Portable microbial gene rapid detection equipment

Abstract

The invention provides portable rapid microbial gene detection equipment. The portable rapid microbial gene detection equipment comprises an integrated device, an oscillation instrument, a vortex instrument, a DNA extraction part, a quality tester and a portable sequencer, the integrated device is internally provided with a plurality of mounting spaces, and the oscillator, the vortex instrument, the DNA extraction part, the quality tester and the portable sequencer are respectively arranged in the corresponding mounting spaces; the integrated device is provided with a power supply module, and the power supply module is electrically connected to the oscillator, the vortex instrument, the quality tester and the portable sequencer so as to supply power to instruments electrically connected with the power supply module. The portable microbial gene rapid detection equipment provided by the invention can meet the requirement of long-term field detection, can complete multiple steps in gene detection on site, does not need to return to a laboratory for analysis, avoids sample degradation or pollution caused by transportation and storage, ensures the accuracy of a detection result, and is convenient to use. Meanwhile, a large amount of time cost is also reduced, and the equipment has good rapid detection capability.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, and particularly to a portable rapid microbial gene detection device. Background Art

[0002] Gene detection plays a crucial role in fields such as life science research and environmental detection. In terms of environmental detection, gene detection can be used to study the microbial community structure and function in the environment, as well as to evaluate the health status of the ecosystem, etc. However, most existing gene detections rely on being carried out in the laboratory, lacking rapid detection devices that can be directly applied on-site.

[0003] The process of laboratory gene detection involves multiple steps such as sample collection, DNA extraction, library construction, laboratory high-throughput sequencing, and subsequent data analysis. It not only takes a long time, but also highly depends on laboratory conditions, and has strict requirements for equipment transportation and sample storage. Especially in special environments such as high altitudes, due to the influence of low temperature and low pressure climates, and the long time for sample transportation, the existing detection methods are prone to data distortion, ultimately affecting the accuracy of the detection results.

[0004] For example, existing desktop high-throughput sequencing devices, such as the Illumina MiSeq or HiSeq series, although they have high sensitivity and high-throughput detection capabilities, can only operate in a laboratory environment and cannot achieve on-site detection. And some detection devices need to rely on a variety of laboratory auxiliary devices, and the operation is complex, making it difficult to be applicable to independent field environments. These problems severely limit the application of gene detection in outdoor on-site rapid detection.

[0005] Based on this, this application hopes to propose a portable rapid microbial gene detection device to solve at least one of the above problems existing in the prior art. Summary of the Invention

[0006] In order to overcome the above defects of the prior art, the present invention provides a portable rapid microbial gene detection device.

[0007] The specific technical solution is as follows:

[0008] A portable rapid microbial gene detection device includes: an integrated device, a shaker, a vortex mixer, a DNA extractor, a quality detector, and a portable sequencer;

[0009] The interior of the integrated device has multiple installation spaces, and the shaker, the vortex mixer, the DNA extractor, the quality detector, and the portable sequencer are respectively arranged in their corresponding installation spaces;

[0010] The integrated device has a power supply module, which is electrically connected to the oscillator, the mediator, the mass spectrometer, and the portable sequencer to supply power to the instruments electrically connected thereto.

[0011] In a specific embodiment, a solar panel is disposed outside the integrated device, and the solar panel is electrically connected to the power supply module.

[0012] In a specific embodiment, the integrated device includes an instrument box with an openable cover, and the power supply module is disposed in the instrument box;

[0013] A flexible member is disposed in the instrument box, and a plurality of grooves are formed in the flexible member. The oscillator, the mediator, the DNA extractor, the mass spectrometer, and the portable sequencer are respectively detachably disposed in their corresponding grooves.

[0014] In a specific embodiment, a temperature sensor is disposed in the instrument box, and an electric heater is disposed in the wall of at least one of the grooves. The temperature sensor and the electric heater are both electrically connected to the power supply module, and the electric heater is communicatively connected to the temperature sensor for turning on heating when the internal temperature of the instrument box is lower than a preset temperature.

[0015] In a specific embodiment, a pressure relief valve for adjusting the internal pressure of the instrument box is disposed on the instrument box.

[0016] In a specific embodiment, the flexible member is detachably disposed in the instrument box;

[0017] And / or, at least one of the oscillator, the mediator, the DNA extractor, the mass spectrometer, and the portable sequencer is detachably disposed in the groove in a manner of interference fit with the flexible member.

[0018] In a specific embodiment, the length of the instrument box ranges from 45 cm to 55 cm;

[0019] And / or, the width of the instrument box ranges from 25 cm to 35 cm;

[0020] And / or, the height of the instrument box ranges from 15 cm to 25 cm.

[0021] In a specific embodiment, at least one of the cover and the instrument box is provided with a sealing member for achieving a sealed connection between the cover and the instrument box when the cover is covered on the instrument box;

[0022] And / or, a heat-insulating buffer member is disposed on the side of the cover close to the instrument box.

[0023] In a specific embodiment, the instrument case is made of any one or a combination of carbon fiber composite material, aluminum alloy material, and titanium alloy material.

[0024] In a specific embodiment, a grip for human hand to hold is provided on the integrated device.

[0025] The present invention has at least the following beneficial effects:

[0026] In summary, a portable rapid microbial gene detection device provided by the present invention integrates a shaker, a vortex mixer, a DNA extraction component, a quality detector, and a portable sequencer on an integrated device, making the device highly integrated and convenient for carrying outdoors. Moreover, the integrated device can supply power to each instrument through a power supply module, meeting the needs of long-term field detection. And, based on the integration of various components on the integrated device, multiple steps in genomic and functional gene detection can be completed on-site without returning to the laboratory for analysis, avoiding sample degradation or contamination caused by transportation and storage, ensuring the authenticity of data and the accuracy of detection results. At the same time, a large amount of time cost is reduced. The device has good rapid detection ability, and compared with traditional detection technologies, the detection efficiency of this device is significantly improved.

[0027] Furthermore, the shaker, the vortex mixer, the DNA extraction component, the quality detector, and the portable sequencer are all arranged in a detachable form, which is convenient to select and replace components according to needs, and has good flexibility. The flexible component can also be arranged in a detachable form, and the flexible component with different grooves can be flexibly replaced to adapt to various components of different models by changing the shape of the grooves.

[0028] Furthermore, in view of the inconvenient charging in the wild, this device can convert light energy into electrical energy through a solar panel and collect it through a battery. Thus, in addition to being powered by the built-in battery, this device also supports external solar panel power supply, so as to be able to adapt to long-term field applications.

[0029] Furthermore, in view of the application in a low-temperature environment, this device can judge the temperature through a temperature sensor and heat through an electric heater to keep the temperature in the instrument case within a certain range, which is beneficial to ensuring the normal operation of the quality detector and the portable sequencer, and further improving the accuracy of detection results. And, the instrument is not easily affected by the external low temperature and can perform gene detection work at any time, which also greatly improves the convenience of using the device and further reduces the time cost of detection.

[0030] Furthermore, for the case of being applied to a low-pressure environment, through the setting of a pressure relief valve, this device can balance the air pressure inside and outside the instrument box, thus solving the problem of the instrument box expanding in a low-pressure environment.

[0031] Furthermore, the length range of the instrument box can be between 45 cm and 55 cm, the width range can be between 25 cm and 35 cm, the height range can be between 15 cm and 25 cm, and the weight of the instrument box is within 10 kg, being relatively light and providing a good user experience. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0033] Figure 1 It is a schematic diagram of the overall structure of the portable microbial gene rapid detection device of the present invention;

[0034] Figure 2 It is a schematic diagram of the overall structure of the integrated device of the present invention;

[0035] Figure 3 It is a schematic diagram of the side perspective view of the integrated device of the present invention;

[0036] Figure 4 It is a schematic diagram of the shape of the flexible part groove of the present invention;

[0037] Figure 5 It is a schematic diagram of sampling points and metagenomic annotation.

[0038] REFERENCE NUMERALS:

[0039] 1 - Integrated device; 11 - Instrument box; 12 - Cover; 2 - Shaker; 3 - Vortexer; 4 - DNA extraction part; 5 - Mass spectrometer; 6 - Portable sequencer; 7 - Power supply module; 71 - Battery; 8 - Flexible part; 81 - Groove; 9 - Solar panel; 10 - Temperature sensor; 13 - Electric heater; 14 - Pressure relief valve; 15 - Sealing ring; 16 - Thermal insulation and buffer part; 17 - Grip; 18 - Buckle. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0040] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0041] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "vertical", "upper", "lower", "horizontal", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0042] In the description of the present invention, it should also be noted that unless otherwise clearly defined and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0043] At present, the technologies for detecting microbial genomes or functional genes in environmental samples mostly focus on laboratory analysis, lacking equipment that can be directly applied to on-site detection. Laboratory analysis usually requires complex experimental steps, including sample collection, DNA extraction, library construction, laboratory high-throughput sequencing, and subsequent data analysis, etc. These steps are time-consuming, rely on laboratory conditions, and cannot achieve on-site rapid detection. It is necessary to transport the samples back to the laboratory for analysis after collection, which has high requirements for equipment transportation and sample storage. Deviations may be introduced during the storage and transportation of the samples, especially in special environments with high altitude and long transportation time, which is likely to cause data distortion, thus affecting the accuracy of the detection results. Moreover, the existing detection equipment is large in volume and heavy in weight, and it is also difficult to adapt to the low temperature and low pressure environments in high altitude areas, making it inconvenient to use. Based on this, the present application provides a portable rapid microbial gene detection device, aiming to solve the problems existing in the above-mentioned prior art, and will be described in detail through specific embodiments below.

[0044] Please refer to Figures 1 to 4 , the present invention provides a portable rapid microbial gene detection device, including: an integrated device 1, a shaker 2, a vortex mixer 3, a DNA extraction component 4, a quality detector 5, and a portable sequencer 6.

[0045] Specifically, the interior of the integrated device 1 has multiple installation spaces, and the shaker 2, vortex mixer 3, DNA extraction component 4, mass spectrometer 5, and portable sequencer 6 are respectively arranged in their corresponding installation spaces. The integrated device 1 has a power supply module 7, and the power supply module 7 is electrically connected to the shaker 2, vortex mixer 3, mass spectrometer 5, and portable sequencer 6 to supply power to the instruments electrically connected thereto.

[0046] The functions of the above-mentioned multiple components will be introduced separately below.

[0047] The shaker 2 is used to mix and preliminarily lyse the collected samples.

[0048] The vortex mixer 3 is used to generate a vortex motion by high-speed rotation to promote cell lysis and DNA release in the samples.

[0049] The DNA extraction component 4 is used for nucleic acid extraction. Exemplarily, the DNA extraction component 4 can be a DNA extraction kit, and the DNA extraction kit can be adaptively adjusted according to the sample type, and the sample type includes but is not limited to water samples, soil samples, etc.

[0050] The mass spectrometer 5 is used to quickly quantify the concentration of the extracted nucleic acid. Exemplarily, the mass spectrometer 5 can be a Qubit-DNA mass spectrometer.

[0051] The portable sequencer 6 is used to realize real-time sequencing analysis of in-situ genomes or functional genes. Exemplarily, the portable sequencer 6 is a portable MinION third-generation sequencer or a Flongle portable sequencer.

[0052] The usage method of the portable microbial gene rapid detection device of the present invention will be specifically introduced below. During actual use, the tester first collects on-site samples. After the sample sampling is completed, the samples are lysed by the shaker 2 and vortex mixer 3 built in the integrated device 1 to promote cell lysis and DNA release in the samples. After the lysis step is completed, the DNA extraction component 4 is used to extract nucleic acids from the samples, and the mass spectrometer 5 is used to detect the concentration of the extracted nucleic acids. Finally, the portable sequencer 6 is used to generate genomic data in real time, and based on the rapid data analysis algorithm configured in the portable sequencer 6, functional gene annotation is realized to complete microbial gene detection.

[0053] In this solution, by integrating the shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 on the integrated device 1, the integration degree of the device is high, which is convenient for carrying out. Moreover, the integrated device 1 can supply power to each instrument through the power supply module 7, meeting the requirements of long-term field detection. Furthermore, based on the integration of each component on the integrated device 1, multiple steps in genomic and functional gene detection can be completed on-site without returning to the laboratory for analysis, avoiding sample degradation or contamination caused by transportation and storage, ensuring the authenticity of data and the accuracy of test results. At the same time, a large amount of time cost is also reduced. The device has good rapid detection ability, and the detection efficiency of this device is significantly improved compared with traditional detection technologies.

[0054] In a specific embodiment, please refer to Figures 1 to 3 , the integrated device 1 may include an instrument box 11 with an openable cover 12, and the power supply module 7 is arranged in the instrument box 11.

[0055] Specifically, a flexible member 8 is arranged in the instrument box 11, and a plurality of grooves 81 are formed on the flexible member 8. The shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 are respectively detachably arranged in their corresponding grooves 81.

[0056] It can be understood that the shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 can be isolated from each other through the flexible member 8, and can be limited and buffered by the flexible member 8. When the device needs to be used, the cover 12 is opened to expose components such as the shaker 2 and the vortex mixer 3. The tester can take out each component for operation or directly operate in place, and use each component for gene detection through the method described above. After use, the cover 12 can be closed to limit components such as the shaker 2 and the vortex mixer 3 in the grooves 81 of the flexible member 8.

[0057] It should be noted that the "installation space" mentioned above can be understood as the accommodation space formed by the grooves 81 of the flexible member 8.

[0058] Exemplarily, the cover 12 can be hinged to the instrument box 11 through a hinge, so as to be opened and closed in a flip-up manner. A buckle 18 can be arranged on the instrument box 11. When the cover 12 is closed, the cover 12 can be buckled and fixed to the instrument box 11 through the buckle 18. Part of the buckle 18 is located on the cover 12, and part is located on the instrument box 11.

[0059] Exemplarily, the power supply module 7 may include a battery 71, multiple cables, and multiple power supply interfaces (the cables and power supply interfaces are not shown in the figure). The battery 71 can be electrically connected to different power supply interfaces through different cables. Each of the multiple power supply interfaces corresponds to an instrument that needs to receive power. Each power supply interface can be disposed in the corresponding groove 81 of the corresponding instrument, so that when the instrument is placed in the groove 81, it can be connected to the power supply interface for charging. A charging interface (not shown in the figure) electrically connected to the battery 71 can be provided on the instrument box 11, so that the battery 71 can be charged by connecting to an external power supply through the charging interface.

[0060] Exemplarily, the material of the flexible member 8 includes but is not limited to sponge, silica gel, rubber, high-temperature resistant polymer foam, ceramic fiber, etc. The battery 71 can be a lithium battery.

[0061] In this embodiment, the shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 are all arranged in a detachable form, which is convenient for selecting and replacing components according to needs and has good flexibility.

[0062] In a specific embodiment, the flexible member 8 is detachably disposed in the instrument box 11, and at least one of the shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 is detachably disposed in the groove 81 in an interference fit manner with the flexible member 8.

[0063] Exemplarily, the size of the flexible member 8 can be exactly adapted to the internal size of the instrument box 11, so as to completely fill the internal space of the instrument box 11. The flexible member 8 can be stably disposed in the instrument box 11 based on the gravity of each instrument placed in its groove 81.

[0064] Preferably, the shaker 2, the vortex mixer 3, the DNA extraction component 4, the mass spectrometer 5, and the portable sequencer 6 are all detachably disposed in the groove 81 in an interference fit manner with the flexible member 8. The shape of the groove 81 is adapted to its corresponding component, but the size is slightly smaller than the corresponding component, so as to achieve an interference fit with the corresponding component. Exemplarily, please refer to Figure 4 the following table to introduce a specific setting situation of the size of the groove 81 of the flexible member 8.

[0065] Flexible Member Groove Size Table

[0066]

[0067]

[0068] It should be noted that the "serial number" in the table is the same as Figure 4The numbers in it correspond one by one. The "graphic" in the table represents the groove type of the groove 81. The "length" of the ellipse in the table can be understood as the major axis, the "width" of the ellipse can be understood as the minor axis, and the "height" can be understood as the depth of the groove 81.

[0069] In this embodiment, based on the detachable setting of the flexible member 8, the flexible member 8 with different grooves 81 can be flexibly replaced to adapt to various components of different models by changing the shape of the groove 81, having good flexibility and adaptability.

[0070] In a specific embodiment, in view of the inconvenient charging in the wild, the device has been adjusted accordingly. Please refer to Figure 1 , a solar panel 9 is provided outside the integrated device 1, and the solar panel 9 is electrically connected to the power supply module 7.

[0071] Exemplarily, in the embodiment where the integrated device 1 includes the instrument box 11 and the power supply module 7 includes the battery 71, the solar panel 9 can be arranged on the instrument box 11, and the solar panel 9 is electrically connected to the battery 71 of the power supply module 7, so that the device can convert light energy into electrical energy through the solar panel 9 and collect it through the battery 71. Thus, the portable microbial gene rapid detection device provided by this embodiment supports external solar panel power supply in addition to being powered by the built-in battery 71, so as to be able to adapt to long-term field applications.

[0072] Optionally, the number of the solar panels 9 can be one or more.

[0073] In a specific embodiment, in view of the application in a low-temperature environment, the device has been adjusted accordingly. Please refer to Figure 3 , a temperature sensor 10 is arranged in the instrument box 11, and an electric heater 13 is arranged in the wall of at least one groove 81. The temperature sensor 10 and the electric heater 13 are both electrically connected to the power supply module 7, and the electric heater 13 is communicatively connected to the temperature sensor 10 for turning on heating when the internal temperature of the instrument box 11 is lower than the preset temperature.

[0074] Exemplarily, in the embodiment where the power supply module 7 includes the battery 71, the temperature sensor 10 and the electric heater 13 are both electrically connected to the battery 71 to work normally under the power supply of the battery 71. The electric heater 13 includes but is not limited to an electric heating wire or an electric heating sheet, etc. The electric heater 13 can be arranged in the wall of the groove 81 corresponding to the mass spectrometer 5 and the portable sequencer 6, so as to ensure that the mass spectrometer 5 and the portable sequencer 6 can be directly heated by the electric heater 13.

[0075] Optionally, the preset temperature can be determined according to the optimal operating temperature of instruments such as the mass spectrometer 5 and the portable sequencer 6. Different preset temperatures can be set for different instrument models, and this embodiment does not make specific limitations in this regard. For example, the preset temperature can be set to 15°C. When the temperature sensor 10 detects that the temperature inside the instrument box 11 is lower than 15°C, the electric heater 13 is turned on to heat the instrument and increase the temperature inside the instrument box 11. In practical applications, the temperature can be judged by the temperature sensor 10 and the electric heater 13 can be used for heating to keep the temperature inside the instrument box 11 between 18°C and 30°C, which is beneficial to ensuring the normal operation of the mass spectrometer 5 and the portable sequencer 6, and further improving the accuracy of the detection results. Moreover, the instrument is not easily affected by external low temperatures and can perform gene detection work at any time, which greatly improves the convenience of equipment use and further reduces the time cost of detection.

[0076] In this embodiment, the flexible member 8 is preferably made of heat-resistant materials such as silica gel, high-temperature resistant polymer foam or ceramic fiber.

[0077] In a specific embodiment, for the case of being applied to a low-pressure environment, the device has been correspondingly adjusted. Please refer to Figure 3 again. A pressure relief valve 14 for adjusting the internal pressure of the instrument box 11 can also be provided on the instrument box 11. In the low-temperature and low-pressure environment of high-altitude areas, it is necessary to ensure that the instrument box 11 has good sealing performance. Therefore, the instrument box 11 may expand due to the internal air pressure being greater than the external air pressure. Through the setting of the pressure relief valve 14, the air pressure inside and outside the instrument box 11 can be balanced, thus solving the problem of the instrument box 11 expanding in a low-pressure environment.

[0078] In a specific embodiment, the length range of the instrument box 11 can be between 45 cm and 55 cm, the width range of the instrument box 11 can be between 25 cm and 35 cm, and the height range of the instrument box 11 can be between 15 cm and 25 cm.

[0079] Exemplarily, the instrument box 11 can be in the shape of a cuboid, and the length of the instrument box 11 is set to 50 cm, the width is set to 30 cm, and the height is set to 20 cm. Such a size setting can well adapt to the sizes of various components, is convenient to carry, and such a design makes the weight of the device within 10 kg, which is relatively light and provides a good user experience.

[0080] In a specific embodiment, at least one of the cover 12 and the instrument box 11 is provided with a sealing member, which is used to achieve a sealed connection between the cover 12 and the instrument box 11 when the cover 12 is covered on the instrument box 11.

[0081] Preferably, sealing members are provided on both the cover body 12 and the instrument box 11. Exemplarily, the sealing member can be a sealing ring 15. The edges of the cover body 12 and the instrument box 11 are both wound with sealing rings 15, and the two sealing rings 15 correspond to each other. When the cover body 12 is covered on the instrument box 11, the two sealing rings 15 are pressed against each other, thereby realizing the sealed connection between the cover body 12 and the instrument box 11.

[0082] In this embodiment, the sealed connection between the cover body 12 and the instrument box 11 is realized by setting the sealing member, which can effectively prevent cold air, dust and water vapor from entering the instrument box 11, improve the heat preservation effect in the instrument box 11, and avoid the influence of the complex external environment on the inside of the instrument box 11, and the reliability of the equipment is better.

[0083] Furthermore, a heat preservation and buffer member 16 can be provided on the side of the cover body 12 close to the instrument box 11. When the cover body 12 is covered on the instrument box 11, the heat preservation and buffer member 16 is located inside the instrument box 11, and each component in the instrument box 11 is located between the flexible member 8 and the heat preservation and buffer member 16.

[0084] Exemplarily, the heat preservation and buffer member 16 includes but is not limited to a sponge layer, an aerogel heat preservation material layer, etc.

[0085] In a specific embodiment, the instrument box 11 is made of any one or a combination of carbon fiber composite materials, aluminum alloy materials, and titanium alloy materials, so that while the instrument box 11 is light and easy to carry, it also takes into account strength and is very suitable for use in the wild at high altitudes.

[0086] In a specific embodiment, a handle 17 for human hands to hold is provided on the integrated device 1, thereby further improving the convenience of carrying. In the embodiment where the integrated device 1 includes the instrument box 11, the handle 17 is provided on the instrument box 11.

[0087] The actual application results of this equipment are shown exemplarily below. Please refer to Figure 5, are sampling points and metagenomic annotations. This study was conducted using the portable microbial gene rapid detection device provided by the present invention at Qilian Mountain A (38°13′N–38°16′N to 99°50′E–99°53′E) above 3000 meters altitude. Three locations were circled in A, and ecological regions were represented by different symbols, where HA represents high-altitude regions, MA represents mid-altitude regions, and LA represents low-altitude regions. B is a wind rose diagram (stacked percentage) of gene functions identified by the eggNOG mapper. The range of the arranged e-core is from 2.2E-57 to 5.3E-7, with a score of 113 ± 48 (mean ± sd), and the wind rose grade is 5% (per circle). C shows the Kaiju classification and gene function results. The gray circles represent the proportion of microbial classification (at the class level), and the corresponding gene functions are shown in the stacked bar chart (in units of 100%).

[0088] As Figure 5 shown, by applying the portable microbial gene rapid detection device provided by the present invention, Legionella pneumophila subsp (Legionella pneumophila subspecies), a typical human pathogenic bacterium that can persist in the aquatic environment, was found in the meltwater sample. For eukaryotic ribosomal RNA detection, this study found Blastomyces dermatitidis, a fungus that lives in moist soil, usually near rivers and lakes, and causes severe blastomycosis. Hydatigera kamiyai (Taenia kamiyai) was also found in the sample. This device can provide comprehensive genomic information or functional gene annotation results of the microbial community. The device is applicable to various environmental samples, such as glacier meltwater, soil, and wastewater, and can provide real-time microbial classification and functional gene annotation results.

[0089] The results of this task indicate that nanopore metagenomic sequencing can provide reliable prokaryotic classification in or between in-situ sequencing studies. Therefore, more applications in this field can be encouraged within a faster turnaround time. However, we recommend accumulating at least 400 ng of nucleic acid (after extraction) before on-site sequencing and maximizing the preparation efficiency of the nanopore library to obtain better resolution.

[0090] In summary, a portable rapid microbial gene detection device provided by the present invention integrates a shaker, a vortex mixer, a DNA extraction component, a quality detector, and a portable sequencer on an integrated device, resulting in a high degree of integration of the device, making it convenient to carry outdoors. Moreover, the integrated device can supply power to each instrument through a power supply module, meeting the needs of long-term field detection. Additionally, based on the integration of various components on the integrated device, this device can complete multiple steps in genomic and functional gene detection on-site without the need to return to the laboratory for analysis, avoiding sample degradation or contamination caused by transportation and storage, ensuring the authenticity of data and the accuracy of detection results. At the same time, it also reduces a large amount of time costs. The device has good rapid detection capabilities, and compared with traditional detection technologies, the detection efficiency of this device has been significantly improved.

[0091] Furthermore, the shaker, the vortex mixer, the DNA extraction component, the quality detector, and the portable sequencer are all arranged in a detachable form, which facilitates the selection and replacement of components according to needs and has good flexibility. The flexible component can also be arranged in a detachable form, and the flexible component with different grooves can be flexibly replaced to adapt to various components of different models by changing the shape of the grooves.

[0092] Furthermore, in view of the inconvenient charging in the wild, this device can convert light energy into electrical energy through a solar panel and collect it through a battery. Thus, in addition to being powered by the built-in battery, this device also supports external solar panel power supply, enabling it to adapt to long-term field applications.

[0093] Furthermore, in the case of being applied to a low-temperature environment, this device can judge the temperature through a temperature sensor and heat through an electric heater to keep the temperature inside the instrument box within a certain range, which is beneficial to ensuring the normal operation of the quality detector and the portable sequencer, and further improving the accuracy of detection results. Moreover, the instrument is not easily affected by the external low temperature and can perform gene detection work at any time, greatly improving the convenience of device use and further reducing the time cost of detection.

[0094] Furthermore, in the case of being applied to a low-pressure environment, through the setting of a pressure relief valve, this device can balance the air pressure inside and outside the instrument box, thus solving the problem of the instrument box expanding in a low-pressure environment.

[0095] Furthermore, the length range of the instrument box can be between 45 cm and 55 cm, the width range can be between 25 cm and 35 cm, the height range can be between 15 cm and 25 cm, and the weight of the instrument box is within 10 kg, being relatively light and providing a good use experience.

[0096] Note that the above is only a preferred embodiment of the present invention and the technical principles applied. Those skilled in the art will understand that the present invention is not limited to the specific embodiments here, and various obvious changes, re-adjustments, and substitutions can be made by those skilled in the art without departing from the protection scope of the present invention. Therefore, although the present invention has been described in more detail through the above embodiments, the present invention is not limited to the above embodiments only. Without departing from the concept of the present invention, more other equivalent embodiments can be included, and the scope of the present invention is determined by the scope of the appended claims.

[0097] The above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A portable rapid microbial gene detection device, characterized in that: include: Integrated devices, oscillators, mediators, DNA extractors, mass analyzers and portable sequencers; The integrated device has a plurality of installation spaces inside, and the oscillator, the mediator, the DNA extractor, the mass measuring instrument and the portable sequencer are respectively arranged in the corresponding installation spaces; The integrated device has a power supply module, which is electrically connected to the oscillator, the mediator, the mass analyzer and the portable sequencer to supply power to the instruments electrically connected thereto.

2. A portable microbial gene rapid detection device according to claim 1, characterized in that: A solar panel is arranged outside the integrated device, and the solar panel is electrically connected to the power supply module.

3. A portable microbial gene rapid detection device according to claim 1, characterized in that: The integrated device comprises an instrument box having an openable cover, and the power supply module is arranged in the instrument box; A flexible part is arranged in the instrument box, and a plurality of grooves are opened on the flexible part. The oscillator, the mediator, the DNA extractor, the mass measuring instrument and the portable sequencer are respectively and detachably arranged in the corresponding grooves.

4. A portable microbial gene rapid detection device according to claim 3, characterized in that: A temperature sensor is provided in the instrument box, and an electric heater is provided in the groove wall of at least one of the grooves. The temperature sensor and the electric heater are electrically connected to the power supply module. The electric heater is communicatively connected to the temperature sensor and is used to start heating when the internal temperature of the instrument box is lower than a preset temperature.

5. A portable microbial gene rapid detection device according to claim 3, characterized in that: The instrument box is provided with a pressure relief valve for adjusting the internal pressure of the instrument box.

6. A portable microbial gene rapid detection device according to claim 3, characterized in that: The flexible member is detachably arranged in the instrument box; And / or, at least one of the oscillator, the mediator, the DNA extractor, the mass measuring instrument and the portable sequencer is detachably arranged in the groove by means of interference fit with the flexible part.

7. A portable microbial gene rapid detection device according to claim 3, characterized in that: The length of the instrument box ranges from 45 cm to 55 cm; And / or, the width of the instrument box ranges from 25 cm to 35 cm; And / or, the height of the instrument box ranges from 15 cm to 25 cm.

8. A portable microbial gene rapid detection device according to claim 3, characterized in that: At least one of the cover body and the instrument box is provided with a sealing member, and the sealing member is used to achieve a sealed connection between the cover body and the instrument box when the cover body is covered on the instrument box; And / or, a heat-insulating buffer is provided on one side of the cover body close to the instrument box.

9. A portable microbial gene rapid detection device according to claim 3, characterized in that: The instrument box is made of any one or a combination of carbon fiber composite materials, aluminum alloy materials, and titanium alloy materials.

10. A portable microbial gene rapid detection device according to claim 1, characterized in that: The integrated device is provided with a handle for being held by a human hand.

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