to drive the extraction tube 104 to extract the cerebrospinal fluid of the mouse; The surface of the fifth support plate 304 is in contact with the bottom of the first support plate 101. The outer wall of the fourth support plate 301 is fixedly connected to the output end of the second cylinder 106. The second cylinder 106 is used to drive the fourth support plate 301 to adjust the position of the adjustment plate 303 in supporting the head of the mouse. After the head of the mouse is clamped, the second cylinder 106 is started to drive the fourth support plate 301 to slide on the outer wall of the guide rod 107, so that the position of the adjustment plate 303 is aligned with the head of the mouse. The first support plate 101 is retracted to expose the fifth support plate 304 to support the head of the mouse, and the tilt direction of the head of the mouse is adjusted.

[0023] Embodiment 3. Based on Embodiment 1 and Embodiment 2, please refer to Figure 9 , the present invention provides a technical solution: a three-dimensional positioning automatic cerebrospinal fluid extraction system for experimental mice, including a central control system, a positioning mechanism, an adjustment mechanism, and an extraction tube. The central control system controls the positioning mechanism and the adjustment mechanism respectively, and receives the position information of the positioning mechanism and the position information of the adjustment mechanism. The central control system controls the position information of the extraction tube and monitors the working state of the extraction tube. When the positioning mechanism detects that the head of the mouse is aligned with the positioning mechanism, the positioning mechanism feeds back the information to the central control system. The central control system issues a signal to make the positioning mechanism position and clamp the head of the mouse. After the clamping is completed and the positioning mechanism feeds back the information, the central control system continues to issue an instruction to make the adjustment mechanism adjust the tilt angle of the head of the mouse. After the adjustment is completed, the adjustment mechanism feeds back the information to the central control system. The central control system issues an instruction to the extraction tube to extract the cerebrospinal fluid of the mouse. After the extraction is completed, the extraction tube feeds back the information to the central control system. The central control system issues an instruction to the positioning mechanism and the adjustment mechanism to release the clamping, so that the positioning mechanism and the adjustment mechanism retract.

[0024] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for automatically extracting cerebrospinal fluid from mice in a stereotaxic experimental setting, comprising a support seat (1), the top of the support seat (1) being fixedly connected to a support frame (102), the outer wall of the support frame (102) being fixedly connected to a cylinder 1 (103), the output end of the cylinder 1 (103) passing through the outer wall of the support frame (102) being fixedly connected to an extraction tube (104), the inner wall of the support seat (1) being fixedly connected to a cylinder 2 (106), the inner wall of the support seat (1) being slidably connected to a support plate 1 (101), and the inner wall of the support seat (1) being fixedly connected to a guide rod (107), characterized in that: The outer wall of the support seat (1) is fixedly connected to a limit plate (105), the inner wall of the limit plate (105) is provided with a guide groove (109), the outer wall of the support seat (1) is provided with a sliding groove (108), and an adjustment mechanism (3) is arranged inside the support seat (1), and further comprises: A positioning mechanism (2), the positioning mechanism (2) being arranged inside the support seat (1), the positioning mechanism (2) comprising a positioning block (214), the outer wall of the positioning block (214) being fixedly connected to a support rod 1 (212), the outer wall of the support rod 1 (212) being slidably connected to a guide sleeve (211), the outer wall of the guide sleeve (211) being fixedly connected to an adjustment block (210), the inner wall of the adjustment block (210) being slidably connected to a groove wall of a sliding groove 2 (209) provided on the outer wall of a guide plate (208) via a sliding block, the guide plate ( The outer wall of the support plate (208) is fixedly connected to a support rod 2 (218), and the outer wall of the support rod 2 (218) is slidably connected to the groove wall of the guide groove 2 (206) opened on the outer wall of the support plate 3 (205). The outer wall of the positioning block (214) is fixedly connected to a support block (213), and the outer wall of the support block (213) is slidably connected to the groove wall of the sliding groove 3 (216) opened on the outer wall of the clamp (215) through a slider. The support rod 1 (212) is used to position the mouse head, and the clamp (215) is used to fix and clamp the mouse head.

2. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 1, characterized in that: The outer wall of the support rod one (212) is fixedly connected with a spring three (217), the other end of the spring three (217) is fixedly connected to the inner wall of the adjustment block (210), the groove wall of the guide groove two (206) is fixedly connected with a spring two (207), the other end of the spring two (207) is fixedly connected to the outer wall of the support rod two (218), and the guide groove two (206) is used to guide the support rod two (218).

3. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 1, characterized in that: The outer wall of the support plate three (205) is fixedly connected to the output pipe of the cylinder three (204) through the sliding groove one (108); the bottom of the cylinder three (204) is fixedly connected to the support plate two (202); the bottom of the support plate two (202) is fixedly connected to a limiting rod (201); the bottom of the support plate two (202) is fixedly connected to a spring one (203); the other end of the spring one (203) is fixedly connected to the inner wall of the limiting plate (105); the limiting rod (201) is used to support the vertical positioning of the mouse head and compensate for the vertical displacement generated after the mouse head is clamped.

4. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 1, characterized in that: The adjustment mechanism (3) comprises a support plate four (301), the inner wall of the support plate four (301) is slidably connected to the outer wall of the guide rod (107), the outer wall of the support plate four (301) is fixedly connected to a cylinder four (302), the output end of the cylinder four (302) passes through the outer wall of the support plate four (301) and is fixedly connected to the outer wall of the adjustment plate (303), the inner wall of the support plate four (301) is slidably connected to a support plate five (304), the bottom of the support plate five (304) is fixedly connected to a spring four (305), the other end of the spring four (305) is fixedly connected to the inner wall of the support plate four (301), the adjustment plate (303) is used to deflect the mouse head, and the support plate five (304) is used to support the mouse head.

5. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 1, characterized in that: The outer wall of the adjustment block (210) is hingedly connected to the outer wall of the clamping claw (215) via a rotating shaft. The number of the second support rods (218) is two groups, and the two groups of the second support rods (218) are symmetrically arranged on the outer wall of the guide plate (208) with the center line of the guide plate (208) as the symmetry axis. The number of the clamping claws (215) is two groups, and the two groups of the clamping claws (215) are symmetrically arranged on the outer wall of the adjustment block (210) with the center line of the adjustment block (210) as the symmetry axis. The adjustment block (210) is used to compensate for the horizontal displacement generated after clamping the mouse head, and to support the clamping claws (215) after fixing and clamping the mouse head.

6. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 2, characterized in that: The number of the second support rod (218) and the second spring (207) is two groups, and the two groups of the second support rod (218) and the second spring (207) are arranged equidistantly on the outer wall of the guide plate (208) with the center line of the third support plate (205) as the rotation axis. The second support rod (218) is used to guide and support the mouse head through the guide plate (208) when the angle is offset due to the deflection.

7. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 3, characterized in that: The outer wall of the second support plate (202) is slidably connected to the inner wall of the limiting plate (105), and the outer wall of the limiting rod (201) is slidably connected to the groove wall of the first guide groove (109), and the first guide groove (109) is used to guide the limiting rod (201).

8. The automatic extraction device for cerebrospinal fluid of mice in stereotaxic experiments according to claim 4, characterized in that: The surface of the support plate five (304) contacts the bottom of the support plate one (101), and the outer wall of the support plate four (301) is fixedly connected to the output end of the cylinder two (106). The cylinder two (106) is used to drive the support plate four (301) to adjust the position of the adjustment plate (303) on the mouse head support.

9. A stereotactic experimental rat cerebrospinal fluid automatic extraction system, comprising a central control system, a positioning mechanism, an adjustment mechanism, and an extraction tube, characterized in that: The central control system controls the positioning mechanism and the adjusting mechanism respectively, and collects the position information of the positioning mechanism and the position information of the adjusting mechanism. The central control system controls the position information of the extraction pipe and monitors the working state of the extraction pipe.

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