A cleansing, antibacterial, and anti-inflammatory composition and its preparation method

Abstract

This invention discloses a cleaning, antibacterial, and anti-inflammatory composition and its preparation method, specifically belonging to the field of daily cleaning and care. The composition comprises the following components in parts by weight: 10-18 parts surfactant, 3-5 parts plant extract, and the remainder being excipients and solvent. The surfactant is an amino acid-type surfactant, cocamidopropyl betaine, and cocamidomethyl MEA in a mass ratio of 10:(0.5-2):(2-3). The plant extract is Rubia cordifolia extract, St. John's wort extract, and Cyperus rotundus extract in a mass ratio of 1:(4-5):(1-3). The plant extract provided by this invention exhibits a significant synergistic effect in antibacterial and anti-inflammatory properties, effectively improving the overall antibacterial, antimicrobial, and anti-inflammatory effects of the formula. This invention also found that when potassium cocoyl glycinate, cocamidopropyl betaine, and cocamidomethyl MEA are used in a specific ratio, they can effectively enhance the detergency of the formula.

Description

Technical Field

[0001] This invention belongs to the field of daily cleaning and care products, specifically relating to a cleaning, antibacterial, and anti-inflammatory composition and its preparation method. Background Technology

[0002] In daily life, the demand for personal care products, cleaning supplies, and cosmetics is increasing daily, and consumers are also demanding higher standards from these products. They not only pursue effective cleaning but also pay more attention to the gentleness, safety, and environmental friendliness of the products. Therefore, developing high-performance, skin-friendly compositions has become a research hotspot in the industry.

[0003] Surfactants, as key components in these products, play multiple roles, including cleaning, foaming, and emulsification. However, traditional surfactants, such as soap-based surfactants, while possessing strong cleaning power, often cause significant skin irritation, leading to dryness and tightness with prolonged use. To address this issue, the industry has begun seeking milder, less irritating surfactants and experimenting with compounding them with other beneficial ingredients to create compositions that offer both excellent cleaning performance and gentle, non-irritating properties.

[0004] Amino acid-based surfactants, with their excellent mildness, low irritation, and good biodegradability, are gradually becoming a preferred alternative to traditional soap-based surfactants. However, using amino acid-based surfactants alone may not meet the specific requirements of some products regarding cleaning power, foam stability, etc. Therefore, it is necessary to compound them with other surfactants to fully utilize their respective advantages and achieve better results.

[0005] Furthermore, with the increasing demand for natural and organic products, adding plant extracts to compositions has become a trend. Plant extracts not only add a natural and refreshing scent to products but may also possess various benefits such as antibacterial, anti-inflammatory, and moisturizing properties, thereby enhancing the overall quality of the product. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a cleansing, antibacterial, and anti-inflammatory composition and its preparation method. This composition is prepared by compounding amino acid-type surfactants with other surfactants in a specific ratio, and adding plant extracts with specific effects. The aim is to prepare a novel composition that has both good cleansing effect and is gentle and non-irritating, while also having highly effective antibacterial, anti-inflammatory, and moisturizing properties, in order to meet the market demand for high-quality personal care products, cleaning products, and cosmetics.

[0007] To achieve the above objectives, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a cleaning, antibacterial, and anti-inflammatory composition, comprising, by weight parts:

[0009] 10-18 parts of surfactant;

[0010] 3-5 parts of plant extract;

[0011] Add excipients and solvents to a total of 100 parts;

[0012] The surfactant is an amino acid-type surfactant, cocamidopropyl betaine, or cocamidomethyl MEA in a mass ratio of 10:(0.5-2):(2-3), wherein the amino acid-type surfactant is selected from at least one of potassium cocoyl glycinate, sodium cocoyl glycinate, or sodium cocoyl glutamate.

[0013] The plant extracts are Rubia cordifolia extract, St. John's wort extract and Cyperus rotundus extract in a mass ratio of 1:(4-5):(1-3).

[0014] Preferably, the Cyperus rotundus extract is prepared by the following method:

[0015] Step 1-1. Raw material pretreatment: The dried Cyperus rotundus medicinal material is pulverized by a high-speed pulverizer and then passed through a 60-mesh sieve to obtain coarse Cyperus rotundus powder;

[0016] Step 1-2. Solvent extraction: Mix the crude powder of Cyperus rotundus with a 75 v / v% ethanol aqueous solution at a material-to-liquid ratio of 1:20 g / mL, and reflux extract at 80℃ for 3 times, 1 hour each time. After extraction, filter with a 120-mesh filter cloth and combine the filtrates.

[0017] Steps 1-3. Concentration: The combined filtrate is concentrated under reduced pressure until alcohol-free. The concentration conditions are 60℃ and 0.06MPa. The concentrated filtrate is then evaporated in an 80℃ water bath until it becomes a paste.

[0018] Steps 1-4. Drying: Place the extract in a vacuum drying oven and dry it at a temperature of 70℃ and a pressure of 0.08MPa until the moisture content is ≤10%. Grind it into powder and pass it through a 60-mesh sieve to obtain Cyperus rotundus extract.

[0019] The solvent is at least one of 1,3-butanediol, 1,3-propanediol, 1,2-propanediol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol, dipropylene glycol, glycerol, and deionized water.

[0020] Preferably, the excipients are selected from at least one of antioxidants, thickeners, pH adjusters, preservatives, and flavorings.

[0021] More preferably, the antioxidant is selected from at least one of L-ascorbic acid and vitamin E.

[0022] More preferably, the thickener is selected from at least one of xanthan gum, sodium chloride, and carbomer.

[0023] More preferably, the pH adjuster is selected from salicylic acid, lactic acid, citric acid, and arginine.

[0024] More preferably, the preservative is at least one of p-hydroxyacetophenone and phenoxyethanol.

[0025] In a second aspect, the present invention provides the use of the composition described in the first aspect in the preparation of facial cleansing products having antibacterial, anti-inflammatory, cleansing and / or moisturizing effects.

[0026] Thirdly, the present invention provides a method for preparing the composition described in the first aspect, comprising the following steps:

[0027] Step 2-1. Mix the surfactant, thickener, and solvent at 80-85℃ until homogeneous to obtain the first mixture;

[0028] Step 2-2. Cool the first mixture to 45-50℃, add the preservative and mix until homogeneous to obtain the second mixture;

[0029] Steps 2-3: Cool the second mixture to 35-40°C, add plant extracts, antioxidants, pH adjusters, and fragrances, and mix until homogeneous to obtain the composition.

[0030] In this invention:

[0031] The main components of madder extract include anthraquinones, naphthoquinones, iridoids, terpenes, and polysaccharides. These components endow madder extract with the ability to inhibit various pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Salmonella. In addition, madder extract also has anti-inflammatory effects, helping to soothe skin problems, promote skin repair, and make the skin healthier.

[0032] St. John's wort extract is rich in benzodiathrone compounds, such as hypericin, pseudohypericin, and protohypericin. These components give St. John's wort extract significant antibacterial activity against a variety of bacteria, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Klebsiella pneumoniae. At the same time, St. John's wort extract also has anti-inflammatory effects, which can inhibit the production of inflammatory mediators, reduce skin inflammation, and provide comprehensive protection for the skin.

[0033] The main components of Cyperus rotundus extract include terpenes, flavonoids, alkaloids, sugars, and sterols. These components endow the extract with the ability to inhibit various bacteria, helping to prevent skin infections. Furthermore, Cyperus rotundus extract can inhibit the production of inflammatory mediators, exhibiting anti-inflammatory effects, reducing skin inflammation, alleviating skin problems, and making the skin healthier and more comfortable.

[0034] Amino acid surfactants possess excellent detergency, emulsification, dispersion, wetting, penetration, solubilization, foaming, and foam stabilization properties. Cocamidopropyl betaine, an amphoteric surfactant, exhibits low irritation, excellent detergency, rich foam, and good thickening properties. Cocamidomethyl MEA, a nonionic surfactant, possesses excellent thickening properties and demonstrates good detergency in various liquid detergents. When these three ingredients are used in combination, they synergistically enhance detergency. Amino acid surfactants provide gentle cleaning power, cocamidopropyl betaine increases foam richness and stability, while cocamidomethyl MEA further improves the product's detergency and thickening properties. This compound system not only effectively removes dirt and oil from the skin's surface but also retains skin moisture, reduces skin irritation, and leaves the skin feeling refreshed and comfortable after using the cleanser.

[0035] The beneficial effects of this invention are:

[0036] 1. The plant extracts provided by this invention, including Rubia cordifolia extract, St. John's wort extract, and Cyperus rotundus extract, exhibit significant synergistic effects in antibacterial and anti-inflammatory properties, effectively improving the overall antibacterial, antimicrobial, and anti-inflammatory effects of the formulation.

[0037] 2. When the amino acid-type surfactant, cocamidopropyl betaine, and cocamidomethyl MEA in the surfactant provided by this invention are used in a specific ratio, they can effectively improve the detergency of the formulation. Detailed Implementation

[0038] Those skilled in the art can refer to the content of this document to appropriately improve the process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included in this invention.

[0039] The products and methods of the present invention have been described through preferred embodiments. Those skilled in the art will be able to make modifications or appropriate alterations and combinations to the products and methods described herein without departing from the content, spirit and scope of the present invention, so as to realize and apply the technology of the present invention.

[0040] Experimental methods in the following examples, unless otherwise specified, are generally performed under standard conditions or as recommended by the manufacturer. Materials, reagents, etc., used are commercially available unless otherwise specified. Unless otherwise stated, substances described in this invention are calculated as percentages and parts by mass.

[0041] In this invention:

[0042] Rubia cordifolia extract: Rubia cordifolia extract, purchased from Xi'an Changyue Biotechnology Co., Ltd., product specification: 10:1;

[0043] Hypericum perforatum extract: Hypericum perforatum extract was purchased from Shaanxi Chenming Biotechnology Co., Ltd.

[0044] Cyperus rotundus: The dried medicinal material Cyperus rotundus was purchased from Bozhou Quanwei Pharmaceutical Co., Ltd.;

[0045] Other raw materials are all commercially available.

[0046] Preparation of Cyperus rotundus extract

[0047] Step 1-1. Raw material pretreatment: The dried Cyperus rotundus medicinal material is pulverized by a high-speed pulverizer and then passed through a 60-mesh sieve to obtain coarse Cyperus rotundus powder;

[0048] Step 1-2. Solvent extraction: Mix the crude powder of Cyperus rotundus with a 75 v / v% ethanol aqueous solution at a material-to-liquid ratio of 1:20 g / mL, and reflux extract at 80℃ for 3 times, 1 hour each time. After extraction, filter with a 120-mesh filter cloth and combine the filtrates.

[0049] Steps 1-3. Concentration: The combined filtrate is concentrated under reduced pressure until alcohol-free. The concentration conditions are 60℃ and 0.06MPa. The concentrated filtrate is then evaporated in an 80℃ water bath until it becomes a paste.

[0050] Steps 1-4. Drying: Place the extract in a vacuum drying oven and dry it at a temperature of 70℃ and a pressure of 0.08MPa until the moisture content is ≤10%. Grind it into powder and pass it through a 60-mesh sieve to obtain Cyperus rotundus extract.

[0051] Preparation of plant extracts

[0052] The raw materials were accurately weighed according to the mass ratio in Table 1, and then mixed evenly in a high-speed mixer to obtain the plant extract components.

[0053] Table 1. Mass ratio of plant extract components from raw materials

[0054]

[0055] Note: "-" in the table indicates no additions.

[0056] To verify the antibacterial and anti-inflammatory properties of the plant extracts, and to verify the key roles and interactions of each component in the plant extracts, adjustments were made to the raw materials and proportions based on plant extract 2, and the following antibacterial performance tests and inflammatory factor inhibition experiments were conducted.

[0057] Antibacterial performance test

[0058] Preparation of test sample solution: Take plant extract components 1-8 and mix them with deionized water and stir evenly to prepare a test sample solution with a concentration of 8wt%.

[0059] The bacterial strains used were Staphylococcus aureus GDMCC NO.: 1.1220 and Escherichia coli GDMCC NO.: 1.1917, which were purchased from the Guangdong Provincial Microbial Culture Collection Center.

[0060] The antibacterial test was conducted according to item 5.1.1 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antimicrobial Effects" for quantitative suspension antibacterial test. The test results are shown in Table 2.

[0061] Table 2. Results of antibacterial rate

[0062]

[0063] Inflammatory factor inhibition test

[0064] Sample solution to be tested: The plant extract was diluted to a concentration of 8 wt% using physiological saline.

[0065] Test method:

[0066] 1. Using RAW264.7 macrophages as the research subject, a cellular inflammation model was established by stimulating cells with lipopolysaccharide (LPS) (bacterial endotoxin).

[0067] 2. Macrophages were seeded into 12-well plates and incubated at 37°C and 5 v / v% CO2 for 24 h. The test sample solution was added, and LPS (1 μg / mL) was added 2 h later. This was recorded as T1. A group was also set up where only LPS was added, without the test sample solution, and this group was recorded as T1. 总 The group with only the sample solution added and no LPS added was designated as T0. After stimulation for 24 hours, the supernatant was collected, centrifuged, and detected.

[0068] 3. The release level of the pro-inflammatory cytokine TNF-α in RAW264.7 was analyzed using an ELISA kit. The TNF-α inhibition rate was calculated using the following formula:

[0069] TNF-α inhibition rate (%) = [(T 总-T1) / (T 总 -T0)]×100%.

[0070] A higher TNF-α inhibition rate indicates a better anti-inflammatory effect of the yeast fermentation product filtrate. The TNF-α inhibition test results are shown in Table 3.

[0071] Table 3. TNF-α Inhibition Rate

[0072]

[0073] Results analysis:

[0074] As shown in Tables 2 and 3, the extracts of Rubia cordifolia, St. John's wort, and Cyperus rotundus exhibit significant synergistic effects in antibacterial and anti-inflammatory properties. Experimental data indicate that when these three extracts coexist within a certain ratio, the antibacterial rate against Staphylococcus aureus and Escherichia coli exceeds 99%, and the TNF-α inhibition rate reaches over 30%. Conversely, the absence of any one component leads to a significant decrease in the antibacterial rate and a marked weakening of the anti-inflammatory effect. In conclusion, the three extracts of Rubia cordifolia, St. John's wort, and Cyperus rotundus must be used in a specific ratio to synergistically enhance the antibacterial and anti-inflammatory properties of the composition; all three are indispensable.

[0075] Preparation of antibacterial and anti-inflammatory facial cleanser

[0076] Weigh the raw materials precisely according to the mass fractions in Table 5;

[0077] Surfactant, xanthan gum, glycerin and deionized water were mixed at 85°C until homogeneous to obtain the first mixture; wherein the surfactant was compounded according to a specific mass ratio between amino acid type surfactant, cocamidopropyl betaine and cocamidomethyl MEA, as shown in Table 4.

[0078] Table 4 Mass ratio of surfactant components

[0079]

[0080] Note: "-" in the table indicates no additions.

[0081] The first mixture was cooled to 45°C, and p-hydroxyacetophenone was added and mixed until homogeneous to obtain the second mixture;

[0082] The second mixture was cooled to 40°C, and plant extract component 2, L-ascorbic acid, arginine, and fragrance were added and mixed until homogeneous to obtain the antibacterial and anti-inflammatory facial cleanser.

[0083] Table 5. Mass parts of raw materials for antibacterial and anti-inflammatory facial cleansers

[0084]

[0085]

[0086] Note: "-" in the table indicates no additions.

[0087] Stimulation test

[0088] The experimental method was in accordance with SN / T 2329-2009 "Eye Irritation / Corrosivity Test of Chicken Embryo Villi Allantoic Membrane in Cosmetics". Irritation tests were conducted on the antibacterial and anti-inflammatory facial cleansers of Examples 1-3 and Comparative Examples 1-3. The test results showed that the IS score of all tested samples was <1, and the tested samples were non-irritating.

[0089] Stain removal performance test

[0090] Test substances: Examples 1-3 and Comparative Examples 1-3 prepared above were used as test substances.

[0091] Grouping: Sixty healthy skin subjects were selected and randomly divided into 6 groups of 10 each. Each group was tested with the corresponding test substance.

[0092] Test procedure: Apply 3cm×1cm portions of oil-based lipstick and eyebrow pencil to the inner 4cm×5cm area of ​​the left and right arms respectively. Apply 0.5g of the test substance to the stains on both arms, gently rub for 1 minute, rinse off the foam with warm water, and dry with a paper towel.

[0093] Evaluation criteria: Before and after the test, photos were taken and compared. Grid count was used. Lipstick and eyebrow pencil stains were completely or nearly completely removed as excellent. Removal of more than 1 / 2 but still leaving a mark was good. Removal of 1 / 3-1 / 2 but still leaving a noticeable mark was poor. Removal of less than 1 / 3 was ineffective. This evaluation was conducted by a dedicated professional evaluator. The test results are shown in Table 6.

[0094] Table 6. Cleaning effect

[0095] Grouping Excellent / person good / person Poor / person Invalid / person Example 1 6 4 0 0 Example 2 8 2 0 0 Example 3 10 0 0 0 Comparative Example 1 0 5 5 0 Comparative Example 2 0 6 4 0 Comparative Example 3 0 9 1 0

[0096] Results analysis:

[0097] The experimental results show that the detergency of Examples 1, 2, and 3 gradually increases, proving that the higher the concentration of surfactant in the antibacterial and anti-inflammatory facial cleanser, the better the detergency, showing a positive correlation. The detergency of Comparative Examples 1-3 is not ideal, indicating that when amino acid surfactants, cocamidopropyl betaine, and cocamidomethyl MEA are used in a specific ratio, they can effectively improve the detergency of the formula. The absence of any one of the ingredients or the change in the ratio of the three will lead to poor detergency.

[0098] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. A cleansing, antibacterial, and anti-inflammatory composition, characterized in that, By mass, it includes the following components: 10-18 parts of surfactant; 3-5 parts of plant extract; Add excipients and solvents to a total of 100 parts; The surfactant is an amino acid-type surfactant, cocamidopropyl betaine, or cocamidomethyl MEA in a mass ratio of 10:(0.5-2):(2-3), wherein the amino acid-type surfactant is selected from at least one of potassium cocoyl glycinate, sodium cocoyl glycinate, or sodium cocoyl glutamate. The plant extracts are Rubia cordifolia extract, St. John's wort extract and Cyperus rotundus extract in a mass ratio of 1:(4-5):(1-3). The solvent is at least one of 1,3-butanediol, 1,3-propanediol, 1,2-propanediol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol, dipropylene glycol, glycerol, and deionized water. The Cyperus rotundus extract is prepared by the following method: Step 1-1. Raw material pretreatment: The dried Cyperus rotundus medicinal material is pulverized by a high-speed pulverizer and then passed through a 60-mesh sieve to obtain coarse Cyperus rotundus powder; Step 1-2. Solvent extraction: Mix the crude powder of Cyperus rotundus with a 75 v / v% ethanol aqueous solution at a material-to-liquid ratio of 1:20 g / mL, and reflux extract at 80℃ for 3 times, 1 hour each time. After extraction, filter with a 120-mesh filter cloth and combine the filtrates. Steps 1-3. Concentration: The combined filtrate is concentrated under reduced pressure until alcohol-free. The concentration conditions are 60℃ and 0.06MPa. The concentrated filtrate is then evaporated in an 80℃ water bath until it becomes a paste. Steps 1-4. Drying: Place the extract in a vacuum drying oven and dry it at a temperature of 70℃ and a pressure of 0.08MPa until the moisture content is ≤10%. Grind it into powder and pass it through a 60-mesh sieve to obtain Cyperus rotundus extract. The madder extract was obtained from Xi'an Changyue Biotechnology Co., Ltd.; The extract of St. John's wort was obtained from Shaanxi Chenming Biotechnology Co., Ltd.

2. The cleaning, antibacterial, and anti-inflammatory composition according to claim 1, characterized in that, The excipients are selected from at least one of antioxidants, thickeners, pH adjusters, preservatives, and flavorings.

3. The cleaning, antibacterial, and anti-inflammatory composition according to claim 2, characterized in that, The antioxidant is selected from at least one of L-ascorbic acid and vitamin E.

4. The cleaning, antibacterial, and anti-inflammatory composition according to claim 2, characterized in that, The thickener is selected from at least one of xanthan gum, sodium chloride, and carbomer.

5. The cleaning, antibacterial, and anti-inflammatory composition according to claim 2, characterized in that, The pH adjuster is selected from salicylic acid, lactic acid, citric acid, and arginine.

6. The cleaning, antibacterial, and anti-inflammatory composition according to claim 2, characterized in that, The preservative is at least one of p-hydroxyacetophenone and phenoxyethanol.

7. The use of the cleansing, antibacterial and anti-inflammatory composition of any one of claims 1-6 in the preparation of facial cleansing products having antibacterial, anti-inflammatory, cleansing and / or moisturizing effects.

8. A method for preparing the composition according to any one of claims 2-6, characterized in that, Includes the following steps: Step 2-1. Mix the surfactant, thickener, and solvent at 80-85℃ until homogeneous to obtain the first mixture; Step 2-2. Cool the first mixture to 45-50℃, add the preservative and mix until homogeneous to obtain the second mixture; Steps 2-3. Cool the second mixture to 35-40℃, add the plant extract, antioxidant, pH adjuster, and fragrance, and mix until homogeneous to obtain the composition.

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