Dendrobium officinale cold-resistant agent and application thereof

Abstract

The invention discloses a dendrobium officinale cold-resistant agent and application thereof. The active ingredient of the cold-resistant agent is folic acid and / or fulvic acid. Folic acid and fulvic acid are applied to cold-resistant treatment of dendrobium officinale for the first time, the innovation breaks through the limitation that a traditional cold-resistant agent is complex in component and possibly contains hormone or pesticide components, and a new, safer and more effective choice is provided for cold-resistant treatment of dendrobium officinale. The cold-resistant agent disclosed by the invention not only improves the survival rate of the dendrobium officinale in a low-temperature environment, but also remarkably reduces the damage degree of the appearance of the dendrobium officinale compared with a control group without using the cold-resistant agent, which shows that the cold-resistant agent disclosed by the invention has a remarkable effect on protecting the dendrobium officinale from low-temperature damage. The cold-resistant agent disclosed by the invention is simple in preparation process, easily available in raw materials and relatively low in cost, so that the cold-resistant agent has a wide application prospect in the fields of large-scale planting and deep processing of dendrobium officinale.

Description

Technical Field

[0001] The invention belongs to the technical field of plant protection, and particularly relates to a cold-resistant agent for Dendrobium officinale and application thereof. Background Art

[0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium in the orchid family. It is mainly distributed in southwestern Anhui, eastern Zhejiang, western Fujian, northwestern Guangxi, Sichuan and southeastern Yunnan in China. It prefers to grow on semi-humid rocks at an altitude of about 1,600 meters. This unique growth environment has created its extraordinary medicinal value. Dendrobium officinale contains rich bioactive ingredients such as polysaccharides, alkaloids, flavonoids and phenolic compounds, which make it have the effects of strengthening the stomach, replenishing yin, moistening the lungs, clearing eyes, clearing away heat and removing fire, and enhancing immunity.

[0003] However, in recent years, the germplasm resources of wild Dendrobium officinale are on the verge of extinction due to overharvesting and ecological damage. In order to meet the market demand for hundreds of millions of Dendrobium officinale plants each year, artificial breeding and planting are currently the main means of production. my country's Dendrobium officinale production bases are mainly concentrated in Zhejiang, Anhui and Yunnan. However, the lowest winter temperature in Zhejiang and Anhui can reach below 5°C, and the winter temperature in mountainous areas can even drop below 0°C. Studies have shown that low temperatures below 5°C will rapidly reduce the activity of Dendrobium officinale leaves. When the temperature drops below 0°C, the cells of Dendrobium officinale will be severely damaged, which will affect its normal growth and development.

[0004] In addition, the Dendrobium officinale industry still has the problem of blind breeding, especially in the Jiangsu, Zhejiang and Anhui regions. Due to the difficulty in adapting to the ecological and planting conditions of the introduction area, a large number of Dendrobium officinale are easily affected by low temperatures in winter and cannot survive the winter normally. This not only limits the growth and development of Dendrobium officinale, but even causes its death, which seriously restricts the reproduction and production of Dendrobium officinale. Therefore, how to improve the low temperature resistance of Dendrobium officinale has become an important issue that needs to be solved urgently. The cultivation of Dendrobium officinale urgently needs to find effective methods to deal with the threat of low temperatures, which is of great practical value for its expansion of cultivation in the north. Summary of the invention

[0005] The purpose of the present invention is to provide a cold-resistant agent for Dendrobium officinale and application thereof.

[0006] A cold-resistant agent for Dendrobium officinale, wherein the active ingredients of the cold-resistant agent are folic acid and / or fulvic acid.

[0007] Preferably, the active ingredient is folic acid or fulvic acid, and the dosage is 3-15 mg / L.

[0008] Preferably, the active ingredients are folic acid and fulvic acid, with the dosage of folic acid being 2 - 3 mg / L and the dosage of fulvic acid being 4 - 6 mg / L.

[0009] The dosage form of the cold tolerance agent is a spray, and 0.01 - 0.05% of surfactant is added during use.

[0010] The surfactant is Tween 20, Tween 80, Span 60 or NX - 7507.

[0011] Application of the cold tolerance agent in improving the survival rate of Dendrobium officinale under low temperature.

[0012] The low temperature is - 2°C - 8°C.

[0013] Advantages of the present invention: The present invention first applies folic acid and fulvic acid to the cold tolerance treatment of Dendrobium officinale. This innovation breaks the limitation of traditional cold resistance agents with complex components and possible hormone or pesticide components, providing a new, safer and more effective option for the cold tolerance treatment of Dendrobium officinale. The cold tolerance agent of the present invention not only improves the survival rate of Dendrobium officinale in low temperature environments, but also significantly reduces the degree of appearance damage compared to the control group without using the cold tolerance agent, indicating that the cold tolerance agent of the present invention is effective in protecting Dendrobium officinale from low temperature damage. The preparation process of the cold tolerance agent of the present invention is simple, the raw materials are easily available, and the cost is relatively low, which makes it have broad application prospects in the large - scale cultivation and deep processing fields of Dendrobium officinale. Description of the Drawings

[0014] Figure 1 Survival rate of Dendrobium officinale after treatment with different folic acid concentrations.

[0015] Figure 2 Survival rate of Dendrobium officinale after treatment with different fulvic acid concentrations.

[0016] Figure 3 Phenotypes of Dendrobium officinale before and after low - temperature treatment in the folic acid + fulvic acid group. Detailed Embodiments

[0017] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0018] Example 1

[0019] Preparation of folic acid solution: Dissolve 0.05 g of folic acid powder in 10 ml of purified water to prepare a mother liquor of folic acid. Take 0 mL, 0.5 mL, 1 ml, 2 mL, and 5 mL of the folic acid mother liquor respectively according to the required ratio, dilute with water, add 0.2 ml of Tween 20, stir well, and finally make up the volume to 1 L with water to prepare solutions with folic acid concentrations of 0.0 mg / L, 2.5 mg / L, 5.0 mg / L, 10 mg / L, and 25 mg / L. After preparation, transfer to a storage bottle and store in the dark.

[0020] Select two-year-old Dendrobium officinale with consistent growth for subsequent treatment. Spray the folic acid solution on the leaves until the liquid drips from both the front and back sides of the leaves. Spray once every seven days. After spraying 4 times, place it in a low-temperature light incubator for low-temperature treatment at -1°C. After 4 weeks of treatment, place it in a greenhouse (25°C) to resume growth for two weeks and then observe the phenotype. Count the survival rate of Dendrobium officinale. Use 30 Dendrobium officinale plants for each treatment, and repeat each treatment more than 3 times. The experimental results were statistically analyzed using SPSS 24.0 software. The measurement data results were expressed as x± (mean ± standard deviation). The Kolmogorov-Smirnov test method was used for data normality test. For data that conform to normal distribution, the t-test was used to compare the mean differences between two groups. A P<0.05 was considered statistically significant. The results are shown in Figure 1 .

[0021] It can be seen from Figure 1 that before low-temperature treatment: the survival rate of Dendrobium officinale under different folic acid concentrations (0.0 mg / L, 2.5 mg / L, 5.0 mg / L, 10 mg / L, 25 mg / L) was 100%, and there was no difference among the concentrations. After low-temperature treatment: the survival rate of Dendrobium officinale without folic acid treatment (0.0 mg / L) decreased significantly, much lower than that of other folic acid treatment groups. As the folic acid concentration increased, the survival rate showed a trend of first increasing and then decreasing. Among them, when the folic acid concentration was 5 mg / L, the survival rate was relatively high; the survival rates of the 25 mg / L and 10 mg / L folic acid treatment groups were slightly lower than that of the 5 mg / L group, but higher than those of other lower folic acid concentration groups. Generally speaking, treatment with an appropriate concentration of folic acid can improve the survival rate of Dendrobium officinale after low-temperature treatment.

[0022] Example 2

[0023] Preparation of fulvic acid solution: Dissolve 0.05 g of fulvic acid in 10 ml of purified water to prepare a fulvic acid stock solution. Take 0 mL, 0.5 mL, 1 ml, 2 mL, and 5 mL of the fulvic acid stock solution respectively according to the required ratio, dilute with water, add 0.2 ml of Tween 20, stir well, and finally make up the volume to 1 L with water to prepare solutions with fulvic acid concentration contents of 0.0 mg / L, 2.5 mg / L, 5.0 mg / L, 10 mg / L, and 25 mg / L respectively. After preparation, transfer to a storage bottle and store in the dark.

[0024] Select two-year-old Dendrobium officinale with consistent growth for subsequent treatment. Spray the fulvic acid solution on the leaves until the liquid drips from both the front and back sides of the leaves. Spray once every seven days. After spraying 4 times, place it in a low-temperature light incubator for low-temperature treatment at -1°C. After 4 weeks of treatment, place it in a greenhouse (25°C) to recover growth for two weeks and then observe the phenotype. Count the survival rate of Dendrobium officinale. Use 30 plants of Dendrobium officinale for each treatment, and repeat each treatment more than 3 times. The experimental results were statistically analyzed using SPSS 24.0 software. The results of measurement data were expressed as x± (mean ± standard deviation). The Kolmogorov-Smirnov test method was used for data normality test. For data that conform to normal distribution, the t-test was used to compare the mean differences between two groups. A P<0.05 was considered statistically significant. The results are shown in Figure 2 。

[0025] It can be seen from Figure 2 that before low-temperature treatment: the survival rate of Dendrobium officinale under different fulvic acid concentrations (0.0 mg / L, 2.5 mg / L, 5.0 mg / L, 10 mg / L, 25 mg / L) was 100%, and there was no difference among the concentrations. After low-temperature treatment: the survival rate of Dendrobium officinale without fulvic acid treatment (0.0 mg / L) decreased significantly, far lower than that of other fulvic acid treatment groups. With the increase of fulvic acid concentration, the survival rate showed a trend of first increasing and then decreasing. Among them, when the fulvic acid concentration was 10 mg / L, the survival rate was relatively high; the survival rate of the 25 mg / L fulvic acid treatment group was slightly lower than that of the 10 mg / L group, but higher than that of other lower fulvic acid concentration groups. Overall, treatment with an appropriate concentration of fulvic acid can improve the survival rate of Dendrobium officinale after low-temperature treatment.

[0026] Example 3

[0027] Solution preparation: Take 0.5 mL of the folic acid stock solution prepared in Example 1, 1 mL of the fulvic acid solution prepared in Example 2, dilute with water, add 0.2 mL of Tween 20, stir well and mix evenly, and finally make up the volume to 1 L with water to prepare a solution with a folic acid concentration of 2.5 mg / L and a fulvic acid concentration of 5.0 mg / L; Take 1.5 mL of the folic acid stock solution prepared in Example 1, dilute with water, add 0.2 mL of Tween 20, stir well and mix evenly, and finally make up the volume to 1 L with water to prepare a solution with a folic acid concentration of 7.5 mg / L; Take 1.5 mL of the fulvic acid stock solution prepared in Example 2, dilute with water, add 0.2 mL of Tween 20, stir well and mix evenly, and finally make up the volume to 1 L with water to prepare a solution with a fulvic acid concentration of 7.5 mg / L; After preparation, transfer to a storage bottle and store in the dark.

[0028] Select two-year-old Dendrobium officinale with consistent growth for subsequent treatment, and spray folic acid, fulvic acid, and folic acid + fulvic acid solutions on the leaves respectively until the liquid drips from both the front and back sides of the leaves. Once every seven days, after spraying 4 times, place in a low-temperature light incubator for low-temperature treatment at -1°C. After 4 weeks of treatment, place in a greenhouse (25°C) to resume growth for two weeks and then observe the phenotype ( Figure 3 ), count the survival rate of Dendrobium officinale. For each treatment, 30 plants of Dendrobium officinale are used, and each treatment is repeated more than 3 times. The experimental results are statistically analyzed using SPSS 24.0 software. The results of measurement data are expressed as x± (mean ± standard deviation). The Kolmogorov-Smirnov test method is used for data normality test. For data that conform to normal distribution, the t-test is used to compare the mean differences between two groups. A P < 0.05 is considered statistically significant. The results are shown in Figure 3 Table 1.

[0029] Table 1

[0030]

[0031] Note: * represents P < 0.05 compared with the folic acid + fulvic acid group.

[0032] It can be seen from Figure 3 that there are obvious differences in the phenotypes of Dendrobium officinale before and after low-temperature treatment in the folic acid + fulvic acid group. It can be seen from Table 1 that after low-temperature treatment, the survival rate of the folic acid + fulvic acid group is significantly higher than that of the folic acid group and the fulvic acid group, proving that the combined use of folic acid and fulvic acid has a synergistic effect.

[0033] The above-described embodiments merely represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. A cold-resistant agent for Dendrobium officinale, characterized in that: The active ingredients of the cold-resistant agent are folic acid and / or fulvic acid.

2. The cold-resistant Dendrobium officinale agent according to claim 1, characterized in that: The active ingredient is folic acid or fulvic acid, and the dosage is 3-15 mg / L.

3. The cold-resistant Dendrobium officinale agent according to claim 1, characterized in that: The active ingredients are folic acid and fulvic acid, the folic acid dosage is 2-3 mg / L, and the fulvic acid dosage is 4-6 mg / L.

4. The cold-resistant Dendrobium officinale agent according to claim 1, characterized in that: The cold-resistant agent is in the form of a spray, and 0.01-0.05% of a surfactant is added when used.

5. The cold-resistant agent of Dendrobium officinale according to claim 1, characterized in that: The surfactant is Tween 20, Tween 80, Span 60 or NX-7507.

6. Use of the cold-resistant agent according to claim 1 in improving the survival rate of Dendrobium officinale at low temperatures.

7. The use of the cold-resistant agent according to claim 1 in improving the survival rate of Dendrobium officinale at low temperatures, characterized in that: The low temperature is -2°C-8°C.

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