Device and method for concentrating articular cartilage regenerated collagen solution

Abstract

The invention discloses a device and method for concentrating an articular cartilage regenerated collagen solution, and relates to the technical field of collagen concentrating devices.The device comprises a concentrating tank, an ultrafiltration membrane separator, a vacuum pump and an electromagnetic stirrer are installed on the top side of the concentrating tank, and a heating coil is installed on an interlayer of an inner tank body and an outer tank body of the concentrating tank; a heating coil is arranged in the concentration tank, an electric heating wire is arranged in the heating coil, a rotor is arranged on the inner wall of the top side of the concentration tank, the rotor is coupled with an electromagnetic stirrer, and a ventilation mechanism used for heating and introducing inert gas and disturbing water flow in the heating coil is mounted on the heating coil. The heating inert gas is in direct contact with the collagen solution, uniform heating is achieved, collagen denaturation is prevented, the viscosity of the collagen solution is reduced, the gas drum membrane is continuously shrunk and expanded through the intermittently introduced inert gas, water flow in the heating coil pipe is disturbed, uniform heating is facilitated, and scaling is prevented at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen concentration devices, and particularly to a device and a method for concentrating a collagen solution for articular cartilage regeneration. Background Art

[0002] With the development of medical treatment, it has been found that collagen can induce human stem cells to generate new cartilage, and thus it is widely used in articular cartilage regeneration. Although collagen widely exists in the bodies of vertebrates, filtering and concentration are still required to produce medical-grade materials from this natural collagen. The patent with the Chinese patent publication number CN105289021B discloses a device and a method for concentrating a collagen solution for articular cartilage regeneration, including a sealed housing, a plurality of trays for accommodating the collagen solution, and a motor. The motor has a motor shaft that is substantially perpendicular to the trays. The trays are arranged in the housing and each of them includes an outer wall and a bottom surface. The bottom surfaces of the trays are arranged substantially parallel to each other. In addition, the device further includes a plurality of stirring devices driven by the motor shaft. One stirring device is provided above each tray. Each stirring device includes a plurality of blades for stirring the collagen solution, which has a reduced cost and improved efficiency in manufacturing collagen. The produced collagen is particularly suitable for articular cartilage regeneration.

[0003] In the prior art, when concentrating the collagen solution, after the air in the concentration tank is evacuated by a vacuum pump, the boiling point of the water in the collagen solution is reduced. Then, the concentration tank is heated by a heating coil through a controller, and at the same time, the collagen solution is stirred by an electromagnetic stirrer to evaporate the water. The ultrafiltration membrane separator is started to absorb and discharge the evaporated water and small molecules, and the collagen flows back and re-enters the concentration tank to complete the concentration process. However, in this process, due to the non-contact heating of the heating coil, there is a risk of uneven heating, which may cause the collagen to denature. Moreover, the viscosity of the collagen increases during the concentration process, resulting in poor concentration effect. For this reason, we propose a device and a method for concentrating a collagen solution for articular cartilage regeneration. Summary of the Invention

[0004] The purpose of the present invention is to provide a device and a method for concentrating a collagen solution for articular cartilage regeneration, so as to solve the problems raised in the above background art.

[0005] To achieve the above object, the present invention provides the following technical solution: A device for concentrating a collagen solution for articular cartilage regeneration, comprising a concentration tank. An ultrafiltration membrane separator, a vacuum pump, and an electromagnetic stirrer are installed on the top side of the concentration tank. A heating coil is installed on the interlayer between the inner and outer tanks of the concentration tank, and an electric heating wire is arranged inside the heating coil. A rotor is arranged on the inner wall of the top side of the concentration tank, and the rotor is coupled with the electromagnetic stirrer. An air venting mechanism for heating the inert gas introduced and disturbing the water flow inside the heating coil is installed on the heating coil. A connecting wall pipe is installed on the top side of the concentration tank, and the connecting wall pipe is in communication with the air venting mechanism. A rotating mechanism for intermittently introducing inert gas is installed on the rotor, and the rotating mechanism is located on one side of the connecting wall pipe. A stirring hollow pipe is installed on the rotor, and the stirring hollow pipe is in communication with the rotating mechanism. An aeration mechanism for uniformly introducing inert gas is installed on the stirring hollow pipe. A stirring mechanism for stirring the collagen solution is installed on the stirring hollow pipe. A filtering mechanism is installed on the air venting mechanism. When concentrating the collagen solution, after the air in the concentration tank is evacuated by the vacuum pump, the boiling point of the water in the collagen solution is reduced. Then, the concentration tank is heated by the controller through the heating coil, and at the same time, the collagen solution is stirred by the electromagnetic stirrer to evaporate the water. The ultrafiltration membrane separator is started to absorb and discharge the evaporated water and small molecules, and the collagen flows back and re-enters the concentration tank to complete the concentration process. During this process, inert gas is introduced into the air venting mechanism, so that when the controller heats the water in the heating coil through the electric heating wire, the passing inert gas is heated. The heated inert gas is intermittently introduced into the bottom side of the concentration tank through the aeration mechanism during the movement of the rotating mechanism, which is beneficial to uniformly heating the temperature of the collagen solution, reducing the denaturation of collagen caused by the temperature gradient. At the same time, micro-turbulence will be generated during the upward movement of the inert gas in the concentration tank, reducing the concentration polarization, and can briefly break the hydrogen bond network formed between collagen molecules, reducing the local viscosity, which is beneficial to the concentration process. The stirring mechanism provided can also eliminate the generated bubbles during the stirring process of the collagen solution, improving the practicability and scientificity of the device. In addition, the filtering mechanism filters the incoming inert gas to prevent contamination of the collagen solution, further improving the scientificity of the structure.

[0006] Further, the ventilation mechanism includes an intake pipe installed on the heating coil. An air coil is installed on the intake pipe. The air coil is located between the heating coil and the heating wire, and on the side of the heating wire closer to the concentration tank. An installation block is installed on the air coil and on the inner wall of the heating coil. An air diaphragm is installed on the side of the air coil closer to the concentration tank. An outlet pipe is installed on the air coil and on the connecting wall pipe. After the inert gas enters the intake pipe, it bypasses through the air coil and then enters the connecting wall pipe through the outlet pipe. During this process, the heating wire heats the water in the heating coil and simultaneously heats the passing inert gas. Since the inert gas is intermittently introduced into the concentration tank, when the inert gas is continuously introduced but not into the concentration tank, it drives the air diaphragm to bulge. According to the above principle, when the inert gas is introduced into the concentration tank, the air diaphragm contracts. Thus, during the process of intermittently introducing the inert gas into the concentration tank, the air diaphragm repeats the process of bulging and contracting, thereby realizing the disturbance of the water in the heating coil, preventing the generation of local temperature gradients, facilitating the uniform heating of the concentration tank, and preventing scaling on the inner wall of the heating coil closer to the concentration tank during this process, further facilitating the heating process.

[0007] Further, the rotation mechanism includes a through pipe installed on the rotor. The through pipe communicates with the stirring hollow pipe. A ball valve block is installed on the through pipe. An adapted ball groove is provided on the concentration tank. The ball valve block is rotatably installed in the adapted ball groove. An L-shaped bent hole is provided on the ball valve block and communicates with the connecting wall pipe. When the electromagnetic stirrer drives the rotor to rotate, the rotor drives the ball valve block to rotate in the adapted ball groove. When the L-shaped bent hole on the ball valve block communicates with the connecting wall pipe, the inert gas is introduced into the stirring hollow pipe. According to the above principle, when the L-shaped bent hole on the ball valve block is misaligned with the connecting wall pipe, the inert gas cannot be introduced, thereby realizing the intermittent introduction of the inert gas.

[0008] Further, the aeration mechanism includes an aeration chamber installed on the stirring hollow pipe. Air holes are provided on the aeration chamber. A sliding shaft block is installed on the aeration chamber. A piston disk is slidably installed on the sliding shaft block. The lower part of the piston disk contacts the inner wall of the aeration chamber, and the upper part of the piston disk contacts the inner wall of the stirring hollow pipe. A spring is movably sleeved on the sliding shaft block and installed on the piston disk. When the inert gas is introduced into the stirring hollow pipe, it drives the piston disk to slide on the sliding shaft block and exposes the air holes, so that the inert gas is evenly introduced into the bottom of the concentration tank through the air holes. And when the inert gas is not introduced, the piston disk is driven to reset under the restoring elastic force of the spring, preventing the backflow of the collagen solution, further improving the scientificity and practicality of the structure.

[0009] Further, the stirring mechanism includes a cross bar installed on the stirring hollow tube. Stirring blades are installed on the cross bar. The stirring blades are symmetrically arranged and do not contact each other. A top plate is installed on the stirring hollow tube. A limiting slide bar is slidably installed on the top plate. An adaptive floating plate is installed on the limiting slide bar. An arc wall is installed on the adaptive floating plate. The arc wall contacts the concentration tank. During the rotation of the stirring hollow tube, the stirring blades are driven to rotate to stir the collagen solution. And as the concentration process proceeds, the liquid level of the collagen decreases. Through the buoyancy of the adaptive floating plate, it is convenient to still rotate and remove the bubbles on the liquid surface as the liquid level drops. And during this process, the inner wall of the concentration tank is scraped by the arc wall to prevent collagen residue. It should be noted that the stirring blades are composed of helical blades arranged symmetrically. The adaptive floating plate can pass through the gap between the stirring blades as the liquid level drops.

[0010] Further, the filtering mechanism includes a filter chamber installed on the intake pipe. A main rotating roller is rotatably installed on the filter chamber. A filter membrane belt is movably sleeved on the main rotating roller. A driven roller is movably sleeved on the filter membrane belt. The driven roller is rotatably installed on the filter chamber. The intake pipe contacts the filter membrane belt. When the main rotating roller rotates, it drives the filter membrane belt to rotate on the driven roller and the main rotating roller, thereby realizing real-time replacement of the contact surface between the filter membrane belt and the intake pipe and improving the filtering effect.

[0011] Further, a motor is installed on the filter chamber. The main shaft of the motor is installed on the main rotating roller. Starting the motor enables the motor to drive the main rotating roller to rotate.

[0012] Further, a gear is installed on the main rotating roller. A gear column is rotatably installed on the filter chamber. The gear column meshes with the gear. A sliding disk is slidably installed on the gear column. A connecting shaft is installed on the sliding disk. A membrane brush is installed on the connecting shaft. The membrane brush contacts the filter membrane belt. When the main rotating roller rotates, it drives the gear to rotate. The rotation of the gear drives the gear column to rotate. The rotation of the gear column drives the sliding disk to slide reciprocally. The reciprocal sliding of the sliding disk drives the connecting shaft to slide reciprocally. The reciprocal sliding of the connecting shaft drives the membrane brush to slide reciprocally on the surface of the filter membrane belt to scrape off the sundries attached to the surface of the filter membrane belt.

[0013] Further, an elliptical wall groove is formed on the gear column. A transmission shaft block is installed on the sliding disk. The transmission shaft block is slidably installed in the elliptical wall groove. Through the cooperation of the elliptical wall groove and the transmission shaft block, it is realized that when the gear column rotates, it drives the transmission shaft block to slide reciprocally along the elliptical wall groove, thereby driving the sliding disk to slide reciprocally.

[0014] A method for concentrating a collagen solution for articular cartilage regeneration includes the following steps: S1: The controller is started to heat the heating coil, and the temperature is maintained at 35 - 40 degrees Celsius. Meanwhile, the inert gas is introduced along the inlet pipe, and the filtering mechanism is started to filter the passing inert gas. When the inert gas initially enters the concentration tank, the connecting wall pipe is closed by the rotation of the ball valve block, and the introduction of the inert gas is stopped. S2: The vacuum pump is started to extract the air in the concentration tank, so that the air pressure in the concentration tank is lower than -0.094 Mpa. S3: The electromagnetic stirrer is started and the inert gas is continuously introduced again. The electromagnetic stirrer drives the rotor to rotate, the rotation of the rotor drives the ball valve block to rotate, the rotation of the rotor drives the stirring blades and the matching floating plate to rotate. The ultrafiltration membrane separator is started to absorb small molecules such as evaporated water, and the collagen flows back into the concentration tank.

[0015] Compared with the prior art, the beneficial effects of the present invention are: In the present invention, through the settings of the air coil, air drum membrane, ball valve block, aeration chamber, etc., when concentrating the collagen solution, the inert gas is intermittently introduced into the concentration tank after being heated by the air coil, so as to use the inert gas as an indirect heat source to directly contact the collagen solution, realizing uniform heating, preventing collagen denaturation, and the introduced inert gas briefly breaks the hydrogen bond network formed when the collagen becomes concentrated during the floating process, thereby reducing the viscosity of the collagen solution and being beneficial to the concentration process. In addition, the intermittently introduced inert gas causes the air drum membrane to continuously contract and expand, disturbing the water flow in the heating coil, which is beneficial to uniform heating and prevents scale formation.

[0016] In the present invention, through the settings of the matching floating plate, arc-shaped wall, etc., during the concentration process, the bubbles generated on the liquid surface are continuously removed as the liquid level changes, which is beneficial to the collagen concentration process.

[0017] In the present invention, through the settings of the sliding shaft block, piston disk, spring, etc., when the inert gas is introduced into the stirring hollow tube, it drives the piston disk to slide on the sliding shaft block and exposes the air holes, so that the inert gas is uniformly introduced into the bottom of the concentration tank through the air holes, and when the inert gas is not introduced, it drives the piston disk to reset under the restoring elastic force of the spring, preventing the collagen solution from flowing back, and further improving the scientificity and practicality of the structure.

[0018] In the present invention, through the settings of the filter membrane belt, main rotating roller, driven roller, etc., when the main rotating roller rotates, it drives the filter membrane belt to rotate on the driven roller and the main rotating roller, thereby realizing the real-time replacement of the contact surface between the filter membrane belt and the inlet pipe, improving the filtering effect. Brief Description of the Drawings

[0019] Figure 1 is the overall structural schematic diagram of the present invention; Figure 2 is the structural schematic diagram of the heating coil of the present invention; Figure 3 The enlarged structural schematic diagram of part A in the present invention Figure 2 ; Figure 4 The partial sectional structural schematic diagram of the heating coil in the present invention Figure 5 The sectional structural schematic diagram of the stirring hollow tube of the water tank Figure 6 The structural schematic diagram of the fitting ball groove in the present invention Figure 7 The partial sectional structural schematic diagram of the concentration tank in the present invention Figure 8 The structural schematic diagram of the stirring blade in the present invention Figure 9 The sectional schematic diagram of the aeration chamber in the present invention Figure 10 The internal structural schematic diagram of the filter chamber in the present invention Figure 11 The partial sectional structural schematic diagram of the main rotating roller in the present invention

[0020] In the figure: 1, concentration tank; 2, ultrafiltration membrane separator; 3, vacuum pump; 4, electromagnetic stirrer; 5, controller; 6, heating coil; 7, heating wire; 8, rotor; 9, ventilation mechanism; 901, intake pipe; 902, air coil; 903, mounting block; 904, air diaphragm; 905, outlet pipe; 10, connecting wall pipe; 11, rotating mechanism; 1101, through pipe; 1102, ball valve block; 1103, L-shaped bent hole; 1104, fitting ball groove; 12, stirring hollow tube; 13, stirring mechanism; 1301, cross bar; 1302, stirring blade; 1303, top plate; 1304, limiting slide bar; 1305, fitting floating plate; 1306, arc wall; 14, aeration mechanism; 1401, aeration chamber; 1402, air hole; 1403, sliding shaft block; 1404, piston disc; 1405, spring; 15, filtering mechanism; 1501, filter chamber; 1502, main rotating roller; 1503, filter membrane belt; 1504, driven roller; 1505, motor; 1506, gear; 1507, gear column; 1508, sliding disc; 1509, connecting shaft; 1510, membrane brush; 1511, drive shaft block; 1512, elliptical wall groove. Detailed implementation manners

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0022] Please refer toFigures 1-11 , a device for concentrating a collagen solution for articular cartilage regeneration in the illustration, includes a concentration tank 1. An ultrafiltration membrane separator 2, a vacuum pump 3, and an electromagnetic stirrer 4 are installed on the top side of the concentration tank 1. A heating coil 6 is installed on the interlayer between the inner and outer tanks of the concentration tank 1, and an electric heating wire 7 is arranged inside the heating coil 6. A rotor 8 is arranged on the inner wall of the top side of the concentration tank 1, and the rotor 8 is coupled with the electromagnetic stirrer 4. An air inlet mechanism 9 for heating and disturbing the water flow inside the heating coil 6 by introducing an inert gas is installed on the heating coil 6. A connecting wall pipe 10 is installed on the top side of the concentration tank 1, and the connecting wall pipe 10 communicates with the air inlet mechanism 9. A rotating mechanism 11 for intermittently introducing an inert gas is installed on the rotor 8, and the rotating mechanism 11 is located on one side of the connecting wall pipe 10. A stirring hollow pipe 12 is installed on the rotor 8, and the stirring hollow pipe 12 communicates with the rotating mechanism 11. An aeration mechanism 14 for uniformly introducing an inert gas is installed on the stirring hollow pipe 12. A stirring mechanism 13 for stirring the collagen solution is installed on the stirring hollow pipe 12. A filtering mechanism 15 is installed on the air inlet mechanism 9. When concentrating the collagen solution, after the air in the concentration tank 1 is pumped away by the vacuum pump 3, the boiling point of the water in the collagen solution is reduced. Then, the concentration tank 1 is heated by the controller 5 through the heating coil 6, and at the same time, the collagen solution is stirred by the electromagnetic stirrer 4 to evaporate the water. The ultrafiltration membrane separator 2 is started to absorb and discharge the evaporated water and small molecules, and the collagen flows back and re-enters the concentration tank 1 to complete the concentration process. During this process, the inert gas is introduced into the air inlet mechanism 9, so that when the controller 5 heats the water in the heating coil 6 through the electric heating wire 7, the passing inert gas is heated. The heated inert gas is intermittently introduced into the bottom side of the concentration tank 1 through the aeration mechanism 14 during the movement of the rotating mechanism 11, which is beneficial to uniformly heating the temperature of the collagen solution, reducing the denaturation of collagen caused by the temperature gradient. At the same time, micro-turbulence will be generated during the upward movement of the inert gas in the concentration tank 1, reducing the concentration polarization, and can briefly break the hydrogen bond network formed between collagen molecules, reducing the local viscosity, which is beneficial to the concentration process. The generated bubbles can be eliminated during the stirring of the collagen solution by the arranged stirring mechanism 13, improving the practicability and scientificity of the device. In addition, the entering inert gas is filtered by the filtering mechanism 15 to prevent pollution of the collagen solution, further improving the scientificity of the structure.

[0023] Refer to Figures 1-11, in the embodiment of the present invention, the ventilation mechanism 9 includes an intake pipe 901, the intake pipe 901 is installed on the heating coil 6, an air coil 902 is installed on the intake pipe 901, the air coil 902 is located between the heating coil 6 and the heating wire 7, the air coil 902 is located on the side of the heating wire 7 close to the concentration tank 1, an installation block 903 is installed on the air coil 902, the installation block 903 is installed on the inner wall of the heating coil 6, an air diaphragm 904 is installed on the side of the air coil 902 close to the concentration tank 1, an outlet pipe 905 is installed on the air coil 902, the outlet pipe 905 is installed on the connecting wall pipe 10. After the inert gas enters the intake pipe 901, it bypasses through the air coil 902 and then enters the connecting wall pipe 10 through the outlet pipe 905. During this process, the heating wire 7 heats the water in the heating coil 6 and simultaneously heats the passing inert gas. Since the inert gas is intermittently introduced into the concentration tank 1, when the inert gas is continuously introduced but not into the concentration tank 1, it drives the air diaphragm 904 to bulge. According to the above principle, when the inert gas is introduced into the concentration tank 1, the air diaphragm 904 contracts. Thus, during the process of intermittently introducing the inert gas into the concentration tank 1, the air diaphragm 904 repeats the process of bulging and contracting, thereby realizing the disturbance of the water in the heating coil 6, preventing the generation of local temperature gradients, being beneficial to the uniform heating of the concentration tank 1, and preventing scaling on the inner wall of the heating coil 6 close to the concentration tank 1 during this process, further facilitating the heating process.

[0024] Refer to Figures 1-8 , in the embodiment of the present invention, the rotation mechanism 11 includes a through pipe 1101, the through pipe 1101 is installed on the rotor 8, the through pipe 1101 is in communication with the stirring hollow pipe 12, a ball valve block 1102 is installed on the through pipe 1101, an adapted ball groove 1104 is formed on the concentration tank 1, the ball valve block 1102 is rotatably installed in the adapted ball groove 1104, an L-shaped bent hole 1103 is formed on the ball valve block 1102, and the L-shaped bent hole 1103 is in communication with the connecting wall pipe 10. When the electromagnetic stirrer 4 drives the rotor 8 to rotate, the rotor 8 drives the ball valve block 1102 to rotate in the adapted ball groove 1104. When the L-shaped bent hole 1103 on the ball valve block 1102 is in communication with the connecting wall pipe 10, the inert gas is introduced into the stirring hollow pipe 12. According to the above principle, when the L-shaped bent hole 1103 on the ball valve block 1102 is misaligned with the connecting wall pipe 10, the inert gas cannot be introduced, thereby realizing the intermittent introduction of the inert gas.

[0025] Refer to Figures 1-9, in the embodiment of the present invention, the aeration mechanism 14 includes an aeration chamber 1401. The aeration chamber 1401 is installed on the stirring hollow tube 12. Air holes 1402 are formed in the aeration chamber 1401. A sliding shaft block 1403 is installed on the aeration chamber 1401. A piston disc 1404 is slidably installed on the sliding shaft block 1403. The lower part of the piston disc 1404 is in contact with the inner wall of the aeration chamber 1401, and the upper part of the piston disc 1404 is in contact with the inner wall of the stirring hollow tube 12. A spring 1405 is movably sleeved on the sliding shaft block 1403. The spring 1405 is installed on the piston disc 1404. When inert gas is introduced into the stirring hollow tube 12, it drives the piston disc 1404 to slide on the sliding shaft block 1403 and exposes the air holes 1402, so that the inert gas is uniformly introduced into the bottom of the concentration tank 1 through the air holes 1402. And when the inert gas is not introduced, the piston disc 1404 is driven to reset under the restoring elastic force of the spring 1405, preventing the backflow of the collagen solution, and further improving the scientificity and practicality of the structure.

[0026] Refer to Figures 1-8 , in the embodiment of the present invention, the stirring mechanism 13 includes a cross bar 1301. The cross bar 1301 is installed on the stirring hollow tube 12. Stirring blades 1302 are installed on the cross bar 1301. The stirring blades 1302 are symmetrically arranged and do not contact each other. A top plate 1303 is installed on the stirring hollow tube 12. A limiting slide bar 1304 is slidably installed on the top plate 1303. An adaptive floating plate 1305 is installed on the limiting slide bar 1304. An arc wall 1306 is installed on the adaptive floating plate 1305. The arc wall 1306 is in contact with the concentration tank 1. During the rotation of the stirring hollow tube 12, the stirring blades 1302 are driven to rotate to stir the collagen solution. And as the concentration process progresses, the liquid level of the collagen decreases. Due to the buoyancy of the adaptive floating plate 1305, it is convenient to rotate and remove the bubbles on the liquid surface even when the liquid level drops. And during this process, the inner wall of the concentration tank 1 is scraped by the arc wall 1306 to prevent the residue of collagen. It should be noted that the stirring blades 1302 are composed of symmetrically arranged spiral blades, and the adaptive floating plate 1305 can pass through the gap between the stirring blades 1302 as the liquid level drops.

[0027] Refer to Figures 1-11 , in the embodiment of the present invention, the filtering mechanism 15 includes a filtering chamber 1501. The filtering chamber 1501 is installed on the air inlet pipe 901. A main rotating roller 1502 is rotatably installed on the filtering chamber 1501. A filter membrane belt 1503 is movably sleeved on the main rotating roller 1502. A driven roller 1504 is movably sleeved on the filter membrane belt 1503. The driven roller 1504 is rotatably installed on the filtering chamber 1501. The air inlet pipe 901 is in contact with the filter membrane belt 1503. When the main rotating roller 1502 rotates, it drives the filter membrane belt 1503 to rotate on the driven roller 1504 and the main rotating roller 1502, so as to realize the real-time replacement of the contact surface between the filter membrane belt 1503 and the air inlet pipe 901, improving the filtering effect.

[0028] Reference Figures 1-11 In the embodiment of the present invention, a motor 1505 is installed on the filter chamber 1501, and the main shaft of the motor 1505 is installed on the main rotating roller 1502. Starting the motor 1505 enables the motor 1505 to drive the main rotating roller 1502 to rotate.

[0029] Reference Figures 1-11 In the embodiment of the present invention, a gear 1506 is installed on the main rotating roller 1502, and a gear column 1507 is rotatably installed on the filter chamber 1501. The gear column 1507 meshes with the gear 1506. A sliding disk 1508 is slidably installed on the gear column 1507. A connecting shaft 1509 is installed on the sliding disk 1508, and a membrane brush 1510 is installed on the connecting shaft 1509. The membrane brush 1510 contacts the filter membrane belt 1503. When the main rotating roller 1502 rotates, it drives the gear 1506 to rotate. The rotation of the gear 1506 drives the gear column 1507 to rotate. The rotation of the gear column 1507 drives the sliding disk 1508 to reciprocate. The reciprocating sliding of the sliding disk 1508 drives the connecting shaft 1509 to reciprocate. The reciprocating sliding of the connecting shaft 1509 drives the membrane brush 1510 to reciprocate on the surface of the filter membrane belt 1503, scraping off the sundries attached to the surface of the filter membrane belt 1503.

[0030] Reference Figures 1-11 In the embodiment of the present invention, an elliptical wall groove 1512 is formed on the gear column 1507. A transmission shaft block 1511 is installed on the sliding disk 1508, and the transmission shaft block 1511 is slidably installed in the elliptical wall groove 1512. Through the cooperation of the elliptical wall groove 1512 and the transmission shaft block 1511, when the gear column 1507 rotates, it drives the transmission shaft block 1511 to reciprocate along the elliptical wall groove 1512, thereby driving the sliding disk 1508 to reciprocate.

[0031] Reference Figures 1-11 In the embodiment of the present invention, a method for concentrating a collagen solution for articular cartilage regeneration includes the following steps: S1: Start the controller 5 to heat the heating coil 6, and keep the temperature at 35 - 40 degrees Celsius. At the same time, introduce inert gas along the intake pipe 901, and start the filtering mechanism 15 to filter the passing inert gas. When the inert gas starts to enter the concentration tank 1, rotate the ball valve block 1102 to close the connecting wall pipe 10, and stop introducing the inert gas; S2: Start the vacuum pump 3 to pump out the air in the concentration tank 1, so that the air pressure in the concentration tank 1 is lower than -0.094 Mpa; S3: Start the electromagnetic stirrer 4 and continuously introduce inert gas again. The electromagnetic stirrer 4 drives the rotor 8 to rotate. The rotation of the rotor 8 drives the ball valve block 1102 to rotate. The rotation of the rotor 8 drives the stirring blade 1302 and the matching floating plate 1305 to rotate. Start the ultrafiltration membrane separator 2 to absorb small molecules such as evaporated water, and the collagen flows back into the concentration tank 1. It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0032] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for concentrating a collagen solution for regenerating articular cartilage, comprising a concentrating tank (1), an ultrafiltration membrane separator (2), a vacuum pump (3), and an electromagnetic stirrer (4) being installed on the top side of the concentrating tank (1), a heating coil (6) being installed on the inner and outer tank interlayers of the concentrating tank (1), an electric heating wire (7) being arranged inside the heating coil (6), a rotor (8) being arranged on the inner wall of the top side of the concentrating tank (1), and the rotor (8) being coupled to the electromagnetic stirrer (4), characterized in that: The heating coil (6) is provided with an aeration mechanism (9) for heating the inert gas and disturbing the water flow in the heating coil (6); a connecting wall tube (10) is provided on the top side of the concentration tank (1); the connecting wall tube (10) is connected to the aeration mechanism (9); a rotating mechanism (11) is provided on the rotor (8) for intermittently introducing the inert gas; the rotating mechanism (11) is located on one side of the connecting wall tube (10); a stirring hollow tube (12) is provided on the rotor (8); the stirring hollow tube (12) is connected to the rotating mechanism (11); an aeration mechanism (14) is provided on the stirring hollow tube (12) for uniformly introducing the inert gas; a stirring mechanism (13) is provided on the stirring hollow tube (12) for stirring the collagen solution; and a filtering mechanism (15) is provided on the aeration mechanism (9).

2. The device for concentrating collagen solution for articular cartilage regeneration according to claim 1, characterized in that: The ventilation mechanism (9) comprises an air inlet pipe (901), the air inlet pipe (901) being mounted on the heating coil (6), an air coil (902) being mounted on the air inlet pipe (901), the air coil (902) being located between the heating coil (6) and the electric heating wire (7), the air coil (902) being located on a side of the electric heating wire (7) close to the concentration tank (1), a mounting block (903) being mounted on the air coil (902), the mounting block (903) being mounted on the inner wall of the heating coil (6), an air drum membrane (904) being mounted on a side of the air coil (902) close to the concentration tank (1), an air outlet pipe (905) being mounted on the air coil (902), and the air outlet pipe (905) being mounted on the connecting wall pipe (10).

3. The device for concentrating collagen solution for articular cartilage regeneration according to claim 1, characterized in that: The rotating mechanism (11) comprises a through pipe (1101), the through pipe (1101) being mounted on the rotor (8), the through pipe (1101) being in communication with the stirring hollow pipe (12), a ball valve block (1102) being mounted on the through pipe (1101), an adapting ball groove (1104) being provided on the concentration tank (1), the ball valve block (1102) being rotatably mounted in the adapting ball groove (1104), an L-shaped curved hole (1103) being provided on the ball valve block (1102), and the L-shaped curved hole (1103) being in communication with the connecting wall pipe (10).

4. The device for concentrating collagen solution for articular cartilage regeneration according to claim 1, characterized in that: The aeration mechanism (14) comprises an aeration chamber (1401), the aeration chamber (1401) being mounted on the stirring hollow tube (12), the aeration chamber (1401) being provided with an air hole (1402), a sliding shaft block (1403) being mounted on the aeration chamber (1401), a piston disc (1404) being slidably mounted on the sliding shaft block (1403), the lower part of the piston disc (1404) being in contact with the inner wall of the aeration chamber (1401), the upper part of the piston disc (1404) being in contact with the inner wall of the stirring hollow tube (12), a spring (1405) being movably sleeved on the sliding shaft block (1403), and the spring (1405) being mounted on the piston disc (1404).

5. The device for concentrating collagen solution for articular cartilage regeneration according to claim 1, characterized in that: The stirring mechanism (13) comprises a cross bar (1301), the cross bar (1301) being mounted on the stirring hollow tube (12), stirring blades (1302) being mounted on the cross bar (1301), the stirring blades (1302) being symmetrically arranged and not in contact with each other, a top plate (1303) being mounted on the stirring hollow tube (12), a limit slide bar (1304) being slidably mounted on the top plate (1303), an adapting floating plate (1305) being mounted on the limit slide bar (1304), an arc-shaped wall (1306) being mounted on the adapting floating plate (1305), and the arc-shaped wall (1306) being in contact with the concentration tank (1).

6. The device for concentrating collagen solution for articular cartilage regeneration according to claim 2, characterized in that: The filtering mechanism (15) comprises a filter chamber (1501), the filter chamber (1501) being mounted on an air inlet pipe (901), a main roller (1502) being rotatably mounted on the filter chamber (1501), a filter membrane belt (1503) being movably sleeved on the main roller (1502), a driven roller (1504) being movably sleeved on the filter membrane belt (1503), the driven roller (1504) being rotatably mounted on the filter chamber (1501), and the air inlet pipe (901) being in contact with the filter membrane belt (1503).

7. The device for concentrating collagen solution for articular cartilage regeneration according to claim 6, characterized in that: A motor (1505) is installed on the filter chamber (1501), and a main shaft of the motor (1505) is installed on the main rotating roller (1502).

8. The device for concentrating collagen solution for articular cartilage regeneration according to claim 6, characterized in that: The main rotating roller (1502) is provided with a gear (1506), the filter chamber (1501) is rotatably provided with a gear column (1507), the gear column (1507) is meshed with the gear (1506), a sliding plate (1508) is slidably provided on the gear column (1507), a connecting shaft (1509) is provided on the sliding plate (1508), a membrane brush (1510) is provided on the connecting shaft (1509), and the membrane brush (1510) is in contact with the filter membrane belt (1503).

9. The device for concentrating collagen solution for articular cartilage regeneration according to claim 8, characterized in that: An elliptical wall groove (1512) is formed on the gear column (1507), a transmission shaft block (1511) is mounted on the sliding plate (1508), and the transmission shaft block (1511) is slidably mounted in the elliptical wall groove (1512).

10. A method for concentrating a collagen solution for regenerating articular cartilage, which is based on the device for concentrating a collagen solution for regenerating articular cartilage according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: starting the controller (5) to heat the heating coil (6) and maintaining the temperature at 35-40 degrees Celsius, while introducing inert gas along the air inlet pipe (901), starting the filtering mechanism (15) to filter the inert gas passing through, and when the inert gas starts to enter the concentration tank (1), the connecting wall pipe (10) is closed by rotating the ball valve block (1102), and the introduction of the inert gas is stopped; S2: starting the vacuum pump (3) to extract the air in the concentration tank (1) so that the air pressure in the concentration tank (1) is lower than -0.094 MPa; S3: The electromagnetic stirrer (4) is started and the inert gas is continuously introduced again. The electromagnetic stirrer (4) drives the rotor (8) to rotate. The rotation of the rotor (8) drives the ball valve block (1102) to rotate. The rotation of the rotor (8) drives the stirring blade (1302) and the adapter floating plate (1305) to rotate. The ultrafiltration membrane separator (2) is started to absorb the evaporated water and other small molecules, and the collagen flows back into the concentration tank (1).

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